Tissue & cell最新文献_第3页

Morphological characteristics of the lung in Eurasian teal (Anas crecca): Insights from cross-sectional, casting, histological, and SEM analyses 欧亚绿鸭肺的形态特征：来自横断面、铸型、组织学和扫描电镜分析的见解

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell

Pub Date : 2026-01-26 DOI: 10.1016/j.tice.2026.103353

Alaa Abd El Moneam , Ashraf El Sharaby , Asmaa Aboelnour , Mohamed M.A. Abumandour , Ahmed G. Nomir

This study detailed a comprehensive morphological characterization of the adult Eurasian teal (Anas crecca) lungs through anatomical techniques, including gross anatomy, cross-sectional analysis, corrosion casting, histology, and scanning electron microscopy (SEM). Twenty lungs were examined to identify their topographical position, structural organization, and microarchitecture. Gross anatomical examination showed trapezoidal, non-lobated lungs located craniodorsally within the thoracoabdominal cavity, showing distinct surfaces and borders with seven intercostal tori. Corrosion casting revealed the branching pattern of the bronchial system, highlighting the intrapulmonary primary bronchus (IPPB) and its secondary bronchi, including medioventral (MVSBs) and laterodorsal (LVSBs), interconnected by parabronchi (PB). Seven laterodorsal secondary bronchi (LDSBs) and four lateroventral secondary bronchi (LVSBs) were identified, with posterior secondary bronchi (POSBs) also present along the lateral wall of the IPPB. The POSBs were the shortest, while the medioventral secondary bronchi were the largest. SEM examination showed the spatial arrangement of elongated paleo- and hexagonal neo-parabronchi, characterized by hexagonal architecture and atrial openings leading to air capillaries. Histological examination confirmed the presence of pseudostratified ciliated epithelium in primary and secondary bronchi, smooth muscle layers, and reticular fiber-rich septa separating parabronchi. Two types of hexagonal parabronchi were identified: longitudinally arranged in the paleopulmonic region and circular in the neopulmonic region. The interatrial septa were lined with squamous to cuboidal epithelial cells. These findings enhance understanding of avian respiratory morphology and provide baseline data for comparative anatomical and functional studies in waterfowl species.

本研究通过解剖学技术，包括大体解剖、横断面分析、腐蚀铸造、组织学和扫描电子显微镜（SEM），详细描述了成年欧亚绿鸭（Anas crecca）肺部的全面形态学特征。检查了20个肺，以确定其地形位置，结构组织和微结构。大体解剖检查显示肺呈梯形，无叶状，位于胸腹腔内，呈颅朝上，有7个肋间环面。腐蚀铸型显示支气管系统的分支模式，突出显示肺内初级支气管（IPPB）及其次级支气管，包括中腹侧支气管（MVSBs）和侧腹侧支气管（LVSBs），由副支气管（PB）相互连接。在IPPB的侧壁上还发现了7条外侧背侧继发支气管（LDSBs）和4条外侧腹侧继发支气管（LVSBs），以及后侧继发支气管（POSBs）。posb最短，而中腹次支气管最大。扫描电镜检查显示长形古旁支气管和六边形新旁支气管的空间排列，以六边形结构和心房开口通向空气毛细血管为特征。组织学检查证实在原发性和继发性支气管、平滑肌层和分隔副支气管的网状纤维丰富的间隔中存在假层状纤毛上皮。发现了两种六边形副支气管：纵向分布在古肺脏区，圆形分布在新肺脏区。房间隔内排列有鳞状至立方状上皮细胞。这些发现增强了对鸟类呼吸形态的认识，并为水禽物种的比较解剖学和功能研究提供了基线数据。

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引用次数: 0

Comparing mesenchymal stem/stromal cells from dermis discarded in elective surgeries: Identifying optimal sources for regenerative medicine 比较选择性手术中丢弃的真皮间充质干细胞/基质细胞：确定再生医学的最佳来源

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell

Pub Date : 2026-01-26 DOI: 10.1016/j.tice.2026.103352

Helena Debiazi Zomer , Adriane Cristina Fagundes , Lais Andrade Ferreira , Augusto Cesar Spadaccia Asciutti , Priscilla Barros-Delben , Maiara Marques da Silva , Julia Mitze , Talita da Silva Jeremias , Andrea Gonçalves Trentin

The dermis is a valuable and accessible source of mesenchymal stem/stromal cells (MSCs), particularly for applications in skin wound healing. Dermal MSCs (DSCs) can be isolated from skin fragments typically discarded during routine elective surgeries, such as facelifts (rhytidectomies), tummy tucks (abdominoplasties), and circumcisions (postectomies). While MSCs generally share core stem cell properties, their behavior may be influenced by factors such as donor age, sex, and the anatomical tissue origin. Understanding the specific characteristics of DSCs from different sources is therefore crucial for identifying the most suitable cells for clinical use. In this study, we compared human DSCs obtained from rhytidectomies (postmenopausal facial dermis), abdominoplasties (adult abdominal dermis), and postectomies (prepubertal foreskin dermis) by assessing their isolation efficiency, stemness markers, proliferative capacity, and in vitro wound healing. All three DSC populations exhibited comparable stemness profiles; however, DSCs from prepubertal foreskin demonstrated superior isolation yield, expansion potential, and colony-forming efficiency, likely reflecting the younger age of the tissue donors. Despite these differences, DSCs from both prepubertal foreskin and adult abdominal skin exhibited similar wound healing performance in vitro. In conclusion, our findings indicate that the source of dermal tissue significantly influences DSC behavior in vitro, with cells derived from postectomies and abdominoplasties showing enhanced potential compared to those from rhytidectomies.

真皮是间充质干细胞（MSCs）的重要来源，尤其适用于皮肤伤口愈合。真皮间质干细胞（DSCs）可以从常规选择性手术中通常丢弃的皮肤碎片中分离出来，如面部拉皮术（除皱术）、腹部整形术（腹部整形术）和包皮环切术（切除术后）。虽然间充质干细胞通常具有核心干细胞特性，但它们的行为可能受到供体年龄、性别和解剖组织来源等因素的影响。因此，了解不同来源的骨髓间充质干细胞的具体特征对于确定最适合临床使用的细胞至关重要。在这项研究中，我们比较了从除皱术（绝经后面部真皮）、腹部成形术（成人腹部真皮）和切除术后（青春期前包皮真皮）中获得的人dsc，评估了它们的分离效率、干性标志物、增殖能力和体外伤口愈合。所有3个DSC种群均表现出可比性；然而，来自青春期前包皮的dsc表现出更高的分离率、扩增潜力和集落形成效率，这可能反映了组织供体的年龄更小。尽管存在这些差异，来自青春期前包皮和成人腹部皮肤的dsc在体外表现出相似的伤口愈合性能。总之，我们的研究结果表明，真皮组织的来源显著影响体外DSC的行为，与来自除皱手术的细胞相比，来自切除后和腹部整形手术的细胞表现出更高的潜力。

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引用次数: 0

SURF4 inhibits autophagy to enhance lung adenocarcinoma progression and stemness through Hedgehog pathway regulation. SURF4通过调控Hedgehog通路抑制自噬，促进肺腺癌的进展和干性。

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell

Pub Date : 2026-01-26 DOI: 10.1016/j.tice.2026.103351

Junzheng Zhou, Shiwei Nie, Xiaodong Zheng, Xudong Wei, Jinrui Zhang, Xiaojuan Shen, Weimin Zhang

Background: Drug resistance, metastasis, and recurrence are major contributors to the poor survival rates of lung adenocarcinoma (LUAD) patients. Studies suggest that tumor stemness is a key driver of drug resistance, recurrence, and metastasis. Although Surfeit locus protein 4 (SURF4) has been shown to promote tumor cell stemness, the molecular mechanisms by which SURF4 regulates LUAD growth and stemness remain elusive.

Methods: Differentially expressed genes were identified using the TCGA-LUAD database to compare LUAD tissues with adjacent normal tissues, followed by experimental validation. qRT-PCR, CCK-8, and flow cytometry assays were used to assess the functional impact of SURF4. Tumor stemness was evaluated by sphere formation assays and Western blotting. In addition, GSEA combined with cellular experiments was employed to validate the mechanism underlying SURF4-mediated regulation of LUAD growth and stemness.

Results: There was abnormal upregulation of SURF4 in LUAD tissues and cells. Knockdown of SURF4 enhanced autophagy and suppressed LUAD cell proliferation and stemness. Mechanistically, SURF4 was found to activate the Hedgehog pathway by facilitating Sonic hedgehog (Shh) transport, thereby inhibiting autophagy. Rescue experiments confirmed that SURF4's effects on LUAD growth and stemness were primarily mediated through this mechanism.

Conclusion: SURF4 promotes LUAD proliferation and maintains tumor stemness by suppressing autophagy through Hedgehog pathway activation.

背景：耐药、转移和复发是肺腺癌（LUAD）患者生存率低的主要原因。研究表明，肿瘤干性是耐药、复发和转移的关键驱动因素。虽然过量位点蛋白4 （SURF4）已被证明可促进肿瘤细胞的干性，但SURF4调节LUAD生长和干性的分子机制尚不清楚。方法：利用TCGA-LUAD数据库鉴定差异表达基因，将LUAD组织与邻近正常组织进行比较，并进行实验验证。采用qRT-PCR、CCK-8和流式细胞术评估SURF4对功能的影响。用球形成法和免疫印迹法评价肿瘤干性。此外，GSEA结合细胞实验验证了surf4介导的LUAD生长和干性调节的机制。结果：LUAD组织细胞中SURF4表达异常上调。下调SURF4可增强自噬，抑制LUAD细胞的增殖和干性。在机制上，SURF4被发现通过促进Sonic Hedgehog （Shh）运输来激活Hedgehog通路，从而抑制自噬。救援实验证实SURF4对LUAD生长和干性的影响主要是通过这一机制介导的。结论：SURF4通过激活Hedgehog通路抑制自噬，促进LUAD增殖，维持肿瘤干性。

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引用次数: 0

PRMT1-rich exosomes derived from M2 macrophages as novel therapeutics for enhancing fracture healing 来自M2巨噬细胞的富含prmt1的外泌体作为促进骨折愈合的新疗法

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell

Pub Date : 2026-01-24 DOI: 10.1016/j.tice.2026.103348

Haifeng Hong , Cuiyun Zhou , Haibing Wang , Changhui Lin , Min Fang , Juntian Liu , Minhui Yang

Purpose

sM2 macrophage-derived exosomes (M2-Exo) have emerged as promising mediators of bone repair due to their anti-inflammatory and pro-regenerative properties. However, the molecular mechanisms underlying the interaction between M2-Exo and bone marrow mesenchymal stem cells (BMSCs) are awaiting clarification. Our previous study found that protein arginine methyltransferase 1 (PRMT1) in fracture-derived exosomes promotes bone healing. This study aimed to determine whether PRMT1-containing M2-Exo exerts an osteoprotective effect during fracture healing and elucidate its underlying mechanisms.

Methods

Following knockdown of PRMT1 in M2-Exo and then treating BMSCs, osteogenic differentiation ability of BMSCs was evaluated by qRT-PCR, western blot, Alizarin Red S, and Alkaline phosphatase (ALP). Immunoprecipitation was employed to clarify regulatory effect of PRMT1 on Src-associated in mitosis 68 kDa (Sam68) and its asymmetric dimethylarginine (ADMA) modification.

Results

PRMT1 was overexpressed in M2-Exo extracted from serum samples of fracture rats. M2-Exo delivered PRMT1 to BMSCs. PRMT1 knockdown in M2-Exo impaired osteogenic differentiation, as evidenced by suppressing mineral deposition and ALP activity (42.3 % and 60.8 % reduction vs. sh-NC; p < 0.01), with a decrease in expression of Bone Morphogenetic Protein 2 (BMP2) and RUNX Family Transcription Factor 2 (RUNX2) (74.9 % and 61.8 % decline vs. sh-NC; p < 0.01). PRMT1 interacted with Sam68, and its knockdown reduced ADMA modification and Sam68 protein expression. Sam68 overexpression partially rescued the osteogenic defects caused by PRMT1 knockdown, with increased mineralization level and ALP (44.3 % and 87.6 % increase vs. M2-Exo-sh-PRMT1 + oe-NC; p < 0.05), along with elevated BMP2 and RUNX2 expression (79.5 % and 98.8 % increase vs. M2-Exo-sh-PRMT1 + oe-NC; p < 0.01).

Conclusion

PRMT1 released by M2-Exo is delivered to BMSCs, which enhances the osteogenic differentiation by catalyzing Sam68 ADMA. These findings provide a mechanistic and preclinical rationale for the potential use of M2-Exo to improve bone healing.

m2巨噬细胞来源的外泌体（M2-Exo）由于其抗炎和促进再生的特性而成为骨修复的有前途的介质。然而，M2-Exo与骨髓间充质干细胞（BMSCs）相互作用的分子机制尚待阐明。我们之前的研究发现，骨折源性外泌体中的蛋白精氨酸甲基转移酶1 （PRMT1）促进骨愈合。本研究旨在确定含有prmt1的M2-Exo是否在骨折愈合过程中发挥骨保护作用并阐明其潜在机制。方法敲除M2-Exo中PRMT1后处理骨髓间充质干细胞，采用qRT-PCR、western blot、茜素红S、碱性磷酸酶（ALP）检测骨髓间充质干细胞的成骨分化能力。采用免疫沉淀法阐明PRMT1对src相关的有丝分裂68 kDa （Sam68）及其不对称二甲基精氨酸（ADMA）修饰的调控作用。结果sprmt1在骨折大鼠血清提取的M2-Exo中过表达。M2-Exo将PRMT1传递给BMSCs。PRMT1敲低在M2-Exo中会损害成骨分化，这可以通过抑制矿物质沉积和ALP活性得到证明（与sh-NC相比，分别降低42.3 %和60.8 %;p <； 0.01），同时骨形态发生蛋白2 （BMP2）和RUNX家族转录因子2 （RUNX2）的表达降低（与sh-NC相比，分别降低74.9 %和61.8 %;p <； 0.01）。PRMT1与Sam68相互作用，其敲低降低了ADMA修饰和Sam68蛋白的表达。Sam68超表达部分获救成骨的缺陷造成PRMT1击倒,与矿化水平和增加高山(44.3 % 87.6 %的增长与M2-Exo-sh-PRMT1 + oe-NC; p & lt; 0.05),高架BMP2和RUNX2表达式(79.5 % 98.8 %的增长与M2-Exo-sh-PRMT1 + oe-NC; p & lt; 0.01)。结论M2-Exo释放的prmt1可通过介导Sam68 ADMA，促进骨髓间充质干细胞成骨分化。这些发现为潜在使用M2-Exo促进骨愈合提供了机制和临床前理论依据。

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引用次数: 0

Iron-quercetin complex ameliorates chronic kidney disease via inhibiting the renal TGF-β1/Smad3/Egr1 axis-mediated kidney injury and fibrosis 铁槲皮素复合物通过抑制肾脏TGF-β1/Smad3/Egr1轴介导的肾损伤和纤维化改善慢性肾脏疾病。

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell

Pub Date : 2026-01-24 DOI: 10.1016/j.tice.2026.103349

Jianchun Li , Luna Zhang , Qianqian Li , Yuanxia Zou , Yufang Ni , Jiraporn Kantapan , Nathupakorn Dechsupa , Li Wang

Background

Chronic kidney disease (CKD) remains a significant global health burden due to the lack of effective interventions. Here, we evaluated whether the metal-flavonoid compound, Iron-Quercetin complex (IronQ) can ameliorate CKD and examine its effect on the TGF-β1/Smad3/Egr1 signaling cascade.

Methods

To assess the IronQ’s therapeutic efficacy in renal fibrosis, two well-recognized in vivo models were employed: the unilateral ureteral obstruction (UUO) model and the adenine (Ade)-elicited renal fibrosis systems. The efficacy of IronQ was assessed through comprehensive analyses of renal injury and histopathological changes. In addition, an in vitro system of TGF-β1-induced profibrotic response was developed using renal fibroblasts (NRK-49F) and renal tubular cells (TCMK1) to dissect the cellular signaling. Furthermore, to elucidate the potential mechanisms by which IronQ ameliorates renal fibrosis, a proteomic screen was conducted to delineate the changes in protein expression after IronQ intervention.

Results

IronQ significantly improved biochemical parameters of kidney injury, evidenced by a marked decrease in serum creatinine and blood urea nitrogen (BUN) levels. Consistently, IronQ treatment attenuated renal fibrosis, which was evidenced by a decreased expression of extracellular matrix (ECM) markers, including α-SMA, FN, and Col1a1, as evidenced by immunohistochemistry, Western blot, and qRT-PCR analyses. In agreement with the in vivo findings, IronQ intervention also significantly reduced the fibrotic response in both NRK-49F and TCMK-1 cell lines. Proteomic analysis further demonstrated that IronQ specifically downregulated the TGF-β1-stimulated upregulation of Egr1. Subsequent validation confirmed that the IronQ intervention significantly suppressed Egr1 expression and inhibited the phosphorylation of Smad3. Importantly, co-immunoprecipitation results revealed that IronQ intervention directly inhibited the physical interaction between Egr1 and Smad3.

Conclusion

Our research demonstrates that IronQ is a promising therapeutic candidate for CKD, offering a novel strategy to combat renal fibrosis via targeting the TGF-β1/Smad3/Egr1 cascade.

背景：由于缺乏有效的干预措施，慢性肾脏疾病（CKD）仍然是一个重大的全球健康负担。在这里，我们评估了金属类黄酮化合物铁-槲皮素复合物（IronQ）是否可以改善CKD，并研究了其对TGF-β1/Smad3/Egr1信号级联的影响。方法：采用单侧输尿管梗阻（UUO）模型和腺嘌呤（Ade）诱导肾纤维化两种常用的体内模型来评估IronQ对肾纤维化的治疗效果。通过对肾损伤和组织病理学变化的综合分析来评估IronQ的疗效。此外，我们利用肾成纤维细胞（NRK-49F）和肾小管细胞（TCMK1）建立了TGF-β1诱导的促纤维化反应的体外系统，解剖细胞信号传导。此外，为了阐明IronQ改善肾纤维化的潜在机制，研究人员进行了蛋白质组学筛选，以描述IronQ干预后蛋白质表达的变化。结果：IronQ能显著改善肾损伤的生化指标，显著降低血清肌酐和血尿素氮（BUN）水平。通过免疫组织化学、Western blot和qRT-PCR分析，IronQ治疗可以一致地减轻肾纤维化，这可以通过细胞外基质（ECM）标志物（包括α-SMA、FN和Col1a1）的表达降低来证明。与体内研究结果一致，IronQ干预也显著降低了NRK-49F和TCMK-1细胞系的纤维化反应。蛋白质组学分析进一步证实IronQ特异性下调TGF-β1刺激的Egr1上调。随后的验证证实，IronQ干预显著抑制了Egr1的表达，抑制了Smad3的磷酸化。重要的是，共免疫沉淀结果显示，IronQ干预直接抑制了Egr1和Smad3之间的物理相互作用。结论：我们的研究表明IronQ是一种有前景的CKD治疗候选药物，通过靶向TGF-β1/Smad3/Egr1级联提供了一种对抗肾纤维化的新策略。

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引用次数: 0

Integrated Morphological Analysis Of The Small Intestine In The Pekin Duck's (Anas platyrhynchos) Using Gross Anatomical, Histological, Histochemical, and Scanning Electron Microscopic Techniques 利用大体解剖、组织学、组织化学和扫描电镜技术对北京鸭小肠进行综合形态学分析

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell

Pub Date : 2026-01-24 DOI: 10.1016/j.tice.2026.103346

Youssef Elsabbgh , Ashraf El Sharaby , Asmaa Aboelnour , Mohamed M.A. Abumandour , Ahmed G. Nomir

The adult White Pekin duck’s small intestine is anatomically adapted for rapid growth, high-fat diet digestion, and strong immunity, essential for its physiological needs. This study analyzes the adaptive anatomical features of the small intestine—duodenum, jejunum, and ileum—through gross examination, light microscopy (LM), histochemical techniques, scanning electron microscopy (SEM), and morphometric analysis. To achieve this objective, sixteen ducks were examined. Gross examination revealed a U-shaped duodenum, a coiled jejunum with Meckel’s diverticulum, and a straight ileum attached to paired ceca. Histologically, the duodenum displayed finger-like villi with the deepest crypts, while the jejunum had the tallest villi and the highest villus/crypt ratio. The ileum showed compact villi and the highest goblet cell (GC) density. GCs were quantified and differentiated using PAS, AB, and PAS-AB combination staining. PAS-positive (neutral mucins) cells predominated in villi, especially in the jejunum, while AB-positive (acidic mucins) cells were most abundant in ileal crypts, indicating regional specialization in mucosal protection. SEM revealed ultrastructural features including dense microvilli, epithelial exfoliation, and GC secretory vesicles. Enterocytes exhibited organelles such as mitochondria and rough endoplasmic reticulum, as well as junctional complexes including tight junctions and desmosomes. These findings highlight the segmental specialization of the Pekin duck’s small intestine, with the jejunum optimized for nutrient absorption and the ileum for immune defense. The detailed mucin profile and ultrastructural features provide insight into the digestive efficiency and adaptive physiology of this fast-growing avian species.

成年北京白鸭的小肠在解剖学上适合快速生长，消化高脂肪饲料，具有较强的免疫力，是其生理需要所必需的。本研究通过大体检查、光镜、组织化学技术、扫描电镜和形态计量学分析，分析小肠（十二指肠、空肠和回肠）的适应性解剖特征。为了达到这个目的，对16只鸭子进行了检查。大体检查显示u型十二指肠，螺旋状空肠伴梅克尔憩室，直回肠与成对盲肠相连。组织学上，十二指肠显示指状绒毛，隐窝最深，空肠绒毛最高，绒毛/隐窝比最高。回肠绒毛致密，杯状细胞（GC）密度最高。采用PAS、AB和PAS-AB联合染色对gc进行定量和分化。pas阳性（中性粘蛋白）细胞主要分布在绒毛中，特别是空肠，而ab阳性（酸性粘蛋白）细胞在回肠隐窝中最为丰富，表明粘膜保护具有区域特异性。扫描电镜显示其超微结构包括致密的微绒毛、上皮脱落和GC分泌囊泡。肠细胞具有细胞器，如线粒体和粗内质网，以及连接复合物，包括紧密连接和桥粒。这些发现强调了北京鸭小肠的节段特化，其中空肠用于营养吸收，回肠用于免疫防御。详细的粘蛋白轮廓和超微结构特征提供了对这种快速生长的鸟类的消化效率和适应性生理的深入了解。

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引用次数: 0

A preliminary model of an oral dysplastic lesion on a chip 口腔发育不良病变芯片的初步模型

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell

Pub Date : 2026-01-24 DOI: 10.1016/j.tice.2026.103347

Roberto Plebani , Tania Vanessa Pierfelice , Emira D’Amico , Mario Romano , Simonetta D’Ercole , Giovanna Iezzi , Morena Petrini

In vitro models of oral dysplasia fail to recapitulate physiologically relevant tissue-tissue interfaces and other microenvironmental cues. This study aimed to present a preliminary organ-on-a-chip (OoC) model of a precancerous oral cavity lesion (OD-OoC). The objective was to reproduce in a two-channel microfluidic device an in vitro tridimensional (3D) model characterized by an organized interaction between endothelial cells, fibroblasts, and dysplastic oral keratinocytes on a collagen I-coated membrane. On day 1, human umbilical vein endothelial cells (ECs) were introduced in the bottom channel, and the chip was inverted to allow cell adhesion to the lower side of the membrane. The chip was then inverted back to the original position, and human gingival fibroblasts (hGFs) were introduced into the top channel. On day 2, the laminar flow was activated, while uniform layers of hGFs and ECs were forming in the respective channels. On day 3, dysplastic oral keratinocytes (DOKs) were inoculated in the top channel above the hGFs layer. On day 5, the chip was fixed with 4 % paraformaldehyde and stained with antibodies targeting podoplanin, Trop2, and VE-cadherin for staining of hGFS, ECs, and DOKs, respectively. Confocal microscopy confirmed the presence of all cell types, showing fibroblast migration from the top channel to the bottom channel of the chip, where they localized between the membrane and the ECs. DOKs confined to the top channel showed slight and uneven E-cadherin and EpCAM (Epithelial Cell Adhesion Molecule) positivity, but evident positivity for Trop-2, confirming that their phenotype differed from that of healthy epithelial cells. The presented OD-OoC could enable in vitro monitoring of epithelial cell phenotype changes and cell migration across the membrane, suggesting its potential applicability in future oral cancer research.

口腔发育不良的体外模型不能概括生理上相关的组织-组织界面和其他微环境线索。本研究旨在建立一种初步的口腔癌前病变（OD-OoC）器官芯片（OoC）模型。目的是在双通道微流控装置中再现一个体外三维（3D）模型，其特征是内皮细胞、成纤维细胞和发育不良的口腔角化细胞在胶原i涂层膜上有组织的相互作用。第1天，将人脐静脉内皮细胞（ECs）引入底部通道，并将芯片倒置，使细胞粘附在膜的下部。然后将芯片倒转回原始位置，将人牙龈成纤维细胞（hGFs）引入顶部通道。在第2天，层流被激活，hgf和ec在各自的通道中形成了均匀的层。第3天，在hGFs层上方的通道中接种发育不良的口腔角化细胞（DOKs）。第5天，用4 %多聚甲醛固定芯片，用靶向podoplanin、Trop2和VE-cadherin的抗体进行染色，分别对hGFS、ECs和DOKs进行染色。共聚焦显微镜证实了所有细胞类型的存在，显示成纤维细胞从芯片的顶部通道迁移到底部通道，在那里它们定位在膜和内皮细胞之间。限制在顶部通道的DOKs表现出轻微且不均匀的E-cadherin和EpCAM（上皮细胞粘附分子）阳性，但Trop-2明显阳性，证实其表型与健康上皮细胞不同。该OD-OoC能够在体外监测上皮细胞表型变化和细胞跨膜迁移，提示其在未来口腔癌研究中的潜在适用性。

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引用次数: 0

Morphological architecture of the developing pancreas in quail embryos: A histological and ultrastructural perspective 鹌鹑胚胎中发育中的胰腺的形态结构：组织学和超微结构的观点。

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell

Pub Date : 2026-01-23 DOI: 10.1016/j.tice.2026.103344

Fatma Abdelhakeem , Elsayed S.I. Mohammed , Mohammed Al-Rasheed , Fatma A. Madkour

Organogenesis of the avian pancreas is a critical developmental process that ensures proper digestive and metabolic functions in birds. This study investigated the morphogenesis and ultrastructural differentiation of the quail pancreas. Fifty-eight quail embryos from day 5 to day 17 of incubation were used for this study. The developing pancreas consistently composed of exocrine and endocrine portions. On day 5, primitive pancreatic tubules (duct-like structures) appeared within the duodenal mesenchyme, lined with pseudostratified and simple columnar epithelium, and associated with early endocrine clusters representing the islets of Langerhans. By day 6, the pancreas was enclosed by a delicate capsule, and the pancreatic cells became cuboidal to pyramidal with prominent basally located nuclei. Progressive lobulation occurred from days 8 and 9, forming dorsal, ventral, third, and splenic lobes with increased vascularization and telocytes distribution. On 10th day of incubation, four distinct lobes were established, and each lobe had its own capsule on 14th day. By day 17, mature serous acini exhibited basophilic basal cytoplasm and apical zymogen granules, indicating functional secretory activity. Ultrastructurally, exocrine acinar cells displayed abundant rough endoplasmic reticulum, prominent nuclei, and diverse zymogen granules. Centroacinar and intercalated ductal cells formed the initial ductal network. Telocytes appeared in perivascular and interacinar regions, suggesting a regulatory role in tissue organization. Endocrine islets were made of α-, β-, and δ-cells, each possessing distinct secretory granules corresponding to glucagon, insulin, and somatostatin secretion, respectively. Grossly, the pancreas developed progressively between the duodenal limbs and attained distinct lobular organization by hatching. These findings elucidate the prehatching structural maturation of the quail pancreas, establishing a foundation for understanding avian pancreatic development and function.

鸟类胰腺的器官发生是确保鸟类正常消化和代谢功能的关键发育过程。研究了鹌鹑胰腺的形态发生和超微结构分化。58只鹌鹑胚胎从孵化第5天至第17天用于本研究。胰腺发育中的胰腺始终由外分泌和内分泌部分组成第5天，十二指肠间质内出现原始胰小管（管状结构），内衬假层状上皮和单柱状上皮，并伴有早期内分泌团，代表朗格汉斯岛。第6天，胰腺被一层精致的被膜包裹，胰腺细胞变为立方体到锥体，细胞核位于基部。从第8天和第9天开始出现渐进式小叶，形成背叶、腹叶、第三叶和脾叶，血管化和远端细胞分布增加。培养第10天，形成4个不同的裂片，第14天每个裂片都有自己的被囊。到第17天，成熟浆液腺泡呈现出嗜碱性的基细胞质和顶端酶原颗粒，显示出功能性分泌活性。在超微结构上，外分泌腺泡细胞显示丰富的粗糙内质网，突出的细胞核和多种酶原颗粒。中央腺泡和嵌入的导管细胞形成了最初的导管网络。远端细胞出现在血管周围和腺泡间区，提示其在组织中起调节作用。内分泌胰岛由α-细胞、β-细胞和δ-细胞组成，它们各自具有不同的分泌颗粒，分别对应于胰高血糖素、胰岛素和生长抑素的分泌。大体而言，胰腺在十二指肠肢之间逐渐发育，并通过孵化形成明显的小叶组织。这些发现阐明了鹌鹑胰腺在孵化前的结构成熟，为了解鸟类胰腺的发育和功能奠定了基础。

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引用次数: 0

Oropharyngeal cavity roof adaptations of the migratory black-tailed native-hen (Tribonyx ventralis): Gross, scanning electron microscopic, and histological features 迁徙的黑尾本地母鸡（Tribonyx ventralis）的口咽腔顶部适应性：大体、扫描电镜和组织学特征

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell

Pub Date : 2026-01-23 DOI: 10.1016/j.tice.2026.103333

Ramadan Kandyel , Saeed Alasmari , Salem H. Alharethi , Abdulrhman A. Almadiy , Bader Albogami , Diaa Massoud , Basma G. Hanafy , Ahmed G. Nomir , Mohamed Abumandour

The morphological structure of the oropharyngeal cavity roof of the Black-tailed Native-Hen (Tribonyx ventralis) had not been previously researched. The study aimed to provide a comprehensive morphological description of the oropharyngeal cavity roofs of the Black-tailed Native-Hen using gross, SEM, and histological techniques. The oropharyngeal roof was categorized into three regions: pre-conchal, middle conchal, and infundibular. The dorsal palatine surface carried twelve papillary rows: eleven longitudinal and one transverse. These rows were presented in a manner such that one median longitudinal row was on the pre-choanal region, eight longitudinal rows were on the choanal field on each side of the choanal opening, two longitudinal rows bordered the rostral portion of the choanal opening, and one transverse row separated the rostral and caudal portions. Papillae are arranged in twelve rows on the palatine surface, with free distribution in specific lateral areas, while the caudal choanal and infundibular regions lack papillae. The choanal opening was located in the caudal palatine half, which was divided into two parts: the rostral long papillary and the caudal wide non-papillary portions. Histologically, the salivary glands were classified into five types based on their position: rostral, median, caudal, intraepithelial, and infundibular. The oropharyngeal roof, influenced by various lifestyles and environments, significantly influences food handling and direction toward the esophagus.

黑尾土母鸡（Tribonyx ventralis）口咽腔顶部的形态结构以前没有研究过。本研究旨在利用大体、扫描电镜和组织学技术对黑尾土鸡的口咽腔顶部进行全面的形态学描述。口咽顶分为三个区域：喉前、喉中、喉下。腭背表面有十二排乳突：十一排纵向，一排横向。这些行以这样的方式呈现：一条中间纵行位于后鼻孔区域，八条纵行位于后鼻孔开口两侧的后鼻孔区域，两条纵行与后鼻孔开口的吻侧部分接壤，一条横行将吻侧和尾侧部分分开。乳突在腭表面呈12排排列，在特定外侧区域自由分布，而尾侧后孔区和漏斗区缺乏乳突。后肛门开口位于腭尾部，分为吻侧长乳头状部分和尾侧宽非乳头状部分两部分。组织学上，唾液腺根据其位置分为五种类型：吻侧、正中、尾侧、上皮内和漏斗。口咽顶部受各种生活方式和环境的影响，显著影响食物的处理和流向食道。

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引用次数: 0

Ultrastructural features of the excurrent ducts of the testis of a wild bird, the cattle egret (Bubulcus ibis) 野鸟牛白鹭（Bubulcus ibis）睾丸排出管的超微结构特征

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell

Pub Date : 2026-01-22 DOI: 10.1016/j.tice.2026.103329

Narindra H. Roopnarine , Tom A. Aire , Antoinette V. Lensink , Sunil K. Gupta , Curtis.E. Hopkins , Matthew B. Charles

The structure and functions of components of the excurrent ducts of the testis of birds are still poorly understood. Most of the few reports on these ducts are on domesticated avian species. This report on the cattle egret is one of the very few studies on wild birds. Tissues from the ducts of five sexually mature and active male birds were routinely prepared and stained for light and transmission electron microscopy. The epididymis and ductus deferens of the cattle egret are generally similar structurally to those reported for domestic birds, with a number of cellular differences. The epithelium of certain segments of the egret’s rete testis displayed numerous, large intercellular spaces, not usually observed in most domestic avian species. As in the domestic species of birds, the proximal efferent ducts in the cattle egret displayed a robust endocytic apparatus as well as abundant lysosomes, but there were very few heterolysosomes and telolysosomes, which were common in domestic species. The border between the proximal and distal efferent ducts also demonstrated active spermiophagy. In addition to evidence of moderate secretary activities, the presence of extremely large heterolysosomes and telolysosomes also demonstrated the endocytic and digestive ability of cells lining the ductus deferens.

鸟类睾丸排出管组成部分的结构和功能尚不清楚。关于这些管道的少数报告大多是关于驯养鸟类的。这份关于牛白鹭的报告是为数不多的关于野生鸟类的研究之一。从五只性成熟和活跃的雄鸟的导管中常规制备组织，并在光镜和透射电子显微镜下染色。牛白鹭的附睾和输精管在结构上与家禽相似，但在细胞上存在一些差异。白鹭睾丸网的某些部分的上皮显示出大量的、大的细胞间隙，这在大多数家禽物种中是不常见的。与家养鸟类一样，牛白鹭的近端传出管显示出强大的内吞装置和丰富的溶酶体，但很少有家养鸟类常见的异溶酶体和端溶酶体。近端和远端传出管之间的边界也表现出活跃的噬精行为。除了中等分泌活性的证据外，超大的异溶酶体和端溶酶体的存在也表明了内衬管细胞的内吞和消化能力。

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引用次数: 0