Pub Date : 2023-03-31DOI: 10.1080/14620316.2023.2195863
L. Arhin, S. Abdullah, Juju Nakasha Jaafar, Shairul Izan Ramlee
ABSTRACT Coconut (Cocos nuciferaL.) is a multipurpose plantation crop with versatile uses, including as a refreshing drink, edible oil, and, nutraceuticals. Its nutraceutical properties have increased demand in global markets. However, coconut productivity remains low owing to inadequate improved planting materials. This challenge has created a high demand and low supply gap in the coconut industry, which needs to be addressed through breeding methods that can increase productive cultivars in a shorter time. Breeding methods, such as mass selection and hybridization between dwarf and tall cultivars, are the main coconut improvement methods because of their simplicity and ease of use. The hybrids produced, however, take a long time (4-6 years) to bear fruits due to genes inherited from tall parents. Dwarf hybrids that bear fruits in a shorter time (3 years) are less exploited. Developing these coconut types through efficient breeding methods can fill the supply and demand gaps created in the coconut industry. Marker-assisted selection and multi-omics, have been used to identify quantitative traits of agronomic importance to increase genetic gains in dwarf coconut cultivars. This review article summarizes the advances in coconut improvement through conventional breeding, marker-assisted selection, and multi-omics approaches, focusing on dwarf coconut cultivars.
{"title":"Conventional and modern breeding technologies for improving dwarf coconut cultivars: A review","authors":"L. Arhin, S. Abdullah, Juju Nakasha Jaafar, Shairul Izan Ramlee","doi":"10.1080/14620316.2023.2195863","DOIUrl":"https://doi.org/10.1080/14620316.2023.2195863","url":null,"abstract":"ABSTRACT Coconut (Cocos nuciferaL.) is a multipurpose plantation crop with versatile uses, including as a refreshing drink, edible oil, and, nutraceuticals. Its nutraceutical properties have increased demand in global markets. However, coconut productivity remains low owing to inadequate improved planting materials. This challenge has created a high demand and low supply gap in the coconut industry, which needs to be addressed through breeding methods that can increase productive cultivars in a shorter time. Breeding methods, such as mass selection and hybridization between dwarf and tall cultivars, are the main coconut improvement methods because of their simplicity and ease of use. The hybrids produced, however, take a long time (4-6 years) to bear fruits due to genes inherited from tall parents. Dwarf hybrids that bear fruits in a shorter time (3 years) are less exploited. Developing these coconut types through efficient breeding methods can fill the supply and demand gaps created in the coconut industry. Marker-assisted selection and multi-omics, have been used to identify quantitative traits of agronomic importance to increase genetic gains in dwarf coconut cultivars. This review article summarizes the advances in coconut improvement through conventional breeding, marker-assisted selection, and multi-omics approaches, focusing on dwarf coconut cultivars.","PeriodicalId":22704,"journal":{"name":"The Journal of Horticultural Science and Biotechnology","volume":"119 1","pages":"551 - 562"},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76119165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-28DOI: 10.1080/14620316.2023.2192213
Yiqing Wang, Rong Ding, Kezhong Zhang, Jinteng Cui
ABSTRACT Rose (Rosa hybrida L.) is one of the major cut flowers in the world. Studying the molecular mechanism of auxin regulation in growth is of great significance for enhancing the understanding of the growth and development processes in rose and informing accurate exogenous auxin application in rose production. However, the response mechanism of rose to miRNA-mediated auxin signal transduction is unclear. In this study, rose plants were treated with IAA, and 75 known miRNAs and 168 novel miRNAs were identified by small RNA sequencing. Among them, 19 known miRNAs and 42 miRNAs were differentially expressed. Many differential miRNAs demonstrated staged responses to auxin treatment. The targeted relationship between miRNA and key transcription factors regulated by auxin in rose was analysed, and the target genes in the ARF family and AUX/IAA family were screened. By using quantitative real-time PCR(qRT-PCR) to verify the expression patterns of the miRNA regulating the auxin signal transduction pathway and its target gene, we found that miR156a, miR160a, miR164a, miR167d, miR396b-3p, novel_miR_189, novel_miR_74, novel_miR_8, and novel_miR_207 were anti-correlated with the ARF family, and miR390a-3p and novel_miR_101 were anti-correlated with the AUX/IAA family. These results provide a theoretical basis for further studies on the auxin regulatory mechanisms in rose.
{"title":"Analysis of the effect of exogenous auxin on the regulatory relationship between miRNAs and their target genes in rose using high-throughput sequencing","authors":"Yiqing Wang, Rong Ding, Kezhong Zhang, Jinteng Cui","doi":"10.1080/14620316.2023.2192213","DOIUrl":"https://doi.org/10.1080/14620316.2023.2192213","url":null,"abstract":"ABSTRACT Rose (Rosa hybrida L.) is one of the major cut flowers in the world. Studying the molecular mechanism of auxin regulation in growth is of great significance for enhancing the understanding of the growth and development processes in rose and informing accurate exogenous auxin application in rose production. However, the response mechanism of rose to miRNA-mediated auxin signal transduction is unclear. In this study, rose plants were treated with IAA, and 75 known miRNAs and 168 novel miRNAs were identified by small RNA sequencing. Among them, 19 known miRNAs and 42 miRNAs were differentially expressed. Many differential miRNAs demonstrated staged responses to auxin treatment. The targeted relationship between miRNA and key transcription factors regulated by auxin in rose was analysed, and the target genes in the ARF family and AUX/IAA family were screened. By using quantitative real-time PCR(qRT-PCR) to verify the expression patterns of the miRNA regulating the auxin signal transduction pathway and its target gene, we found that miR156a, miR160a, miR164a, miR167d, miR396b-3p, novel_miR_189, novel_miR_74, novel_miR_8, and novel_miR_207 were anti-correlated with the ARF family, and miR390a-3p and novel_miR_101 were anti-correlated with the AUX/IAA family. These results provide a theoretical basis for further studies on the auxin regulatory mechanisms in rose.","PeriodicalId":22704,"journal":{"name":"The Journal of Horticultural Science and Biotechnology","volume":"70 1","pages":"745 - 757"},"PeriodicalIF":0.0,"publicationDate":"2023-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83744931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-23DOI: 10.1080/14620316.2023.2192230
S. Hussain, Hamza Sohail, Iqra Noor, Shakeel Ahmad, S. Ejaz, M. Ali, S. Haider, Muhammad Sohail, Hayat Jaffer, S. Ercişli, R. Morillon, M. Khalid
ABSTRACT Citrus plants face various abiotic stresses like drought during their life span which significantly affect their active growth and development. Tetraploid (4×) plants are more adaptable to environmental constraints than diploid (2×) plants by inducing critical physiological and biochemical processes. In this study, tetraploid and their corresponding diploid sour orange rootstocks were subjected to drought stress for 12 days. Results revealed that drought stress significantly affected plant physiology by reducing photosynthesis rate, stomatal conductance, transpiration rate, and leaf colour, which was prominent in diploid plants compared to tetraploid plants. In diploid plants, phenotypic differences (i.e. wilting and rolling of leaves) were also significant. Diploids with more accumulation of malondialdehyde and hydrogen peroxide in their leaves and roots exhibited more oxidative damage. It was observed that tetraploid plants had higher activities of peroxidase and catalase enzymes, while lower superoxide dismutase was recorded in the leaves and roots of plants. Higher glycine betaine, proline, total soluble protein, total phenolic content, and antioxidant activities were also observed in the leaves and roots of tetraploids. Overall, results suggest that tetraploids in citrus can have better defence mechanisms that help them to sustain under water deficit scenarios.
{"title":"Physiological and biochemical determinants of drought tolerance in tetraploid vs diploid sour orange citrus rootstock","authors":"S. Hussain, Hamza Sohail, Iqra Noor, Shakeel Ahmad, S. Ejaz, M. Ali, S. Haider, Muhammad Sohail, Hayat Jaffer, S. Ercişli, R. Morillon, M. Khalid","doi":"10.1080/14620316.2023.2192230","DOIUrl":"https://doi.org/10.1080/14620316.2023.2192230","url":null,"abstract":"ABSTRACT Citrus plants face various abiotic stresses like drought during their life span which significantly affect their active growth and development. Tetraploid (4×) plants are more adaptable to environmental constraints than diploid (2×) plants by inducing critical physiological and biochemical processes. In this study, tetraploid and their corresponding diploid sour orange rootstocks were subjected to drought stress for 12 days. Results revealed that drought stress significantly affected plant physiology by reducing photosynthesis rate, stomatal conductance, transpiration rate, and leaf colour, which was prominent in diploid plants compared to tetraploid plants. In diploid plants, phenotypic differences (i.e. wilting and rolling of leaves) were also significant. Diploids with more accumulation of malondialdehyde and hydrogen peroxide in their leaves and roots exhibited more oxidative damage. It was observed that tetraploid plants had higher activities of peroxidase and catalase enzymes, while lower superoxide dismutase was recorded in the leaves and roots of plants. Higher glycine betaine, proline, total soluble protein, total phenolic content, and antioxidant activities were also observed in the leaves and roots of tetraploids. Overall, results suggest that tetraploids in citrus can have better defence mechanisms that help them to sustain under water deficit scenarios.","PeriodicalId":22704,"journal":{"name":"The Journal of Horticultural Science and Biotechnology","volume":"6 1","pages":"772 - 785"},"PeriodicalIF":0.0,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78775457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-21DOI: 10.1080/14620316.2023.2189167
Y. Zhong, De Wang, Zhiwei Deng, Minghui Fu
ABSTRACT Eichhornia crassipes (Mart.) Solms can absorb nitrogen and phosphorus efficiently from water. Cloning the phosphate transporter 1 from E. crassipes (EcPHT1) and studying its characteristics can help to explain the molecular mechanism of absorption and transportation of inorganic phosphate (Pi) in E. crassipes and develop new genetic resources for phosphate transport to aid genetic breeding of crops with increased phosphate use efficiency. In this paper, we have used RACE method to clone the EcPT1 with 1572 bps which encoded a polypeptide of 523 amino acids and contained 11 transmembrane regions which was the typical structure of plant PHT1. The phylogenetic analysis also showed EcPT1 was closely related to other plant PHT1s. Quantitative real-time RT-PCR (qRT-PCR) showed that the EcPT1 expression in roots was up-regulated under low phosphorus stress . Moreover the EcPT1 expression in leaves was suppressed after E. crassipes was transferred to sufficient Pi from deficient Pi. Yeast functional complementation tests showed that the EcPT1 could complement the phosphate transport function of the Pi deficient yeast PHO84 in 175 μM Pi and make it grow best in pH = 5 or 6. All these results indicated that EcPT1 might be a phosphate transporter gene. Its expression was different in different tissues.
{"title":"Cloning and Characterisation of a novel phosphate transporter gene EcPT1 from Eichhornia crassipes","authors":"Y. Zhong, De Wang, Zhiwei Deng, Minghui Fu","doi":"10.1080/14620316.2023.2189167","DOIUrl":"https://doi.org/10.1080/14620316.2023.2189167","url":null,"abstract":"ABSTRACT Eichhornia crassipes (Mart.) Solms can absorb nitrogen and phosphorus efficiently from water. Cloning the phosphate transporter 1 from E. crassipes (EcPHT1) and studying its characteristics can help to explain the molecular mechanism of absorption and transportation of inorganic phosphate (Pi) in E. crassipes and develop new genetic resources for phosphate transport to aid genetic breeding of crops with increased phosphate use efficiency. In this paper, we have used RACE method to clone the EcPT1 with 1572 bps which encoded a polypeptide of 523 amino acids and contained 11 transmembrane regions which was the typical structure of plant PHT1. The phylogenetic analysis also showed EcPT1 was closely related to other plant PHT1s. Quantitative real-time RT-PCR (qRT-PCR) showed that the EcPT1 expression in roots was up-regulated under low phosphorus stress . Moreover the EcPT1 expression in leaves was suppressed after E. crassipes was transferred to sufficient Pi from deficient Pi. Yeast functional complementation tests showed that the EcPT1 could complement the phosphate transport function of the Pi deficient yeast PHO84 in 175 μM Pi and make it grow best in pH = 5 or 6. All these results indicated that EcPT1 might be a phosphate transporter gene. Its expression was different in different tissues.","PeriodicalId":22704,"journal":{"name":"The Journal of Horticultural Science and Biotechnology","volume":"37 1","pages":"580 - 590"},"PeriodicalIF":0.0,"publicationDate":"2023-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85609481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-17DOI: 10.1080/14620316.2023.2185165
F. Ahmadpoor, N. Zare, Rasool Asghari, Parisa Sheikhzadeh-Mosadeg
ABSTRACT In the present study, we investigated the effect of plant growth regulators and explant type on callus induction, cell suspension culture growth, antioxidant enzyme activity, and accumulation of rutin, quercetin, and kaempferol in M. azedarach L. cell cultures. Our studies showed that the inflorescence and petiole explants had a high percentage of callus induction compared to the leaf explants, whereas the highest callus growth was obtained from the leaf explants cultured on the MS medium containing 5 mg l−1 NAA and 5 mg l−1 BAP and 5 mg l−1 NAA and 3 mg l−1 kinetin Kin. Furthermore, the highest cell growth was obtained from the leaf and inflorescence calli transferred to liquid MS medium supplemented with 1 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-D)+1 mg l−1 Kin/BAP. The highest activity of antioxidant enzymes and the lowest protein content was related to the leaf cell suspension cultures in liquid MS medium containing 3 mg l−1 NAA and 3 mg l−1 BAP. The inflorescence cell suspension cultures in the MS medium containing 3 mg l−1 NAA+1 mg l−1 BAP exhibited the highest rutin accumulation (47.536 mg g−1 FW). However, the highest amount of quercetin (8.570 mg g−1 FW) and kaempferol (5.420 mg g−1 FW) accumulation were obtained from the petiole cell suspension cultures in the MS medium with 1 mg l−1 2,4-D +1 mg l−1 Kin.
摘要本研究研究了植物生长调节剂和外植体类型对苦楝愈伤组织诱导、悬浮培养细胞生长、抗氧化酶活性以及芦丁、槲皮素和山奈酚积累的影响。我们的研究表明，与叶片外植体相比，花序和叶柄外植体的愈伤组织诱导率较高，而叶片外植体在含有5 mg l - 1 NAA和5 mg l - 1 BAP以及5 mg l - 1 NAA和3 mg l - 1 kinetin Kin的MS培养基上的愈伤组织生长最高。此外，将叶片和花序愈伤组织转移到添加1 mg l−1 2,4-二氯苯氧乙酸(2,4- d)+1 mg l−1 Kin/BAP的MS液体培养基中，细胞生长最快。在含有3 mg l - 1 NAA和3 mg l - 1 BAP的MS液体培养基中，叶细胞悬浮培养的抗氧化酶活性最高，蛋白质含量最低。在含有3 mg l−1 NAA+1 mg l−1 BAP的MS培养基中，花序细胞的芦丁积累量最高(47.536 mg g−1 FW)。然而，叶柄细胞悬浮培养中槲皮素(8.570 mg g−1 FW)和山奈酚(5.420 mg g−1 FW)积累量最高的是MS培养基(1 mg l−1 2,4- d +1 mg l−1 Kin)。
{"title":"The effect of plant growth regulators on the antioxidant enzyme activity and secondary metabolite production in the cell suspension cultures of Melia azedarach L.","authors":"F. Ahmadpoor, N. Zare, Rasool Asghari, Parisa Sheikhzadeh-Mosadeg","doi":"10.1080/14620316.2023.2185165","DOIUrl":"https://doi.org/10.1080/14620316.2023.2185165","url":null,"abstract":"ABSTRACT In the present study, we investigated the effect of plant growth regulators and explant type on callus induction, cell suspension culture growth, antioxidant enzyme activity, and accumulation of rutin, quercetin, and kaempferol in M. azedarach L. cell cultures. Our studies showed that the inflorescence and petiole explants had a high percentage of callus induction compared to the leaf explants, whereas the highest callus growth was obtained from the leaf explants cultured on the MS medium containing 5 mg l−1 NAA and 5 mg l−1 BAP and 5 mg l−1 NAA and 3 mg l−1 kinetin Kin. Furthermore, the highest cell growth was obtained from the leaf and inflorescence calli transferred to liquid MS medium supplemented with 1 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-D)+1 mg l−1 Kin/BAP. The highest activity of antioxidant enzymes and the lowest protein content was related to the leaf cell suspension cultures in liquid MS medium containing 3 mg l−1 NAA and 3 mg l−1 BAP. The inflorescence cell suspension cultures in the MS medium containing 3 mg l−1 NAA+1 mg l−1 BAP exhibited the highest rutin accumulation (47.536 mg g−1 FW). However, the highest amount of quercetin (8.570 mg g−1 FW) and kaempferol (5.420 mg g−1 FW) accumulation were obtained from the petiole cell suspension cultures in the MS medium with 1 mg l−1 2,4-D +1 mg l−1 Kin.","PeriodicalId":22704,"journal":{"name":"The Journal of Horticultural Science and Biotechnology","volume":"18 1","pages":"662 - 677"},"PeriodicalIF":0.0,"publicationDate":"2023-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82993737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-15DOI: 10.1080/14620316.2023.2183903
Shweta, S. Sood
ABSTRACT In modern agriculture, sustainable production and efficiency are unimaginable without the use of agrochemicals such as pesticides, fertilisers, herbicides, etc. Nanotechnology has the potential to augment food quality, plant protection, detection of plant and animal diseases, monitoring of plant growth, global food production, and seed quality. The potential for a wide range of applications makes a basic understanding of nanotechnology important. This article presents an introduction to nanotechnology and discusses the implications of it for the tomato crop. The review aims to systematise and discuss research data related to the effect of nanomaterials on tomato, an important fruit crop used as a model plant for research which provides knowledge about this fast-developing research area.
{"title":"A review on nanotechnological advancements in tomato: a model plant","authors":"Shweta, S. Sood","doi":"10.1080/14620316.2023.2183903","DOIUrl":"https://doi.org/10.1080/14620316.2023.2183903","url":null,"abstract":"ABSTRACT In modern agriculture, sustainable production and efficiency are unimaginable without the use of agrochemicals such as pesticides, fertilisers, herbicides, etc. Nanotechnology has the potential to augment food quality, plant protection, detection of plant and animal diseases, monitoring of plant growth, global food production, and seed quality. The potential for a wide range of applications makes a basic understanding of nanotechnology important. This article presents an introduction to nanotechnology and discusses the implications of it for the tomato crop. The review aims to systematise and discuss research data related to the effect of nanomaterials on tomato, an important fruit crop used as a model plant for research which provides knowledge about this fast-developing research area.","PeriodicalId":22704,"journal":{"name":"The Journal of Horticultural Science and Biotechnology","volume":"29 1","pages":"563 - 579"},"PeriodicalIF":0.0,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72853964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-07DOI: 10.1080/14620316.2023.2185164
Naveena Kumara K T, H. S. Singh, N. Kaur, Kirandeep Kaur
ABSTRACT The present investigation was aimed at assessing the efficacy of N1-(2-chloro-4-pyridyl)-N3-phenylurea (CPPU) and salicylic acid on fruit retention, yield, and quality of mango cv. Dusehri. The research was carried out at the Department of Fruit Science, Punjab Agricultural University, Ludhiana, India. The experiment was conducted simultaneously at two different locations for two cropping seasons during 2019–20 and 2020–21. Fruit retention enhancing treatments of 2,4-Dichlorophenoxyacetic acid (2,4-D) (@20 ppm), CPPU (@5, 10, and 15 ppm), and salicylic acid (@100, 200, and 300 ppm) were applied at pea stage of fruit growth. Experimental plants were observed for various reproductive, yield, and fruit quality parameters. The results indicated that foliar application of CPPU (T6-CPPU @10 ppm) significantly enhanced fruit retention during marble and harvest stages at both locations in both seasons. The novel growth hormone also improved fruit yield, fruit weight, and fruit quality in mango cv. Dusehri. Foliar applied salicylic acid recorded intermediate values for observed parameters. Therefore, foliar application of CPPU can be considered as a better alternative to 2,4-D for fruit drop management of mango.
{"title":"CPPU and salicylic acid application improved fruit retention, yield, and fruit quality of mango cv. Dusehri","authors":"Naveena Kumara K T, H. S. Singh, N. Kaur, Kirandeep Kaur","doi":"10.1080/14620316.2023.2185164","DOIUrl":"https://doi.org/10.1080/14620316.2023.2185164","url":null,"abstract":"ABSTRACT The present investigation was aimed at assessing the efficacy of N1-(2-chloro-4-pyridyl)-N3-phenylurea (CPPU) and salicylic acid on fruit retention, yield, and quality of mango cv. Dusehri. The research was carried out at the Department of Fruit Science, Punjab Agricultural University, Ludhiana, India. The experiment was conducted simultaneously at two different locations for two cropping seasons during 2019–20 and 2020–21. Fruit retention enhancing treatments of 2,4-Dichlorophenoxyacetic acid (2,4-D) (@20 ppm), CPPU (@5, 10, and 15 ppm), and salicylic acid (@100, 200, and 300 ppm) were applied at pea stage of fruit growth. Experimental plants were observed for various reproductive, yield, and fruit quality parameters. The results indicated that foliar application of CPPU (T6-CPPU @10 ppm) significantly enhanced fruit retention during marble and harvest stages at both locations in both seasons. The novel growth hormone also improved fruit yield, fruit weight, and fruit quality in mango cv. Dusehri. Foliar applied salicylic acid recorded intermediate values for observed parameters. Therefore, foliar application of CPPU can be considered as a better alternative to 2,4-D for fruit drop management of mango.","PeriodicalId":22704,"journal":{"name":"The Journal of Horticultural Science and Biotechnology","volume":"21 1","pages":"608 - 621"},"PeriodicalIF":0.0,"publicationDate":"2023-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81731895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-01DOI: 10.1080/14620316.2023.2185166
B. Çakır, Hamed Jalili, Güneş Turgay
ABSTRACT All living organisms contain ATP-binding cassette (ABC) transporters that perform different biological functions. Here, we identified 19 putative VvABCB from grapevine genomic and proteomic databases. Bioinformatics analyses revealed the phylogenetic relationship, conserved motifs, localisation, and structure of ABCB genes and the regulatory elements in their promoter. Twenty conserved motifs were found among the VvABCB subfamily and their properties were similar to those of ABC transporters. We showed that the VvABCB gene promoters contain many elements related to the light response motif in addition to gibberellin-responsive elements, auxin-responsive elements, and cis-acting element involved in abiotic stresses. The gene expression levels were also analysed at different developmental stages of berries. The transcripts levels of VvABCB genes differed at the various developmental stages of berries, suggesting that they are involved in growth and development. The expression profiles of VvABCB genes obtained via qRT-PCR were used to assess genetic responses to iron and heavy metal stresses. Iron, copper, mercury, and boron induced regulatory responses in VvABCB genes, which likely play a role in coping with iron and heavy metal stresses in plants. This study establishes a base for future functional research on this class of ABC transporters in grapevine.
{"title":"Genome-wide analysis of the ABCB gene family in Vitis vinifera: its expression patterns in berries and its responses to iron and heavy metal stresses","authors":"B. Çakır, Hamed Jalili, Güneş Turgay","doi":"10.1080/14620316.2023.2185166","DOIUrl":"https://doi.org/10.1080/14620316.2023.2185166","url":null,"abstract":"ABSTRACT All living organisms contain ATP-binding cassette (ABC) transporters that perform different biological functions. Here, we identified 19 putative VvABCB from grapevine genomic and proteomic databases. Bioinformatics analyses revealed the phylogenetic relationship, conserved motifs, localisation, and structure of ABCB genes and the regulatory elements in their promoter. Twenty conserved motifs were found among the VvABCB subfamily and their properties were similar to those of ABC transporters. We showed that the VvABCB gene promoters contain many elements related to the light response motif in addition to gibberellin-responsive elements, auxin-responsive elements, and cis-acting element involved in abiotic stresses. The gene expression levels were also analysed at different developmental stages of berries. The transcripts levels of VvABCB genes differed at the various developmental stages of berries, suggesting that they are involved in growth and development. The expression profiles of VvABCB genes obtained via qRT-PCR were used to assess genetic responses to iron and heavy metal stresses. Iron, copper, mercury, and boron induced regulatory responses in VvABCB genes, which likely play a role in coping with iron and heavy metal stresses in plants. This study establishes a base for future functional research on this class of ABC transporters in grapevine.","PeriodicalId":22704,"journal":{"name":"The Journal of Horticultural Science and Biotechnology","volume":"19 1","pages":"591 - 607"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76900734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-24DOI: 10.1080/14620316.2023.2180438
Inti Ganganelli, María Celeste Molina Agostini, A. Galatro, G. E. Gergoff Grozeff
ABSTRACT Light-emitting diodes (LED) have emerged as a non-polluting and energy-saving technology in fruit postharvest. The aim of this work was to evaluate the effect of different LED light pulses during raspberries´ and blackberries´ postharvest under cold storage. Fruit was exposed to short LED light pulses (Green, Blue, Red, and Red+Blue) of 15 min every 2 h for 7 and 14 d of storage at 4°C. Raspberries were more susceptible to water loss. Short LED light pulses decreased internal breakdown in raspberries, and minor effects of LED treatments were observed in blackberries. After 7 d of cold storage, in raspberries, Green, Blue, and Red light treatments increased the content of quinic and malic acids, and only Green and Blue LED sources also increased ascorbic acid content. In addition, Blue, Green, and Blue+Red LED light treatments exhibited a higher level of citric acid at 14 d. In blackberries, fruit changes were mainly because of the cold storage. Overall, these results showed that short LED light pulses can extend the postharvest life of raspberries, decreasing internal breakdown and increasing the content of ascorbic acid, suggesting that different Rubus species have distinct physiological processes underlying ripening and light interactions.
{"title":"Specific wavelength LED light pulses modify vitamin C and organic acids content in raspberry and blackberry fruit during postharvest","authors":"Inti Ganganelli, María Celeste Molina Agostini, A. Galatro, G. E. Gergoff Grozeff","doi":"10.1080/14620316.2023.2180438","DOIUrl":"https://doi.org/10.1080/14620316.2023.2180438","url":null,"abstract":"ABSTRACT Light-emitting diodes (LED) have emerged as a non-polluting and energy-saving technology in fruit postharvest. The aim of this work was to evaluate the effect of different LED light pulses during raspberries´ and blackberries´ postharvest under cold storage. Fruit was exposed to short LED light pulses (Green, Blue, Red, and Red+Blue) of 15 min every 2 h for 7 and 14 d of storage at 4°C. Raspberries were more susceptible to water loss. Short LED light pulses decreased internal breakdown in raspberries, and minor effects of LED treatments were observed in blackberries. After 7 d of cold storage, in raspberries, Green, Blue, and Red light treatments increased the content of quinic and malic acids, and only Green and Blue LED sources also increased ascorbic acid content. In addition, Blue, Green, and Blue+Red LED light treatments exhibited a higher level of citric acid at 14 d. In blackberries, fruit changes were mainly because of the cold storage. Overall, these results showed that short LED light pulses can extend the postharvest life of raspberries, decreasing internal breakdown and increasing the content of ascorbic acid, suggesting that different Rubus species have distinct physiological processes underlying ripening and light interactions.","PeriodicalId":22704,"journal":{"name":"The Journal of Horticultural Science and Biotechnology","volume":"55 1","pages":"649 - 661"},"PeriodicalIF":0.0,"publicationDate":"2023-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82623710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-24DOI: 10.1080/14620316.2023.2179548
M. Manokari, Mahesh Kumar Badhepuri, M. Cokul Raj, A. Dey, M. Faisal, Abdulrahman A Alatar, A. Alok, Phanikanth Jogam, M. Shekhawat
ABSTRACT Gardenia jasminoides J. Ellis (Cape jasmine) is a horticultural plant. It is difficult to cultivate species due to pathogen sensitivity; hence, the demand for this species is gradually increasing. Thus, micropropagation would be a convenient tool to produce quality planting material, but the higher rate of mortality constitutes a constraint to large-scale propagation. Seismic stress is one of the abiotic stresses that promote growth, structural stability, and positively affect plantlet development. The objective of the present study was to evaluate the effect of moderate seismic stress under in vitro conditions on the proliferation of shoots, biochemical attributes (photosynthetic pigments, carbohydrates constituents, free amino acids, etc.), and foliar anatomical stability of plantlets G. jasminoides. Results showed the superiority of moderate seismic stress (75 rpm for 10 min) in the improved proliferation of shoots, development of functional stomata, elimination of anatomical abnormalities, improved photosynthetic pigments and rooting efficiency, and finally increased survival percentage of plantlets after transplantation into field conditions. These findings provided evidence for the positive role of the optimised seismic stress in regulating the morpho-anatomical and biochemical traits in G. jasminoides which is an indication of the development of diverse adaptive strategies by the micropropagated plantlets to abiotic stress.
{"title":"Seismic stress-mediated improvements in morphometry, foliar anatomy and biochemistry of in vitro grown plants of Gardenia jasminoides J. Ellis","authors":"M. Manokari, Mahesh Kumar Badhepuri, M. Cokul Raj, A. Dey, M. Faisal, Abdulrahman A Alatar, A. Alok, Phanikanth Jogam, M. Shekhawat","doi":"10.1080/14620316.2023.2179548","DOIUrl":"https://doi.org/10.1080/14620316.2023.2179548","url":null,"abstract":"ABSTRACT Gardenia jasminoides J. Ellis (Cape jasmine) is a horticultural plant. It is difficult to cultivate species due to pathogen sensitivity; hence, the demand for this species is gradually increasing. Thus, micropropagation would be a convenient tool to produce quality planting material, but the higher rate of mortality constitutes a constraint to large-scale propagation. Seismic stress is one of the abiotic stresses that promote growth, structural stability, and positively affect plantlet development. The objective of the present study was to evaluate the effect of moderate seismic stress under in vitro conditions on the proliferation of shoots, biochemical attributes (photosynthetic pigments, carbohydrates constituents, free amino acids, etc.), and foliar anatomical stability of plantlets G. jasminoides. Results showed the superiority of moderate seismic stress (75 rpm for 10 min) in the improved proliferation of shoots, development of functional stomata, elimination of anatomical abnormalities, improved photosynthetic pigments and rooting efficiency, and finally increased survival percentage of plantlets after transplantation into field conditions. These findings provided evidence for the positive role of the optimised seismic stress in regulating the morpho-anatomical and biochemical traits in G. jasminoides which is an indication of the development of diverse adaptive strategies by the micropropagated plantlets to abiotic stress.","PeriodicalId":22704,"journal":{"name":"The Journal of Horticultural Science and Biotechnology","volume":"91 1","pages":"622 - 634"},"PeriodicalIF":0.0,"publicationDate":"2023-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80377333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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