This quasi-experimental study aimed to design and compare outcomes of two early experiential activities through community-based learning. Seventy pharmacy students were assigned into an intervention and control group to practice at certain communities. The program in the intervention group included a 1-day brief lecture on a basic physical examination and patient interview techniques and a 10-day program of four health-related activities; home visits, health screening, health education, and case discussion. The control group practiced a 10-day routine activity of the community. The students’ knowledge and attitude, the pharmacist and the practitioners’ attitude were assessed at the end of the program. The findings revealed that both groups showed improvement in total knowledge scores but basic health care score was significantly higher in the intervention group than in the control group (p<0.01). All students were satisfied with the program and responded that their knowledge was strengthened. From the pharmacists’ viewpoint, patient care experience encouraged students to take care of others and the program helped students to understand health and drug use problems in the community. To sum up, the program was able to strengthen the patient care knowledge of students and health concern of the community. These benefits will encourage both students and communities to further solve health problems.
{"title":"Strengthening Pharmacy Students’ Knowledge in Patient Care through Community-based Outreach Activities","authors":"Wiwat Thavornwattanayong, N. Kapol","doi":"10.14456/SUSTJ.2016.9","DOIUrl":"https://doi.org/10.14456/SUSTJ.2016.9","url":null,"abstract":"This quasi-experimental study aimed to design and compare outcomes of two early experiential activities through community-based learning. Seventy pharmacy students were assigned into an intervention and control group to practice at certain communities. The program in the intervention group included a 1-day brief lecture on a basic physical examination and patient interview techniques and a 10-day program of four health-related activities; home visits, health screening, health education, and case discussion. The control group practiced a 10-day routine activity of the community. The students’ knowledge and attitude, the pharmacist and the practitioners’ attitude were assessed at the end of the program. The findings revealed that both groups showed improvement in total knowledge scores but basic health care score was significantly higher in the intervention group than in the control group (p<0.01). All students were satisfied with the program and responded that their knowledge was strengthened. From the pharmacists’ viewpoint, patient care experience encouraged students to take care of others and the program helped students to understand health and drug use problems in the community. To sum up, the program was able to strengthen the patient care knowledge of students and health concern of the community. These benefits will encourage both students and communities to further solve health problems.","PeriodicalId":22107,"journal":{"name":"Silpakorn University Science and Technology Journal","volume":"28 1","pages":"20-28"},"PeriodicalIF":0.0,"publicationDate":"2016-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73868854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dusit Boonmekam, Suluck Namchote, M. Glaubrecht, D. Krailas
Traditionally, people in the Nan Province of Thailand eat raw fish, exposing them to a high risk of getting infected by fish-borne trematodes. The monitoring of helminthiasis among those people showed a high rate of infections by the intestinal fluke Haplorchis taichui , suggesting that also an epidemiologic study (of the epidemiology) of the intermediate hosts of this flat worm would be useful. In this study freshwater gastropods of thiarids and cyprinid fish (possible intermediate hosts) were collected around Bo Kluea and Pua District from April 2012 to January 2013. Both snails and fish were identified by morphology and their infections were examined by cercarial shedding and compressing. Cercariae and metacercariae of H. taichui were identified by morphology using 0.5 % neutral red staining. In addition a polymerase chain reaction of the internal transcribed spacer gene (ITS) was applied to the same samples. Among the three thiarid species present were Melanoides tuberculata , Mieniplotia (= Thiara or Plotia) scabra and Tarebia granifera only the latter species was infected with cercariae, with an infection rate or prevalence of infection of 6.61 % (115/1,740). In the 10 species of cyprinid fish (viz. Barbodes schwanenfeldi, Garra cambodgiensis, Hypsibarbus salweenensis, Mystacoleucus marginatus, Osparus pulchellus, Poropuntius deauratus, P. normani, Scaphiodonicthys acanthopterus, Systomus orphoides and S. stolitzkaenus ), metacercariae were found in all of them. Interestingly, B. schwanenfeldi, M. marginatus, P. deauratus, P. normani and S. orphoides had very high infection rates. Both morphometric and molecular identification confirmed those cercariae and metacercariae to represent the heterophyid Haplorchis taichui . Therefore, this study showed the high prevalence of this parasite both in its intermediate hosts as well as in humans. In order to prevent human infection, H. taichui has to be controlled.
{"title":"The Prevalence of Human Intestinal Fluke Infections, Haplorchis taichui, in Thiarid Snails and Cyprinid Fish in Bo Kluea District and Pua District, Nan Province, Thailand","authors":"Dusit Boonmekam, Suluck Namchote, M. Glaubrecht, D. Krailas","doi":"10.14456/SUSTJ.2016.10","DOIUrl":"https://doi.org/10.14456/SUSTJ.2016.10","url":null,"abstract":"Traditionally, people in the Nan Province of Thailand eat raw fish, exposing them to a high risk of getting infected by fish-borne trematodes. The monitoring of helminthiasis among those people showed a high rate of infections by the intestinal fluke Haplorchis taichui , suggesting that also an epidemiologic study (of the epidemiology) of the intermediate hosts of this flat worm would be useful. In this study freshwater gastropods of thiarids and cyprinid fish (possible intermediate hosts) were collected around Bo Kluea and Pua District from April 2012 to January 2013. Both snails and fish were identified by morphology and their infections were examined by cercarial shedding and compressing. Cercariae and metacercariae of H. taichui were identified by morphology using 0.5 % neutral red staining. In addition a polymerase chain reaction of the internal transcribed spacer gene (ITS) was applied to the same samples. Among the three thiarid species present were Melanoides tuberculata , Mieniplotia (= Thiara or Plotia) scabra and Tarebia granifera only the latter species was infected with cercariae, with an infection rate or prevalence of infection of 6.61 % (115/1,740). In the 10 species of cyprinid fish (viz. Barbodes schwanenfeldi, Garra cambodgiensis, Hypsibarbus salweenensis, Mystacoleucus marginatus, Osparus pulchellus, Poropuntius deauratus, P. normani, Scaphiodonicthys acanthopterus, Systomus orphoides and S. stolitzkaenus ), metacercariae were found in all of them. Interestingly, B. schwanenfeldi, M. marginatus, P. deauratus, P. normani and S. orphoides had very high infection rates. Both morphometric and molecular identification confirmed those cercariae and metacercariae to represent the heterophyid Haplorchis taichui . Therefore, this study showed the high prevalence of this parasite both in its intermediate hosts as well as in humans. In order to prevent human infection, H. taichui has to be controlled.","PeriodicalId":22107,"journal":{"name":"Silpakorn University Science and Technology Journal","volume":"7 1","pages":"29-37"},"PeriodicalIF":0.0,"publicationDate":"2016-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76793830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Probiotics applications in animal husbandry can increase feed intake, growth and immune responses. The aim of this study was to isolate the probiotic bacteria from piglet nostrils and test their efficacy in fattening pigs. Nasal swabs collected from 110 healthy piglets were processed on Man, Rogosa and Sharpe (MRS) agar plates for lactic acid bacteria isolation. The biochemical standard tests were used for identification of isolates organisms including: Gram staining, catalase production, tolerance to bile salts, acid and base and the utilization of proteins, starch and fat. The antimicrobial activity and resistance against antibiotics were also determined. Comparative 16S rRNA analysis confirmed the identity of selected strains as Enterococcus italicus . Thirty weaned piglets (mixed sex and 35-day old) were divided into three groups (five replicates with two animals) consisting of a control group, a positive control group fed with commercial feed and antibiotic, and an experimental group fed with commercial feed and probiotics. Porcine reproductive and respiratory syndrome (PRRS) vaccination were performed twice. Administration of Enterococcus italicus had no impact on average daily gain, whereas it affected feed intake and feed conversion ratio (p 0.05).
{"title":"Efficacy of Isolated Probiotic Bacteria from Piglet Nostrils in Fattening Pigs","authors":"C. Kasornpikul, Surawat Chalorsuntisakul","doi":"10.14456/SUSTJ.2016.8","DOIUrl":"https://doi.org/10.14456/SUSTJ.2016.8","url":null,"abstract":"Probiotics applications in animal husbandry can increase feed intake, growth and immune responses. The aim of this study was to isolate the probiotic bacteria from piglet nostrils and test their efficacy in fattening pigs. Nasal swabs collected from 110 healthy piglets were processed on Man, Rogosa and Sharpe (MRS) agar plates for lactic acid bacteria isolation. The biochemical standard tests were used for identification of isolates organisms including: Gram staining, catalase production, tolerance to bile salts, acid and base and the utilization of proteins, starch and fat. The antimicrobial activity and resistance against antibiotics were also determined. Comparative 16S rRNA analysis confirmed the identity of selected strains as Enterococcus italicus . Thirty weaned piglets (mixed sex and 35-day old) were divided into three groups (five replicates with two animals) consisting of a control group, a positive control group fed with commercial feed and antibiotic, and an experimental group fed with commercial feed and probiotics. Porcine reproductive and respiratory syndrome (PRRS) vaccination were performed twice. Administration of Enterococcus italicus had no impact on average daily gain, whereas it affected feed intake and feed conversion ratio (p 0.05).","PeriodicalId":22107,"journal":{"name":"Silpakorn University Science and Technology Journal","volume":"48 1","pages":"15-19"},"PeriodicalIF":0.0,"publicationDate":"2016-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81102363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Pongsapukdee, Pairoj Khawsittiwong, Maysiya Yamjaroenkit
Two symmetrical distributed test statistics, called Zm and Z0_New are proposed and their goodness-of-fit tests are compared with other available five test statistics: Wald-t, Score test, Z μ, ZY, and Z0, for overdispersion in Poisson regression model versus generalized Poisson model. Five thousand data sets in each condition of overdispersion parameters and sample sizes are simulated to perform the assessment of the models’ fits using those statistics, concerning the coverage probability and power of tests. Results show that the Zm test performs closely as good a Zμ and ZYtests but it tend to be better than the others when the sample size is large. Even if the Z0_New test has the largest power; however, in consideration for coverage probability and power of tests, the Zm test probably be more reliable. The Zm test statistic is interesting not only in its simplest form, with the reasonable coverage probability and power but also in its robust property of using median that needs fewer assumptions for its parent distribution.
{"title":"Test Statistics for Dispersion Parameter in Poisson Regression and Generalized Poisson Regression Models","authors":"V. Pongsapukdee, Pairoj Khawsittiwong, Maysiya Yamjaroenkit","doi":"10.14456/SUSTJ.2016.18","DOIUrl":"https://doi.org/10.14456/SUSTJ.2016.18","url":null,"abstract":"Two symmetrical distributed test statistics, called Zm and Z0_New are proposed and their goodness-of-fit tests are compared with other available five test statistics: Wald-t, Score test, Z μ, ZY, and Z0, for overdispersion in Poisson regression model versus generalized Poisson model. Five thousand data sets in each condition of overdispersion parameters and sample sizes are simulated to perform the assessment of the models’ fits using those statistics, concerning the coverage probability and power of tests. Results show that the Zm test performs closely as good a Zμ and ZYtests but it tend to be better than the others when the sample size is large. Even if the Z0_New test has the largest power; however, in consideration for coverage probability and power of tests, the Zm test probably be more reliable. The Zm test statistic is interesting not only in its simplest form, with the reasonable coverage probability and power but also in its robust property of using median that needs fewer assumptions for its parent distribution.","PeriodicalId":22107,"journal":{"name":"Silpakorn University Science and Technology Journal","volume":"7 1","pages":"31-36"},"PeriodicalIF":0.0,"publicationDate":"2016-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89809687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Water has so far been regarded as the best cutting fluid but its corrosion-influence on the work piece has necessitated searching for an alternative. Meanwhile, water-extract from fermented ground maize (WEFGM) processed from two (western yellow and kewesoke white) maize varieties have been considered having been previously found to contain corrosion inhibitors. Its density, viscosity and specific heat capacity (SHC) were determined and compared with that of water. The results showed that white and yellow WEFGM give densities with percentage decrease of 0.1 and 1.0% relative to water respectively. Using Stokes’s law, the ratios of the viscosities for white WEFGM, yellow WEFGM and water were 1.22: 1.17: 1.00 and 1.20: 1.26: 1.00 respectively for the smallest (diameter of 0.45 cm) and biggest (diameter of 1.4 cm) spheres. T-test shows that there is no significant difference in the densities and viscosities values of all the samples (P<0.05). The result of SHC gives the ratio of 1.16:1.03:1.00 respectively for white, yellow and water samples with white and yellow having higher SHC than water with a difference of 15.7 and 3.6% respectively. Higher thermal capacity, presence of corrosion inhibitors with density and viscosity not quite dissimilar, viewed holistically, makes WEFGM having a better prospect than water as cutting fluid.
{"title":"Determination of Thermo Physical Properties of Water-Extract from Fermented Ground Maize (WEFGM) as Possible Alternative to Water Use as Cutting Fluid","authors":"T. A. Yusuf","doi":"10.14456/SUSTJ.2016.20","DOIUrl":"https://doi.org/10.14456/SUSTJ.2016.20","url":null,"abstract":"Water has so far been regarded as the best cutting fluid but its corrosion-influence on the work piece has necessitated searching for an alternative. Meanwhile, water-extract from fermented ground maize (WEFGM) processed from two (western yellow and kewesoke white) maize varieties have been considered having been previously found to contain corrosion inhibitors. Its density, viscosity and specific heat capacity (SHC) were determined and compared with that of water. The results showed that white and yellow WEFGM give densities with percentage decrease of 0.1 and 1.0% relative to water respectively. Using Stokes’s law, the ratios of the viscosities for white WEFGM, yellow WEFGM and water were 1.22: 1.17: 1.00 and 1.20: 1.26: 1.00 respectively for the smallest (diameter of 0.45 cm) and biggest (diameter of 1.4 cm) spheres. T-test shows that there is no significant difference in the densities and viscosities values of all the samples (P<0.05). The result of SHC gives the ratio of 1.16:1.03:1.00 respectively for white, yellow and water samples with white and yellow having higher SHC than water with a difference of 15.7 and 3.6% respectively. Higher thermal capacity, presence of corrosion inhibitors with density and viscosity not quite dissimilar, viewed holistically, makes WEFGM having a better prospect than water as cutting fluid.","PeriodicalId":22107,"journal":{"name":"Silpakorn University Science and Technology Journal","volume":"66 1","pages":"51-60"},"PeriodicalIF":0.0,"publicationDate":"2016-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83664442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Internal classroom conditions are important aspects of the learning environment of students. A suite of relevant guidelines for acoustic, visual and thermal comfort parameters in naturally and/or mechanically ventilated classrooms in tropical environments is proposed. Data measured during winter and rainy seasons in a typical provincial school in central Thailand were then compared. Only thermal parameters, viz. wet bulb globe temperature, air temperature and relative humidity met the proposed guidelines values at all times during the school year. Mean sound levels and signal-to-noise ratios did not meet the requirements at some time during this period. Mean illuminance in classrooms only reached proposed guideline values when all internal lights were operating which occurred mainly during the rainy season. Illuminance in front of blackboards and whiteboards never reached proposed thresholds at any time.
{"title":"Annual Variations of Environmental Conditions in Naturally Ventilated Classrooms in a Tropical Environment: Comparison with Proposed Guidelines","authors":"G. Sripongpun, K. Srisawat, D. Hawker","doi":"10.14456/SUSTJ.2016.19","DOIUrl":"https://doi.org/10.14456/SUSTJ.2016.19","url":null,"abstract":"Internal classroom conditions are important aspects of the learning environment of students. A suite of relevant guidelines for acoustic, visual and thermal comfort parameters in naturally and/or mechanically ventilated classrooms in tropical environments is proposed. Data measured during winter and rainy seasons in a typical provincial school in central Thailand were then compared. Only thermal parameters, viz. wet bulb globe temperature, air temperature and relative humidity met the proposed guidelines values at all times during the school year. Mean sound levels and signal-to-noise ratios did not meet the requirements at some time during this period. Mean illuminance in classrooms only reached proposed guideline values when all internal lights were operating which occurred mainly during the rainy season. Illuminance in front of blackboards and whiteboards never reached proposed thresholds at any time.","PeriodicalId":22107,"journal":{"name":"Silpakorn University Science and Technology Journal","volume":"14 1","pages":"37-50"},"PeriodicalIF":0.0,"publicationDate":"2016-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85695304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Wongkasemjit, Panupong Chuntanalerg, S. Kulthipanja, T. Chaisuwan
Bioethanol fuel produced directly from the renewable biomass is one of the truly important energy developments in Thailand. Typically, the ethanol concentration after fermentation is quite low. Normally, ethanol, as called anhydrous ethanol or absolute ethanol used for fuel energy, is required 99.5% purity or concentration. It is necessary to use energy-effective separation method for concentrating ethanol up to the fuel level. There are several techniques used to purify ethanol such as distillation, membrane technology and molecular sieve separation. Recently, the less energyintensive technique of pervaporation is found to be a promising method for producing high-purity ethanol, but the overall economic feasibility is dependent upon the membrane. Thus, in this review, various membranes reported in literature are discussed and compared as to their advantages, disadvantages, and separation performance via the pervaporation process. It is found that different membranes provide different performances and advantages in ethanol separation. Other parameters governing the separation performance, such as temperature, ethanol concentration, membrane production method, and production cost, are also included.
{"title":"Review on “Membranes for Ethanol-Water Separation”","authors":"S. Wongkasemjit, Panupong Chuntanalerg, S. Kulthipanja, T. Chaisuwan","doi":"10.14456/SUSTJ.2016.21","DOIUrl":"https://doi.org/10.14456/SUSTJ.2016.21","url":null,"abstract":"Bioethanol fuel produced directly from the renewable biomass is one of the truly important energy developments in Thailand. Typically, the ethanol concentration after fermentation is quite low. Normally, ethanol, as called anhydrous ethanol or absolute ethanol used for fuel energy, is required 99.5% purity or concentration. It is necessary to use energy-effective separation method for concentrating ethanol up to the fuel level. There are several techniques used to purify ethanol such as distillation, membrane technology and molecular sieve separation. Recently, the less energyintensive technique of pervaporation is found to be a promising method for producing high-purity ethanol, but the overall economic feasibility is dependent upon the membrane. Thus, in this review, various membranes reported in literature are discussed and compared as to their advantages, disadvantages, and separation performance via the pervaporation process. It is found that different membranes provide different performances and advantages in ethanol separation. Other parameters governing the separation performance, such as temperature, ethanol concentration, membrane production method, and production cost, are also included.","PeriodicalId":22107,"journal":{"name":"Silpakorn University Science and Technology Journal","volume":"1 1","pages":"61-73"},"PeriodicalIF":0.0,"publicationDate":"2016-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79785473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sarawalee Thanasilp, J. Schwank, V. Meeyoo, S. Pengpanich, M. Hunsom
The liquid phase catalytic oxydehydration of glycerol to acrylic acid over supported silicotungstic acid (SiW) catalysts was carried out in the batch reactor. The effect of oxidizing agent concentration (H2O2), reaction temperatures (70 and 90 °C), types of supports (HZSM-5, SiO2 and Al2O3) and SiW loading (20-60 wt.% based on support) on the conversion and product yield were investigated. The addition of H2O2 and supported SiW catalysts significantly conducted the synergetic positive effects on glycerol conversion and acrylic acid yields as well as other desired products including glycolic acid, formic acid, acetic acid, acrolein and acrylic acid. High reaction temperature was able to enhance high glycerol conversion as the same yield of all desired products. The BET surface area of supported SiW catalysts played much more important role in the activities of oxydehydration of glycerol that this oxydehydration was more remarkable than the acidity of catalysts. Among all supported SiW catalysts, the SiW/HZSM-5 with SiW loading of 30 wt.% exhibited the highest glycerol conversion (85.54%) with the production acrylic acid yield of 30.57 % over 2.74 M H2O2 at 90 °C. The kinetics of glycerol conversion to desired products over supported SiW catalyst was explored.
在间歇式反应器中，采用负载型硅钨酸(SiW)催化剂催化甘油液相氧化脱水制丙烯酸。考察了氧化剂浓度(H2O2)、反应温度(70℃和90℃)、载体类型(HZSM-5、SiO2和Al2O3)和SiW负载(根据载体不同，负载比例为20 ~ 60 wt.%)对转化率和产率的影响。H2O2和负载SiW催化剂的加入对甘油转化率和丙烯酸收率以及其他期望产物(乙醇酸、甲酸、乙酸、丙烯醛和丙烯酸)产生了显著的协同正效应。高的反应温度能够提高高的甘油转化率，使所有期望产物的产率相同。负载型SiW催化剂的BET表面积对甘油氧化脱水活性的影响比催化剂的酸度更显著。在负载SiW催化剂中，SiW/HZSM-5在负载SiW为30 wt.%时，甘油转化率最高(85.54%)，在2.74 M H2O2条件下，在90°C条件下的丙烯酸收率为30.57%。研究了负载型SiW催化剂上甘油转化为所需产物的动力学。
{"title":"Liquid Phase Oxydehydration of Glycerol to Acrylic Acid over Supported Silicotungstic Acid Catalyst: Influence of Reaction Parameters","authors":"Sarawalee Thanasilp, J. Schwank, V. Meeyoo, S. Pengpanich, M. Hunsom","doi":"10.14456/SUSTJ.2016.16","DOIUrl":"https://doi.org/10.14456/SUSTJ.2016.16","url":null,"abstract":"The liquid phase catalytic oxydehydration of glycerol to acrylic acid over supported silicotungstic acid (SiW) catalysts was carried out in the batch reactor. The effect of oxidizing agent concentration (H2O2), reaction temperatures (70 and 90 °C), types of supports (HZSM-5, SiO2 and Al2O3) and SiW loading (20-60 wt.% based on support) on the conversion and product yield were investigated. The addition of H2O2 and supported SiW catalysts significantly conducted the synergetic positive effects on glycerol conversion and acrylic acid yields as well as other desired products including glycolic acid, formic acid, acetic acid, acrolein and acrylic acid. High reaction temperature was able to enhance high glycerol conversion as the same yield of all desired products. The BET surface area of supported SiW catalysts played much more important role in the activities of oxydehydration of glycerol that this oxydehydration was more remarkable than the acidity of catalysts. Among all supported SiW catalysts, the SiW/HZSM-5 with SiW loading of 30 wt.% exhibited the highest glycerol conversion (85.54%) with the production acrylic acid yield of 30.57 % over 2.74 M H2O2 at 90 °C. The kinetics of glycerol conversion to desired products over supported SiW catalyst was explored.","PeriodicalId":22107,"journal":{"name":"Silpakorn University Science and Technology Journal","volume":"8 1","pages":"9-21"},"PeriodicalIF":0.0,"publicationDate":"2016-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81979289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A gene encoding for cellobiohydrolase (PEcbh) from P. eryngii was cloned by using RT-PCR 3’ and 5’ RACE techniques. The result showed that the PEcbh was 1377 bp nucleotide sequence encoded for 459-deduced amino acid. Analysis of predicted protein revealed that PEcbh consisted of a glycosyl hydrolase family 7 domain but lacked of cellulose binding domain, a calculated molecular weight of 49.3 kDa and a pI of 5.3. The PEcbh was cloned into pET28a (+) to obtain a recombinant pET/PEcbh and expressed in E. coli BL21 (DE3). The optimal conditions of PEcbh expression were 0.2 mM IPTG, 1 h induction time at 180C and 4 h post-induction time. The CMCase activity could be detected, but at a low activity. This is probably due to a lack of the cellulose binding domain in PEcbh. Expression of PEcbh in E. coli BL21 (DE3) and Rosetta (DE3) were compared and the results indicated that CMCase activity in Rosetta (DE3) was higher than in BL21 about 2 times.
{"title":"Cloning and Expression of the Cellulase Gene from the King Oyster Mushroom, Pleurotus eryngii.","authors":"U. Romruen, E. Bangyeekhun","doi":"10.14456/SUSTJ.2016.17","DOIUrl":"https://doi.org/10.14456/SUSTJ.2016.17","url":null,"abstract":"A gene encoding for cellobiohydrolase (PEcbh) from P. eryngii was cloned by using RT-PCR 3’ and 5’ RACE techniques. The result showed that the PEcbh was 1377 bp nucleotide sequence encoded for 459-deduced amino acid. Analysis of predicted protein revealed that PEcbh consisted of a glycosyl hydrolase family 7 domain but lacked of cellulose binding domain, a calculated molecular weight of 49.3 kDa and a pI of 5.3. The PEcbh was cloned into pET28a (+) to obtain a recombinant pET/PEcbh and expressed in E. coli BL21 (DE3). The optimal conditions of PEcbh expression were 0.2 mM IPTG, 1 h induction time at 180C and 4 h post-induction time. The CMCase activity could be detected, but at a low activity. This is probably due to a lack of the cellulose binding domain in PEcbh. Expression of PEcbh in E. coli BL21 (DE3) and Rosetta (DE3) were compared and the results indicated that CMCase activity in Rosetta (DE3) was higher than in BL21 about 2 times.","PeriodicalId":22107,"journal":{"name":"Silpakorn University Science and Technology Journal","volume":"70 1","pages":"22-30"},"PeriodicalIF":0.0,"publicationDate":"2016-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87359568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Antimicrobial-resistant bacteria are an important medical concern. This problem might be resulted from an inappropriate use of antibiotics. The review of antibiotic drug use could help understanding the cause of drug resistance and eventually resolve the problem. Our retrospective cohort study aimed to review meropenem use at Hua-hin hospital, Prachuap Khiri Khan, including therapeutic indications, dosage regimens and duration of sepsis treatment. Data were gathered from patients admitted in the hospital during January to December 2013. The sources of data included patient medical records, patient database from computer program and in-house form of meropenem evaluation. A total of thirty-six cases were evaluated, 17 cases (47.2%) were male, and the average age was 58.3 (±19.9) years. There were 22 cases (61.1%) and 14 cases (38.9%) receiving meropenem as empirical and definitive therapy, respectively. According to meropenem use evaluation, there were 29 (80.6%), 33 (91.7%) and 24 (85.7%) cases that met the criteria for indication, dosage regimen, and treatment duration, respectively. Only 17 cases (60.7%; n=28) met all three criteria for appropriate meropenem use. Thus, with our finding, the role of healthcare professionals in reducing the inappropriate use of antibiotics for the entire course of treatment should be encouraged.
{"title":"Evaluation of Meropenem Use Among Septic Patients at Hua Hin Hospital, Thailand","authors":"Wandee Sumret, Kamonphop Saowakul, Thirawan Haephruek, Penprapa Pengsombat, Witchuda Kwanmuang, W. Santimaleeworagun","doi":"10.14456/SUSTJ.2016.2","DOIUrl":"https://doi.org/10.14456/SUSTJ.2016.2","url":null,"abstract":"Antimicrobial-resistant bacteria are an important medical concern. This problem might be resulted from an inappropriate use of antibiotics. The review of antibiotic drug use could help understanding the cause of drug resistance and eventually resolve the problem. Our retrospective cohort study aimed to review meropenem use at Hua-hin hospital, Prachuap Khiri Khan, including therapeutic indications, dosage regimens and duration of sepsis treatment. Data were gathered from patients admitted in the hospital during January to December 2013. The sources of data included patient medical records, patient database from computer program and in-house form of meropenem evaluation. A total of thirty-six cases were evaluated, 17 cases (47.2%) were male, and the average age was 58.3 (±19.9) years. There were 22 cases (61.1%) and 14 cases (38.9%) receiving meropenem as empirical and definitive therapy, respectively. According to meropenem use evaluation, there were 29 (80.6%), 33 (91.7%) and 24 (85.7%) cases that met the criteria for indication, dosage regimen, and treatment duration, respectively. Only 17 cases (60.7%; n=28) met all three criteria for appropriate meropenem use. Thus, with our finding, the role of healthcare professionals in reducing the inappropriate use of antibiotics for the entire course of treatment should be encouraged.","PeriodicalId":22107,"journal":{"name":"Silpakorn University Science and Technology Journal","volume":"24 1","pages":"21-26"},"PeriodicalIF":0.0,"publicationDate":"2016-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81185736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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