Pub Date : 2025-01-01DOI: 10.1016/j.syapm.2024.126562
Isabella Wilkie, Luis H. Orellana
Members of the phylum Verrucomicrobiota play a significant role in various ecosystems, yet they are underrepresented in databases due to their comparatively lower abundance and isolation challenges. The use of cultivation-independent approaches has unveiled their hidden diversity and specialized metabolic capabilities, yet many of these populations remain uncharacterized. In this study, we focus on members of the family MB11C04 associated with North Sea spring blooms. Our analyses revealed recurrent MB11C04 populations with increased abundance in the late stages of spring blooms over ten-years. By examining their genomic content, we identified specialized genetic features for the degradation of complex polysaccharides, particularly sulfated and fucose-rich compounds, suggesting their role in utilizing organic matter during the collapse of the bloom. Furthermore, we describe two novel genera each with a novel species (Seribacter gen. Nov., Chordibacter gen. Nov.) in accordance with the SeqCode initiative based on high quality metagenome-assembled genomes. We also propose a new name for the family MB11C04, Seribacteraceae. Our findings shed light on the ecological significance and metabolic potential of Verrucomicrobiota populations in spring bloom events.
Verrucomicrobiota门的成员在各种生态系统中发挥着重要作用，但由于其相对较低的丰度和分离挑战，它们在数据库中的代表性不足。利用不依赖于培养的方法揭示了它们隐藏的多样性和专门的代谢能力，但这些种群中的许多仍未被描述。在这项研究中，我们将重点放在与北海春季繁殖有关的MB11C04家族成员身上。我们的分析显示，在10年的时间里，MB11C04种群在春季开花后期的丰度增加。通过检查它们的基因组内容，我们确定了复杂多糖降解的特殊遗传特征，特别是硫酸盐和富含焦点的化合物，这表明它们在花崩期间利用有机物的作用。此外，根据基于高质量宏基因组组装基因组的SeqCode计划，我们描述了两个新属，每个属都有一个新种（Seribacter gen. Nov, Chordibacter gen. Nov）。我们还提出了一个新的名称MB11C04科，seriobacteraceae。我们的研究结果揭示了春季开花事件中Verrucomicrobiota种群的生态意义和代谢潜力。
{"title":"Elusive marine Verrucomicrobiota: Seasonally abundant members of the novel genera Seribacter and Chordibacter specialize in degrading sulfated glycans","authors":"Isabella Wilkie, Luis H. Orellana","doi":"10.1016/j.syapm.2024.126562","DOIUrl":"10.1016/j.syapm.2024.126562","url":null,"abstract":"<div><div>Members of the phylum <em>Verrucomicrobiota</em> play a significant role in various ecosystems, yet they are underrepresented in databases due to their comparatively lower abundance and isolation challenges. The use of cultivation-independent approaches has unveiled their hidden diversity and specialized metabolic capabilities, yet many of these populations remain uncharacterized. In this study, we focus on members of the family MB11C04 associated with North Sea spring blooms. Our analyses revealed recurrent MB11C04 populations with increased abundance in the late stages of spring blooms over ten-years. By examining their genomic content, we identified specialized genetic features for the degradation of complex polysaccharides, particularly sulfated and fucose-rich compounds, suggesting their role in utilizing organic matter during the collapse of the bloom. Furthermore, we describe two novel genera each with a novel species (<em>Seribacter</em> gen. Nov., <em>Chordibacter</em> gen. Nov.) in accordance with the SeqCode initiative based on high quality metagenome-assembled genomes. We also propose a new name for the family MB11C04, <em>Seribacteraceae</em>. Our findings shed light on the ecological significance and metabolic potential of <em>Verrucomicrobiota</em> populations in spring bloom events.</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 1","pages":"Article 126562"},"PeriodicalIF":3.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142787057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.syapm.2024.126577
Ya-Ling Mao , Xin-Yue Dong , Cong-Qi Tao, Zhang-Ping Wu, Xiao-Wei Shi, Jing Hou, Heng-Lin Cui
Four novel halophilic archaeal strains CGA53T, CG83T, FCH27T, and SEDH24 were isolated from a soda lake and two saline lakes in China, respectively. Strain CGA53T showed the highest 16S rRNA gene similarity (92.6%) to Salinilacihabitans rarus AD-4T, and the other three strains were found to be related to Halalkalicoccus species with similarities of 97.6–98.3%. Metagenomic studies indicated that these four strains are low abundant inhabitants detected in these hypersaline environments, and only one MAG of Chagannuoer Soda Lake (CG) could be assigned to the genus Halalkalicoccus. Their growth occurred at 20–60 °C (optima, 42, 37, 37–42, and 35 °C), 0.9–5.1 M NaCl (optima, 3.9, 2.6, 3.5, and 3 M), and 0–1.0 M MgCl2 (optima, 0.5, 0.7, and 0.1) and pH 5.5–10.5 (optima, 9.0, 7.5, 7.0, and 7.0), respectively. Phylogenetic and phylogenomic analyses revealed that strains CG83T, FCH27T, and SEDH24 cluster with the current species of the genus Halalkalicoccus, and strain CGA53T forms an independent branch separated from this genus. The average nucleotide identity (ANI), digital DNA–DNA hybridization (dDDH), and average amino acid identity (AAI) values among strains CGA53T, CG83T, FCH27T, SEDH24, and the type species of the current genera within the class Halobacteria were 67.4–81.6%, 16.5–28.6% and 49.7–74.1%, respectively, clearly lower than the cutoff values for species demarcation. Strain CGA53T may represent a novel species of a new genus according to the cutoff value for genus demarcation of 65% AAI. Diverse differential phenotypic characteristics, such as nutrition, biochemical activities, antibiotic sensitivity, and H2S formation, were found among these four strains and Halalkalicoccus species. Genome-based classification supported that strains CGA53T, CG83T, FCH27T, SEDH24, and the current species of Halalkalicoccus represent a novel family of the order Halobacteriales within the class Halobacteria.
从中国一个盐湖和两个盐湖分离到了4株新的嗜盐古菌CGA53T、CG83T、FCH27T和SEDH24。菌株CGA53T与Salinilacihabitans rarus AD-4T的16S rRNA基因相似性最高（92.6%），其余3株菌株与halalgicoccus属相关，相似度为97.6 ~ 98.3%。宏基因组学研究表明，这四种菌株在高盐环境中均为低丰度的居民，查加诺瓦苏打湖（CG）只有一种MAG可归属于嗜碱球菌属。它们分别在20-60℃（最优值，42、37、37-42和35℃）、0.9-5.1 M NaCl（最优值，3.9、2.6、3.5和3 M）和0-1.0 M MgCl2（最优值，0.5、0.7和0.1）和pH 5.5-10.5（最优值，9.0、7.5、7.0和7.0）条件下生长。系统发育和系统基因组分析表明，菌株CG83T、FCH27T和SEDH24与该属现有种聚集在一起，菌株CGA53T从该属中分离出来形成一个独立的分支。菌株CGA53T、CG83T、FCH27T、SEDH24和本属盐菌门类型种的平均核苷酸同源性（ANI）、数字DNA-DNA杂交（dDDH）和平均氨基酸同源性（AAI）值分别为67.4-81.6%、16.5-28.6%和49.7-74.1%，明显低于种划分的截断值。根据65% AAI的属划分截断值，菌株CGA53T可能代表一个新属的新种。在营养、生化活性、抗生素敏感性和H2S形成等表型差异特征方面，这4种菌株与嗜碱球菌存在差异。基于基因组的分类支持菌株CGA53T、CG83T、FCH27T、SEDH24和目前的hal碱球菌代表了盐杆菌纲中一个新的盐杆菌目家族。
{"title":"Natronorarus salvus gen. nov., sp. nov., Halalkalicoccus ordinarius sp. nov., and Halalkalicoccus salilacus sp. nov., halophilic archaea from a soda lake and two saline lakes, and proposal to classify the genera Halalkalicoccus and Natronorarus into Halalkalicoccaceae fam. nov. in the order Halobacteriales within the class Halobacteria","authors":"Ya-Ling Mao , Xin-Yue Dong , Cong-Qi Tao, Zhang-Ping Wu, Xiao-Wei Shi, Jing Hou, Heng-Lin Cui","doi":"10.1016/j.syapm.2024.126577","DOIUrl":"10.1016/j.syapm.2024.126577","url":null,"abstract":"<div><div>Four novel halophilic archaeal strains CGA53<sup>T</sup>, CG83<sup>T</sup>, FCH27<sup>T</sup>, and SEDH24 were isolated from a soda lake and two saline lakes in China, respectively. Strain CGA53<sup>T</sup> showed the highest 16S rRNA gene similarity (92.6%) to <em>Salinilacihabitans rarus</em> AD-4<sup>T</sup>, and the other three strains were found to be related to <em>Halalkalicoccus</em> species with similarities of 97.6–98.3%. Metagenomic studies indicated that these four strains are low abundant inhabitants detected in these hypersaline environments, and only one MAG of Chagannuoer Soda Lake (CG) could be assigned to the genus <em>Halalkalicoccus</em>. Their growth occurred at 20–60 °C (optima, 42, 37, 37–42, and 35 °C), 0.9–5.1 M NaCl (optima, 3.9, 2.6, 3.5, and 3 M), and 0–1.0 M MgCl<sub>2</sub> (optima, 0.5, 0.7, and 0.1) and pH 5.5–10.5 (optima, 9.0, 7.5, 7.0, and 7.0), respectively. Phylogenetic and phylogenomic analyses revealed that strains CG83<sup>T</sup>, FCH27<sup>T</sup>, and SEDH24 cluster with the current species of the genus <em>Halalkalicoccus</em>, and strain CGA53<sup>T</sup> forms an independent branch separated from this genus. The average nucleotide identity (ANI), digital DNA–DNA hybridization (dDDH), and average amino acid identity (AAI) values among strains CGA53<sup>T</sup>, CG83<sup>T</sup>, FCH27<sup>T</sup>, SEDH24, and the type species of the current genera within the class <em>Halobacteria</em> were 67.4–81.6%, 16.5–28.6% and 49.7–74.1%, respectively, clearly lower than the cutoff values for species demarcation. Strain CGA53<sup>T</sup> may represent a novel species of a new genus according to the cutoff value for genus demarcation of 65% AAI. Diverse differential phenotypic characteristics, such as nutrition, biochemical activities, antibiotic sensitivity, and H<sub>2</sub>S formation, were found among these four strains and <em>Halalkalicoccus</em> species. Genome-based classification supported that strains CGA53<sup>T</sup>, CG83<sup>T</sup>, FCH27<sup>T</sup>, SEDH24, and the current species of <em>Halalkalicoccus</em> represent a novel family of the order <em>Halobacteriales</em> within the class <em>Halobacteria</em>.</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 1","pages":"Article 126577"},"PeriodicalIF":3.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.syapm.2025.126579
M. Modesto , D. Scarafile , A. Vásquez , R. Pukall , M. Neumann-Schaal , S. Pascarelli , B. Sgorbati , M. Ancora , C. Cammà , P. Mattarelli , T.C. Olofsson
Six novel Bifidobacterium strains H1HS16NT, Bin2N, Hma3N, H6bp22N, H1HS10N, and H6bp9N, were isolated from the honey stomach of Apis mellifera. Cells are Gram-positive, non-motile, non-sporulating, facultatively anaerobic, and fructose 6-phosphate phosphoketolase-positive. Optimal growth conditions occur at 37 °C in anaerobiosis in MRS medium added with 2 % fructose and 0.1 % L-cysteine. The 16S rRNA gene sequences analysis revealed clustering with Bifidobacterium species found in honeybees. Strains Hma3N, H6bp22N, and H1HS16NT showed significant similarity to Bifidobacterium polysaccharolyticum JCM 34588T, with an average similarity of 99.63 %. In contrast, strains Bin2N, H1HS10N, and H6bp9N were closely related to Bifidobacterium apousia JCM 34587T, with an average similarity of 99.22 %. Moreover, strains Hma3N and H6bp22N exhibited ANI values of 96.65 % and 96.53 % when compared to Bifidobacterium polysaccharolyticum JCM 34588T, while strains H1HS16NT, Bin2N, H6bp9N, and H1HS10N revealed ANI values of 94.18 %, 94.33 %, 94.22 %, and 95.50 % respectively when compared to B. apousia JCM 34587T. dDDH analysis confirmed that strains Hma3N and H6bp22N belong to B. polysaccharolyticum, whereas strains H1HS16NT, Bin2N, H6bp9N, and H1HS10N represent a novel species. The peptidoglycan of the novel species is of the A4α type (L-Lys-D-Asp). The main cellular fatty acids of the type strain H1HS16NT are C16:0, C14:0, C19:0 cyclo ω9c, and C18:1 ω9c. The DNA G + C content of the type strain is 60.8 mol%.
Genome analyses of the strains were also conducted to determine their biosynthesis-related gene clusters, probiotic features, and ecological distribution patterns.
Phenotypic and genotypic characterization show that strain H1HS16NT is distinct from the type strains of other recognized Bifidobacterium species. Thus, Bifidobacterium kimbladii sp. nov. (H1HS16NT = DSM 115187T = CCUG 76695T) is proposed as a novel Bifidobacterium species.
{"title":"Phylogenetic characterization of Bifidobacterium kimbladii sp. nov., a novel species from the honey stomach of the honeybee Apis mellifera","authors":"M. Modesto , D. Scarafile , A. Vásquez , R. Pukall , M. Neumann-Schaal , S. Pascarelli , B. Sgorbati , M. Ancora , C. Cammà , P. Mattarelli , T.C. Olofsson","doi":"10.1016/j.syapm.2025.126579","DOIUrl":"10.1016/j.syapm.2025.126579","url":null,"abstract":"<div><div>Six novel <em>Bifidobacterium</em> strains H1HS16N<sup>T</sup>, Bin2N, Hma3N, H6bp22N, H1HS10N, and H6bp9N, were isolated from the honey stomach of <em>Apis mellifera</em>. Cells are Gram-positive, non-motile, non-sporulating, facultatively anaerobic, and fructose 6-phosphate phosphoketolase-positive. Optimal growth conditions occur at 37 °C in anaerobiosis in MRS medium added with 2 % fructose and 0.1 % L-cysteine. The 16S rRNA gene sequences analysis revealed clustering with <em>Bifidobacterium</em> species found in honeybees. Strains Hma3N, H6bp22N, and H1HS16N<sup>T</sup> showed significant similarity to <em>Bifidobacterium polysaccharolyticum</em> JCM 34588<sup>T</sup>, with an average similarity of 99.63 %. In contrast, strains Bin2N, H1HS10N, and H6bp9N were closely related to <em>Bifidobacterium apousia</em> JCM 34587<sup>T</sup>, with an average similarity of 99.22 %. Moreover, strains Hma3N and H6bp22N exhibited ANI values of 96.65 % and 96.53 % when compared to <em>Bifidobacterium polysaccharolyticum</em> JCM 34588<sup>T</sup>, while strains H1HS16N<sup>T</sup>, Bin2N, H6bp9N, and H1HS10N revealed ANI values of 94.18 %, 94.33 %, 94.22 %, and 95.50 % respectively when compared to <em>B. apousia</em> JCM 34587<sup>T</sup>. dDDH analysis confirmed that strains Hma3N and H6bp22N belong to <em>B. polysaccharolyticum</em>, whereas strains H1HS16N<sup>T</sup>, Bin2N, H6bp9N, and H1HS10N represent a novel species. The peptidoglycan of the novel species is of the A4α type (L-Lys-D-Asp). The main cellular fatty acids of the type strain H1HS16N<sup>T</sup> are C<sub>16:0</sub>, C<sub>14:0</sub>, C<sub>19:0</sub> cyclo ω9c, and C<sub>18:1</sub> ω9c. The DNA G + C content of the type strain is 60.8 mol%.</div><div>Genome analyses of the strains were also conducted to determine their biosynthesis-related gene clusters, probiotic features, and ecological distribution patterns.</div><div>Phenotypic and genotypic characterization show that strain H1HS16N<sup>T</sup> is distinct from the type strains of other recognized <em>Bifidobacterium</em> species. Thus, <em>Bifidobacterium kimbladii</em> sp. nov. (H1HS16N<sup>T</sup> = DSM 115187<sup>T</sup> = CCUG 76695<sup>T</sup>) is proposed as a novel <em>Bifidobacterium</em> species.</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 1","pages":"Article 126579"},"PeriodicalIF":3.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.syapm.2024.126576
Wei He , Dao-Feng Zhang , Xing-Jie Li , Hong-Chuan Wang , Lin-Qiong Wang , Yang Yuan
Four Gram-stain-negative, aerobic, rod-shaped bacteria, designated WL0002T, WL0024T, WL0050T, and WL0075T, were isolated from sediment in the coastal areas of Nantong City, China. Metagenomic analysis revealed higher relative abundance of taxa closely related to the four strains in sediment (0.79–2.0 %) than in water (0.34–1.3 %) (Mann-Whitney U test: p < 0.001). Phylogenetic analysis based on 16S rRNA gene and the bac120 gene set both suggested that the four strains are closely related to the genus Defluviimonas. Additionally, Albidovulum inexpectatum DSM 12048T formed a distinct branch within Defluviimonas. The evolutionary distance (ED) and percentage of conserved proteins (POCP) analysis indicated that the four strains and the genus strains of Albidovulum and Defluviimonas should be recognized as a single genus. Genomic relatedness analysis among the four strains and type strains of the genera Albidovulum and Defluviimonas was below species delimitation thresholds, except for strains WL0024T and “D. salinarum” CAU 1641T, which should belong to the same species. Based on phenotypic and genotypic characterization, the four strains should be recognized as novel species in Albidovulum, and it is reasonable to reclassify the genus Defluviimonas as a later heterotypic synonym of Albidovulum, consistent with the classification of the Genome Taxonomy Database (GTDB). Four names are proposed as follows: Albidovulum marisflavi sp. nov. (type strain WL0002T = MCCC 1K06013T = JCM 34653T = GDMCC 1.2437T), Albidovulum salinarum sp. nov. (WL0024T = MCCC 1K06062T = JCM 34656T = GDMCC 1.2438T), Albidovulum litorale sp. nov. (WL0050T = MCCC 1K07524T = JCM 35566T = GDMCC 1.3084T), and Albidovulum sediminicola sp. nov. (WL0075T = MCCC 1K06064T = JCM 34660T = GDMCC 1.2419T).
{"title":"Description of Albidovulum litorale sp. nov., Albidovulum marisflavi sp. nov., Albidovulum salinarum sp. nov., and Albidovulum sediminicola sp. nov., and proposal for reclassification of the genus Defluviimonas as a later heterotypic synonym of Albidovulum","authors":"Wei He , Dao-Feng Zhang , Xing-Jie Li , Hong-Chuan Wang , Lin-Qiong Wang , Yang Yuan","doi":"10.1016/j.syapm.2024.126576","DOIUrl":"10.1016/j.syapm.2024.126576","url":null,"abstract":"<div><div>Four Gram-stain-negative, aerobic, rod-shaped bacteria, designated WL0002<sup>T</sup>, WL0024<sup>T</sup>, WL0050<sup>T</sup>, and WL0075<sup>T</sup>, were isolated from sediment in the coastal areas of Nantong City, China. Metagenomic analysis revealed higher relative abundance of taxa closely related to the four strains in sediment (0.79–2.0 %) than in water (0.34–1.3 %) (Mann-Whitney <em>U</em> test: <em>p</em> < 0.001). Phylogenetic analysis based on 16S rRNA gene and the bac120 gene set both suggested that the four strains are closely related to the genus <em>Defluviimonas</em>. Additionally, <em>Albidovulum inexpectatum</em> DSM 12048<sup>T</sup> formed a distinct branch within <em>Defluviimonas</em>. The evolutionary distance (ED) and percentage of conserved proteins (POCP) analysis indicated that the four strains and the genus strains of <em>Albidovulum</em> and <em>Defluviimonas</em> should be recognized as a single genus. Genomic relatedness analysis among the four strains and type strains of the genera <em>Albidovulum</em> and <em>Defluviimonas</em> was below species delimitation thresholds, except for strains WL0024<sup>T</sup> and “<em>D. salinarum</em>” CAU 1641<sup>T</sup>, which should belong to the same species. Based on phenotypic and genotypic characterization, the four strains should be recognized as novel species in <em>Albidovulum</em>, and it is reasonable to reclassify the genus <em>Defluviimonas</em> as a later heterotypic synonym of <em>Albidovulum</em>, consistent with the classification of the Genome Taxonomy Database (GTDB). Four names are proposed as follows: <em>Albidovulum marisflavi</em> sp. nov. (type strain WL0002<sup>T</sup> = MCCC 1K06013<sup>T</sup> = JCM 34653<sup>T</sup> = GDMCC 1.2437<sup>T</sup>), <em>Albidovulum salinarum</em> sp. nov. (WL0024<sup>T</sup> = MCCC 1K06062<sup>T</sup> = JCM 34656<sup>T</sup> = GDMCC 1.2438<sup>T</sup>), <em>Albidovulum litorale</em> sp. nov. (WL0050<sup>T</sup> = MCCC 1K07524<sup>T</sup> = JCM 35566<sup>T</sup> = GDMCC 1.3084<sup>T</sup>), and <em>Albidovulum sediminicola</em> sp. nov. (WL0075<sup>T</sup> = MCCC 1K06064<sup>T</sup> = JCM 34660<sup>T</sup> = GDMCC 1.2419<sup>T</sup>).</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 1","pages":"Article 126576"},"PeriodicalIF":3.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142819250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-22DOI: 10.1016/j.syapm.2024.126565
Victoria A. Saltykova , Olga V. Danilova , Igor Y. Oshkin , Svetlana E. Belova , Natalia E. Suzina , Nikolai V. Pimenov , Svetlana N. Dedysh
A morphologically conspicuous microbial association was detected in a bioreactor running in a continuous mode with methanotrophic bacteria of the genus Methylococcus and natural gas as a growth substrate. The association consisted of spherical Methylococcus cells colonized by elongated rods, which produced rosette-like aggregates and inhibited the cultivation process. An isolate of these bacteria, strain S20T, was obtained and identified as belonging to the alphaproteobacterial family Ancalomicrobiaceae but displaying only a distant relationship (93.9–95.1 % 16S rRNA gene sequence similarity) to characterized members of this family. Strain S20T was represented by aerobic, motile, facultatively methylotrophic bacteria, which grew between 10 and 45 °C (optimum 30–35 °C) in a pH range of 4.5–8.5 (optimum pH 6.0). These bacteria were capable of attaching to Methylococcus cells and breaking the integrity of methanotroph cell walls, presumably to feed on methanol. The same interaction was observed with Methylomonas species. The finished genome sequence of strain S20T consisted of a 5.0 Mb chromosome and one plasmid, 0.26 Mb in size; the DNA G + C content was 68.4 %. The genome encoded 3 rRNA operons and ~ 4400 proteins including MxaFI- and XoxF-like methanol dehydrogenases, all enzymes of the serine pathway as well as a complete chemotaxis pathway, a unipolar polysaccharide adhesin, and a wide range of peptidases. The genome sequence displayed 67.20–69.56 % average amino acid identity to those of earlier described Ancalomicrobiaceae species. We propose to classify these bacteria as representing a novel genus and species, Methyloraptor flagellatus gen. Nov., sp. nov., with the type strain S20T (=KCTC 8649T = VKM B-3853T).
{"title":"Methyloraptor flagellatus gen. Nov., sp. nov., novel Ancalomicrobiaceae-affiliated facultatively methylotrophic bacteria that feed on methanotrophs of the genus Methylococcus","authors":"Victoria A. Saltykova , Olga V. Danilova , Igor Y. Oshkin , Svetlana E. Belova , Natalia E. Suzina , Nikolai V. Pimenov , Svetlana N. Dedysh","doi":"10.1016/j.syapm.2024.126565","DOIUrl":"10.1016/j.syapm.2024.126565","url":null,"abstract":"<div><div>A morphologically conspicuous microbial association was detected in a bioreactor running in a continuous mode with methanotrophic bacteria of the genus <em>Methylococcus</em> and natural gas as a growth substrate. The association consisted of spherical <em>Methylococcus</em> cells colonized by elongated rods, which produced rosette-like aggregates and inhibited the cultivation process. An isolate of these bacteria, strain S20<sup>T</sup>, was obtained and identified as belonging to the alphaproteobacterial family <em>Ancalomicrobiaceae</em> but displaying only a distant relationship (93.9–95.1 % 16S rRNA gene sequence similarity) to characterized members of this family. Strain S20<sup>T</sup> was represented by aerobic, motile, facultatively methylotrophic bacteria, which grew between 10 and 45 °C (optimum 30–35 °C) in a pH range of 4.5–8.5 (optimum pH 6.0). These bacteria were capable of attaching to <em>Methylococcus</em> cells and breaking the integrity of methanotroph cell walls, presumably to feed on methanol. The same interaction was observed with <em>Methylomonas</em> species. The finished genome sequence of strain S20<sup>T</sup> consisted of a 5.0 Mb chromosome and one plasmid, 0.26 Mb in size; the DNA G + C content was 68.4 %. The genome encoded 3 rRNA operons and ~ 4400 proteins including MxaFI- and XoxF-like methanol dehydrogenases, all enzymes of the serine pathway as well as a complete chemotaxis pathway, a unipolar polysaccharide adhesin, and a wide range of peptidases. The genome sequence displayed 67.20–69.56 % average amino acid identity to those of earlier described <em>Ancalomicrobiaceae</em> species. We propose to classify these bacteria as representing a novel genus and species, <em>Methyloraptor flagellatus</em> gen. Nov., sp. nov., with the type strain S20<sup>T</sup> (=KCTC 8649<sup>T</sup> = VKM B-3853<sup>T</sup>).</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 1","pages":"Article 126565"},"PeriodicalIF":3.3,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142700550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-21DOI: 10.1016/j.syapm.2024.126564
Yu-Ting OuYang , Le-Bin Chen , Jian-Yu Jiao , Zi-Xuan Hu , Jiong-Shan Wang , Tian-Hang Yang , Jun Xiao , Ting-Ting She , Pin-Jiao Jin , Shuang Wang , Lan Liu , Wen-Jun Li
Two siderophore-synthesizing species SYSU BS000078T and SYSU BS000231T were isolated from the black soil collected from fields located in Heilongjiang province, China. The results of phylogenetic analysis based on the 16S rRNA gene sequences indicated that these two strains showed the highest sequence similarity to Parafrigoribacterium mesophilum KCTC 19311T (98.61 % and 98.68 %, respectively). The average nucleotide identity (ANI) values between the two strains and other members of the genus Parafrigoribacterium were lower than 95 %, recommended for distinguishing novel prokaryotic species. Cells of strains SYSU BS000078T and SYSU BS000231T were aerobic, motile, Gram-stain-positive and non-spore-forming rods. The colonies of these two strains exhibited a cream pigment, with tidy edges and smooth surfaces. Growth was observed within the temperature range of 4–37 °C (optimal growth at 28 °C) and pH range of 6.0–8.0 (optimal growth at pH 7.0). The predominant polar lipids detected in these two strains included diphosphatidylglycerol and phosphatidylglycerol. The predominant respiratory quinones was MK-9. The major cellular fatty acids (>10 %) were iso-C16:0, anteiso-C15:0, and anteiso-C17:0. The genome size and G + C content of strain SYSU BS000078T were determined to be 2.89 Mbp and 66.5 %, respectively. Meanwhile, SYSU BS000231T exhibited a genome size of 2.81 Mbp, accompanied by a G + C content of 65.8 %. Based on the phenotypic, physiological, genotypic, and phylogenetic data, these two strains represent two novel species of the genus Parafrigoribacterium, which are proposed as Parafrigoribacterium soli sp. nov. SYSU BS000078T (=GDMCC 1.4599T = KCTC 59245T), and Parafrigoribacterium humi sp. nov. SYSU BS000231T (=GDMCC 1.3816T = KCTC 59001T).
{"title":"Parafrigoribacterium soli sp. nov. and Parafrigoribacterium humi sp. nov., two novel siderophore-synthesizing species isolated from black soil","authors":"Yu-Ting OuYang , Le-Bin Chen , Jian-Yu Jiao , Zi-Xuan Hu , Jiong-Shan Wang , Tian-Hang Yang , Jun Xiao , Ting-Ting She , Pin-Jiao Jin , Shuang Wang , Lan Liu , Wen-Jun Li","doi":"10.1016/j.syapm.2024.126564","DOIUrl":"10.1016/j.syapm.2024.126564","url":null,"abstract":"<div><div>Two siderophore-synthesizing species SYSU BS000078<sup>T</sup> and SYSU BS000231<sup>T</sup> were isolated from the black soil collected from fields located in Heilongjiang province, China. The results of phylogenetic analysis based on the 16S rRNA gene sequences indicated that these two strains showed the highest sequence similarity to <em>Parafrigoribacterium mesophilum</em> KCTC 19311<sup>T</sup> (98.61 % and 98.68 %, respectively). The average nucleotide identity (ANI) values between the two strains and other members of the genus <em>Parafrigoribacterium</em> were lower than 95 %, recommended for distinguishing novel prokaryotic species. Cells of strains SYSU BS000078<sup>T</sup> and SYSU BS000231<sup>T</sup> were aerobic, motile, Gram-stain-positive and non-spore-forming rods. The colonies of these two strains exhibited a cream pigment, with tidy edges and smooth surfaces. Growth was observed within the temperature range of 4–37 °C (optimal growth at 28 °C) and pH range of 6.0–8.0 (optimal growth at pH 7.0). The predominant polar lipids detected in these two strains included diphosphatidylglycerol and phosphatidylglycerol. The predominant respiratory quinones was MK-9. The major cellular fatty acids (>10 %) were iso-C<sub>16:0</sub>, anteiso-C<sub>15:0</sub>, and anteiso-C<sub>17:0</sub>. The genome size and G + C content of strain SYSU BS000078<sup>T</sup> were determined to be 2.89 Mbp and 66.5 %, respectively. Meanwhile, SYSU BS000231<sup>T</sup> exhibited a genome size of 2.81 Mbp, accompanied by a G + C content of 65.8 %. Based on the phenotypic, physiological, genotypic, and phylogenetic data, these two strains represent two novel species of the genus <em>Parafrigoribacterium</em>, which are proposed as <em>Parafrigoribacterium soli</em> sp. nov. SYSU BS000078<sup>T</sup> (=GDMCC 1.4599<sup>T</sup> = KCTC 59245<sup>T</sup>), and <em>Parafrigoribacterium humi</em> sp. nov. SYSU BS000231<sup>T</sup> (=GDMCC 1.3816<sup>T</sup> = KCTC 59001<sup>T</sup>).</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 1","pages":"Article 126564"},"PeriodicalIF":3.3,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142744184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-20DOI: 10.1016/j.syapm.2024.126563
Feng-Bai Lian , Bing-Jun Zhou , Zi-Yang Zhou , Alejandro P. Rooney , Zhen-Xing Xu , Zong-Jun Du
The family Woeseiaceae, also known as the JTB255 bacterial group, are ubiquitous and abundant core members of microbial communities in marine surface sediments. However, to date, only one Woeseiaceae strain isolated from marine sediments has been described, and the phylogeny and environmental adaptation mechanisms of this group have been little explored. Here, we isolated five novel Woeseiaceae strains from the marine solar saltern in Weihai, China. Multiple genomic, physiological, and chemotaxonomic characteristics supported that these five isolates represent three novel species within a novel genus, for which Lentisalinibacter gen. nov. and three species Lentisalinibacter sediminis sp. nov., Lentisalinibacter salinarum sp. nov. and Lentisalinibacter orientalis sp. nov. are proposed. Moreover, phylogenetic analysis based on the 16S rRNA genes and genome sequences revealed that Woeseiaceae is most closely related to Steroidobacterales. Further comparative genomics analysis indicated the separate evolution of Woeseiaceae and Steroidobacterales, supporting the emended description of the order Woeseiales. Woeseiales representitives showed facultatively anaerobic characteristics and small genome sizes in contrast to their phylogenetic relatives. They primarily inhabit surface marine sediment environments using multiple metabolic and ecological strategies to adapt to the changing microenvironments. Our results demonstrate the novel representatives of Woeseiales and their environmental adaptation mechanisms in marine environments.
{"title":"Describing five new strains in the family Woeseiaceae and emended description of the order Woeseiales with genomic features related to environmental adaptation","authors":"Feng-Bai Lian , Bing-Jun Zhou , Zi-Yang Zhou , Alejandro P. Rooney , Zhen-Xing Xu , Zong-Jun Du","doi":"10.1016/j.syapm.2024.126563","DOIUrl":"10.1016/j.syapm.2024.126563","url":null,"abstract":"<div><div>The family <em>Woeseiaceae</em>, also known as the JTB255 bacterial group, are ubiquitous and abundant core members of microbial communities in marine surface sediments. However, to date, only one <em>Woeseiaceae</em> strain isolated from marine sediments has been described, and the phylogeny and environmental adaptation mechanisms of this group have been little explored. Here, we isolated five novel <em>Woeseiaceae</em> strains from the marine solar saltern in Weihai, China. Multiple genomic, physiological, and chemotaxonomic characteristics supported that these five isolates represent three novel species within a novel genus, for which <em>Lentisalinibacter</em> gen. nov. and three species <em>Lentisalinibacter sediminis</em> sp. nov., <em>Lentisalinibacter salinarum</em> sp. nov. and <em>Lentisalinibacter orientalis</em> sp. nov. are proposed. Moreover, phylogenetic analysis based on the 16S rRNA genes and genome sequences revealed that <em>Woeseiaceae</em> is most closely related to <em>Steroidobacterales</em>. Further comparative genomics analysis indicated the separate evolution of <em>Woeseiaceae</em> and <em>Steroidobacterales</em>, supporting the emended description of the order <em>Woeseiales</em>. <em>Woeseiales</em> representitives showed facultatively anaerobic characteristics and small genome sizes in contrast to their phylogenetic relatives. They primarily inhabit surface marine sediment environments using multiple metabolic and ecological strategies to adapt to the changing microenvironments. Our results demonstrate the novel representatives of <em>Woeseiales</em> and their environmental adaptation mechanisms in marine environments.</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 1","pages":"Article 126563"},"PeriodicalIF":3.3,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142700552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1016/j.syapm.2024.126561
Anastasia I. Maltseva , Alexander G. Elcheninov , Alexandra A. Klyukina , Nikolay V. Pimenov , Andrei A. Novikov , Alexander V. Lebedinsky , Evgenii N. Frolov
Representatives of the genus Thermodesulfovibrio are widespread thermophilic sulfate-reducing bacteria. The genus currently includes five species with validly published names. Two new Thermodesulfovibrio strains, 3907-1M T and 3462-1T, were isolated with molecular hydrogen as an electron donor, sulfate as an electron acceptor and acetate as the carbon source from hot springs of Kunashir Island and Kamchatka Peninsula. Similar to other Thermodesulfovibrio species, the new isolates grew by reduction of sulfate, thiosulfate or Fe (III) with a limited range of electron donors, such as hydrogen (in the presence of acetate), formate (in the presence of acetate), pyruvate and lactate. Surprisingly, strain 3907-1MT proved to be capable of autotrophic growth as well. Up to now, the genus Thermodesulfovibrio was represented by heterotrophic species only. Genome analysis revealed the presence of a gene cluster encoding enzymes of form III RubisCO-mediated transaldolase variant of the Calvin cycle in both strains, but genes encoding ribulose-1,5-bisphosphate carboxylase and phosphoribulokinase in the genome of the strain 3462-1T contained internal stop codons in their sequences. On the basis of phylogenomic analysis, as well as distinct phenotypic and genomic properties, strain 3907-1MT (=DSM 112797T =JCM 39445T =VKM B-3594T =UQM 41601T) is proposed to be classified as Thermodesulfovibrio autotrophicus sp. nov., and strain 3462-1T (=JCM 39444T =VKM B-3714T =UQM 41602T) – as Thermodesulfovibrio obliviosus sp. nov. Our results demonstrate a chemolithoautotrophic lifestyle in Thermodesulfovibrio representatives, suggesting greater ecological flexibility of this genus than previously assumed.
{"title":"Thermodesulfovibrio autotrophicus sp. nov., the first autotrophic representative of the widespread sulfate-reducing genus Thermodesulfovibrio, and Thermodesulfovibrio obliviosus sp. nov. that has lost this ability","authors":"Anastasia I. Maltseva , Alexander G. Elcheninov , Alexandra A. Klyukina , Nikolay V. Pimenov , Andrei A. Novikov , Alexander V. Lebedinsky , Evgenii N. Frolov","doi":"10.1016/j.syapm.2024.126561","DOIUrl":"10.1016/j.syapm.2024.126561","url":null,"abstract":"<div><div>Representatives of the genus <em>Thermodesulfovibrio</em> are widespread thermophilic sulfate-reducing bacteria. The genus currently includes five species with validly published names. Two new <em>Thermodesulfovibrio</em> strains, 3907-1M <sup>T</sup> and 3462-1<sup>T</sup>, were isolated with molecular hydrogen as an electron donor, sulfate as an electron acceptor and acetate as the carbon source from hot springs of Kunashir Island and Kamchatka Peninsula. Similar to other <em>Thermodesulfovibrio</em> species, the new isolates grew by reduction of sulfate, thiosulfate or Fe (III) with a limited range of electron donors, such as hydrogen (in the presence of acetate), formate (in the presence of acetate), pyruvate and lactate. Surprisingly, strain 3907-1M<sup>T</sup> proved to be capable of autotrophic growth as well. Up to now, the genus <em>Thermodesulfovibrio</em> was represented by heterotrophic species only. Genome analysis revealed the presence of a gene cluster encoding enzymes of form III RubisCO-mediated transaldolase variant of the Calvin cycle in both strains, but genes encoding ribulose-1,5-bisphosphate carboxylase and phosphoribulokinase in the genome of the strain 3462-1<sup>T</sup> contained internal stop codons in their sequences. On the basis of phylogenomic analysis, as well as distinct phenotypic and genomic properties, strain 3907-1M<sup>T</sup> (=DSM 112797<sup>T</sup> =JCM 39445<sup>T</sup> =VKM B-3594<sup>T</sup> =UQM 41601<sup>T</sup>) is proposed to be classified as <em>Thermodesulfovibrio autotrophicus</em> sp. nov., and strain 3462-1<sup>T</sup> (=JCM 39444<sup>T</sup> =VKM B-3714<sup>T</sup> =UQM 41602<sup>T</sup>) – as <em>Thermodesulfovibrio obliviosus</em> sp. nov. Our results demonstrate a chemolithoautotrophic lifestyle in <em>Thermodesulfovibrio</em> representatives, suggesting greater ecological flexibility of this genus than previously assumed.</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"47 6","pages":"Article 126561"},"PeriodicalIF":3.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1016/j.syapm.2024.126560
Violetta Shestivska , Petra Španělová , Marcela Krůtová , Martina Maixnerová , Priscila Thiago Dobbler , Tomáš Větrovský , Alexandr Nemec , Martina Kyselková
This study aimed to determine the taxonomic status of a new group of bovine strains of the genus Acinetobacter characterized by the rare ability to grow at temperatures above 44 °C. Initially, 24 strains were isolated from cattle feces collected at 11 farms in Czechia in 2022, representing a tentative new species based on preliminary whole-cell MALDI-TOF MS identification and rpoB gene sequencing. Twelve strains encompassing the within-group diversity were studied in detail, including whole genome de novo sequencing. Core genome-based phylogenetic analysis revealed that the 12 strains form a distinct clade within the genus. The genomic average nucleotide identity based on BLAST (ANIb) values within the clade were ≥ 98.2%, whereas the ANIb values between the clade and the known Acinetobacter species were < 83%. Each of the 12 genomes consisted of a circular chromosome (3.05−3.28 Mb) and 1−6 extrachromosomal elements (1.43−122.5 kb). All strains were non-glucose oxidizing, non-hemolytic, non-proteolytic, and prototrophic, and used acetate, ethanol, and DL-lactate as sole carbon and energy sources. Unrestricted growth at 47 °C was their unique diagnostic characteristic in a genus-wide comparative analysis and their growth kinetics at 37–46 °C differed from that of Acinetobacter baumannii. The studied strains were susceptible to 18 antibiotics except for sporadic resistance to streptomycin, tetracycline, and sulfonamides associated with the presence of strA/strB, tet(Y), and sul2 genes, respectively. We conclude that the studied strains represent a new species, for which we propose the name Acinetobacter thermotolerans sp. nov. The type strain is ANC 7454T (= CCM 9356T = CCUG 77195T = CNCTC 8200T).
{"title":"Proposal of Acinetobacter thermotolerans sp. nov. to accommodate bovine feces-dwelling bacteria growing at 47 °C","authors":"Violetta Shestivska , Petra Španělová , Marcela Krůtová , Martina Maixnerová , Priscila Thiago Dobbler , Tomáš Větrovský , Alexandr Nemec , Martina Kyselková","doi":"10.1016/j.syapm.2024.126560","DOIUrl":"10.1016/j.syapm.2024.126560","url":null,"abstract":"<div><div>This study aimed to determine the taxonomic status of a new group of bovine strains of the genus <em>Acinetobacter</em> characterized by the rare ability to grow at temperatures above 44 °C. Initially, 24 strains were isolated from cattle feces collected at 11 farms in Czechia in 2022, representing a tentative new species based on preliminary whole-cell MALDI-TOF MS identification and <em>rpoB</em> gene sequencing. Twelve strains encompassing the within-group diversity were studied in detail, including whole genome <em>de novo</em> sequencing. Core genome-based phylogenetic analysis revealed that the 12 strains form a distinct clade within the genus. The genomic average nucleotide identity based on BLAST (ANIb) values within the clade were ≥ 98.2%, whereas the ANIb values between the clade and the known <em>Acinetobacter</em> species were < 83%. Each of the 12 genomes consisted of a circular chromosome (3.05−3.28 Mb) and 1−6 extrachromosomal elements (1.43−122.5 kb). All strains were non-glucose oxidizing, non-hemolytic, non-proteolytic, and prototrophic, and used acetate, ethanol, and DL-lactate as sole carbon and energy sources. Unrestricted growth at 47 °C was their unique diagnostic characteristic in a genus-wide comparative analysis and their growth kinetics at 37–46 °C differed from that of <em>Acinetobacter baumannii</em>. The studied strains were susceptible to 18 antibiotics except for sporadic resistance to streptomycin, tetracycline, and sulfonamides associated with the presence of <em>strA</em>/<em>strB</em>, <em>tet</em>(Y), and <em>sul2</em> genes, respectively. We conclude that the studied strains represent a new species, for which we propose the name <em>Acinetobacter thermotolerans</em> sp. nov. The type strain is ANC 7454<sup>T</sup> (= CCM 9356<sup>T</sup> = CCUG 77195<sup>T</sup> = CNCTC 8200<sup>T</sup>).</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"47 6","pages":"Article 126560"},"PeriodicalIF":3.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1016/j.syapm.2024.126558
Chun Tao Gu
{"title":"Corrigendum to “Characterization of two novel pentose-fermenting and GABA-producing species: Levilactobacillus tujiorum sp. nov. and Secundilactobacillus angelensis sp. nov. Isolated from a solid-state fermented zha-chili” [Syst. Appl. Microbiol. 45(5) (2022) 126344]","authors":"Chun Tao Gu","doi":"10.1016/j.syapm.2024.126558","DOIUrl":"10.1016/j.syapm.2024.126558","url":null,"abstract":"","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"47 6","pages":"Article 126558"},"PeriodicalIF":3.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142508428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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