Pub Date : 2021-07-01DOI: 10.1158/1538-7445.AM2021-3147
F. Barthel, N. Verburg, R. Rockne, R. Eijgelaar, K. Anderson, D. J. Müller, S. Branciamore, K. Johnson, P. Wesseling, P. D. W. Hamer, R. Verhaak
{"title":"Abstract 3147: Stereotactic image-guided epigenome profiling reveals a neural stem cell evolutionary origin of diffuse gliomas","authors":"F. Barthel, N. Verburg, R. Rockne, R. Eijgelaar, K. Anderson, D. J. Müller, S. Branciamore, K. Johnson, P. Wesseling, P. D. W. Hamer, R. Verhaak","doi":"10.1158/1538-7445.AM2021-3147","DOIUrl":"https://doi.org/10.1158/1538-7445.AM2021-3147","url":null,"abstract":"","PeriodicalId":23364,"journal":{"name":"Tumor Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42241280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-01DOI: 10.1158/1538-7445.AM2021-2891
N. Li, Shile Huang
Metastasis has been considered as one of the cancer hallmarks. Approximately 90% of cancer-related death is due to cancer metastasis. Tumor cell migration is a prerequisite for cancer metastasis. Protein phosphatase 5 (PP5) is a serine/threonine protein phosphatase that belongs to the phosphoprotein phosphatase (PPP) family including PP1 and PP2A. It is well known that PP5 regulates apoptosis, proliferation, and stress response. However, the role of PP5 in the regulation of cell motility is only beginning to be recognized, and the mechanism of PP5 regulation of cell motility is largely unknown. In this study, our tissue microarray (TMA) studies showed the expression of PP5 increased with the stages of prostate cancer, which is consistent with the previous findings that there is a positive correlation between PP5 overexpression and breast cancer metastases. Furthermore, by the single-cell motility assay, we found that overexpression of PP5 increased the motility of various tumor cells, whereas knockdown of PP5 decreased the cell motility, suggesting that PP5 positively regulates cell motility. As focal adhesion kinase (FAK) is a key regulator of cell motility, and a report has shown that PP5 forms a complex with ASK1, FAK, CXCR4 in wounded alveolar epithelial cancer cells, we examined whether PP5 activates FAK. The Western blotting results showed that overexpression of PP5 did not obviously affect the phosphorylation of FAK. The results suggest that PP5 regulates cell motility through a new mechanism. Further research is on the way to identify how PP5 positively regulates cell motility. (Supported by the Feist-Weiller Cancer Center, LSU Health Sciences Center, Shreveport, LA, USA.) Citation Format: n Li, Shile Huang. Protein phosphatase 5 regulation of cell motility [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2021; 2021 Apr 10-15 and May 17-21. Philadelphia (PA): AACR; Cancer Res 2021;81(13_Suppl):Abstract nr 2891.
{"title":"Abstract 2891: Protein phosphatase 5 regulation of cell motility","authors":"N. Li, Shile Huang","doi":"10.1158/1538-7445.AM2021-2891","DOIUrl":"https://doi.org/10.1158/1538-7445.AM2021-2891","url":null,"abstract":"Metastasis has been considered as one of the cancer hallmarks. Approximately 90% of cancer-related death is due to cancer metastasis. Tumor cell migration is a prerequisite for cancer metastasis. Protein phosphatase 5 (PP5) is a serine/threonine protein phosphatase that belongs to the phosphoprotein phosphatase (PPP) family including PP1 and PP2A. It is well known that PP5 regulates apoptosis, proliferation, and stress response. However, the role of PP5 in the regulation of cell motility is only beginning to be recognized, and the mechanism of PP5 regulation of cell motility is largely unknown. In this study, our tissue microarray (TMA) studies showed the expression of PP5 increased with the stages of prostate cancer, which is consistent with the previous findings that there is a positive correlation between PP5 overexpression and breast cancer metastases. Furthermore, by the single-cell motility assay, we found that overexpression of PP5 increased the motility of various tumor cells, whereas knockdown of PP5 decreased the cell motility, suggesting that PP5 positively regulates cell motility. As focal adhesion kinase (FAK) is a key regulator of cell motility, and a report has shown that PP5 forms a complex with ASK1, FAK, CXCR4 in wounded alveolar epithelial cancer cells, we examined whether PP5 activates FAK. The Western blotting results showed that overexpression of PP5 did not obviously affect the phosphorylation of FAK. The results suggest that PP5 regulates cell motility through a new mechanism. Further research is on the way to identify how PP5 positively regulates cell motility. (Supported by the Feist-Weiller Cancer Center, LSU Health Sciences Center, Shreveport, LA, USA.) Citation Format: n Li, Shile Huang. Protein phosphatase 5 regulation of cell motility [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2021; 2021 Apr 10-15 and May 17-21. Philadelphia (PA): AACR; Cancer Res 2021;81(13_Suppl):Abstract nr 2891.","PeriodicalId":23364,"journal":{"name":"Tumor Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42267807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-01DOI: 10.1158/1538-7445.AM2021-2709
Fang Liu, Jun Zhou, Tanima Kundu-Roy, Joseph Wahler, J. So, Yong Lin, Y. Lou, N. Barnard, I. Matsuura, N. Suh
{"title":"Abstract 2709: CD44 inhibits DCIS progression to invasive carcinoma","authors":"Fang Liu, Jun Zhou, Tanima Kundu-Roy, Joseph Wahler, J. So, Yong Lin, Y. Lou, N. Barnard, I. Matsuura, N. Suh","doi":"10.1158/1538-7445.AM2021-2709","DOIUrl":"https://doi.org/10.1158/1538-7445.AM2021-2709","url":null,"abstract":"","PeriodicalId":23364,"journal":{"name":"Tumor Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49570385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-01DOI: 10.1158/1538-7445.AM2021-2727
Vasiliki Stravokefalou, D. Stellas, S. Karaliota, Bethany A. Nagy, T. Guerin, S. Kozlov, B. Felber, G. Pavlakis
{"title":"Abstract 2727: Heterodimeric IL-15 (hetIL-15) affects conventional dendritic cells and a distinct novel dendritic cell population in different mouse cancer models of breast and pancreatic cancer","authors":"Vasiliki Stravokefalou, D. Stellas, S. Karaliota, Bethany A. Nagy, T. Guerin, S. Kozlov, B. Felber, G. Pavlakis","doi":"10.1158/1538-7445.AM2021-2727","DOIUrl":"https://doi.org/10.1158/1538-7445.AM2021-2727","url":null,"abstract":"","PeriodicalId":23364,"journal":{"name":"Tumor Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49611457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-01DOI: 10.1158/1538-7445.AM2021-130
Ansam Sinjab, Guangchun Han, Warapen Treekitkarnmongkol, K. Hara, P. Brennan, M. Dang, Dapeng Hao, Ruiping Wang, E. Dai, H. Dejima, Jiexin Zhang, Elena P. Bogatenkova, B. Sánchez-Espiridión, K. Chang, Danielle R. Little, Samer Bazzi, L. Tran, K. Krysan, C. Behrens, D. Duose, E. Parra, M. G. Raso, L. Solis, J. Fukuoka, Jianjun Zhang, B. Sepesi, T. Cascone, L. Byers, D. Gibbons, Jichao Chen, S. Moghaddam, E. Ostrin, D. Rosen, J. Heymach, P. Scheet, S. Dubinett, I. Wistuba, J. Fujimoto, Christopher S Stevenson, A. Spira, Linghua Wang, H. Kadara
{"title":"Abstract 130: Resolving the spatial and cellular architecture of lung adenocarcinoma by multi-region single-cell sequencing","authors":"Ansam Sinjab, Guangchun Han, Warapen Treekitkarnmongkol, K. Hara, P. Brennan, M. Dang, Dapeng Hao, Ruiping Wang, E. Dai, H. Dejima, Jiexin Zhang, Elena P. Bogatenkova, B. Sánchez-Espiridión, K. Chang, Danielle R. Little, Samer Bazzi, L. Tran, K. Krysan, C. Behrens, D. Duose, E. Parra, M. G. Raso, L. Solis, J. Fukuoka, Jianjun Zhang, B. Sepesi, T. Cascone, L. Byers, D. Gibbons, Jichao Chen, S. Moghaddam, E. Ostrin, D. Rosen, J. Heymach, P. Scheet, S. Dubinett, I. Wistuba, J. Fujimoto, Christopher S Stevenson, A. Spira, Linghua Wang, H. Kadara","doi":"10.1158/1538-7445.AM2021-130","DOIUrl":"https://doi.org/10.1158/1538-7445.AM2021-130","url":null,"abstract":"","PeriodicalId":23364,"journal":{"name":"Tumor Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42330859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-01DOI: 10.1158/1538-7445.AM2021-2837
M. Sosa, Carolina Rodriguez-Tirado, Nupura Kale, M. Carlini, N. Shrivastava, Bassem D. Khalil, J. Bravo-Cordero, Melissa Alexander, Jiayi Ji
Cancer cells disseminate during very early and sometimes asymptomatic stages of tumor progression. Granted that biological barriers to tumorigenesis exist, there must also be limiting steps to early dissemination, all of which remain largely unknown. We report that the orphan nuclear receptor NR2F1/COUP-TF1 serves as a barrier to early dissemination. High-resolution intravital imaging revealed that loss of function of NR2F1 in HER2+ early cancer cells increased in vivo dissemination without accelerating mammary tumor formation. NR2F1 expression was positively regulated by the tumor suppressive MMK3/6-p38-MAPK pathway and downregulated by HER2 and Wnt4 oncogenic signaling. NR2F1 downregulation by HER2 in early cancer cells led to decreased E-cadherin expression and β-catenin membrane localization, disorganized laminin 5 deposition, and increased expression of CK14, TWIST1, ZEB1 and PRRX1. Our findings reveal the existence of an inhibitory mechanism of dissemination regulated by NR2F1 downstream of HER2 signaling. Citation Format: Maria Soledad Sosa, Carolina Rodriguez-Tirado, Nupura Kale, Maria Carlini, Nitisha Shrivastava, Bassem Khalil, Javier Bravo-Cordero, Melissa Alexander, Jiayi Ji. NR2F1 is a barrier to early dissemination of pre-malignant mammary cells [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2021; 2021 Apr 10-15 and May 17-21. Philadelphia (PA): AACR; Cancer Res 2021;81(13_Suppl):Abstract nr 2837.
{"title":"Abstract 2837: NR2F1 is a barrier to early dissemination of pre-malignant mammary cells","authors":"M. Sosa, Carolina Rodriguez-Tirado, Nupura Kale, M. Carlini, N. Shrivastava, Bassem D. Khalil, J. Bravo-Cordero, Melissa Alexander, Jiayi Ji","doi":"10.1158/1538-7445.AM2021-2837","DOIUrl":"https://doi.org/10.1158/1538-7445.AM2021-2837","url":null,"abstract":"Cancer cells disseminate during very early and sometimes asymptomatic stages of tumor progression. Granted that biological barriers to tumorigenesis exist, there must also be limiting steps to early dissemination, all of which remain largely unknown. We report that the orphan nuclear receptor NR2F1/COUP-TF1 serves as a barrier to early dissemination. High-resolution intravital imaging revealed that loss of function of NR2F1 in HER2+ early cancer cells increased in vivo dissemination without accelerating mammary tumor formation. NR2F1 expression was positively regulated by the tumor suppressive MMK3/6-p38-MAPK pathway and downregulated by HER2 and Wnt4 oncogenic signaling. NR2F1 downregulation by HER2 in early cancer cells led to decreased E-cadherin expression and β-catenin membrane localization, disorganized laminin 5 deposition, and increased expression of CK14, TWIST1, ZEB1 and PRRX1. Our findings reveal the existence of an inhibitory mechanism of dissemination regulated by NR2F1 downstream of HER2 signaling. Citation Format: Maria Soledad Sosa, Carolina Rodriguez-Tirado, Nupura Kale, Maria Carlini, Nitisha Shrivastava, Bassem Khalil, Javier Bravo-Cordero, Melissa Alexander, Jiayi Ji. NR2F1 is a barrier to early dissemination of pre-malignant mammary cells [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2021; 2021 Apr 10-15 and May 17-21. Philadelphia (PA): AACR; Cancer Res 2021;81(13_Suppl):Abstract nr 2837.","PeriodicalId":23364,"journal":{"name":"Tumor Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42565653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-01DOI: 10.1158/1538-7445.AM2021-2918
Meredith S. Carson, Michael F. Colman, Daniel Roth, Jody E. Albright, Melissa A. VerHague, J. French, S. Hursting
{"title":"Abstract 2918: Characterization of a novel transplantable C3TAg breast cancer model in C57BL/6J mice","authors":"Meredith S. Carson, Michael F. Colman, Daniel Roth, Jody E. Albright, Melissa A. VerHague, J. French, S. Hursting","doi":"10.1158/1538-7445.AM2021-2918","DOIUrl":"https://doi.org/10.1158/1538-7445.AM2021-2918","url":null,"abstract":"","PeriodicalId":23364,"journal":{"name":"Tumor Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42658739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-01DOI: 10.1158/1538-7445.AM2021-2658
A. Schäffer, D. Dominguez, E. Gertz, Lesley M. Chapman, A. Budhu, M. Forgues, Jittiporn Chaisaingmongkol, S. Rabibhadana, Benjarath Pupacdi, Xiaolin Wu, C. Harris, M. Ruchirawat, E. Ruppin, X. Wang
{"title":"Abstract 2658: Oncogenicity of adeno-associated virus (AAV) integration into the genomes of liver cancer patients depends on the overlap between the AAV integration site and the AAV X gene","authors":"A. Schäffer, D. Dominguez, E. Gertz, Lesley M. Chapman, A. Budhu, M. Forgues, Jittiporn Chaisaingmongkol, S. Rabibhadana, Benjarath Pupacdi, Xiaolin Wu, C. Harris, M. Ruchirawat, E. Ruppin, X. Wang","doi":"10.1158/1538-7445.AM2021-2658","DOIUrl":"https://doi.org/10.1158/1538-7445.AM2021-2658","url":null,"abstract":"","PeriodicalId":23364,"journal":{"name":"Tumor Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42728087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-01DOI: 10.1158/1538-7445.AM2021-2661
Yo-taro Shirai, Yoshinori Takizawa, M. Iwadate, Jorge Paiz, Shigetoshi Yokoyama, Masaaki Miyakoshi, J. Ward, S. Kimura
{"title":"Abstract 2661: Role of NKX2-1 in thyroid neoplasms induced by iodine-deficiency and radiation","authors":"Yo-taro Shirai, Yoshinori Takizawa, M. Iwadate, Jorge Paiz, Shigetoshi Yokoyama, Masaaki Miyakoshi, J. Ward, S. Kimura","doi":"10.1158/1538-7445.AM2021-2661","DOIUrl":"https://doi.org/10.1158/1538-7445.AM2021-2661","url":null,"abstract":"","PeriodicalId":23364,"journal":{"name":"Tumor Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47882084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：