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Nanoparticle delivery of innate immune agonists combined with senescence-inducing agents promotes T cell control of pancreatic cancer 纳米颗粒递送先天性免疫激动剂与衰老诱导剂相结合,可促进 T 细胞对胰腺癌的控制。
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-08-28 DOI: 10.1126/scitranslmed.adj9366
Loretah Chibaya, Kelly D. DeMarco, Christina F. Lusi, Griffin I. Kane, Meghan L. Brassil, Chaitanya N. Parikh, Katherine C. Murphy, Shreya R. Chowdhury, Junhui Li, Boyang Ma, Tiana E. Naylor, Julia Cerrutti, Haruka Mori, Miranda Diaz-Infante, Jessica Peura, Jason R. Pitarresi, Lihua Julie Zhu, Katherine A. Fitzgerald, Prabhani U. Atukorale, Marcus Ruscetti
Pancreatic ductal adenocarcinoma (PDAC) has quickly risen to become the third leading cause of cancer-related death in the United States. This is in part because of its fibrotic tumor microenvironment (TME) that contributes to poor vascularization and immune infiltration and subsequent chemo- and immunotherapy failure. Here, we investigated an immunotherapy approach combining delivery of stimulator of interferon genes (STING) and Toll-like receptor 4 (TLR4) innate immune agonists by lipid-based nanoparticle (NP) coencapsulation with senescence-inducing RAS-targeted therapies, which can remodel the immune suppressive PDAC TME through the senescence-associated secretory phenotype. Treatment of transplanted and autochthonous PDAC mouse models with these regimens led to enhanced uptake of NPs by multiple cell types in the PDAC TME, induction of type I interferon and other proinflammatory signaling pathways, increased antigen presentation by tumor cells and antigen-presenting cells, and subsequent activation of both innate and adaptive immune responses. This two-pronged approach produced potent T cell–driven and type I interferon–mediated tumor regression and long-term survival in preclinical PDAC models dependent on both tumor and host STING activation. STING and TLR4-mediated type I interferon signaling was also associated with enhanced natural killer and CD8+ T cell immunity in human PDAC samples. Thus, combining localized immune agonist delivery with systemic tumor-targeted therapy can orchestrate a coordinated type I interferon–driven innate and adaptive immune response with durable antitumor efficacy against PDAC.
胰腺导管腺癌(PDAC)已迅速跃升为美国癌症相关死亡的第三大原因。这部分是由于其纤维化的肿瘤微环境(TME)导致血管化和免疫浸润不良,进而导致化疗和免疫治疗失败。在这里,我们研究了一种免疫疗法,它通过脂基纳米粒子(NP)包被将干扰素基因刺激因子(STING)和Toll样受体4(TLR4)先天性免疫激动剂与衰老诱导RAS靶向疗法相结合,从而通过衰老相关分泌表型重塑免疫抑制性PDAC TME。用这些疗法治疗移植和自体PDAC小鼠模型会增强PDAC TME中多种类型细胞对NPs的吸收,诱导I型干扰素和其他促炎症信号通路,增加肿瘤细胞和抗原递呈细胞的抗原递呈,继而激活先天性和适应性免疫反应。这种双管齐下的方法在临床前 PDAC 模型中产生了强效的 T 细胞驱动和 I 型干扰素介导的肿瘤消退和长期存活,这取决于肿瘤和宿主 STING 的激活。STING 和 TLR4 介导的 I 型干扰素信号也与人类 PDAC 样本中增强的自然杀伤细胞和 CD8+ T 细胞免疫有关。因此,将局部免疫激动剂给药与全身性肿瘤靶向治疗相结合,可以协调I型干扰素驱动的先天性和适应性免疫反应,对PDAC具有持久的抗肿瘤疗效。
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引用次数: 0
Next-generation CAR T cell therapies for glioblastoma 治疗胶质母细胞瘤的新一代 CAR T 细胞疗法。
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-08-28 DOI: 10.1126/scitranslmed.adp2660
Joshua D. Bernstock, Jakob V. E. Gerstl, Pablo A. Valdés, Gregory K. Friedman, E. Antonio Chiocca
Interim results from two phase 1 trials demonstrate progress in the use of chimeric antigen receptor (CAR) T cell therapy for recurrent glioblastoma (GBM).
两项一期试验的中期结果表明,嵌合抗原受体(CAR)T细胞疗法在治疗复发性胶质母细胞瘤(GBM)方面取得了进展。
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引用次数: 0
Microglia-specific IL-10 gene delivery inhibits neuroinflammation and neurodegeneration in a mouse model of Parkinson’s disease 小胶质细胞特异性 IL-10 基因递送可抑制帕金森病小鼠模型中的神经炎症和神经退行性变。
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-08-21 DOI: 10.1126/scitranslmed.adm8563
Simone Bido, Melania Nannoni, Sharon Muggeo, Diana Gambarè, Giorgia Ruffini, Edoardo Bellini, Laura Passeri, Silvia Iaia, Mirko Luoni, Martino Provinciali, Serena Gea Giannelli, Francesca Giannese, Dejan Lazarevic, Silvia Gregori, Vania Broccoli
Neuroinflammation plays a key role in exacerbating dopaminergic neuron (DAN) loss in Parkinson’s disease (PD). However, it remains unresolved how to effectively normalize this immune response given the complex interplay between the innate and adaptive immune responses occurring within a scarcely accessible organ like the brain. In this study, we uncovered a consistent correlation between neuroinflammation, brain parenchymal lymphocytes, and DAN loss among several commonly used mouse models of PD generated by a variety of pathological triggers. We validated a viral therapeutic approach for the microglia-specific expression of interleukin 10 (IL-10) to selectively mitigate the excessive inflammatory response. We found that this approach induced a local nigral IL-10 release that alleviated DAN loss in mice overexpressing the human SNCA gene in the substantia nigra. Single-cell transcriptomics revealed that IL-10 induced the emergence of a molecularly distinct microglial cell state, enriched in markers of cell activation with enhanced expression of prophagocytic pathways. IL-10 promoted microglial phagocytotic and clearance activities in vitro and reduced αSYN aggregate burden in the nigral area in mice overexpressing SNCA. Furthermore, IL-10 stimulated the differentiation of CD4+ T lymphocytes into active T regulatory cells and promoted inhibitory characteristics in CD8+ T cells. In summary, our results show that local and microglia-specific IL-10 transduction elicited strong immunomodulation in the nigral tissue with enhanced suppression of lymphocyte toxicity that was associated with DAN survival. These results offer insights into the therapeutic benefits of IL-10 and showcase a promising gene delivery approach that could minimize undesired side effects.
神经炎症在加剧帕金森病(PD)多巴胺能神经元(DAN)丢失方面起着关键作用。然而,鉴于先天性免疫反应和适应性免疫反应之间复杂的相互作用发生在大脑这样一个几乎无法触及的器官中,如何有效地使这种免疫反应正常化仍是一个悬而未决的问题。在这项研究中,我们发现了神经炎症、脑实质淋巴细胞和 DAN 丢失之间的一致相关性,这种相关性存在于几种常用的由各种病理诱因产生的帕金森病小鼠模型中。我们验证了一种病毒治疗方法,即小胶质细胞特异性表达白细胞介素 10(IL-10),以选择性地减轻过度炎症反应。我们发现,这种方法能诱导局部黑质 IL-10 的释放,从而减轻黑质中过表达人类 SNCA 基因的小鼠的 DAN 缺失。单细胞转录组学显示,IL-10诱导了一种分子上截然不同的小胶质细胞状态的出现,这种状态富含细胞活化的标记物,并增强了亲吞噬途径的表达。IL-10在体外促进了小胶质细胞的吞噬和清除活动,并减少了过表达SNCA的小鼠黑质区的αSYN聚集负荷。此外,IL-10 还能刺激 CD4+ T 淋巴细胞分化为活跃的 T 调节细胞,并促进 CD8+ T 细胞的抑制特性。总之,我们的研究结果表明,局部和小胶质细胞特异性 IL-10 转导在黑质组织中引起了强烈的免疫调节,增强了对淋巴细胞毒性的抑制,这与 DAN 的存活有关。这些结果让我们深入了解了IL-10的治疗功效,并展示了一种可将不良副作用降至最低的前景广阔的基因递送方法。
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引用次数: 0
Intestinal helminth infection impairs vaccine-induced T cell responses and protection against SARS-CoV-2 in mice 肠道蠕虫感染会损害疫苗诱导的 T 细胞反应和小鼠对 SARS-CoV-2 的保护。
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-08-21 DOI: 10.1126/scitranslmed.ado1941
Pritesh Desai, Courtney E. Karl, Baoling Ying, Chieh-Yu Liang, Tamara Garcia-Salum, Ana Carolina Santana, Felipe ten-Caten, Joseph F. Urban Jr., Sayda M. Elbashir, Darin K. Edwards, Susan P. Ribeiro, Larissa B. Thackray, Rafick P. Sekaly, Michael S. Diamond
Although vaccines have reduced the burden of COVID-19, their efficacy in helminth infection–endemic areas is not well characterized. We evaluated the impact of infection by Heligmosomoides polygyrus bakeri (Hpb), a murine intestinal roundworm, on the efficacy of an mRNA vaccine targeting the Wuhan-1 spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in mice. Although immunization generated similar B cell responses in Hpb-infected and uninfected mice, polyfunctional CD4+ and CD8+ T cell responses were markedly reduced in Hpb-infected mice. Hpb-infected and mRNA-vaccinated mice were protected against the ancestral SARS-CoV-2 strain WA1/2020, but control of lung infection was diminished against an Omicron variant compared with animals immunized without Hpb infection. Helminth-mediated suppression of spike protein–specific CD8+ T cell responses occurred independently of signal transducer and activator of transcription 6 (STAT6) signaling, whereas blockade of interleukin-10 (IL-10) rescued vaccine-induced CD8+ T cell responses. Together, these data show that, in mice, intestinal helminth infection impaired vaccine-induced T cell responses through an IL-10 pathway, which compromised protection against antigenically drifted SARS-CoV-2 variants.
尽管疫苗已经减轻了 COVID-19 的负担,但其在蠕虫感染流行地区的疗效还没有得到很好的描述。我们评估了小鼠肠道蛔虫 Heligmosomoides polygyrus bakeri(Hpb)感染对针对严重急性呼吸系统综合征冠状病毒 2(SARS-CoV-2)武汉-1 尖峰蛋白的 mRNA 疫苗在小鼠中的疗效的影响。虽然免疫接种在感染 Hpb 和未感染 Hpb 的小鼠中产生了相似的 B 细胞反应,但感染 Hpb 的小鼠的多功能 CD4+ 和 CD8+ T 细胞反应明显降低。与未感染 Hpb 的免疫小鼠相比,感染 Hpb 和接种 mRNA 疫苗的小鼠对 SARS-CoV-2 祖毒株 WA1/2020 有保护作用,但对 Omicron 变异株的肺部感染控制能力较弱。螺旋体介导的尖峰蛋白特异性 CD8+ T 细胞应答抑制与信号转导和转录激活因子 6(STAT6)信号转导无关,而阻断白细胞介素-10(IL-10)可挽救疫苗诱导的 CD8+ T 细胞应答。这些数据共同表明,在小鼠体内,肠道蠕虫感染通过IL-10途径损害了疫苗诱导的T细胞反应,从而削弱了对抗原漂移的SARS-CoV-2变体的保护作用。
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引用次数: 0
Repeated Omicron exposures redirect SARS-CoV-2–specific memory B cell evolution toward the latest variants 反复暴露于 Omicron 病毒会使 SARS-CoV-2 特异性记忆 B 细胞向最新变种进化。
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-08-21 DOI: 10.1126/scitranslmed.adp9927
Ryutaro Kotaki, Saya Moriyama, Shintaro Oishi, Taishi Onodera, Yu Adachi, Eita Sasaki, Kota Ishino, Miwa Morikawa, Hiroaki Takei, Hidenori Takahashi, Tomohiro Takano, Ayae Nishiyama, Kohei Yumoto, Kazutaka Terahara, Masanori Isogawa, Takayuki Matsumura, Masaharu Shinkai, Yoshimasa Takahashi
Immunological imprinting by ancestral SARS-CoV-2 strains is thought to impede the robust induction of Omicron-specific humoral responses by Omicron-based booster vaccines. Here, we analyzed the specificity and neutralization activity of memory B (Bmem) cells after repeated BA.5 exposure in individuals previously imprinted by ancestral strain–based mRNA vaccines. After a second BA.5 exposure, Bmem cells with BA.5 spike protein–skewed reactivity were promptly elicited, correlating with preexisting antibody titers. Clonal lineage analysis identified BA.5-skewed Bmem cells that had redirected their specificity from the ancestral strain to BA.5 through somatic hypermutations. Moreover, Bmem cells with redirected BA.5 specificity exhibited accelerated development compared with de novo Bmem cells derived from naïve repertoires. This redirected BA.5 specificity demonstrated greater resilience to viral point mutation and adaptation to recent Omicron variants HK.3 and JN.1, months after the second BA.5 exposure, suggesting that existing Bmem cells elicited by older vaccines can redirect their specificity toward newly evolving variants.
人们认为,SARS-CoV-2祖先毒株的免疫印记阻碍了以Omicron为基础的加强疫苗诱导Omicron特异性体液反应的能力。在这里,我们分析了先前被基于祖先毒株的 mRNA 疫苗印记的个体在重复接种 BA.5 后记忆 B(Bmem)细胞的特异性和中和活性。在第二次接触 BA.5 后,具有 BA.5 尖峰蛋白偏斜反应性的记忆 B 细胞被迅速激发,这与之前存在的抗体滴度相关。克隆谱系分析发现,BA.5偏向的Bmem细胞通过体细胞高突变将其特异性从祖先菌株重定向到了BA.5。此外,与来源于原始基因库的新生 Bmem 细胞相比,具有 BA.5 重定向特异性的 Bmem 细胞表现出更快的发育速度。这种重新定向的 BA.5 特异性在第二次接触 BA.5 几个月后表现出更强的抗病毒点突变能力,并能适应最近的 Omicron 变种 HK.3 和 JN.1,这表明旧疫苗激发的现有 Bmem 细胞能将其特异性重新定向到新进化的变体上。
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引用次数: 0
Stress-induced eosinophil activation contributes to postoperative morbidity and mortality after lung resection 压力导致的嗜酸性粒细胞活化是肺切除术后发病率和死亡率的原因之一。
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-08-21 DOI: 10.1126/scitranslmed.adl4222
Zhongcheng Mei, May A. Khalil, Yizhan Guo, Dongge Li, Anirban Banerjee, Mojtaba Taheri, Christina M. Kratzmeier, Kelly Chen, Christine L. Lau, Irina G. Luzina, Sergei P. Atamas, Sivaveera Kandasamy, Daniel Kreisel, Andrew E. Gelman, Elizabeth A. Jacobsen, Alexander Sasha Krupnick
Respiratory failure occurs more frequently after thoracic surgery than abdominal surgery. Although the etiology for this complication is frequently attributed to underlying lung disease present in patients undergoing thoracic surgery, this notion is often unfounded because many patients with normal preoperative pulmonary function often require prolonged oxygen supplementation even after minimal resection of lung tissue. Using a murine model of pulmonary resection and peripheral blood samples from patients undergoing resection of the lung or abdominal organs, we demonstrated that lung surgery initiates a proinflammatory loop that results in damage to the remaining lung tissue, noncardiogenic pulmonary edema, hypoxia, and even death. Specifically, we demonstrated that resection of murine lung tissue increased concentrations of the homeostatic cytokine interleukin-7, which led to local and systemic activation of type 2 innate lymphoid cells. This process activated lung-resident eosinophils and facilitated stress-induced eosinophil maturation in the bone marrow in a granulocyte-macrophage colony-stimulating factor–dependent manner, resulting in systemic eosinophilia in both mice and humans. Up-regulation of inducible nitric oxide synthase in lung-resident eosinophils led to tissue nitrosylation, pulmonary edema, hypoxia, and, at times, death. Disrupting this activation cascade at any stage ameliorated deleterious outcomes and improved survival after lung resection in the mouse model. Our data suggest that repurposing US Food and Drug Administration–approved eosinophil-targeting strategies may potentially offer a therapeutic intervention to improve outcomes for patients who require lung resection for benign or malignant etiology.
与腹部手术相比,胸部手术后呼吸衰竭的发生率更高。虽然这种并发症的病因经常被归结为接受胸部手术的患者存在潜在的肺部疾病,但这一观点往往是没有根据的,因为许多术前肺功能正常的患者即使在肺组织被微小切除后也经常需要长时间的氧气补充。我们利用肺切除小鼠模型和肺部或腹部器官切除患者的外周血样本证明,肺部手术会引发促炎循环,导致剩余肺组织受损、非心源性肺水肿、缺氧甚至死亡。具体来说,我们证明切除小鼠肺组织会增加同源细胞因子白细胞介素-7的浓度,从而导致局部和全身2型先天性淋巴细胞的活化。这一过程激活了肺驻留的嗜酸性粒细胞,并以粒细胞-巨噬细胞集落刺激因子依赖的方式促进了压力诱导的骨髓中嗜酸性粒细胞的成熟,从而导致小鼠和人类全身性嗜酸性粒细胞增多。肺驻留嗜酸性粒细胞中诱导型一氧化氮合酶的上调导致组织亚硝基化、肺水肿、缺氧,有时甚至导致死亡。在小鼠模型中,在任何阶段破坏这一活化级联都能改善有害结果,提高肺切除后的存活率。我们的数据表明,重新利用美国食品药品管理局批准的嗜酸性粒细胞靶向策略有可能提供一种治疗干预措施,改善因良性或恶性病因需要进行肺切除术的患者的预后。
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引用次数: 0
Modified C-type natriuretic peptide normalizes tumor vasculature, reinvigorates antitumor immunity, and improves solid tumor therapies 改良 C 型钠尿肽可使肿瘤血管正常化,重振抗肿瘤免疫力,并改善实体瘤疗法。
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-08-21 DOI: 10.1126/scitranslmed.adn0904
Zhen Lu, Ioannis Verginadis, Motofumi Kumazoe, Gerardo M. Castillo, Yao Yao, Rebecca E. Guerra, Sandra Bicher, Menghao You, George McClung, Rong Qiu, Zebin Xiao, Zhen Miao, Subin S. George, Daniel P. Beiting, Takashi Nojiri, Yasutake Tanaka, Yoshinori Fujimura, Hiroaki Onda, Yui Hatakeyama, Akiko Nishimoto-Ashfield, Katrina Bykova, Wei Guo, Yi Fan, Nikolay M. Buynov, J. Alan Diehl, Ben Z. Stanger, Hirofumi Tachibana, Terence P. Gade, Ellen Puré, Constantinos Koumenis, Elijah M. Bolotin, Serge Y. Fuchs
Deficit of oxygen and nutrients in the tumor microenvironment (TME) triggers abnormal angiogenesis that produces dysfunctional and leaky blood vessels, which fail to adequately perfuse tumor tissues. Resulting hypoxia, exacerbation of metabolic disturbances, and generation of an immunosuppressive TME undermine the efficacy of anticancer therapies. Use of carefully scheduled angiogenesis inhibitors has been suggested to overcome these problems and normalize the TME. Here, we propose an alternative agonist-based normalization approach using a derivative of the C-type natriuretic peptide (dCNP). Multiple gene expression signatures in tumor tissues were affected in mice treated with dCNP. In several mouse orthotopic and subcutaneous solid tumor models including colon and pancreatic adenocarcinomas, this well-tolerated agent stimulated formation of highly functional tumor blood vessels to reduce hypoxia. Administration of dCNP also inhibited stromagenesis and remodeling of the extracellular matrix and decreased tumor interstitial fluid pressure. In addition, treatment with dCNP reinvigorated the antitumor immune responses. Administration of dCNP decelerated growth of primary mouse tumors and suppressed their metastases. Moreover, inclusion of dCNP into the chemo-, radio-, or immune-therapeutic regimens increased their efficacy against solid tumors in immunocompetent mice. These results demonstrate the proof of principle for using vasculature normalizing agonists to improve therapies against solid tumors and characterize dCNP as the first in class among such agents.
肿瘤微环境(TME)中氧气和营养物质的缺乏会引发异常血管生成,从而产生功能障碍和渗漏血管,无法充分灌注肿瘤组织。由此导致的缺氧、代谢紊乱的加剧以及免疫抑制性肿瘤微环境的产生,都会削弱抗癌疗法的疗效。有人建议使用精心安排的血管生成抑制剂来克服这些问题,使 TME 恢复正常。在这里,我们提出了另一种基于激动剂的正常化方法,即使用 C 型钠尿肽(dCNP)的衍生物。使用 dCNP 治疗的小鼠肿瘤组织中的多个基因表达特征受到了影响。在包括结肠癌和胰腺癌在内的几种小鼠正位和皮下实体瘤模型中,这种耐受性良好的药物可刺激高功能肿瘤血管的形成,从而减少缺氧。服用 dCNP 还能抑制细胞外基质的形成和重塑,降低肿瘤间质压力。此外,使用 dCNP 治疗还能重振抗肿瘤免疫反应。服用 dCNP 可减缓小鼠原发性肿瘤的生长并抑制其转移。此外,在化疗、放射治疗或免疫治疗方案中加入 dCNP 还能提高免疫功能健全的小鼠对实体瘤的疗效。这些结果证明了使用血管正常化激动剂改善实体瘤疗法的原理,并使 dCNP 成为此类药物中的首例。
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引用次数: 0
An identity crisis for lung cancer cells 肺癌细胞的身份危机
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-08-14 DOI: 10.1126/scitranslmed.adp9616
Álvaro Quintanal-Villalonga
Omic analysis of clinical specimens undergoing histological transformation defines targetable drivers to prevent plasticity and treatment resistance.
对发生组织学转化的临床标本进行 Omic 分析,确定了防止可塑性和耐药性的靶向驱动因素。
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引用次数: 0
Natural killer cells promote neutrophil extracellular traps and restrain macular degeneration in mice 自然杀伤细胞促进中性粒细胞胞外捕获,抑制小鼠黄斑变性。
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-08-14 DOI: 10.1126/scitranslmed.adi6626
Xue Dong, Yinting Song, Yuming Liu, Xuejing Kou, Tianjing Yang, Samuel X. Shi, Kai He, Yiming Li, Ziqi Li, Xueming Yao, Ju Guo, Bohao Cui, Ziru Wu, Yi Lei, Mei Du, Mei Chen, Heping Xu, Qiang Liu, Fu-Dong Shi, Xiaohong Wang, Hua Yan
Neovascular age-related macular degeneration (nvAMD) is the leading cause of blindness in the elderly population. Although it is known that nvAMD is associated with focal inflammation, understanding of the precise immune components governing this process remains limited. Here, we identified natural killer (NK) cells as a prominent lymphocyte population infiltrating the perivascular space of choroidal neovascularization (CNV) lesions in patients with nvAMD and in mouse models. Olink proteomic analysis and single-cell RNA sequencing combined with knockout studies demonstrated the involvement of C-C chemokine receptor 5 (CCR5) in NK cell recruitment and extravasation at the CNV sites of mice. Depletion of NK cells or inhibition of activating receptor NK group 2, member D (NKG2D) inhibited the formation of neutrophil extracellular traps, increased vascular leakage, and exacerbated pathological angiogenesis, indicating that NK cells restrain pathogenesis in this mouse model. Age is the strongest risk factor for AMD, and we show that NK cells from aged human donors exhibited a less cytotoxic phenotype. NK cells from old mice exhibited compromised protective effects in the CNV mouse model. In addition, interleukin-2 complex–mediated expansion of NK cells improved CNV formation in mice. Collectively, our study highlights NK cells as a potential therapeutic target for patients with nvAMD.
新生血管性老年黄斑变性(nvAMD)是老年人失明的主要原因。尽管人们知道新生血管性老年黄斑变性与局灶性炎症有关,但对支配这一过程的精确免疫成分的了解仍然有限。在这里,我们发现自然杀伤(NK)细胞是渗入nvAMD患者和小鼠模型脉络膜新生血管(CNV)病变血管周围空间的主要淋巴细胞群。Olink蛋白组分析和单细胞RNA测序结合基因敲除研究证明,C-C趋化因子受体5(CCR5)参与了小鼠CNV部位的NK细胞招募和外渗。消耗NK细胞或抑制活化受体NK 2组D成员(NKG2D)可抑制中性粒细胞胞外捕获物的形成、增加血管渗漏并加剧病理性血管生成,这表明NK细胞抑制了该小鼠模型的发病机制。年龄是导致老年性视网膜病变的最大风险因素,而我们的研究表明,来自高龄人类供体的 NK 细胞表现出较低的细胞毒性表型。在 CNV 小鼠模型中,来自高龄小鼠的 NK 细胞表现出的保护作用也大打折扣。此外,白介素-2 复合物介导的 NK 细胞扩增改善了小鼠 CNV 的形成。总之,我们的研究强调了 NK 细胞是 nvAMD 患者的潜在治疗靶点。
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引用次数: 0
Acyl-CoA binding protein for the experimental treatment of anorexia 用于实验性治疗厌食症的 Acyl-CoA 结合蛋白。
IF 15.8 1区 医学 Q1 CELL BIOLOGY Pub Date : 2024-08-14 DOI: 10.1126/scitranslmed.adl0715
Hui Chen, Stéphanie Moriceau, Adrien Joseph, Francois Mailliet, Sijing Li, Virginie Tolle, Philibert Duriez, Roland Dardennes, Sylvère Durand, Vincent Carbonnier, Gautier Stoll, Allan Sauvat, Sylvie Lachkar, Fanny Aprahamian, Carolina Alves Costa Silva, Hui Pan, Léa Montégut, Gerasimos Anagnostopoulos, Flavia Lambertucci, Omar Motiño, Uxía Nogueira-Recalde, Mélanie Bourgin, Misha Mao, Yuhong Pan, Alexandra Cerone, Erwan Boedec, Zelia L. Gouveia, Federica Marmorino, Chiara Cremolini, Lisa Derosa, Laurence Zitvogel, Oliver Kepp, Carlos López-Otín, Maria Chiara Maiuri, Franck Perez, Philip Gorwood, Nicolas Ramoz, Franck Oury, Isabelle Martins, Guido Kroemer
Extracellular acyl-coenzyme A binding protein [ACBP encoded by diazepam binding inhibitor (DBI)] is a phylogenetically ancient appetite stimulator that is secreted in a nonconventional, autophagy-dependent fashion. Here, we show that low ACBP/DBI plasma concentrations are associated with poor prognosis in patients with anorexia nervosa, a frequent and often intractable eating disorder. In mice, anorexia induced by chronic restraint stress (CRS) is accompanied by a reduction in circulating ACBP/DBI concentrations. We engineered a chemical-genetic system for the secretion of ACBP/DBI through a biotin-activatable, autophagy-independent pathway. In transgenic mice expressing this system in hepatocytes, biotin-induced elevations in plasma ACBP/DBI concentrations prevented anorexia induced by CRS or chemotherapeutic agents including cisplatin, doxorubicin, and paclitaxel. ACBP/DBI reversed the CRS or cisplatin-induced increase in plasma lipocalin-2 concentrations and the hypothalamic activation of anorexigenic melanocortin 4 receptors, for which lipocalin-2 is an agonist. Daily intravenous injections of recombinant ACBP/DBI protein or subcutaneous implantation of osmotic pumps releasing recombinant ACBP/DBI mimicked the orexigenic effects of the chemical-genetic system. In conclusion, the supplementation of extracellular and peripheral ACBP/DBI might constitute a viable strategy for treating anorexia.
细胞外酰基辅酶 A 结合蛋白[ACBP,由地西泮结合抑制剂(DBI)编码]是一种系统发育古老的食欲刺激物,它以一种非常规的、依赖于自噬的方式分泌。在这里,我们发现低 ACBP/DBI 血浆浓度与神经性厌食症患者的不良预后有关,神经性厌食症是一种常见的、往往难以治愈的进食障碍。在小鼠中,慢性束缚应激(CRS)诱发的厌食症伴随着循环 ACBP/DBI 浓度的降低。我们设计了一个化学遗传系统,通过生物素激活的、不依赖自噬的途径分泌 ACBP/DBI。在肝细胞中表达该系统的转基因小鼠中,生物素诱导的血浆 ACBP/DBI 浓度升高可防止 CRS 或包括顺铂、多柔比星和紫杉醇在内的化疗药物诱导的厌食症。ACBP/DBI 逆转了 CRS 或顺铂诱导的血浆脂褐素-2 浓度的增加,以及下丘脑对厌食性黑皮素 4 受体的激活,而脂褐素-2 是黑皮素 4 受体的一种激动剂。每天静脉注射重组 ACBP/DBI 蛋白或皮下注射释放重组 ACBP/DBI 的渗透泵可模拟化学遗传系统的促厌食效应。总之,补充细胞外和外周 ACBP/DBI 可能是治疗厌食症的一种可行策略。
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Science Translational Medicine
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