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Analysis of Physiochemical Characteristics of Angelica acutiloba Roots by Different Drying Methods 不同干燥方法对当归根理化特性的分析
Pub Date : 2023-10-31 DOI: 10.7783/kjmcs.2023.31.5.297
Dae Hui Jeong, Hong-Woo Park, Young-Ki Kim, Hae-Yun Kwon
Background: This study was conducted to confirm the morphological and compositional changes in Angelica acutiloba (Siebold & Zucc.) Kitagawa roots by different drying methods and to provide basic data for post-harvest management.BRMethods and Results: Two types of root samples, fine roots that were originally removed and samples that were sliced, were prepared. They were dried by four methods (freeze-drying, hot airdrying, shade-drying, and direct solar-drying), and the drying characteristics were investigated. Drying time was the shortest in freeze-drying, and the longest in shade-drying. The hardness of the dried roots was relatively higher in hot air- and direct solar-dried samples, and the color varied according to each drying treatments. The (Z)-ligustilide content was relatively higher in the case of freeze-dried or shade-dried samples than in other samples.BRConclusions: These results provide insights into the optimal post-harvest drying technology for A. acutiloba.
背景:本研究旨在证实当归(Angelica acutiloba, Siebold & Zucc.)的形态和成分变化。通过对北川根不同干燥方法的研究,为采收后的管理提供基础数据。方法与结果:制备了两种类型的根样品,一种是原始切除的细根,另一种是切片的根。采用冷冻干燥、热风干燥、遮荫干燥和日光直接干燥四种干燥方法对其进行干燥,并对其干燥特性进行了研究。干燥时间以冷冻干燥最短,阴凉干燥最长。热风干燥和日光直接干燥的根的硬度相对较高,不同干燥方式的根的颜色也不同。冻干和阴凉干燥样品的(Z)-藁本内酯含量相对高于其他样品。结论:研究结果为金针叶采收后最佳干燥工艺提供了依据。
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引用次数: 0
Neuroprotective Effects of Korean Ginseng Berry against Hydrogen Peroxideinduced Oxidative Damage in SH-SY5Y Neuroblastoma Cells 高丽人参对SH-SY5Y神经母细胞瘤细胞过氧化氢氧化损伤的保护作用
Pub Date : 2023-10-31 DOI: 10.7783/kjmcs.2023.31.5.333
Seul-A Jin, Su-Kang Kim, Ju Yeon Ban
Background: Studies on the efficacy of ginseng have focused on the ginseng root. Recently, research on ginseng berries has expanded, revealing its efficacy in liver disease, obesity, and diabetes. This study aimed to examine the neuroprotective effects of Korean ginseng berry (KGB) extract on hydrogen peroxide (H₂O₂)-induced oxidative stress in human neuroblastoma SH-SY5Y cells.BRMethods and Results: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, reactive oxygen species (ROS) generation, mitochondrial membrane potential, and protein expression analysis were used to evaluate the cytotoxicity and inhibition of apoptosis in SH-SY5Y cells. KGB pretreatment reduced ROS production and mitochondrial dysfunction induced by H₂O₂-mediated apoptosis in human neuroblastoma SH-SY5Y cells, while also inhibiting Bax upregulation, Bcl-2 downregulation, and caspase-3 activation. Moreover, KGB treatment increased the activity of antioxidant enzymes including superoxide dismutase, glutathione peroxidase, and catalase and decreased the malondialdehyde content.BRConclusions: This study found that KGB protected human neuroblastoma SH-SY5Y cells from H₂O₂-induced oxidative stress by inhibiting the MAPK signaling pathway. Based on these findings, KGB may be a potential therapeutic agent for preventing and treating neurodegenerative diseases and other disorders associated with oxidative stress.
背景:对人参功效的研究主要集中在人参根上。最近,对人参浆果的研究扩大,揭示了它对肝病、肥胖、糖尿病的功效。本研究旨在探讨高丽人参提取物(KGB)对过氧化氢(H₂O₂)诱导的人神经母细胞瘤SH-SY5Y细胞氧化应激的保护作用。方法和结果:采用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑试验、活性氧(ROS)生成、线粒体膜电位和蛋白表达分析来评价SH-SY5Y细胞的细胞毒性和凋亡抑制作用。KGB预处理可减少人神经母细胞瘤SH-SY5Y细胞h2o2介导的凋亡诱导的ROS生成和线粒体功能障碍,同时抑制Bax上调、Bcl-2下调和caspase-3活化。此外,KGB处理提高了抗氧化酶包括超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶的活性,降低了丙二醛含量。结论:本研究发现KGB通过抑制MAPK信号通路保护人神经母细胞瘤SH-SY5Y细胞免受h2o2诱导的氧化应激。基于这些发现,KGB可能是预防和治疗与氧化应激相关的神经退行性疾病和其他疾病的潜在治疗剂。
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引用次数: 0
Development of Mitocondrial Genome-based InDel Markers to Distinguish between Ganoderma lucidum and Perenniporia fraxinea 基于线粒体基因组的InDel标记鉴别灵芝和多年生茯苓
Pub Date : 2023-10-31 DOI: 10.7783/kjmcs.2023.31.5.290
Hwa Yong An, Hyun-Jo Koo, Eun-Kyoung Kim, Ji-Ran Jeong, Gyoung-Ju Nah
Background: Ganoderma lucidum and Perenniporia fraxinea are morphologically similar, making them difficult to distinguish when cut into pieces or powdered. Ganoderma lucidum is a more effective medicinal mushroom than P. fraxinea, but it is expensive, so there is a strong possibility that similar shaped mushrooms are mixed and sold together. Therefore, molecular biological markers that can differentiate G. lucidum from P. fraxinea are needed.BRMethods and Results: We selected two polymorphic insertion-deletion (InDel) sites and one polymorphic InDel site from the cox1 and rnl genes within the mitochondrial DNA, respectively, and designed primers using flanking sequences that surrounded these polymorphic sites. We performed a polymerase chain reaction (PCR) to validate these markers. Three InDel markers were developed to differentiate between G. lucidum and P. fraxinea: GlPf_INDEL01, GlPf_INDEL02, and GlPf_INDEL04.BRConclusions: The three InDel markers developed in this study combined with a simple PCR analysis easily distinguished G. lucidum from P. fraxinea.
背景:灵芝和多年生茯苓在形态上很相似,因此当它们被切成片或粉末状时很难区分。灵芝是一种比黄假单胞菌更有效的药用蘑菇,但价格昂贵,因此很有可能将形状相似的蘑菇混合在一起出售。因此,需要有分子生物学标记来区分灵芝和凤梨。方法与结果:我们分别从线粒体DNA的cox1和rnl基因中选择了两个多态插入缺失(InDel)位点和一个多态InDel位点,并利用这些多态位点周围的侧翼序列设计引物。我们进行了聚合酶链反应(PCR)来验证这些标记。建立了3个InDel标记,分别为GlPf_INDEL01、GlPf_INDEL02和GlPf_INDEL04。结论:本研究建立的3个InDel标记结合简单的PCR分析,可以很容易地将G. lucidum与P. fraxinea区分开来。
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引用次数: 0
A Study on the Pharmacological Problems and Glycyrrhizin of Licorice Distributed in Korea 韩国产甘草药理问题及甘草酸的研究
Pub Date : 2023-10-31 DOI: 10.7783/kjmcs.2023.31.5.324
Yong-Il Kim, Dong-Kyun Son, Jin Tae Jung, Eun-Song Lee, Yong-Goo Kim, Kyung Sook Han, Kyung Ho Ma, Young Ho Yoon, Jeong Hoon Lee
Background: Scarcity of wild licorice, which is most commonly used in oriental medicine, has led to an increase in the use of various other licorice species. Diversification of licorice species can affect the quality, safety, and standardization of food and medicine; therefore, it is crucial to verify and evaluate their major pharmacological components.BRMethods and Results: In this study, we collected licorice produced and distributed in various regions of Asia, including Korea, and compared the content of glycyrrhizin. The average glycyrrhizin content of wild licorice produced in China (Yangoe), Uzbekistan, Kyrgyzstan, and Kazakhstan was 3.2%, 5.5%, 5.5%, and 5.3%, respectively. In contrast, the average glycyrrhizin content of cultivated licorice from Xinjiang, China and Jecheon, Korea was 4.8% and 0.8%, respectively. The glycyrrhizin content of each licorice slice ranged from 1.0% to 12.4%. Wild licorice, in particular, had a high glycyrrhizin content and variation. In addition to pharmacological effects, glycyrrhizin has various side effects; therefore, the quality of wild licorice, which has been traditionally regarded as good, needs to be re-evaluated.BRConclusions: In terms of the stability of food and pharmaceutical raw materials, licorice with uniformity and appropriate content of glycyrrhizin is more effective in controlling and utilizing the pharmacology than licorice with considerably high glycyrrhizin content. To this end, it is crucial to shift production from wild licorice collection to cultivated licorice and develop related cultivation technologies.
背景:在东方医学中最常用的野生甘草的短缺导致了各种其他甘草品种的使用增加。甘草品种的多样化会影响食品和药品的质量、安全和标准化;因此,验证和评估其主要药理成分是至关重要的。方法与结果:在本研究中,我们收集了包括韩国在内的亚洲不同地区生产和分布的甘草,并比较了甘草酸的含量。中国(yanggoe)、乌兹别克斯坦(Uzbekistan)、吉尔吉斯斯坦(Kyrgyzstan)和哈萨克斯坦(Kazakhstan)产野生甘草中甘草酸的平均含量分别为3.2%、5.5%、5.5%和5.3%。相比之下,中国新疆和韩国堤川地区栽培甘草的甘草酸平均含量分别为4.8%和0.8%。甘草片中甘草酸含量为1.0% ~ 12.4%。尤其是野生甘草,其甘草酸含量和变异率都很高。除了药理作用外,甘草酸还有各种副作用;因此,传统上被认为是好的野生甘草的质量需要重新评估。结论:在食品和医药原料的稳定性方面,甘草酸含量均匀且适宜的甘草比甘草酸含量相当高的甘草更能有效地控制和利用药理学。为此,从野生甘草生产向栽培甘草生产转变,并发展相关栽培技术至关重要。
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引用次数: 0
Comparative Analysis of Morphological Characteristics and Effective Components of Domestic Codonopsis Plants 国产党参植物形态特征及有效成分的比较分析
Pub Date : 2023-10-31 DOI: 10.7783/kjmcs.2023.31.5.283
Sung Cheol Koo, Jin Yu, Bok Jang
Background: Among the Codonopsis plants, C. lanceolata and C. pilosula are cultivated for edible and medicinal purposes. But basic agronomic data of C. ussuriensis are required for domestic cultivation. This study aimed to compare the agronomic characteristics of the three Codonopsis species for cultivation.BRMethods and Results: The three Codonopsis species were cultivated in the same field and their agronomic characteristics were compared. C. ussuriensis demonstrated similar morphological characteristics to C. pilosula, such as hairless stems and leaves, stem color, wingless seeds, and root weight. However, the shape and size of the leaf and the color of the corolla are intermediate between C. lanceolata and C. pilosula. However, all three species showed different root shapes and seed sizes and weights. In terms of phytochemical components, the main components of C. lanceolata and C. pilosula were tangshenoside I and lobetyolin, while C. ussuriensis was ussurienoside I. Moreover, all components demonstrated high content with increasing annual age.BRConclusions: This study demonstrated different agronomic characteristics of C. ussuriensis compared to C. lanceolata and C. pilosula, and the results are valuable as basic data for cultivating C. ussuriensis.
背景:在党参属植物中,杉木党参(C. lanceolata)和党参党参(C. pilosula)既可食用又可药用。但要进行国内栽培,还需要具备基本的农艺资料。本研究旨在比较三种党参栽培的农艺性状。方法与结果:对三种党参在同一农田进行栽培,比较其农艺性状。乌苏里草的茎叶无毛、茎色、种子无翅、根重等形态特征与党参相似。然而,叶片的形状和大小以及花冠的颜色介于杉木和党参之间。然而,这三个物种的根形状、种子大小和重量都不同。在植物化学成分方面,杉木和党参的主要成分为汤参苷I和枇杷苷,乌苏里木的主要成分为乌苏里苷I,且随着年龄的增加,各成分含量均较高。结论:本研究显示了乌苏里松与杉木、党参不同的农艺性状,为乌苏里松的栽培提供了有价值的基础数据。
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引用次数: 0
Development of Genomic Simple Sequence Repeat Markers and Variety Discrimination in Salvia miltiorrhiza Bunge 丹参基因组简单序列重复标记的开发及品种鉴别
Pub Date : 2023-10-31 DOI: 10.7783/kjmcs.2023.31.5.304
Ei Hyun Kim, Ma-Kyung Ho, Jeong Hoon Lee, Jeong-Jin Tae, Han-Jong Won, Jong Wook Chung
Background: Salvia miltiorrhiza Bunge, of the Lamiaceae family, is a medicinal plant. This study aimed to develop polymorphic simple sequence repeat (SSR) markers for analyzing the genetic diversity and distinguishing between varieties of S. miltiorrhiza.BRMethods and Results: Using whole genome resequencing data, 50 SSR markers were designed for S. miltiorrhiza. Of these, 41 polymorphic markers were selected and utilized to assess 44 S. miltiorrhiza accessions. A total of 346 alleles were detected (2 to 17 per locus, averaging 8.4). Major allele frequency ranged from 0.19 to 0.83 (average 0.47), observed heterozygosity ranged from 0 to 0.60 (average 0.22), and polymorphic information content ranged from 0.29 to 0.87 (average 0.64). Among the 41 SSR markers, 18 were effective for distinguishing varieties, particularly S. miltiorrhiza ‘Dasan’, ‘Gosan’, and
背景:丹参属丹参科,是一种药用植物。本研究旨在建立多态简单序列重复(SSR)标记,用于分析丹参品种间的遗传多样性和区分。方法与结果:利用全基因组重测序数据,设计了50个SSR标记。从中选择41个多态性标记,对44份丹参材料进行了评价。共检测到346个等位基因(每个位点2 ~ 17个,平均8.4个)。主等位基因频率范围为0.19 ~ 0.83(平均0.47),观察到的杂合度范围为0 ~ 0.60(平均0.22),多态信息含量范围为0.29 ~ 0.87(平均0.64)。在41个SSR标记中,有18个标记对品种有较好的鉴别效果,其中以丹参‘茶山’、‘高山’和‘高山’最为显著
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引用次数: 0
Analysis of Wing Forms and Dominant of Thrips nigropilosus Uzel (Thysanoptera: Thripidae) Inflicting to Cnidium officinale Makino 黑尾蓟马翅型及优势对马野刺金蝇的影响分析
Pub Date : 2023-10-31 DOI: 10.7783/kjmcs.2023.31.5.316
Jung Beom Yoon, Deok-Ho Kwon, Chung Ryul Jung
Background: The study aimed to investigate its wing forms and dominant species of thrips inflicting C. officinale.BRMethods and results: T. nigropilosus was collected by the biting method in a total 17 different cultivation sites during 2020 - 2022 and the species was identified by stereo microscope. A Total eight species including T. nigropilosus, T. tabaci, Haplothrips chinensis, Frankliniella intonsa, T. palmi, Microcephalothrips abdominalis, F. occidentalis and Anaphothrips obscurus were identified in four and thirteen cultivation sites in 2020 and 2022, respectively. T. nigropilosus was the more abundant species than T. tabaci. Their wing form was divided into macropterous (long-winged) and brachypterous (short-winged) and the macropterous individuals were dominant than brachypterous.BRConclusions: The dominant species is chrysanthemum thrips, Thrips nigropilosus and it has two wing forms discriminated as macropterous and brachypterous.
背景:本研究的目的是为了了解其翅的形式和优势种的thrips施加C. officinale。方法与结果:2020 - 2022年,在17个不同养殖地点采用咬捕法采集黑绒拟蚊,并用立体显微镜对其进行鉴定。2020年和2022年分别在4个和13个养殖点位鉴定出黑毛恙虫、烟粉虱、中国单倍蓟马、鼠唇虱、棕榈蓟马、腹部小头蓟马、西方蓟马和暗拟蓟马8种。黑毛蜱的种类比烟粉虱丰富。翅型分为大翅类(长翅)和短翅类(短翅),大翅类个体比短翅类个体占优势。结论:优势种为菊花蓟马(thrps nigropilosus),有大翅类和短翅类两种翅型。
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引用次数: 0
Radical Scavenging Active Compound Analysis in Extract of Coffee Silver Skin 咖啡银皮提取物中自由基清除活性化合物分析
Pub Date : 2023-08-31 DOI: 10.7783/kjmcs.2023.31.4.211
Kyoung-In Lee, Ji-Hwan Back, Byoung-Sik Pyo, Chul-Hee Choi
{"title":"Radical Scavenging Active Compound Analysis in Extract of Coffee Silver Skin","authors":"Kyoung-In Lee, Ji-Hwan Back, Byoung-Sik Pyo, Chul-Hee Choi","doi":"10.7783/kjmcs.2023.31.4.211","DOIUrl":"https://doi.org/10.7783/kjmcs.2023.31.4.211","url":null,"abstract":"","PeriodicalId":22918,"journal":{"name":"The Korean Journal of Medicinal Crop Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135988576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of Plug Tray Size, Light Intensity, and Fertilization Condition on Seedling Growth of Pseudolysimachion longifolium (L.) Opiz 塞盘大小、光照强度和施肥条件对长叶假水仙幼苗生长的影响Opiz
Pub Date : 2023-08-31 DOI: 10.7783/kjmcs.2023.31.4.254
Hyuck-Hwan Kwon, Suvi Kim, Jun-Kyu Bae, Wonwoo Cho
{"title":"Effects of Plug Tray Size, Light Intensity, and Fertilization Condition on Seedling Growth of Pseudolysimachion longifolium (L.) Opiz","authors":"Hyuck-Hwan Kwon, Suvi Kim, Jun-Kyu Bae, Wonwoo Cho","doi":"10.7783/kjmcs.2023.31.4.254","DOIUrl":"https://doi.org/10.7783/kjmcs.2023.31.4.254","url":null,"abstract":"","PeriodicalId":22918,"journal":{"name":"The Korean Journal of Medicinal Crop Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135988581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Applying Multivariate Statistical Analysis for Agronomic Characteristics to Evaluate Leaf Availability in Perilla Germplasms 应用农艺性状多元统计分析评价紫苏种质叶片有效性
Pub Date : 2023-08-31 DOI: 10.7783/kjmcs.2023.31.4.222
Myoung-Hee Lee, Jung-Sook Sung, Kwang-Soo Cho, Chun-Song Kim, Eun-Soo Lee, Jung-In Kim, Eun-Ae Yoo, Sang-Woo Kim, Sung-Up Kim, Eun-Young Oh, Min-Young Kim, Jung-Eun Lee
{"title":"Applying Multivariate Statistical Analysis for Agronomic Characteristics to Evaluate Leaf Availability in Perilla Germplasms","authors":"Myoung-Hee Lee, Jung-Sook Sung, Kwang-Soo Cho, Chun-Song Kim, Eun-Soo Lee, Jung-In Kim, Eun-Ae Yoo, Sang-Woo Kim, Sung-Up Kim, Eun-Young Oh, Min-Young Kim, Jung-Eun Lee","doi":"10.7783/kjmcs.2023.31.4.222","DOIUrl":"https://doi.org/10.7783/kjmcs.2023.31.4.222","url":null,"abstract":"","PeriodicalId":22918,"journal":{"name":"The Korean Journal of Medicinal Crop Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135988578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
The Korean Journal of Medicinal Crop Science
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