Pub Date : 2023-04-01DOI: 10.29261/pakvetj/2023.020
De la Cruz Montoya Aldo Hugo
Actinobacillus seminis is a Gram-negative bacterium member of the Pasteurellaceae family. Even though it is part of the ovine microbiota it is also the causal agent of genital organs affections, particularly epididymitis and orchitis. Infections caused by this microorganism generate economic losses to the ovine industry due to impaired fertility and sterility in affected animals. Knowledge about virulence factors expressed by A. seminis is scarce. In the present work, we describe the expression of a metalloprotease secreted by A. seminis that can degrade bovine fibrinogen and immunoglobulin G and present homology with a carboxy-terminal protease from A. seminis. This metalloprotease presents an optimal activity at a pH between 6 and 7, is stable up to 60°C, inactive at higher temperatures, and completely inhibited by 30 mM EDTA. The expression of this proteolytic activity is controlled by temperature, calcium, and iron. Proteases degrading extracellular matrix components and molecules involved in the immune response could facilitate and improve host colonization and invasion by A. seminis
精放线杆菌是巴氏杆菌科的革兰氏阴性菌。尽管它是绵羊微生物群的一部分，但它也是生殖器官疾病的致病因子，特别是附睾炎和睾丸炎。由于受感染动物的生育能力和不育能力受损，这种微生物引起的感染给畜牧业造成经济损失。关于半芽孢杆菌表达的毒力因子的知识很少。在本研究中，我们描述了一种金属蛋白酶的表达，这种蛋白酶可以降解牛纤维蛋白原和免疫球蛋白G，并且与一种羧基端蛋白酶具有同源性。该金属蛋白酶在pH值6 ~ 7之间表现出最佳活性，在60°C下稳定，在较高温度下失活，并被30 mM EDTA完全抑制。这种蛋白水解活性的表达受温度、钙和铁的控制。蛋白酶降解细胞外基质成分和参与免疫反应的分子，可以促进和改善半芽孢杆菌的宿主定植和入侵
{"title":"Actinobacillus seminis Secretes a Metalloprotease That Degrades Bovine Fibrinogen and IgG","authors":"De la Cruz Montoya Aldo Hugo","doi":"10.29261/pakvetj/2023.020","DOIUrl":"https://doi.org/10.29261/pakvetj/2023.020","url":null,"abstract":"Actinobacillus seminis is a Gram-negative bacterium member of the Pasteurellaceae family. Even though it is part of the ovine microbiota it is also the causal agent of genital organs affections, particularly epididymitis and orchitis. Infections caused by this microorganism generate economic losses to the ovine industry due to impaired fertility and sterility in affected animals. Knowledge about virulence factors expressed by A. seminis is scarce. In the present work, we describe the expression of a metalloprotease secreted by A. seminis that can degrade bovine fibrinogen and immunoglobulin G and present homology with a carboxy-terminal protease from A. seminis. This metalloprotease presents an optimal activity at a pH between 6 and 7, is stable up to 60°C, inactive at higher temperatures, and completely inhibited by 30 mM EDTA. The expression of this proteolytic activity is controlled by temperature, calcium, and iron. Proteases degrading extracellular matrix components and molecules involved in the immune response could facilitate and improve host colonization and invasion by A. seminis","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88572235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-01DOI: 10.29261/pakvetj/2023.012
Fatimah A Al-Saeed, S. Naz, M. Saeed, Riaz Hussain, S. Iqbal, Ahmad Manan Mustafa Chatha, Abdul Ghaffar, R. Akram
Pesticides especially insecticides are widely used in the field of agricultural development all over the world to increase crop production. Moreover, exposure to such compounds does not only influence the intended targets but also induces adverse impacts on a number of unintended targets in animals. This research aimed to determine the toxic impacts of lufenuron (an insecticide) on the health status of fish in terms of measurement of oxidative stress, antioxidant enzymes, DNA damage and histopathological changes in Nile tilapia (Oreochromis niloticus) exposed to different concentrations. For this purpose, Oreochromis niloticus fish, weighing about 75-80g were arbitrarily separated into four different groups and then exposed to lufenuron @ 0.7, 1.2 and 1.7µg/ L for 39 days. The results showed that the fish exposed to the pesticide had significant changes in oxidative stress, antioxidant profile in gills and percentile rate of DNA damage in different visceral organs including liver, kidney, and gills. Results of light microscopic investigations indicated different histological changes in liver (necrosis of hepatocytes, degeneration of hepatocytes, vacuolar degeneration and congestion), gills (necrosis of lamellar epithelial cells, telangiectasia, and atrophy of secondary lamellae), heart (congestion, necrosis of neurons, microgliosis and intracellular edema), brain (congestion, myofibrosis, neutrophilic and myocarditis) and kidneys (necrosis of renal tubules, widening of urinary space, necrosis of renal tubular epithelial cells). A significantly escalate in oxidative stress while lower quantity of antioxidant biomarkers was documented in experimental fish. The findings of this study suggest that long-term exposure to lufenuron has negative health effects via induction of DNA damage, increased oxidative stress, lowering of enzymatic antioxidants profile and histological lesions in visceral organs of Nile tilapita (Oreochromis niloticus).
{"title":"Oxidative Stress, Antioxidant Enzymes, Genotoxicity and Histopathological Profile in Oreochromis niloticus Exposed to Lufenuron","authors":"Fatimah A Al-Saeed, S. Naz, M. Saeed, Riaz Hussain, S. Iqbal, Ahmad Manan Mustafa Chatha, Abdul Ghaffar, R. Akram","doi":"10.29261/pakvetj/2023.012","DOIUrl":"https://doi.org/10.29261/pakvetj/2023.012","url":null,"abstract":"Pesticides especially insecticides are widely used in the field of agricultural development all over the world to increase crop production. Moreover, exposure to such compounds does not only influence the intended targets but also induces adverse impacts on a number of unintended targets in animals. This research aimed to determine the toxic impacts of lufenuron (an insecticide) on the health status of fish in terms of measurement of oxidative stress, antioxidant enzymes, DNA damage and histopathological changes in Nile tilapia (Oreochromis niloticus) exposed to different concentrations. For this purpose, Oreochromis niloticus fish, weighing about 75-80g were arbitrarily separated into four different groups and then exposed to lufenuron @ 0.7, 1.2 and 1.7µg/ L for 39 days. The results showed that the fish exposed to the pesticide had significant changes in oxidative stress, antioxidant profile in gills and percentile rate of DNA damage in different visceral organs including liver, kidney, and gills. Results of light microscopic investigations indicated different histological changes in liver (necrosis of hepatocytes, degeneration of hepatocytes, vacuolar degeneration and congestion), gills (necrosis of lamellar epithelial cells, telangiectasia, and atrophy of secondary lamellae), heart (congestion, necrosis of neurons, microgliosis and intracellular edema), brain (congestion, myofibrosis, neutrophilic and myocarditis) and kidneys (necrosis of renal tubules, widening of urinary space, necrosis of renal tubular epithelial cells). A significantly escalate in oxidative stress while lower quantity of antioxidant biomarkers was documented in experimental fish. The findings of this study suggest that long-term exposure to lufenuron has negative health effects via induction of DNA damage, increased oxidative stress, lowering of enzymatic antioxidants profile and histological lesions in visceral organs of Nile tilapita (Oreochromis niloticus).","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81630203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.29261/pakvetj/2023.062
{"title":"Drug Resistance and Genetic Relatedness of Escherichia coli from Mink in Northeast China","authors":"","doi":"10.29261/pakvetj/2023.062","DOIUrl":"https://doi.org/10.29261/pakvetj/2023.062","url":null,"abstract":"","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87733728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.29261/pakvetj/2023.072
{"title":"The Effect of Xylanase and Phytase Supplementation Alone or in Combination in a Wheat-based Diet on Intestinal Morphology and Blood Profile in Broilers","authors":"","doi":"10.29261/pakvetj/2023.072","DOIUrl":"https://doi.org/10.29261/pakvetj/2023.072","url":null,"abstract":"","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90091784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.29261/pakvetj/2023.074
{"title":"Protective Impacts and Involved Mechanisms of Chlorogenic Acid on Sepsis‑Associated Cognitive Deficits in Rats","authors":"","doi":"10.29261/pakvetj/2023.074","DOIUrl":"https://doi.org/10.29261/pakvetj/2023.074","url":null,"abstract":"","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"83 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84022430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.29261/pakvetj/2023.073
{"title":"Mitochondrial Dynamics Disturbances in the Liver of Broilers with Pulmonary Hypertension Syndrome","authors":"","doi":"10.29261/pakvetj/2023.073","DOIUrl":"https://doi.org/10.29261/pakvetj/2023.073","url":null,"abstract":"","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89954603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.29261/pakvetj/2023.081
{"title":"Impact of Enzymatic Hydrolyzed Protein Feeding on Rumen Microbial Population, Blood Metabolites and Performance Parameters of Lactating Dairy Cows","authors":"","doi":"10.29261/pakvetj/2023.081","DOIUrl":"https://doi.org/10.29261/pakvetj/2023.081","url":null,"abstract":"","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"216 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76593553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.29261/pakvetj/2023.060
{"title":"Annotating Susceptibility Potential of Single, Double, Tri and Tetra Mixed Infection Bacteria against Non-beta Lactam Antibiotics","authors":"","doi":"10.29261/pakvetj/2023.060","DOIUrl":"https://doi.org/10.29261/pakvetj/2023.060","url":null,"abstract":"","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80147598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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