Tissue engineering. Part C, Methods最新文献

Colorectal organoids, which accurately replicate the structure and function of the human colorectal epithelium, have become a valuable platform in a broad spectrum of fundamental biological research and clinical applications. This study employs bibliometric analysis to develop a knowledge domain map specifically focusing on colorectal organoid research. Articles were sourced from the Web of Science Core Collection, and CiteSpace 6.3.R1 was utilized to analyze the literature, including outputs, journals, countries, institutions, authors, cocited authors, references, co-occurring terms, and burst terms. Subsequently, we examined prevailing research themes and focal points and identified potential future research directions within this domain. Between 2010 and 2025, a total of 719 articles related to colorectal organoid research were published. Among these, the journal Nature Communications published the highest number of papers. The United States and Utrecht University were identified as the most prolific country and institution, respectively. Hans Clevers emerged as the most prolific author, while Toshiro Sato had the highest number of cocitations, indicating that both are ideal candidates for academic collaboration. The research focus on colorectal organoids has evolved from basic biological characteristics to disease modeling and clinical applications, and further towards an in-depth exploration of functional mechanisms and precision medicines. The terms "patient-derived organoids", "disease modeling", "epithelial barrier", and "personalized medicine" have garnered significant attention between 2020 and 2025, highlighting them as promising areas for future research. Research on colorectal organoids has achieved substantial progress, positioning itself as a vital interdisciplinary field that integrates fundamental biology with clinical medicine. Future studies should focus on optimizing organoid culture methodologies, exploring functional mechanisms, and expanding clinical applications-especially in disease modeling and personalized medicine.

Adipose tissue is an abundant and clinically accessible source of stromal cells. Stromal vascular fraction (SVF) and nanofat have been widely investigated for their regenerative potential; however, commercial systems vary considerably in yield, viability, and regulatory oversight. Most devices report fresh results only, with limited validation following cryopreservation. Mesenchymal stromal cells derived from adipose tissue have also attracted attention due to their accessibility, immunomodulatory effects, and multipotent differentiation capacity. Uvence has developed a proprietary workflow for adipose tissue processing that integrates washing, cryopreservation, thawing, and emulsification within a Human Tissue Authority-regulated laboratory. The process includes Good Manufacturing Practices (GMP) Annex 1-aligned environmental monitoring and independent quality control (QC) testing. Critically, this workflow validates postthaw cell viability, addressing a gap in current SVF/nanofat approaches. Three cryopreserved donor samples demonstrated a mean postthaw viability of ∼91% (range 90.5-92%), consistently exceeding the International Federation for Adipose Therapeutics and Science (IFATS)/ International Society for Cell and Gene Therapy (ISCT) 70% threshold. Benchmarking against global systems showed Uvence postthaw viability to be equivalent to or higher than fresh outcomes reported for enzymatic platforms (Celution, 85-91%; InGeneron, 86%) and mechanical platforms (Lipocube, Tulip, ∼96%). Unlike competitor devices, Uvence has validated freeze-thaw performance, providing a stable and compliant platform. This study also presents in vitro culture and characterization of stromal cells expanded from Uvence nanofat-derived SVF samples, including flow cytometry, morphology, and trilineage differentiation. Flow cytometry confirmed high expression of CD73, CD90, and CD105, with minimal expression of CD34/CD45, consistent with the ISCT criteria. While these findings are limited to research characterization and do not constitute approval for therapeutic use, they demonstrate that the Uvence workflow delivers a quality-focused approach to adipose tissue processing.

Background & objective: Endoscopic Submucosal Dissection (ESD) effectively treats early gastric cancer, but postoperative complications limit its clinical use. Therefore, this study examines how esophageal mucosal wound protective gels improve wound healing and reduce post-ESD complications.

Methods: The gels were characterized for physical properties and stability using rheological behavior, injectability, swelling capacity, and enzymatic degradation resistance. Biocompatibility was assessed via hemolysis testing, cytotoxicity assays, and oral mucosal irritation tests. Furthermore, wound repair potential was evaluated through cell proliferation, migration, and cell cycle analysis in Het-1A cells. Finally, in vivo recovery experiments were conducted to assess post-ESD wound healing efficacy.

Results: The gels exhibited favorable physical properties, chemical stability, and biocompatibility. Specifically, they maintained stability in the digestive tract, underwent rapid gelation at 37°C, and promoted cell proliferation. Post-ESD evaluation further revealed improved mucosal healing with no significant bleeding events.

Conclusion: The developed esophageal mucosal wound-protective gels fulfill the requirements for submucosal interventions and show promising potential for ESD wound repair via rapid in situ gelation. This platform could be adapted for various endoscopic procedures and provides new insights for digestive tract tissue engineering applications.

Background: The demand for effective vascular grafts continues to increase with the increasing prevalence of cardiovascular disease. The decellularized human umbilical vein (HUV) offers a promising scaffold for cellular removal, extracellular matrix (ECM) preservation, and balanced biocompatibility.

Objective: To evaluate the efficacy of a sodium dodecyl sulfate (SDS)-based decellularization protocol for HUV preparation intended for future stem cell seeding.

Methods: HUVs were obtained from four full-term cesarean deliveries (37-39 weeks; total length 210 cm) and assigned to a control group or five SDS-treated groups (0.5% SDS for 6, 12, and 24 h; 1% SDS for 12 and 24 h). Following decellularization, scaffolds were freeze-dried, gamma-sterilized, and analyzed for DNA content, cell viability (MTT assay), collagen/elastin retention, and histological and ultrastructural changes.

Results: The 0.5% SDS for 6 h group achieved optimal results, with significant cell clearance (1.82 ± 1.75 vs. 5.55 ± 2.49 cells/field; p = 0.009) and DNA reduction (59.6 ± 22.62 vs. 258.09 ± 107.63 ng/µL), while maintaining cell viability (87.05 ± 17.14% vs. 84.79 ± 14.3%; p = 0.781). Collagen (20.80 ± 5.26% vs. 27.34 ± 5.34%; p = 0.152) and elastin (21.13 ± 5.02% vs. 24.47 ± 8.01%; p = 0.437) retention were comparable to controls.

Conclusions: A 0.5% SDS 6 h decellularization protocol effectively removed cellular components while preserving ECM integrity and biocompatibility, offering a balanced and feasible approach for preparing HUV scaffolds for vascular tissue engineering applications.

Bioprinting continues to expand as a preferred fabrication technology as new biomaterials emerge. These biomaterials must be biocompatible, in addition to exhibiting suitable printing characteristics. Printability is usually gauged by metrics such as extrudability, fiber morphology, and shape fidelity, and optimized via design of experiments or machine learning methods. However, these optimization techniques often overlook dynamic variables such as time-dependent behavior and batch-to-batch variability that can significantly affect the quality of printed structures. In this study, we 3D-printed colloidal hydrogels to investigate the effects of printing parameters and elapsed time on overall hydrogel print quality.

This study used bibliometric analysis to comprehensively map the research landscape of immunomodulatory biomaterials enhancing implant osseointegration between 2005 and 2025. Publications retrieved from the Web of Science Core Collection were analyzed to identify temporal trends, contributing countries, institutions, journals, cocitation networks, and keyword evolution. A total of 419 articles were identified. The annual output and citations increased steadily, with China, the United States, and Australia as major contributors. The terms '3D printing', 'scaffolds', and 'macrophage polarization' emerged as recent hotspots, reflecting a shift from mechanistic exploration to clinical translation. Compared with prior reviews focusing mainly on mechanisms, this study also analyzed the depth of international collaboration, clinical orientation of journals, and bottlenecks in translational application. The field has evolved from theoretical construction (osteoimmunomodulation theory) to technological innovation (nanoengineering, dynamic response design). Future work should integrate intelligent responsive biomaterials with multiomics validation to accelerate the transition from passive repair to active regulation in bone regeneration.

Bone and cartilage injuries are highly prevalent and arise from diverse pathogenic mechanisms, placing a substantial burden on patients' health, quality of life, and on families and society. Piezoelectric materials, inspired by tissue engineering concepts and the intrinsic piezoelectricity of human tissues, can harness the physiological electrical microenvironment to enhance tissue regeneration. To better understand the development of this field, we employed data from the Web of Science Core Citation (WoSCC) database as the core and primary focus for conducting bibliometric research and applied tools including Bibliometrix, Origin, Python, CiteSpace, and VOSviewer. A total of 388 publications from 46 countries were identified, with China, the United States, and Iran being the leading contributors. Fangwei Qi had the highest publication output, while C. Ribeiro had the highest cocitation frequency. The most productive institutions were Shanghai Jiao Tong University, the Fourth Military Medical University, and the University of Chinese Academy of Sciences. ACS Applied Materials & Interfaces published the largest number of articles. The most frequent keywords included "bone regeneration," "osteogenic differentiation," "piezoelectric," "scaffolds," and "hydroxyapatite." Furthermore, we employed Scopus as a validation database to cross-verify the publication trends and keyword hotspots derived from WoSCC, with the results demonstrating a high degree of consistency. These findings reveal that research on the role of piezoelectric materials in bone and cartilage regeneration is expanding rapidly, highlighting the current hotspots and emerging trends and providing valuable insights to guide future studies in this area.

Over the past four decades, calcium phosphate cements (CPCs) have emerged as promising materials for bone repair due to their biocompatibility, osteoconductivity, and in situ hardening properties. Developed from a combination of calcium phosphate (CaP)-based powder and a liquid phase, CPCs undergo a chemical reaction when mixed, forming a crystalline solid structure at body temperature. This hardening process is characterized by its mildly exothermic reaction, offering significant advantages compared with cements like polymethylmethacrylate, commonly used in orthopedic surgeries. Over more than four decades of research, various modifications have been introduced to the physical, mechanical, and biological properties of CPCs, making them adaptable to a wide range of clinical applications, from craniofacial surgery to bone tissue engineering and drug delivery systems. Despite all the advancements, the widespread clinical use of CPCs still faces significant challenges, such as limitations in mechanical strength, degradation rate, and osteoinductive properties. This article provides a comprehensive historical and technical overview of CPC development from their initial discovery in 1980s to current innovations in formulation, physicochemical, mechanical, and biological properties. It highlights crucial milestones in each decade, covering the evolution of these cements and presenting the challenges that remain until today.

Arthrofibrosis is a common complication following total knee arthroplasty and is the result of a dysregulated immuno-inflammatory cascade, which culminates in excessive scar deposition by myofibroblasts within the knee joint. Pharmacotherapies for arthrofibrosis are limited, with treatment reliant on manipulation under anesthesia or lysis of adhesions with or without component revision. Bupivacaine (a local anesthetic) and meloxicam (a nonsteroidal anti-inflammatory drug) combination therapy may exert antifibrotic properties by limiting inflammatory cell recruitment and the subsequent release of profibrotic cytokines. The purpose of this study was to evaluate the effects of this combination therapy in a rabbit model of arthrofibrosis by evaluating live and postsacrifice knee biomechanics and gene expression of posterior knee capsule tissue. Forty New Zealand white rabbits were equally divided into two experimental groups and prospectively studied to assess knee passive extension angles (PEA), terminal posterior capsular stiffness, and the inflammatory milieu of the posterior knee joint capsule. Experimental limbs with and without combination therapy showed similar live PEAs and terminal posterior capsular stiffness, with no significant differences between the groups. Gene expression analysis, however, revealed significant reduction in inflammatory genes but not profibrotic genes. In summary, bupivacaine and meloxicam combination therapy did not exert antifibrotic effects in a rabbit model of arthrofibrosis but significantly reduced the expression of inflammatory markers in the local environment of the posterior capsule.