Triple negative breast cancer has a high frequency of BRCA1 gene mutations. In this experiment, we examined whether there are cells that are not led to apoptosis in different subtypes of breast cancer with poor prognosis with BRCA1 mutation and wild type BRCA cells. Cells with BRCA1 wild-type （ MDA-MB-231 and BT-549 ） or mutated （ MDA-MB-436 ） BRCA1 were exposed to anticancer drugs, and the levels of reactive oxygen species （ ROS ） produced by oxidative stress and Annexin V （ an index of apoptosis ） were examined. The wild-type MDA-MB-231 cells showed increased ROS levels and Annexin V after exposure to eribulin and paclitaxel. Hence, the pathway leading to apoptosis may be activated by oxidative stress. ROS levels in BT-549 cells were significantly increased after exposure to eribulin and paclitaxel. However, there was no change in Annexin V. BRCA1-mutated MDA-MB-436 cells showed significantly increased ROS levels after exposure to eribulin and paclitaxel and no change in the Annexin V levels. This suggests that BRCA1 wild-type BT-549 cells and BRCA1-muted MDA-MB-436 cells were resistant to ROS-mediated apoptosis. These results indicate that BRCA1 mutation and cell subtypes should be investigated prior to selecting the chemotherapy combination to enable appropriate selection in clinical practice.
{"title":"BRCA1 mutated cells are less likely to undergo ROS-mediated apoptosis after exposure to eribulin and paclitaxel","authors":"Akiko Sasaki, Y. Tsunoda, Yuriko Inoue","doi":"10.15369/sujms.33.118","DOIUrl":"https://doi.org/10.15369/sujms.33.118","url":null,"abstract":"Triple negative breast cancer has a high frequency of BRCA1 gene mutations. In this experiment, we examined whether there are cells that are not led to apoptosis in different subtypes of breast cancer with poor prognosis with BRCA1 mutation and wild type BRCA cells. Cells with BRCA1 wild-type （ MDA-MB-231 and BT-549 ） or mutated （ MDA-MB-436 ） BRCA1 were exposed to anticancer drugs, and the levels of reactive oxygen species （ ROS ） produced by oxidative stress and Annexin V （ an index of apoptosis ） were examined. The wild-type MDA-MB-231 cells showed increased ROS levels and Annexin V after exposure to eribulin and paclitaxel. Hence, the pathway leading to apoptosis may be activated by oxidative stress. ROS levels in BT-549 cells were significantly increased after exposure to eribulin and paclitaxel. However, there was no change in Annexin V. BRCA1-mutated MDA-MB-436 cells showed significantly increased ROS levels after exposure to eribulin and paclitaxel and no change in the Annexin V levels. This suggests that BRCA1 wild-type BT-549 cells and BRCA1-muted MDA-MB-436 cells were resistant to ROS-mediated apoptosis. These results indicate that BRCA1 mutation and cell subtypes should be investigated prior to selecting the chemotherapy combination to enable appropriate selection in clinical practice.","PeriodicalId":23019,"journal":{"name":"The Showa University Journal of Medical Sciences","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84664150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, a highly sensitive method to simultaneously quantify the integrase strand transfer inhibitors (INSTIs) raltegravir, dolutegravir, elvitegravir, and bictegravir, which are recommended drugs in the HIV treatment guidelines, was established using liquid chromatography–tandem mass spectrometry (LC-MS/MS). Raltegravir-d3 was used as the internal standard substance. The plasma samples were deproteinized with methanol and analyzed by LC-MS/MS. Chromatographic separation was performed using the gradient method with a mobile phase A (20 mmol/l ammonium formate water) and mobile phase B (20 mmol/l ammonium formate methanol). In addition, an InertSustain C18 column (3 μm, 100 × 2.1 mm), a ow rate of 0.45 ml/min, and a measurement time of 10 minutes were used. The calibration curve showed linearity (r> 0.9904) within the range of 0.51,250 ng/ml, and the limit of quanti cation was 0.5 ng/ml for all drugs. The mean intraand inter-day accuracy was 99.6%± 7.2% and 101.0%± 5.0%, respectively, and the coef cient of variation (CV) was ≤ 18.5% and ≤ 10.3%, respectively. This method enables the highly sensitive simultaneous analysis of INSTIs and is useful for con rming the ef cacy and safety of drugs in clinical practice.
{"title":"Simultaneous Determination of Raltegravir, Dolutegravir, Elvitegravir, and Bictegravir in Human Plasma Using High-performance Liquid Chromatography-tandem Mass Spectrometry","authors":"J. Masuda, Y. Kato","doi":"10.15369/sujms.32.91","DOIUrl":"https://doi.org/10.15369/sujms.32.91","url":null,"abstract":"In this study, a highly sensitive method to simultaneously quantify the integrase strand transfer inhibitors (INSTIs) raltegravir, dolutegravir, elvitegravir, and bictegravir, which are recommended drugs in the HIV treatment guidelines, was established using liquid chromatography–tandem mass spectrometry (LC-MS/MS). Raltegravir-d3 was used as the internal standard substance. The plasma samples were deproteinized with methanol and analyzed by LC-MS/MS. Chromatographic separation was performed using the gradient method with a mobile phase A (20 mmol/l ammonium formate water) and mobile phase B (20 mmol/l ammonium formate methanol). In addition, an InertSustain C18 column (3 μm, 100 × 2.1 mm), a ow rate of 0.45 ml/min, and a measurement time of 10 minutes were used. The calibration curve showed linearity (r> 0.9904) within the range of 0.51,250 ng/ml, and the limit of quanti cation was 0.5 ng/ml for all drugs. The mean intraand inter-day accuracy was 99.6%± 7.2% and 101.0%± 5.0%, respectively, and the coef cient of variation (CV) was ≤ 18.5% and ≤ 10.3%, respectively. This method enables the highly sensitive simultaneous analysis of INSTIs and is useful for con rming the ef cacy and safety of drugs in clinical practice.","PeriodicalId":23019,"journal":{"name":"The Showa University Journal of Medical Sciences","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80952699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Nagatomo, Takahiro Takuma, I. Tokimatsu, Y. Niki, Shun Fujiwara, Ayaka Nakata, Youhei Sasaki, S. Shimada, Megumi Watanuki, Maasa Abe, So Murai, Yuta Baba, Yukiko Kawaguchi, N. Arai, Nobuyuki Kabasawa, T. Matsui, H. Tsukamoto, Y. Uto, N. Hattori, B. Saito, K. Yanagisawa, T. Nakamaki
: Four male patients admitted to the same ward in the first half of September 201Y were identified to have respiratory syncytial virus （ RSV ） infection. Their ages ranged from 49 to 85 years （ median 72.5 ） . One patient was infected with human immunodeficiency virus and three patients had hematological malignancies. Following immuno-chromatological testing with a nasal cavity swab, RSV infection was diagnosed. Although blood and sputum cultures were performed in three patients, no significant bacteria were detected. Two cases responded to supportive therapy. However, one patient died secondary to multiple myeloma, and another patient developed pneumonia and died with an exacerbation of leukemia. infections in immunocompromised hosts are associated with a poor prognosis. diagnosis will facilitate isolation of infected individuals to prevent hospital outbreaks.
{"title":"A Cluster of Respiratory Syncytial Virus Infections in a Hospital Ward for Adult Immunocompromised Patients","authors":"Y. Nagatomo, Takahiro Takuma, I. Tokimatsu, Y. Niki, Shun Fujiwara, Ayaka Nakata, Youhei Sasaki, S. Shimada, Megumi Watanuki, Maasa Abe, So Murai, Yuta Baba, Yukiko Kawaguchi, N. Arai, Nobuyuki Kabasawa, T. Matsui, H. Tsukamoto, Y. Uto, N. Hattori, B. Saito, K. Yanagisawa, T. Nakamaki","doi":"10.15369/SUJMS.32.257","DOIUrl":"https://doi.org/10.15369/SUJMS.32.257","url":null,"abstract":": Four male patients admitted to the same ward in the first half of September 201Y were identified to have respiratory syncytial virus （ RSV ） infection. Their ages ranged from 49 to 85 years （ median 72.5 ） . One patient was infected with human immunodeficiency virus and three patients had hematological malignancies. Following immuno-chromatological testing with a nasal cavity swab, RSV infection was diagnosed. Although blood and sputum cultures were performed in three patients, no significant bacteria were detected. Two cases responded to supportive therapy. However, one patient died secondary to multiple myeloma, and another patient developed pneumonia and died with an exacerbation of leukemia. infections in immunocompromised hosts are associated with a poor prognosis. diagnosis will facilitate isolation of infected individuals to prevent hospital outbreaks.","PeriodicalId":23019,"journal":{"name":"The Showa University Journal of Medical Sciences","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84000595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Marumori, K. Hiyama, Taichi Mafune, H. Horiguchi, Kohei Ohno, Makoto Watanabe, Takeshi Aoki, M. Murakami
: Colorectal neuroendocrine carcinoma （ NEC ） is extremely rare and has a dismal prognosis. However, no standardized treatment strategy has been established for this lesion. For patients with NEC and distant metastasis, disease stabilization is difficult even after treatment with multidisciplinary strategies including chemotherapy. Here we describe a case of rectal NEC that presented as multiple liver metastases; a favorable prognosis was obtained after treatment with a multidisciplinary strategy that included surgery, irradiation, and chemotherapy. A 66-year-old male presented with diarrhea and constipation. computed tomography a rectal mass involving all of the luminal circumference, after which a diagnosis of NEC was confirmed by pathological examination. A CT scan also revealed several liver metastases in S5, S6, and S8. resection with total mesenteric excision and lymphadenectomy, including the lateral lesion was recognized. In conclusion, a multidisciplinary strategy including optimal chemotherapy seems to be important to achieve a favorable prognosis of NEC of the colorectum with distant metastasis..
{"title":"Long-term Survival of a Case of Rectal Neuroendocrine Carcinoma with Liver Metastasis","authors":"T. Marumori, K. Hiyama, Taichi Mafune, H. Horiguchi, Kohei Ohno, Makoto Watanabe, Takeshi Aoki, M. Murakami","doi":"10.15369/SUJMS.32.265","DOIUrl":"https://doi.org/10.15369/SUJMS.32.265","url":null,"abstract":": Colorectal neuroendocrine carcinoma （ NEC ） is extremely rare and has a dismal prognosis. However, no standardized treatment strategy has been established for this lesion. For patients with NEC and distant metastasis, disease stabilization is difficult even after treatment with multidisciplinary strategies including chemotherapy. Here we describe a case of rectal NEC that presented as multiple liver metastases; a favorable prognosis was obtained after treatment with a multidisciplinary strategy that included surgery, irradiation, and chemotherapy. A 66-year-old male presented with diarrhea and constipation. computed tomography a rectal mass involving all of the luminal circumference, after which a diagnosis of NEC was confirmed by pathological examination. A CT scan also revealed several liver metastases in S5, S6, and S8. resection with total mesenteric excision and lymphadenectomy, including the lateral lesion was recognized. In conclusion, a multidisciplinary strategy including optimal chemotherapy seems to be important to achieve a favorable prognosis of NEC of the colorectum with distant metastasis..","PeriodicalId":23019,"journal":{"name":"The Showa University Journal of Medical Sciences","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88749611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hidehiro Kochidaira, T. Kawakami, A. Yura, K. Takata, M. Inagaki, Y. Kiuchi, S. Iwai
: Oxidative stress is one cause of atherosclerosis that makes it a lifestyle-related disease. Oxidized low-density lipoprotein （ OxLDL ） was previously found to be related to oxidative stress, measured using the diacron-reactive oxygen metabolites （ d-ROMs ） test and showed a negative correlation between biological antioxidant potential （ BAP ） test results and triglycerides （ TG ） . In addition, large gender differences exist among vascular disorders caused by arteriosclerosis. However, such gender differences and their correlation with oxidative stress and blood lipids have not been clarified. In this study, gender differences in correlations between oxidative stress and blood lipids as factors in the development of atherosclerosis was addressed. Subjects were 149 individuals who underwent medical examinations conducted in Ashikaga Teishin Clinic in Tochigi, Japan （ 98 males and 51 females ） . A strong positive correlation was observed between d-ROMs test results and OxLDL in men （ R ＝ 0.480, P ＜ 0.0001 ） , but no correlation was seen in women. A strong negative correlation between BAP test results and TG was also noted in men （ R ＝ 0.571, P ＜ 0.0001 ） , and a moderate negative correlation was detected in women （ R ＝ 0.344, P ＝ 0.0133 ） . A positive correlation between d-ROMs tests and OxLDL was seen in women under 50 years of age （ R ＝ 0.399, P ＝ 0.0393 ） , but this correlation was not present in women who were 50 years of age or older （ R ＝ 0.00656, P ＝ 0.976 ） . Correlations between oxidative stress and OxLDL and between antioxidant potential and TG in men were more prominent than in women. This finding suggests that decreasing oxidative stress in the blood to prevent atherosclerosis is more important for men.
氧化应激是导致动脉粥样硬化的原因之一，使其成为一种与生活方式有关的疾病。氧化低密度脂蛋白(OxLDL)先前被发现与氧化应激有关，使用diacro -reactive oxygen metabolites (d-ROMs)测试测量，并显示生物抗氧化潜能(BAP)测试结果与甘油三酯(TG)之间呈负相关。此外，动脉硬化引起的血管疾病存在较大的性别差异。然而，这种性别差异及其与氧化应激和血脂的关系尚不清楚。在这项研究中，氧化应激和血脂作为动脉粥样硬化发展的因素之间的相关性的性别差异得到了解决。受试者为149人，他们在日本枥木市足贺Teishin诊所接受了医学检查(男性98人，女性51人)。在男性中，d- rom检测结果与OxLDL呈显著正相关(R = 0.480, P < 0.0001)，而在女性中无相关。BAP检测结果与TG在男性中呈显著负相关(R = - 0.571, P < 0.0001)，在女性中呈中度负相关(R = - 0.344, P = 0.0133)。在50岁以下的女性中，d-ROMs检测与OxLDL呈正相关(R = 0.399, P = 0.0393)，但在50岁及以上的女性中不存在这种相关性(R = - 0.00656, P = 0.976)。氧化应激与OxLDL、抗氧化潜能与TG之间的相关性在男性中比在女性中更为显著。这一发现表明，减少血液中的氧化应激以预防动脉粥样硬化对男性更为重要。
{"title":"Correlations between Oxidative Stress and Blood Lipids Are Stronger in Men than Women","authors":"Hidehiro Kochidaira, T. Kawakami, A. Yura, K. Takata, M. Inagaki, Y. Kiuchi, S. Iwai","doi":"10.15369/sujms.32.159","DOIUrl":"https://doi.org/10.15369/sujms.32.159","url":null,"abstract":": Oxidative stress is one cause of atherosclerosis that makes it a lifestyle-related disease. Oxidized low-density lipoprotein （ OxLDL ） was previously found to be related to oxidative stress, measured using the diacron-reactive oxygen metabolites （ d-ROMs ） test and showed a negative correlation between biological antioxidant potential （ BAP ） test results and triglycerides （ TG ） . In addition, large gender differences exist among vascular disorders caused by arteriosclerosis. However, such gender differences and their correlation with oxidative stress and blood lipids have not been clarified. In this study, gender differences in correlations between oxidative stress and blood lipids as factors in the development of atherosclerosis was addressed. Subjects were 149 individuals who underwent medical examinations conducted in Ashikaga Teishin Clinic in Tochigi, Japan （ 98 males and 51 females ） . A strong positive correlation was observed between d-ROMs test results and OxLDL in men （ R ＝ 0.480, P ＜ 0.0001 ） , but no correlation was seen in women. A strong negative correlation between BAP test results and TG was also noted in men （ R ＝ 0.571, P ＜ 0.0001 ） , and a moderate negative correlation was detected in women （ R ＝ 0.344, P ＝ 0.0133 ） . A positive correlation between d-ROMs tests and OxLDL was seen in women under 50 years of age （ R ＝ 0.399, P ＝ 0.0393 ） , but this correlation was not present in women who were 50 years of age or older （ R ＝ 0.00656, P ＝ 0.976 ） . Correlations between oxidative stress and OxLDL and between antioxidant potential and TG in men were more prominent than in women. This finding suggests that decreasing oxidative stress in the blood to prevent atherosclerosis is more important for men.","PeriodicalId":23019,"journal":{"name":"The Showa University Journal of Medical Sciences","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91280362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Nagatomo, T. Shirakura, K. Fukuchi, Takahiro Takuma, I. Tokimatsu, Y. Niki
{"title":"Monitoring Quinolone Resistance Due to Mutations in GyrA and ParC in Haemophilus Influenzae（2012-17）","authors":"Y. Nagatomo, T. Shirakura, K. Fukuchi, Takahiro Takuma, I. Tokimatsu, Y. Niki","doi":"10.15369/sujms.32.81","DOIUrl":"https://doi.org/10.15369/sujms.32.81","url":null,"abstract":"","PeriodicalId":23019,"journal":{"name":"The Showa University Journal of Medical Sciences","volume":"15 2","pages":"81-90"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91471696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Nomura, Y. Akutsu, H. Tsujita, S. Kondo, T. Sekimoto, S. Sato, Hideaki Tanaka, K. Arai, Y. Oishi, K. Ogura, S. Tsukamoto, Toshihiko Gokan, Hiroki Tanisawa, K. Kaneko, Yusuke Kodama, Hidenari Matsumoto, T. Shinke
This study aimed to clarify whether native coronary artery(CA) calci cation before index percutaneous coronary intervention(PCI) has an impact on the effectiveness of drug-coated balloon(DCB) angioplasty for the treatment of instent restenosis(ISR). 100 consecutive patients with 166 ISR lesions underwent quantitative coronary angiography(QCA) before and after index PCI and before and after DCB angioplasty for ISR. CA calci cation before index PCI was assessed by angiography and results were analyzed to reveal the predictive values for target lesion revascularization(TLR) and major adverse cardiac events(MACE). During 1.03±1.03 years of follow-up, TLR occurred in 44 lesions(26.5%) and MACE in 33 patients(33%). On multivariate analysis, CA calci cation before index PCI (p=0.016), and % diameter of stenosis(%DS)≥73%(p=0.023) and minimal lumen diameter(MLD)<0.65 mm(p=0.001) before DCB angioplasty were independent predictors for TLR after DCB angioplasty. MACE was also associated with CA calci cation before index PCI(p=0.01), and %DS ≥ 73%(p=0.001) and MLD<0.65 mm(p=0.01) before DCB angioplasty, but only %DS≥73% before DCB angioplasty was an independent predictor for MACE after DCB angioplasty (p=0.039). The combination of CA calci cation before index PCI and these QCA factors before DCB angioplasty was an independent and more powerful predictor for MACE than the QCA factors alone(p<0.001). Thereafter, the combination of CA calci cation and %DS ≥ 73% before DCB angioplasty strati ed the risk of MACE after DCB angioplasty(p<0.05). CA calcification before index PCI, as well as anatomical information at ISR, have an impact on outcome after DCB angioplasty for ISR.
{"title":"Impact of Native Coronary Artery Calcification on Lesion Outcome Following Drug-Coated Balloon Angioplasty for Treatment of In-Stent Restenosis","authors":"K. Nomura, Y. Akutsu, H. Tsujita, S. Kondo, T. Sekimoto, S. Sato, Hideaki Tanaka, K. Arai, Y. Oishi, K. Ogura, S. Tsukamoto, Toshihiko Gokan, Hiroki Tanisawa, K. Kaneko, Yusuke Kodama, Hidenari Matsumoto, T. Shinke","doi":"10.15369/sujms.32.57","DOIUrl":"https://doi.org/10.15369/sujms.32.57","url":null,"abstract":"This study aimed to clarify whether native coronary artery(CA) calci cation before index percutaneous coronary intervention(PCI) has an impact on the effectiveness of drug-coated balloon(DCB) angioplasty for the treatment of instent restenosis(ISR). 100 consecutive patients with 166 ISR lesions underwent quantitative coronary angiography(QCA) before and after index PCI and before and after DCB angioplasty for ISR. CA calci cation before index PCI was assessed by angiography and results were analyzed to reveal the predictive values for target lesion revascularization(TLR) and major adverse cardiac events(MACE). During 1.03±1.03 years of follow-up, TLR occurred in 44 lesions(26.5%) and MACE in 33 patients(33%). On multivariate analysis, CA calci cation before index PCI (p=0.016), and % diameter of stenosis(%DS)≥73%(p=0.023) and minimal lumen diameter(MLD)<0.65 mm(p=0.001) before DCB angioplasty were independent predictors for TLR after DCB angioplasty. MACE was also associated with CA calci cation before index PCI(p=0.01), and %DS ≥ 73%(p=0.001) and MLD<0.65 mm(p=0.01) before DCB angioplasty, but only %DS≥73% before DCB angioplasty was an independent predictor for MACE after DCB angioplasty (p=0.039). The combination of CA calci cation before index PCI and these QCA factors before DCB angioplasty was an independent and more powerful predictor for MACE than the QCA factors alone(p<0.001). Thereafter, the combination of CA calci cation and %DS ≥ 73% before DCB angioplasty strati ed the risk of MACE after DCB angioplasty(p<0.05). CA calcification before index PCI, as well as anatomical information at ISR, have an impact on outcome after DCB angioplasty for ISR.","PeriodicalId":23019,"journal":{"name":"The Showa University Journal of Medical Sciences","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82950511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Errata for THE SHOWA UNIVERSITY JOURNAL OF MEDICAL SCIENCES","authors":"","doi":"10.15369/sujms.32.113","DOIUrl":"https://doi.org/10.15369/sujms.32.113","url":null,"abstract":"","PeriodicalId":23019,"journal":{"name":"The Showa University Journal of Medical Sciences","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80692822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Takeyama, Toshi Hashimoto, Akio Kotake, Y. Hori, Y. Tashiro, Takaki Hayashi, Kota Watanabe, T. Isobe, Tomoko Norose, N. Ohike
: This study investigated retrospectively the diagnostic yield and complication rate of transthoracic needle biopsies for posterior thoracic pulmonary lesions using C-arm cone-beam computed tomography （ CBCT ） . The risk factors for pulmonary hemorrhage were evaluated. Our study included 113 patients with 113 posterior pulmonary lesions （ mean longest diameter: 30.6 mm, and mean depth: 4.7 mm ） through the erector spinal muscles using a 19 / 20-gauge coaxial system. The diagnostic performances of procedures for malignant lesions and the incidence of complications after biopsies were also assessed. The patient-related and procedure-related variables were investigated. Risk factors for pulmonary hemorrhage were analyzed with a multivariate logistic regression analysis. Findings revealed 99 malignant, 13 benign, and one intermediate lesion. Sensitivity, specificity, and diagnostic accuracy rates were 100 ％ （ 99 / 99 ） , 92.3 ％ （ 12 / 13 ） , and 99.1 ％ （ 111 / 112 ） , respectively. Air emboliza-tion, hemothorax, hemoptysis, pneumothorax, and pulmonary hemorrhage, occurred in 0, 2, 12, 48, and 70 procedures. The averaged spinous process-pleura depth and the traversed lung parenchyma depth achieved by the introducer needles were 54.2 mm and 27.4 mm, respectively. The needle at the pleural puncture site within the intercostal space was in middle （ 31 ％） and inferior （ 69 ％） areas. The of hemorrhage in smaller lesions （ p ＝ 0.001 ） . Manual evacuation was performed in five procedures for patients with pneumothorax. The chest tube placement （ trocar ＞ 8 Fr ） was performed in two procedures in patients with hemothorax and pneumothorax. In conclusion, the biopsy method with a posterior intercostal approach for posterior thoracic pulmonary lesions yielded high diagnostic accuracy and few major complications.
{"title":"C-arm Cone-beam CT-guided Needle Biopsies through the Erector Spinal Muscle for Posterior Thoracic Pulmonary Lesions","authors":"N. Takeyama, Toshi Hashimoto, Akio Kotake, Y. Hori, Y. Tashiro, Takaki Hayashi, Kota Watanabe, T. Isobe, Tomoko Norose, N. Ohike","doi":"10.15369/sujms.32.193","DOIUrl":"https://doi.org/10.15369/sujms.32.193","url":null,"abstract":": This study investigated retrospectively the diagnostic yield and complication rate of transthoracic needle biopsies for posterior thoracic pulmonary lesions using C-arm cone-beam computed tomography （ CBCT ） . The risk factors for pulmonary hemorrhage were evaluated. Our study included 113 patients with 113 posterior pulmonary lesions （ mean longest diameter: 30.6 mm, and mean depth: 4.7 mm ） through the erector spinal muscles using a 19 / 20-gauge coaxial system. The diagnostic performances of procedures for malignant lesions and the incidence of complications after biopsies were also assessed. The patient-related and procedure-related variables were investigated. Risk factors for pulmonary hemorrhage were analyzed with a multivariate logistic regression analysis. Findings revealed 99 malignant, 13 benign, and one intermediate lesion. Sensitivity, specificity, and diagnostic accuracy rates were 100 ％ （ 99 / 99 ） , 92.3 ％ （ 12 / 13 ） , and 99.1 ％ （ 111 / 112 ） , respectively. Air emboliza-tion, hemothorax, hemoptysis, pneumothorax, and pulmonary hemorrhage, occurred in 0, 2, 12, 48, and 70 procedures. The averaged spinous process-pleura depth and the traversed lung parenchyma depth achieved by the introducer needles were 54.2 mm and 27.4 mm, respectively. The needle at the pleural puncture site within the intercostal space was in middle （ 31 ％） and inferior （ 69 ％） areas. The of hemorrhage in smaller lesions （ p ＝ 0.001 ） . Manual evacuation was performed in five procedures for patients with pneumothorax. The chest tube placement （ trocar ＞ 8 Fr ） was performed in two procedures in patients with hemothorax and pneumothorax. In conclusion, the biopsy method with a posterior intercostal approach for posterior thoracic pulmonary lesions yielded high diagnostic accuracy and few major complications.","PeriodicalId":23019,"journal":{"name":"The Showa University Journal of Medical Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89613072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Nishii, K. Seo, Aleksander Tatsuya Izdebski, M. Kushima, R. Takagi, S. Yoshizawa, S. Umemura, K. Ichizuka, A. Sekizawa
: High-intensity focused ultrasound （ HIFU ） can non-invasively irradiate inside the body. However, when used to treat fetuses, it can cause thermal burns of the mother ʼ s abdominal wall at the skin interface. This study was carried out to determine whether a modified HIFU transducer enabling split-aperture irradiation can prevent thermal burns. Two HIFU transducers were compared: a conventional transducer using full-aperture irradiation and a modified transducer using split-aperture irradiation. The modified transducer was divided into six sectors for split-aperture irradiation and had a larger surface area and a smaller F number （ focal length / aperture diameter ） than the conventional transducer. HIFU was delivered to eight sites on the left and right leg of a three-month-old baby pig under general anesthesia, and the sites were assessed for thermal burning by two or more derma-tologists. The same person performed all irradiations. Full-aperture irradiation with the conventional transducer caused deep dermal burns at all target sites, while split-aperture irradiation with the modified transducer caused only epidermal burns or superficial dermal burns. Split-aperture irradiation using a modified HIFU transducer with six sectors and a smaller F number reduces the severity of skin burns, and thus will improve the safety of HIFU therapy.
{"title":"Development of a High-intensity Focused Ultrasound Exposure Device for Reducing Skin Burn Risk","authors":"S. Nishii, K. Seo, Aleksander Tatsuya Izdebski, M. Kushima, R. Takagi, S. Yoshizawa, S. Umemura, K. Ichizuka, A. Sekizawa","doi":"10.15369/sujms.32.1","DOIUrl":"https://doi.org/10.15369/sujms.32.1","url":null,"abstract":": High-intensity focused ultrasound （ HIFU ） can non-invasively irradiate inside the body. However, when used to treat fetuses, it can cause thermal burns of the mother ʼ s abdominal wall at the skin interface. This study was carried out to determine whether a modified HIFU transducer enabling split-aperture irradiation can prevent thermal burns. Two HIFU transducers were compared: a conventional transducer using full-aperture irradiation and a modified transducer using split-aperture irradiation. The modified transducer was divided into six sectors for split-aperture irradiation and had a larger surface area and a smaller F number （ focal length / aperture diameter ） than the conventional transducer. HIFU was delivered to eight sites on the left and right leg of a three-month-old baby pig under general anesthesia, and the sites were assessed for thermal burning by two or more derma-tologists. The same person performed all irradiations. Full-aperture irradiation with the conventional transducer caused deep dermal burns at all target sites, while split-aperture irradiation with the modified transducer caused only epidermal burns or superficial dermal burns. Split-aperture irradiation using a modified HIFU transducer with six sectors and a smaller F number reduces the severity of skin burns, and thus will improve the safety of HIFU therapy.","PeriodicalId":23019,"journal":{"name":"The Showa University Journal of Medical Sciences","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81004458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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