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Influence of growth regulators on the seed productivity of medicinal crops grown in the Krasnodar region 生长调节剂对克拉斯诺达尔地区药用作物种子产量的影响
Pub Date : 2023-12-04 DOI: 10.18619/2072-9146-2023-6-71-77
N. Tropina, R. R. Thaganov, V. R. Thaganov, N. I. Sidelnikov
Medicinal plants and preparations thereof are widely used in the treatment and prevention of many diseases. The demand of the pharmaceutical industry for herbal medicinal raw materials is met by culturing these plants. Pharmaceutical factories can be fully supplied with medicinal raw materials both by expanding the area and by increasing the yield of cultivated crops, which is achieved by using high-quality seeds of promising varieties in the required amount. In recent years, in the conditions of the Western Ciscaucasia in the medicinal crop rotation of the North Caucasus branch of VILAR, studies have been conducted to study the possibility of increasing the seed productivity of Echinacea purpurea L., Сhamomilla recutita L., Plantago major L. and Salvia officinalis L., which occupy an important place in the modern range of medicines used in official and traditional medicine. To this end, experiments were laid on the complex test of humic fertilizers (Lignogumat, Normat L) with growth regulators (Zircon, Agat, Gibbersib) and chelated micro-fertilizers (Siliplant and Cytovit)As a result of the studies carried out on echinacea, an increase in the yield of seeds and an improvement in their quality was established on the Lignogumat + Agate variant (by 31%), and on sage and chamomile – Lignogumat + Zircon (by 28% and 39%, respectively), the weight of 1000 seeds exceeded the control by 8-15%. Non-root feeding of echinacea and plantain with Lignogumat and Normat L with silicon-containing microfertilization Siliplant ensured increase of seed productivity by 14-16%, mass of 100 seeds increased by 8-10%. Thus, by using complexes of humic preparations with growth regulators and silicon-containing microfertilization Siliplant as effective means of regulating the growth and development of medicinal cultures, activating flowering and seed formation processes, it is possible to ensure high yield and the best quality of the resulting seed material.
药用植物及其制剂广泛应用于多种疾病的治疗和预防。通过种植这些植物来满足制药工业对草药原料的需求。制药厂可以通过扩大种植面积和增加作物产量来充分供应药用原料,这是通过使用所需数量的有前途品种的优质种子来实现的。近年来,在VILAR北高加索分支药用作物轮作的西高加索条件下,对紫锥菊(Echinacea purpurea L.)、Сhamomilla recutta L.、车前草(Plantago major L.)和鼠尾草(Salvia officinalis L.)等在现代官方医学和传统医学用药中占有重要地位的植物种子产量的提高可能性进行了研究。为此,对腐殖质肥料(Lignogumat, Normat L)与生长调节剂(锆石,Agat, Gibbersib)和螯合微量肥料(sililplant和Cytovit)进行了复合试验。在紫锥菊上进行的研究表明,Lignogumat +玛瑙变种种子产量提高31%,鼠尾草和洋甘菊- Lignogumat +锆石变种种子产量提高28%,质量提高39%。千粒重比对照高8-15%。用Lignogumat和Normat L对紫锥菊和车前草进行无根饲养,含硅微施肥使其种子产量提高14-16%,百粒质量提高8-10%。因此,利用腐殖质制剂与生长调节剂的配合物和含硅微施肥硅藻土作为调节药用培养物生长发育、激活开花和种子形成过程的有效手段,可以保证种子材料的高产和最佳质量。
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引用次数: 0
The effectiveness of non-root fertilizers during cultivation white cabbage in the Non-Chernozem zone 在非切尔诺泽姆地区种植白甘蓝时使用非根肥的效果
Pub Date : 2023-12-04 DOI: 10.18619/2072-9146-2023-6-78-83
S. Nadezhkin, M. Markarova, M. Antoshkina, A. Molchanova, I. E. Osokin, O. Razin, A. Markarova
Relevance. Foliar feeding when growing vegetables is due to its positive effect on quality and yield indicators. The use of microfertilizers for foliar feeding allows optimizing the absorption of nutrients by plants, which can not only reduce economic costs, but also regulate the accumulation of biologically active substances. Identifying the optimal fertilizing system for different varieties and hybrids of vegetables is especially important in risky farming areas, where the main factor regulating yield indicators is weather.Methodology. The purpose of the work is to conduct a comparative assessment of the effectiveness of foliar feeding of the white cabbage variety Zimovka 1474 different agrochemicals. In a field experiment for three years (2018-2020), the effect of microfertilizers, humate, chelates and a biostimulator on the yield, biochemical and microelement composition of white cabbage variety Zimovka 1434 was studied.Results. It has been shown that the biometric characteristics of cabbage are best influenced by foliar fertilization with microfertilizer Aquarin and the biostimulator BIS-65, and the total and marketable yield is influenced by microfertilizer in chelate form Helaton. The greatest accumulation of dry matter, sugars, and vitamin C was typical for variants using the biological product BIS-65 and chelated fertilizers (Tiaton, Helaton). Chelated fertilizers also contributed to an increase in the accumulation of elements such as potassium, calcium, iron, zinc and manganese in cabbage heads.
的相关性。种植蔬菜时叶面取食是由于其对质量和产量指标的积极影响。利用微量肥进行叶面饲喂,可以优化植物对养分的吸收,不仅可以降低经济成本,还可以调节生物活性物质的积累。确定不同品种和杂交种蔬菜的最佳施肥制度在风险农业地区尤为重要,在这些地区,影响产量指标的主要因素是天气。本研究的目的是比较评价不同农用化学品对小白菜叶饲的效果。通过为期3年(2018-2020年)的田间试验,研究了微量肥、腐植酸盐、螯合剂和生物刺激剂对小白菜品种紫紫1434产量、生化及微量元素组成的影响。结果表明,叶面施用微肥Aquarin和生物刺激剂BIS-65对白菜的生物特征影响最大,螯合态微肥Helaton对白菜的总产量和适销产量影响最大。在使用生物制品BIS-65和螯合肥料的变异中,干物质、糖和维生素C的积累最多(Tiaton, Helaton)。螯合肥料还有助于增加白菜头中钾、钙、铁、锌和锰等元素的积累。
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引用次数: 0
Phenolic compounds in leaves of Tanacetum balsamita L. Tanacetum balsamita L. 叶子中的酚类化合物
Pub Date : 2023-12-04 DOI: 10.18619/2072-9146-2023-6-66-70
T. A. Krol, V. I. Ossipov, D. Baleev
Tanacetum balsamita L. is a perennial rhizomatous plant of the Asteraceae family. T. balsamita is cultivated as medicinal, aromatic plants and food plant. Extract of costmary exhibits hepatoprotective, antiseptic and anthelmintic properties. The herb of T. balsamita contains essential oils and phenolic compounds. This species included in the "Biocollection of medicinal and aromatic plants of open and protected ground", Botanical garden of the VILAR. The main aim was to study the composition of phenolic compounds in the leaves of T. balsamita with application of ultra-performance liquid chromatography coupled with diode array detection and mass spectrometry. The object of the study was the leaves of T. balsamita. Samples were extracted with 1 ml of 80% aqueous acetone. An ultra-performance liquid chromatographic system with a photodiode detector and a triple quadrupole mass spectrometer was used for analysis of phenolic compounds. The UV and MS data of phenolic compounds were used for their identification or tentative characterization with application of mass spectrometry databases and data published in the literature.Results. The results obtained showed the presence in the leaves of 17 phenolic compounds. Five compounds were identified as caffeoylquinic, feruloylquinic and three dicaffeolquinic acid isomers, and two compounds as ferulic acid hexoside isomers. The leaves of T. balsamita contained also five luteolin derivatives, two chrysoeriol derivatives, and spinacetin/axillarin. Four compounds were not identified.
香蒜是菊科多年生根茎植物。香茅是一种药用、芳香和食用植物。丹参提取物具有保肝、防腐、驱虫药的作用。香茅含有精油和酚类化合物。本种被列入“开放和保护区药用和芳香植物生物集锦”,VILAR植物园。采用超高效液相色谱-二极管阵列检测和质谱联用技术研究香田叶中酚类化合物的组成。本研究的对象是香椿的叶子。用1 ml 80%的丙酮水溶液提取样品。采用光电二极管检测器和三重四极杆质谱仪组成的超高效液相色谱系统对酚类化合物进行了分析。利用质谱数据库和文献发表的数据对酚类化合物进行鉴定或初步表征。得到的结果表明,在叶片中存在17种酚类化合物。鉴定出5个化合物为咖啡酰奎宁、阿魏酰奎宁和3个二咖啡酰奎宁酸异构体,2个化合物为阿魏酸己糖异构体。香茅叶中还含有5种木犀草素衍生物、2种黄蜡醇衍生物和spinacetin/axillarin。四种化合物未被鉴定。
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引用次数: 0
Comparative evaluation of biometrical and biochemical characteristics of three Rumex representatives: Rumex acetosa L., Rumex sanguineus L. and Rumex confertus Willd. 对三种鲁梅克斯代表植物的生物计量学和生物化学特征进行比较评估:Rumex acetosa L.、Rumex sanguineus L.和 Rumex confertus Willd.
Pub Date : 2023-12-04 DOI: 10.18619/2072-9146-2023-6-47-51
V. Kharchenko, N. A. Golubkina, M. N. Bogachuk
Increase in green vegetables popularity in nutrition and medicine promotes investigations of new cultivar and hybrid biochemical parameters. Nutritional value and antioxidant status of three Rumex representatives: Rumex acetosa L. (Krupnolistny cv.), Rumex sanguineus L. (prospect genotype) and Rumex confertus Willd. were studied. Antioxidant defense parameters (total antioxidant activity, polyphenol and ascorbic acid content) decreased in accordance to: Rumex sanguineus L. > Rumes confertus Willd. > Rumex acetosa L. Thus the ascorbic acid content in bloody dock (R. sanguineus)  reached 129 mg/100 g which was 1.48 times higher than in Russian dock (R. confertus) and 3.17 times higher than in garden sorrel (R. acetosa). The total chlorophyll content was also the highest in bloody dock (17.74 mg/g d.w.) compared to 15.88 mg/g in R. confertus and 13.07 mg/g in R. acetosa leaves. The new R. sanguineus genotype was characterized by high decorative properties and demonstrated twice higher oxalic acid content compared to R. acetosa, Krupnolistny cv., and 1.5 times higher value than in R. confertus. High decorative and medicinal value of R. sanguineus was connected to a great extent with the intensive anthocyanin accumulation reaching 7.2% and high nitrate content. The results of the present study indicate high prospects of R. sanguineus genotype utilization in landscape design, food industry and medicine.
绿色蔬菜在营养和医学上的普及促进了新品种和杂交种生化参数的研究。三种典型Rumex品种:Rumex acetosa L. (Krupnolistny cv.)、Rumex sanguineus L. (prospect基因型)和Rumex contus Willd的营养价值和抗氧化状况。进行了研究。抗氧化防御参数(总抗氧化活性、多酚和抗坏血酸含量)的降低顺序为:血尾瘤胃>野瘤胃。血船坞(R. sanguineus)中抗坏血酸含量达到129 mg/100 g,是俄罗斯船坞(R. confertus)的1.48倍,是酢浆草(R. acetosa)的3.17倍。总叶绿素含量也以血船坞最高(17.74 mg/g d.w.),而白菖蒲叶为15.88 mg/g,白菖蒲叶为13.07 mg/g。该新基因型具有较高的装饰性能,草酸含量比白沙、Krupnolistny等草酸含量高2倍。,比褐毛鼠高1.5倍。其花青素积累量高,达7.2%,硝酸盐含量高,具有很高的装饰和药用价值。本研究结果表明,该基因型在景观设计、食品工业和医药等方面具有广阔的应用前景。
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引用次数: 0
Nutrients and bioactive compounds of red cabbage 红甘蓝的营养成分和生物活性化合物
Pub Date : 2023-12-04 DOI: 10.18619/2072-9146-2023-6-84-89
A. Artemyeva, A. Solovyeva
The purpose of this study is to evaluate the biochemical components in the heads of some red cabbage accessions (Russian cultivar Mikhnevskaya, Russian hybrid from Co Sedek Promethey F1, Turkish variety Mohrenkopf, hybrids from the Netherlands Zomiro F1, Pecky F1, Remala F1, Japanese hybrid Pretino F1), during cultivation in the conditions of the Leningrad region. The content of dry matter, nutritional compounds of protein and sugars, biologically active pigments anthocyanins, chlorophylls, carotenoids, phenolic acids expressed in gallic acid equivalents, as well as the level of antioxidant activity using DPPH were determined. It was found that the content of most chemical components varied greatly between accessions, especially sugars, pigments, and phenolic compounds. As a result of metabolomic profiling, nine monosaccharides (84% of the total) and two disaccharides were identified, including five sugars common to all studied accessions, the predominant of which was fructose. The content of total chlorophylls in heads of cabbage was in the range of 0.48-50.43 mg/100 g (chlorophyll a was 70%), carotenoids in the range of 0.95-15.58 mg/100 g. Carotenoids included carotenes, represented mainly by βcarotene, luteins, violaxanthins, xanthophylls. The main anthocyanin in red cabbage accessions is cyanidin. A total of 19 components of the anthocyanin profile were identified; of which there are six main components, that account for 80% of the total. 11 phenolic compounds of various nature were identified, and all studied accessions contained quinic, ferulic, and sinapic acids. Sources of valuable biochemical traits have been found for breeding on quality for healthy nutrition: the cultivar Mohrenkopf stood out for its high content of dry substances; sugars – hybrid Zomiro F1; anthocyanins – hybrid Pecky F1; phenolic compounds – Pecky F1 and Mohrenkopf; high antioxidant activity - Mikhnevskaya, Mohrenkopf and Pecky F1.
本研究旨在评价在列宁格勒地区栽培条件下,俄罗斯品种Mikhnevskaya、俄罗斯Co Sedek杂交品种Promethey F1、土耳其品种Mohrenkopf、荷兰杂交品种Zomiro F1、Pecky F1、Remala F1、日本杂交品种Pretino F1等红白菜种头的生化成分。利用DPPH测定了干物质含量、蛋白质和糖的营养成分、生物活性色素花青素、叶绿素、类胡萝卜素、没食子酸当量中表达的酚酸含量以及抗氧化活性水平。结果表明,各品种间大部分化学成分的含量差异较大,尤其是糖、色素和酚类化合物。作为代谢组学分析的结果,鉴定出9种单糖(占总数的84%)和2种双糖,其中包括5种所有研究品种共有的糖,其中主要是果糖。甘蓝头总叶绿素含量为0.48 ~ 50.43 mg/100 g(叶绿素a含量为70%),类胡萝卜素含量为0.95 ~ 15.58 mg/100 g。类胡萝卜素包括胡萝卜素,主要有β胡萝卜素、叶黄素、紫黄素、叶黄素。红白菜中花青素的主要成分是花青素。共鉴定出花色苷谱的19个组分;其中有六个主要组成部分,占总数的80%。鉴定了11种不同性质的酚类化合物,所有被研究的材料都含有奎宁酸、阿魏酸和辛酸。为健康营养品质育种找到了有价值的生化性状来源:Mohrenkopf品种以其高干物质含量而突出;糖-杂化Zomiro F1;花青素-杂交Pecky F1;酚类化合物- Pecky F1和Mohrenkopf;高抗氧化活性- Mikhnevskaya, Mohrenkopf和Pecky F1。
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引用次数: 0
Mineral fertilizers for onions: a review 洋葱矿物肥料:综述
Pub Date : 2023-12-04 DOI: 10.18619/2072-9146-2023-6-52-60
O. N. Uspenskaya, A. Fedosov, A. Menshikh, I. Y. Vasyuchkov
For the production of onions, first of all, the proper organization of its nutrition is necessary. Both its productivity and product quality depend on this. First of all, onion nutrition requires mineral fertilizers containing macronutrients such as nitrogen, phosphorus and potassium. The quality of onions, their physiology and productivity depend on the application of nitrogen, phosphorus and potassium fertilizers, the need for which and the efficiency of use by plants vary depending on climatic factors, irrigation systems, varietal differences, soil and production conditions of cultivation in growing areas. This review examines in detail the effect of nitrogen, phosphorus and potassium, as the main components of mineral nutrition, on the growth, physiology and yield of onion. The review includes various databases such as Google Scholar, PubMed, Science Direct, SciFinder, Web of Science, RSCI, etc., online sources (Research Gate, Springer Nature Open Access, Wiley Online Library, etc.) are used.
对于洋葱的生产,首先,对其营养进行适当的组织是必要的。它的生产效率和产品质量都取决于此。首先,洋葱营养需要含有氮、磷、钾等常量营养素的矿质肥料。洋葱的品质、生理和生产力取决于氮、磷和钾肥的施用,而植物对氮、磷和钾肥的需求和使用效率因气候因素、灌溉系统、品种差异、土壤和种植区的生产条件而异。本文详细介绍了氮、磷、钾作为矿质营养的主要成分对洋葱生长、生理和产量的影响。本文收录了Google Scholar、PubMed、Science Direct、SciFinder、Web of Science、RSCI等数据库,并使用了Research Gate、Springer Nature Open Access、Wiley online Library等在线资源。
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引用次数: 0
The researches of broomrape acclimatization on white cabbage in the soil-climatic conditions of Central region of the Russian Federation 在俄罗斯联邦中部地区的土壤气候条件下对白甘蓝进行扫帚菜适应性研究
Pub Date : 2023-12-04 DOI: 10.18619/2072-9146-2023-6-95-100
B. M. Molokov, P. Y. Golysheva
Relevance. In one of the farms, situated in the Central region of the Russian Federation was found the first defeat of cabbage by obligate weed – broomrape. As the long experience shows (since 2011 yr.), the control of broomrape is very difficult, and it is a real danger for the vegetable growing in this region.Materials and methods. The object of researches were the plantations of white cabbage, which was grown by using the cassette technology, there were field experiments.The research methods included the following works: phenological observations of broomrape and white cabbage ontogenesis, the detection of the main features during blooming and fruiting of this weed, the definition of the duration of broomrape evolution until the fruiting phase, the detection of blooming shoots location on the surface of the cabbage plantation, the identifying of relationship between the height of broomrape’s flower-bearing shoots and the height of leaves of cabbage’s rosette.There was analyzed the feature of growing by using the cassette technology cabbage, being damaged by broomrape.Results. It was found, that the studied in the paper race of broomrape can successfully progress in more northern soil-climatic conditions of the Central region of the Russian Federation. The complex of agro technical methods of broomrape control is suggested, it includes: the two-level plowing of the weedy fields right after harvesting cabbage; receiving of cabbage’s fibrous root, which should be situated into the working plowing space with depth up to 25 cm; multiplex cultivation of the growing shoots of the weed; the cabbage hilling before rows closing. The alternative methods of broomrape control also can be used: the conveyer of cruciferous vegetable cultures with short vegetation period and cultures, which provoke the germination of the weed.
的相关性。在其中一个位于俄罗斯联邦中部地区的农场中,首次发现了白菜被专性杂草-扫帚菜击败的情况。长期的经验表明(自2011年以来),扫帚菜的控制非常困难,对该地区的蔬菜种植构成了真正的威胁。材料和方法。以盒式技术栽培的大白菜为研究对象,进行了大田试验。研究方法包括:对锦菜花和小白菜个体发生的物候观察,检测该杂草开花结果的主要特征,确定锦菜花进化至结果期的持续时间,检测花芽在大白菜地表的位置,确定锦菜花带花嫩芽高度与大白菜莲座叶高度的关系。分析了盒式栽培技术对甘蓝受飞天甘蓝危害的特点。研究结果表明,在俄罗斯联邦中部地区较北部的土壤气候条件下,毛茛纸种的研究可以成功地进行。提出了油菜防治的综合农业技术方法,包括:白菜收获后立即进行两层翻耕;接收卷心菜的纤维根,其应位于工作耕作空间,深度可达25厘米;杂草生长芽的复合栽培;白菜在收畦前隆起。此外,还可采用传送带种植期短的十字花科蔬菜和促进杂草萌发的栽培等防治方法。
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引用次数: 0
Pectin substances in Cucurbita maxima fruits in the conditions of the North-West of Russia 俄罗斯西北部地区葫芦果实中的果胶物质
Pub Date : 2023-12-04 DOI: 10.18619/2072-9146-2023-6-90-94
A. Solovyeva, T. M. Piskunova
Currently, pectins are widely used in the food, pharmaceutical industry, medicine and other industries due to the ability to form gels of various strengths, to show therapeutic and preventive properties. Cucurbita maxima pumpkin is considered as a source of natural and inexpensive pectin, as well as a functional food product for use in food and pharmaceutical preparations. The aim of the study was to identify the features of the accumulation of pectin substances in the most economically significant species of pumpkin C. maxima from the VIR collection. It was found that the studied varieties differ significantly in the amount of pectin substances. The content of pectins in the conditions of the North-West of Russia varied in the range from 0.59 to 4.24% (for absolutely dry matter). The content of soluble pectins was in the range of 0.24-1.36%. The level of protopectin accumulation, depending on the genotype and conditions of the growing season, was 0.35-3.37%. Sources of high content of soluble pectin and protopectin in pumpkin fruits have been identified for their further use in breeding, food and pharmaceutical industries.
目前,果胶由于能够形成各种强度的凝胶,表现出治疗和预防的特性,被广泛应用于食品、制药、医药等行业。南瓜被认为是天然和廉价的果胶的来源,以及用于食品和药物制剂的功能性食品产品。本研究的目的是确定VIR收集的最具经济意义的南瓜C. maxima物种中果胶物质积累的特征。结果表明,不同品种的果胶含量差异较大。在俄罗斯西北部条件下,果胶含量在0.59 ~ 4.24%(绝对干物质)之间变化。可溶性果胶含量在0.24 ~ 1.36%之间。不同基因型和生长季节条件下,原粘连素积累水平为0.35 ~ 3.37%。在南瓜果实中发现了高含量的可溶性果胶和原果胶的来源,可进一步用于育种、食品和制药工业。
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引用次数: 0
Efficiency of PCR marker F311 of the SGR gene in assessing the resistance of cucumber lines to downy mildew Pseudoperonospora cubensis SGR 基因的 PCR 标记 F311 在评估黄瓜品系对立方假孢子菌霜霉病的抗性时的效率
Pub Date : 2023-12-03 DOI: 10.18619/2072-9146-2023-6-11-16
D. D. Teplyakova
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引用次数: 0
Molecular and genetic basis of tomato resistance to major fungal diseases 番茄抵抗主要真菌病害的分子和遗传基础
Pub Date : 2023-12-03 DOI: 10.18619/2072-9146-2023-6-28-39
M. V. Maslova, I. Shamshin, E. Grosheva, A. S. Ilyichev
This review presents an analysis of the literature, which indicates the popularity of breeding methods in the fight against oomycete and fungal diseases of tomato: late blight (pathogen − Phytophthora infestans (Mont.) de Bary), fusarium wilt (pathogen − Fusarium oxysporum f. sp. lycopersici (Sacc.) Snyder and Hansen), early blight or alternariosis (pathogens − representatives of the genus Alternaria), cladosporiosis (pathogen−Cladosporium fulvum Cook.). Theoretical and practical achievements, the latest developments in genetics, molecular biology, plant physiology, microbiology are considered. Special attention is paid to modern information about the diversity of tomato pathogens, their morphological, genetic, physiological features, including racial composition; the presence of pathogen resistance genes in the gene pool of the culture under study, their mapping and the possibility of introduction into the genome from other sources; about the developed markers of target genes and loci of quantitative traits; the degree of associations between a molecular marker and a target gene; about the features of inheritance of the studied trait, as well as the possibility of pyramiding R-genes and QTL in one genotype. To accelerate and improve the efficiency of tomato breeding for resistance to pathogens, these issues are of great importance. Their study will strengthen the integration of modern biotechnology with the traditional breeding process, which is carried out by classical methods.
本文综述了番茄卵菌和真菌病害的育种方法,包括晚疫病(病原菌-疫霉疫霉(Mont.) de Bary)、枯萎病(病原菌-镰刀菌f. sp. lycopersici (Sacc.))。Snyder和Hansen),早期枯萎病或交替病(病原体-交替属的代表),枝孢子虫病(病原体- Cladosporium fulvum Cook)。理论和实践成果,遗传学,分子生物学,植物生理学,微生物学的最新发展。特别关注关于番茄病原体多样性的现代信息,包括其形态、遗传、生理特征,包括种族组成;在所研究的培养物基因库中是否存在病原菌抗性基因,它们的定位以及从其他来源引入基因组的可能性;目的基因标记和数量性状位点的发育情况关联度:分子标记与靶基因之间的关联度;探讨了所研究性状的遗传特征,以及在一个基因型中存在r基因和QTL的可能性。这些问题对加快番茄抗病育种和提高番茄抗病育种效率具有重要意义。他们的研究将加强现代生物技术与传统育种过程的结合,传统育种过程是通过经典方法进行的。
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引用次数: 0
期刊
Vegetable crops of Russia
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