Systematic and applied microbiology最新文献_第5页

Cellulosispirillum alkaliphilum, gen. nov., sp. nov., an obligately anaerobic cellulotrophic member of the phylum Fibrobacterota from soda lakes 嗜碱纤维素菌属（Cellulosispirillum alkaliphilum, gen. nov., sp. nov.），是碱湖纤维杆菌门的一种专性厌氧纤维素营养菌

IF 3.3 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Systematic and applied microbiology

Pub Date : 2025-06-02 DOI: 10.1016/j.syapm.2025.126623

Dimitry Y. Sorokin , Alexander Y. Merkel , Tatjana V. Khizhniak

Intensive microbiology studies of the past several decades of soda lakes, uncovered a rich functional diversity of haloalkaliphilic microbes driving carbon, nitrogen and sulfur cycles in these unique double-extreme habitats. One of the unexpected finding was a discovery there of aerobic extremely halophilic cellulotrophic natronoarchaea. Yet, little is still known about the identity of the soda lake bacteria able to use native cellulose as growth substrate, except for a single case of an anaerobic clostridium. In this work we present results of phenotypic and functional genomic analysis of an anaerobic bacterium, strain ANBcel5^T, enriched from hypersaline Siberian soda lakes with amorphous cellulose as growth substrate. Phylogenetic analysis placed the isolate into the family Chitinispirillaceae in the phylum Fibrobacterota as a new genus and species lineage with the 16S rRNA gene identity and Relative Evolutionary Divergence (RED) to its only known species Chitinispirillum alkaliphilum ACht6–1^T of 95.2 % and 0.847, respectively. In contrast, despite obvious morphological resemblance to ACht6–1^T, strain ANBcel5^T is a narrow cellulose-utilizing fermentative anaerobe fermenting cellulose and cellobiose to acetate, H₂ and succinate. It is a moderately salt-tolerant obligate alkaliphile growing optimally at 0.6 M total Na⁺ as carbonates and pH 9.5. Functional genome analysis of the isolate revealed the presence of multiple genes encoding extracellular endocellulases from the GH families 5 and 9, three sodium-translocating membrane complexes and osmolytes Nε-acetyl-β-lysine and glycine betaine biosynthesis. The bacterium is proposed to be classified as Cellulosispirillum alkaliphilum gen. nov., sp. nov. (DSM 113825 = UQM 41584).

对过去几十年的苏打湖进行了深入的微生物学研究，发现了在这些独特的双极端栖息地中驱动碳、氮和硫循环的亲卤碱性微生物的丰富功能多样性。其中一个意想不到的发现是在那里发现了有氧极端嗜盐的纤维素营养钠古菌。然而，除了一种厌氧梭状芽胞杆菌外，人们对能够使用天然纤维素作为生长基质的钠湖细菌的身份知之甚少。在这项工作中，我们介绍了厌氧细菌菌株ANBcel5T的表型和功能基因组分析结果，菌株ANBcel5T富集于高盐西伯利亚苏打湖中，无定形纤维素作为生长基质。系统发育分析结果表明，该分离物属于纤维细菌门Chitinispirillaceae新属和新种，其16S rRNA基因同源性为95.2%，与唯一已知种Chitinispirillum alkaliphilum ACht6-1T的相对进化差异（RED）分别为0.847%。相比之下，尽管形态与ACht6-1T明显相似，菌株ANBcel5T是一种狭窄的利用纤维素的发酵厌氧菌，将纤维素和纤维素二糖发酵成乙酸、H2和琥珀酸盐。它是一种中等耐盐的专性亲碱植物，在0.6 M总Na+和9.5 pH下生长最佳。功能基因组分析显示，该菌株存在多个编码生长激素家族5和9的细胞外内胞酶、3个钠转运膜复合物和渗透酶nε -乙酰-β-赖氨酸和甘氨酸甜菜碱生物合成的基因。该细菌被建议归类为Cellulosispirillum alkaliphilum gen. nov., sp. nov. （DSM 113825 = UQM 41584）。

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引用次数: 0

Halosimplex amylolyticum sp. nov., Halosimplex halobium sp. nov., Halosimplex marinum sp. nov., Halosimplex rarum sp. nov., Halovenus amylolytica sp. nov., Halovenus halobia sp. nov., and Halovenus marina sp. nov., halophilic archaea isolated from a marine tidal flat, a marine solar saltern, three coarse sea salts, and two saline lakes 从海洋潮滩、海洋太阳盐沼、三种粗海盐和两个盐湖中分离出的嗜盐古菌，分别是：嗜盐单孢菌、嗜盐单孢菌、海洋单孢菌、稀有嗜盐单孢菌、嗜盐单孢菌、嗜盐单孢菌、嗜盐单孢菌和嗜盐单孢菌

IF 3.3 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Systematic and applied microbiology

Pub Date : 2025-06-01 DOI: 10.1016/j.syapm.2025.126626

Ya-Ling Mao, Shun Tan, Bei-Bei Wang, Xiao-Yan Yang, Jing Hou, Heng-Lin Cui

Seven novel halophilic archaeal strains DYHT-AS-1^T, GDY60^T, TS25^T, XH63^T, SHR40^T, SYNS179^T, and ZY30^T were isolated from tidal flat, saline lakes, marine solar saltern and coarse sea salts from different regions of China. Metagenomic and amplicon analyses indicated that the abundance of these seven strains in respective habitats was low. Phylogenetic and comparative genomic analyses indicated that strains DYHT-AS-1^T, GDY60^T, TS25^T, and XH63^T formed a tight cluster with Halosimplex species, exhibiting high 16S rRNA gene sequence similarity (90.4–99.4 %). The ANI, dDDH, and AAI values among these four strains and current Halosimplex species were 80.8–92.3 %, 24.5–53.3 %, and 77.8–91.5 %, respectively. Strains SHR40^T, SYNS179^T, and ZY30^T were related to Halovenus species, with 16S rRNA gene sequence similarities ranging from 88.8 % to 98.4 %. The ANI, dDDH, and AAI values among these three strains and current Halovenus species were 69.9–77.8 %, 19.4–21.5 %, and 62.1–78.1 %, respectively. These values are significantly lower than the thresholds for species demarcation. The optimal growth conditions for these seven strains in terms of NaCl, MgCl₂, temperature, and pH were 3.1–3.4 M, 0.05–0.5 M, 35–40 °C, and 6.5–7.5, respectively. According to phenotypic differences in nutrition, biochemical activity, and antibiotic sensitivity, these seven strains can be distinguished from their related species. Based on the above results, strains DYHT-AS-1^T, GDY60^T, TS25^T, and XH63^T represent four new species of the genus Halosimplex, and strains SHR40^T, SYNS179^T, and ZY30^T represent three novel species of the genus Halovenus.

从中国不同地区的潮滩、盐湖、海洋太阳盐沼和粗海盐中分离到7株新的嗜盐古细菌DYHT-AS-1T、GDY60T、TS25T、XH63T、SHR40T、SYNS179T和ZY30T。宏基因组和扩增子分析表明，这7种菌株在各自生境的丰度较低。系统发育和比较基因组分析表明，菌株DYHT-AS-1T、GDY60T、TS25T和XH63T与Halosimplex属形成紧密的集群，16S rRNA基因序列相似性较高（90.4 ~ 99.4%）。4株菌株与现种的ANI、dDDH和AAI值分别为80.8% ~ 92.3%、24.5 ~ 53.3%和77.8 ~ 91.5%。菌株SHR40T、SYNS179T和ZY30T与Halovenus属有亲缘关系，其16S rRNA基因序列相似性在88.8% ~ 98.4%之间。3种菌株与现种的ANI、dDDH和AAI值分别为69.9 ~ 77.8%、19.4 ~ 21.5%和62.1 ~ 78.1%。这些值明显低于物种划分的阈值。7株菌株的最佳生长条件（NaCl、MgCl 2、温度、pH）分别为3.1 ~ 3.4 M、0.05 ~ 0.5 M、35 ~ 40℃和6.5 ~ 7.5。根据营养、生化活性和抗生素敏感性的表型差异，可以将这7株菌株与其亲缘种区分开来。基于以上结果，菌株DYHT-AS-1T、GDY60T、TS25T和XH63T代表Halosimplex属的4个新种，菌株SHR40T、SYNS179T和ZY30T代表Halosimplex属的3个新种。

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引用次数: 0

Classification of MAGs associated with trace gas metabolism in volcanic soils named following SeqCode rules 火山土壤中与微量气体代谢相关的mag分类，命名遵循SeqCode规则

IF 3.3 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Systematic and applied microbiology

Pub Date : 2025-05-28 DOI: 10.1016/j.syapm.2025.126622

Shamik Roy , Gary M. King , Marcela Hernández

Trace gas metabolism is important for nutrient flow in all ecosystems, particularly volcanic ecosystems. Microbes in volcanic ecosystems are among the early colonisers and can play key roles in ecological succession. Here, we describe the taxonomic and functional characteristics of two new metagenome-assembled genomes (MAGs), one belonging to Bacteria (MAG_1957-2.1) and one to Archaea (MAG_C2-3), retrieved from soils in volcanoes located in Chile (Llaima) and the USA (Kilauea), respectively. MAG_1957-2.1 has a genome size of 6.36 Mb with 96.21 % completeness. MAG_C2-3 has a genome size of 3.02 Mb with 97.57 % completeness. Phylogenetic analyses of the bacterial MAGs placed MAG_1957-2.1 in the class Ktedonobacteria, while the archaeal MAG_C2-3 was placed in the class Nitrososphaeria. Functional characterisation for potential trace gas metabolism showed that MAG_1957-2.1 contains a coxL gene encoding the large subunit of form I carbon monoxide dehydrogenase (CoxL), which is associated with the oxidation of carbon monoxide (CO). It also contains the form I cox gene cluster with a coxMSL arrangement. On the other hand, MAG_C2-3 contains gene subunit A (amoA) as well as subunit B (amoB), which encode for ammonia monooxygenase, the enzyme that catalyses the oxidation of ammonia. Based on the sequence characteristics and phylogenomic analyses we propose the names Paraktedonobacter carboxidivorans sp. nov for MAG_1957-2.1 and Nitrososphaera maunauluensis sp. nov for MAG_C2-3. The names are proposed following the rules of the published Code of Nomenclature of Prokaryotes Described from Sequence Data (SeqCode).

微量气体代谢是所有生态系统，特别是火山生态系统养分流动的重要组成部分。火山生态系统中的微生物是早期的殖民者之一，可以在生态演替中发挥关键作用。本文描述了两个新的宏基因组组装基因组（MAGs）的分类和功能特征，一个属于细菌（MAG_1957-2.1），一个属于古细菌（MAG_C2-3），分别来自智利（Llaima）和美国（Kilauea）火山的土壤。MAG_1957-2.1的基因组大小为6.36 Mb，完整性为96.21%。MAG_C2-3的基因组大小为3.02 Mb，完整性为97.57%。细菌MAG_1957-2.1归属于Ktedonobacteria纲，而古细菌MAG_C2-3归属于亚硝基球藻纲。潜在微量气体代谢的功能表征表明，MAG_1957-2.1含有一个coxL基因，该基因编码I型一氧化碳脱氢酶（coxL）的大亚基，该亚基与一氧化碳（CO）的氧化有关。它还包含形式1 cox基因簇与coxMSL的安排。另一方面，MAG_C2-3含有基因亚基A （amoA）和亚基B (amoB)，它们编码氨单加氧酶，氨单加氧酶是催化氨氧化的酶。根据序列特征和系统基因组分析，我们提出将MAG_1957-2.1命名为Paraktedonobacter carboxidivorans sp. nov，将MAG_C2-3命名为亚硝基sphaera maunauluensis sp. nov。这些名称是根据已出版的《序列数据描述原核生物命名规范》（SeqCode）的规则提出的。

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引用次数: 0

Magnetovirga frankeli gen. Nov., sp. nov., a magnetotactic bacterium isolated from the Salton Sea, California, that represents a novel lineage in the Gammaprotoeobacteria frankeli Magnetovirga gen. Nov., sp. Nov.，一种从加利福尼亚索尔顿海分离出来的趋磁细菌，它代表了伽玛原细菌中的一个新谱系

IF 3.3 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Systematic and applied microbiology

Pub Date : 2025-05-24 DOI: 10.1016/j.syapm.2025.126621

Mila Sirinelli-Kojadinovic , Elsa C.A. Turrini , Emma Ropion , Béatrice Alonso , Marine Bergot , Emilie Gachon , Philippe Ortet , Paul E.D. Soto-Rodriguez , Caroline L. Monteil , Christopher T. Lefevre

A magnetotactic bacterium, designated strain SS-5^T, was isolated from the Salton Sea, a highly saline lake in California, USA, and cultivated in axenic culture. The Gram-negative cells of strain SS-5^T are relatively small and rod-shaped and possess a single polar flagellum (monotrichous). This strain is a magnetotactic bacterium producing magnetite nanocrystals aligned in one chain per cell. Strain SS-5^T is a microaerophile that grows chemolithoautotrophically while reducing oxygen as a terminal electron acceptor. Optimal growth occurred at pH 7.0–7.3 and 28–36 °C with thiosulfate used as an electron donor and sodium bicarbonate as a carbon source. Based on its genome sequence, the G + C content is 61.6 %. Phylogenomic and phylogenetic analyses indicate that strain SS-5^T belongs to the Sedimenticolaceae family within the Gammaproteobacteria class. Based on average amino acid identity, strain SS-5^T can be considered as a novel species of a new genus, for which the name Magnetovirga frankeli is proposed. The type strain of M. frankeli is SS-5^T (=DSM 116211^T = JCM 39467^T).

从美国加州高盐湖Salton Sea中分离出一株趋磁细菌SS-5T，并进行无菌培养。菌株SS-5T革兰氏阴性细胞相对较小，呈杆状，具有单极鞭毛（单色）。这种菌株是一种趋磁细菌，每个细胞产生排成一条链的磁铁矿纳米晶体。菌株SS-5T是一种微亲氧菌，它以化学岩石自养的方式生长，同时作为终端电子受体还原氧。以硫代硫酸盐为电子供体，碳酸氢钠为碳源，在pH 7.0-7.3和28-36℃条件下生长最佳。根据其基因组序列，G + C含量为61.6%。系统发育和系统发育分析表明，菌株SS-5T属于伽玛变形菌纲中的沉积菌科。基于平均氨基酸特征，菌株SS-5T可视为一个新属的新种，并建议将其命名为Magnetovirga frankeli。M. frankeli型菌株为SS-5T （=DSM 116211T = JCM 39467T）。

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引用次数: 0

Macrococcus capreoli sp. nov., a new fosfomycin resistant species isolated from feces and nasal swabs of deer 从鹿粪便和鼻拭子中分离到一种新的磷霉素耐药菌

IF 3.3 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Systematic and applied microbiology

Pub Date : 2025-05-23 DOI: 10.1016/j.syapm.2025.126620

Carolin J. Schiffer , Matthias A. Ehrmann

Five strains of Gram-stain-positive, non-motile, and coccoid-shaped bacteria, designated as TMW 2.2395^T, TMW 2.2628, TMW 2.2670, TMW 2.2756 and TMW 2.2757 were isolated from feces and nasal mucosa of wild living roe deer (Capreolus capreolus) and red deer (Cervus elaphus), respectively. The isolates share identical 16S rRNA gene sequences and are classified within the genus Macrococcus based on 16S and MALDI-TOF MS analyses. Phylogenetic analysis revealed that average nucleotide identity (ANI) values were below the accepted thresholds for prokaryotic species delineation, with the type strains of M. goetzii, M. bohemicus and M. epidermidis showing the highest relatedness values (79.59 %, 79.30 % and 79.52 %, respectively). Digital DNA–DNA hybridization (dDDH) values were below 23 % confirming that all five strains belong to a new species within the genus Macrococcus. All strains were catalase and oxidase positive, grew optimally at 37 °C and pH 7.0, but demonstrated considerably lower salt tolerance (< 7.5 % w/v nit iterativ) compared to other species in the genus. Moreover, the data of this study suggests that members of this new species possess an intrinsic resistance to fosfomycin, putatively mediated by a fosB homologue. The genomes range from 2.4 and 2.5 Mb in size, with a G+C content of 34.8 to 35.1 %. The primary respiratory quinone of the type strain TMW 2.2395^T is Menaquinone 6 (MK-6, 98.6 %), the cell wall peptidoglycan type is A3α L-Lys-Gly₃-L-Ser and the major fatty acids are C_14:0, C_16:0 and C_18:0. Phylogenetic, phenotypic and chemotaxonomic data collectively suggest that these strains represent a novel species of the genus Macrococcus, for which the name Macrococcus capreoli sp. nov. is proposed. The type strain is TMW 2.2395^T = DSM 113939^T = LMG 32618^T.

从野生狍子（Capreolus Capreolus）和马鹿（Cervus elaphus）的粪便和鼻黏膜中分别分离到革兰氏染色阳性、非运动和球状细菌TMW 2.2395T、TMW 2.2628、TMW 2.2670、TMW 2.2756和TMW 2.2757 5株。根据16S和MALDI-TOF质谱分析，分离株具有相同的16S rRNA基因序列，属于巨球菌属。系统发育分析结果显示，三种菌株的平均核苷酸同源性（ANI）均低于原核生物种划分的可接受阈值，其中goetzii型菌株、波西米亚型菌株和表皮型菌株的亲缘性最高，分别为79.59%、79.30%和79.52%。数字DNA-DNA杂交（dDDH）值低于23%，证实所有5株菌株属于大球菌属的新种。所有菌株过氧化氢酶和氧化酶均阳性，在37°C和pH 7.0条件下生长最佳，但耐盐性明显降低(<；7.5% w/v单位迭代率)与该属其他物种相比。此外，本研究的数据表明，这个新物种的成员对磷霉素具有内在的抗性，据推测是由磷sb同源物介导的。基因组大小在2.4 ~ 2.5 Mb之间，G+C含量为34.8% ~ 35.1%。菌株TMW 2.2395T主要呼吸醌为甲基萘醌6 (MK-6, 98.6%)，细胞壁肽聚糖类型为A3α L-Lys-Gly3-L-Ser，主要脂肪酸为C14:0、C16:0和C18:0。系统发育、表型和化学分类数据共同表明，这些菌株代表了巨球菌属的一个新种，因此建议将其命名为巨球菌。型应变为TMW 2.2395T = DSM 113939T = LMG 32618T。

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引用次数: 0

Unveiling the hidden microbiome: a microbiological exploration of untouched burial crypts in Krakow, Poland 揭开隐藏的微生物群：对波兰克拉科夫未被触及的埋葬地穴的微生物探索

IF 3.3 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Systematic and applied microbiology

Pub Date : 2025-05-21 DOI: 10.1016/j.syapm.2025.126618

Patrycja Tarnawska , Aleksandra Burkowska-But , Maria Swiontek Brzezińska , Anna Drążkowska , Adriana Osińska , Maciej Walczak

Cultural heritage objects provide valuable historical information, but can also harbour biological threats. Still, little is said about the potential risks that may await unaware researchers, conservators, and archaeologists. Our work discusses the study results from the crypts in Krakow, which were opened for the first time. The human and coffin remains were examined. The number of actinomycetes, other mesophilic bacteria, bacterial spores, and xerophilic and non-xerophilic fungi was determined. In general, a low number of microbes was observed. However, scanning electron microscope (SEM) images showed many bacterial conglomerates and confirmed that microbial activity affected the fibres covering cadavers in the crypts. The most abundant were mesophilic bacteria, followed by bacterial spores and actinomycetes. They reached up to 10⁷ CFU/g in fabric remains, 5.2 × 10⁶ CFU/g in burial remains, and 1.6 × 10⁶ CFU/g found under the coffin, and above 7.5 × 10⁵ CFU/g for xerophilic and non-xerophilic fungi. NGS (Next-Generation Sequencing) results suggested that the low presence of microorganisms may be due to the dominance of unculturable or long-growing bacteria belonging to Mycobacterium, such as M. coloregonium, M. arupense, and M. pinnipedii. Moreover, other obligatory/non-obligatory pathogens, Bacteroides fragilis, Clostridium botulinum, Coxiella burnetii, Clostridium tetani, Corynebacterium diphtheriae, Enterobacter cloacae, Escherichia coli, Legionella pneumophila, Mycobacterium leprae, Rhodococcus equi, and Staphylococcus aureus have been recorded in examined samples, with the dominance in bone samples. Results indicate the risk of dangerous pathogens present in historical objects, the impact on health may be severe, and the need to use personal protective equipment and proper measures to control the physical conditions of crypts.

文化遗产提供了宝贵的历史信息，但也可能隐藏着生物威胁。然而，对于可能等待不知情的研究人员、保护人员和考古学家的潜在风险，几乎没有人说。我们的工作讨论了克拉科夫地穴的研究结果，这些地穴是第一次打开的。人体和棺材的残骸被检查了。测定了放线菌、其他嗜中温细菌、细菌孢子、嗜干和非嗜干真菌的数量。总的来说，观察到的微生物数量很少。然而，扫描电子显微镜（SEM）图像显示了许多细菌集合体，并证实微生物活动影响了隐窝中覆盖尸体的纤维。最丰富的是嗜中温细菌，其次是细菌孢子和放线菌。织物遗存中最高可达107 CFU/g，埋葬遗存中最高可达5.2 × 106 CFU/g，棺材下最高可达1.6 × 106 CFU/g，而嗜干和非嗜干真菌最高可达7.5 × 105 CFU/g以上。NGS（新一代测序）结果表明，微生物的低存在可能是由于分枝杆菌中不可培养或长时间生长的细菌占主导地位，如M. coloregonium， M. arupense和M. pinnipedii。此外，其他强制性/非强制性病原体，脆弱拟杆菌、肉毒杆菌、伯纳氏杆菌、破伤风梭菌、白喉杆杆菌、阴沟肠杆菌、大肠杆菌、嗜肺军团菌、麻风分枝杆菌、马红球菌和金黄色葡萄球菌在检查样本中也有记录，并在骨骼样本中占主导地位。结果表明，历史文物中存在危险病原体的风险，对健康的影响可能严重，需要使用个人防护装备并采取适当措施控制地穴的物理状况。

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引用次数: 0

Rhizobium binxianense sp. nov. and Rhizobium mulingense sp. nov., isolated from nodules of Phaseolus vulgaris in Heilongjiang Province of China 从黑龙江省菜豆根瘤中分离的滨咸根瘤菌和mulingense根瘤菌

IF 3.3 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Systematic and applied microbiology

Pub Date : 2025-05-20 DOI: 10.1016/j.syapm.2025.126619

Yuxi Liu , Lili Liu , Ziqi Wang , Xiaoqian Huang , Yuxin Dong , Dong Hu , Chuntao Gu , En Tao Wang , Hao Wang

Rhizobial isolates from Phaseolus vulgaris (common bean) grown in Heilongjiang Province were grouped into two clusters based upon the phylogenies of 16S rRNA and recA-atpD genes representing by BJ04^T, MJ22, MJ37, BC56, MC62 and MC63^T, MJ21, MJ31, respectively. The phylogenetic analysis of whole genomes further revealed that BJ04^T, MJ22, MJ37, BC56 and MC62 formed a cluster neighbored Rhizobium chutanense C5^T, while MC63^T, MJ21 and MJ31 formed a cluster most related with Rhizobium croatiense 13T^T. The whole genome average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between the strains BJ04^T and R. chutanense C5^T and between MC63^T and R. croatiense 13T^T were lower than the species thresholds 95 % and 70 %, respectively. While the G + C content of the whole genome of the novel strains were 61.16–61.56 %, within the allowable GC content range of the Rhizobium genus (57–66 %). These results, combined with chemical classification and phenotype analysis, supported that BJ04^T and MC63^T represented two novel species and the names Rhizobium binxianense sp. nov. and Rhizobium mulingense sp. nov. were described for them. BJ04^T (=CCTCC AB 2022367^T, = JCM 35885^T) and MC63^T (=CCTCC AB 2024025^T, = JCM 36652^T) were designed as the type strains, respectively for these two novel species.

根据16S rRNA和recA-atpD基因（分别为BJ04T、MJ22、MJ37、BC56、MC62和MC63T、MJ21、MJ31）的系统发育特征，将黑龙江省菜豆根瘤菌分离物分为两类。全基因组系统发育分析进一步表明，BJ04T、MJ22、MJ37、BC56和MC62与chuutanense C5T形成一个集群，而MC63T、MJ21和MJ31与克罗地亚根瘤菌13TT形成一个集群。菌株BJ04T与楚坦纳河鼠C5T、MC63T与克罗地亚纳河鼠13TT的全基因组平均核苷酸同源性（ANI）和数字DNA-DNA杂交（dDDH）值分别低于种间阈值95%和70%。新菌株全基因组G + C含量为61.16 ~ 61.56%，在根瘤菌属允许的GC含量范围（57 ~ 66%）内。这些结果结合化学分类和表型分析，支持BJ04T和MC63T为两个新种，并为它们命名为binxianense sp. 11和mulingense sp. 11。将BJ04T （=CCTCC AB 2022367T, = JCM 35885T）和MC63T （=CCTCC AB 2024025T, = JCM 36652T）分别设计为这两个新种的型菌株。

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引用次数: 0

N-glyceroyl alkylamine phosphoglycolipids dominate the lipidome of several Bacillota bacteria n -甘油酰烷基胺磷酸糖脂在几种芽孢杆菌的脂质组中占主导地位

IF 3.3 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Systematic and applied microbiology

Pub Date : 2025-05-05 DOI: 10.1016/j.syapm.2025.126609

Nicole J. Bale , Michel Koenen , Su Ding , Jaap S. Sinninghe Damsté

Elucidation of the membrane lipid composition of bacteria can help to better understand how bacterial cells interact with their surroundings, adapt to environmental stress, and resist antimicrobial agents. Here we describe for the first time the detection of a wide array of N-glyceroyl alkylamine phosphoglycolipids (NGAPs) in a range of Bacillota bacteria (formerly Firmicutes). Bacillota includes a diverse range of bacteria that are typically highly resistant to harsh conditions such as heat, radiation, and pH, allowing the bacteria to survive in unfavorable environments. In 9 out 18 investigated strains of Bacillota, spread across 5 orders (Thermoanaerobacterales, Thermosediminibacterales, Eubacteriales, Halanaerobiales, and Sulfobacillia) mild acid hydrolysis released N-glyceroyl alkylamines (NGAs), which were detectable by gas chromatography–mass spectrometry (GC–MS) during routine fatty acid analysis. One strain, Moorella thermoacetica was found to produce long-chain NGAs (C₃₀-C₃₂), which are postulated to have isodiabolic acid-like structures. A wide variety of intact polar NGAPs were identified using ultra-high pressure liquid chromatography high resolution multi-stage mass spectrometry (UHPLC-HRMSⁿ). These include many previously undescribed lipids with a variety of sugar moieties and glycerol-bound core lipid moieties, including ether-bound components and alkyl 1,2-diols. The NGAPs constituted the majority of the intact polar lipid composition of these strains and presumably contribute to their tough cell membranes. The presence of NGAs in Bacillota appears to be associated with thermophilia. Both the hydrolysis-derived NGAs and intact polar NGAPs have potential to be biomarkers for extremophilic and, in particular, thermophilic bacteria.

阐明细菌的膜脂组成有助于更好地了解细菌细胞如何与周围环境相互作用，适应环境应激和抵抗抗菌剂。在这里，我们首次描述了在一系列杆菌门细菌（以前的厚壁菌门）中检测到广泛的n -甘油酰烷基胺磷酸糖脂（NGAPs）。杆状菌包括各种各样的细菌，这些细菌通常对高温、辐射和pH等恶劣条件具有很强的抵抗力，使细菌能够在不利的环境中生存。分布于5个目（热厌氧杆菌目、热沉积杆菌目、真细菌目、耐氧杆菌目和硫杆菌目）的18株芽孢杆菌中，有9株的温和酸水解释放出n -甘油酰烷基胺（NGAs），在常规脂肪酸分析中可通过气相色谱-质谱联用（GC-MS）检测到。一种菌株，热醋酸摩尔菌被发现产生长链NGAs (C30-C32)，被认为具有异二代谢酸样结构。采用超高压液相色谱-高分辨率多级质谱法（uhplc - hrms）鉴定了多种完整的极性ngap。这些包括许多以前描述过的具有各种糖部分和甘油结合的核心脂质部分的脂质，包括醚结合的成分和烷基1,2-二醇。NGAPs构成了这些菌株完整的极性脂质组成的大部分，可能有助于它们坚韧的细胞膜。芽孢杆菌中NGAs的存在似乎与嗜热性有关。水解衍生的ngap和完整的极性ngap都有可能成为嗜极细菌，特别是嗜热细菌的生物标志物。

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引用次数: 0

Corrigendum to: “Flavobacterium plantiphilum sp. nov., Flavobacterium rhizophilum sp. nov., Flavobacterium rhizosphaerae sp. nov., Chryseobacterium terrae sp. nov., and Sphingomonas plantiphila sp. nov. isolated from salty soil showing plant growth promoting potential” [Systematic and Applied Microbiology 48 (2025) 126588] 对“从盐渍土壤中分离出的植物生长促进潜力的植物黄杆菌、根状黄杆菌、根状黄杆菌、地黄杆菌和植鞘单胞菌”的更正[系统与应用微生物学48(2025)126588]。

IF 3.3 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Systematic and applied microbiology

Pub Date : 2025-05-01 DOI: 10.1016/j.syapm.2025.126603

Peter Kämpfer , André Lipski , Kathy S. Lawrence , Walker R. Olive , Molli M. Newman , John A. McInroy , Tomeu Viver

引用次数: 0

Erratum to “Characterization of two novel species of the genusBifidobacterium: Bifidobacterium saimiriisciurei sp. nov. and Bifidobacterium platyrrhinorum sp. nov.” [Syst. Appl. Microbiol. 43 (2020) 126111] “双歧杆菌属的两个新种：双歧杆菌和双歧杆菌的鉴定”的勘误(系统。达成。微生物学报，43(2020):126111。

IF 3.3 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Systematic and applied microbiology

Pub Date : 2025-05-01 DOI: 10.1016/j.syapm.2025.126601

Monica Modesto , Maria Satti , Koichi Watanabe , Donatella Scarafile , Chien-Hsun Huang , Jong-Shian Liou , Tomohiko Tamura , Satomi Saito , Mizuki Watanabe , Koji Mori , Lina Huang , Camillo Sandri , Caterina Spiezio , Masanori Arita , Paola Mattarelli

引用次数: 0