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The influence of vitamin C and early-age thermal conditioning on the quality of meat and specific production characteristics of broilers during heat stress 维生素C和早期热应激对热应激肉鸡肉品质和特定生产特性的影响
IF 0.6 4区 农林科学 Q3 Veterinary Pub Date : 2020-03-01 DOI: 10.3906/vet-1905-95
Z. Ružić, Z. Kanački, M. Jokanović, S. Vidaković, S. Knežević, S. Jović, S. Paraš
Heat stress HS is one of the greatest problems in contemporary chicken production and it entails significant economic losses. The purpose of this paper is to examine the influence of early-age thermal conditioning ETC , vitamin C Vit C supplementation, and their combination on the production characteristics and specific quality parameters of the meat of broilers which were exposed to chronic HS in the last two weeks of breeding. Four hundred broilers Cobb 500 of both sexes were divided into 4 experimental groups. Group C was given Vit C 2 g/L dissolved in water from day 22 until the end of production. Group T was exposed to ETC for a period of 24 h at the temperature of 38 ± 1 oC and 40%-60% relative humidity on the fifth day of breeding. Group TC was the combination of the groups T and C, while group K was the control group. The results indicate that ETC, independently or in combination with Vit C, improves the production characteristics in terms of feed conversion reduction P < 0.05 . The examined treatments increase the volume P < 0.05 of certain parts of the body carcass, legs and thighs, and back with regards to total body weight, especially in group TC. Regarding meat quality determined according to pH value and meat color criteria CIEL* , the best results were also observed in group TC, followed by groups T and C. These results justify the use of Vit C and ETC in summer conditions, when heat stress is expected; however, the best results can be achieved by combining these two methods, thus producing a synergistic effect.
热应激是当代鸡肉生产中最大的问题之一,它会造成重大的经济损失。本试验旨在研究早期热调节、维生素C、维生素C补充及其组合对饲养后期暴露于慢性HS的肉鸡的生产特性和特定品质参数的影响。选取400只雄性科布500肉鸡,分为4个试验组。C组从第22天开始给予水溶维生素C 2 g/L,直至生产结束。T组于繁殖第5天,在温度为38±1℃,相对湿度为40% ~ 60%的条件下,ETC暴露24 h。TC组为T组和C组的结合,K组为对照组。结果表明,在降低饲料系数方面,ETC单独或与Vit C联合使用均能改善生产特性,P < 0.05。各处理对胴体某些部位、腿和大腿、背部的体积和总重的影响均有显著提高(P < 0.05),以TC组尤显著。根据pH值和肉色标准CIEL*测定的肉质,TC组效果最好,其次是T组和C组。这些结果证明在夏季条件下使用Vit C和ETC是合理的,因为夏季条件下会出现热应激;然而,将这两种方法结合起来可以达到最佳效果,从而产生协同效应。
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引用次数: 3
First molecular characterization of the facultative myiasis agent Eristalis tenax in Turkey using mitochondrial cytochrome c oxidase subunit I DNA barcoding sequences 利用线粒体细胞色素c氧化酶亚基I DNA条形码序列首次对土耳其兼性丝虫病剂Eristalis tenax进行分子表征
IF 0.6 4区 农林科学 Q3 Veterinary Pub Date : 2020-03-01 DOI: 10.3906/vet-1911-82
Mübeccel Okur, A. Yıldırım, A. Inci
This report is the first molecular characterization of the facultative myiasis agent Eristalis tenax collected from cattle barns in Kayseri province of Central Anatolia, Turkey. A total of 10 adult fly specimens were included in the study. All flies were identified as female adults of E. tenax by morphologic characteristics under a stereomicroscope. The barcode region of the mitochondrial cytochrome oxidase 1 CO1 gene was amplified with PCR from the genomic DNA extracts of E. tenax specimens for molecular identification and phylogenetic analyses. There was no polymorphism among the CO1 sequences of all isolates leading to the detection of a single haplotype for E. tenax. Haplotype and nucleotide diversities in the CO1 data set comprised of the sequence of the detected haplotype and the published sequences of E. tenax from different countries in GenBank were determined as 0.775 and 0.002, respectively. The newly characterized haplotype in this study clustered with the haplotypes from Australia and Canada in a monophyletic clade and exhibited a 100.0% identity to the Australian E. tenax isolate JN991985 . Interspecific genetic differences between E. tenax and other Eristalis species were in the range of 5.7% to 7.9%.
本报告是首次对从土耳其安纳托利亚中部开塞利省牛舍中采集的兼性蝇蛆病剂Eristalis tenax进行分子鉴定。本研究共收集成蝇标本10份。在体视显微镜下通过形态学特征鉴定所有蝇类均为雌性成虫。用PCR方法从田鼠标本的基因组DNA提取物中扩增出线粒体细胞色素氧化酶1 CO1基因的条形码区,并进行分子鉴定和系统发育分析。所有分离株的CO1序列均不存在多态性,均为单倍型。在由检测到的单倍型序列和GenBank中来自不同国家的E. tenax序列组成的CO1数据集中,单倍型和核苷酸多样性分别为0.775和0.002。新发现的单倍型与来自澳大利亚和加拿大的单倍型聚集在一个单系分支中,与澳大利亚的E. tenax分离物JN991985的同源性为100.0%。种间遗传差异在5.7% ~ 7.9%之间。
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引用次数: 0
DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance DMEM和FBS作为猪冷冻精液解冻液,可以提高精子活力和母猪繁殖性能
IF 0.6 4区 农林科学 Q3 Veterinary Pub Date : 2020-03-01 DOI: 10.3906/vet-1910-100
Peng Zheng, Ziming Wang, Xiaoyu Li, Fushuo Huang, Mingjun Ma, He Huang
The purpose of this experiment was to investigate the effects of Beltsville thawing solution BTS , Androhep, and Dulbecco?s modified Eagle medium + Fatal bovine serum D-F as diluents of frozen semen on sperm motility and sow reproductive performance. In experiment 1, boar semen was collected and diluted to 2.0 × 109/60 mL, 1.5 × 109/60 mL, and 1.5 × 109/40 mL with Androhep. The appropriate semen dose was determined by postcervical insemination. In experiment 2, boar semen was fumigated with liquid nitrogen and thawed semen was diluted to 1.5 × 109/40 mL with D-F, Androhep, and BTS, respectively. The effects of above diluents on sperm motility and sow reproductive performance were studied by postcervical insemination. The appropriate semen dose for postcervical insemination was 1.5 × 109/40 mL. After thawing of frozen semen, sperm motility of semen diluted with D-F was significantly higher than that of semen diluted with Androhep and BTS. There was no significant difference in pregnancy rates among sows via artificial insemination. The litter size of sows using D-F diluted semen was significantly higher than that of sows using Androhep and BTS diluted semen. In conclusion, D-F freezing diluent can improve sperm motility of frozen boar semen after thawing, prolong sperm survival time, and increase sow reproductive performance.
本实验旨在探讨Beltsville解冻液BTS、Androhep和Dulbecco?s改良Eagle培养基+致命牛血清D-F作为冷冻精液稀释剂对精子活力和母猪繁殖性能的影响。试验1收集公猪精液,用Androhep稀释至2.0 × 109/ 60ml、1.5 × 109/ 60ml和1.5 × 109/ 40ml。通过子宫颈后人工授精确定合适的精液剂量。试验2用液氮熏蒸公猪精液,解冻后的精液分别用D-F、Androhep和BTS稀释至1.5 × 109/40 mL。通过子宫颈后人工授精研究了上述稀释剂对母猪精子活力和繁殖性能的影响。经冷冻精子解冻后,经D-F稀释后的精子活力显著高于经Androhep和BTS稀释后的精子活力。人工授精母猪的受孕率无显著差异。使用D-F稀释精液的母猪产仔数显著高于使用Androhep和BTS稀释精液的母猪。综上所述,D-F冷冻稀释液能提高冷冻猪精液解冻后的精子活力,延长精子存活时间,提高母猪繁殖性能。
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引用次数: 0
Growth performance, survival rate, flesh, and proximate composition of sex-grouped triploid and diploid Nile tilapia (Oreochromis niloticus) 尼罗罗非鱼三倍体和二倍体的生长性能、存活率、肉质和近似组成
IF 0.6 4区 农林科学 Q3 Veterinary Pub Date : 2020-03-01 DOI: 10.3906/vet-1905-79
A. Mukti, O. Carman, A. Alimuddin, M. Zairin, M. Suprayudi
This study aimed to compare the growth performance, survival rate, flesh, and proximate composition of sex-grouped triploid and diploid Nile tilapia. The triploid population was obtained through heat shock at 41 oC for 4 min, 4 min after fertilization. Before sexing, 50 fish were reared in aquaria at a density of 1 fish L$^{-1}$ for 2 months. After sexing, both triploid and diploid fish were grouped into all-male, all-female, and mixed-sex groups and reared in hapas at a density of 10 fish m$^{-2}$ for 4 months. Each group was replicated three times. The highest body weight, body length, and growth rate were observed in all-male triploids, while the lowest of those parameters were obtained in all-female diploids. The highest survival rate was achieved in both all-male and mixed-sex triploids, and it did not significantly differ from the mixed-sex diploid P > 0.05 . The triploid fish had a higher edible carcass percentage than diploids. Proximate analysis indicated that the crude protein content of triploids was higher than that of diploids, while the crude lipid and ash contents were lower than those of diploids P < 0.05 . Triploid Nile tilapia had the best growth performances, including flesh quantity and quality, compared to diploids.
本研究旨在比较三倍体和二倍体尼罗罗非鱼的生长性能、存活率、肉质和近似组成。受精后4 min,在41℃下热休克4 min,获得三倍体群体。性别鉴定前,50尾鱼在水族箱中以1尾鱼的密度L$^{-1}$饲养2个月。雌雄鉴定后,将三倍体和二倍体鱼分成全雄、全雌和混合性别组,以10尾m$^{-2}$的密度饲养4个月。每组重复3次。全雄性三倍体的体重、体长和生长率最高,全雌性二倍体的体重、体长和生长率最低。全雄性和混合性三倍体的成活率最高,与混合性二倍体差异不显著P > 0.05。三倍体鱼的可食用胴体率高于二倍体鱼。近似分析表明,三倍体的粗蛋白质含量高于二倍体,粗脂肪和粗灰分含量低于二倍体,P < 0.05。与二倍体相比,三倍体尼罗罗非鱼的生长性能最好,包括肉的数量和品质。
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引用次数: 3
Cranial size and shape sexual dimorphism in the Kangal dog from Turkey 土耳其坎加尔犬头颅大小和形状的两性二态性
IF 0.6 4区 农林科学 Q3 Veterinary Pub Date : 2020-03-01 DOI: 10.3906/vet-1907-86
P. Parés-Casanova, A. Siddiq, V. Onar
This study has so far been the first attempt to characterize and quantify skull sexual variation in Kangal dog, by means of geometric morphometric techniques. A sample of 16 adult Kangal crania has been analyzed with this purpose. To obtain a full image of morphological pattern, digital pictures were taken from the ventral, left lateral, and dorsal sides of each skull, and a total of 16, 15, and 16 landmarks respectively were obtained on each image. Skull size and shape differed significantly in all aspects among different sexes, male skulls being bigger. Shape differences were observed mainly on zygomatic arch and muzzle on the dorsal view, pterygoid bone and articular surface to mandibular condyle in the lateral aspect, and cranial width and maxillary bone on the ventral view. Although the sample was comparatively small in number, being the first geometric morphometric approach applied on the Kangal dog, the obtained results will add vital information particularly to understand the cranial shape sexual dimorphism of this unique dog breed in Turkey.
到目前为止,这项研究是第一次尝试用几何形态测量技术来表征和量化坎加尔狗的头骨性别差异。为此目的,对16个成年袋鼠颅骨样本进行了分析。为了获得形态学模式的完整图像,我们从每个颅骨的腹侧、左外侧和背侧分别拍摄了数字图像,每张图像上分别获得了16、15和16个地标。不同性别间颅骨大小和形状各方面差异显著,男性颅骨较大。在背侧观上主要观察到颧弓和口鼻的形状差异,在侧面观察到翼状骨和关节面与下颌髁的形状差异,在腹侧观上主要观察到颅宽和上颌骨的形状差异。虽然样本数量相对较少,但作为第一个应用于坎加尔犬的几何形态计量学方法,所获得的结果将增加重要信息,特别是了解土耳其这种独特犬种的颅骨形状性别二态性。
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引用次数: 6
Determination of some coagulation parameters according to age and sex in Sivas Kangal dogs 西瓦斯康加尔犬按年龄和性别的凝血参数测定
IF 0.6 4区 农林科学 Q3 Veterinary Pub Date : 2020-03-01 DOI: 10.3906/vet-1811-29
Burcu Insal, I. Pi̇şki̇n
Hemostasis is stopping bleeding in a controlled manner whereas coagulation is a series criterion and to diagnose disorders caused by coagulation in an early period is to determine the coagulation time. These phases are controlled with various tests. In this study, the aim is to determine some coagulation parameters in Sivas Kangal dogs by taking the age and sex into consideration. Coagulation is vitally important in many physiological and pathological cases. The most important tests, complete blood count with peripheral blood smear, prothrombin time PT , activated partial thromboplastin time aPTT , and D-dimer fibrin degradation product assay were applied to all subjects, respectively. It was found that thrombocyte counts in Sivas Kangal dogs were affected by the age factor in automatic and manual counting, whereas they were not affected by sex. In the same way, while PT times showed a meaningful change among groups depending on age, aPTT times were not affected by sex or age. The D-dimer assay results, which show the existence of fibrin degradation product, change depending on age and sex.
止血是有控制地止血,凝血是一系列的标准,早期诊断凝血引起的疾病就是确定凝血时间。这些阶段由各种测试控制。在本研究中,目的是在考虑年龄和性别的情况下确定Sivas Kangal犬的一些凝血参数。凝血在许多生理和病理病例中都是至关重要的。最重要的测试,外周血涂片全血细胞计数,凝血酶原时间PT,活化部分凝血活酶时间aPTT和d -二聚体纤维蛋白降解产物测定分别应用于所有受试者。结果发现,在自动和手动计数中,Sivas Kangal犬的血小板计数受年龄因素的影响,而不受性别的影响。同样地,虽然不同年龄的组间PT时间有显著的变化,但aPTT时间不受性别或年龄的影响。d -二聚体测定结果显示纤维蛋白降解产物的存在,随年龄和性别而变化。
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引用次数: 1
Arsenic, cadmium, mercury, and lead in date mussels from the Sarajevo fish market (Bosnia and Herzegovina): a preliminary study on the health risks 萨拉热窝鱼市(波斯尼亚和黑塞哥维那)枣贻贝中砷、镉、汞和铅:健康风险的初步研究
IF 0.6 4区 农林科学 Q3 Veterinary Pub Date : 2020-03-01 DOI: 10.3906/vet-1908-13
E. Omeragić, A. Marjanović, Jasmina Djedjibegovic, A. Turalić, A. Čaušević, H. Niksic, F. Čaklovica, M. Šober
The main objective of this study was to determine the content of heavy metals Cd, Pb, Hg and metalloid As in samples of date mussels Lithophaga lithophaga L., 1758 in order to evaluate the health risks for mussel consumers. Samples of shellfish were obtained from commercial sources and fishery in the area of Sarajevo. The content of heavy metals and arsenic is determined in a total of 46 samples. Samples were prepared by microwave digestion. For the analysis of total As, cadmium and lead, an atomic absorption spectrophotometer with graphite furnace was used, while mercury content was determined using a direct Hg analyser. In all samples, the content of heavy metals and arsenic was above the quantification limit of the given methods. The content of all analysed metals and metalloid was below the limits of their maximum allowable concentration in food, according to the current regulations in Bosnia and Herzegovina and the European Union. Evaluation of the public health risks associated with date mussels' consumption indicates that there is no evident risk for a moderate adult consumer.
本研究的主要目的是测定1758年份贻贝样品中重金属Cd、Pb、Hg和类金属As的含量,以评价贻贝消费者的健康风险。贝类样本是从萨拉热窝地区的商业来源和渔业获得的。共对46个样本进行重金属和砷含量测定。采用微波消解法制备样品。总砷、镉和铅的分析采用石墨炉原子吸收分光光度计,汞的测定采用直接汞分析仪。所有样品中重金属和砷的含量均超过了给定方法的定量限。根据波斯尼亚-黑塞哥维那和欧洲联盟的现行条例,所有经分析的金属和类金属的含量都低于食品中允许的最高浓度。对与食用枣贻贝相关的公共健康风险的评估表明,对中等成年消费者没有明显的风险。
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引用次数: 1
Evaluation of gait character of Akbaş and Kangal shepherd dogs by using pressure-sensitive walkway 用压敏步道评价akbaku和Kangal牧羊犬的步态特征
IF 0.6 4区 农林科学 Q3 Veterinary Pub Date : 2020-03-01 DOI: 10.3906/vet-1911-56
Ozan Gündemir, H. Alpak, D. O. Erdikmen, Didar Aydin Kaya
In this study, it was aimed to evaluate which walking analysis data may be obtained for Kangal and Akbas shepherd dogs using a pressure-sensitive walkway PSW and which of these data may be used in the fields of veterinary anatomy, orthopedics, and neurology. Center of pressure COP analysis results of 46 dogs were examined. Distance type gait parameters of dogs were measured and their foot zone analysis values were recorded. It was observed that the pressure values applied by forelimb steps on the ground were higher compared to the pressure applied by hindlimb steps. The highest peak vertical force PVF values were observed in DP3 and DP4 for both forelimbs and hindlimbs. It was determined that the pressure value applied by metapodial pads on the ground were lower compared to digital pads and the PVF values of metapodial pads of the hindlimb were higher than the forelimb metapodial pad values. The correlation between weight and forelimb pressure values was higher than the correlation values between weight and hindlimb pressure values. With the pressure-sensitive walkway system used in the study, COP and foot zone analysis may be performed and distance gait parameters may be measured. It is considered that this system, in which more data may be obtained about the morphometry of walking, is more advantageous with its ease of use compared to other walking analysis systems. It is considered that the studies performed with this system would be a reference in veterinary orthopedic and neurology fields and would contribute to the development of the usage of pressure-sensitive walkway systems for dogs.
在本研究中,目的是评估使用压力敏感行走通道PSW可以获得Kangal和Akbas牧羊犬的哪些行走分析数据,以及哪些数据可以用于兽医解剖学、骨科和神经病学领域。对46只犬进行压力中心COP分析。测量犬的距离型步态参数,记录其足区分析值。观察到,前肢台阶对地面施加的压力值高于后肢台阶施加的压力值。前肢和后肢DP3和DP4均观察到最高垂直力PVF值。结果表明,后肢跖骨垫对地面施加的压力值低于数字垫,后肢跖骨垫的PVF值高于前肢跖骨垫。体重与前肢压力值的相关性高于体重与后肢压力值的相关性。通过研究中使用的压力敏感步行系统,可以进行COP和足区分析,并可以测量距离步态参数。该系统可以获得更多关于步行形态的数据,与其他步行分析系统相比,其易用性更具优势。该系统的研究将为兽医骨科和神经学领域提供参考,并有助于犬用压力敏感通道系统的发展。
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引用次数: 0
Study of mitochondrial DNA (mtDNA) D - loop region polymorphism in Şavak Akkaraman sheep Şavak阿卡拉曼羊线粒体DNA (mtDNA) D环区多态性研究
IF 0.6 4区 农林科学 Q3 Veterinary Pub Date : 2020-03-01 DOI: 10.3906/vet-1905-57
S. Yagci, S. Baş, Selahaddin Ki̇raz
This study was conducted to investigate the maternal genetic diversity using the mtDNA D - loop region 842 base pair bp polymorphism in Şavak Akkaraman sheep raised in the province of Erzincan. According to the results, in the mtDNA D - loop of the Şavak Akkaraman breed, 27 polymorphic sites and 20 haplotypes were identified and then, 11 of the 20 haplotypes identified in Şavak sheep were in haplogroup B 55% , 6 in haplogroup A 30% , and 3 in haplogroup C 15% . Haplogroup B was the most frequent haplogroup in Şavak Akkaraman sheep. Haplotype and nucleotide diversities were estimated to be 0.995 ± 0.004 and 0.0146 ± 0.0003, respectively. The Şavak Akkaraman breed was compared with the mtDNA sequencing of different breeds and wild sheep, and the contribution of the breed to biodiversity was considered.
利用Erzincan省Şavak阿卡拉曼羊的mtDNA D -环区842碱基对bp多态性,对母系遗传多样性进行了研究。结果表明,在Şavak Akkaraman羊的mtDNA D -环中,鉴定出27个多态性位点和20个单倍型,其中,Şavak羊鉴定出的20个单倍型中,有11个在单倍群B中占55%,6个在单倍群A中占30%,3个在单倍群C中占15%。单倍群B是Şavak阿卡拉曼羊中最常见的单倍群。单倍型和核苷酸多样性分别为0.995±0.004和0.0146±0.0003。将Şavak Akkaraman品种与不同品种和野生羊的mtDNA序列进行比较,考虑该品种对生物多样性的贡献。
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引用次数: 2
Mobile applications to obtain minimum cost feed mixes 移动应用程序获得最低成本的饲料混合
IF 0.6 4区 农林科学 Q3 Veterinary Pub Date : 2020-03-01 DOI: 10.3906/vet-1912-31
M. Boğa, K. K. Çevik, Hasan Önder
In this study, ration preparation software to minimize the cost of feed for ruminant livestock such as cattle, sheep, and goats for both milk and meat yield was developed for Web- and Android-based systems using genetic algorithms. To maximize accessibility on PCs, smartphones, and tablet PCs, we used Web- and Android-based software to find cheaper feed mixes that satisfy the nutritional requirements of ruminants. With this novel system, farmers and scientists can obtain low-cost feed mixes via the Web or smartphones, regardless of time or location. This application is useful for feed producers and farmers because they can use this software from any location and at any time. Users can input their new feed resources for preparing rations
在本研究中,利用遗传算法为基于Web和android的系统开发了口粮制备软件,以最大限度地降低牛、绵羊和山羊等反刍动物的饲料成本,从而提高奶和肉的产量。为了最大限度地提高个人电脑、智能手机和平板电脑的可访问性,我们使用基于Web和android的软件来寻找满足反刍动物营养需求的更便宜的饲料混合物。有了这个新系统,农民和科学家可以通过网络或智能手机获得低成本的饲料混合物,而不受时间和地点的限制。这个应用程序对饲料生产商和农民很有用,因为他们可以在任何地点和任何时间使用这个软件。用户可以输入新的饲料资源来准备口粮
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引用次数: 1
期刊
Turkish Journal of Veterinary & Animal Sciences
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