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Identification, 3D modelling, and expression analysis of protopanaxadiol and protopanaxatriol synthases in Vietnamese ginseng under vanadium elicitation 钒激发下越南人参原人参二醇和原人参三醇合成酶的鉴定、三维建模及表达分析
Pub Date : 2023-06-21 DOI: 10.1080/14620316.2023.2226656
Nguyen Quang Duc Tien, Hoang Kha, Tran Linh Anh, L. Man, Duong Tan Nhut, Nguyen Hoang Loc
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引用次数: 0
Fruit set is moderately dependent on insect pollinators in strawberry and is limited by the availability of pollen under natural open conditions 草莓的坐果对昆虫传粉者有一定的依赖性,在自然开放条件下受花粉有效性的限制
Pub Date : 2023-06-14 DOI: 10.1080/14620316.2023.2212670
C. Menzel
ABSTRACT Modern strawberry (Fragaria ×ananassa Duch.) cultivars are hermaphrodite and have fertile flowers, with the anthers releasing viable pollen. Cultivars are self-compatible and do not require cross-pollination. Studies supporting managed or wild insects are based on a few reports and there are problems with the methods used to assess pollination. This review examined the role of pollination in strawberry. The mean (± s.d. or standard deviation) pollinator dependence (PD) for yield (self-pollination versus open- or insect-assisted pollination) was 0.36 ± 0.26 (P < 0.001, N = 52 studies). The yields of plants exposed to supplementary insects were higher than those exposed to pollinators under natural open conditions, with a calculated pollen limitation (PL) of 0.20 ± 0.17 (P < 0.001, N = 20 studies). Fields close to semi-natural habitats, wildflowers, grass or hedges can have more pollinators and a greater diversity of pollinators than fields further away. However, a greater abundance of pollinators does not always lead to higher fruit set. Yield is dependent on insect pollinators (moderate pollinator dependence) and is limited by the availability of pollen under natural open conditions (moderate pollen limitation).
摘要:现代草莓(Fragaria ×ananassa Duch.)品种是雌雄同体,具有可育的花朵,花药释放有活力的花粉。品种是自交的,不需要异花授粉。支持管理昆虫或野生昆虫的研究基于少数报告,并且用于评估授粉的方法存在问题。本文综述了授粉在草莓中的作用。传粉者对产量(自花授粉与开放授粉或昆虫辅助授粉)的平均(±sd或标准差)依赖性为0.36±0.26 (P < 0.001, N = 52)。在自然开放条件下,辅助昆虫处理的植物产量高于传粉者处理,花粉限制(PL)为0.20±0.17 (P < 0.001, N = 20)。靠近半自然栖息地、野花、草或树篱的田地比远离的田地有更多的传粉者和更大的传粉者多样性。然而,更多的传粉者并不总是导致更高的坐果。产量依赖于昆虫传粉者(中度传粉者依赖),并受到自然开放条件下花粉可用性的限制(中度花粉限制)。
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引用次数: 1
Ascorbate-glutathione cycle and thioredoxin system are involved in nitric oxide alleviating excess nitrate stress in tomato seedlings 抗坏血酸-谷胱甘肽循环和硫氧还蛋白系统参与了一氧化氮缓解番茄幼苗过量硝酸盐胁迫的作用
Pub Date : 2023-05-31 DOI: 10.1080/14620316.2023.2218381
Xue Gan, Shengtai Qiao, Yuxuan Liang, Kunzhi Li, Huini Xu
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引用次数: 0
Physicochemical properties of peach fruit assessed by non-destructive methodology related to the development of Monilinia fructicola during postharvest 用无损方法评价桃果采后果实的理化性质及其与果霉病发生的关系
Pub Date : 2023-05-25 DOI: 10.1080/14620316.2023.2216227
L. C. Rozwalka, H. Thomazi‐Kleina, Walmes Marques Zeviani, Débora Leitzke Betemps, L. L. May-De-Mio
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引用次数: 0
Acclimatisation of orchids using plant growth-promoting bacteria and humic acids 利用植物促生长细菌和腐植酸驯化兰花
Pub Date : 2023-05-16 DOI: 10.1080/14620316.2023.2209569
Klever Cristiano Silveira, Raphael Oliveira de Melo, Marihus Altoé Baldotto, Lílian Estrela Borges Baldotto
ABSTRACT Acclimatisation of orchid seedlings propagated in vitro is a slow process that can be accelerated with the use of plant growth-promoting microorganisms and humic substances. The aim of this study was to evaluate the plant’s response to inoculation with plant growth-promoting bacteria and humic acids, as well as to their combined use, during the period of acclimatisation of Cymbidium sp. orchids. The treatments were control; application of humic acids; inoculation with Stenotrophomonas maltophilia; inoculation with Burkholderia cepacia; inoculation with S. maltophilia and B. cepacia; inoculation with S. maltophilia combined with application of humic acids; inoculation with B. cepacia combined with application of humic acids; inoculation with S. maltophilia and B. cepacia combined with application of humic acids. A total of 150 days after acclimatisation, the plants were biometrically and nutritionally evaluated. Inoculation with the bacterial strains separately promoted seedlings with greater N content; however, only inoculation with S. maltophilia, resulted in plants with more total dry matter in relation to the control. There was no response from application of humic acids in an isolated manner or in combination with the bacterial strains. The results indicate the biotechnological potential of the bacteria S. maltophilia in promoting the growth of the Cymbidium sp. orchid.
兰花苗的离体驯化是一个缓慢的过程,可以通过植物生长促进微生物和腐殖质物质的使用来加速。本研究的目的是评估植物对植物生长促进菌和腐植酸接种的反应,以及它们在驯化期间的组合使用。处理为对照;腐植酸的应用;接种嗜麦芽窄养单胞菌;接种洋葱伯克氏菌;接种嗜麦芽葡萄球菌和洋葱芽孢杆菌;接种嗜麦芽葡萄球菌配合施用腐植酸;接种洋葱芽孢杆菌配合施用腐植酸;接种嗜麦芽葡萄球菌和洋葱芽孢杆菌并施用腐植酸。驯化后共150天,对植物进行生物计量学和营养评价。分别接种菌株可促进幼苗的氮含量提高;而只接种嗜麦芽葡萄球菌,植株的总干物质含量高于对照。单独施用腐植酸或与菌株联合施用腐植酸均无反应。结果表明,嗜麦芽葡萄球菌具有促进兰花生长的生物技术潜力。
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引用次数: 0
Effects of preharvest bagging on soft nose disorder in mango (Mangifera indica L. cv. ‘Keitt’) during postharvest 采前套袋对芒果软鼻病的影响。“凯特”)在收获后
Pub Date : 2023-05-09 DOI: 10.1080/14620316.2023.2209083
Yuan Cheng, Ke Xu, Ying Wang, Yue Wang, Xiaolin Zheng
ABSTRACT Internal breakdown is a common physiological disorder in mango growth and development, and ‘Keitt’ mango is sensitive to soft nose disorder. In this study, the effect of preharvest bagging on the occurrence of soft nose disorder of ‘Keitt’ mango was studied. The results showed that the incidence rate of soft nose disorder in bagged mango fruit increased with the increase in the fruit size and storage time, and the contents of mainly mineral elements were also affected by fruit size. The bagging treatment decreased the contents of Ca, N, and K mineral elements and increased the contents of Fe, Cu, Mg, and P. Also, except for Ca, the contents of these elements in the decayed tissues of soft nose fruit were higher than those in the unaffected tissues. Using principal component and correlation coefficient analysis indicated that fruit size was related to the incidence of soft nose and mineral element imbalance rather than the lack of Ca being the main cause for internal breakdown. In conclusion, the preharvest bagging treatment affected mineral element content, and the fruit size of mango also affected the distribution and concentration of the mineral elements.
内部分解是芒果生长发育过程中常见的生理障碍,而“Keitt”芒果对软鼻障碍很敏感。本试验研究了采前套袋对‘Keitt’芒果软鼻病发生的影响。结果表明,袋装芒果软鼻病的发病率随着果实大小和贮藏时间的增加而增加,主要矿质元素的含量也受果实大小的影响。套袋处理使软鼻果腐烂组织中Ca、N、K矿质元素含量降低,Fe、Cu、Mg、p含量升高,除Ca外,其余矿质元素含量均高于未腐烂组织。主成分分析和相关系数分析表明,果实大小与软鼻病的发生和矿质元素失衡有关,而钙缺乏不是导致内部分解的主要原因。综上所述,采前套袋处理影响了矿质元素含量,果实大小也影响了矿质元素的分布和浓度。
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引用次数: 1
Characterization of guava (Psidium guajava L.) germplasm through morphological traits and SCoT markers 番石榴(Psidium guajava L.)种质资源的形态特征和SCoT标记鉴定
Pub Date : 2023-05-04 DOI: 10.1080/14620316.2022.2139767
Samriti Sharma, R. Kumari, H. Prasad, A. Walia, Raj Kumar, S. Dobhal
ABSTRACT Traditional breeding programmes have been limited to the selection and introduction of genotypes with promising agronomic characteristics, but studies focused on genetic diversity in the low hills of the North West(NW) Himalayas have not been conducted, which is very important for the identification of potential parents for breeding programmes in guava. In the present study, six best performing guava cultivars/hybrids were characterised using morphological descriptors and SCoT markers. Broad phenotypic variability among the guava cultivars/hybrids was detected using morphological descriptors. A set of 36 SCoT markers were used for polymorphism, out of which 31(86.1%) markers showed polymorphism, indicating high genetic variability in the guava cultivars/hybrids. During the analysis, 291 polymorphic amplicons were obtained, ranging from four to 19, with an average of 9.4 amplicons per primer and average polymorphic information content of 0.47.The UPGMA classified hybrids and cultivars into two groups. Based on morphological and molecular performance, L-49(Sardar) was ranked as the most promising cultivar for the low hills of NW Himalayan conditions. Morphological descriptors along with SCoT markers proved efficient in detecting the levels of genetic variability among the collections maintained in the field. These results can be used as an additional source of exploitation in guava breeding programmes.
传统的番石榴育种计划仅限于选择和引进具有良好农艺性状的基因型,而对西北喜马拉雅低山丘地区番石榴遗传多样性的研究尚未开展,这对番石榴育种计划的潜在亲本鉴定非常重要。本研究利用形态描述符和SCoT标记对6个表现最好的番石榴品种/杂交种进行了鉴定。利用形态描述符检测番石榴品种/杂交种间广泛的表型变异。利用36个SCoT标记进行多态性分析,其中31个(86.1%)标记表现多态性,表明番石榴品种/杂交种具有较高的遗传变异性。分析共获得291个多态性扩增子,从4个到19个不等,平均每个引物9.4个扩增子,平均多态性信息含量为0.47。UPGMA将杂交品种和栽培品种分为两类。基于形态和分子性能,L-49(Sardar)被评为西北喜马拉雅低山条件下最有前途的栽培品种。形态学描述符和SCoT标记被证明可以有效地检测田间保存的标本之间的遗传变异水平。这些结果可作为番石榴育种计划的额外开发来源。
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引用次数: 1
Comparison of fruit texture and aquaporin gene expression in papaya “Khak Nual” cultivated under varying conditions 不同栽培条件下番木瓜果实质地和水通道蛋白基因表达的比较
Pub Date : 2023-05-01 DOI: 10.1080/14620316.2023.2206396
P. Burns, P. Saengmanee, Winai Utkhao, A. Terdwongworakul, K. Thaipong, Uthaiwan Doung-ngern, Jingtar Siripanich
ABSTRACT The effects of cultivation conditions on the texture of unripe papaya fruit ‘Khak Nual’ were investigated. Fruit from papaya cultivated using raised bed conditions were crisper than those cultivated under open field conditions. Conversely, fruit cultivated under open field conditions were firmer. In all cases, mesocarp cells and intercellular spaces were larger in papaya fruit cultivated in raised beds, reflecting the higher relative humidity (RH) of the cultivation conditions and greater water availability. At harvest, TIP2–1, TIP4–1, SIP1 and PIP1–3 expression was significantly higher in fruit cultivated under raised bed conditions, which correlated with the larger cells of the mesocarp, the RH and the ready availability of water. There also appeared to be a link between aquaporin gene expression and crispness in papaya fruit from both conditions. Expression of TIP2–1 and TIP4–1 correlated with papaya fruit under open field conditions while SIP1 and PIP2–5 correlated with papaya fruit under raised bed conditions. The results clearly demonstrate the effect of cultivation conditions on fruit texture, with water availability being critical in the production of market-preferred crisp fruit with the optimal conditions. These conditions are reflected in the higher expression of the water transport aquaporins which likely control crispness.
摘要:研究了栽培条件对“Khak Nual”木瓜果实质地的影响。垄沟栽培的木瓜果实比露天栽培的果实更脆。相反,在露天条件下栽培的果实更结实。在所有情况下,凸起栽培的木瓜果实中果皮细胞和细胞间隙都较大,反映了栽培条件的相对湿度(RH)较高,水分有效性较高。收获时,在养床条件下培养的果实中,TIP2-1、TIP4-1、SIP1和PIP1-3的表达量显著升高,这与中果皮细胞体积较大、相对湿度和水分有效度有关。在两种情况下,水通道蛋白基因表达与木瓜果实的脆度之间似乎也存在联系。tip1 - 1和TIP4-1的表达与露天条件下的木瓜果实相关,而SIP1和PIP2-5的表达与垄沟条件下的木瓜果实相关。结果清楚地表明,栽培条件对果实质地的影响,水分有效性是在最佳条件下生产市场喜爱的脆果的关键。这些条件反映在可能控制脆度的水运水通道蛋白的高表达上。
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引用次数: 1
Propagation of cacao (Theobroma cacao L.) clone ‘CCN51’ using somatic embryogenesis: from pilot scale to commercial production 可可(Theobroma cacao L.)无性系‘CCN51’体细胞胚胎发生繁殖:从中试规模到商业化生产
Pub Date : 2023-04-27 DOI: 10.1080/14620316.2023.2205855
Ana María Henao Ramírez, Diana Maria Cano Martínez, Rodrigo Alberto Hoyos Sánchez, Aura Inés Urrea Trujillo
ABSTRACT The transition of promising technologies for the massive production of cacao trees from research to commercial scale is often difficult and expensive. As a result, the timeframe estimated for plantlet production is underestimated resulting in exceptionally long processes in the laboratory that make the production system unfeasible to bring to markets. Somatic embryogenesis (SE) is considered the most suitable and integrated technology for the large-scale production of clonal cocoa plants, compared to conventional methods. To date, practical application of SE to produce cocoa genotypes of interest has been limited, and for ‘drop-in’ technology replacements, price is of paramount importance for success. Therefore, the objective of this work was to produce the CCN51 genotype in a pilot scale in the laboratory with an approach to commercial production. The productive stages for a complex biological process such as SE and a reduction in production cycle time were defined. The production stages defined for SE were initiation, multiplication, maturation, germination, and acclimatization. The minimal time obtained for CCN51 production was 8.3 months: 30, 70, 50, 70, and 30 days, respectively. The decrease in the time of the production process directly influences the process cost and it represents a breakthrough in the technology development.
将有前景的可可树大规模生产技术从研究过渡到商业规模通常是困难和昂贵的。因此,估计的幼苗生产时间被低估,导致实验室过程异常漫长,使生产系统无法推向市场。与传统方法相比,体细胞胚胎发生(SE)被认为是最适合大规模生产克隆可可植株的综合技术。迄今为止,SE在生产感兴趣的可可基因型方面的实际应用有限,对于“插入式”技术替代品来说,价格是成功的最重要因素。因此,这项工作的目标是在实验室中以中试规模生产CCN51基因型,并逐步走向商业化生产。定义了SE等复杂生物过程的生产阶段和缩短生产周期。对SE的生产阶段定义为萌发、繁殖、成熟、发芽和驯化。生产CCN51所需的最短时间为8.3个月:分别为30、70、50、70和30天。生产过程时间的缩短直接影响到工艺成本,是技术发展的一大突破。
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引用次数: 0
Post-harvest conservation of mangosteen (Garcinia mangostana): Influence of fruit harvest stage and effectiveness of some practical conservation treatments 山竹的采后保存:果实采收期的影响及一些实际保存处理的效果
Pub Date : 2023-04-25 DOI: 10.1080/14620316.2023.2205409
Jean Florent Haba, Roland Kossonou N’guettia, Jean Kouao Koffi, Desirée Elichama Phaceli, Lassana Tioté, Adjé Franck Djalega, Ardjouma Dembélé
ABSTRACT Post-harvest losses caused by poor fruit storage can in some cases reach more than 80% of the crop. For the mangosteen crop, these losses are all the more important as they constitute a major problem. In Côte d’Ivoire, the few mangosteen preservation techniques have been developed mainly by traders and are based on limiting exchanges with the surrounding environment. However, these techniques are not very effective because they only preserve the fruit for a few days. In order to address this issue, the present study aims to evaluate the influence of the harvesting stage and the effectiveness of preservation treatments on the storage time and the evolution of several physical and biochemical parameters of the mangosteen. To this end, mangosteens harvested at two different stages of maturity were subjected to different preservation treatments (refrigeration, bagging, oil coating and blanching). The results showed that the storage time of the mangosteen was significantly longer with fruit harvested at the orange-red stage. Also, two of the four storage treatments evaluated, namely refrigeration and bagging, significantly extended the shelf life of the mangosteen (+15 and+6 days respectively with these treatments). In contrast to these treatments, blanching caused a reduction in fruit storage time.
在某些情况下,由于水果贮藏不良造成的收获后损失可达作物的80%以上。对于山竹作物来说,这些损失更为重要,因为它们构成了一个主要问题。在Côte科特迪瓦,少数山竹保存技术主要是由商人开发的,其基础是与周围环境的有限交流。然而,这些技术并不是很有效,因为它们只能保存水果几天。为了解决这一问题,本研究旨在评价采收阶段和保鲜处理效果对山竹果实贮藏时间和若干物理生化参数演变的影响。为此,在两个不同成熟阶段收获的山竹果进行了不同的保存处理(冷藏、装袋、涂油和焯水)。结果表明,在橙红色阶段收获的山竹果,贮藏时间明显延长。此外,在四种储藏处理中,冷藏和袋装两种处理显著延长了山竹果的保质期(分别为+15天和+6天)。与这些处理相比,焯水导致水果储存时间缩短。
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引用次数: 0
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The Journal of Horticultural Science and Biotechnology
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