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QUANTITATIVE MICROBIAL RISK ASSESSMENT OF FERMENTED BEAN PASTES CONTAMINATED WITH CLOSTRIDIUM PERFRINGENS 产气荚膜梭菌污染豆瓣酱的定量微生物风险评价
Pub Date : 2021-09-14 DOI: 10.15414/JMBFS.3854
Y. Seo, Ye-Kyung Lee, Sejeong Kim, Jeeyeon Lee, J. Ha, Yukyung Choi, H. Oh, Yujin Kim, M. Rhee, Heeyoung Lee, Y. Yoon
This study evaluated Clostridium perfringens risk in fermented bean pastes. The prevalence in fermented bean pastes, development of the predictive model, temperature, time, and consumption data were investigated. A simulation model was prepared in @RISK with the obtained data to calculate the probability of foodborne C. perfringens foodborne illness from fermented bean paste consumption. Only 74 of the 1,097 samples were positive for C. perfringens. The initial contamination level was estimated to be 0.7 log CFU/g. It showed the probability of foodborne C. perfringens infection per person per day upon consumption of fermented bean pastes was 8.0×10−12, which is considered low risk.
本研究评估了发酵豆沙中产气荚膜梭菌的风险。对豆瓣酱中的流行程度、预测模型的建立、温度、时间和消费数据进行了调查。利用所得数据在@RISK中建立仿真模型,计算食源性产气荚膜荚膜杆菌食源性疾病的概率。1097份样本中仅有74份产气荚膜梭菌阳性。最初的污染水平估计为0.7 log CFU/g。结果显示,人均每天食用豆豉后食源性产气荚膜荚膜杆菌感染概率为8.0×10−12,属于低风险。
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引用次数: 0
A REVIEW ON UNDERSTANDING OF EGG YOLK AS FUNCTIONAL INGREDIENTS 蛋黄作为功能性配料的认识综述
Pub Date : 2021-09-13 DOI: 10.15414/JMBFS.4627
S. Patil, B. Rao, Malhar Matondkar, Pravin R. Bhushette, S. Sonawane
The various bakery products like cake, cookies, and bread includes egg as an ingredient, which plays significant role in the product development. The egg yolk is the dense yellow part of the egg and is considered nutritionally more beneficial when compared to the other portion of the (egg whites). The egg yolk is considered a rich source of essential fat-soluble vitamins (A, D, E, and K) and few water-soluble vitamins (B6, B12). It is also rich in calcium, magnesium, iron, and selenium. This review discusses about functional characterization like interfacial properties, gelation, and phase separation behavior of egg yolk and its constituents (plasma and granules). This reviews briefly discuss about constituent characterization like xanthophylls, color stability, fatty composition with using newer analysis techniques. Besides, its applications in different industries like bakery, snacks, meat industry and production of nanogels have also been documented.
蛋糕、饼干、面包等各种烘焙产品都含有鸡蛋,鸡蛋在产品开发中起着重要作用。蛋黄是鸡蛋中致密的黄色部分,与蛋清的其他部分相比,蛋黄被认为更有营养。蛋黄被认为是必需脂溶性维生素(a、D、E和K)和少量水溶性维生素(B6、B12)的丰富来源。它还富含钙、镁、铁和硒。本文综述了蛋黄及其成分(血浆和颗粒)的界面特性、凝胶和相分离行为等功能表征。本文就叶黄素、颜色稳定性、脂肪组成等成分的表征及最新的分析技术作一综述。此外,它在烘焙、零食、肉类工业和纳米凝胶生产等不同行业的应用也有文献记载。
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引用次数: 1
THE PROBIOTIC AND NUTRITIONAL CONTENT OF SOURO, AN IRANIAN TRADITIONAL FOOD 酸奶中的益生菌和营养成分,这是一种伊朗传统食品
Pub Date : 2021-09-13 DOI: 10.15414/JMBFS.2627
Milad Mohkam, Shiva Ghasemi Firoozabadi, Y. Ghasemi, A. Gholami
Probiotic foods are products containing adequate amounts of live and specific microorganisms that confer benefit to human health. This research was performed to spotlight on the nutritional value evaluation as well as isolation and probiotic characterization of lactic acid bacteria (LAB) isolated from native food "Souro" belonging to Iran. The isolated LABs were investigated in vitro for potential probiotic features based on their acidic pH tolerance, bile-salt resistance, hydrophobicity, autoaggragation, production of amylase and protease as well as antibiotic susceptibility test. Results showed that Souro had high content of protein (51 ± 0.16%,) and total lipid was 11.18 ± 0.38%. Most of isolates were able to produce protease and amylase. From the best potential probiotic isolates, 16S rRNA gene sequencing disclosed Lactobacillus plantarum, L. hilgardii, L. casei, L. fermentum and Weissella paramesenteroides. Thus, good physiological traits and safety evaluation of the isolated LAB emphasized the benefits of Souro for further use in the human diet as well as the preparation of diverse probiotic food formulations.
益生菌食品是含有足量的活微生物和特定微生物的产品,对人体健康有益。本研究旨在研究从伊朗本土食品“Souro”中分离得到的乳酸菌(LAB)的营养价值评价、分离及益生菌特性。对分离菌株的酸性pH耐受性、抗胆盐性、疏水性、自聚集性、淀粉酶和蛋白酶的产生以及抗生素敏感性进行了体外研究,以确定其潜在的益生菌特性。结果表明,苏鲁蛋白含量为51±0.16%,总脂质含量为11.18±0.38%。大多数菌株能产生蛋白酶和淀粉酶。从最有潜力的益生菌分离株中,16S rRNA基因测序发现了植物乳杆菌、hilgardii乳杆菌、干酪乳杆菌、发酵乳杆菌和副小肠魏氏菌。因此,分离的乳酸菌良好的生理特性和安全性评价强调了Souro在人类饮食中的进一步应用以及制备各种益生菌食品配方的益处。
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引用次数: 2
USE OF BACTERIOPHAGES AS A TARGET SPECIFIC THERAPY AGAINST FOOD-BORNE PATHOGENS IN FOOD INDUSTRY- A REVIEW: Bacteriophage 噬菌体在食品工业中作为食源性致病菌靶向特异性治疗的应用——综述:噬菌体
Pub Date : 2021-09-06 DOI: 10.15414/JMBFS.2949
Fozia Hameed, J. Bandral, N. Gupta, Gulzar Ahmad Nayik, M. Sood, Rukhsana Rahman
Within the context of food safety, the use of modern antimicrobial technologies to inactivate the food-borne pathogens is not infallible, as proven by the ceaseless increase in the food-borne illnesses. The extensive use of antibiotics to minimize the microbial contamination further results in the evolution of antibiotic-resistant bacterial pathogens. Moreover, some of the decontamination methods frequently used in the food industry cannot be directly applied to fresh fruits and vegetables, and RTE meals. Therefore, efforts are being made by researchers in developing a new approach so as to control the bacterial contamination. Consequently, bacteriophages have evolved as the safe, green, effective and natural alternative for treatment and complete destruction of pathogens in food industry.  The review provides a general description about bacteriophages and has mainly focused on their potential use as antimicrobials during the postharvest processing of foods. Numerous research papers, review papers, book chapters and other relevant literature was used for writing this review paper.
在食品安全的背景下,正如食源性疾病不断增加所证明的那样,使用现代抗菌技术来灭活食源性病原体并非万无一失。为了尽量减少微生物污染而广泛使用抗生素,进一步导致耐抗生素细菌病原体的进化。此外,食品工业中经常使用的一些去污方法不能直接应用于新鲜水果和蔬菜,以及RTE餐。因此,研究人员正在努力开发一种新的方法来控制细菌污染。因此,噬菌体已经发展成为食品工业中安全、绿色、有效和天然的治疗和彻底消灭病原体的替代品。本文对噬菌体进行了概述,重点介绍了它们在食品采后加工过程中作为抗菌剂的潜在用途。大量的研究论文、综述论文、书籍章节和其他相关文献被用于撰写这篇综述论文。
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引用次数: 1
PREDICTING SHELF LIFE OF CHICKEN BURGER PRODUCED FROM SURIMI UNDER COMBINED USE OF THYME ESSENTIAL OIL AND MODIFIED ATMOSPHERE PACKAGING: The effect of essential oils on new burgers 百里香精油与气调包装联合用于鱼糜鸡肉汉堡的保质期预测:精油对新汉堡的影响
Pub Date : 2021-09-03 DOI: 10.15414/JMBFS.1662
Azadeh Rashidimehr, A. Fazlara, M. Zarei, M. Pourmehdi, M. Noshad
In the present research, the mixed use of thyme essential oil (EO) and Modified Atmosphere Packaging (MAP) treatments is studied to increase the quality and the shelf life of burgers prepared from surimi and chicken meat which were refrigerated at 2°C for 27 days. Obviously, there was a significant difference (p < 0.001) between chemical and microbiological analyses of burger treated with thyme EO, free of EOs and packed in the air or in the MAP. So burger shelf life was extended by 18 days with the combination of MAP packaging with thyme EO. Also, Artificial Neural Network (ANN) was used to forecast the shelf life of produced burgers under thyme essential oil and MAP influence. The model obtained from ANN has a higher (R2) and less RMSE in predicting the shelf life of produced burgers under the combined use of thyme essential oil and MAP.
在本研究中,研究了百里香精油(EO)和改性气氛包装(MAP)的混合使用,以提高鱼糜和鸡肉在2°C冷藏27天的汉堡的质量和保质期。显然,百里香EO处理的汉堡、不含EO的汉堡、空气包装和MAP包装的汉堡的化学和微生物分析结果有显著差异(p < 0.001)。因此,MAP包装与百里香EO包装相结合可使汉堡的保质期延长18天。利用人工神经网络(ANN)对百里香精油和MAP影响下的汉堡货架期进行预测。人工神经网络模型在预测百里香精油和MAP联合使用下生产的汉堡的保质期方面具有较高的(R2)和较小的RMSE。
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引用次数: 0
THE PHYSICAL AND CHEMICAL PROPERTIES OF THE JUJUBE FRUITS AT DIFFERENT MATURATION STAGES 枣果实在不同成熟期的理化性质
Pub Date : 2021-09-02 DOI: 10.15414/JMBFS.4370
F. Tepe, R. Ekinci, Ayten Ekinci
The effects of maturation stages on some physical and chemical properties of the jujube fruits were investigated. The maturation stages of the jujube fruits were described as green, yellow, half-red and red maturation. The physical properties and chemical composition of the jujube fruit significantly changed depending on the maturation stage. The size and weight of the jujube fruits increased, and the peel color changed from green to red with the maturation. The content of water-soluble vitamins, trans-resveratrol, total phenolic content and antioxidant capacity of the jujube fruits decreased during the maturation. The trans-resveratrol content of the jujube fruits was investigated for the first time and no trans-resveratrol content could be detected at red maturation stage. The major organic acid and sugar at the red maturation stage were found to be malic acid and sucrose, respectively. The phenolic composition also changed, but these changes showed unstable trend during the maturation. The major phenolic compound was catechin at the green maturation stage, whereas chlorogenic acid dominated at the red maturation stage. The jujube fruit is rich in minerals especially potassium and no changes in the mineral contents except for copper was observed between green and red maturation stages.
研究了不同成熟阶段对枣果实理化性质的影响。红枣果实的成熟阶段分为青熟、黄熟、半红熟和红熟。红枣果实的物理性质和化学成分随成熟期的不同而发生显著变化。红枣果实的大小和重量随着成熟而增加,果皮颜色由绿色变为红色。红枣果实的水溶性维生素含量、反式白藜芦醇含量、总酚含量和抗氧化能力随着成熟而降低。首次对红枣果实的反式白藜芦醇含量进行了测定,在红枣成熟阶段未检测到反式白藜芦醇的含量。红色成熟期的主要有机酸和糖分别为苹果酸和蔗糖。成熟过程中酚类成分也发生了变化,但变化趋势不稳定。绿色成熟期的主要酚类化合物为儿茶素,而红色成熟期的主要酚类化合物为绿原酸。红枣果实中含有丰富的矿物质,尤其是钾元素,除铜元素外,红枣果实绿熟期和红熟期的矿物质含量没有变化。
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引用次数: 0
EXTRACELLULAR GLUTAMINASE-FREE L-ASPARAGINASE FROM TRICHODERMA VIRIDE F2: PURIFICATION, BIOCHEMICAL CHARACTERIZATION AND EVALUATION OF ITS POTENTIAL IN MITIGATING ACRYLAMIDE FORMATION IN STARCHY FRIED FOOD 绿木霉胞外无谷氨酰胺酶l -天冬酰胺酶f2:纯化、生化表征及其在淀粉油炸食品中抑制丙烯酰胺形成的潜力评价
Pub Date : 2021-06-08 DOI: 10.15414/JMBFS.4336
A. Elshafei, D. H. El-Ghonemy
L-asparaginase is an antitumor agent that suppresses cancer cell growth by eliminating L-asparagine from malignant cells. However, the intrinsic glutaminase activity is responsible for significant life-threatening adverse effects. Therefore, glutaminase-free L-asparaginase is far required to improve the therapeutic efficacy of L-asparaginase treatment. L-asparaginase was also used to combat the development of acrylamide in foods rich in carbohydrates cooked at high temperatures. Therefore, this study explores the purification and characterization of glutaminase-free L-asparaginase from Trichoderma viride F2 using agro-industrial residues as substrate. The enzyme was purified 36-folds with 688.1 U/mg specific activity and a final yield of 38.9% through ethanol precipitation, gel filtration on Sephadex G-100 followed by Sephadex G-200. The purified L-asparaginase is monomeric with a molecular mass of 57 kDa and exhibited optimum activity at pH 7.5 and 37 °C, which is relatively close to the human body's internal environment. The purified L-asparaginase showed high affinity and catalytic efficiency towards its natural substrate L-asparagine with Km and Vmax of 1.2 mM and 71.3 U/mL, respectively, and did not exhibit any intrinsic glutaminase activity. Among the salts tested, the univalent cations Na+ and K+ enhanced the activity by 145.7% and 163.5%, respectively, while the presence of Ag+ and Fe+3 displayed a considerable loss in activity. The enzyme showed a good anti-oxidant activity with IC50 of 66.1μg/mL and was able to convert L-asparagine exist in potatoes to L-aspartic acid and ammonia, suggesting its use as anti-carcinogenic agent and as potential food industry candidate to mitigate acrylamide content in starchy fried food.
l -天冬酰胺酶是一种抗肿瘤药物,通过消除恶性细胞中的l -天冬酰胺来抑制癌细胞的生长。然而,内在的谷氨酰胺酶活性是负责重大的危及生命的不良反应。因此,需要无谷氨酰胺酶的l -天冬酰胺酶来提高l -天冬酰胺酶治疗的疗效。l -天冬酰胺酶也被用来对抗高温烹饪的富含碳水化合物的食物中丙烯酰胺的形成。因此,本研究以农业工业残留物为底物,从绿色木霉F2中纯化和表征无谷氨酰胺酶的l -天冬酰胺酶。经乙醇沉淀、Sephadex G-100凝胶过滤、Sephadex G-200凝胶过滤,得到36倍纯化,比活性为688.1 U/mg,最终得率为38.9%。纯化得到的l -天冬酰胺酶为单体,分子量为57 kDa,在pH 7.5和37℃条件下具有最佳活性,相对接近人体内环境。纯化后的l -天冬酰胺酶对天然底物l -天冬酰胺具有较高的亲和力和催化效率,Km和Vmax分别为1.2 mM和71.3 U/mL,不表现出任何内在的谷氨酰胺酶活性。其中,一价阳离子Na+和K+分别提高了145.7%和163.5%的活性,而Ag+和Fe+3的存在则使活性明显降低。该酶具有良好的抗氧化活性,IC50值为66.1μg/mL,能将马铃薯中的l -天冬酰胺转化为l -天冬氨酸和氨,表明其具有抗癌作用,可作为降低淀粉油炸食品中丙烯酰胺含量的潜在候选物。
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引用次数: 3
TAXONOMIC INVESTIGATION OF EUPLANKTONIC DIATOM COMMUNITIES AS INDICATOR OF COPPER IN THE BANK OF THE SUBARNAREKHA RIVER, GHATSHILA, JHARKHAND, INDIA 印度恰尔肯德邦加特希拉subarnaarekha河河岸富浮游硅藻群落作为铜指示物的分类学研究
Pub Date : 2021-04-08 DOI: 10.15414/JMBFS.2827
G. G. Satpati, Rahul Bose, R. Pal
The aim of this study was to demonstrate and evaluate the diatom communities in the copper infected areas readily associated with the Hindustan Copper Limited (HCL) at the bank of the Subarnarekha River. This study was based on three sampling sites commonly designated as high copper (>100 μg.L-1), medium copper (≤100 μg.L-1) and low copper (≤50 μg.L-1) contaminated area. Results indicated the detailed taxonomic description of 31 species that are dominant or less dominant over these contaminated area. Among the identified taxa, 10 were recorded as new to the Jharkhand state. Water analysis has suggested the presence of 17 species in the high copper contaminated area adjacent to HCL. Nine species was less dominant in the outlet of HCL that belonged to the medium contaminated and only 5 species were dominant over the low copper contaminated area. Physico-chemical parameters like pH, air and water temperature, salinity, conductivity, light extinction coefficient, turbidity, dissolved inorganic salts, dissolved oxygen and carbon-di-oxide, biological oxygen demand and total hardness were also estimated in the copper contaminated sites. Relatively all the species of Cymbella and Navicula were associated with high copper accumulation. Most interestingly, one harmful species Halamphora coffeiformis, which was recorded as most dominant species in high copper exposed area, has shown to be the best copper tolerant and copper indicator species.
本研究的目的是证明和评价在苏巴纳列卡河岸边与印度斯坦铜业有限公司(HCL)相关的铜感染地区的硅藻群落。本研究选取了高铜(100 μg.L-1)、中铜(≤100 μg.L-1)和低铜(≤50 μg.L-1)污染区3个采样点。结果表明,在污染区内具有优势或不太优势的31种植物有详细的分类描述。在已确定的分类群中,有10个是贾坎德邦的新记录。水分析表明,在HCL附近的高铜污染区存在17种。中等污染的HCL出口有9种优势较少,低铜污染区只有5种优势。对铜污染场地的pH、空气和水温、盐度、电导率、消光系数、浊度、溶解无机盐、溶解氧和二氧化碳、生物需氧量和总硬度等理化参数进行了估算。相对而言,所有的Cymbella和Navicula都有较高的铜积累。最有趣的是,在高铜暴露区,最具优势的有害物种是铜耐受性最好的物种和铜指示剂。
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引用次数: 0
Plant hormones physiological role, effect on human health and its analysis 植物激素的生理作用、对人体健康的影响及其分析
Pub Date : 2021-04-08 DOI: 10.15414/JMBFS.1147
S. Guleria, Mukul Kumar, R. Kaushik
Plant hormones are major growth regulating hormones (auxins, the gibberellins (GAs), the cytokinins) which are easily available in plant and some plant hormones are also worked as a growth inhibitor such as ethylene, abscisic acid (ABA). These hormones showed an effective result in plant growth, flowering, fruiting, aging, senescence, color enhancement of fruits, prevention or promotion of stem elongation, leafing, leaf fall, shoot, root. These plant hormones are misused for an increase in fruit production, fruit ripening, improving fruit quality traits and overall crop improvement. Therefore, there is a need to check the overdoses of these hormones in fresh and processed food products as there higher doses result in several disorders in humans. There limit of use should be specified and there is a need to develop specific analysis methods to detect their limit in agriculture and food products.
植物激素是植物体内容易获得的主要生长调节激素(生长素、赤霉素、细胞分裂素),一些植物激素也作为生长抑制剂,如乙烯、脱落酸(ABA)等。这些激素对植物生长、开花、结果、衰老、衰老、果实增色、防止或促进茎伸长、叶片、落叶、梢、根等均有显著效果。这些植物激素被滥用于增加果实产量、果实成熟、改善果实品质性状和整体作物改良。因此,有必要检查新鲜食品和加工食品中这些激素的过量,因为较高的剂量会导致人体几种疾病。应规定其使用限度,并有必要制定具体的分析方法来检测其在农业和食品中的使用限度。
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引用次数: 1
INFLUENCE OF QUERCETIN AND DIHYDROQUERCETIN ON SOME FUNCTIONAL PARAMETERS OF RAT LIVER MITOCHONDRIA 槲皮素和二氢槲皮素对大鼠肝脏线粒体某些功能参数的影响
Pub Date : 2021-04-07 DOI: 10.15414/JMBFS.2924
U. Gayibov, Sabina Gayibova, M. Pozilov, Feruza Sh. Tuxtaeva, U. R. Yusupova, Gulchehra M.-K. Djabbarova, Zulayho A. Mamatova, N. Ergashev, T. Aripov
Flavonoids are proved to be prominent plant-derived compounds generally found in fruit- and vegetable- rich diets with free radicals quenching ability. Quercetin, a member of flavonoids subclass – flavonols, has gained major attention due to its overwhelming potential. Quercetin was demonstrated to preserve diverse degenerative diseases by inhibiting lipid peroxidation. In the present paper, we deal with two polyphenolic compounds’ – quercetin and dihydroquercetin – influence on functional parameters of mitochondria. Thus, the fore mentioned flavonols showed the mitochondrial permeability transition pore (mPTP) inhibition and ATP-sensitive potassium channel activation capacities as well as strong antioxidаnt/antiradiсal activity. Improving functional parameters of mitochondria by both quercetin and dihydroquercetin formed a basic idea of the hypothesis that the studied flavonols may provide antihypoxic defense.
黄酮类化合物是一种重要的植物源性化合物,普遍存在于富含水果和蔬菜的饮食中,具有自由基猝灭能力。槲皮素作为黄酮亚类黄酮醇的一员,因其巨大的潜力而受到广泛关注。槲皮素被证明通过抑制脂质过氧化来保护多种退行性疾病。本文研究了槲皮素和二氢槲皮素这两种多酚类化合物对线粒体功能参数的影响。因此,上述黄酮醇具有线粒体通透性过渡孔(mPTP)抑制和atp敏感的钾通道激活能力,以及较强的抗氧化/抗辐射活性。槲皮素和二氢槲皮素对线粒体功能参数的改善为所研究的黄酮醇可能具有抗缺氧防御作用的假设提供了基本思路。
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引用次数: 3
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The Journal of Microbiology, Biotechnology and Food Sciences
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