上海口腔医学最新文献

Purpose: To explore the molecular mechanism of circ_0000326 regulating proliferation, invasion and migration of oral squamous cell carcinoma HSC3 cells.

Methods: Cancerous tissue and adjacent tissue specimens of 45 patients with oral squamous cell carcinoma (OSCC) admitted to the Second People's Hospital of Hefei from March 2020 to June 2021 were collected. qRT-PCR was used to detect the expression levels of circ_0000326 and miR-567. CCK-8, plate clone formation test, scratch test and Transwell test were used to detect cell proliferation, clone formation, migration and invasion. Dual luciferase reporter experiment was used to detect the targeting relationship between circ_0000326 and miR-567. Western blot was used to quantify E-cadherin and N-cadherin protein. SPSS 21.0 software package was used for statistical analysis.

Results: circ_0000326 expression was 4.01±0.29 in OSCC and 1.00±0.13 in paracancerous tissues, while miR-567 expression was 0.28±0.03 and 1.00±0.10, respectively, with significant differences. Compared with the si-NC group, the cell viability and the number of cell clones in the si-circ_0000326 group were significantly decreased(P＜0.05). Compared with the si-NC group, the number of invasive cells and scratch healing rate in the si-circ_0000326 group were significantly decreased (P＜0.05), the expression level of E-cadherin protein was significantly increased (P＜0.05), and the expression level of N-cadherin protein was significantly decreased(P＜0.05). Additionally, circ_0000326 targeted miR-567. miR-567 expression was 1.00±0.00 in pcDNA group, 0.44±0.04 in pcDNA-circ_0000326 group, 0.99±0.06 in si-NC group, and 2.92±0.25 in si-circ_0000326 group with significant differences. Compared with miR-NC group, the cell viability, scratch healing rate, the number of cell clones and the number of invasive cells of miR-567 group were decreased, while E-cadherin protein level was increased(P＜0.05). Compared with si-circ_0000326+anti-miR-NC group, the cell viability, scratch healing rate, N-cadherin protein level, the number of cell clones and the number of invasive cells of si-circ_0000326+anti-miR-567 group were increased(P＜0.05), while E-cadherin protein level was decreased(P＜0.05).

Conclusions: Interference with the expression of circ_0000326 could reduce the ability of OSCC cell proliferation, colony formation, migration and invasion by promoting the expression of miR-567.

Purpose: The potential relationship between impacted mandibular third molar and lingual bone thickness was investigated by quantitative measurement of lingual bone thickness, and a three-dimensional visualization model of lingual bone was established.

Methods: Image data of 200 cases of mandibular impacted third molar were collected from the database of Hefei Stomatological Hospital. Thickness measurement and three-dimensional reconstruction of lingual bone at different measurement sites were performed by Simplant Pro software. Statistical analysis was performed using SPSS 22.0 software package, and the comparison of lingual thickness at different measurement sites was performed using rank sum test. Whether the thickness of bone plate was "high-risk type" was taken as the result variable, different related factors were analyzed by logistic regression.

Results: The lingual bone at the middle point of the root of the third molar was the thinnest. Multiple teeth, mesio-inclined and dial-inclined teeth, and Class Ⅱ and Ⅲ impacted teeth had thin lingual bone(P＜0.05). The mesial and distal inclines were observed, and the lingual bone was thin in the root apical region of the teeth in Class Ⅱ and Class Ⅲ(P＜0.05). In the lower impacted teeth, the thin lingual bone at the central crown site was more likely to be found in Class Ⅲ teeth(P＜0.05).

Conclusions: The thickness of lingual bone was related to the number of impacted tooth roots, tilt direction and impacted type. In the extraction of impacted teeth, the above factors should be considered, and the injury of lingual nerve, lingual bone and surrounding soft tissue should be vigilant.

Purpose: To explore the effect of thick and thin sputum on the restoration of oral anterior teeth and the index of gingival crevicular fluid.

Methods: A study was conducted on 80 patients (130 teeth) who underwent full-ceramic crown treatment for anterior tooth restoration in Jiangnan University Affiliated Hospital 's oral clinic. The patients were divided into thick gingival type (42 cases, 67 teeth) and thin gingival type (38 cases, 63 teeth) according to their gingival biotype. The two groups were compared before and after the restoration with improved plaque index (mPI), modified bleeding index (mBI), probing depth (PD), clinical evaluation score of oral prosthesis, tumor necrosis factor-alpha (TNF-alpha) and IL-1 β(interleukin-1β) in gingival crevicular fluid. Statistical analysis was performed with SPSS 21.0 software package.

Results: Before treament, there were no significant differences in mPI, mBI and PD between the two groups (P＞0.05); 12 months after treament, mPI, mBI and PD of the thin gingiva group were significantly lower than those of the thick gingiva group(P＜0.05). Before treament, there were no significant differences in TNF-α and IL-1β levels in gingival crevicular fluid between the two groups(P＞0.05); 12 months after treament, TNF-α and IL-1β levels of the thin gingiva group were significantly lower than those of the thick gingiva group(P＜0.05). Six months after treament, there were no significant differences in prosthesis integrity, marginal adaptation, color matching, periodontal health status and secondary caries scores between the two groups (P＞0.05); 12 months after treament, the marginal adaptation, periodontal health status and secondary caries scores of the thick gingiva group were significantly higher than those of the thin gingiva group(P＜0.05); the marginal discoloration score of the thick gingiva group was significantly lower than that of the thin gingiva group at 6 months and 12 months after treament(P＜0.05).

Convlusions: The thin sputum type is more conducive to repairing the oral cavity after treatment, and is beneficial to the maintenance of oral health condition after repair. The gingival index of the thick sputum type patient will be significantly increased after repair, and the risk of gingival inflammation is increased. The aesthetic effect is better than the thin sputum type.

Purpose: To investigate the expression and significance of NEK2 and EMT-related molecules in oral squamous cell carcinoma (OSCC).

Methods: The expression levels of NEK2 and EMT-related molecules (E-Cadherin, N-Cadherin, Vimentin) in 60 cases of primary OSCC tissues and normal oral mucosa tissues were detected by immunohistochemistry(IHC). NEK2 mRNA expression in 25 OSCC tissues and 10 normal oral mucosa tissues was detected by qRT-PCR. Statistical analysis was performed using SPSS 26.0 software package.

Results: The expression of NEK2, N-Cadherin, and Vimentin increased in OSCC tissues, while the expression of E-Cadherin decreased(P＜0.05). The worse the differentiation, the higher the expression of NEK2 and the lower the expression of E-Cadherin(P＜0.05).

Conclusions: NEK2 can be used as a prognostic marker for OSCC. The up-regulation of NEK2 and the changes in the expression levels of EMT related molecules can promote the occurrence and development of OSCC.

Purpose: To investigate the potential mechanisms of scutellarin on oral leukoplakia (OLK) by network pharmacology and further verify by cytology.

Methods: The potential targets of scutellarin acting on OLK were excavated through network pharmacology. PPI network was constructed, and the possible targets and pathways of scutellarin were predicted by GO and KEGG enrichment analysis. CCK-8 and Transwell assays were used to verify the effects of scutellarin on proliferation, migration and invasion of Leuk-1 and Cal-27 cell lines. The expression of related molecules was detected by Western blot to explore potential molecular mechanisms. Statistical analysis was performed with GraphPad Prism 9 software package.

Results: There were 29 potential targets of scutellarin acting on OLK, of which HIF-1α was the key target, and the results of GO and KEGG analysis showed that scutellarin was highly involved in the response of cells and tissues to hypoxia and influenced HIF-1 signaling pathway. Scutellarin can significantly inhibit the proliferation (IC₅₀:2 mmol/L), invasion and migration of Leuk-1 and Cal-27 cells(P＜0.05), and downregulated the expression of HIF-1α in Leuk-1 and Cal-27 cells.

Conclusions: Scutellarin can inhibit carcinogenesis of OLK by suppressing HIF-1 signaling pathway.

Purpose: To investigate the feasibility and safety of the deep circumflex iliac artery (DCIA) derived chimeric flap through the anatomical study of the blood vessels and perforating branches in the ilioinguinal region, and to provide the basis for selecting different DCIA chimeric flap schemes according to the difficulty of surgery, defect conditions and repair needs.

Methods: Six Chinese adult specimens were dissected by retrograde perfusion of red latex into bilateral femoral arteries. At the same time, the length, diameter and main branch position of DCIA vascular pedicle were measured in 12 lower limb CTAs, and compared with the anatomical data. Six patients with oral tumors accompanied by mandibular defects who were treated in the Department of Oral and Maxillofacial Surgery, Affiliated Stomatological Hospital of Nanjing Medical University from July 2020 to November 2021 were repaired and reconstructed with the chimeric iliac myofascial flap. The postoperative appearance and occlusal function of the recipient area were observed. SPSS 19.0 software package was used for data analysis.

Results: A total of 19 DCIA perforators with an external diameter of ≥ 0.5 mm were found in 12 specimens of ilioinguinal region. These perforators were distributed in the 5 cm×3 cm area, inside the ilium and 5cm behind the anterior superior iliac spine. The length of DCIA vascular pedicle was (6.73±1.06) cm. The measured value of the external diameter of the starting position of the vascular pedicle was (2.55±0.29) mm. The outer diameter of DCIA skin perforator penetrating deep fascia was (1.12±0.14) mm. In the CTA analysis of 12 lower limbs, it was found that the length of DCIA vascular pedicle was (6.98±0.62) cm. The measured diameter at the original position of vascular pedicle was (2.35±0.20) mm. Six cases of mandibular defects were repaired with iliac internal oblique fascia mosaic flap. Six cases of lliac flap survived successfully after operation. Follow up for 6 to 24 months (average 12 months) showed that the mandibular shape and function recovered well, the intraoral myofascial flap became mucosal, and the implanted iliac bone showed no significant volume change on CT after operation. Walking and weight bearing in donor area were basically normal, and no abdominal hernia occurred.

Conclusions: DCIA and its main branches have a relatively constant course and distribution in the ilioinguinal region. According to the conditions of different defect areas, different tissue types of chimeric flaps based on DCIA can be prepared to meet the repair requirements. The donor site complications can be controlled, and it is an ideal choice to repair mandibular defects.

Purpose: To evaluate the efficacy of combination of deproteinized bovine bone matrix and concentrated growth factors in maintaining the three-dimensional contour of alveolar bone in ridge preservation of teeth with severe periodontitis.

Methods: Thirty patients with posterior teeth suffering from severe periodontitis requiring extraction and with the intention of implant restoration were selected and randomly divided into the experimental group and control group, with 15 cases in each group. In the experimental group, DBBM combined with CGF fluid was used as bone graft material and placed in the extraction socket of the patient after minimally invasive tooth extraction and thorough debridement,while DBBM was used in the control group mixed with normal saline as the bone graft material.The extraction wounds of both groups were covered with absorbable biofilm and free gingival tissue. CBCT was performed at the initial diagnosis, immediately after operation, and 6 months after operation, and the CT images were imported into Mimics 20.0 software package to measure the difference after fitting to obtain data, SPSS 26.0 software package was used for statistical analysis of the data.

Results: The vertical height of the alveolar bone in the experimental group and the control group was significantly increased 6 months after operation compared with the initial diagnosis (P＜0.05), but there was no significant difference between the 2 groups(P＞0.05). There was significant difference in the change of the alveolar bone width at 1 mm below the alveolar crest between the two groups at the initial diagnosis and 6 months after operation(P＜0.05), and the horizontal width absorption in the experimental group was smaller than that in the control group. The horizontal absorption rate of bone graft material in the two groups at 1 mm below the alveolar crest showed that the experimental group was lower than the control group, and the difference was statistically significant(P＜0.05).

Conclusions: Compared with DBBM alone, combined application of DBBM and CGF can better maintain the alveolar bone contour of the extraction socket with severe periodontitis.

Purpose: To study the relationship between the stylomastoid foramen and surrounding bony structures, enrich anatomical data and provide reference for clinical surgery.

Methods: A total of 62 intact and dry adult skulls were selected. The shape of the stylomastoid foramen was observed, the diameter of the stylomastoid foramen, the distances from the posterolateral point and the anterior medial point to the surrounding bony structures were measured with a vernier caliper. SPSS 25.0 software package was used to analyze the data.

Results: There were four shapes of stylomastoid foramen, i.e., circular (61.29%), oval (29.84%), irregular (8.06%) and triangular (0.81%). The circular diameter was (2.80±0.61) mm, the oval long and short diameters were (4.43±0.96) and (2.79±0.60) mm. Distances from the posterolateral and anterior medial points of the stylomastoid foramen to the posterolateral point of the external opening of the carotid canal, the anterior medial point of the jugular foramen, the midline, the most anterior point of the foramen magnum, the posterior point of the great palatine foramen, the posterolateral point of the foramen lacerated, the foramen ovale, the posterolateral point of the foramen spinosum, the anterior point of the styloid process root, the outermost point of the tympanomastoid fissure and the tip of the mastoid process were (16.10±2.81), (24.01±2.65), (44.95±3.24), (45.10±2.71), (61.66±4.14), (35.56±4.35), (32.26±2.85), (29.12±3.40), (10.39±3.25), (9.49±2.24) and (12.01±2.79) mm; (12.80±2.41), (21.56±2.51), (42.96±3.97), (42.91±2.76), (58.97±3.97), (32.98±4.14), (29.20±2.77), (25.80±2.87), (7.37±2.33), (11.42±2.00) and (15.41±2.57) mm, respectively. Statistical analysis showed that there was no significant difference in the apertures and distances between the left and right side(P＞0.05).

Conclusions: Most of the stylomastoid foramen are round and oval, understanding the distance between the foramen and surrounding bony structures is helpful for guiding clinical operations and enriching anatomical knowledge.

Purpose: To investigate the effectiveness of combined orthodontic and restoration treatment for introverted deep overbite patients with severe wear.

Methods: A total of 86 introverted deep overbite patients with severe wear admitted to Cangzhou People's Hospital from December 2020 to June 2022 were collected and divided into the control group and the experimental group by gender, age, degree of wear and tear, with 43 cases in each group. The control group received orthodontic treatment, while the experimental group received combined orthodontic and restoration treatment. The gingival index (GI), periodontal index(PI), smile index, temporomandibular joint space [anterior space, superior space, posterior space and In (P/A)], tooth function (chewing function, pronunciation function, dental occlusal function) and chewing efficiency of 2 groups before and after treatment were compared. Statistical analysis was performed with SPSS 22.0 software package.

Results: Compared with the control group, the orthodontic treatment time of the experimental group patients was significantly reduced(P＜0.05). Before treatment, there was no significant difference in GI, PI, smile index, buccal gap rate, temporomandibular joint space, swallowing function and chewing efficiency between 2 groups(P＞0.05). After treatment, compared with the control group, the GI, PI, anterior space were significantly reduced(P＜0.05); the smile index, posterior space and In (P/A), chewing function, pronunciation function, tooth occlusion and chewing efficiency significantly increased (P＜0.05).

Conclusions: Both orthodontic treatment and combined orthodontic and restoration treatment can be used to treat introverted deep overbite patients with severe wear. However, combination of orthodontic and restoration treatment has significant advantages.

Purpose: To investigate the current status of oral health literacy among adult orthodontic patients and analyze its influencing factors.

Methods: Totally 163 adult orthodontic patients were recruited by convenience sampling method from November 2021 to February 2022 at the Department of Oral and Craniomaxillofacial Surgery, Center of Craniofacial Orthodontics in Shanghai Ninth People's Hospital. General information questionnaire, Chinese version of Health Literacy in Dentistry (HeLD-14), Chinese version of Self-Efficacy Scale for Self-care (SESS) and Social Support Rating Scale (SSRS) were applied. SPSS 21.0 software package was used for data analysis.

Results: The mean score of oral health literacy in adult orthodontic patients was (48.74±5.54), and the lowest score were obtained in concern (6.80±1.19) and support (6.40±1.59) dimensions. Multiple stepwise regression analysis showed that adult orthodontic patients' consultation self-efficacy, social support utilization, family or friends' orthodontic history and objective support were the influencing factors of oral health literacy.

Conclusions: The oral health literacy in adult orthodontic patients is at a middle to high level, but concern and support literacy need to be improved. Strengthening consultation self-efficacy, objective support and utilization of social support can improve adult orthodontic patients' oral health literacy. Attention should be paid to patients who have no relatives or friends with history of orthodontic treatment.