Purpose: To study the damage and the expression of LC3 and p62 of condylar cartilage in fluorosis mouse.
Methods: Thirty 4-week-old male C57BL/6 mice were randomly divided into control group and the experimental group with 15 animals in each group. The control group received regular drinking water and the experimental group received a fluoride concentration of 75 mg/L drinking water for 8 weeks. The structure of condylar cartilage was observed through modified safranine O-fast green FCF cartilage stain kit. Immunohistochemistry was used to detect the expression of MMP-13, type Ⅱ collagen and LC3 and p62. Two-way analysis of variance test was conducted for analysis of semi-quantitative results of immunohistochemistry using SPSS 22.0 software package.
Results: Compared with the control group, the fibrocartilage layer of the experimental group became thinner, the condrocytes were smaller, and the staining became deeper.Immunohistochemistry results showed that the expression of MMP-13 and LC3 increased; the expression of type Ⅱ collagen and p62 decreased in the experimental group.
Conclusions: There was degeneration of the condylar cartilage and autophagy in mice with drinking water containing 75 mg/L fluoride.
{"title":"[Damage and changes in expression of autophagy related factors LC3 and p62 of condylar cartilage in fluorosis mouse].","authors":"Ying Yu, Qing-Qing Weng, Yin-Yue Luo, Shu-Ran Yao, Fang-Yu Yi, Ying Zhang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To study the damage and the expression of LC3 and p62 of condylar cartilage in fluorosis mouse.</p><p><strong>Methods: </strong>Thirty 4-week-old male C57BL/6 mice were randomly divided into control group and the experimental group with 15 animals in each group. The control group received regular drinking water and the experimental group received a fluoride concentration of 75 mg/L drinking water for 8 weeks. The structure of condylar cartilage was observed through modified safranine O-fast green FCF cartilage stain kit. Immunohistochemistry was used to detect the expression of MMP-13, type Ⅱ collagen and LC3 and p62. Two-way analysis of variance test was conducted for analysis of semi-quantitative results of immunohistochemistry using SPSS 22.0 software package.</p><p><strong>Results: </strong>Compared with the control group, the fibrocartilage layer of the experimental group became thinner, the condrocytes were smaller, and the staining became deeper.Immunohistochemistry results showed that the expression of MMP-13 and LC3 increased; the expression of type Ⅱ collagen and p62 decreased in the experimental group.</p><p><strong>Conclusions: </strong>There was degeneration of the condylar cartilage and autophagy in mice with drinking water containing 75 mg/L fluoride.</p>","PeriodicalId":21709,"journal":{"name":"Shanghai kou qiang yi xue = Shanghai journal of stomatology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141617113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose: To prepare single-atom iron nanocatalysts(SAF NCs) and explore its efficacy on oral squamous cell carcinoma Cal 27 cells in vitro, and provide a new strategy for the treatment of oral squamous cell carcinoma.
Methods: SAF NCs were prepared with combination of isolation and pyrolysis, the microscopic characterization was observed by transmission electron microscopy, the morphology and chemical composition were analysed by X-ray diffractograms and elemental distribution energy spectroscopy. The catalytic properties were detected by TMB assay and electron spin resonance test, and finally the changes in the activity of Cal27 cells were observed by CCK-8, flow cytometry and confocal microscopy for in vitro treatment of oral squamous carcinoma, to investigate the therapeutic effect against Cal27 cells. Statistical analysis was performed with GraphPad Prism 9 software package.
Results: SAF NCs were successfully synthesized and characterized, which showed excellent catalytic properties at the solution level and good biocompatibility in in vitro cellular level. The viability of Cal27 cell was reduce to 32.08% after in vitro catalytic treatment under conditions mimicking the characteristics of the tumor microenvironment.
Conclusions: Preliminary experiments demonstrated that SAF NCs with good biological properties could effectively inhibit the proliferation of Cal 27 cells in vitro, providing a new strategy for the treatment of oral squamous carcinoma.
目的:制备单原子铁纳米催化剂(SAF NCs),探讨其对口腔鳞状细胞癌Cal 27细胞的体外疗效,为口腔鳞状细胞癌的治疗提供新策略:方法:采用分离法和热解法制备 SAF NCs,透射电子显微镜观察其显微特征,X 射线衍射图和元素分布能谱分析其形态和化学成分。最后用 CCK-8、流式细胞仪和共聚焦显微镜观察了 Cal27 细胞活性的变化,并将其用于体外治疗口腔鳞癌,研究其对 Cal27 细胞的治疗效果。统计分析采用 GraphPad Prism 9 软件包:结果:成功合成并表征了 SAF NCs,其在溶液中表现出优异的催化性能,在体外细胞中表现出良好的生物相容性。在模拟肿瘤微环境特征的条件下,体外催化处理后 Cal27 细胞的存活率降至 32.08%:初步实验表明,具有良好生物特性的 SAF NCs 能有效抑制 Cal27 细胞的体外增殖,为治疗口腔鳞癌提供了一种新策略。
{"title":"[Nanocatalytic treatment of oral squamous cell carcinoma Cal 27 cells in vitro based on single-atom iron nanocatalysts].","authors":"Yu-Ting Xie, Ping Xiong","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To prepare single-atom iron nanocatalysts(SAF NCs) and explore its efficacy on oral squamous cell carcinoma Cal 27 cells in vitro, and provide a new strategy for the treatment of oral squamous cell carcinoma.</p><p><strong>Methods: </strong>SAF NCs were prepared with combination of isolation and pyrolysis, the microscopic characterization was observed by transmission electron microscopy, the morphology and chemical composition were analysed by X-ray diffractograms and elemental distribution energy spectroscopy. The catalytic properties were detected by TMB assay and electron spin resonance test, and finally the changes in the activity of Cal27 cells were observed by CCK-8, flow cytometry and confocal microscopy for in vitro treatment of oral squamous carcinoma, to investigate the therapeutic effect against Cal27 cells. Statistical analysis was performed with GraphPad Prism 9 software package.</p><p><strong>Results: </strong>SAF NCs were successfully synthesized and characterized, which showed excellent catalytic properties at the solution level and good biocompatibility in in vitro cellular level. The viability of Cal27 cell was reduce to 32.08% after in vitro catalytic treatment under conditions mimicking the characteristics of the tumor microenvironment.</p><p><strong>Conclusions: </strong>Preliminary experiments demonstrated that SAF NCs with good biological properties could effectively inhibit the proliferation of Cal 27 cells in vitro, providing a new strategy for the treatment of oral squamous carcinoma.</p>","PeriodicalId":21709,"journal":{"name":"Shanghai kou qiang yi xue = Shanghai journal of stomatology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141617122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose: To evaluation the effect of modified triangular flap-secondary healing (MTF-S) on the treatment of mandibular impacted wisdom teeth with full or partial bone impaction.
Methods: A total of 207 patients with mandibular impacted wisdom teeth were selected in Shaoxing Stomatological Hospital from June 2022 to June 2023. Among them, 86 patients had completely impacted wisdom teeth (group A), and 121 patients had partially impacted wisdom teeth (group B). All patients had bilateral impacted wisdom teeth. One of the wisdom teeth was removed first and was sutured with triangular flap-primary healing (TF-P). The other wisdom tooth was removed two weeks later and was sutured with MTF-S. Patients in groups A and B were divided into two subgroups based on suture methods, with TF-P used for group A1 and B1, and MTF-S used for groups A2 and B2. Perioperative indicators, including surgical time, root loss rate, and completeness of extraction sockets were recorded; Postoperative complications of four groups, including pain, swelling, and limited mouth opening were compared. SPSS 22.0 software package was used for statistical analysis.
Results: The surgical time of group A1, A2, B1 and B2 was (17.69±3.28), (18.22±3.06), (12.37±3.72) and (12.64±4.13) minutes, respectively. The surgical time of group A1 and A2 was significantly longer than that of group B1 and B2 (P<0.05). Seven days after surgery, the VAS scores of group A1, A2, B1 and B2 were (1.17±0.34), (0.93±0.29), (0.48±0.15) and (0.76±0.21), respectively. The VAS scores of group B1 and B2 were lower than those of group A1 and A2, and group A2 was lower than group A1 and B2 was higher than group B1 group(P<0.05). On the 1st day, 3rd day, and 7th day after surgery, the swelling degree in group A1 was greater than that in group B1, and the swelling degree in group B1 was greater than that in group A2 and B2(P<0.05); while the limitation of mouth opening mouth in group A2 and B2 was lower than that in group A1 and B1, and the limitation of opening mouth in group B2 was lower than that in group A2(P<0.05).
Conclusions: Compared with partially impacted wisdom teeth, the extraction of completely impacted wisdom teeth has a longer surgical time. For completely impacted wisdom teeth, MTF-S is beneficial for reducing postoperative pain, swelling and mouth opening limitations. For partially impacted wisdom teeth, MTF-S is beneficial for reducing postoperative swelling and mouth opening limitations, but the effect is not significant in reducing patient pain.
{"title":"[Evaluation of the effect of MTF-S on the removal of impacted mandibular wisdom teeth with complete or partial crown impaction].","authors":"Cheng Kang, Ting Zhu, Shan-Feng Luo, Yi-Yi Lou","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluation the effect of modified triangular flap-secondary healing (MTF-S) on the treatment of mandibular impacted wisdom teeth with full or partial bone impaction.</p><p><strong>Methods: </strong>A total of 207 patients with mandibular impacted wisdom teeth were selected in Shaoxing Stomatological Hospital from June 2022 to June 2023. Among them, 86 patients had completely impacted wisdom teeth (group A), and 121 patients had partially impacted wisdom teeth (group B). All patients had bilateral impacted wisdom teeth. One of the wisdom teeth was removed first and was sutured with triangular flap-primary healing (TF-P). The other wisdom tooth was removed two weeks later and was sutured with MTF-S. Patients in groups A and B were divided into two subgroups based on suture methods, with TF-P used for group A1 and B1, and MTF-S used for groups A2 and B2. Perioperative indicators, including surgical time, root loss rate, and completeness of extraction sockets were recorded; Postoperative complications of four groups, including pain, swelling, and limited mouth opening were compared. SPSS 22.0 software package was used for statistical analysis.</p><p><strong>Results: </strong>The surgical time of group A1, A2, B1 and B2 was (17.69±3.28), (18.22±3.06), (12.37±3.72) and (12.64±4.13) minutes, respectively. The surgical time of group A1 and A2 was significantly longer than that of group B1 and B2 (P<0.05). Seven days after surgery, the VAS scores of group A1, A2, B1 and B2 were (1.17±0.34), (0.93±0.29), (0.48±0.15) and (0.76±0.21), respectively. The VAS scores of group B1 and B2 were lower than those of group A1 and A2, and group A2 was lower than group A1 and B2 was higher than group B1 group(P<0.05). On the 1st day, 3rd day, and 7th day after surgery, the swelling degree in group A1 was greater than that in group B1, and the swelling degree in group B1 was greater than that in group A2 and B2(P<0.05); while the limitation of mouth opening mouth in group A2 and B2 was lower than that in group A1 and B1, and the limitation of opening mouth in group B2 was lower than that in group A2(P<0.05).</p><p><strong>Conclusions: </strong>Compared with partially impacted wisdom teeth, the extraction of completely impacted wisdom teeth has a longer surgical time. For completely impacted wisdom teeth, MTF-S is beneficial for reducing postoperative pain, swelling and mouth opening limitations. For partially impacted wisdom teeth, MTF-S is beneficial for reducing postoperative swelling and mouth opening limitations, but the effect is not significant in reducing patient pain.</p>","PeriodicalId":21709,"journal":{"name":"Shanghai kou qiang yi xue = Shanghai journal of stomatology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140860674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose: To explore the mechanism of SETDB1 inhibiting epithelial mesenchymal transition (EMT),migration and invasion in oral cancer via SOX 7 methylation.
Methods: SETDB1 and SOX7 mRNA and protein expression levels in KB cells of oral cancer and oral mucosal epithelial ATCC cells were determined by qRT-PCR and Western blot (WB). SETDB1 si-RNA was structured, then transfect into KB cells of oral cancer by liposome-mediated method. siRNA-SETDB1 was the experimental group (si-S), siRNA empty vector was the negative control group (si-N), and untransfected KB cells were the blank control group(NC). SETDB1 mRNA and protein expression levels were detected by qRT-PCR and Western blot(WB), to verify the transfection effect. The methylation levels of SOX7 were determined by pyrosequencing. The expression of N-cadherin, Vimentin, β-catenin, and Slug proteins was detected by WB. Cell viability was measured by MTT assay, migration ability was tested by scratch healing assay, and invasion ability was tested by Transwell chamber assay. Statistical analysis was performed with SPSS 21.0 software package.
Results: The results of Rt-qPCR and WB showed that the SETDB1 mRNA and protein expression decreased significantly in si-S group(P<0.05). Pyrosequencing test results showed that the regulation of SETDB1 could significantly reduce the SOX7 methylation rate and increased the SOX7 protein expression. WB results showed that knockdown of SETDB1 significantly inhibited the expression of EMT-related proteins N-cadherin, Vimentin, β-catenin and Slug in oral cancer KB cells (P<0.05). The results of cell functology experiments showed that knockdown of SETDB1 could significantly inhibit survival, migration and invasion of KB cells.
Conclusions: Downregulation of SETDB1 could suppress EMT, migration and invasion of oral cancer cells by regulating SOX7 methylation level, providing new ideas and targets for the diagnosis and treatment of oral cancer.
{"title":"[Downregulation of SETDB1 suppresses epithelial mesenchymal transition and invasion and metastasis in oral cancer via SOX7 methylation].","authors":"Yan Hao, Xiang-Yu Bai, Feng Huo, Xi-Bo Chen","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To explore the mechanism of SETDB1 inhibiting epithelial mesenchymal transition (EMT),migration and invasion in oral cancer via SOX 7 methylation.</p><p><strong>Methods: </strong>SETDB1 and SOX7 mRNA and protein expression levels in KB cells of oral cancer and oral mucosal epithelial ATCC cells were determined by qRT-PCR and Western blot (WB). SETDB1 si-RNA was structured, then transfect into KB cells of oral cancer by liposome-mediated method. siRNA-SETDB1 was the experimental group (si-S), siRNA empty vector was the negative control group (si-N), and untransfected KB cells were the blank control group(NC). SETDB1 mRNA and protein expression levels were detected by qRT-PCR and Western blot(WB), to verify the transfection effect. The methylation levels of SOX7 were determined by pyrosequencing. The expression of N-cadherin, Vimentin, β-catenin, and Slug proteins was detected by WB. Cell viability was measured by MTT assay, migration ability was tested by scratch healing assay, and invasion ability was tested by Transwell chamber assay. Statistical analysis was performed with SPSS 21.0 software package.</p><p><strong>Results: </strong>The results of Rt-qPCR and WB showed that the SETDB1 mRNA and protein expression decreased significantly in si-S group(P<0.05). Pyrosequencing test results showed that the regulation of SETDB1 could significantly reduce the SOX7 methylation rate and increased the SOX7 protein expression. WB results showed that knockdown of SETDB1 significantly inhibited the expression of EMT-related proteins N-cadherin, Vimentin, β-catenin and Slug in oral cancer KB cells (P<0.05). The results of cell functology experiments showed that knockdown of SETDB1 could significantly inhibit survival, migration and invasion of KB cells.</p><p><strong>Conclusions: </strong>Downregulation of SETDB1 could suppress EMT, migration and invasion of oral cancer cells by regulating SOX7 methylation level, providing new ideas and targets for the diagnosis and treatment of oral cancer.</p>","PeriodicalId":21709,"journal":{"name":"Shanghai kou qiang yi xue = Shanghai journal of stomatology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140857984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose: To compare the forming ability of four kinds of nickel-titanium instrument preparation resin for simulated curved root canal.
Methods: A total of 40 single bend resin simulated root canals were randomly divided into 4 groups with 10 in each group. Four kinds of nickel-titanium instruments (ProTaper, HyFlex EDM, WaveOne Gold and Reciproc Blue) were used for root canal preparation, and divided into group A, group B, group C and group D. The preparation time of the four groups was compared, the root canal images before and after preparation were analyzed by computer image analysis software, and the changes of the preparation time, curvature and curvature radius of the four groups were recorded. With the root tip as the center of the circle, the radius of 1-10 mm was selected as concentric circle arcs. The detection points were overlapping root canal intersection points. The resin removal amount and center positioning force of the inner and outer walls of the root canal at different detection points were recorded. Statistical analysis was performed with SPSS 20.0 software package.
Results: The root canal preparation time in group A was significant longer than that in group B, C and D(P<0.05), but there was no significant difference in the curvature and curvature radius of the root canal among the four groups (P>0.05). The removal amount of resin from the root canal wall in group C was significant lower than that in group A, B and D (P<0.05) when the distance from the detection point to the apical foramina was 5, 6, 8, 9 and 10 mm, respectively. The removal amount of resin from the outer wall of the root canal in group C was significant lower than that in group A, B and D (P<0.05) when the distance from the detection point to the apical foramina was 5, 6, 7, 9 and 10 mm, respectively. The root tip offset of group A from the detection point to the apical hole of 1, 2, 3, 4, 6, 7, 8, 9 and 10 mm was significant greater than that of group B, C and D(P<0.05).
Conclusions: Among the four instruments, ProTaper has the largest apical deviation, HyFlex EDM, WaveOne Gold and Reciproc Blue have better ability of root canal forming.
目的:比较四种镍钛器械预备树脂对模拟弯曲根管的成形能力:方法:将 40 个单弯树脂模拟根管随机分为 4 组,每组 10 个。比较四组的制备时间,用计算机图像分析软件分析制备前后的根管图像,记录四组的制备时间、弯曲度和弯曲半径的变化。以根尖为圆心,选取半径为 1-10 mm 的同心圆弧。检测点为根管交叉重叠点。记录不同检测点的树脂去除量和根管内外壁的中心定位力。使用 SPSS 20.0 软件包进行统计分析:A组的根管预备时间明显长于B、C、D组(P<0.05),但四组根管的弯曲度和弯曲半径无明显差异(P>0.05)。当检测点到根尖孔的距离分别为 5、6、8、9 和 10 mm 时,C 组的根管壁树脂去除量明显低于 A、B 和 D 组(P<0.05)。当检测点到根尖孔的距离分别为 5、6、7、9 和 10 毫米时,C 组根管外壁上树脂的去除量明显低于 A、B 和 D 组(P<0.05)。从检测点到根尖孔的距离分别为 1、2、3、4、6、7、8、9 和 10 mm 时,A 组的根尖偏移量明显大于 B、C 和 D 组(P<0.05):四种器械中,ProTaper 的根尖偏差最大,HyFlex EDM、WaveOne Gold 和 Reciproc Blue 的根管成形能力更强。
{"title":"[Comparison of the ability of four different kinds of nickel-titanium instruments to prepare simulated resin to simulate curved root canal forming].","authors":"Zhao-Huang Liang, Yao Zhang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To compare the forming ability of four kinds of nickel-titanium instrument preparation resin for simulated curved root canal.</p><p><strong>Methods: </strong>A total of 40 single bend resin simulated root canals were randomly divided into 4 groups with 10 in each group. Four kinds of nickel-titanium instruments (ProTaper, HyFlex EDM, WaveOne Gold and Reciproc Blue) were used for root canal preparation, and divided into group A, group B, group C and group D. The preparation time of the four groups was compared, the root canal images before and after preparation were analyzed by computer image analysis software, and the changes of the preparation time, curvature and curvature radius of the four groups were recorded. With the root tip as the center of the circle, the radius of 1-10 mm was selected as concentric circle arcs. The detection points were overlapping root canal intersection points. The resin removal amount and center positioning force of the inner and outer walls of the root canal at different detection points were recorded. Statistical analysis was performed with SPSS 20.0 software package.</p><p><strong>Results: </strong>The root canal preparation time in group A was significant longer than that in group B, C and D(P<0.05), but there was no significant difference in the curvature and curvature radius of the root canal among the four groups (P>0.05). The removal amount of resin from the root canal wall in group C was significant lower than that in group A, B and D (P<0.05) when the distance from the detection point to the apical foramina was 5, 6, 8, 9 and 10 mm, respectively. The removal amount of resin from the outer wall of the root canal in group C was significant lower than that in group A, B and D (P<0.05) when the distance from the detection point to the apical foramina was 5, 6, 7, 9 and 10 mm, respectively. The root tip offset of group A from the detection point to the apical hole of 1, 2, 3, 4, 6, 7, 8, 9 and 10 mm was significant greater than that of group B, C and D(P<0.05).</p><p><strong>Conclusions: </strong>Among the four instruments, ProTaper has the largest apical deviation, HyFlex EDM, WaveOne Gold and Reciproc Blue have better ability of root canal forming.</p>","PeriodicalId":21709,"journal":{"name":"Shanghai kou qiang yi xue = Shanghai journal of stomatology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140861625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose: To understand the current situation and needs of occlusal function exercise in patients with mandibular defects after oral tumor surgery. METHODS: Twenty-seven patients with mandibular defects after oral tumor surgery were interviewed semi-structurally by objective sampling method. The three-level coding system of rooting theory was used for reference, and Nvivo11 qualitative analysis software was used for bottom-up coding analysis.
Results: The current situation and needs of occlusal function exercise in patients with mandibular defects can be summarized into five themes, the impact of mandibular defects on patients, lack of knowledge related to occlusal function exercise, demand for content of occlusal function exercise (looking forward to professional health guidance), demand for education methods of occlusal function exercise(expecting diversified education methods and various obstacles to reduce patient compliance).
Conclusions: To establish a unified and standardized program of occlusal function exercise, doctors and nurses should pay attention to various obstacles of patients, adjust the psychological state of patients, and maximize the compliance of patients with occlusal function exercise.
{"title":"[A qualitative study on the needs of patients with mandibular defects for occlusal function].","authors":"Yan Zhao, Ying Zhang, Qian Chen, Li-Li Hou","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To understand the current situation and needs of occlusal function exercise in patients with mandibular defects after oral tumor surgery. METHODS: Twenty-seven patients with mandibular defects after oral tumor surgery were interviewed semi-structurally by objective sampling method. The three-level coding system of rooting theory was used for reference, and Nvivo11 qualitative analysis software was used for bottom-up coding analysis.</p><p><strong>Results: </strong>The current situation and needs of occlusal function exercise in patients with mandibular defects can be summarized into five themes, the impact of mandibular defects on patients, lack of knowledge related to occlusal function exercise, demand for content of occlusal function exercise (looking forward to professional health guidance), demand for education methods of occlusal function exercise(expecting diversified education methods and various obstacles to reduce patient compliance).</p><p><strong>Conclusions: </strong>To establish a unified and standardized program of occlusal function exercise, doctors and nurses should pay attention to various obstacles of patients, adjust the psychological state of patients, and maximize the compliance of patients with occlusal function exercise.</p>","PeriodicalId":21709,"journal":{"name":"Shanghai kou qiang yi xue = Shanghai journal of stomatology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140852718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose: To analyze the bone remodeling around the implant 10 years after disk-up sinus reamer(DSR)-based internal sinus floor elevation with implantation and to investigate the influence of different factors on implant retention.
Methods: The clinical and imaging data of patients undergoing DSR-based sinus floor elevation with simultaneous implantation were collected from the Department of Dental Implantology, Affiliated Hospital of Qingdao University from January 2008 to December 2011. Panoramic film and CBCT were used to measure the changes of bone mass around implant in different periods. Kaplan-Meier and Log-rank tests were used to analyze the effects of different factors on implant retention with SPSS 26.0 software package.
Results: The study included 98 patients with a total of 128 implants. During the follow-up of 0-168 months, 7 implants failed, and the remaining formed good osseointegration and functioned, with a 10-year cumulative retention rate of 94.53%. The height of bone formation was (0.29±0.15) mm at the top and (2.74±0.66) mm in the sinus of 75 implant sites with complete imaging data obtained ten years after surgery. Kaplan-Meier and Log-rank tests showed that 8 factors including initial bone height, elevated bone height, mucosal perforation, implant length, implant torsion, diabetes, smoking and periodontitis had significant effects on implant retention.
Conclusions: The DSR-based internal sinus floor elevation with implantation is a reliable and stable bone augmentation operation for vertical bone defect in maxillary posterior region, with a 10-year cumulative retention rate of no less than 94%. Initial bone height, elevated bone height, mucosal perforation, implant length, implant torsion, diabetes, smoking and periodontitis are the important factors affecting the long-term retention rate of implants.
{"title":"[Review of clinical effects of disk-up sinus reamer(DSR)-based internal sinus floor elevation with implantation in 10 years].","authors":"Kai-Yue Zhang, Ze-Yu Han, Shuai Jiang, Hao Xu, Fan Li, Bao-Dong Zhao","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To analyze the bone remodeling around the implant 10 years after disk-up sinus reamer(DSR)-based internal sinus floor elevation with implantation and to investigate the influence of different factors on implant retention.</p><p><strong>Methods: </strong>The clinical and imaging data of patients undergoing DSR-based sinus floor elevation with simultaneous implantation were collected from the Department of Dental Implantology, Affiliated Hospital of Qingdao University from January 2008 to December 2011. Panoramic film and CBCT were used to measure the changes of bone mass around implant in different periods. Kaplan-Meier and Log-rank tests were used to analyze the effects of different factors on implant retention with SPSS 26.0 software package.</p><p><strong>Results: </strong>The study included 98 patients with a total of 128 implants. During the follow-up of 0-168 months, 7 implants failed, and the remaining formed good osseointegration and functioned, with a 10-year cumulative retention rate of 94.53%. The height of bone formation was (0.29±0.15) mm at the top and (2.74±0.66) mm in the sinus of 75 implant sites with complete imaging data obtained ten years after surgery. Kaplan-Meier and Log-rank tests showed that 8 factors including initial bone height, elevated bone height, mucosal perforation, implant length, implant torsion, diabetes, smoking and periodontitis had significant effects on implant retention.</p><p><strong>Conclusions: </strong>The DSR-based internal sinus floor elevation with implantation is a reliable and stable bone augmentation operation for vertical bone defect in maxillary posterior region, with a 10-year cumulative retention rate of no less than 94%. Initial bone height, elevated bone height, mucosal perforation, implant length, implant torsion, diabetes, smoking and periodontitis are the important factors affecting the long-term retention rate of implants.</p>","PeriodicalId":21709,"journal":{"name":"Shanghai kou qiang yi xue = Shanghai journal of stomatology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140866261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Juan-Juan Su, Hong-Ting Chen, Xu Wang, Qiang Jin, Lin Wang
Purpose: To study the relationship between the expression of prostaglandin E2 (PGE2) and cyclooxygenase-2 (COX-2) and the osteogenic activity and oxygen level of alveolar bone.
Methods: The alveolar bones of 56 patients with chronic periodontitis who received dental treatment from March 2021 to March 2023 were collected as the experimental (periodontitis) group, and the healthy alveolar bones of 53 patients who received dental treatment during the same period were selected as the control group. The osteoblasts were cultured by tissue block culture, and modified Kaplow's alkaline phosphatase (ALP) staining was used to identify the cells. COX-2, PGE2 and osteoclastogenesis inhibitory factor (OPG) receptor activator of nuclear factor-κb ligand (RANKL) and other indicators were determined by ELISA. PGE2, COX-2, OPG, internal oxygen level, ALP, RANKL and their correlation were compared between the two groups. Statistical analysis was performed with SPSS 27.0 software package.
Results: PGE2, COX-2 and RANKL in periodontitis group were significantly higher than those in the control group, but OPG, internal oxygen level and ALP were significantly lower than those in the control group (P<0.05). PGE2 and COX2 were highly positively correlated with OPG, internal oxygen level and ALP, but were highly positively correlated with RANKL(P<0.05).
Conclusions: The expression of PGE2 and COX-2 is highly negatively correlated with ALP and oxygen levels. Clinical treatment may consider increasing oxygen levels, increasing oxygen partial pressure, and regulating ALP levels by drugs, so as to change the inflammatory condition of periodontitis or other dental diseases.
{"title":"[The relationship between the expression of PGE2 and COX-2 and the osteogenic activity and oxygen level of alveolar bone].","authors":"Juan-Juan Su, Hong-Ting Chen, Xu Wang, Qiang Jin, Lin Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To study the relationship between the expression of prostaglandin E2 (PGE2) and cyclooxygenase-2 (COX-2) and the osteogenic activity and oxygen level of alveolar bone.</p><p><strong>Methods: </strong>The alveolar bones of 56 patients with chronic periodontitis who received dental treatment from March 2021 to March 2023 were collected as the experimental (periodontitis) group, and the healthy alveolar bones of 53 patients who received dental treatment during the same period were selected as the control group. The osteoblasts were cultured by tissue block culture, and modified Kaplow's alkaline phosphatase (ALP) staining was used to identify the cells. COX-2, PGE2 and osteoclastogenesis inhibitory factor (OPG) receptor activator of nuclear factor-κb ligand (RANKL) and other indicators were determined by ELISA. PGE2, COX-2, OPG, internal oxygen level, ALP, RANKL and their correlation were compared between the two groups. Statistical analysis was performed with SPSS 27.0 software package.</p><p><strong>Results: </strong>PGE2, COX-2 and RANKL in periodontitis group were significantly higher than those in the control group, but OPG, internal oxygen level and ALP were significantly lower than those in the control group (P<0.05). PGE2 and COX2 were highly positively correlated with OPG, internal oxygen level and ALP, but were highly positively correlated with RANKL(P<0.05).</p><p><strong>Conclusions: </strong>The expression of PGE2 and COX-2 is highly negatively correlated with ALP and oxygen levels. Clinical treatment may consider increasing oxygen levels, increasing oxygen partial pressure, and regulating ALP levels by drugs, so as to change the inflammatory condition of periodontitis or other dental diseases.</p>","PeriodicalId":21709,"journal":{"name":"Shanghai kou qiang yi xue = Shanghai journal of stomatology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140865203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shan Zhao, Lin Zhang, Sheng-Nan Li, Nan Kang, Jian Meng, Xiao-Dong Li
Purpose: Bioactive magnesium ions were successfully incorporated into the nanoporous titanium base coating by micro-arc oxidation(MAO), and its physical properties and osteogenic effects were explored.
Methods: Non-magnesium-containing and magnesium-containing titanium porous titanium coatings(MAO, MAO-mg) were prepared by changing the composition of MAO electrolyte and controlling the doping of magnesium in porous titanium coatings. The samples were characterized by scanning electron microscope (SEM), roughness, contact angle and energy dispersive X-ray spectrometer (EDS). Mg2+ release ability of magnesium-doped nanoporous titanium coatings was determined by inductively coupled plasma/optical emission spectrometer(ICP-OES). The structure of the cytoskeleton was determined by live/dead double staining, CCK-8 detection of material proliferation-toxicity, and staining of β-actin using FITC-phalloidin. The effects of the coating on osteogenic differentiation in vitro were determined by alizarin red (ARS), alkaline phosphatase (ALP) staining and real-time polymerase chain reaction (qRT-PCR). SPSS 25.0 software package was used for statistical analysis.
Results: The MAO electrolyte with magnesium ions did not change the surface characteristics of the porous titanium coating. Each group prepared by MAO had similar microporous structure(P>0.05). There was no significant difference in surface roughness and contact angle between MAO treatment group (MAO, MAO-mg)(P>0.05), but significantly higher than that of Ti group (P<0.05). With the passage of cell culture time, MAO-mg group promoted cell proliferation (P<0.05). MAO-mg group was significantly higher than other groups in ALP and ARS staining. The expression of Runx2 mRNA (P<0.05), ALP(P<0.05) and osteocalcin OCN(P<0.05) in MAO-mg group was significantly higher than that in Ti and MAO groups.
Conclusions: MAO successfully prepared magnesium-containing nanoporous titanium coating, and showed a significant role in promoting osteogenic differentiation.
目的:通过微弧氧化(MAO)将生物活性镁离子成功地加入到纳米多孔钛基涂层中,并探讨其物理性质和成骨作用:方法:通过改变MAO电解液的成分和控制镁在多孔钛涂层中的掺杂量,制备了不含镁和含镁的多孔钛涂层(MAO,MAO-mg)。样品用扫描电子显微镜(SEM)、粗糙度、接触角和能量色散 X 射线光谱仪(EDS)进行了表征。电感耦合等离子体/光发射光谱(ICP-OES)测定了掺镁纳米多孔钛涂层的 Mg2+ 释放能力。通过活/死双重染色、CCK-8检测材料的增殖-毒性以及用FITC-类花青素染色β-肌动蛋白,测定了细胞骨架的结构。茜素红(ARS)、碱性磷酸酶(ALP)染色和实时聚合酶链反应(qRT-PCR)测定了涂层对体外成骨分化的影响。统计分析采用 SPSS 25.0 软件包:含镁离子的 MAO 电解质没有改变多孔钛涂层的表面特性。各组 MAO 制备的微孔结构相似(P>0.05)。MAO处理组(MAO、MAO-mg)的表面粗糙度和接触角无明显差异(P>0.05),但明显高于Ti组(P<0.05)。随着细胞培养时间的延长,MAO-mg 组促进细胞增殖(P<0.05)。MAO-mg组的ALP和ARS染色明显高于其他组。MAO-mg组Runx2 mRNA(P<0.05)、ALP(P<0.05)和骨钙素OCN(P<0.05)的表达均明显高于Ti组和MAO组:结论:MAO成功制备了含镁纳米多孔钛涂层,并在促进成骨分化方面发挥了重要作用。
{"title":"[Biological characteristics and osteogenic differentiation of magnesium-doped nanoporous titanium coating].","authors":"Shan Zhao, Lin Zhang, Sheng-Nan Li, Nan Kang, Jian Meng, Xiao-Dong Li","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>Bioactive magnesium ions were successfully incorporated into the nanoporous titanium base coating by micro-arc oxidation(MAO), and its physical properties and osteogenic effects were explored.</p><p><strong>Methods: </strong>Non-magnesium-containing and magnesium-containing titanium porous titanium coatings(MAO, MAO-mg) were prepared by changing the composition of MAO electrolyte and controlling the doping of magnesium in porous titanium coatings. The samples were characterized by scanning electron microscope (SEM), roughness, contact angle and energy dispersive X-ray spectrometer (EDS). Mg<sup>2+</sup> release ability of magnesium-doped nanoporous titanium coatings was determined by inductively coupled plasma/optical emission spectrometer(ICP-OES). The structure of the cytoskeleton was determined by live/dead double staining, CCK-8 detection of material proliferation-toxicity, and staining of β-actin using FITC-phalloidin. The effects of the coating on osteogenic differentiation in vitro were determined by alizarin red (ARS), alkaline phosphatase (ALP) staining and real-time polymerase chain reaction (qRT-PCR). SPSS 25.0 software package was used for statistical analysis.</p><p><strong>Results: </strong>The MAO electrolyte with magnesium ions did not change the surface characteristics of the porous titanium coating. Each group prepared by MAO had similar microporous structure(P>0.05). There was no significant difference in surface roughness and contact angle between MAO treatment group (MAO, MAO-mg)(P>0.05), but significantly higher than that of Ti group (P<0.05). With the passage of cell culture time, MAO-mg group promoted cell proliferation (P<0.05). MAO-mg group was significantly higher than other groups in ALP and ARS staining. The expression of Runx2 mRNA (P<0.05), ALP(P<0.05) and osteocalcin OCN(P<0.05) in MAO-mg group was significantly higher than that in Ti and MAO groups.</p><p><strong>Conclusions: </strong>MAO successfully prepared magnesium-containing nanoporous titanium coating, and showed a significant role in promoting osteogenic differentiation.</p>","PeriodicalId":21709,"journal":{"name":"Shanghai kou qiang yi xue = Shanghai journal of stomatology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140868435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ying Fang, Wen-Yan Huang, Guo-Hou Miao, Si Meng, Bo Peng, Su-Juan Zeng
Purpose: To study the antimicrobial effect of different concentrations of new bioactive glass(BG) on common bacteria in apical periodontitis of deciduous teeth.
Methods: The diameter (mm) of the inhibitory rings formed after treatment of Enterococcus faecalis, Porphyromonas gingivalis and Clostridium nucleatum with the new bioactive glass was detected and observed by paper diffusion method, and the minimal inhibitory concentration(MIC), minimal bactericidal concentration (MBC) and minimal biofilm eradication concentration (MBEC) of E. faecalis, P. gingivalis and C. pseudomallei were determined. The mixed plaques of the three bacteria were treated with 20, 40, 60 and 80 mg/mL of the new bioactive glass for 24 h. The results were analyzed by laser confocal microscopy. The antibacterial effect of the new bioactive glass on the mixed plaque was observed by confocal laser scanning microscopy (CLSM). Statistical analysis was performed with GraphPad Prism 10.0 software.
Results: The new bioactive glass showed strong antibacterial potential against the common bacteria of apical periodontitis; the MBEC of the new bioactive glass on the plaque was significantly greater than MIC and MBC of Enterococcus faecalis, Porphyromonas gingivalis and Clostridium nucleatum, and as the concentration of the new bioactive glass increased, the number of dead bacteria in the mixed plaque increased, and there was significant difference from that of the blank control group (P<0.05).
Conclusions: The novel bioactive glass shows significant antibacterial efficacy against Enterococcus faecalis, Porphyromonas gingivalis and Clostridium nucleatum, which are the common bacteria in apical periodontitis of deciduous teeth.
{"title":"[Study on the antimicrobial effect of new bioactive glass against common bacteria in apical periodontitis of deciduous teeth].","authors":"Ying Fang, Wen-Yan Huang, Guo-Hou Miao, Si Meng, Bo Peng, Su-Juan Zeng","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To study the antimicrobial effect of different concentrations of new bioactive glass(BG) on common bacteria in apical periodontitis of deciduous teeth.</p><p><strong>Methods: </strong>The diameter (mm) of the inhibitory rings formed after treatment of Enterococcus faecalis, Porphyromonas gingivalis and Clostridium nucleatum with the new bioactive glass was detected and observed by paper diffusion method, and the minimal inhibitory concentration(MIC), minimal bactericidal concentration (MBC) and minimal biofilm eradication concentration (MBEC) of E. faecalis, P. gingivalis and C. pseudomallei were determined. The mixed plaques of the three bacteria were treated with 20, 40, 60 and 80 mg/mL of the new bioactive glass for 24 h. The results were analyzed by laser confocal microscopy. The antibacterial effect of the new bioactive glass on the mixed plaque was observed by confocal laser scanning microscopy (CLSM). Statistical analysis was performed with GraphPad Prism 10.0 software.</p><p><strong>Results: </strong>The new bioactive glass showed strong antibacterial potential against the common bacteria of apical periodontitis; the MBEC of the new bioactive glass on the plaque was significantly greater than MIC and MBC of Enterococcus faecalis, Porphyromonas gingivalis and Clostridium nucleatum, and as the concentration of the new bioactive glass increased, the number of dead bacteria in the mixed plaque increased, and there was significant difference from that of the blank control group (P<0.05).</p><p><strong>Conclusions: </strong>The novel bioactive glass shows significant antibacterial efficacy against Enterococcus faecalis, Porphyromonas gingivalis and Clostridium nucleatum, which are the common bacteria in apical periodontitis of deciduous teeth.</p>","PeriodicalId":21709,"journal":{"name":"Shanghai kou qiang yi xue = Shanghai journal of stomatology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140852740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}