Pub Date : 2019-01-01DOI: 10.32268/JPROTOZOOLRES.29.1-2_44
Kalyetsi Rogers, N. Gertrude, M. Enoch
{"title":"Malaria infections among pregnant women attending antenatal clinic at Bududa hospital, eastern Uganda","authors":"Kalyetsi Rogers, N. Gertrude, M. Enoch","doi":"10.32268/JPROTOZOOLRES.29.1-2_44","DOIUrl":"https://doi.org/10.32268/JPROTOZOOLRES.29.1-2_44","url":null,"abstract":"","PeriodicalId":22861,"journal":{"name":"The Journal of protozoology research","volume":"23 1","pages":"44-50"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79487920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.32268/JPROTOZOOLRES.29.1-2_26
T. Munkhjargal, G. Batiha, A. M. Beshbishy, M. Rizk, Azirwan Guswanto, T. Onikubo, T. Dickson, N. Yokoyama, I. Igarashi
{"title":"Improvement of SYBR Green I−based fluorescence assay reading procedure for anti−babesial drugs screening in vitro","authors":"T. Munkhjargal, G. Batiha, A. M. Beshbishy, M. Rizk, Azirwan Guswanto, T. Onikubo, T. Dickson, N. Yokoyama, I. Igarashi","doi":"10.32268/JPROTOZOOLRES.29.1-2_26","DOIUrl":"https://doi.org/10.32268/JPROTOZOOLRES.29.1-2_26","url":null,"abstract":"","PeriodicalId":22861,"journal":{"name":"The Journal of protozoology research","volume":"30 1","pages":"26-43"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75858627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.32268/JPROTOZOOLRES.29.1-2_1
A. Ybañez, R. H. Ybañez, A. Estrera, Maxfrancis G. Talle, Mingming Liu, X. Xuan
{"title":"Detection of Mycoplasma and Hepatozoon spp. in Philippine Dogs","authors":"A. Ybañez, R. H. Ybañez, A. Estrera, Maxfrancis G. Talle, Mingming Liu, X. Xuan","doi":"10.32268/JPROTOZOOLRES.29.1-2_1","DOIUrl":"https://doi.org/10.32268/JPROTOZOOLRES.29.1-2_1","url":null,"abstract":"","PeriodicalId":22861,"journal":{"name":"The Journal of protozoology research","volume":"20 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89508823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.32268/JPROTOZOOLRES.29.1-2_8
G. Batiha, A. M. Beshbishy, D. S. Tayebwa, O. Adeyemi, N. Yokoyama, I. Igarashi
The available drugs against piroplasmosis are insufficient and faced with several challenges, such as drug-resistant parasites and toxicity to treated animals. Therefore, the discovery of new drug compounds is necessary for the effective control of babesiosis and theileriosis. Methanolic Peganum harmala seed extract (MPHSE) and ethanolic Artemisia absinthium leaf extract (EAALE) have several medicinal properties. In the present study, the growth-inhibition effects of MPHSE and EAALE were evaluated in vitro and in vivo. The half-maximal inhibitory concentration (IC50) values for MPHSE against Babesia bovis, B. bigemina, B. divergens, B. caballi, and Theileria equi were 24.9 ± 1.2, 77.1 ± 2.3, 61.1 ± 2.9, 80.8 ± 4.1, and 11.3 ± 2.1 μg/mL, respectively. EAALE exhibited IC50 values of 43.3 ± 3.1, 39.2 ± 2.7, 38.5 ±3.7, 50.3 ± 2.1, and 28.2 ± 2.1 μg/mL against B. bovis, B. bigemina, B. divergens, B. caballi, and T. equi, respectively. The toxicity assay on Madin–Darby bovine kidney (MDBK), mouse embryonic fibroblast (NIH/3T3), and human foreskin fibroblast (HFF) cell lines showed that MPHSE affected the viability of MDBK, NIH/3T3, and HFF cell lines with half maximum effective concentration (EC50) values of 611.7± 10.9, 870 ± 22, and ˃1500 μg/mL, respectively, while EAALE exhibited EC50 values of 340.7 ± 8.5, 736.7 ± 9.3, and 1371.5 ± 17.3 μg/mL against MDBK, NIH/3T3, and HFF cell lines, respectively. In the in vivo experiment, MPHSE and EAALE oral treatments at 150 mg/kg inhibited the growth of Babesia microti in mice by 60% and 55.1%, respectively. These findings suggest that MPHSE and EAALE have the potential to be alternative remedies for treating piroplasmosis.
{"title":"Anti-piroplasmic potential of the methanolic Peganum harmala seeds and ethanolic Artemisia absinthium leaf extracts","authors":"G. Batiha, A. M. Beshbishy, D. S. Tayebwa, O. Adeyemi, N. Yokoyama, I. Igarashi","doi":"10.32268/JPROTOZOOLRES.29.1-2_8","DOIUrl":"https://doi.org/10.32268/JPROTOZOOLRES.29.1-2_8","url":null,"abstract":"The available drugs against piroplasmosis are insufficient and faced with several challenges, such as drug-resistant parasites and toxicity to treated animals. Therefore, the discovery of new drug compounds is necessary for the effective control of babesiosis and theileriosis. Methanolic Peganum harmala seed extract (MPHSE) and ethanolic Artemisia absinthium leaf extract (EAALE) have several medicinal properties. In the present study, the growth-inhibition effects of MPHSE and EAALE were evaluated in vitro and in vivo. The half-maximal inhibitory concentration (IC50) values for MPHSE against Babesia bovis, B. bigemina, B. divergens, B. caballi, and Theileria equi were 24.9 ± 1.2, 77.1 ± 2.3, 61.1 ± 2.9, 80.8 ± 4.1, and 11.3 ± 2.1 μg/mL, respectively. EAALE exhibited IC50 values of 43.3 ± 3.1, 39.2 ± 2.7, 38.5 ±3.7, 50.3 ± 2.1, and 28.2 ± 2.1 μg/mL against B. bovis, B. bigemina, B. divergens, B. caballi, and T. equi, respectively. The toxicity assay on Madin–Darby bovine kidney (MDBK), mouse embryonic fibroblast (NIH/3T3), and human foreskin fibroblast (HFF) cell lines showed that MPHSE affected the viability of MDBK, NIH/3T3, and HFF cell lines with half maximum effective concentration (EC50) values of 611.7± 10.9, 870 ± 22, and ˃1500 μg/mL, respectively, while EAALE exhibited EC50 values of 340.7 ± 8.5, 736.7 ± 9.3, and 1371.5 ± 17.3 μg/mL against MDBK, NIH/3T3, and HFF cell lines, respectively. In the in vivo experiment, MPHSE and EAALE oral treatments at 150 mg/kg inhibited the growth of Babesia microti in mice by 60% and 55.1%, respectively. These findings suggest that MPHSE and EAALE have the potential to be alternative remedies for treating piroplasmosis.","PeriodicalId":22861,"journal":{"name":"The Journal of protozoology research","volume":"116 1","pages":"8-25"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73136079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.32268/JPROTOZOOLRES.29.1-2_51
Yoichiro Sogame, R. Saito, T. Sakai, Taiga Shimizu, Taiki Ono, R. Koizumi, Kaito Mizumachi
{"title":"Lepidosomes acquire fluorescence after encystation: Including additional notes of morphological events during encystation and reconsideration of the morphological features in the ciliate Colpoda cucullus","authors":"Yoichiro Sogame, R. Saito, T. Sakai, Taiga Shimizu, Taiki Ono, R. Koizumi, Kaito Mizumachi","doi":"10.32268/JPROTOZOOLRES.29.1-2_51","DOIUrl":"https://doi.org/10.32268/JPROTOZOOLRES.29.1-2_51","url":null,"abstract":"","PeriodicalId":22861,"journal":{"name":"The Journal of protozoology research","volume":"58 1","pages":"51-62"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76201802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.32268/JPROTOZOOLRES.28.1-2_1
Seyed Sajad Shahrokhi, M. Tabandeh, M. Kesmati
{"title":"The effects of magnesium oxide nanoparticles on population growth of Paramecium caudatum.","authors":"Seyed Sajad Shahrokhi, M. Tabandeh, M. Kesmati","doi":"10.32268/JPROTOZOOLRES.28.1-2_1","DOIUrl":"https://doi.org/10.32268/JPROTOZOOLRES.28.1-2_1","url":null,"abstract":"","PeriodicalId":22861,"journal":{"name":"The Journal of protozoology research","volume":"438 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78229758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-01DOI: 10.32268/JPROTOZOOLRES.21.1_20
L. Wangai, S. Omar
Development and spread of chloroquine (CQ) resistance led to its withdrawal in most malaria endemic countries. In Kenya, this occurred in 1998 when clinical efficacy dropped below 50%. Less than a decade after CQ was removed from routine use in Malawi, the drug has reversed to activity and is again effective for first-line treatment of uncomplicated malaria. There is a probability of a similar reversed activity in Kenya for more 10 years of its absence in uncomplicated Plasmodium falciparum malaria treatment. The present study was aimed at establishing the CQ resistance status in the country, 10 years after its withdrawal, by looking at high malaria transmission zone, Mbita, a malaria endemic area and some malaria epidemic areas of the Kenyan highlands. The prevalence of T76 and Y86 P. falciparum molecular markers for CQ resistance in Pfcrt and Pfmdr1 genes were investigated by PCR-RFLP and dot blot analysis in 64 samples collected in March to May 2007 in the endemic area and 38 samples collected in April to July the same year in the epidemics. The study shows that 67.3% of field isolates from the endemic site still harbor Y86 mutation in Pfmdr1 while 32.7% have the wild type allele N86 compared to the 94% and 6 % prevalence observed in Mwea, an endemic area, in 2004 (χ 2 =10.08, P=0.00015, 95% CI=2.085-27.8). In the epidemics 75% of field isolates from the epidemic sites still harbor Y86 mutation in Pfmdr1 while 25% have the wild type allele N86 compared to the 91.6% and 8.4% prevalence observed in an epidemic area in 1997 (χ 2 =1.585, P=0.208, 95% CI=0.701-19.176). From the study there is a significant change in the proportions of the resistant genotypes in the endemic areas while in the epidemics, there was also a noticeable shift though not significant. This therefore indicates a slow but steady re-emergence of P. falciparum CQ sensitive strains in the country. Though does not warrant the reintroduction of CQ for malaria treatment.
{"title":"Chloroquine resistance status a decade after: Re-emergence of sensitive Plasmodium falciparum strains in malaria endemic and epidemic areas in Kenya","authors":"L. Wangai, S. Omar","doi":"10.32268/JPROTOZOOLRES.21.1_20","DOIUrl":"https://doi.org/10.32268/JPROTOZOOLRES.21.1_20","url":null,"abstract":"Development and spread of chloroquine (CQ) resistance led to its withdrawal in most malaria endemic countries. In Kenya, this occurred in 1998 when clinical efficacy dropped below 50%. Less than a decade after CQ was removed from routine use in Malawi, the drug has reversed to activity and is again effective for first-line treatment of uncomplicated malaria. There is a probability of a similar reversed activity in Kenya for more 10 years of its absence in uncomplicated Plasmodium falciparum malaria treatment. The present study was aimed at establishing the CQ resistance status in the country, 10 years after its withdrawal, by looking at high malaria transmission zone, Mbita, a malaria endemic area and some malaria epidemic areas of the Kenyan highlands. The prevalence of T76 and Y86 P. falciparum molecular markers for CQ resistance in Pfcrt and Pfmdr1 genes were investigated by PCR-RFLP and dot blot analysis in 64 samples collected in March to May 2007 in the endemic area and 38 samples collected in April to July the same year in the epidemics. The study shows that 67.3% of field isolates from the endemic site still harbor Y86 mutation in Pfmdr1 while 32.7% have the wild type allele N86 compared to the 94% and 6 % prevalence observed in Mwea, an endemic area, in 2004 (χ 2 =10.08, P=0.00015, 95% CI=2.085-27.8). In the epidemics 75% of field isolates from the epidemic sites still harbor Y86 mutation in Pfmdr1 while 25% have the wild type allele N86 compared to the 91.6% and 8.4% prevalence observed in an epidemic area in 1997 (χ 2 =1.585, P=0.208, 95% CI=0.701-19.176). From the study there is a significant change in the proportions of the resistant genotypes in the endemic areas while in the epidemics, there was also a noticeable shift though not significant. This therefore indicates a slow but steady re-emergence of P. falciparum CQ sensitive strains in the country. Though does not warrant the reintroduction of CQ for malaria treatment.","PeriodicalId":22861,"journal":{"name":"The Journal of protozoology research","volume":"28 1","pages":"20-29"},"PeriodicalIF":0.0,"publicationDate":"2011-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87976529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-01-01DOI: 10.32268/JPROTOZOOLRES.20.1_45
B. Shamaki, B. M. Agaie, P. O. Ajagbonna, A. Elsa, Y. A. Bashir, B. Iliyasu, A. A. Ebbo, A. Shehu, A. Sakuma
{"title":"In vitro studies on the anti-trypanosomal effect of Jatropha gossypiifolia var gossypiifolia","authors":"B. Shamaki, B. M. Agaie, P. O. Ajagbonna, A. Elsa, Y. A. Bashir, B. Iliyasu, A. A. Ebbo, A. Shehu, A. Sakuma","doi":"10.32268/JPROTOZOOLRES.20.1_45","DOIUrl":"https://doi.org/10.32268/JPROTOZOOLRES.20.1_45","url":null,"abstract":"","PeriodicalId":22861,"journal":{"name":"The Journal of protozoology research","volume":"12 1","pages":"45-50"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75345167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-01-01DOI: 10.32268/JPROTOZOOLRES.20.2_20
N. Yokoyama, Y. Ikehara, W. Tiwananthagorn, N. Ota, I. Igarashi, N. Kojima, O. Taguchi, K. Tsujimura
{"title":"Differences in CD4+CD25+ regulatory T cell-depletion between Babesia microti and Babesia rodhaini infections in mice","authors":"N. Yokoyama, Y. Ikehara, W. Tiwananthagorn, N. Ota, I. Igarashi, N. Kojima, O. Taguchi, K. Tsujimura","doi":"10.32268/JPROTOZOOLRES.20.2_20","DOIUrl":"https://doi.org/10.32268/JPROTOZOOLRES.20.2_20","url":null,"abstract":"","PeriodicalId":22861,"journal":{"name":"The Journal of protozoology research","volume":"5 1","pages":"20-30"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78858122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-01-01DOI: 10.32268/JPROTOZOOLRES.20.1_59
G. Aboge, V. Batbaatar, Y. Goo, J. Yamagishi, Y. Nishikawa, F. Sunaga, K. Namikawa, I. Igarashi, K. Fujisaki, Hiroshi Suzuki, X. Xuan
{"title":"Molecular characterization and expression of a 47- kDa merozoite surface protein of Babesia gibsoni for serodiagnosis by enzyme-linked immunosorbent assay","authors":"G. Aboge, V. Batbaatar, Y. Goo, J. Yamagishi, Y. Nishikawa, F. Sunaga, K. Namikawa, I. Igarashi, K. Fujisaki, Hiroshi Suzuki, X. Xuan","doi":"10.32268/JPROTOZOOLRES.20.1_59","DOIUrl":"https://doi.org/10.32268/JPROTOZOOLRES.20.1_59","url":null,"abstract":"","PeriodicalId":22861,"journal":{"name":"The Journal of protozoology research","volume":"20 1","pages":"59-69"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86209980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：