Pub Date : 2022-10-01DOI: 10.29261/pakvetj/2022.086
Zheng Feng
Both alternative sigma factor RpoS and ubiquitous secondary messenger c-di-GMP participate in the biofilm forming of Salmonella Pullorum; however, the relationship between RpoS and c-di-GMP-regulated genes during biofilm forming remains unclear. In this study, nine genes related with c-di-GMP regulation were found to be differentially expressed (P<0.01) by RNA-seq analysis when compared with S. Pullorum strain S9 and its rpoS deletion strain S9S. Specifically, the rpoS deletion strain S9S had higher transcription level of gene STM1703 and lower c-di-GMP concentration and biofilm-forming ability than S9 (P<0.01). The STM1703 gene deletion in strains S9 and S9S significantly enhanced the c-di-GMP concentration and the biofilm-forming ability (P<0.01). qRT–PCR analysis showed that rpoS deletion or P193L substitution in RpoS increased the transcription level of the STM1703 gene by decreasing the transcription levels of the csrA and STM1344 genes (P<0.01). Overall, RpoS-mediated STM1703 negatively regulates the biofilm formation of S. Pullorum by degrading c-di-GMP
{"title":"Negative Regulation of RpoS-mediated STM1703 in Biofilm Formation of Salmonella Pullorum","authors":"Zheng Feng","doi":"10.29261/pakvetj/2022.086","DOIUrl":"https://doi.org/10.29261/pakvetj/2022.086","url":null,"abstract":"Both alternative sigma factor RpoS and ubiquitous secondary messenger c-di-GMP participate in the biofilm forming of Salmonella Pullorum; however, the relationship between RpoS and c-di-GMP-regulated genes during biofilm forming remains unclear. In this study, nine genes related with c-di-GMP regulation were found to be differentially expressed (P<0.01) by RNA-seq analysis when compared with S. Pullorum strain S9 and its rpoS deletion strain S9S. Specifically, the rpoS deletion strain S9S had higher transcription level of gene STM1703 and lower c-di-GMP concentration and biofilm-forming ability than S9 (P<0.01). The STM1703 gene deletion in strains S9 and S9S significantly enhanced the c-di-GMP concentration and the biofilm-forming ability (P<0.01). qRT–PCR analysis showed that rpoS deletion or P193L substitution in RpoS increased the transcription level of the STM1703 gene by decreasing the transcription levels of the csrA and STM1344 genes (P<0.01). Overall, RpoS-mediated STM1703 negatively regulates the biofilm formation of S. Pullorum by degrading c-di-GMP","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81822344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.29261/pakvetj/2022.081
S. Afzal
Rabies, a viral illness classified as a neglected tropical disease by the World Health Organization (WHO), causes thousands of people to die annually, primarily in underprivileged communities of Africa and Asia. In the context of a single health framework, one of the primary objectives of this literature review is to examine the major factors and challenges that maintain the endemic status of dog-mediated human rabies in Pakistan. Rabies-endemic countries have an imperative need to work on preventive strategies to lessen the burden of this terrible disease as per guidelines of World Organization for Animal Health (WOAH) and WHO. Lack of public awareness, free-roaming dogs, and cold chain mismanagement of vaccinations are the 3 most significant factors contributing to the increasing number of dog bite injuries and suspected rabies cases in animals and humans. To control dog-mediated rabies, Pakistan must initiate and strongly support a national One Health project, a subsidized supply of human and animal rabies vaccine in public hospitals, and designated rabies prevention centers at an affordable cost. People should be educated regarding responsible pet ownership and follow-up of basic preventive measures. Empowerment of labs equipped with surveillance systems are also additionally required to strengthen rabies control and prevention activities at least in regional levels. This article gives valuable information for scholars and policymakers who wish to comprehend why this disease remains endemic due to multiplex interplay of zoonotic, medical, and anthropological risk factors and transmission chains in animal and human sectors through viral reservoirs. Achieving the 'Zero By 2030' goal in Pakistan requires joint immediate action from the government, society, and all health departments at regional, provincial, and national level
{"title":"Multifactorial Complexity and Zoonotic Aspects of Dog-Mediated Human Rabies in Pakistan","authors":"S. Afzal","doi":"10.29261/pakvetj/2022.081","DOIUrl":"https://doi.org/10.29261/pakvetj/2022.081","url":null,"abstract":"Rabies, a viral illness classified as a neglected tropical disease by the World Health Organization (WHO), causes thousands of people to die annually, primarily in underprivileged communities of Africa and Asia. In the context of a single health framework, one of the primary objectives of this literature review is to examine the major factors and challenges that maintain the endemic status of dog-mediated human rabies in Pakistan. Rabies-endemic countries have an imperative need to work on preventive strategies to lessen the burden of this terrible disease as per guidelines of World Organization for Animal Health (WOAH) and WHO. Lack of public awareness, free-roaming dogs, and cold chain mismanagement of vaccinations are the 3 most significant factors contributing to the increasing number of dog bite injuries and suspected rabies cases in animals and humans. To control dog-mediated rabies, Pakistan must initiate and strongly support a national One Health project, a subsidized supply of human and animal rabies vaccine in public hospitals, and designated rabies prevention centers at an affordable cost. People should be educated regarding responsible pet ownership and follow-up of basic preventive measures. Empowerment of labs equipped with surveillance systems are also additionally required to strengthen rabies control and prevention activities at least in regional levels. This article gives valuable information for scholars and policymakers who wish to comprehend why this disease remains endemic due to multiplex interplay of zoonotic, medical, and anthropological risk factors and transmission chains in animal and human sectors through viral reservoirs. Achieving the 'Zero By 2030' goal in Pakistan requires joint immediate action from the government, society, and all health departments at regional, provincial, and national level","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89079813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.29261/pakvetj/2022.048
M. Ijaz, A. Ishtiaq, Nazia Ehsan, M. Imran, Guo-ping Zhu
Aflatoxin B1 (AFB1) is a potent mycotoxin in humans and animals. The exposure to AFB1 is evidenced to implicate multi-organ toxicity in humans and animals, particularly hepatotoxicity. Genkwanin (GNK) is a bioactive non-glycosylated flavonoid with potential pharmacological properties. Therefore, the current study aimed to determine the dose-dependent role of GNK against AFB1-instigated hepatotoxicity. The investigation was carried out on 96 adult male albino rats, which were equally distributed into eight groups. The effect of 3 different doses of GNK (5, 10 and 20 mgkg-1) was evaluated against the toxicity elicited by 50 ugkg-1 of AFB1. After the administration of AFB1 and GNK by the oral gavage for 56 days, the biochemical and hepatic serum markers were determined in addition to histopathological observation. AFB1 exposure disrupted the biochemical profile by declining the activities of antioxidant enzymes (catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase and glutathione content), while elevating the concentration of reactive oxygen species and malondialdehyde level. Furthermore, AFB1 exposure notably elevated the levels of hepatic serum enzymes (alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase) along with the levels of inflammatory markers, nuclear factor kappa-B, tumor necrosis factor-α, Interleukin-6, Interleukin-1β and activity of cyclooxygenase-2. Besides, AFB1 induction caused histopathological impairments in hepatic tissues. Nonetheless, GNK co-administration remarkably ameliorated all the damages of the hepatic system induced by AFB1 administration to the rats. Therefore, it was demonstrated that the GNK could potentially cure AFB1-instigated hepatotoxicity attributing to its antioxidative and ant-inflammatory potential
{"title":"Hepatoprotective Potential of Genkwanin Against Aflatoxin B1-Induced Biochemical, Inflammatory and Histopathological Toxicity in Rats","authors":"M. Ijaz, A. Ishtiaq, Nazia Ehsan, M. Imran, Guo-ping Zhu","doi":"10.29261/pakvetj/2022.048","DOIUrl":"https://doi.org/10.29261/pakvetj/2022.048","url":null,"abstract":"Aflatoxin B1 (AFB1) is a potent mycotoxin in humans and animals. The exposure to AFB1 is evidenced to implicate multi-organ toxicity in humans and animals, particularly hepatotoxicity. Genkwanin (GNK) is a bioactive non-glycosylated flavonoid with potential pharmacological properties. Therefore, the current study aimed to determine the dose-dependent role of GNK against AFB1-instigated hepatotoxicity. The investigation was carried out on 96 adult male albino rats, which were equally distributed into eight groups. The effect of 3 different doses of GNK (5, 10 and 20 mgkg-1) was evaluated against the toxicity elicited by 50 ugkg-1 of AFB1. After the administration of AFB1 and GNK by the oral gavage for 56 days, the biochemical and hepatic serum markers were determined in addition to histopathological observation. AFB1 exposure disrupted the biochemical profile by declining the activities of antioxidant enzymes (catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase and glutathione content), while elevating the concentration of reactive oxygen species and malondialdehyde level. Furthermore, AFB1 exposure notably elevated the levels of hepatic serum enzymes (alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase) along with the levels of inflammatory markers, nuclear factor kappa-B, tumor necrosis factor-α, Interleukin-6, Interleukin-1β and activity of cyclooxygenase-2. Besides, AFB1 induction caused histopathological impairments in hepatic tissues. Nonetheless, GNK co-administration remarkably ameliorated all the damages of the hepatic system induced by AFB1 administration to the rats. Therefore, it was demonstrated that the GNK could potentially cure AFB1-instigated hepatotoxicity attributing to its antioxidative and ant-inflammatory potential","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"66 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90673044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.29261/pakvetj/2022.087
Weiran Wang
The study investigated the bioactive ingredients and the anti-duck hepatitis A virus 1 (DHAV-1) mechanisms of Sophorae tonkinensis Radix et Rhizoma (STR) by network pharmacology (NP) and molecular docking (MD). The main bioactive ingredients of the STR were obtained using TCMSP database. Cytoscape 3.8.2 software was used for topology analysis and construction of the STR-active molecule-target interaction network. The STRING database and Cytoscape plotted Protein-protein interaction (PPI) networks. The key targets of STR were analyzed and enriched by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment. Finally, the main bioactive ingredients of the STR were verified by MD. The STR-DHAV-1 target network included 13 ingredients and 34 target genes. The key target gene is IL-6. KEGG analysis revealed that the main pathways included AGE-RAGE signaling pathway in diabetic complications, pathways in cancer, and C-type lectin receptor signaling pathway. MD results further verified that the main bioactive components identified in the STR were quercetin, kaempferol and matrine, which had higher binding activities to target. Network pharmacology and molecular docking studies revealed that quercetin, kaempferol and matrine were the main bioactive ingredients of STR and might play a crucial role in potential molecular DHAV-1 therapeutic mechanisms
采用网络药理学(NP)和分子对接(MD)技术研究了东参根瘤(STR)的生物活性成分及其抗鸭甲型肝炎病毒(DHAV-1)的作用机制。通过TCMSP数据库获得其主要生物活性成分。利用Cytoscape 3.8.2软件对str活性分子-靶点相互作用网络进行拓扑分析和构建。STRING数据库和Cytoscape绘制了蛋白质-蛋白质相互作用(PPI)网络。通过基因本体(Gene Ontology, GO)和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes, KEGG)富集对STR的关键靶点进行分析和富集。最后，通过MD对STR的主要生物活性成分进行了验证。STR- dhav -1靶点网络包括13种成分和34个靶基因。关键靶基因是IL-6。KEGG分析显示，其主要通路包括糖尿病并发症中的AGE-RAGE信号通路、癌症中的AGE-RAGE信号通路和c型凝集素受体信号通路。MD结果进一步验证了STR中鉴定的主要生物活性成分为槲皮素、山奈酚和苦参碱，它们与靶标具有较高的结合活性。网络药理学和分子对接研究发现槲皮素、山奈酚和苦参碱是STR的主要生物活性成分，可能在DHAV-1的潜在分子治疗机制中发挥重要作用
{"title":"Exploring the Molecular Mechanisms of Sophorae tonkinensis Radix et Rhizoma anti-DHAV-1 by Network Pharmacology Analysis","authors":"Weiran Wang","doi":"10.29261/pakvetj/2022.087","DOIUrl":"https://doi.org/10.29261/pakvetj/2022.087","url":null,"abstract":"The study investigated the bioactive ingredients and the anti-duck hepatitis A virus 1 (DHAV-1) mechanisms of Sophorae tonkinensis Radix et Rhizoma (STR) by network pharmacology (NP) and molecular docking (MD). The main bioactive ingredients of the STR were obtained using TCMSP database. Cytoscape 3.8.2 software was used for topology analysis and construction of the STR-active molecule-target interaction network. The STRING database and Cytoscape plotted Protein-protein interaction (PPI) networks. The key targets of STR were analyzed and enriched by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment. Finally, the main bioactive ingredients of the STR were verified by MD. The STR-DHAV-1 target network included 13 ingredients and 34 target genes. The key target gene is IL-6. KEGG analysis revealed that the main pathways included AGE-RAGE signaling pathway in diabetic complications, pathways in cancer, and C-type lectin receptor signaling pathway. MD results further verified that the main bioactive components identified in the STR were quercetin, kaempferol and matrine, which had higher binding activities to target. Network pharmacology and molecular docking studies revealed that quercetin, kaempferol and matrine were the main bioactive ingredients of STR and might play a crucial role in potential molecular DHAV-1 therapeutic mechanisms","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82670912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.29261/pakvetj/2022.071
F. Nworah
Snail slime is used widely in African traditional medicine, specifically in Nsukka Enugu, Nigeria, for wound management; however, this claim lacks scientific validation. Herein, we investigated the gastroprotective effect of aqueous Achatina achatina L. (snail) slime extract (ASSE) on indomethacin- and ethanol-induced ulceration in Wistar albino rat models. Biochemical analysis of ASSE showed appreciable levels of manganese, iron, zinc, copper, molybdenum, selenium, Vitamin C, B1, B2, and B3 and a basic pH. The experimental design consists of two Phases (five groups of five rats each). In both Phases, group I (positive control) received 3ml of distilled water, while groups II-V received 20 mg/kg body weight omeprazole (standard drug), 50, 100, and 200 mg/kg bw ASSE, respectively, via oral intubation. The various treatments lasted for 21-days. Following 24-hours fasting with access to only water (which spanned between the 22nd -23rd day), ulceration was induced separately on the experimental animals in Phase I with a single oral dose of indomethacin (30 mg/kg bw) and in Phase II with a single oral dose of acidified ethanol (1.5 ml/ kg bw), via the gastric gavage. The LD50 study showed safety up to 5000 mg/kg bw ASSE. After induction, a significant (P<0.05) increase in the number of ulcers and mean ulcer index were recorded in group 1 of both Phases; however, rats administered various concentrations of ASSE showed significant (P<0.05) amelioration of the ulceration in both Phases, and these were on par with the standard control. These results suggest that aqueous snail slime extract possesses gastroprotective potential
{"title":"Gastroprotective Effect of Aqueous Achatina achatina L. (Snail) Slime Extract on Indomethacin- and Acidified Ethanol-Induced Ulceration in Wistar Albino Rats","authors":"F. Nworah","doi":"10.29261/pakvetj/2022.071","DOIUrl":"https://doi.org/10.29261/pakvetj/2022.071","url":null,"abstract":"Snail slime is used widely in African traditional medicine, specifically in Nsukka Enugu, Nigeria, for wound management; however, this claim lacks scientific validation. Herein, we investigated the gastroprotective effect of aqueous Achatina achatina L. (snail) slime extract (ASSE) on indomethacin- and ethanol-induced ulceration in Wistar albino rat models. Biochemical analysis of ASSE showed appreciable levels of manganese, iron, zinc, copper, molybdenum, selenium, Vitamin C, B1, B2, and B3 and a basic pH. The experimental design consists of two Phases (five groups of five rats each). In both Phases, group I (positive control) received 3ml of distilled water, while groups II-V received 20 mg/kg body weight omeprazole (standard drug), 50, 100, and 200 mg/kg bw ASSE, respectively, via oral intubation. The various treatments lasted for 21-days. Following 24-hours fasting with access to only water (which spanned between the 22nd -23rd day), ulceration was induced separately on the experimental animals in Phase I with a single oral dose of indomethacin (30 mg/kg bw) and in Phase II with a single oral dose of acidified ethanol (1.5 ml/ kg bw), via the gastric gavage. The LD50 study showed safety up to 5000 mg/kg bw ASSE. After induction, a significant (P<0.05) increase in the number of ulcers and mean ulcer index were recorded in group 1 of both Phases; however, rats administered various concentrations of ASSE showed significant (P<0.05) amelioration of the ulceration in both Phases, and these were on par with the standard control. These results suggest that aqueous snail slime extract possesses gastroprotective potential","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80896166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.29261/pakvetj/2022.060
Xiu P. Chen
Intestinal parasites are of great economic importance in livestock. However, scarcity of data has been found about the prevalence of four important intestinal parasites including Cryptosporidium sp., Giardia duodenalis, Enterocytozoon bieneusi and Blastocystis sp. infection in free-ranged yaks and Tibetan pigs during the winter season on the plateau. Fecal samples of yaks (n=40) and Tibetan pigs (n=60) were collected and molecular identification of these parasites was performed through nested PCR amplification. Positive PCR samples were sequenced and further phylogenetic analysis was performed. Results found that the prevalence of Cryptosporidium sp., G. duodenalis, E. bieneusi and Blastocystis sp. was 10.0, 7.5, 12.5, 7.5% in yaks, and 18.3, 0, 56.7, 50.0% in Tibetan pigs, respectively. Co-infections was found between 2.5-7.5% in yaks and 0-26.7% in Tibetan pigs, respectively. The sequenced samples were identified to be Cryptosporidium sp. and was identified as pig genotype II. Findings of this study will provide an insight to the prevention and control for these important parasites on the high plateau
{"title":"Molecular Epidemiological Investigation of Cryptosporidium sp., Giardia duodenalis, Enterocytozoon bieneusi and Blastocystis sp. Infection in Free-ranged Yaks and Tibetan Pigs on the Plateau","authors":"Xiu P. Chen","doi":"10.29261/pakvetj/2022.060","DOIUrl":"https://doi.org/10.29261/pakvetj/2022.060","url":null,"abstract":"Intestinal parasites are of great economic importance in livestock. However, scarcity of data has been found about the prevalence of four important intestinal parasites including Cryptosporidium sp., Giardia duodenalis, Enterocytozoon bieneusi and Blastocystis sp. infection in free-ranged yaks and Tibetan pigs during the winter season on the plateau. Fecal samples of yaks (n=40) and Tibetan pigs (n=60) were collected and molecular identification of these parasites was performed through nested PCR amplification. Positive PCR samples were sequenced and further phylogenetic analysis was performed. Results found that the prevalence of Cryptosporidium sp., G. duodenalis, E. bieneusi and Blastocystis sp. was 10.0, 7.5, 12.5, 7.5% in yaks, and 18.3, 0, 56.7, 50.0% in Tibetan pigs, respectively. Co-infections was found between 2.5-7.5% in yaks and 0-26.7% in Tibetan pigs, respectively. The sequenced samples were identified to be Cryptosporidium sp. and was identified as pig genotype II. Findings of this study will provide an insight to the prevention and control for these important parasites on the high plateau","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79957269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.29261/pakvetj/2022.069
H. Mustafa
The leading factor in tooth loss, periodontitis is a global public health concern. Common periodontal treatments include medication and mechanical therapy. Regarding the use of natural medicines to treat periodontitis, there has been much advancement. Cell proliferation, migration, differentiation, and homeostasis are modulated by tight junction (TJs). The present study reports the effectiveness of egg yolk oil (EYO) as a treatment for ligature-induced periodontitis by analyzing its microstructure and its effect on claudin-1. Eighteen mature male and female rats were used in the investigation, and they were categorized: Group 1 (Control Negative, n=9), which received no treatment; Group 2 (Control Positive, n=9), which received a silk ligature to cause periodontitis. The EYO treatment+ periodontitis for rats in Group 3 (EYO, n=9) was applied by ligature for 30 days. The incisor teeth with periodontal tissues were collected on days 7, 14, and 30 of the experiment and sections were prepared for both H&E staining and IHC for claudin-1 gene utilization. There was a significant improvement in alveolar bone loss, reduction in the inflammatory reaction, regeneration of PDL thickness, reduction of osteoclast numbers, and activation of claudin-1 tight junction expression compared to the control group in the entire experiment period. Our research concluded that EYO may have a critical role in alveolar bone formation with the regeneration of periodontal tissue damage that is associated with ligature-induced periodontitis via up-regulation of the expression of claudin-1 tight junction proteins that provide regeneration and repair processes, hence reducing pathological damages
{"title":"The Effect of Egg Yolk Oil in Repairing Tight Junction Claudin-1 in Periodontitis in a Wistar Rat","authors":"H. Mustafa","doi":"10.29261/pakvetj/2022.069","DOIUrl":"https://doi.org/10.29261/pakvetj/2022.069","url":null,"abstract":"The leading factor in tooth loss, periodontitis is a global public health concern. Common periodontal treatments include medication and mechanical therapy. Regarding the use of natural medicines to treat periodontitis, there has been much advancement. Cell proliferation, migration, differentiation, and homeostasis are modulated by tight junction (TJs). The present study reports the effectiveness of egg yolk oil (EYO) as a treatment for ligature-induced periodontitis by analyzing its microstructure and its effect on claudin-1. Eighteen mature male and female rats were used in the investigation, and they were categorized: Group 1 (Control Negative, n=9), which received no treatment; Group 2 (Control Positive, n=9), which received a silk ligature to cause periodontitis. The EYO treatment+ periodontitis for rats in Group 3 (EYO, n=9) was applied by ligature for 30 days. The incisor teeth with periodontal tissues were collected on days 7, 14, and 30 of the experiment and sections were prepared for both H&E staining and IHC for claudin-1 gene utilization. There was a significant improvement in alveolar bone loss, reduction in the inflammatory reaction, regeneration of PDL thickness, reduction of osteoclast numbers, and activation of claudin-1 tight junction expression compared to the control group in the entire experiment period. Our research concluded that EYO may have a critical role in alveolar bone formation with the regeneration of periodontal tissue damage that is associated with ligature-induced periodontitis via up-regulation of the expression of claudin-1 tight junction proteins that provide regeneration and repair processes, hence reducing pathological damages","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83391697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.29261/pakvetj/2022.066
Kubilay Dogan Kilic
Gestational and lactational transmission of Aflatoxin B1 (AFB1) can elicit several toxic effects emphasizing the severity of aflatoxicosis. The present study aimed to investigate the genotoxic effects of prenatal and postnatal exposure to AFB1 on the livers of exposed offspring. With this aim, 50 μg/kg/body weight per day AFB1 was administered intraperitoneally (i.p.) to pregnant and lactating dam rats. Pups grouped as newborns (GD21/PND0) exposed in utero and infants exposed through breast milk (PND21) were compared with body weight measurements. Liver tissues were weighed after removal and subjected to histochemical (HC), immunohistochemical (IHC) and biochemical analyzes. The body weight and liver weight of exposed newborns were significantly lower than control (P<0.05). The histomorphological changes were more pronounced in exposed newborns. A decrease (P<0.05) in the histological score (HSCORE) of cytokeratin 19 (CK19) IHC, fetal stem/progenitor cells marker, and an increase (P<0.05) in the HSCORE of alpha-fetoprotein (AFP) IHC, hepatocellular carcinoma (HCC) marker, were detected in both exposed groups. Exposed newborns showed higher CK19 and AFP HSCORE than exposed infants (P<0.05). Both groups exhibited a low proliferation index score of proliferating cell nuclear antigen (PCNA) IHC (P<0.05). The high apoptotic index score of immunofluorescence (IF) staining of the terminal deoxytransferase-mediated dUTP nick-end labeling (TUNEL) method was significant in exposed newborns (P<0.05). Evaluation of oxidative stress and antioxidant systems revealed that tissue malondialdehyde (MDA) levels decreased in exposed newborns and increased in exposed infants (P<0.05), and tissue catalase (CAT) levels increased in both groups (P<0.05). In conclusion, the effects of AFB1 exposure during the gestational period occurred more severely, and the importance of preventing AFB1 exposure was revealed
{"title":"Liver Histology and Biochemistry of Exposed Newborn and Infant Rats with Experimental Aflatoxicosis","authors":"Kubilay Dogan Kilic","doi":"10.29261/pakvetj/2022.066","DOIUrl":"https://doi.org/10.29261/pakvetj/2022.066","url":null,"abstract":"Gestational and lactational transmission of Aflatoxin B1 (AFB1) can elicit several toxic effects emphasizing the severity of aflatoxicosis. The present study aimed to investigate the genotoxic effects of prenatal and postnatal exposure to AFB1 on the livers of exposed offspring. With this aim, 50 μg/kg/body weight per day AFB1 was administered intraperitoneally (i.p.) to pregnant and lactating dam rats. Pups grouped as newborns (GD21/PND0) exposed in utero and infants exposed through breast milk (PND21) were compared with body weight measurements. Liver tissues were weighed after removal and subjected to histochemical (HC), immunohistochemical (IHC) and biochemical analyzes. The body weight and liver weight of exposed newborns were significantly lower than control (P<0.05). The histomorphological changes were more pronounced in exposed newborns. A decrease (P<0.05) in the histological score (HSCORE) of cytokeratin 19 (CK19) IHC, fetal stem/progenitor cells marker, and an increase (P<0.05) in the HSCORE of alpha-fetoprotein (AFP) IHC, hepatocellular carcinoma (HCC) marker, were detected in both exposed groups. Exposed newborns showed higher CK19 and AFP HSCORE than exposed infants (P<0.05). Both groups exhibited a low proliferation index score of proliferating cell nuclear antigen (PCNA) IHC (P<0.05). The high apoptotic index score of immunofluorescence (IF) staining of the terminal deoxytransferase-mediated dUTP nick-end labeling (TUNEL) method was significant in exposed newborns (P<0.05). Evaluation of oxidative stress and antioxidant systems revealed that tissue malondialdehyde (MDA) levels decreased in exposed newborns and increased in exposed infants (P<0.05), and tissue catalase (CAT) levels increased in both groups (P<0.05). In conclusion, the effects of AFB1 exposure during the gestational period occurred more severely, and the importance of preventing AFB1 exposure was revealed","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83538753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.29261/pakvetj/2022.026
Kainat Ishaq
The present study aimed to evaluate the prevalence of Proteus mirabilis (P. mirabilis) in poultry meat and to find its antimicrobial susceptibility. P. mirabilis has been frequently isolated from poultry and poses potential threat to public health. The pathogen resides in broiler’s intestine, so it can be a source of contamination of chicken carcass in a slaughterhouse. A total of 50 samples of chicken carcass (from liver n=15, from intestine n=15, from thigh n=11 and from wings n=9) were taken from Faisalabad and cultured on Xylose Lysine Deoxycholate agar (XLD) for isolation and purification of P. mirabilis. Percent positivity of P. mirabilis in liver was found to be 60%, 46% in intestine, 36% in thigh and 33% in wings. P. mirabilis isolates showed high resistance to tetracycline (100%) and piperacillin (91.3%) while low resistance was shown to ceftazidime (8.6%). The study concluded that chicken could be the source of P. mirabilis and it can be a source of infection in human and animals.
{"title":"Occurrence and Antimicrobial Susceptibility of Proteus mirabilis from Chicken Carcass","authors":"Kainat Ishaq","doi":"10.29261/pakvetj/2022.026","DOIUrl":"https://doi.org/10.29261/pakvetj/2022.026","url":null,"abstract":"The present study aimed to evaluate the prevalence of Proteus mirabilis (P. mirabilis) in poultry meat and to find its antimicrobial susceptibility. P. mirabilis has been frequently isolated from poultry and poses potential threat to public health. The pathogen resides in broiler’s intestine, so it can be a source of contamination of chicken carcass in a slaughterhouse. A total of 50 samples of chicken carcass (from liver n=15, from intestine n=15, from thigh n=11 and from wings n=9) were taken from Faisalabad and cultured on Xylose Lysine Deoxycholate agar (XLD) for isolation and purification of P. mirabilis. Percent positivity of P. mirabilis in liver was found to be 60%, 46% in intestine, 36% in thigh and 33% in wings. P. mirabilis isolates showed high resistance to tetracycline (100%) and piperacillin (91.3%) while low resistance was shown to ceftazidime (8.6%). The study concluded that chicken could be the source of P. mirabilis and it can be a source of infection in human and animals.","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73490912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.29261/pakvetj/2022.025
Haytham Mohamedelfatih Mohamed Makki
Gibberellic acid (GBA) is a natural plant growth hormone, controlling many developmental processes. In order to explore the protective effects of GBA against Thioacetamide (TAA)-induced acute liver toxicity, thirty male Sprague-Dawley (SD) rats were randomly allocated into six groups (5 rats/group); Normal control (NC): received distilled water (DW) per os (p.o.), GBA only: received (20 mg/kg, p.o.) of GBA, TAA: received DW and TAA (350 mg/kg, i.p.), GBA 5: received GBA (5 mg/kg, p.o.) and TAA (350 mg/kg, i.p.), GBA 10: received GBA (10 mg/kg, p.o.) and TAA (350 mg/kg, i.p.), and GBA 20: received GBA (20 mg/kg, p.o.) and TAA (350 mg/kg, i.p.). Blood and plasma were collected for biochemical analysis while liver tissues were harvested and preserved for histological analysis. The results confirmed the TAA-induced acute liver toxicity by the significantly increased liver enzymes, hypoglycemia, hypoxia, polycythemia, and metabolic acidosis as compared with NC. However, pretreatment of TAA-intoxicated rats with GBA indicated the hepatoprotective effects by reducing liver enzyme levels significantly and alleviating hepatic lesions in a dose-dependent manner
{"title":"Effects of Gibberellic Acid on Thioacetamide-Induced Acute Liver Toxicity in Sprague-Dawley Rats","authors":"Haytham Mohamedelfatih Mohamed Makki","doi":"10.29261/pakvetj/2022.025","DOIUrl":"https://doi.org/10.29261/pakvetj/2022.025","url":null,"abstract":"Gibberellic acid (GBA) is a natural plant growth hormone, controlling many developmental processes. In order to explore the protective effects of GBA against Thioacetamide (TAA)-induced acute liver toxicity, thirty male Sprague-Dawley (SD) rats were randomly allocated into six groups (5 rats/group); Normal control (NC): received distilled water (DW) per os (p.o.), GBA only: received (20 mg/kg, p.o.) of GBA, TAA: received DW and TAA (350 mg/kg, i.p.), GBA 5: received GBA (5 mg/kg, p.o.) and TAA (350 mg/kg, i.p.), GBA 10: received GBA (10 mg/kg, p.o.) and TAA (350 mg/kg, i.p.), and GBA 20: received GBA (20 mg/kg, p.o.) and TAA (350 mg/kg, i.p.). Blood and plasma were collected for biochemical analysis while liver tissues were harvested and preserved for histological analysis. The results confirmed the TAA-induced acute liver toxicity by the significantly increased liver enzymes, hypoglycemia, hypoxia, polycythemia, and metabolic acidosis as compared with NC. However, pretreatment of TAA-intoxicated rats with GBA indicated the hepatoprotective effects by reducing liver enzyme levels significantly and alleviating hepatic lesions in a dose-dependent manner","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75453863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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