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Oral diseases are often caused by bacterial infections, making the inhibition of receptors like N-acetylglucosamine critical in preventing bacterial formation. The plant Myrmecodia pendans (M. pendans) is known for its diverse bioactivities and may serve as a promising source for developing new antibacterial agents. This study employs in silico methods to predict the inhibitory mechanisms, pharmacokinetics, and drug-likeness of compounds isolated from M. pendans. Three compounds were evaluated for their inhibitory effects on the MurA and MurB receptors using the AutoDock4 molecular docking software, with visualizations performed using the BIOVIA Discovery Studio Visualizer. The binding affinities obtained for compounds 1, 2, and 3 to the MurA receptor were -9.42, -9.57, and -6.84 kcal/mol, respectively, while their binding affinities to the MurB receptor were -11.25, -10.55, and -8.69 kcal/mol. These affinities were found to be stronger than those of fosfomycin (benchmark compound) but weaker than the native ligands of the respective receptors. Key amino acid residues involved in the binding to MurA were identified as Cys115 and Asp305, while Ser82 and Asn83 were noted for MurB. In the ADMET prediction and drug-likeness analysis, some compounds met the necessary criteria, whereas others did not. Although all the three compounds demonstrated strong predicted inhibitory activity against MurA and MurB receptors, the analysis suggests that Compound 2 may hold the most promise as a potential antibacterial agent, warranting further investigation.

The main aim of this work was to assess the potential formation of ternary chelate complexes, involving deferasirox (DFX), ciprofloxacin (CP), and ferric iron. The coadministration of CP along with DFX might modulate its efficacy, so it is important that it be investigated. A ternary complex involving DFX, CP, and iron (DFX-CP-Fe) was prepared and characterized. Theoretical chemistry calculations were performed to measure the equilibrium constants of complexes. Two groups of rats were exposed to DFX or DFX with CP. The level of DFX in plasma was measured, and histological assessments of relevant organs were made. Levels of iron in selected tissues were measured. The formation of ternary complexes was confirmed. Two ternary complexes are thermodynamically favored. The first one of ratio (DFX:CP:Fe) was shown to be favorable with an equilibrium constant of 2 × 10⁷. The second one with ratio (DFX:CP:2Fe) is more thermodynamically favored with an equilibrium constant of 2.0 × 10⁶⁰. Rats treated with a combination of DFX and CP exhibited lower levels of iron in dried red blood cells in comparison to those treated with DFX alone (p value = 0.012). They also exhibited lower levels of DFX in plasma. Histological assessments of the relevant tissues showed a clear difference in the level of deposited iron in the spleen. In conclusion, ternary complexes are formed, and some with exceptionally high constants. The obtained data indicate a potentially favorable role of CP because while it resulted in a decrease in the level of DFX, it pharmacodynamically produced more effect.

The future of groundwater is one of the key challenges for sustainable water management, hence the need to monitor its overall quality. The objective of this work is to assess the overall quality and determine the spatiotemporal evolution of the Angads aquifer in northeastern Morocco in 2014 and 2020, based on the parameters NH₄ ⁺, NO₃ ^-, EC, Cl^-, and FC, as well as the Geographic Information System (GIS). The results of the comparison of these five parameters between 2014 and 2020 show a general increase in NH₄ ⁺ and a decrease in NO₃ ^- and FC at most sampling points. These changes could be attributed to a shift in pollution sources or biological processes affecting water quality. On the other hand, the stability of EC and Cl^- levels suggests a consistency in the inputs of salts or minerals. The quality percentages show a decrease in good, poor, and very poor quality, following an increase in average quality, from 10.52% (in 2014) to 5.26% (in 2020), 31.57% (in 2014) to 21.05% (in 2020), 31.57% (in 2014) to 26.31% (in 2020), and 26.31% (in 2014) to 47.36% (in 2020), respectively. Spatial and temporal mapping of the quality over these 2 years shows that the deterioration continues toward the east, southeast, and southwest. This is justified by very high measurements of the parameters NO₃ ^-, EC, and Cl^- at sampling points 2, 3, 4, 5, 7, 8, and 15 for 2014 and 2020, reaching 156 mg/L, 10,570 µS/cm, and 3790 mg/L in 2014 and 134 mg/L, 10,355 µS/cm, and 3597 mg/L in 2020, respectively, due to effluents from pollution points such as the Oujda public landfill, the wastewater treatment plant, and the former Sidi Yahya landfill to the west. On the other hand, in the north, northeast, and northwest, there has been an improvement in quality due to the remoteness of these pollution points. In order to protect this vital resource, recommendations need to be put in place, in particular by treating leachates so as to ensure that the quality of the water is not discharged directly into the aquifer or used for other purposes, and to avoid discharging effluent from the wastewater treatment plant into the natural environment.

Background and Objectives: Contemporary dentistry focuses on more conservative treatment options such as endocrown restorations and application of dental materials with higher resemblance to tooth structure. Polyether ether ketone (PEEK) polymer is a material used for the fabrication of endocrowns. This study aimed to compare the post-fatigue resistance (PFR) of mandibular first molars restored with PEEK and lithium disilicate (LS₂) endocrown restorations. Materials and Methods: This in vitro, experimental study was conducted on 20 human mandibular first molars with similar dimensions. The teeth were prepared for endocrown restoration and were assigned to two groups (n = 10) of PEEK and LS₂ endocrowns. After fabrication by the computer-aided design and computer-aided manufacturing (CAD-CAM) technique, the restorations were cemented with resin cement. Next, the teeth underwent 15,000 thermal cycles followed by cyclic loading with 600,000 cycles of compressive force (100 N, 4 Hz) and were then subjected to compressive load application in a universal testing machine. The load causing endocrown failure was recorded as the PFR of the respective restoration. The failure mode was also inspected under a light microscope. Data were analyzed by the independent t-test and also chi-square, Mann-Whitney, and Fisher's exact tests (α < 0.05). Results: The teeth with PEEK endocrowns showed significantly higher PFR than those with LS₂ endocrowns. Irreparable fractures were dominant in both groups. Conclusion: PEEK may serve as a suitable alternative to LS₂ for the fabrication of endocrown restorations.

Background: Food chemical antioxidants have demonstrated protective effects against reactive oxygen species and free radicals, but present in excess, harmful consequences might occur on health. Therefore, replacing these synthetic additives with nontoxic natural antioxidants is crucial. Objective: The current study examined aroma profile, antioxidant activity, and multivariate analysis of Mentha piperita, Mentha pulegium, Thymus serpyllum, and Thymus zygis essential oils from Morocco. Methods: GC-MS analysis was carried out to determine the chemical composition of the four oils, and their antioxidant activity was evaluated with 2,2-diphenyl-1-picrylhydrazyl (DPPH), cation radical (ABTS⁺), hydrogen peroxide scavenging capacity (H₂O₂), ferric reducing antioxidant power (FRAP), and total antioxidant capacity (TAC) methods. Results: Isomintlactone (35.55%), pulegone (74.04%), borneol (37.87%), and borneol (30.99%) were the most abundant compounds of M. piperita, M. pulegium, T. serpyllum, and T. zygis EOs. The antioxidant activity of the four EOs was particularly notable, with an IC₅₀ varying between 3.51 ± 0.22 mg/mL and 0.49 ± 0.08 mg/mL by the DPPH method, 1.02 ± 0.21 mg/mL and 0.4 ± 0.7 mg/mL by the ABTS method, and 0.063 ± 0.01 mg/mL and 0.009 ± 0.008 mg/mL by the H₂O₂ method. For the FRAP technique, the EC₅₀ was between 0.42 ± 0.02 mg/mL and 0.09 ± 0.01 mg/mL. Finally, the equivalent concentration of ascorbic acid ranged between 10.42 ± 0.03 mg AAs/mL for M. piperita and 7.25 ± 0.19 mg AAs/mL for T. serpyllum. As determined by multivariate analysis, antioxidant activities through the DPPH, ABTS, TAC, and FRAP were mainly influenced the major compounds of M. pulegium and M. piperita EOs. However, the H₂O₂ method showed a stronger positive correlation with major compounds of T. zygis EO. Conclusion: The EOs derived from M. piperita, M. pulegium, T. serpyllum, and T. zygis species might be exploited as a natural source for antioxidant activity.

Hair is an intricate biological structure that originates from hair follicles (HFs), which are complex mini-organs embedded in the skin. Each HF undergoes continuous cycles of growth (anagen), regression (catagen), and rest (telogen), driven by intricate signaling pathways and interactions between epithelial and mesodermal cells. The development of HFs requires the interplay of several key signaling pathways, including Wnt, Shh, Notch, and BMP. The Wnt pathway is primarily involved in induction, Shh is essential for early organogenesis and later stages of cytodifferentiation, Notch signaling governs the fate of HF stem cells, and BMP plays a role in cytodifferentiation. Hair health is closely associated with psychological well-being and personal distress. While hair loss (alopecia) does not impact biological health, it significantly affects social well-being. Therefore, a deep understanding of the molecular mechanisms underlying HF development is crucial for developing treatments for hair-related problems and improving hair health. This knowledge has led to significant advancements in therapeutic applications, particularly in treating hair loss disorders, enhancing wound healing, and developing cosmetic treatments. This paper aims to review the molecular mechanisms involved in HF development, with an emphasis on their potential impact on human health and well-being.

Plants require essential macronutrients such as nitrogen (N), phosphorus (P), and potassium (K), but their availability in soil is often inefficient due to evaporation, leaching, and binding. Controlled-release fertilizers (CRFs) provide a solution by regulating nutrient release over time. This study evaluates the effects of two coating materials, polyethylene glycol (PEG) and humic acid, on the release patterns of N, P, and K during an 18-week incubation using Inceptisol from Bogor, Indonesia. Various CRF treatments were tested, including uncoated (A1, A2), PEG-coated (B1, B2), and humic acid-coated (C1, C2) formulations. Results showed that CRF with PEG (B2) demonstrated slower N release, with ammonium levels decreasing from 32.22% in week 1 to 9.36% by week 18. Nitrate release increased steadily from 26.37% to 37.36% between weeks 3 and 18. In contrast, CRF with humic acid (C2) showed slower nitrate release, reaching 36.26% by the end of incubation. P release patterns were similar across treatments, while K release was lowest in the humic acid-coated treatment (C2) at 24.48%. These findings underline the potential of coating materials like PEG and humic acid to optimize nutrient release, enhancing agricultural efficiency.

Applying inorganic phosphorus fertilizer is less effective in increasing crop yields in tropical soils due to precipitation and adsorption reactions. However, research suggests that partial substitution of organic and inorganic fertilizers has shown to improve the efficiency of applied phosphorus fertilizer by reducing its adsorption and enhancing desorption due to their synergistic effects. This study aimed to investigate the impact of treating the soil with mixed manure (MM) rates and blended nitrogen, phosphorus, sulfur, and boron (NPSB) fertilizer on the soil's phosphorus adsorption and desorption characteristics. Results showed increased adsorbed phosphorus in all treatments, with increased added phosphorus (P) concentration from 100 to 500 mg kg^-1. However, the efficiency of adsorbed P decreased significantly as added P concentration rates increased from 100 to 400 mg·kg^-1 in all treatments and then decreased as the added P concentration advanced to 500 mg·kg^-1. Moreover, in all treatments that received combined applications of MM and blended NPSB, both quantity and percentage of desorbed P showed a significant increase. The Freundlich adsorption coefficient and constant were also significantly reduced because of the combined application of MM and blended NPSB, compared to the control and their sole applications. Overall, the soil treated with a combined application of 15 t·ha^-1 of MM with 100 kg·ha^-1 of blended NPSB showed the highest reduction in the efficiency of adsorbed P, percentage of desorbed P, Freundlich adsorption capacity, and intensity by 8%, 37.5%, 60%, and 58%, respectively, as compared to the control. These findings indicate that the combined application of MM and blended NPSB can improve the P availability and uptake by maize by reducing its adsorption while increasing desorption characteristics. Finally, this experiment recommends further research on the long-term effects of MM and blended NPSB on P adsorption and desorption characteristics of vertisols.

Moringa oleifera (MO) has been an important plant for food and traditional medicine in Asian countries, including Indonesia. The leaves of these plants are reported to be rich in antioxidants, vitamins, and micronutrients and have been proven to have nootropic properties. Therefore, we investigated whether MO could provide protective effects on SH-SY5Y neuroblastoma cells exposed to H₂O₂. In this study, we observed cotreating water-extracted MO leaves on the inhibition of reactive oxygen species (ROS). We found that this treatment enhanced the activities of glutathione peroxidase, catalase, and superoxide dismutase. In addition, it suppressed the mRNA expression levels of apoptotic gene-related genes, specifically Bcl-2 associated protein X (BAX) and caspase 3. Furthermore, it promoted neuroplasticity by increasing the brain-derived neurotropic factor (BDNF) mRNA expression in SH-SY5Y cells. The protein expression of phosphorylated-Akt and phosphorylated-CREB, essential genes in neuroplasticity, was also increased in cells treated with H₂O₂ and MO. Therefore, the neuroprotective effects of MO against oxidative stress are attributed to its antioxidant and antiapoptotic properties, as well as its ability to modify the neuronal signaling pathway.

Background: Currently, there is no gold standard technique in SCI therapy. Although there have been many systematic reviews on the pharmacological treatment of inflammation in SCI, there has been no published discussion regarding the effectiveness of anti-inflammatory pharmacotherapy when viewed from a neuroinflammatory pathway. This research aimed to examine an effective and reliable medication for decreasing inflammation in SCI and, where possible, identify effective pharmacotherapeutic treatment protocols. Methods: We searched SCOPUS, PubMed, PlosOne, and Science Direct, for experimental trials published in English. The keywords included (Pharmacotherapy OR pharmacology OR treatment) AND (traumatic spinal cord injury OR spinal cord OR traumatic OR hemisection of spinal cord OR compression OR traumatic spinal cord injury OR aneurysm clip OR hemisection of spinal cord OR sharp pin injury) AND (Side Effect therapy). In addition, the Basso-Beattie-Bresnahan (BBB) score was used to assess post-SCI clinical progress. Results: Twelve pilot studies met the inclusion criteria. The highest BBB score was 15, and the best animal performance was tested after the average therapy was on the second week or 14th day. Three pharmacotherapies have fast effectiveness regarding BBB scores: calcitriol, lithium, and valproic acid. As well as a combination of pharmacotherapy therapy with surgical therapy also get significant results. Conclusions: The preliminary findings showed that many inflammatory pathways cause inflammatory agents to have their pathways for inhibition pathways, so they have different ways.