Sustainable Environment Research最新文献

3区环境科学与生态学 Q1 Environmental Science

Sustainable Environment Research

Pub Date : 2023-09-12 DOI: 10.1186/s42834-023-00191-w

Behnam Askari Lasaki, Peter Maurer, Harald Schönberger

引用次数: 0

Efficient degradation of various recalcitrant azo dyes in aqueous medium by immobilized Origanum vulgare peroxidase 固定化土豆花过氧化物酶在水介质中高效降解各种顽固性偶氮染料

3区环境科学与生态学 Q1 Environmental Science

Sustainable Environment Research

Pub Date : 2023-09-12 DOI: 10.1186/s42834-023-00190-x

Mahsa Golbabaie, Behzad Gharahchei, Fatemeh Mirazizi, Alireza Abbasi Baharanchi, Ali Khosronejad, Ali Asghar Karkhanie, Kamahldin Haghbeen

Abstract Hybrid xerogels, constructed from organic and inorganic silanes, have emerged as materials with versatile applications. They have shown promising potential for immobilization as their chemical structures encompass the mechanical, thermal, and structural stability of inorganic compounds in synergy with the functionality and flexibility of organic compounds. Incorporating a magnetic core and preparation at the nanoscale multiply their attraction for enzyme immobilization. To evaluate how efficiently this technology works for the immobilization of a plant peroxidase, a novel peroxidase (POX ov ) from a famous medicinal herb, Origanum vulgare , was immobilized on magnetic nanoparticles of a hybrid xerogel obtained from tetraethyl orthosilicate and (3-aminopropyl)triethoxysilane. Immobilization boosted the physicochemical properties of the enzyme so that the immobilized POX ov (I.POX ov ) could tolerate lower pHs and higher temperatures. It oxidized all types of the examined phenolic, catecholic, guaiacolic, and aniline diazo dyes, while the free POX ov (F.POX ov ) failed to oxidize the last group. These improvements also made I.POX ov capable of oxidizing the recalcitrant azo dyes carrying electron-withdrawing groups much faster than F.POX ov . I.POX ov was employed in 7 successive cycles of oxidation of both phenolic and guaiacolic dyes with an average efficiency of 69%. The efficacy of the immobilization method and I.POXov competency for the enzymatic remediation of polluted water resources have been quantitatively demonstrated.

由有机硅烷和无机硅烷构成的杂化干凝胶已经成为一种具有多种用途的材料。由于其化学结构包含无机化合物的机械、热和结构稳定性，并与有机化合物的功能性和柔韧性协同作用，它们已显示出很好的固定化潜力。结合磁芯和纳米级的制备，增加了它们对酶固定的吸引力。为了评估该技术固定化植物过氧化物酶的效率，研究人员将一种来自著名草药Origanum vulgare的新型过氧化物酶(POX ov)固定在由正硅酸四乙酯和(3-氨基丙基)三乙氧基硅烷制备的杂化干凝胶的磁性纳米颗粒上。固定化提高了酶的理化性质，使固定化后的POX ov能够耐受较低的ph值和较高的温度。它可以氧化所有类型的酚类、儿茶酚类、愈创木酚类和苯胺类重氮染料，而游离的POX ov (F.POX ov)不能氧化最后一类染料。这些改进也使I.POX ov能够比F.POX ov更快地氧化带有吸电子基团的顽固偶氮染料。用1 . pox ov连续7个循环氧化酚类和愈创木酚类染料，平均效率为69%。固定化方法的有效性和poxov对污染水资源的酶修复能力已经得到了定量证明。

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引用次数: 0

Bio-green synthesis of calcium acetate from oyster shell waste at low cost and reducing the emission of greenhouse gases 低成本生物绿色合成牡蛎壳废物醋酸钙，减少温室气体排放