Rapid Communications in Mass Spectrometry最新文献

{"title":"Trace Determination of 16 Phenolic Endocrine-Disrupting Chemicals in Environmental Waters: A Validated SPE–UPLC–MS/MS Approach","authors":"Bao Song,&nbsp;Xinting Wang,&nbsp;Anchen Liu,&nbsp;Dongxue Lei,&nbsp;Yuelong Qin,&nbsp;Cui Pan,&nbsp;Yangzhao Sun,&nbsp;Shujun Zhong","doi":"10.1002/rcm.10155","DOIUrl":"10.1002/rcm.10155","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>Phenolic endocrine-disrupting chemicals (EDCs), including alkylphenols (APs) and bisphenols (BPs), pose significant environmental and public health concerns due to their widespread presence in aquatic ecosystems and potential toxicity at trace concentrations. This study developed and validated a sensitive analytical method for the simultaneous determination of 16 phenolic EDCs in environmental waters.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The method involves sample acidification to pH 2, hydrophilic–lipophilic balance (HLB), solid-phase extraction (SPE), and ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) analysis with negative electrospray ionization (ESI−). Systematic optimization revealed that an alkaline mobile phase (methanol/0.1% ammonia) significantly enhanced the ionization efficiency of phenolic compounds, improving sensitivity by approximately an order of magnitude compared to acidic conditions. Chromatographic separation was optimized using an ACQUITY UPLC BEH C₁₈ column, which provided superior peak shapes and improved retention of hydrophobic compounds.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Acidification of samples prior to SPE was critical for maximizing analyte recovery (57.9%–94.2%). The validated method demonstrated outstanding analytical performance, achieving low detection limits (0.01–0.4 ng/L), excellent linearity (<i>R</i> = 0.991–0.999) across a broad concentration range (1.0–50.0 μg/L) and high precision (RSDs = 1.3%–13.6%). Application of the method to environmental samples collected from rivers, wastewater treatment plants, and landfill leachates identified eight target compounds, with alkylphenols detected at higher concentrations than BPs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This reliable and robust method provides a valuable tool for monitoring trace levels of phenolic EDCs in diverse aquatic environments, supporting technically comprehensive environmental assessments and regulatory compliance.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"40 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacodynamic and Metabolomic Mechanism of Shexiang Baoxin Pill Based on db/db Mice on Treating Type 2 Diabetes Mellitus","authors":"Zifan Zhu,&nbsp;Bie Xie,&nbsp;Zhicong Wang,&nbsp;Lin Peng,&nbsp;Li Xiang,&nbsp;Runhui Liu","doi":"10.1002/rcm.10153","DOIUrl":"10.1002/rcm.10153","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>Shexiang Baoxin Pill (SBP) has been shown to assist in lowering blood glucose levels in patients with type 2 diabetes mellitus (T2DM) in clinical studies. However, the antidiabetic metabolism of SBP has not yet been fully elucidated.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Male <i>db/db</i> mice were used as an animal model of T2DM. Mice were given metformin (Met), SBP, and a combination of Met and SBP in low dosage (Met-SBP) and high dosage (Met-HSBP) via gavage for a duration of 8 weeks, respectively. Metabolomics analysis of serum samples was performed on ultra-high-performance liquid chromatography coupled with Orbitrap Exploris 120 mass spectrometry.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The animal experiment showed that SBP effectively assists in lowering fasting blood glucose levels and significantly attenuating insulin resistance in <i>db/db</i> mice. A total of 30 metabolites with significant changes were identified, mainly involving lipids, amino acids, and acylcarnitines. Among these regulated metabolites, 9 metabolites were affected by Met, 10 by SBP, 27 by Met-SBP, and 9 by Met-HSBP. The results revealed that SBP specifically regulated the dysfunction of medium-chain acylcarnitines in the fatty acid oxidation (FAO) pathway. Additionally, the combined use of SBP and Met may play a synergistic role in treating T2DM.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>SBP has a positive effect in regulating fasting glucose and insulin sensitivity in T2DM <i>db/db</i> mice, possibly through the FAO metabolic pathway. SBP in combination with Met may play a positive role in the inflammatory response. The results of this study may offer a theoretical foundation for the clinical application of SBP on T2DM and provide new insights for the research of traditional Chinese medicine in treating diseases.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"40 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145278561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of the Chemical Constituents in Viticis Fructus From Different Regions by UPLC-QTOF-MS Combined With Multivariate Statistical Analyses","authors":"Yuanyuan Zhang,&nbsp;Chengcheng Yuan,&nbsp;Qiuyun Wu,&nbsp;Yi Wu,&nbsp;Bin Huang,&nbsp;Wen Liu,&nbsp;Wenkai Lv,&nbsp;Chunxia Yu,&nbsp;Le Chen","doi":"10.1002/rcm.10144","DOIUrl":"10.1002/rcm.10144","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>Viticis fructus (VF) is a valuable traditional Chinese herbal medicine with high commercial value. In the Chinese Pharmacopoeia (Ch.P., 2020 edition), the quality of VF is mainly evaluated by its casticin content. However, varying growth and production conditions, such as cultivation environment, origin, and harvesting process, result in highly variable yields, quality, and composition. The aim of this study was to propose a new method for discriminating and quantifying VF from different regions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The chemical constituents of VF from different regions were collected by ultraperformance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) using electrospray ionization (ESI) in both positive and negative ion modes, and the chemical constituents were qualitatively analyzed by Waters UNIFI portal 1.9.4.0 software combined with the self-developed database of VF. The spectra obtained by Progenesis QI 27.26.1020 and EZinfo 2.0 software were processed by peak standardization, peak extraction, peak alignment, peak matching, and so forth, and the information of compound retention time and mass–charge ratio was obtained. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were performed by SIMCA-P 14.0 software to study the different components of VF from different regions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 102 components were identified from VF. The results of PCA and OPLS-DA showed that there were significant differences in the chemical components of VF from different regions. Twenty-seven different components were obtained by variable importance for the projection (VIP) &gt; 1 screening, including 12 terpenoids, 7 phenylpropyl compounds, 3 phenolic acids, 2 flavonoids, 2 fatty acids, and 1 steroid.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>By establishing a method of UPLC-QTOF-MS combined with multivariate statistical analyses, this study clarifies the difference in chemical components between VF from different regions, which provides a basis for the quality control of VF and provides basic information for revealing the impact of the ecological environment on the accumulation of compounds in VF.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"40 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Explosives Using the SICRIT Ionization Source Based on a Dielectric Barrier Discharge Coupled to a Linear Ion Trap Mass Spectrometer","authors":"Jay Clark,&nbsp;Jason V. Miller,&nbsp;Eshwar Jagerdeo","doi":"10.1002/rcm.10148","DOIUrl":"10.1002/rcm.10148","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>This paper evaluates the use of a plasma source coupled to an LTQ mass spectrometer for the analysis of explosive residues from soil samples, and in the process examines the advantages and limitations of this new ionization source.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Samples were solvent extracted, centrifuged, filtered, and injected into the Solid-Phase Microextraction (SPME)-like device that is coupled to the plasma ion source. The ion source is directly coupled to the orifice of the mass spectrometer, which allows for all ions generated to enter the mass spectrometer. Using the nitrate ion formed in the background and the introduction of ammonium chloride solution, the adduct formations with eight explosives (ETN, HMX, PETN, R-Salt, RDX, tetryl, MHN, and NG) were monitored.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Reagent ion formation under nitrogen and chloride conditions was studied to determine the optimal conditions for generating adducts with explosive analytes. Extracted soil samples with spiked explosives concentrations ranging from 5 to 1600 ng/mL were analyzed and monitored for the relevant nitrate and chloride adduct masses. For the formation of nitrate adducts, humidified nitrogen at 1500 V was determined to be better than dry nitrogen. For the formation of chloride adducts, 500 mM ammonium chloride at 1500 V was determined to be optimal.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The highest sensitivity can be achieved by directly coupling the plasma source to the orifice of the LTQ mass spectrometer. A maximum of 2 μL of sample can be introduced in this configuration, but the potential exists to modify the technique for larger volumes.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"40 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145237448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ecological Insights Into the Extinct Korean Sea Lion (Zalophus japonicus) in Korea Based on Stable Isotope Analysis of Bone Collagen","authors":"Yoon Ji Lee,&nbsp;Myung Joon Kim,&nbsp;Ji Seong Kim,&nbsp;Seok Nam Kwak,&nbsp;Sang Heon Lee","doi":"10.1002/rcm.10140","DOIUrl":"10.1002/rcm.10140","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>The Japanese sea lion (<i>Zalophus japonicus</i>), once abundant in Japan, Korea, and Russia, went extinct by the mid-20th century due to hunting and environmental change. This study examines the diet and ecological role of Korean <i>Z. japonicus</i> using stable carbon and nitrogen isotope analysis of bone collagen from archaeological and historical contexts.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Skeletal remains of the extinct Korean sea lion (<i>Z. japonicus</i>) were excavated from Gajae-gull, Ulleungdo, in 2021. A rib bone was analyzed for radiocarbon dating using AMS and calibrated with the Marine20 curve, applying a ΔR correction. Stable isotope analysis of extracted bone collagen (δ¹³C, δ¹⁵N) was performed via EA-IRMS. Isotopic niches of <i>Z. japonicus</i> and modern marine mammals were compared using SIBER in R, with ANOVA and post hoc tests.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Radiocarbon dating of a rib from Gajae-gull, Ulleungdo, indicated burial between 1548 and 1952 cal <span>ad</span>, representing the most recent evidence of <i>Z. japonicus</i> in Korea. Stable isotope analysis revealed dietary shifts from high δ¹⁵N values in the Chulmun periods, indicating consumption of large fish and cephalopods, to lower δ¹⁵N and higher δ¹³C values in the Mumun and historic periods, indicating reliance on coastal mid-trophic prey. Comparisons with modern marine mammals showed clear isotopic niche partitioning.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>These findings underscore the dietary flexibility of Korean <i>Z. japonicus</i> and its shifting ecological role in response to changing prey availability and environmental conditions. Future research incorporating additional samples and diverse isotopic markers will enhance paleoecological reconstructions of this extinct species and its historical marine ecosystems.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"39 24","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/rcm.10140","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultratrace Determination of Cd (II) in Water, Salt, Environmental, Alloy, and Biological Samples by Liquid–Liquid Extraction Followed by Inductively Coupled Plasma Mass Spectrometry Technique","authors":"Chandramauly Sharma,&nbsp;Kuldeep Sharma,&nbsp;Bignesh Thakur,&nbsp;Aniket Dixit","doi":"10.1002/rcm.10150","DOIUrl":"https://doi.org/10.1002/rcm.10150","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>Cadmium is a highly toxic heavy metal associated with severe environmental and health risks, making its precise quantification in complex matrices critical. Inductively coupled plasma mass spectrometry (ICP–MS) is one of the most powerful tools for trace metal analysis due to its exceptional sensitivity, selectivity, and multielement capability. However, accurate detection often requires effective preconcentration and matrix elimination strategies.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Method</h3>\u0000 \u0000 <p>A liquid–liquid extraction procedure was developed using N,N′-diphenyl sebacoyl dihydroxamic acid (DPSDHA) as a selective complexing ligand for cadmium. The Cd–DPSDHA binary complex formed at pH 9.5 was extracted into dichloromethane, effectively preconcentrating cadmium and minimizing matrix interference prior to ICP–MS measurement. The optimized conditions ensured maximum recovery and stability of the analyte, thereby improving detection limits and analytical precision.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Result</h3>\u0000 \u0000 <p>Coupling the DPSDHA extraction with ICP–MS enabled trace-level determination of cadmium with excellent sensitivity and reproducibility. The method exhibited a wide linear dynamic range, low detection limits, and strong resistance to matrix interferences, ensuring accurate quantification in complex samples. Application to water, alloy, environmental, and biological samples yielded accurate results, which were consistent with certified reference values.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The combination of selective DPSDHA-based extraction with ICP–MS provides a robust, highly sensitive approach for cadmium determination in diverse sample matrices. This methodology enhances detection accuracy by reducing matrix effects, making it well-suited for environmental monitoring, industrial quality control, and biological studies where trace-level cadmium analysis is essential.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"40 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanostructure-Dependent Signal Intensity in Through-Hole Porous Alumina Membranes for Mass Spectrometry Imaging","authors":"Masahiro Kotani,&nbsp;Takashi Yanagishita","doi":"10.1002/rcm.10149","DOIUrl":"https://doi.org/10.1002/rcm.10149","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>Matrix-assisted laser desorption/ionization (MALDI) is a widely used analytical technique for measuring high-molecular-weight compounds such as proteins. However, in the low-molecular-weight region, interference peaks derived from the matrix occur. Surface-assisted laser desorption/ionization (SALDI), which is matrix-free, does not generate background noise in the low-molecular-weight region and has the advantages of simple sample preparation and reproducibility. We previously developed an ionization method using an anodic porous alumina membrane (APAM) as a SALDI substrate. In this study, we examined the effects of the surface nanostructural properties of APAMs, such as hole diameter and pitch, on the signal intensity in mass spectrometry (MS) imaging.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>APAMs were fabricated using electrolytes of oxalic, malonic, and malic acids and were evaluated by MS using droplet samples and MS imaging. Droplet samples were applied to the back surface of the APAMs. MS imaging was conducted using 20-μm-thick mouse brain sections to compare the signal-to-noise ratio (SNR) of each APAM.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>APAMs were fabricated with hole diameters (D_h) of 24–419 nm, interhole distances (D_int) of 100–625 nm, and open area ratios (OAR) of 5%–46%. From MS and MS imaging results, signal intensity at the same OAR increased in the order of D_int = 100, 625, and 270 nm, and the condition D_h/D_int = 131/270 nm provided the highest SNR. In addition, APAMs with a D_h of less than 84 nm and an OAR lower than 10% exhibited lower signal intensities.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>We fabricated APAMs under various conditions and identified the processing conditions that provided the highest SNR of SALDI imaging. SALDI imaging using the APAMs fabricated under these high SNR conditions is expected to be applicable in various fields such as materials science and metabolomics, as it does not generate interference peaks in the low-molecular-weight region.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"40 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extreme Rainfall Contribution and Isotopic Excursion of Tropical Storm Alberto in Northeastern Mexico","authors":"Odalys J. Ibarra-Alejos,&nbsp;Ricardo Sánchez-Murillo,&nbsp;Rene Ventura-Houle,&nbsp;Oscar Guevara Mansilla,&nbsp;Tomas Peña-Alonso,&nbsp;Bárbara A. Macías-Hernández,&nbsp;Luis González-Hita,&nbsp;Miguel A. Mejía-González","doi":"10.1002/rcm.10146","DOIUrl":"https://doi.org/10.1002/rcm.10146","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>Tropical cyclones are critical extreme rainfall events that can temporarily mitigate drought impacts in semiarid regions. Despite the well-known North Atlantic hurricane season, the propagation of isotopically distinct rainfall pulses across semiarid landscapes of Mesoamerica is largely unknown.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>In June 2024, tropical storm (TS) Alberto made landfall in northeastern Mexico. This study evaluates TS Alberto's monthly and annual rainfall contribution across Tamaulipas and Nuevo León and the isotopic excursions (δ¹⁸O and <i>d</i>-excess) in precipitation and surface water. Rainfall data from 86 stations and high-frequency isotope samples from Ciudad Victoria, Tampico, Chetumal, and the San Marcos River were analyzed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Results show that TS Alberto produced unprecedented rainfall, ranging from 0.22 to 11.41 times the 10-year June average, and up to 123% of the annual average in orographically influenced regions (i.e., variation is based on 86 locations across the region). Reservoir data highlight a rapid hydrological recovery, with dam levels in Tamaulipas and Nuevo León increasing significantly within weeks. Rainfall isotopic composition exhibited a broad spectrum (δ¹⁸O: −15.82‰ to −2.80‰), reflecting varying moisture sources, convective activity, and orographic effects. TS Alberto reproduced nearly the entire annual δ¹⁸O variability within a few hours, underscoring the strong isotopic imprint of extreme events. During TS Alberto's landfall and passage, an inverse trend between δ¹⁸O and precipitation amount was observed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The storm's isotopic signature was traced into surface waters, providing evidence of rapid river response and potentially for aquifer recharge. These findings emphasize the dual hydrological and isotopic role of TCs in modulating water scarcity and improving hydroclimate reconstructions in drought-prone landscapes.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"40 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/rcm.10146","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping the Metabolic Fate of Suzetrigine in Equine and Camel Models: A Step Toward Reliable Antidoping Detection","authors":"Shino Ann Koshy,&nbsp;Meleparappil Muhammed Ajeebsanu,&nbsp;Abdul Khader Karakka Kal,&nbsp;Michael Benedict Subhahar,&nbsp;Tajudheen K. Karatt,&nbsp;Moses Philip","doi":"10.1002/rcm.10147","DOIUrl":"https://doi.org/10.1002/rcm.10147","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>Effective pain management remains a persistent challenge, with opioids limited by tolerance, dependence, respiratory depression, and misuse. This has created demand for safer, nonaddictive alternatives. Suzetrigine (VX-548, Journavx) is a novel analgesic with promising efficacy, but its potential misuse in competitive racing requires investigation. Limited information is available on its metabolism and detection, emphasizing the need for metabolic characterization to aid antidoping strategies.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>In vitro metabolism of suzetrigine was evaluated using equine liver microsomes and homogenized camel liver. Metabolic profiling was carried out using high-resolution LC–HRMS. Fragmentation analysis was performed to assign metabolite structures and characterize biotransformation pathways.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Seven phase I metabolites (M1–M7) were identified, primarily via hydroxylation, methylation, demethylation, detrifluoromethylation, and cleavage of the pyridine-2-carboxamide moiety. One phase II metabolite (M8), formed by sulfation of the demethylated metabolite M5, was detected exclusively with camel liver. Metabolite structures were tentatively assigned based on mass fragmentation data, which showed characteristic neutral losses and diagnostic ions. Comparative analysis revealed that both species shared common phase I pathways, but camel liver demonstrated additional conjugation capacity.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study provides the first comprehensive characterization of suzetrigine metabolism in equine and camel liver systems. The results underscore metabolic variations, propose a biotransformation pathway, and offer crucial insights to support the development of antidoping detection strategies.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"40 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic and Metabolomic Mechanism of Ibuprofen, Chondroitin Sulfate, and Vitamins With Minerals Tablets (21) for Kashin–Beck Disease Treatment","authors":"Jinfeng Wen,&nbsp;Xingxing Deng,&nbsp;Zhengjun Yang,&nbsp;Hui Niu,&nbsp;Qian Zhang,&nbsp;Yijun Zhao,&nbsp;Gaowa Naren,&nbsp;Huan Liu,&nbsp;Feng Zhang,&nbsp;Xiaodong Yang,&nbsp;Cuiyan Wu","doi":"10.1002/rcm.10130","DOIUrl":"10.1002/rcm.10130","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Kashin–Beck disease (KBD) is an osteoarthropathy with no specific drug treatment. This study seeks to uncover the molecular mechanisms of ibuprofen, chondroitin sulfate, and vitamins with minerals tablets (21) in treating KBD.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Integrated transcriptomic and metabolomic analyses were performed on peripheral blood samples collected from four KBD patients before and 1 month after treatment. Differential gene expression was assessed using the DESeq package, while differential metabolites were identified through univariate and multivariate statistical analyses.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>After treatment, 5671 differential genes (|log₂FC| ≥ 1, q &lt; 0.05) were identified, with 4954 upregulated and 717 downregulated. Enriched pathways included PI3K-Akt, MAPK, ECM-receptor interaction, Rap1, and Ras signaling. Untargeted metabolomics analyses revealed five differentially expressed metabolites (VIP ≥ 1, |log₂FC| &gt; 0.58, <i>p</i> &lt; 0.05): L-glutamine, 2-methylpropan-2-amine, epsilon-caprolactam, phosphocholine, and 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine. Metabolite enrichment analysis suggested alterations in amino acid and fatty acid metabolism. Integrated analysis revealed key pathways such as alanine, aspartate, and glutamate metabolism and arginine biosynthesis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The combination of ibuprofen, chondroitin sulfate, and vitamins with minerals tablets (21) may alleviate KBD through modulation of key genes (GDNF, BDNF, CCL2, VEGFA, and PPARG) and glutamine-related metabolic pathways.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"39 24","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145184385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}