Varish Ahmad, Abrar Ahmad, M. I. Khan, O. Baothman, M. J. Khan
Different recalcitrant aliphatic and aromatic sulfur compounds e.g. Dibenzothiophene, Benzothiophene, and Di-methyl Dibenzothiophene etc. have been remaining in the petrol and diesel, even after hydrodesulfurization, a chemical method of sulfur impurities removal. These sulfur impurities are required to be removed before it is being transported as fuel. So many microbes have been reported in the last decade showing potential for removing the sulfur impurities by using various pathways at ambient temperature, especially 4S pathways. In this study, we describe desulfurization of a model compound Benzothiophene by a Gordonia sp. IITR100 using microbial cell culture and analytical techniques e.g. spectrophotometry and gas chromatography-mass spectrophotometry using column-5. Gordonia sp. IITR100 can grow on Benzothiophene as well as Dibenzothiophene us shown by spectrophotometrical analysis as evident by increased turbidity in aqueous culture. Benzothiophene was metabolized to o-hydroxystyrene as the final product analogous to ‘4S’ pathway as Gas Chromatography-Mass Spectra analysis revealed. Gordonia sp. IITR100 have already been shown desulfurization with heavy organosulfur compounds like Thianthrene, and Benzonahpthothiophene to o-hydroxyphenyl phenylsulfone and 2-phenyl-naphthalene-1-ol respectively. The Gordonia sp. IITR100 have shown potential for Desulfurization of heavy and calcitarant sulfur-compounds suggests its potential (broad substrates range) for use in various tough organosulfur fractions in petroleum. Thus, this bacterial strain could be utilized for commercial purpose of biodesulfurization.
{"title":"A DESULFURIZATION OF BENZOTHIOPHENE BY AN ISOLATED GORDONIA SP. IITR100","authors":"Varish Ahmad, Abrar Ahmad, M. I. Khan, O. Baothman, M. J. Khan","doi":"10.15414/JMBFS.2787","DOIUrl":"https://doi.org/10.15414/JMBFS.2787","url":null,"abstract":"Different recalcitrant aliphatic and aromatic sulfur compounds e.g. Dibenzothiophene, Benzothiophene, and Di-methyl Dibenzothiophene etc. have been remaining in the petrol and diesel, even after hydrodesulfurization, a chemical method of sulfur impurities removal. These sulfur impurities are required to be removed before it is being transported as fuel. So many microbes have been reported in the last decade showing potential for removing the sulfur impurities by using various pathways at ambient temperature, especially 4S pathways. In this study, we describe desulfurization of a model compound Benzothiophene by a Gordonia sp. IITR100 using microbial cell culture and analytical techniques e.g. spectrophotometry and gas chromatography-mass spectrophotometry using column-5. Gordonia sp. IITR100 can grow on Benzothiophene as well as Dibenzothiophene us shown by spectrophotometrical analysis as evident by increased turbidity in aqueous culture. Benzothiophene was metabolized to o-hydroxystyrene as the final product analogous to ‘4S’ pathway as Gas Chromatography-Mass Spectra analysis revealed. Gordonia sp. IITR100 have already been shown desulfurization with heavy organosulfur compounds like Thianthrene, and Benzonahpthothiophene to o-hydroxyphenyl phenylsulfone and 2-phenyl-naphthalene-1-ol respectively. The Gordonia sp. IITR100 have shown potential for Desulfurization of heavy and calcitarant sulfur-compounds suggests its potential (broad substrates range) for use in various tough organosulfur fractions in petroleum. Thus, this bacterial strain could be utilized for commercial purpose of biodesulfurization.","PeriodicalId":22746,"journal":{"name":"The Journal of Microbiology, Biotechnology and Food Sciences","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86721322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Abdelgayed, Alaa F. Bakr, O. El-tawil, A. Bakeer
Ginger is one of most important medicinal plants which used in variable diseases. Recently, ginger nanoparticles have been isolated from ginger extract and exhibited anti-apoptotic activity, hepatoprotective effect against alcohol and may effectively reduce colitis. Our study aimed to compare between the effect of ginger extract and ginger nanoparticles at concentration of 60 µg/ml against hepatotoxicity caused by acetaminophen (APAP; N-acetyl-para-aminophenol) at concentration of 0.1 mg/ml using primary isolated rat hepatocytes. Cytotoxicity was determined by assessing cell viability and leakage of cytosolic enzymes, such as (ALT& AST). Oxidative stress was investigated by measuring levels of MDA and catalase enzymes. The cytopathological lesions were detected by light microscope. Exposure of isolated rat hepatocytes to APAP caused cytotoxicity and oxidative injury, manifested by loss of cell viability and significant increase of enzymes leakages. Also, APAP caused progressive depletion of catalase content and increase in intracellular MDA accumulation, in addition to alteration in histological structures of the cells. Pretreatment of hepatocytes with either GE or GNPs ameliorated the hepatotoxicity, oxidative stress and enzymatic leakage induced by APAP. However, GNPs were more effective compared to ginger extract pre-treated hepatocytes.
{"title":"CYTOLOGICAL STUDY ON RAT ISOLATED HEPATOCYTES AND POSSIBLE PROTECTION OF GINGER (EXTRACT AND NANOPARTICLES) AGAINST ACETAMINOPHEN TOXICITY","authors":"S. Abdelgayed, Alaa F. Bakr, O. El-tawil, A. Bakeer","doi":"10.15414/JMBFS.2681","DOIUrl":"https://doi.org/10.15414/JMBFS.2681","url":null,"abstract":"Ginger is one of most important medicinal plants which used in variable diseases. Recently, ginger nanoparticles have been isolated from ginger extract and exhibited anti-apoptotic activity, hepatoprotective effect against alcohol and may effectively reduce colitis. Our study aimed to compare between the effect of ginger extract and ginger nanoparticles at concentration of 60 µg/ml against hepatotoxicity caused by acetaminophen (APAP; N-acetyl-para-aminophenol) at concentration of 0.1 mg/ml using primary isolated rat hepatocytes. Cytotoxicity was determined by assessing cell viability and leakage of cytosolic enzymes, such as (ALT& AST). Oxidative stress was investigated by measuring levels of MDA and catalase enzymes. The cytopathological lesions were detected by light microscope. Exposure of isolated rat hepatocytes to APAP caused cytotoxicity and oxidative injury, manifested by loss of cell viability and significant increase of enzymes leakages. Also, APAP caused progressive depletion of catalase content and increase in intracellular MDA accumulation, in addition to alteration in histological structures of the cells. Pretreatment of hepatocytes with either GE or GNPs ameliorated the hepatotoxicity, oxidative stress and enzymatic leakage induced by APAP. However, GNPs were more effective compared to ginger extract pre-treated hepatocytes.","PeriodicalId":22746,"journal":{"name":"The Journal of Microbiology, Biotechnology and Food Sciences","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75728046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nikola Lipková, R. Cinkocki, J. Maková, Juraj Medo, S. Javoreková
Bacteria with positive properties on plant vitality are also called PGPB (Plant Growth Promoting Bacteria). Their presence can be observed not only in the root area but also in the above-ground parts of plants like endophytic bacteria. The aim of our study was to characterize promoting features of bacteria from Bacillus genus and compare them with Pseudomonas simiae WCS417 (plant growth promoting strain). The work was carried out in locality Koliňany near Nitra (40°26´46´´N, 79°58´56´´W) and root samples were taken from 6 randomly selected plants of maize (Zea mays L.) in vegetative plant growth stage BBCH 14-15. Bacteria isolated from plant roots were identified and tested to biochemical parameters. From the biochemical features, we observed the detection of siderophores, determination of indole-3-acetic acid (IAA), monitoring the ability to dissolve phosphates and antifungal activity. Bacterial suspensions were applied to maize seeds and tested in vivo controlled conditions. Tested isolates were identified as Bacillus flexus, Bacillus megaterium and Bacillus subtilis. All 3 strains achieved the middle – class of phosphate solubilization index (2.00 ≤ SI ˂ 4.00), produced phytohormone IAA and showed positive production of siderophores and inhibited growth of Fusarium culmorum to more than 50%. All differences between tested strains and control strain P. simiae WCS417 were also statistically confirmed. All strains showed positive results in monitoring plant growth promoting properties. The effect of three Bacillus strains on maize seeds in vivo conditions showed significant differences in root length (P<0.0001) and weight of the young plant (P<0.001) compared to control.
{"title":"CHARACTERIZATION OF ENDOPHYTIC BACTERIA OF THE GENUS BACILLUS AND THEIR INFLUENCE ON THE GROWTH OF MAIZE (ZEA MAYS) IN VIVO","authors":"Nikola Lipková, R. Cinkocki, J. Maková, Juraj Medo, S. Javoreková","doi":"10.15414/JMBFS.3602","DOIUrl":"https://doi.org/10.15414/JMBFS.3602","url":null,"abstract":"Bacteria with positive properties on plant vitality are also called PGPB (Plant Growth Promoting Bacteria). Their presence can be observed not only in the root area but also in the above-ground parts of plants like endophytic bacteria. The aim of our study was to characterize promoting features of bacteria from Bacillus genus and compare them with Pseudomonas simiae WCS417 (plant growth promoting strain). The work was carried out in locality Koliňany near Nitra (40°26´46´´N, 79°58´56´´W) and root samples were taken from 6 randomly selected plants of maize (Zea mays L.) in vegetative plant growth stage BBCH 14-15. Bacteria isolated from plant roots were identified and tested to biochemical parameters. From the biochemical features, we observed the detection of siderophores, determination of indole-3-acetic acid (IAA), monitoring the ability to dissolve phosphates and antifungal activity. Bacterial suspensions were applied to maize seeds and tested in vivo controlled conditions. Tested isolates were identified as Bacillus flexus, Bacillus megaterium and Bacillus subtilis. All 3 strains achieved the middle – class of phosphate solubilization index (2.00 ≤ SI ˂ 4.00), produced phytohormone IAA and showed positive production of siderophores and inhibited growth of Fusarium culmorum to more than 50%. All differences between tested strains and control strain P. simiae WCS417 were also statistically confirmed. All strains showed positive results in monitoring plant growth promoting properties. The effect of three Bacillus strains on maize seeds in vivo conditions showed significant differences in root length (P<0.0001) and weight of the young plant (P<0.001) compared to control.","PeriodicalId":22746,"journal":{"name":"The Journal of Microbiology, Biotechnology and Food Sciences","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72922544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. L. Castrillo, G. A. Bich, N. Amerio, María Daniela Rodríguez, P. Zapata, L. Villalba
The bioconversion of lignocellulosic biomass into monomeric sugars is a key economic difficulty hindering the profitable use of plant biomass as energy. The production of cellulase is a main factor in the cellulose hydrolysis. Among the main cellulase producers are the filamentous fungi. Therefore, many efforts have been made in obtaining new microorganisms with high cellulase secretion capacity. The cellulase secretory capacity of 28 isolates of Trichoderma was qualitatively and quantitatively evaluated. The detection of cellulolytic fungi was correlated with both Congo red and the dinitrosalicylic acid reagent methods. Based on qualitatively assays, sixteen of the isolates revealed carboxymethyl cellulose degradation ability, where the Trichoderma POS7 isolate showed the highest increase in filter paper activity, endo-1,4-β-glucanases and β-glucosidases activities (p<0.05) in a short incubation time. This isolate was molecularly identified as Trichoderma koningiopsis, based on internal transcribed spacer sequences. Our results provide new information and reveal new microorganism in the hydrolysis of cellulose material. The phylogenetic analysis revealed close positioning of T. koningiopsis clade with T. viride, T. viridescens and T. petersenii clades in a closely related group, in concordance with the current taxonomic classification of Trichoderma genus.
{"title":"ASSESSMENT OF CELLULASE COMPLEX SECRETORY CAPACITY OF TRICHODERMA STRAINS AND MORPHOLOGICAL AND MOLECULAR IDENTIFICATION OF THE ISOLATE WITH THE HIGHEST ENZYMATIC SECRETION CAPACITY","authors":"M. L. Castrillo, G. A. Bich, N. Amerio, María Daniela Rodríguez, P. Zapata, L. Villalba","doi":"10.15414/JMBFS.1357","DOIUrl":"https://doi.org/10.15414/JMBFS.1357","url":null,"abstract":"The bioconversion of lignocellulosic biomass into monomeric sugars is a key economic difficulty hindering the profitable use of plant biomass as energy. The production of cellulase is a main factor in the cellulose hydrolysis. Among the main cellulase producers are the filamentous fungi. Therefore, many efforts have been made in obtaining new microorganisms with high cellulase secretion capacity. The cellulase secretory capacity of 28 isolates of Trichoderma was qualitatively and quantitatively evaluated. The detection of cellulolytic fungi was correlated with both Congo red and the dinitrosalicylic acid reagent methods. Based on qualitatively assays, sixteen of the isolates revealed carboxymethyl cellulose degradation ability, where the Trichoderma POS7 isolate showed the highest increase in filter paper activity, endo-1,4-β-glucanases and β-glucosidases activities (p<0.05) in a short incubation time. This isolate was molecularly identified as Trichoderma koningiopsis, based on internal transcribed spacer sequences. Our results provide new information and reveal new microorganism in the hydrolysis of cellulose material. The phylogenetic analysis revealed close positioning of T. koningiopsis clade with T. viride, T. viridescens and T. petersenii clades in a closely related group, in concordance with the current taxonomic classification of Trichoderma genus.","PeriodicalId":22746,"journal":{"name":"The Journal of Microbiology, Biotechnology and Food Sciences","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80037377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Aslam, S. Iqbal, Hafiza Bushra T ariq, Muhammad Imran, M. Rebezov, V. Chumakov, Nadezhda Kenijz, M. Shariati
Interesterification is an alternative to partial hydrogenation that helps to manufacture products of a variety of melting properties. When the process is applied to different blends of vegetable oils, redistribution of fatty acids between and within triacyglycerol occurs that changes the physical properties especially the melting characteristics of the vegetable oil blends. For chemical interesterification process, unrefined vegetable oils (soybean oil and cottonseed oils) were obtained from the local industry (United Industries Faisalabad, Pakistan). Different blends of soybean and cottonseed oils were prepared ranging from 100% soybean oil to 100% cottonseed oil in 25% increments (w/w) with different ratios of (100% : 0%, 75% : 25%, 50% : 50%, 25% : 75%, 0% : 100%) respectively. The blended oil portions (500mL) were neutralized and dried. For chemical interesterification sodium methoxide was used as catalyst. At the end of reaction, the catalyst was inactivated and reaction was stopped by the addition of citric acid solution and at the end by the addition of warm water (70-80°C). Interesterified oils were bleached by heating them to 110°C and mixing with bleaching earth and then the hot interesterified oils were filtered and cooled. Physico-chemical properties (refractive index, specific gravity, colour, melting point, free fatty acids, iodine value, peroxide value, saponification value and rancidity) and sensory evaluation (appearance, odor and overall acceptability) of the final product was conducted at 0, 15 and 30 days of interval. The results revealed that refractive index, specific gravity, color, melting point, free fatty acids value, peroxide value and saponification value increased while iodine value decreased with the passage of time. Further studies showed that storage had a highly significant effect on color, melting point, free fatty acids value, iodine value, peroxide value and saponification value. The effect of storage was non-significant for parameters like specific gravity and refractive index.
{"title":"PHYSICOCHEMICAL PROPERTIES OF CHEMICALLY INTERESTERIFIED VEGETABLE OILS","authors":"F. Aslam, S. Iqbal, Hafiza Bushra T ariq, Muhammad Imran, M. Rebezov, V. Chumakov, Nadezhda Kenijz, M. Shariati","doi":"10.15414/JMBFS.4291","DOIUrl":"https://doi.org/10.15414/JMBFS.4291","url":null,"abstract":"Interesterification is an alternative to partial hydrogenation that helps to manufacture products of a variety of melting properties. When the process is applied to different blends of vegetable oils, redistribution of fatty acids between and within triacyglycerol occurs that changes the physical properties especially the melting characteristics of the vegetable oil blends. For chemical interesterification process, unrefined vegetable oils (soybean oil and cottonseed oils) were obtained from the local industry (United Industries Faisalabad, Pakistan). Different blends of soybean and cottonseed oils were prepared ranging from 100% soybean oil to 100% cottonseed oil in 25% increments (w/w) with different ratios of (100% : 0%, 75% : 25%, 50% : 50%, 25% : 75%, 0% : 100%) respectively. The blended oil portions (500mL) were neutralized and dried. For chemical interesterification sodium methoxide was used as catalyst. At the end of reaction, the catalyst was inactivated and reaction was stopped by the addition of citric acid solution and at the end by the addition of warm water (70-80°C). Interesterified oils were bleached by heating them to 110°C and mixing with bleaching earth and then the hot interesterified oils were filtered and cooled. Physico-chemical properties (refractive index, specific gravity, colour, melting point, free fatty acids, iodine value, peroxide value, saponification value and rancidity) and sensory evaluation (appearance, odor and overall acceptability) of the final product was conducted at 0, 15 and 30 days of interval. The results revealed that refractive index, specific gravity, color, melting point, free fatty acids value, peroxide value and saponification value increased while iodine value decreased with the passage of time. Further studies showed that storage had a highly significant effect on color, melting point, free fatty acids value, iodine value, peroxide value and saponification value. The effect of storage was non-significant for parameters like specific gravity and refractive index.","PeriodicalId":22746,"journal":{"name":"The Journal of Microbiology, Biotechnology and Food Sciences","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85724928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A two-year field study was conducted at El-Ismailiya, Egypt, to evaluate the fungicides Bellis and Sulphurs applied as a foliar sprays for controlling powdery mildew on six flax cultivars. Disease severity, straw yield, and seed yield were used as criteria for evaluating the performance of fungicide on the tested cultivars. Analysis of variance (ANOVA) showed that each of the fungicides and cultivar was highly significant source of variation (p = 0.000), in disease severity, strae yield, and seed yieled. ANOVA also showed that fungicide x cultivars interaction was a highly significant source of variation (p = 0.000) in disease severity, while it was insignificant in straw and seed yields. Both fungicides were effective in reducing disease severity; however, sulphur surpassed Bellis in increasing straw yield and seed yield. Therefore, linear regression analysis was used to determine the relationship between agronomic or technological traits and powdery mildew severity (PMS). All traits showed significant negative correlations with PMS after the appliction of fungicides, which suggest that control of late-season powdery mildew could be economically important when PMS on flax cultivars is high.
{"title":"EFFECT OF CULTIVAR ON THE EFFICIENCY OF FUNGICIDES IN CONTROLLING POWDERY MILDEW OF FLAX AND RELATIONSHIP OF AGRONOMIC AND TECHNOLOGICAL TRAITS TO DISEASE SEVERITY FUNGICIDES FOR CONTROLLING POWDERY MILDEW OF FLAX","authors":"M. Alghuthaymi, A. Aly, A. Amal, K. Abd-Elsalam","doi":"10.15414/JMBFS.2152","DOIUrl":"https://doi.org/10.15414/JMBFS.2152","url":null,"abstract":"A two-year field study was conducted at El-Ismailiya, Egypt, to evaluate the fungicides Bellis and Sulphurs applied as a foliar sprays for controlling powdery mildew on six flax cultivars. Disease severity, straw yield, and seed yield were used as criteria for evaluating the performance of fungicide on the tested cultivars. Analysis of variance (ANOVA) showed that each of the fungicides and cultivar was highly significant source of variation (p = 0.000), in disease severity, strae yield, and seed yieled. ANOVA also showed that fungicide x cultivars interaction was a highly significant source of variation (p = 0.000) in disease severity, while it was insignificant in straw and seed yields. Both fungicides were effective in reducing disease severity; however, sulphur surpassed Bellis in increasing straw yield and seed yield. Therefore, linear regression analysis was used to determine the relationship between agronomic or technological traits and powdery mildew severity (PMS). All traits showed significant negative correlations with PMS after the appliction of fungicides, which suggest that control of late-season powdery mildew could be economically important when PMS on flax cultivars is high.","PeriodicalId":22746,"journal":{"name":"The Journal of Microbiology, Biotechnology and Food Sciences","volume":"1063 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77268569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sally Ibrahim Abd Elfatah, M. Abdel-Kader, N. S. El-Mougy, K. M. Soliman
Maize is the one of important crops in Egypt. Aflatoxins (AFs) are the foremost cancer present compounds by Aspergillus flavus (A. flavus) and cause health risks to human and animals. This study aimed to suppression of aflatoxins production by A. flavus by using different concentrations of natural substances (carnation oil, lemongrass oil, propolis and beewax) and chemical substances (salicylic acid and potassium sorbate) on maize grains. The strains of A. flavus were isolated from local maize grains on Potato Dextrose Agar (PDA) and detect its ability of aflatoxins production on coconut agar media. Samples (100g) of sterilized maize grains were treated individually with different concentration of carnation oil, lemongrass oil, salicylic acid and potassium sorbate (0.25, 0.5, 1.0, 2.0, 4.0 and 6.0%) and at concentration 1% and 4% for propolis and beeswax each, then inoculated with A. flavus and stored for 30 days at 28±2˚C. AFs reduction was determined by using High Performance liquid Chromatography (HPLC). All the tested substances had active effect in inhibition of AFs production by A. flavus in stored maize grains. The production of Aflatoxin B1 and B2 (AFB1 and AFB2) was decreased to about 93% and 99% at concentration of 0.25% carnation oil. Lemongrass oil almost completely inhibited AFB1and AFB2 production (99.12% - 99.98% and 99.98% -99.99%, respectively) at concentration of 2% - 6%. Potassium sorbate and salicylic acid (0.25%- 6%) that were significantly effective controlling aflatoxins production on maize grains compared with control. While, the propolis and beewax found to be the most active to protect maize grains against fungi. Natural substances, carnation oil, lemongrass oil, beewax and propolis had higher active effect at low concentration on aflatoxin production more than salicylic acid and more safe for human used.
{"title":"SUPPRESSION OF AFLATOXINS PRODUCTION IN ARTIFICIALLY INFESTED MAIZE GRAINS WITH ASPERGILLUS FLAVUS DURING STORAGE CONDITIONS","authors":"Sally Ibrahim Abd Elfatah, M. Abdel-Kader, N. S. El-Mougy, K. M. Soliman","doi":"10.15414/JMBFS.2243","DOIUrl":"https://doi.org/10.15414/JMBFS.2243","url":null,"abstract":"Maize is the one of important crops in Egypt. Aflatoxins (AFs) are the foremost cancer present compounds by Aspergillus flavus (A. flavus) and cause health risks to human and animals. This study aimed to suppression of aflatoxins production by A. flavus by using different concentrations of natural substances (carnation oil, lemongrass oil, propolis and beewax) and chemical substances (salicylic acid and potassium sorbate) on maize grains. The strains of A. flavus were isolated from local maize grains on Potato Dextrose Agar (PDA) and detect its ability of aflatoxins production on coconut agar media. Samples (100g) of sterilized maize grains were treated individually with different concentration of carnation oil, lemongrass oil, salicylic acid and potassium sorbate (0.25, 0.5, 1.0, 2.0, 4.0 and 6.0%) and at concentration 1% and 4% for propolis and beeswax each, then inoculated with A. flavus and stored for 30 days at 28±2˚C. AFs reduction was determined by using High Performance liquid Chromatography (HPLC). All the tested substances had active effect in inhibition of AFs production by A. flavus in stored maize grains. The production of Aflatoxin B1 and B2 (AFB1 and AFB2) was decreased to about 93% and 99% at concentration of 0.25% carnation oil. Lemongrass oil almost completely inhibited AFB1and AFB2 production (99.12% - 99.98% and 99.98% -99.99%, respectively) at concentration of 2% - 6%. Potassium sorbate and salicylic acid (0.25%- 6%) that were significantly effective controlling aflatoxins production on maize grains compared with control. While, the propolis and beewax found to be the most active to protect maize grains against fungi. Natural substances, carnation oil, lemongrass oil, beewax and propolis had higher active effect at low concentration on aflatoxin production more than salicylic acid and more safe for human used.","PeriodicalId":22746,"journal":{"name":"The Journal of Microbiology, Biotechnology and Food Sciences","volume":"106 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90806889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The host E. coli and its phage were isolated from sewage water. The phage infected host, E coli cells were encapsulated in alginate at specific time interval of co-cultivation, beads were stored at different temperatures. Beads were solublised to release phages and stability of released phages was evaluated by performing plaque assay at monthly interval. The number of plaque forming units increased with time of co-cultivation and by 45mins of phage-host co-cultivation, count raised from 106 to 108 pfu/ml. The infected host cells were entrapped in calcium alginate at a stage when multiple copies of mature phage are ready within host cell, and well before commencement of cell lysis.
{"title":"A NEW SIMPLE METHOD TO PRESERVE PHAGE - PRELIMINARY STUDY PRELIMINARY STUDY","authors":"A. Jape","doi":"10.15414/JMBFS.1710","DOIUrl":"https://doi.org/10.15414/JMBFS.1710","url":null,"abstract":"The host E. coli and its phage were isolated from sewage water. The phage infected host, E coli cells were encapsulated in alginate at specific time interval of co-cultivation, beads were stored at different temperatures. Beads were solublised to release phages and stability of released phages was evaluated by performing plaque assay at monthly interval. The number of plaque forming units increased with time of co-cultivation and by 45mins of phage-host co-cultivation, count raised from 106 to 108 pfu/ml. The infected host cells were entrapped in calcium alginate at a stage when multiple copies of mature phage are ready within host cell, and well before commencement of cell lysis.","PeriodicalId":22746,"journal":{"name":"The Journal of Microbiology, Biotechnology and Food Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82294785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Yassein, A. Hassan, Ebtsam Abdel-Alah, Shokri Salim
Calendula officinalis L. (pot marigold) is one of the main aromatic and medicinal plants with many uses in food and medicines. This study was carried out to demonstrate the efficiency of six colchicine concentrations (0.025, 0.05, 0.1, 0.2, 0.4 and 0.8 per cent, w/v) for Calendula improvement and induction of genetic variation. Colchicine treatments had a positive effect on the number of branches/plant, number of inflorescences, fresh and dry weight of inflorescences, inflorescence diameter, total soluble carbohydrates and β-carotene except for plant height, while seed germination and plant height were reduced. Estimation of heritability, genetic advance, genetic variability and selection of superior genotypes will be an important object in crop breeding and genetic improvement programs, and selection of genotypes with higher desirable characters. Heritability was high and ranged from 48.64 to 90.81, respectively (inflorescence diameter and plant height, respectively). Molecular markers based on a RAPD-PCR study elucidate the classification of induced Calendula mutants into two clusters. The coefficient of genetic diversity was estimated at 30%. A combination of morphological and physiological responses with molecular data contained in the various colchicine treatments illustrated the utility of RAPD-PCR as a method for identifying useful mutants and could be used to detect the colchicine effect significantly. Findings recommend the 0.05 per cent colchicine for efficient breeding calendula mutation and genetic improvement.
{"title":"MORPHOLOGICAL AND GENETIC DIVERSITY ANALYSIS IN CALENDULA (CALENDULA OFFICINALIS L.) INFLUENCED BY MUTAGENIC EFFECT OF COLCHICINE","authors":"A. Yassein, A. Hassan, Ebtsam Abdel-Alah, Shokri Salim","doi":"10.15414/JMBFS.3392","DOIUrl":"https://doi.org/10.15414/JMBFS.3392","url":null,"abstract":"Calendula officinalis L. (pot marigold) is one of the main aromatic and medicinal plants with many uses in food and medicines. This study was carried out to demonstrate the efficiency of six colchicine concentrations (0.025, 0.05, 0.1, 0.2, 0.4 and 0.8 per cent, w/v) for Calendula improvement and induction of genetic variation. Colchicine treatments had a positive effect on the number of branches/plant, number of inflorescences, fresh and dry weight of inflorescences, inflorescence diameter, total soluble carbohydrates and β-carotene except for plant height, while seed germination and plant height were reduced. Estimation of heritability, genetic advance, genetic variability and selection of superior genotypes will be an important object in crop breeding and genetic improvement programs, and selection of genotypes with higher desirable characters. Heritability was high and ranged from 48.64 to 90.81, respectively (inflorescence diameter and plant height, respectively). Molecular markers based on a RAPD-PCR study elucidate the classification of induced Calendula mutants into two clusters. The coefficient of genetic diversity was estimated at 30%. A combination of morphological and physiological responses with molecular data contained in the various colchicine treatments illustrated the utility of RAPD-PCR as a method for identifying useful mutants and could be used to detect the colchicine effect significantly. Findings recommend the 0.05 per cent colchicine for efficient breeding calendula mutation and genetic improvement.","PeriodicalId":22746,"journal":{"name":"The Journal of Microbiology, Biotechnology and Food Sciences","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74817814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-02-01DOI: 10.15414/JMBFS.2021.10.4.577-580
A. Lukin, G. Khamraeva, N. Zhuravleva
The study aims to see into the possibility of using an enzyme preparation of animal origin in canned food production technology. The technology and formula for the production of canned “Tomato and Beef Sauce” was developed. Beef of the second grade and the enzyme preparation Protepsin were used as raw materials. The effect of Protepsin on the functional and technological properties of the second grade beef was studied. The introduction of Protepsin into the meat system increased the water-binding and water-holding capacity, as well as the hydration of proteins due to the partial hydrolysis of proteins, including connective tissue, which led to an increase in functional groups and loosening of the protein structure, an increase in the immobilized moisture in meat and the penetration degree. The choice of a thickening agent in production technology for canned “Tomato and Beef Sauce” was also justified.
{"title":"RESEARCH AND DEVELOPMENT OF TECHNOLOGY FOR PRODUCTION OF CANNED FOOD USING ENZYME PREPARATION OF ANIMAL ORIGIN","authors":"A. Lukin, G. Khamraeva, N. Zhuravleva","doi":"10.15414/JMBFS.2021.10.4.577-580","DOIUrl":"https://doi.org/10.15414/JMBFS.2021.10.4.577-580","url":null,"abstract":"The study aims to see into the possibility of using an enzyme preparation of animal origin in canned food production technology. The technology and formula for the production of canned “Tomato and Beef Sauce” was developed. Beef of the second grade and the enzyme preparation Protepsin were used as raw materials. The effect of Protepsin on the functional and technological properties of the second grade beef was studied. The introduction of Protepsin into the meat system increased the water-binding and water-holding capacity, as well as the hydration of proteins due to the partial hydrolysis of proteins, including connective tissue, which led to an increase in functional groups and loosening of the protein structure, an increase in the immobilized moisture in meat and the penetration degree. The choice of a thickening agent in production technology for canned “Tomato and Beef Sauce” was also justified.","PeriodicalId":22746,"journal":{"name":"The Journal of Microbiology, Biotechnology and Food Sciences","volume":"80 1","pages":"577-580"},"PeriodicalIF":0.0,"publicationDate":"2021-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81514782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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