Pub Date : 2022-09-30DOI: 10.15625/1811-4989/16835
L. T. Thơm, Nguyen Cam Ha, H. M. Hien, N. H. Thu, V. Loan, Nguyen Trong Dan, Truong Ba Hai, D. D. Hong
The sea area of the Truong Sa Archipelago, Vietnam - outpost island region of the country has a special geographical location and plays an important role in economic development as well as security and defense of Vietnam. Here, the first time for isolation of heterotrophic marine microalgae is precious. In this paper, the results on isolation, identification, and selection of suitable culture conditions of the strain Thraustochytrium pachydermum TSL10 isolated from the sea area of Spratly Island, Truong Sa Archipelago, Vietnam in 2021 were presented. The optimal culture conditions for the growth of T. pachydermum TSL10 in the flask were M1 medium, 3% glucose, 1% yeast extract and 25 - 28 °C temperature. Dry biomass and lipid content reached the highest levels of 12.54 ± 1.23 g/L and 28.65 ± 1.11 % dry cell weight (DCW), respectively. In a 30 L fermentor, T. pachydermum TSL10 grew well in a medium containing 9% glucose and 1% industrial extract yeast. After 120 hours of cultivation, dry biomass, lipid, protein, carbohydrate, carotenoids, and astaxanthin reached the highest levels of 30.76 ± 1.32 g/L; 22.95 ± 0.28% DCW; 6.65 ± 0.19% DCW, 15.96 ± 0.021% DCW, 178.00 ± 2.43 µg/L, and 8.30 ± 0.28 µg/L, respectively. The fatty acid composition contains mainly omega - 6 and omega - 9 fatty acids, accounting for 21.69% of total fatty acids (TFA) and 34.56% of TFA. The fatty acid profile was mainly dominated by linoleic acid and oleic acid, accounting for 21.54% of TFA and 34.23% of TFA, respectively. The results showed that TSL10 biomass was suitable raw material for screening bioactive compounds.
{"title":"Biological characteristics of the heterotrophic marine microalgae Thraustochytrium pachydermum TSL10 isolated from the sea area of Truong Sa Archipelago, Vietnam","authors":"L. T. Thơm, Nguyen Cam Ha, H. M. Hien, N. H. Thu, V. Loan, Nguyen Trong Dan, Truong Ba Hai, D. D. Hong","doi":"10.15625/1811-4989/16835","DOIUrl":"https://doi.org/10.15625/1811-4989/16835","url":null,"abstract":"The sea area of the Truong Sa Archipelago, Vietnam - outpost island region of the country has a special geographical location and plays an important role in economic development as well as security and defense of Vietnam. Here, the first time for isolation of heterotrophic marine microalgae is precious. In this paper, the results on isolation, identification, and selection of suitable culture conditions of the strain Thraustochytrium pachydermum TSL10 isolated from the sea area of Spratly Island, Truong Sa Archipelago, Vietnam in 2021 were presented. The optimal culture conditions for the growth of T. pachydermum TSL10 in the flask were M1 medium, 3% glucose, 1% yeast extract and 25 - 28 °C temperature. Dry biomass and lipid content reached the highest levels of 12.54 ± 1.23 g/L and 28.65 ± 1.11 % dry cell weight (DCW), respectively. In a 30 L fermentor, T. pachydermum TSL10 grew well in a medium containing 9% glucose and 1% industrial extract yeast. After 120 hours of cultivation, dry biomass, lipid, protein, carbohydrate, carotenoids, and astaxanthin reached the highest levels of 30.76 ± 1.32 g/L; 22.95 ± 0.28% DCW; 6.65 ± 0.19% DCW, 15.96 ± 0.021% DCW, 178.00 ± 2.43 µg/L, and 8.30 ± 0.28 µg/L, respectively. The fatty acid composition contains mainly omega - 6 and omega - 9 fatty acids, accounting for 21.69% of total fatty acids (TFA) and 34.56% of TFA. The fatty acid profile was mainly dominated by linoleic acid and oleic acid, accounting for 21.54% of TFA and 34.23% of TFA, respectively. The results showed that TSL10 biomass was suitable raw material for screening bioactive compounds.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":" 28","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91410839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.15625/1811-4989/16673
Le Tho Son, Nguyen Hai Dang, Lai Thi Phuong, Nguyen Thi Thu, Nguyen Huy Hoang
Dicarbonyl/L-xylulose reductase (DCXR) is an enzyme reducing dicarbonyl (-CO-) groups in single sugar molecules and participates in the sugar metabolism of several sugar metabolism cycles in different living organisms (humans, other animals, fungi, and microorganisms). Caenorhabditis elegans, a model organism, has a unique DCXR (referred to as Ce DCXR) which plays a biochemical function similar to its homologs in the other organisms. However, the catalytic regulation of the enzyme has not been fully elucidated yet. Therefore, in this study, we investigated the chemicals which could inhibit the enzyme and found three inhibitor compounds including hexanoic acid, phosphoenolpyruvic acid, and DL-α-aminobutyrate hydrochloride for the enzyme. Because these inhibitors and their derivatives suppressed DCXRs in humans, mice, rabbits, and rats, they possibly inhibit the DCXR homologs at least in other animals. If the inhibitors and their derivatives are components in pharmaceutical products, foods, and drinks, they may come into cells, interact with DCXRs and inactivate them, causing toxicity for the host organisms.
二羰基/ l -木糖还原酶(Dicarbonyl/L-xylulose reductase, DCXR)是一种还原单糖分子中二羰基(- co -)基团的酶，在不同的生物体(人类、其他动物、真菌和微生物)中参与几个糖代谢循环的糖代谢。秀丽隐杆线虫是一种模式生物，具有独特的DCXR(简称Ce DCXR)，其生化功能与其在其他生物中的同源物相似。然而，该酶的催化调控作用尚未完全阐明。因此，在本研究中，我们对抑制该酶的化学物质进行了研究，发现了己酸、磷酸烯醇丙酮酸和DL-α-氨基丁酸盐酸盐三种抑制该酶的化合物。由于这些抑制剂及其衍生物在人、小鼠、家兔和大鼠中抑制DCXR，它们可能至少在其他动物中抑制DCXR同源物。如果抑制剂及其衍生物是药品、食品和饮料中的成分，它们可能会进入细胞，与dcxr相互作用并使其失活，从而对宿主生物造成毒性。
{"title":"New inhibitors for dicarbonyl/L-xylulose reductase in Caenorhabditis elegans","authors":"Le Tho Son, Nguyen Hai Dang, Lai Thi Phuong, Nguyen Thi Thu, Nguyen Huy Hoang","doi":"10.15625/1811-4989/16673","DOIUrl":"https://doi.org/10.15625/1811-4989/16673","url":null,"abstract":"Dicarbonyl/L-xylulose reductase (DCXR) is an enzyme reducing dicarbonyl (-CO-) groups in single sugar molecules and participates in the sugar metabolism of several sugar metabolism cycles in different living organisms (humans, other animals, fungi, and microorganisms). Caenorhabditis elegans, a model organism, has a unique DCXR (referred to as Ce DCXR) which plays a biochemical function similar to its homologs in the other organisms. However, the catalytic regulation of the enzyme has not been fully elucidated yet. Therefore, in this study, we investigated the chemicals which could inhibit the enzyme and found three inhibitor compounds including hexanoic acid, phosphoenolpyruvic acid, and DL-α-aminobutyrate hydrochloride for the enzyme. Because these inhibitors and their derivatives suppressed DCXRs in humans, mice, rabbits, and rats, they possibly inhibit the DCXR homologs at least in other animals. If the inhibitors and their derivatives are components in pharmaceutical products, foods, and drinks, they may come into cells, interact with DCXRs and inactivate them, causing toxicity for the host organisms.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89027426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.15625/1811-4989/16571
Nguyen Thi Nguyet, Tran Cam Van, Nguyen Thi Xuan Thu, Nguyen Thi Mai Phuong
Trichophyton rubrum is a causative agent responsible for human chronic dermatophytosis worlwide. It is a common causative agent of the dermatophyte infections and resistant to many antifungal drugs. Oxidative damaging agents belong to a strong antimicrobial group and have been intensively investigated. The synergistic oxidative damage can occur if the agents react with metal cations. Therefore, in this study the antifungal activity of formulas containing the two oxidative damaging agents, including hydrogen peroxide (H2O2) and 8-hydroxyquinoline (8HQ), in combination with metal cations has been determined to examine the synergistic damage in T. rubrum isolated from the skin of a dermatological patient. The isolate was identified by morphology observations, biochemical tests and sequence analysis of ITS1-4 gene, in comparison with the similar ITS nucleotide sequences of others. The obtained results showed that the isolated fungal strain had physiological and biochemical characteristics of T. rubrum with more than 99% identity of the T. rubrum CBS 392.58 ITS sequence with OL347577 accession numbers. The antifungal tests indicated that the combinations of 2 mM Cu2+ + 0.05% H2O2; 2 mM Cu2++ 0.005% 8HQ; 2 mM Cu2+ + 0.005% 8HQ + 0.05% H2O2 exerted the best antifungal activity against the isolated T. rubrum with the diameter of inhibition zones of 22, 25 and 28 mm, respectively. Among the combinations containing Zn2+, the only formula of 2 mM Zn2+ + 0.05% H2O2 exhibited the desired effect, while the combinations containing Fe2+ did not show any activity. Thus, our study suggested a potential use of Cu2+ + 0.05% H2O2; 2 mM Cu2++ 0.005% 8HQ and 2 mM Cu2+ + 0.005% 8HQ + 0.05% H2O2 formulas as new potential antifungal agents against T. rubrum due to their synergistic oxidative damage.
在世界范围内，红毛癣菌是引起人类慢性皮肤病的病原体。它是皮肤真菌感染的常见病原体，对许多抗真菌药物具有耐药性。氧化损伤剂是一种强抗菌药物，目前已被广泛研究。与金属阳离子反应可产生协同氧化损伤。因此，在本研究中，我们测定了含有过氧化氢(H2O2)和8-羟基喹啉(8HQ)两种氧化损伤剂的配方与金属阳离子结合的抗真菌活性，以检测从皮肤病患者皮肤分离的红毛霉的协同损伤。通过形态学观察、生化试验和ITS - 1-4基因序列分析，并与其他相似ITS核苷酸序列进行比较，对分离物进行鉴定。结果表明，分离得到的真菌菌株具有红血球的生理生化特征，与编号为OL347577的红血球CBS 392.58 ITS序列的一致性在99%以上。抑菌试验结果表明:2 mM Cu2+ 0.05% H2O2组合;2 mM Cu2++ 0.005% 8HQ;2 mM Cu2+ + 0.005% 8HQ + 0.05% H2O2抑菌效果最佳，抑菌带直径分别为22、25和28 mM。在含Zn2+的组合中，只有2 mM Zn2+ + 0.05% H2O2的组合具有理想的效果，而含Fe2+的组合没有表现出任何活性。因此，我们的研究建议使用Cu2+ + 0.05% H2O2;2 mM Cu2++ 0.005% 8HQ和2 mM Cu2++ 0.005% 8HQ + 0.05% H2O2配方具有协同氧化损伤作用，是一种潜在的抗真菌药物。
{"title":"Antifungal activity of oxidative damaging agents in combination with metal cations against Trichophyton rubrum isolated from a dermatological patient","authors":"Nguyen Thi Nguyet, Tran Cam Van, Nguyen Thi Xuan Thu, Nguyen Thi Mai Phuong","doi":"10.15625/1811-4989/16571","DOIUrl":"https://doi.org/10.15625/1811-4989/16571","url":null,"abstract":"Trichophyton rubrum is a causative agent responsible for human chronic dermatophytosis worlwide. It is a common causative agent of the dermatophyte infections and resistant to many antifungal drugs. Oxidative damaging agents belong to a strong antimicrobial group and have been intensively investigated. The synergistic oxidative damage can occur if the agents react with metal cations. Therefore, in this study the antifungal activity of formulas containing the two oxidative damaging agents, including hydrogen peroxide (H2O2) and 8-hydroxyquinoline (8HQ), in combination with metal cations has been determined to examine the synergistic damage in T. rubrum isolated from the skin of a dermatological patient. The isolate was identified by morphology observations, biochemical tests and sequence analysis of ITS1-4 gene, in comparison with the similar ITS nucleotide sequences of others. The obtained results showed that the isolated fungal strain had physiological and biochemical characteristics of T. rubrum with more than 99% identity of the T. rubrum CBS 392.58 ITS sequence with OL347577 accession numbers. The antifungal tests indicated that the combinations of 2 mM Cu2+ + 0.05% H2O2; 2 mM Cu2++ 0.005% 8HQ; 2 mM Cu2+ + 0.005% 8HQ + 0.05% H2O2 exerted the best antifungal activity against the isolated T. rubrum with the diameter of inhibition zones of 22, 25 and 28 mm, respectively. Among the combinations containing Zn2+, the only formula of 2 mM Zn2+ + 0.05% H2O2 exhibited the desired effect, while the combinations containing Fe2+ did not show any activity. Thus, our study suggested a potential use of Cu2+ + 0.05% H2O2; 2 mM Cu2++ 0.005% 8HQ and 2 mM Cu2+ + 0.005% 8HQ + 0.05% H2O2 formulas as new potential antifungal agents against T. rubrum due to their synergistic oxidative damage.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82230448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.15625/1811-4989/17059
Le Thi Van Anh, Tran Ngoc Anh Thu, Nguyen Thi Dong Phuong
Harvesting of microalgae from their different cultivation media has pointed out challenges in resolving the problems of flocculation. These challenges must be faced with a suitable method for inducing flocculation that avoid or limit the microalgae’s contamination. This study developed the fundamental experiments with a support of chemicals and some bacteria strains inducing the flocculation of Chlorella vulgaris SAG 211-19. Particularly, the determination of minimum content of Mg2+, Ca2+, E. coli ATCC 85922 and Bacillus subtilis MT300405 was effectuated with co-cultivation of microalgae and set up in batch culture in Bold’s Basal Medium. As a result, the adjustment in 25 minutes of 199.2 mg/L CaCl2.2H2O, 50 mg/L KH2PO4, and of 141 mg/L MgSO4.7H2O induced a microalgal settling efficiency of 81% and 70%, respectively. Meanwhile, the perfomance of microalgal removing reached up to 83.6% and 84% by the inoculation into microalgal culture media of a minimum initial cell density of 8.1 ´ 105 CFU/mL of Bacillus subtilis MT300405 and 12 ´ 105 CFU/mL of E. coli ATCC 85922, respectively. The flocculation of microalgal cells by bacterial inoculation did not require a high pH adjustment as in the case of salt addition.
{"title":"Investigation of microalgae culture by autoflocculation methodologies","authors":"Le Thi Van Anh, Tran Ngoc Anh Thu, Nguyen Thi Dong Phuong","doi":"10.15625/1811-4989/17059","DOIUrl":"https://doi.org/10.15625/1811-4989/17059","url":null,"abstract":"Harvesting of microalgae from their different cultivation media has pointed out challenges in resolving the problems of flocculation. These challenges must be faced with a suitable method for inducing flocculation that avoid or limit the microalgae’s contamination. This study developed the fundamental experiments with a support of chemicals and some bacteria strains inducing the flocculation of Chlorella vulgaris SAG 211-19. Particularly, the determination of minimum content of Mg2+, Ca2+, E. coli ATCC 85922 and Bacillus subtilis MT300405 was effectuated with co-cultivation of microalgae and set up in batch culture in Bold’s Basal Medium. As a result, the adjustment in 25 minutes of 199.2 mg/L CaCl2.2H2O, 50 mg/L KH2PO4, and of 141 mg/L MgSO4.7H2O induced a microalgal settling efficiency of 81% and 70%, respectively. Meanwhile, the perfomance of microalgal removing reached up to 83.6% and 84% by the inoculation into microalgal culture media of a minimum initial cell density of 8.1 ´ 105 CFU/mL of Bacillus subtilis MT300405 and 12 ´ 105 CFU/mL of E. coli ATCC 85922, respectively. The flocculation of microalgal cells by bacterial inoculation did not require a high pH adjustment as in the case of salt addition.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87421663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.15625/1811-4989/16585
Huong Le, Hoang-Nghi Mai-Thi, X. Le, N. Tran, Cam Tu Tran, Khon Huynh
In tissue engineering, coating biomaterial’s surface with extracellular matrix (ECM) proteins can promote many biological activities, including cellular responses, wound healing, and rejection reduction. Several interactions occur upon binding proteins onto the surfaces, leading to changes in the protein structural conformation, directly affecting the cell-host interactions. Therefore, this study investigates the impacts of surfaces’ wettability on protein conformation. In order to get the insights, organosilicate (OGS) was utilized to modify the tissue culture plate, resulting in surfaces with different wettability, followed by fibronectin (FN) immobilization. Then, the surfaces were used to study the fibrinogen interaction, cell attachment, and spreading. The results showed that OGS-modified surfaces produced four different wettability, ranging from super-hydrophilic (OGS150), hydrophilic (OGS100), hydrophobic (OGS60), to super-hydrophobic (OGS5). Each surface possessed particular nature, resulting in the variation of FN molecules' structural change. The amount of FN adsorbed on the OGS-coated surfaces was shown not to be perfectly proportional to the results of fibrinogen interaction, cell attachment, and spreading. The super-hydrophobic surfaces (OGS5) were highest in the amount of immobilized FN and the efficiency in subsequent experiments among the OGS-coated surfaces group. Notably, the hydrophobic surface adsorbed the lowest amount of FN but achieved remarkable results in the following experiments. Thus, this study holds a promising potential in producing biocompatible materials in tissue engineering.
{"title":"The concentration-independence cellular effects of fibronectin adsorbed on material surfaces with different hydrophobicities","authors":"Huong Le, Hoang-Nghi Mai-Thi, X. Le, N. Tran, Cam Tu Tran, Khon Huynh","doi":"10.15625/1811-4989/16585","DOIUrl":"https://doi.org/10.15625/1811-4989/16585","url":null,"abstract":"In tissue engineering, coating biomaterial’s surface with extracellular matrix (ECM) proteins can promote many biological activities, including cellular responses, wound healing, and rejection reduction. Several interactions occur upon binding proteins onto the surfaces, leading to changes in the protein structural conformation, directly affecting the cell-host interactions. Therefore, this study investigates the impacts of surfaces’ wettability on protein conformation. In order to get the insights, organosilicate (OGS) was utilized to modify the tissue culture plate, resulting in surfaces with different wettability, followed by fibronectin (FN) immobilization. Then, the surfaces were used to study the fibrinogen interaction, cell attachment, and spreading. The results showed that OGS-modified surfaces produced four different wettability, ranging from super-hydrophilic (OGS150), hydrophilic (OGS100), hydrophobic (OGS60), to super-hydrophobic (OGS5). Each surface possessed particular nature, resulting in the variation of FN molecules' structural change. The amount of FN adsorbed on the OGS-coated surfaces was shown not to be perfectly proportional to the results of fibrinogen interaction, cell attachment, and spreading. The super-hydrophobic surfaces (OGS5) were highest in the amount of immobilized FN and the efficiency in subsequent experiments among the OGS-coated surfaces group. Notably, the hydrophobic surface adsorbed the lowest amount of FN but achieved remarkable results in the following experiments. Thus, this study holds a promising potential in producing biocompatible materials in tissue engineering.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"270 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91446969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.15625/1811-4989/16428
Nguyen Thi Thanh Huyen, Nguyen Hoang Giang, Nguyen Thi Xuan
Myeloid leukemia (ML) is a cancer of the blood that begins when cells of the myeloid lineage uncontrollably change and grow. Acute myeloid leukemia (AML) is a disorder of rapid, uncontrolled growth of immature myeloid cells in the blood and bone marrow. Chronic myeloid leukemia (CML) is characterized by the aberrant proliferation of myeloid cells and driven by the translocation of regions of the BCR and ABL genes to form the Philadelphia (Ph) chromosome. The deubiquitinase enzymes (DUBs) including A20, OTUB1, OTUB2, and Cezanne play important roles in inhibiting NF-κB activation in response to various stimuli. Cytokines including tumor necrosis factor-alpha (TNF-α), IL-6, and IL-1β are released from immune cell activation triggered by antigenic stimulation. To this end, blood samples of 20 AML and 62 CML patients and the control group consisting of 37 healthy individuals were used to examine the mRNA expression of A20, OTUB1, OTUB2 and Cezanne genes by using quantitative RT-PCR and determine IL-6, TNF-α and IL-1β concentrations by using ELISA. As a result, the mRNA level of OTUB1 was significantly decreased in both AML and CML patients compared to that in healthy individuals, however, no difference in the transcriptional expression of OTUB2 among AML and CML patients and control group was detected. Unlike the levels of OTUB1 and OTUB2, the expressions of A20 and Cezanne in CML, but not in AML patients were significantly lower than healthy individuals. For serum cytokine analysis of the study groups, in AML and CML samples, IL-6 and TNF-α concentrations significantly increased in comparison with the control group, however, IL-1β level was similar among CML, AML patients and healthy individuals. In conclusion, this study revealed the different DUB involvement in the pathogenesis of ML, suggesting further investigations on gene polymorphisms and their functions linked to biological properties of leukemia cells.
{"title":"Expression of deubiquitinase genes and inflammatory response in myeloid leukemia","authors":"Nguyen Thi Thanh Huyen, Nguyen Hoang Giang, Nguyen Thi Xuan","doi":"10.15625/1811-4989/16428","DOIUrl":"https://doi.org/10.15625/1811-4989/16428","url":null,"abstract":"Myeloid leukemia (ML) is a cancer of the blood that begins when cells of the myeloid lineage uncontrollably change and grow. Acute myeloid leukemia (AML) is a disorder of rapid, uncontrolled growth of immature myeloid cells in the blood and bone marrow. Chronic myeloid leukemia (CML) is characterized by the aberrant proliferation of myeloid cells and driven by the translocation of regions of the BCR and ABL genes to form the Philadelphia (Ph) chromosome. The deubiquitinase enzymes (DUBs) including A20, OTUB1, OTUB2, and Cezanne play important roles in inhibiting NF-κB activation in response to various stimuli. Cytokines including tumor necrosis factor-alpha (TNF-α), IL-6, and IL-1β are released from immune cell activation triggered by antigenic stimulation. To this end, blood samples of 20 AML and 62 CML patients and the control group consisting of 37 healthy individuals were used to examine the mRNA expression of A20, OTUB1, OTUB2 and Cezanne genes by using quantitative RT-PCR and determine IL-6, TNF-α and IL-1β concentrations by using ELISA. As a result, the mRNA level of OTUB1 was significantly decreased in both AML and CML patients compared to that in healthy individuals, however, no difference in the transcriptional expression of OTUB2 among AML and CML patients and control group was detected. Unlike the levels of OTUB1 and OTUB2, the expressions of A20 and Cezanne in CML, but not in AML patients were significantly lower than healthy individuals. For serum cytokine analysis of the study groups, in AML and CML samples, IL-6 and TNF-α concentrations significantly increased in comparison with the control group, however, IL-1β level was similar among CML, AML patients and healthy individuals. In conclusion, this study revealed the different DUB involvement in the pathogenesis of ML, suggesting further investigations on gene polymorphisms and their functions linked to biological properties of leukemia cells.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76614158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.15625/1811-4989/15819
Tran Thi Ngoc Ha, Hoang Van Duong, Nguyen Huynh Cam Tu, Phan Tuong Loc
We have known that plant defensins have the main roles in biotic stress, especially antifungal activities. In addition, their functions on abiotic stress tolerance have been demonstrated on previous studies, but that information was still limited. Ca-AFP, a plant defensin gene derived from chickpea, was transformed into tobacco leaf disks by Agrobacterium method. After 16 weeks on selection media containing 15 mg/L hygromycin, some regeneration shoot lines were obtained. Four of them showed the positive bands on hpt gene and Ca-AFP gene by PCR screening. These lines were applied on in vitro evaluation of dehydration tolerance using rotary liquid culture, and the water-deficit stress was caused by 2% (w/v) PEG 6000. These four transgenic lines had better growth of adventitious roots (number of adventitious roots, total adventitious root length, and average of root length) and higher rate of biomass increase than the non-transgenic line. The result illustrated that the expression of Ca-AFP gene could mitigate the negative effect of water-deficit stress on transgenic tobacco lines under the in vitro stress condition. These transgenic tobacco lines are potential for water-deficit stress trials on greenhouse in the future.
{"title":"Agrobacterium-mediated transformation and in vitro evaluation of dehydration tolerance in transgenic tobacco harboring Ca-AFP gene","authors":"Tran Thi Ngoc Ha, Hoang Van Duong, Nguyen Huynh Cam Tu, Phan Tuong Loc","doi":"10.15625/1811-4989/15819","DOIUrl":"https://doi.org/10.15625/1811-4989/15819","url":null,"abstract":"We have known that plant defensins have the main roles in biotic stress, especially antifungal activities. In addition, their functions on abiotic stress tolerance have been demonstrated on previous studies, but that information was still limited. Ca-AFP, a plant defensin gene derived from chickpea, was transformed into tobacco leaf disks by Agrobacterium method. After 16 weeks on selection media containing 15 mg/L hygromycin, some regeneration shoot lines were obtained. Four of them showed the positive bands on hpt gene and Ca-AFP gene by PCR screening. These lines were applied on in vitro evaluation of dehydration tolerance using rotary liquid culture, and the water-deficit stress was caused by 2% (w/v) PEG 6000. These four transgenic lines had better growth of adventitious roots (number of adventitious roots, total adventitious root length, and average of root length) and higher rate of biomass increase than the non-transgenic line. The result illustrated that the expression of Ca-AFP gene could mitigate the negative effect of water-deficit stress on transgenic tobacco lines under the in vitro stress condition. These transgenic tobacco lines are potential for water-deficit stress trials on greenhouse in the future.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"79 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76064818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.15625/1811-4989/16478
Nguyen Phan Lan Hong, Nguyen Minh Duc, Nguyen Minh Tam
Anisoptera costata is an endangered species in Vietnam, from habitat loss and over-exploitation. To protect this species, we analyzed 56 adult trees using eight microsatellites to investigate the genetic diversity within and among three A. costata populations, representing the range of distribution of this species in southeastern Vietnam. Our results showed low genetic diversity (NA = 2.5, HO = 0.244, HE = 0.268). Genetic population differentiation among populations was low (FST = 0.139), indicating high gene flow (Nm = 2.838). Genetic variation among individuals was 79.77%. These suggest a lack of genetic diversity in A. costata. The clustering analysis also showed two genetic clusters. These results will provide necessary information to improve the efficiency of conservation and management of this species.
由于栖息地丧失和过度开发，越南无翅目是一种濒危物种。为了保护这一物种，我们利用8颗微卫星对56棵成树进行了遗传多样性的分析，研究了3个居群间的遗传多样性，代表了该物种在越南东南部的分布范围。遗传多样性低(NA = 2.5, HO = 0.244, HE = 0.268)。群体间的遗传分化程度较低(FST = 0.139)，表明存在较高的基因流(Nm = 2.838)。个体间遗传变异率为79.77%。这些结果表明，a. costata缺乏遗传多样性。聚类分析也显示出两个遗传聚类。这些结果将为提高该物种的保护和管理效率提供必要的信息。
{"title":"Genetic structure of Anisoptera costata in Southeast Vietnam","authors":"Nguyen Phan Lan Hong, Nguyen Minh Duc, Nguyen Minh Tam","doi":"10.15625/1811-4989/16478","DOIUrl":"https://doi.org/10.15625/1811-4989/16478","url":null,"abstract":"Anisoptera costata is an endangered species in Vietnam, from habitat loss and over-exploitation. To protect this species, we analyzed 56 adult trees using eight microsatellites to investigate the genetic diversity within and among three A. costata populations, representing the range of distribution of this species in southeastern Vietnam. Our results showed low genetic diversity (NA = 2.5, HO = 0.244, HE = 0.268). Genetic population differentiation among populations was low (FST = 0.139), indicating high gene flow (Nm = 2.838). Genetic variation among individuals was 79.77%. These suggest a lack of genetic diversity in A. costata. The clustering analysis also showed two genetic clusters. These results will provide necessary information to improve the efficiency of conservation and management of this species.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"92 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74082711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.15625/1811-4989/16043
Nguyen Khac Hung, Do Thi Gam, Hoang Thi Huyen Trang, Chu Hoang Ha, Do Tien Phat, Pham Bich Ngoc
Light quality is known to affects numerous plants’ physiological and metabolic processes during development period, including photosynthesis and morphogenesis. Light properties such as light wavelength has been optimized for several cultivated crops. In this study, the effects of LED light wavelengths blue and red on both photosynthetic performance and morphology of Gerbera jamesonii plantlets was investigated. The results showed the photosynthesis efficiency via the photo-pigment system and mediated regulation of rubisco activase encoding gene (RCA) mechanism was significantly enhanced by blue light. The expression of RCA was approximatly 2.7 times higher than those under red light. In contrast, chlorophyll contents, CO2 assimilation, total carbohydrate and RCA expression was reduced under red wavelength. However, both single-wavelengths caused a dramatically negative impact on G. jamesonii. Total chlorophyll/carotenoid value or higher carotenoid contents was lower under blue light; fragile petioles and drawrfism morphology was occurred under red and blue LED, respectively. The combination of blue and red light in the lighting spectrum significantly improved the limitations of single-wavelength. The supplement of blue light under red light background (at blue LED and red LED ratio = 1:4) improved plant photosynthesis while maintained the normal morphology of plants, although the expression of RCA and net photosynthesis of plants in BR LED was lower than in the control. Our results obviously provide the scientific evidences for requirement of LED light in micropropagation or canopy culture of G. jamesonii.
{"title":"Effects of blue and red LED lights on rubisco activase gene expression, CO2 fixation efficiency and plant morphology of Gerbera jamesonii","authors":"Nguyen Khac Hung, Do Thi Gam, Hoang Thi Huyen Trang, Chu Hoang Ha, Do Tien Phat, Pham Bich Ngoc","doi":"10.15625/1811-4989/16043","DOIUrl":"https://doi.org/10.15625/1811-4989/16043","url":null,"abstract":"Light quality is known to affects numerous plants’ physiological and metabolic processes during development period, including photosynthesis and morphogenesis. Light properties such as light wavelength has been optimized for several cultivated crops. In this study, the effects of LED light wavelengths blue and red on both photosynthetic performance and morphology of Gerbera jamesonii plantlets was investigated. The results showed the photosynthesis efficiency via the photo-pigment system and mediated regulation of rubisco activase encoding gene (RCA) mechanism was significantly enhanced by blue light. The expression of RCA was approximatly 2.7 times higher than those under red light. In contrast, chlorophyll contents, CO2 assimilation, total carbohydrate and RCA expression was reduced under red wavelength. However, both single-wavelengths caused a dramatically negative impact on G. jamesonii. Total chlorophyll/carotenoid value or higher carotenoid contents was lower under blue light; fragile petioles and drawrfism morphology was occurred under red and blue LED, respectively. The combination of blue and red light in the lighting spectrum significantly improved the limitations of single-wavelength. The supplement of blue light under red light background (at blue LED and red LED ratio = 1:4) improved plant photosynthesis while maintained the normal morphology of plants, although the expression of RCA and net photosynthesis of plants in BR LED was lower than in the control. Our results obviously provide the scientific evidences for requirement of LED light in micropropagation or canopy culture of G. jamesonii.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"136 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77456848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.15625/1811-4989/16604
Hoang Thi Quynh, Duong Thi Thuy, Doan Thi Oanh, Nguyen Thi Nhu Huong, Le Phuong Thu, Nguyen Thi Mai Lan, Bui Nguyen Minh Thu, Nguyen Duc Dien
Due to its rising accumulation in the food chain and ongoing presence in ecosystems, metal contamination has piqued the curiosity of experts from all over the world. Environmentally hazardous heavy metal removal is being pursued utilizing a variety of techniques, such as ion exchange and precipitation, as well as chemical oxidation or reduction, electrochemistry, and filtration. However, these methods require high investment and operating costs, and generate toxic sludge. Spirulina platensis, a filamentous cyanobacteria species, has been reported as a potential bioadsorbent for the removal of some heavy metals from industrial wastewater. In this study, the bioadsorption of Zn2+ ions in an assumed aqueous solution by the dry biomass of S. platensis TH was investigated. The Zn2+ ion adsorption of biomaterials was evaluated under different conditions, including pH, contact time, temperature, and adsorbent mass. The optimal Zn2+ ion removal efficiency reached 90.32 ± 0.29% at Zn2+ ion concentration of 100 mg/L, pH 5.0, a temperature of 26oC, and a dry biomass dose of 1.5 g/L for 90 min. Langmuir and Freundlich's isothermal models were used to describe the adsorption isotherm of Zn2+ ions on S. platensis TH. Equilibrium data fitted well with the Langmuir model as well as the Freundlich model, with a maximum adsorption capacity of 34.56 mg Zn2+/g S. platensis TH under the reaction conditions of 1.5 g/L biomass dosage, the contact time of 90 min, pH 5.0, at 26oC. Research results have shown that S. platensis TH biomass is an easy, readily available, low-cost adsorbent and has a high bioadsorption capacity. Therefore, it can be treated as a bioadsorbent in the treatment of wastewater containing Zn2+ ions. This process is not only environmentally friendly but also versatile as an alternative to conventional heavy metal treatment methods.
{"title":"Evaluation on the Zn2+ ion adsorption capacity in water of Spirulina platensis biomaterial","authors":"Hoang Thi Quynh, Duong Thi Thuy, Doan Thi Oanh, Nguyen Thi Nhu Huong, Le Phuong Thu, Nguyen Thi Mai Lan, Bui Nguyen Minh Thu, Nguyen Duc Dien","doi":"10.15625/1811-4989/16604","DOIUrl":"https://doi.org/10.15625/1811-4989/16604","url":null,"abstract":"Due to its rising accumulation in the food chain and ongoing presence in ecosystems, metal contamination has piqued the curiosity of experts from all over the world. Environmentally hazardous heavy metal removal is being pursued utilizing a variety of techniques, such as ion exchange and precipitation, as well as chemical oxidation or reduction, electrochemistry, and filtration. However, these methods require high investment and operating costs, and generate toxic sludge. Spirulina platensis, a filamentous cyanobacteria species, has been reported as a potential bioadsorbent for the removal of some heavy metals from industrial wastewater. In this study, the bioadsorption of Zn2+ ions in an assumed aqueous solution by the dry biomass of S. platensis TH was investigated. The Zn2+ ion adsorption of biomaterials was evaluated under different conditions, including pH, contact time, temperature, and adsorbent mass. The optimal Zn2+ ion removal efficiency reached 90.32 ± 0.29% at Zn2+ ion concentration of 100 mg/L, pH 5.0, a temperature of 26oC, and a dry biomass dose of 1.5 g/L for 90 min. Langmuir and Freundlich's isothermal models were used to describe the adsorption isotherm of Zn2+ ions on S. platensis TH. Equilibrium data fitted well with the Langmuir model as well as the Freundlich model, with a maximum adsorption capacity of 34.56 mg Zn2+/g S. platensis TH under the reaction conditions of 1.5 g/L biomass dosage, the contact time of 90 min, pH 5.0, at 26oC. Research results have shown that S. platensis TH biomass is an easy, readily available, low-cost adsorbent and has a high bioadsorption capacity. Therefore, it can be treated as a bioadsorbent in the treatment of wastewater containing Zn2+ ions. This process is not only environmentally friendly but also versatile as an alternative to conventional heavy metal treatment methods.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77797283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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