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Effect of extremely low frequency magnetic field in growth, CMCase, electric conductivity and DNA of Aspergillus niger 极低频磁场对黑曲霉生长、CMCase、电导率和DNA的影响
Pub Date : 2020-03-23 DOI: 10.21608/ejphysics.2020.29413.1042
Sahar E. Abo-Neima, M. El-Metwally
In the past years the extremely low frequency magnetic field (ELF-MF) of the millimeter range has been actively used in biology, medicine, biotechnology, in this study the impact of electromagnetic radiations in growth, carboxymethycellulase activity (CMCase), cell conductivity and RAPD DNA pattern of A. niger were investigated. Experimentally, when the fungus treated with magnetic field at constant intensity 50Hz, 10 mT at different exposure times for five days (2 h/day) the total biomass dry weight inhibited by 72.2% after the first 2 h. ELF-MF showed also inhibition effect of in CMCase activity of A. niger. The enzyme activity was decreased from 30 IU/ml to 22.5 IU/ml after only 2h of exposure and the treated fungus lost about 50% of its CMCase activity after 10 h.The treated fungus showed also decreased in conductivity of fungal cells from 1.78×10 -1 to 0.51×10 -5 Siemens/meter. The study extended to detect the changes in RAPD patterns of A. niger DNA before and after exposure to the 50 Hz frequency. In conclusion, ELF-MF can be used as a safe method in control the activity of higly resistant fungi as A. niger .
近年来,毫米范围的极低频磁场(ELF-MF)在生物学、医学、生物技术等领域得到了广泛的应用,本文研究了电磁辐射对黑曲霉生长、羧甲基纤维素酶活性(CMCase)、细胞电导率和RAPD DNA图谱的影响。实验结果表明,在恒定强度50Hz、10 mT、不同暴露次数的磁场条件下处理5 d (2 h/d),前2 h后总生物量干重下降72.2%,ELF-MF对黑僵菌的CMCase活性也有抑制作用。暴露2h后,酶活性从30 IU/ml下降到22.5 IU/ml, 10 h后,真菌的CMCase活性下降了约50%,真菌细胞的电导率也从1.78×10 -1下降到0.51×10 -5西门子/米。该研究扩展到检测暴露于50 Hz频率前后黑曲霉DNA RAPD模式的变化。综上所述,ELF-MF可以作为一种安全的方法来控制黑曲霉等高抗性真菌的活性。
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引用次数: 0
A 60 kWe Solar Organic Rankine Cycle (SORC) Power Generation inThailand 泰国60千瓦太阳能有机朗肯循环(SORC)发电项目
Pub Date : 2019-01-01 DOI: 10.22587/jasr.2019.15.4.2
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引用次数: 1
Assessment of High-Velocity Free Surface Flow Interaction with a Bridge Pier in A Curved Channel 弯曲河道中桥墩与高速自由表面流相互作用的评价
Pub Date : 2019-01-01 DOI: 10.22587/jasr.2019.15.1.1
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引用次数: 0
Phenotypic and Genomic in Organ Matching in Fetal Species 胎儿物种器官匹配的表型和基因组
Pub Date : 2019-01-01 DOI: 10.22587/jasr.2019.15.3.5
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引用次数: 0
Effect of Collaboration Through Social Media on Collaborative Overload, Burnout and Employee Engagement 社交媒体协作对协作超载、倦怠和员工敬业度的影响
Pub Date : 2019-01-01 DOI: 10.22587/jasr.2019.15.2.1
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引用次数: 0
Molecular Infection in Tumor Growth Through Hypoxic Disorder 低氧障碍在肿瘤生长中的分子感染
Pub Date : 2019-01-01 DOI: 10.22587/jasr.2019.15.4.4
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引用次数: 0
In Vitro Whitening Properties and Antioxidative Potentials of TheExtracts of Three Melastoma Leaves Species (M. malabathricum, M.hirta and M. decemfidum) 三种褐皮瘤叶提取物(M. malabathicum、M.hirta和M. decemfidum)的体外美白特性和抗氧化活性
Pub Date : 2019-01-01 DOI: 10.22587/jasr.2019.15.3.2
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引用次数: 0
Rainwater Harvesting In Varied Sizes Of Residential Plots In Dhaka City 达卡市不同大小住宅小区的雨水收集
Pub Date : 2019-01-01 DOI: 10.22587/jasr.2019.15.5.4
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引用次数: 0
Common Diabetogenic Agents Used in Experimental Animals: A Review 用于实验动物的常见糖尿病药物:综述
Pub Date : 2019-01-01 DOI: 10.22587/jasr.2019.15.5.5
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引用次数: 0
Visual Learning Tactics for Hearing Stimuli 听觉刺激的视觉学习策略
Pub Date : 2019-01-01 DOI: 10.22587/jasr.2019.15.2.5
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引用次数: 0
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