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Review on crop load management in apple (Malus x domestica Borkh.) 苹果(Malus x domestica Borkh.)作物负荷管理综述
Pub Date : 2022-11-30 DOI: 10.1080/14620316.2022.2149425
P. Verma, Shivani Sharma, N. Sharma, N. Chauhan
ABSTRACT Apples grown often produce more fruit, but they are characterised by heavy bloom and fruit set, which results in undersized, poorly coloured, and low-quality apples. The optimal crop load, which is a quantitative measure, results in a steady annual yield and marketable fruit. Winter pruning technique, chemical thinning, hand thinning, mechanical thinning, and artificial spur extinction (ASE) are several crop load management techniques that all work together to improve return bloom and boost high-quality fruit production in apple, especially in high-density plantations. An overview of current studies to regulate apple crop load via apple-producing regions is provided in the article. In high-density apple orchards, this will help in determining the best crop load management strategies.
种植的苹果通常会结出更多的果实,但它们的特点是开花和坐果过重,这导致苹果体积小,颜色差,质量差。最佳作物负荷,这是一个定量的措施,结果是稳定的年产量和适销水果。冬季修剪技术、化学间伐、人工间伐、机械间伐和人工去节(ASE)是几种作物负荷管理技术,它们共同作用,提高苹果的回春开花和高质量果实产量,特别是在高密度种植园。本文综述了通过苹果产区调控苹果作物负荷的研究现状。在高密度苹果园中,这将有助于确定最佳的作物负荷管理策略。
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引用次数: 1
Aquaporin expression and changes in fatty acid levels in apple leaves subjected to partial root-zone irrigation 部分根区灌溉对苹果叶片水通道蛋白表达和脂肪酸水平的影响
Pub Date : 2022-11-30 DOI: 10.1080/14620316.2022.2147102
Hajar Ghafari, H. Hassanpour, M. Jafari, S. Besharat
ABSTRACT Partial root-zone drying (PRD) can induce changes in levels of the membrane fatty acids, transporter channels such as aquaporins (AQPs) and drought stress-responsive transcription factors (TFs) expression. The apple (Malus domestica (Suckow) Borkh. cv. Braeburn) trees were exposed to different PRD treatments for two consecutive years. The plasma-membrane intrinsic proteins (PIP1;1) and tonoplast-intrinsic proteins (TIP1;1) AQPs presented the highest expression in alternate partial root-zone drying (APRD75) and fixed partial root-zone drying (FPRD75) treatments than FPRD50 and control throughout the entire irrigation season, which was 120-140-fold higher in these treatments during some times. The TFs were highly expressed in APRD75 and FPRD75 treatments. The highest unsaturated fatty acid content was observed in APRD75 and FPRD75 treatments, and the lowest saturated fatty acid content was obtained in FPRD50 in both years. In addition to improving the water use efficiency (WUE) and maintaining the yield, APRD75 and FPRD75 treatments had a higher jasmonic acid (JA) content than the control. These results implied that changes in levels of the membrane fatty acids, AQPs, JA and TFs induced by APRD75 and FPRD75 treatments may be one of the possible ways for apple trees to adapt to the changing environmental conditions caused by PRD.
部分根区干燥(PRD)可引起膜脂肪酸水平、水通道蛋白(AQPs)等转运通道和干旱胁迫响应转录因子(TFs)表达的变化。苹果(家苹果)简历。Braeburn)树连续两年暴露于不同的PRD处理下。在整个灌溉季节,质膜内在蛋白(PIP1;1)和细胞质内在蛋白(TIP1;1)在交替根区干燥(APRD75)和固定根区干燥(FPRD75)处理中表达量均高于FPRD50和对照,在某些时期表达量高出120-140倍。TFs在APRD75和FPRD75处理中高表达。APRD75和FPRD75处理的不饱和脂肪酸含量最高,FPRD50处理的饱和脂肪酸含量最低。APRD75和FPRD75处理在提高水分利用效率和保持产量的同时,茉莉酸(JA)含量也高于对照。这些结果表明,APRD75和FPRD75处理诱导的膜脂肪酸、AQPs、JA和tf水平的变化可能是苹果树适应PRD引起的环境条件变化的途径之一。
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引用次数: 1
Relationships between the incidence of fire blight in apple orchards and plant nutritional imbalance indices 苹果果园火枯病发生与植株营养失衡指标的关系
Pub Date : 2022-11-27 DOI: 10.1080/14620316.2022.2147100
M. S. Tadayon, S. Sadeghi
ABSTRACT Fire blight disease is a major threat to the apple orchards in various regions of the world. The multivariate compositional nutrient diagnosis (CND) was considered to evaluate the effect of plant nutrient imbalance in development of fire blight in ‘golden delicious’ apple orchards in Fars province, Iran. To establish CND norms and the fire blight severity index, 81 commercial orchards were used. The CND reference norms (V*x) for a low fire blight severity subpopulation were calculated as V*N = 2.813 ± 0.186, V*P = 0.297 ± 0.147, V*K = 2.660 ± 0.149, V*Ca = 2.442 ± 0.218, V*Mg = 1.188 ± 0.061, V*S = 0.595 ± 0.399, V*Fe = −0.952 ± 0.212, V*Mn = −3.132 ± 0.195, V*Zn = −4.040 ± 0.366, V*Cu = −5.032 ± 0.177, V*B = −3.361 ± 0.120, V*Rd = 6.521 ± 0.128. According to the calculated leaf nutrient indices based on the CND reference norms and principal component analysis (PCA), leaf Ca, S, B and Zn deficiency and leaf K and P excess significantly correlated with the enhancement of fire blight severity index (FBSI). The reduction of global nutrient imbalance index (CND-r2) to below 20.38 decreases the severity of fire blight in ‘golden delicious’ apple orchards. Key policy highlights The CND reference norms for a low fire blight severity subpopulation was calculated. CND nutrient index significantly correlated with fire blight severity index. The reduction of global nutrient imbalance index decreases the severity of fire blight.
摘要:火疫病是世界上许多地区苹果果园的主要威胁。采用多元营养成分诊断(CND)评价了植物营养失衡对伊朗法尔斯省“金香”苹果园火疫病发展的影响。以81个商品果园为研究对象,建立了CND标准和疫病严重程度指数。CND参考规范(V * x)低火疫病严重程度分组人口被计算成V * N = 2.813±0.186,V * P = 0.297±0.147 V * K = 2.660±0.149,2.442±0.218 V * Ca =, = 1.188±0.061 V *毫克,V * S = 0.595±0.399 V *菲=−0.952±0.212,3.132±0.195 V * Mn =−,V *锌=−4.040±0.366 V *铜=−5.032±0.177 V * B =−3.361±0.120 V * Rd = 6.521±0.128。基于CND参考规范和主成分分析(PCA)计算的叶片养分指数表明,叶片Ca、S、B、Zn缺乏症和K、P过剩与火疫病严重指数(FBSI)的提高呈显著相关。全球营养失衡指数(cd -r2)降至20.38以下,“金香”苹果园的火枯病严重程度降低。计算了低火疫病严重程度亚群的CND参考规范。CND养分指数与疫病严重程度指数呈极显著相关。全球营养失衡指数的降低降低了火疫病的严重程度。
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引用次数: 0
Bulb development in garlic – a review 大蒜鳞茎发育研究进展
Pub Date : 2022-11-25 DOI: 10.1080/14620316.2022.2150326
K. M. Khokhar
ABSTRACT Many factors including cold conditioning, temperature, photoperiod, plant population, clove weight, soil nutrients, irrigation, plant growth regulators, and genetic responses influence bulb development in garlic. Garlic produces poor bulbs in warm and short-day conditions but cool and long-days induce flowering. Bulbs exposed before planting from 0° to 10°C for 8 weeks accelerate growth as the low temperatures modify the hormonal balance. Large cloves (>2–4 g) yield heavier bulbs than small (1–2 g) cloves. Plant population has an impact on bulb size: the higher the plant population, the smaller the bulb size. Garlic is sensitive to moisture stress especially during bulb initiation and development. Depending upon cultivar, soil type, and fertility status, NPK fertilisation for enhancement of bulb yield varies from 60–200, 20–75, and 40–166 kg ha−1, respectively. Low-temperature pre-treatment (4°C) increases salicylic acid (SA) concentration in the leaf sheath and enhances bulbing. Injecting garlic plants with gibberellin (GA3) solution increases clove number per bulb. Like onion, flowering and bulb formation in garlic are controlled by different (Flowering Locus T) FT genes. Two antagonistic FT-like genes regulate bulb formation. AsFT1 enhances bulb formation, while AsFT4 prevents AsFT1 up-regulation and inhibits bulbing.
冷调节、温度、光周期、植物种群、丁香质量、土壤养分、灌溉、植物生长调节剂和遗传响应等因素影响大蒜球茎发育。在温暖和日照短的条件下,大蒜产生的球茎很差,但凉爽和日照长则会开花。种植前将球茎暴露在0°到10°C的环境中8周,由于低温改变了激素平衡,球茎会加速生长。大瓣(> 2-4克)的球茎比小瓣(1-2克)的球茎重。植物种群对球茎大小有影响:植物种群越高,球茎大小越小。大蒜对水分胁迫非常敏感,特别是在鳞茎形成和发育过程中。根据品种、土壤类型和肥力状况的不同,氮磷钾施肥提高鳞茎产量的范围分别为60-200、20-75和40-166 kg ha - 1。低温预处理(4℃)增加叶鞘中水杨酸(SA)浓度,促进成球。向大蒜植株注射赤霉素(GA3)溶液可增加每个球茎的丁香数。与洋葱一样,大蒜的开花和鳞茎形成受不同的(开花位点T) FT基因控制。两个拮抗的ft样基因调控球茎的形成。AsFT1促进鳞茎形成,而AsFT4阻止AsFT1上调并抑制鳞茎形成。
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引用次数: 2
On the pros and cons of red photons for greenhouse tomato production: increasing the percentage of red photons improves LED efficacy but plant responses are cultivar-specific 红光子在温室番茄生产中的利弊:增加红光子的百分比可以提高LED的效率,但植物的反应是品种特异性的
Pub Date : 2022-11-21 DOI: 10.1080/14620316.2022.2147101
Paul Kusuma, Theoharis Ouzounis, David Hawley, T. Kerstens, L. Marcelis, E. Heuvelink
ABSTRACT Supplemental lighting is necessary for winter greenhouse tomato production, and optimised spectra must consider both yield and energy use. In a 20-week winter greenhouse experiment, two common commercial tomato cultivars – ‘Merlice’ and ‘Brioso’ – were grown under four LED spectra with 38%, 63%, 81%, and 95% red photons. As the percentage of red photons increased, the blue and green percentages decreased (not at the same ratio). Stem length, specific leaf area, and dry mass partitioning were not significantly affected by spectra in either cultivar, but increasing the red percentage from 38% to 95% decreased harvested fruit fresh mass by 13%, total plant dry mass by 7.1%, and fruit dry mass by 9.5% in ‘Merlice’. There were no significant differences in these parameters for ‘Brioso’. The yield kWh−1 increased with increasing percent red in both cultivars because LED fixtures with higher fractions of photons from 660 nm red LEDs have higher photon efficacies (µmol J−1). The efficacies of the lamps in this study were estimated to range from 2.6 to 3.6 µmol J−1. Growers must consider tradeoffs that can occur between yield and efficacy in some cultivars, and how these factors apply to their situation, when choosing the spectrum for their greenhouse.
冬季温室番茄生产需要补充照明,优化光谱必须同时考虑产量和能源使用。在一项为期20周的冬季温室试验中,两种常见的商业番茄品种“Merlice”和“Brioso”在四种LED光谱下生长,红色光子分别为38%、63%、81%和95%。随着红色光子百分比的增加,蓝色和绿色百分比减少(不是以相同的比例)。茎长、比叶面积和干质量分配均不受光谱的显著影响,但赤色率从38%提高到95%,收获果实鲜质量下降13%,植株总干质量下降7.1%,果实干质量下降9.5%。“Brioso”在这些参数上没有显著差异。在两个品种中,产量kWh−1随红色百分比的增加而增加,因为具有更高比例的660 nm红色LED的LED灯具具有更高的光子效率(µmol J−1)。在这项研究中,灯的效率估计在2.6到3.6µmol J−1之间。在为温室选择光谱时,种植者必须考虑在某些品种的产量和功效之间可能发生的权衡,以及这些因素如何适用于他们的情况。
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引用次数: 1
Extension of postharvest life by application of edible coatings on tomatoes var. 234 with silencing of the TomLoxb gene 沉默TomLoxb基因的番茄品种234应用可食用涂层延长采后寿命
Pub Date : 2022-11-15 DOI: 10.1080/14620316.2022.2142678
Wendy Marisol Mazón-Abarca, Elizabeth León-García, J. A. Ramírez De León, Javier De La Cruz-Medina, H. García
ABSTRACT Fruit mutants with the silenced TomloxB gene (tomato lipoxygenase B) have proven to be an alternative in extending postharvest life; however, their interaction with other classical technologies, such as the use of edible coatings, has been scarcely explored. The synergy between the two treatments can improve aspects of fruit quality during its postharvest life or even increase this period. Therefore, the objective of this study was to evaluate the effect of coatings (candelilla wax (CW) and Semperfresh® (S)) on the postharvest life and lipoxygenase activity of genetically modified (GM) tomatoes. Wild Type (WT) and GM tomatoes with the antisense TomLoxB gene insertion were used. LOX activity values on day 18 were 721 U/mg protein, in fruits coated with CW, below the WT fruit (859 U/mg protein) and other GM fruits with other coatings (916 to 996 U/mg protein). CW was the coating that exhibited the best results in preserving the postharvest life of the GM tomato for up to 30 days, compared to the other coatings (24 days). Genetic modification in tomatoes prolonged their postharvest life, which increased further with the use of coatings such as CW.
具有沉默TomloxB基因(番茄脂氧合酶B)的果实突变体已被证明是延长采后寿命的一种选择;然而,它们与其他经典技术的相互作用,如食用涂层的使用,很少被探索。两种处理之间的协同作用可以改善果实采后生命期的品质,甚至可以延长果实采后生命期。因此,本研究的目的是评估涂层(candelilla wax, CW)和Semperfresh®(S))对转基因番茄采后寿命和脂氧合酶活性的影响。以野生型(WT)和插入反义TomLoxB基因的转基因番茄为研究对象。第18天的LOX活性值为721 U/mg蛋白,在CW包覆的果实中,低于WT果实(859 U/mg蛋白)和其他包覆的转基因果实(916 ~ 996 U/mg蛋白)。与其他涂层(24天)相比,CW涂层在保存转基因番茄采后寿命方面表现出最佳效果,最长可达30天。番茄的基因改造延长了它们的采后寿命,使用CW等涂层后寿命进一步延长。
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引用次数: 0
Vertical farming - smart urban agriculture for enhancing resilience and sustainability in food security 垂直农业——提高粮食安全韧性和可持续性的智慧城市农业
Pub Date : 2022-11-12 DOI: 10.1080/14620316.2022.2141666
Soojin Oh, Chungui Lu
ABSTRACT Global food security has been significantly threatened by the Covid-19 pandemic and several prolonged challenges such as climate change, population increases, shortage of natural resources, energy crisis, and rapid urbanisation worldwide. Although numerous attempts have been made to secure resilience in the food system, many countries are suffering from hunger and malnutrition, particularly in African and some Asian countries. This review paper presents one of the sustainable farming practices – vertical farming that could play a key role in mitigating global food security in the current uncertain world. It addresses the recent development of vertical farming with advanced precision monitoring and controlling system by the Internet of Things (IoT) applications. It also provides information about the opportunities and challenges of vertical-urban agriculture and how urban agriculture meets economic, social and educational needs.
2019冠状病毒病大流行以及气候变化、人口增长、自然资源短缺、能源危机和全球快速城市化等一系列长期挑战严重威胁着全球粮食安全。尽管为确保粮食系统的复原力作出了许多努力,但许多国家仍在遭受饥饿和营养不良,特别是在非洲和一些亚洲国家。这篇综述文章介绍了可持续农业实践之一——垂直农业,在当前不确定的世界中,垂直农业可能在缓解全球粮食安全方面发挥关键作用。它通过物联网(IoT)应用解决了垂直农业的最新发展,并提供了先进的精确监控系统。它还提供了关于垂直城市农业的机遇和挑战以及城市农业如何满足经济、社会和教育需求的信息。
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引用次数: 8
Hormone profiling in artificially induced ‘crumbly’ fruit in raspberry (Rubus idaeus L.) at two different development stages 两个不同发育阶段人工诱导树莓“易碎”果实的激素谱分析
Pub Date : 2022-11-03 DOI: 10.1080/14620316.2022.2141140
Luca M. Scolari, R. D. Hancock, J. Graham, Sabine Freitag, S. Verrall, Amanda R. Moreno-Mellado, J. W. Allwood
ABSTRACT In red raspberry, a condition known as ‘crumbly’ fruit causes the formation of misshapen fruits with drupelets reduced in number but enlarged in size. Complex fruits such as those of raspberry, formed by the aggregation of many fertilised ovaries, require a regulating mechanism to coordinate and synchronise their growth and development. The receptacle was hypothesised as the leading hub that, by means of hormonal crosstalk with the fertilised ovaries, regulates the fruit growth process. Experiments were designed to perturb the regulating system by damaging the receptacle of hand pollinated flowers of the floricane-fruiting variety Glen Ample to produce artificially crumbly fruits. Hormone profiling was performed in control and artificially induced crumbly fruit samples at two stages of development green developing fruit and red ripe fruit. Of the hormones that could be adequately detected and quantified, abscisic acid (ABA) was elevated in the receptacle of crumbly samples at both developmental stages while SA was higher only in green fruit receptacles. In the druplets, trans-zeatin (tZ) concentration was greater in green than red fruit but the crumbly treatment did not influence content. The data highlight a potential role for these three phytohormones in the development of misshapen ‘crumbly-like’ fruits.
在红覆盆子中,一种被称为“易碎”果实的情况导致形成畸形的果实,小核果数量减少,但尺寸增大。复杂的果实,如覆盆子,由许多受精卵聚集而成,需要一个调节机制来协调和同步它们的生长和发育。花托被假设为主要的枢纽,通过与受精卵的激素串扰,调节果实的生长过程。实验设计通过破坏florican果实品种Glen Ample的手授粉花的花托来干扰调节系统,以产生人工易碎的果实。在青熟果和红熟果两个发育阶段对对照和人工诱导的易碎果样品进行激素分析。在可充分检测和定量的激素中,脆性样品在两个发育阶段的花托中脱落酸(ABA)均升高,而SA仅在绿果花托中升高。在小粒中,绿果的反式玉米素(tZ)浓度高于红果,但碎化处理对其含量没有影响。这些数据强调了这三种植物激素在畸形“易碎”水果发育中的潜在作用。
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引用次数: 0
A review of productivity in strawberry: do the plants need larger canopies, more flowers, or higher CO2 assimilation for higher yields? 对草莓生产力的回顾:为了更高的产量,植物是否需要更大的冠层,更多的花,或者更高的二氧化碳吸收?
Pub Date : 2022-11-02 DOI: 10.1080/14620316.2022.2077240
Christopher Michael Menzel
ABSTRACT Productivity in strawberry (Fragaria ×ananassa Duch.) is dependent on the production of new crowns, leaves and inflorescences, with fruit growth dependent on CO2 assimilation in the leaves. The yields of new cultivars peaked in the 1970s and 1980s in many locations, possibly because of the focus of breeding on fruit quality and other plant traits and the loss of diversity across commercial breeding populations. It is not known if higher yields are related to higher plant growth or higher CO2 assimilation in the leaves. This review assessed whether cultivars need larger canopies, more flowers, or higher CO2 assimilation for higher yields. There were moderate to strong relationships between plant growth or yield, and net CO2 assimilation per leaf area (P < 0.05; R 2 = 0.42 to 0.84) or the stability of net CO2 assimilation (P = 0.052, R 2 = 0.48) across cultivars and environments. Carbon dioxide enrichment increased mean (± SE) net CO2 assimilation by 88 ± 40% compared with plants at ambient and increased yield by 112 ± 73%. There was a linear decrease in yield as light levels decreased below full sun (P < 0.001, R 2 = 0.66), and linear decreases in yield the longer two cultivars were grown under 20% full sun (P = 0.002, R 2 = 0.84; P < 0.001, R 2 = 0.86). Daily gross primary productivity (GPP, mol CO2 per m2) estimated by measuring the changes in the concentration of CO2 above a crop was still increasing when the PAR (photosynthetic active radiation) was above 2,000 µmol per m2 per s (rectangular hyperbola models, R 2s = 0.44 or 0.72). There were linear decreases in yield as the number of leaves (P < 0.05, R 2 = 0.70 to 0.90), crowns (P < 0.001, R 2 = 0.82) or flowers (P < 0.05, R 2 = 0.51) were removed from plants. There were moderate to strong phenotypic correlations (R P > 0.50, N = 47 studies) and weak correlations (R P < 0.50, N = 69 studies) between yield and vegetative growth and flowering. Estimate of broad- and narrow-sense heritability for canopy growth and yield are low to moderate (H 2 and h 2 = 0.15 to 0.40). Cultivars produce high yields in different ways, suggesting that breeders consider the relationship between yield and plant architecture when developing cultivars. Initial experiments should include data on the number of leaves, crowns and inflorescences per plant. Subsequent experiments should include data on leaf area index (LAI) and/or LAD (leaf area density) using image analysis technology. More research is required to determine the value of measuring net CO2 assimilation per leaf area, with current technology time-consuming and the results variable. The use of image analyses to characterise the canopy will assist the development of high-yielding cultivars. Genome-wide association (GWA) and genomic prediction (GP) will also accelerate the identification of high-yielding populations and individuals.
草莓(Fragaria ×ananassa Duch.)的生产力依赖于新冠、叶片和花序的产生,果实的生长依赖于叶片中的二氧化碳同化。在许多地方,新品种的产量在20世纪70年代和80年代达到顶峰,可能是因为育种的重点是果实质量和其他植物性状,以及商业育种群体多样性的丧失。目前尚不清楚较高的产量是否与较高的植物生长或叶片中较高的二氧化碳同化有关。这篇综述评估了品种是否需要更大的冠层、更多的花或更高的二氧化碳同化来获得更高的产量。植物生长或产量与每叶面积净CO2同化呈中~强相关(P < 0.05;r2 = 0.42 ~ 0.84)或不同品种和环境间CO2净同化的稳定性(P = 0.052, r2 = 0.48)。与环境下的植物相比,二氧化碳富集使平均(±SE)净CO2同化提高88±40%,产量提高112±73%。在全日照条件下,随着光照水平的降低,产量呈线性下降(P < 0.001, r2 = 0.66);在20%全日照条件下,两个品种生长时间越长,产量呈线性下降(P = 0.002, r2 = 0.84;P < 0.001, r2 = 0.86)。当PAR(光合有效辐射)高于2000µmol / m2 / s时,通过测量作物上方CO2浓度变化估算的日总初级生产力(GPP, mol CO2 / m2)仍在增加(矩形双曲线模型,r2 = 0.44或0.72)。除叶数(P < 0.05, r2 = 0.70 ~ 0.90)、冠数(P < 0.001, r2 = 0.82)和花数(P < 0.05, r2 = 0.51)均呈线性下降。产量与营养生长和开花存在中强相关(R P > 0.50, N = 47)和弱相关(R P < 0.50, N = 69)。冠层生长和产量的广义和狭义遗传力估计值为低至中等(h2和h2 = 0.15至0.40)。品种以不同的方式获得高产,这表明育种者在培育品种时考虑了产量与植株结构之间的关系。最初的实验应包括每株植物的叶子、冠和花序的数量。后续实验应包括叶面积指数(LAI)和/或LAD(叶面积密度)数据,使用图像分析技术。要确定测量每叶面积净CO2同化的价值,还需要进行更多的研究,目前的技术耗时且结果多变。利用图像分析来表征冠层将有助于高产品种的开发。全基因组关联(GWA)和基因组预测(GP)也将加速高产群体和高产个体的鉴定。
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引用次数: 0
Integrated full-length transcriptome and metabolome analysis of the early defense mechanisms in Paulownia witches’ broom disease 泡桐丛枝病早期防御机制的综合全长转录组和代谢组分析
Pub Date : 2022-10-27 DOI: 10.1080/14620316.2022.2136586
Xiaogai Zhao, Xibing Cao, Yabing Cao, Zhenli Zhao, Guoqiang Fan
ABSTRACT Paulownia witches’ broom (PaWB) is a devastating disease of Paulownia spp. caused by phytoplasma infection. To reveal the reason for the absence of morphological changes at the early stage of phytoplasma infection, in this study, a combination of second-and third-generation sequencing (single-molecule real-time long-read isoform) and liquid chromatography tandem mass spectrometry were performed to analyse the changes of full-length transcripts and metabolites at the early stage of phytoplasma infection. A total of 140,528 non-redundant full-length transcripts, 597 transcription factors, 1,658 long non-coding RNAs, and 645 metabolites were identified. The third-generation sequencing improved the annotation of the reference Paulownia fortunei (Seem.) Hemsl. genome by 8.86%. Combination analysis of the full-length transcriptome and metabolome revealed that phenolics and phenylalanine ammonia-lyase synthesis related genes were significantly up-regulated in the early stage of phytoplasma-infected P. fortunei. Based on the results of full-length transcriptome and metabolome integrative analysis, we constructed an early defence model diagram of PaWB disease. Our results provide powerful insights into the reason for absence of morphological at the early stage of phytoplasma infection.
泡桐丛枝病(Paulownia witches ' broom, PaWB)是泡桐属植物原体侵染引起的一种毁灭性病害。为了揭示植物原体感染早期形态变化缺失的原因,本研究采用第二代和第三代测序(单分子实时长读异构体)和液相色谱串联质谱相结合的方法,分析了植物原体感染早期全长转录本和代谢物的变化。共鉴定出140528个非冗余全长转录物、597个转录因子、1658个长链非编码rna和645个代谢物。第三代测序改进了文献泡桐(Paulownia fortunei, Seem.)的注释。Hemsl。基因组减少8.86%。全长度转录组和代谢组的组合分析显示,酚类物质和苯丙氨酸解氨酶合成相关基因在植物原体感染早期显著上调。基于全长转录组和代谢组整合分析的结果,我们构建了PaWB疾病的早期防御模型图。我们的结果为植物原体感染早期形态缺失的原因提供了强有力的见解。
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引用次数: 0
期刊
The Journal of Horticultural Science and Biotechnology
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