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AquaSens: exploring the use of 16S rRNA next-generation sequencing to determine bacterial composition of various water matrices AquaSens:探索使用16S rRNA下一代测序来确定各种水基质的细菌组成
IF 1.5 4区 环境科学与生态学 Q4 WATER RESOURCES Pub Date : 2023-04-24 DOI: 10.17159/wsa/2023.v49.i2.3956
Nisreen Hoosain, Jeanne Korsman, Peter O Kimathi, Paidamoyo Kachambwa, Rembu Magoba, Shane L Murray
Access to clean water, one of the United Nation’s Sustainable Development Goals, is challenged by an increase in the presence of emerging microbial and other contaminants due to urbanization, among other factors. Traditionally, the presence of indicator microorganisms is determined using culturing methods. However, these classical methods cannot be used to determine the identities of ‘unknown’ bacteria and is limited to isolating the culturable state of microorganisms. Thus with culturing, the identities of many bacteria, particularly novel or non-culturable, may remain unknown. The use of a DNA-based method, 16S rRNA next-generation sequencing (NGS), can assist with determining the identities of bacterial populations in a water sample. The objective of this 16S rRNA NGS study was to investigate the bacterial community composition and diversity in a range of water sources. Water samples comprising of potable, surface, ground, marine, aquaculture, rain, wetland and swimming bath water matrices were subjected to 16S rRNA NGS using the Illumina 16S rRNA Metagenomics analysis pipeline. Operational taxonomic units were analysed and the identities of bacterial genera determined. In this study, genera of Acinetobacter, Mycobacterium, Pseudomonas, Legionella, Burkholderia, Yersinia, Staphylococcus and Vibrio were spread across the water matrices. Alpha (within sample) and beta (between samples) diversities for each bacterial community within the tested samples were also determined.
清洁水是联合国可持续发展目标之一,但由于城市化等因素,新出现的微生物和其他污染物的增加,对清洁水的获取提出了挑战。传统上,指示微生物的存在是通过培养方法来确定的。然而,这些经典方法不能用于确定“未知”细菌的身份,并且仅限于分离微生物的可培养状态。因此,通过培养,许多细菌的特性,特别是新的或不可培养的,可能仍然是未知的。使用基于dna的方法,16S rRNA下一代测序(NGS),可以帮助确定水样中细菌群体的身份。本16S rRNA NGS研究的目的是研究一系列水源中细菌群落的组成和多样性。使用Illumina 16S rRNA宏基因组学分析管道,对饮用水、地表水、地面水、海洋水、水产养殖水、雨水、湿地和游泳浴水基质进行16S rRNA NGS检测。分析了操作分类单位,确定了细菌属的身份。在本研究中,不动杆菌属、分枝杆菌属、假单胞菌属、军团菌属、伯克霍尔德氏菌属、耶尔森氏菌属、葡萄球菌属和弧菌属分布在水基质中。还确定了测试样品中每个细菌群落的α(样品内)和β(样品之间)多样性。
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引用次数: 1
On the use of late-time drawdown in interpreting aquifer pumping test 后期降差在含水层抽水试验解释中的应用
IF 1.5 4区 环境科学与生态学 Q4 WATER RESOURCES Pub Date : 2023-01-31 DOI: 10.17159/wsa/2023.v49.i1.3984
M Gomo
The review aims to provide a common understanding of the use of late-time drawdown to interpret aquifer pumping tests. The first part of the review provides an overview of the use of the late-time drawdown in literature to illustrate where and how the term is being used. A discussion on the practical implications of using the term and its significance is then presented. The review shows the use of the late-time drawdown in three main ways: the application of the Cooper and Jacob time-drawdown method, the description of the third segment of the unconfined aquifer drawdown-time curve, and when trying to estimate representative/effective transmissivity parameters in heterogeneous aquifers. Unlike the other two situations, the use of late-time data in typical unconfined aquifers is supported by the groundwater flow principles and hence has a meaningful application. The aspects highlighted in this review are important to improve the theoretical and practical knowledge required for analysing and interpreting aquifer pumping test data.
这篇综述的目的是为使用后期降差来解释含水层抽水试验提供一个共同的理解。回顾的第一部分概述了文献中使用的后期缩减,以说明该术语在何处以及如何使用。然后讨论了使用该术语的实际含义及其意义。回顾显示了三种主要的后期递减方法:Cooper和Jacob时间递减法的应用,无承压含水层递减时间曲线第三段的描述,以及在非均质含水层中尝试估计代表性/有效透射率参数。与其他两种情况不同,在典型的无承压含水层中使用后期数据得到地下水流动原理的支持,因此具有重要的应用价值。这篇综述强调的方面对于提高分析和解释含水层抽水试验数据所需的理论和实践知识是重要的。
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引用次数: 0
Adsorptive removal of BTEX compounds from wastewater using activated carbon derived from macadamia nut shells 用源自夏威夷坚果壳的活性炭吸附去除废水中的BTEX化合物
IF 1.5 4区 环境科学与生态学 Q4 WATER RESOURCES Pub Date : 2023-01-31 DOI: 10.17159/wsa/2023.v49.i1.3970
Kedibone Melaphi, Olawumi O Sadare, Geoffrey S Simate, Stephan Wagenaar, Kapil Moothi
In this study, adsorptive removal of benzene, toluene, ethylbenzene and xylenes (BTEX) from synthetic water using activated carbon adsorbent derived from macadamia nut shells was investigated. The surface functional groups of the synthesized adsorbents were assessed by Fourier transform infrared spectra. The specific surface area, pore size and pore volume at 77 K nitrogen adsorption, surface morphology, and the crystalline structure of the adsorbents were determined using Brunauer-Emmett-Teller, scanning electron microscopy and x-ray diffraction, respectively. Batch adsorption mode was used to evaluate the performance of the activated carbon.  The stock solutions of synthetic wastewater were prepared by dissolving 100 mg/L of each of the BTEX compound into distilled water in a 250 mL volumetric flask. Effect of initial concentration of BTEX compounds, contact time, and mass of adsorbent on the removal of BTEX compounds from the synthetic wastewater was investigated. The macadamia nut shell–derived activated carbon (MAC) proved to be an effective adsorbent for BTEX compounds, with a large surface area of 405.56 m2/g. The exposure time to reach equilibrium for maximum removal of BTEX was observed to be 20 min.  The adsorption capacity of the BTEX compounds by MAC followed the following adsorption order: benzene > toluene > ethylbenzene ˃ xylene.
研究了以澳洲坚果壳为原料制备的活性炭吸附剂对合成水中苯、甲苯、乙苯和二甲苯(BTEX)的吸附去除。利用傅里叶变换红外光谱对合成吸附剂的表面官能团进行了表征。采用brunauer - emmet - teller、扫描电镜和x射线衍射分别测定了吸附剂在77 K氮气吸附时的比表面积、孔径和孔体积、表面形貌和晶体结构。采用间歇吸附方式对活性炭的性能进行了评价。制备合成废水的原液,将每种BTEX化合物100 mg/L溶于250 mL容量瓶中的蒸馏水中。考察了BTEX化合物的初始浓度、接触时间和吸附剂质量对合成废水中BTEX化合物去除率的影响。夏威夷果壳活性炭(MAC)具有405.56 m2/g的表面积,是一种有效的BTEX类化合物吸附剂。观察到达到最大去除BTEX平衡的暴露时间为20 min。MAC对BTEX化合物的吸附量顺序为:苯>甲苯>乙苯_二甲苯。
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引用次数: 1
Moisture content distribution model for the soil wetting body under moistube irrigation 水分灌溉条件下土壤湿体含水率分布模型
IF 1.5 4区 环境科学与生态学 Q4 WATER RESOURCES Pub Date : 2023-01-31 DOI: 10.17159/wsa/2023.v49.i1.3979
Yanwei Fan, Weifan Yin, Zhiwei Yang, Yanxiang Wang, Liangjun Ma
This study investigated the moisture distribution characteristics of a soil wetting body under different influencing factors to inform the design and management of a moistube irrigation system. A mathematical model of soil moisture movement under moistube irrigation was established based on Hydrus-2D software. The suitability of the Hydrus-2D simulation model was verified by laboratory experiments. Numerical simulations were carried out with Hydrus-2D to investigate the influence of soil texture, initial moisture content, moistube specific discharge and irrigation time on the moisture distribution of a soil wetting body. The soil moisture content is highest at the moistube, and its value is related to the moistube-specific discharge and soil texture. The soil moisture content at any point in the wetting body decreased linearly with increasing distance from the wetting front to the moistube in the five set directions (vertical downward, 45° downward, horizontal, 45° upward and vertical upward). This trend is applicable to fine-textured and coarse-textured soil. An estimation model of soil moisture content including soil saturated hydraulic conductivity, initial soil moisture, the specific flow rate of the moistube and the maximum value of the wetting front distance in all directions is proposed. The model estimation is good (root mean square error = 0.008–0.018 cm3·cm−3, close to 0; Nash-Sutcliffe efficiency coefficient = 0.987, close to 1), and it can provide a practical tool for moistube irrigation design and agricultural water management.
研究了不同影响因素下土壤湿体的水分分布特征,为水分灌溉系统的设计和管理提供依据。基于Hydrus-2D软件,建立了滴灌条件下土壤水分运动的数学模型。通过室内实验验证了Hydrus-2D仿真模型的适用性。利用Hydrus-2D软件进行了数值模拟,研究了土壤质地、初始含水率、水比流量和灌溉时间对土壤湿体水分分布的影响。土壤含水率在湿管处最高,其数值与湿比流量和土壤质地有关。在垂直向下、45°向下、水平向上、45°向上和垂直向上5个设定方向上,润湿体各点土壤含水量随润湿锋到润湿管距离的增加呈线性下降。这种趋势适用于细质土和粗质土。提出了包括土壤饱和导水率、土壤初始含水量、湿管比流量和各方向湿润锋距最大值在内的土壤含水量估算模型。模型估计良好(均方根误差= 0.008-0.018 cm3·cm−3,接近于0;Nash-Sutcliffe效率系数= 0.987,接近于1),可为滴灌设计和农业用水管理提供实用工具。
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引用次数: 1
Using Landsat satellite imagery to monitor the spatial and temporal dynamics of aquatic weed extent in Lakes Chivero and Manyame, located in an urban catchment of Zimbabwe 利用陆地卫星图像监测津巴布韦城市集水区奇韦罗湖和Manyame湖水草范围的时空动态
IF 1.5 4区 环境科学与生态学 Q4 WATER RESOURCES Pub Date : 2023-01-31 DOI: 10.17159/wsa/2023.v49.i1.3950
Munyaradzi Davis Shekede, Takudzwa Gondo, Melisa Matavire Mavhenge, Aldridge Nyasha Mazhindu
This study quantified the spatial and temporal variation of aquatic weeds in two lakes in an urban catchment of Zimbabwe using the automatic water extraction index (AWEI) and normalised difference vegetation index (NDVI) derived from Landsat satellite data from 1986 to 2020. Extent of aquatic weeds estimated using AWEI in Lake Chivero increased from less than 1 km2 (4%) in 1986 to 7 km2 (27%) in 2020. NDVI-based aquatic weed estimation gave the least spatial extent in the first few years. Similarly, in Lake Manyame aquatic weeds occupied ~62 ha (<1% in 1986) before reaching a peak extent of 60 km2 (~70%) in 1995, based on AWEI estimates. NDVI-derived aquatic weed extent ranged from less than 2 km2 in 1997 to a maximum of 56.12 km2 in 1994. Although AWEI and NDVI estimated similar extents, NDVI had higher estimates than AWEI. A non-significant positive trend in aquatic weed extent was detected for Lake Manyame based on AWEI (Mann-Kendal tau = 0.139, s = 69, p = 0.27) and NDVI (Mann-Kendal tau = 0.129, s = 64, p = 0.307). In Lake Chivero, a non-significant negative trend was observed in aquatic weed extent based on NDVI (Mann-Kendal tau = −0.06, s = −30, p = 0.6382), while a positive trend was detected using AWEI (tau = 0.0036, s = 18, p = 0.7827). Results of the regression analysis indicate that phosphorus (R2 = 0.7957, p = 0.00122) and nitrogen (R2 = 0.8992, p = 0.0011) significantly explained variations in aquatic weed infestation in Lake Chivero. These results suggest that phosphorus and nitrogen enrichment are key drivers of aquatic weed proliferation in the two lakes. Thus, sustainable management of water resources in the catchment hinges on reducing the amount of nutrients released into the lakes from sewage treatment plants and croplands.
基于1986 - 2020年Landsat卫星数据,采用自动取水指数(AWEI)和归一化植被差异指数(NDVI)对津巴布韦城市集水区两个湖泊水草的时空变化进行了量化。利用awi估算的Chivero湖水草面积从1986年的不足1 km2(4%)增加到2020年的7 km2(27%)。基于ndvi的水草估算在前几年的空间范围最小。同样,根据AWEI的估计,在1995年达到60 km2(~70%)的峰值之前,Manyame湖的水草面积约为62 ha(1986年<1%)。ndvi衍生水草范围从1997年的不足2 km2到1994年的最大56.12 km2不等。尽管AWEI和NDVI估计的范围相似,但NDVI的估计高于AWEI。基于awi (Mann-Kendal tau = 0.139, s = 69, p = 0.27)和NDVI (Mann-Kendal tau = 0.129, s = 64, p = 0.307)的分析结果显示,湖水草面积呈非显著性正相关。基于NDVI的奇维罗湖水草面积呈非显著负向(Mann-Kendal tau = - 0.06, s = - 30, p = 0.6382),而基于awi的水草面积呈正向(tau = 0.0036, s = 18, p = 0.7827)。回归分析结果表明,磷(R2 = 0.7957, p = 0.00122)和氮(R2 = 0.8992, p = 0.0011)显著解释了奇韦罗湖水草侵染的变化。这些结果表明,磷和氮的富集是两个湖泊水草繁殖的关键驱动因素。因此,集水区水资源的可持续管理取决于减少从污水处理厂和农田释放到湖泊中的营养物质的数量。
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引用次数: 0
Application of biopolymer in turbidity removal and sludge settling behaviour of travertine-processing wastewater: Performance optimization using response surface methodology (RSM) 生物聚合物在石灰华废水除浊和污泥沉降中的应用:利用响应面法(RSM)进行性能优化
IF 1.5 4区 环境科学与生态学 Q4 WATER RESOURCES Pub Date : 2023-01-31 DOI: 10.17159/wsa/2023.v49.i1.3952
Ebru Taş, Emmanuel lkechukwu Ugwu, Eyüp Sabah, Zeyni Arsoy
A flocculation process was performed to treat travertine-processing effluents with a high concentration of suspended solids using an eco-friendly biopolymer. The experiments were conducted through a standard jar test procedure to optimize the process parameters for sludge volume index (SVI) and turbidity removal. The effects of mixing time, suspension pH, and polymer dosage on treatment efficiency were investigated using central composite design, a standard technique in response surface methodology. The constructed response model was tested using the analysis of variance (ANOVA). Using the Design-Expert tool, the coefficients of regression models were computed. The Fischer value (F-value) was used to evaluate the significance and validity of the predicted model, while the coefficient of determination (R2) was applied to estimate the model significance by comparing the predicted data with the measured data. The optimized parameters obtained were polymer dose of 276.20 mg/L, suspension pH of 8.60, and mixing time of 4.20 min. The optimal SVI and turbidity values obtained were 1.36 mL/g and 2.99 NTU, respectively. Additionally, R2 values for SVI and turbidity were determined as 0.9337 and 0.8654, respectively. Also, the difference between adjusted R2 values and predicted R2 was less than 0.2. Validation tests showed that the response surface methodology is an effective method for optimizing the flocculation mechanism.
采用生态友好型生物聚合物对含高浓度悬浮物的石灰华废水进行了絮凝处理。通过标准罐试验程序进行试验,以优化污泥体积指数(SVI)和浊度去除的工艺参数。采用响应面法中的标准方法——中心复合设计,考察了混合时间、悬浮液pH和聚合物用量对处理效率的影响。采用方差分析(ANOVA)对构建的反应模型进行检验。利用Design-Expert工具计算回归模型的系数。采用Fischer值(f值)评价预测模型的显著性和有效性,采用决定系数(R2)通过比较预测数据与实测数据来估计模型的显著性。优化参数为聚合物用量276.20 mg/L,悬浮液pH为8.60,搅拌时间为4.20 min,最佳SVI值为1.36 mL/g,浊度值为2.99 NTU。SVI和浊度的R2值分别为0.9337和0.8654。调整后的R2值与预测的R2值的差异小于0.2。验证试验表明,响应面法是优化絮凝机理的有效方法。
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引用次数: 0
Forward osmosis treatment of thermal evaporator brine stream 热蒸发器正渗透处理盐水流
IF 1.5 4区 环境科学与生态学 Q4 WATER RESOURCES Pub Date : 2023-01-31 DOI: 10.17159/wsa/2023.v49.i1.3982
EN Sitabule, C Buckley
Forward osmosis technology was evaluated for treating evaporator brine stream from a petrochemical industry at bench scale using ammonium bicarbonate as a draw solution. Calcium carbonate scaling that forms from the interaction between the calcium ions in the feed solution and carbonate ions from the draw solution (reverse salt diffusion) leads to the reduction in water flux and water recoveries achievable (feed TDS concentration of ~60 000 mg‧L−1, calcium ~545 mg‧L−1 and draw solution (ammonium bicarbonate) concentration of ~240 000 mg‧L−1). Fouling can be prevented by softening the feedwater before it is treated in forward osmosis. Without calcium and magnesium, permeate fluxes and water recoveries of up to 6 L‧m−2‧h−1 and 60%, respectively, could be achieved. It was also observed in this study that the concentration of the calcium ions in the feed does have an impact on the formation of the calcium carbonate scale, implying that some hardness can be tolerated in the feed to the forward osmosis process. It can, therefore, be concluded that without some hardness removal, ammonium bicarbonate draw solution is not suitable for treating concentrated brine streams (e.g., evaporator brine) that contain a high concentration of calcium ions. FO technology using ammonium bicarbonate as a draw solution can be considered an alternative technology to treat concentrated brine streams from inland industries, provided some pre-treatment to remove scaling precursors such as calcium is incorporated in the flow scheme.
以碳酸氢铵为萃取液,对正向渗透技术处理石化工业蒸发器卤水流进行了实验研究。进料溶液中的钙离子与抽提液中的碳酸盐离子相互作用(反向盐扩散)形成碳酸钙结垢,导致水通量和水回收率降低(进料TDS浓度~ 60000 mg·L−1,钙~545 mg·L−1,抽提液(碳酸氢铵)浓度~ 240000 mg·L−1)。在正向渗透处理给水之前,可以通过软化给水来防止结垢。在不添加钙和镁的情况下,渗透通量和水回收率分别可达6 L·m·2·h−1和60%。本研究还观察到,饲料中钙离子的浓度对碳酸钙垢的形成确实有影响,这意味着饲料在正向渗透过程中可以耐受一定的硬度。因此,可以得出结论,如果没有一定的硬度去除,碳酸氢铵提取液不适合处理含有高浓度钙离子的浓盐水流(例如蒸发器盐水)。使用碳酸氢铵作为抽提液的FO技术可以被认为是处理内陆工业浓盐水流的替代技术,前提是在流动方案中加入一些去除结垢前体(如钙)的预处理。
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引用次数: 0
Prevalence of free-living acanthamoeba and its associated bacteria in energy-efficient hot water systems in South Africa 自由生活棘阿米巴及其相关细菌在南非节能热水系统中的流行
IF 1.5 4区 环境科学与生态学 Q4 WATER RESOURCES Pub Date : 2023-01-31 DOI: 10.17159/wsa/2023.v49.i1.3947
SJ Moodley, P Muchesa, C Bartie, TG Barnard, R Clarke, A Masenge, SN Venter
As part of the Eskom rebate programme, energy-efficient hot water systems such as solar water heaters (low pressure), heat pumps and energy-efficient showerheads were rolled out to the public as a measure to conserve and save energy.  There has been a concern that these systems may not reach the required high temperatures, especially during winter, and, as a result of this, Acanthamoeba and its associated bacteria such as Legionella pneumophila, Pseudomonas aeruginosa and nontuberculous mycobacteria could flourish within these systems causing a potential health risk to consumers.  This study examined the relationship between Acanthamoeba and its associated bacteria at different temperature ranges. A total of 156 water (69) and biofilm samples (87) were collected from a solar water heater, heat pump, geyser and showerheads and examined for these organisms using amoebal enrichment and molecular techniques.   Amoeba could be cultivated from 45 (65.2%) of the water samples and 56 (64.4%) of the biofilm samples. The study confirmed the presence of Legionella pneumophila, Pseudomonas aeruginosa and nontuberculous mycobacteria in the hot water systems at both of the simulated winter (20°C to 30°C) and higher summer (40°C to 55°C) temperatures as well as the control system.  There was a significant positive correlation between the presence of Acanthamoeba and the presence of Pseudomonas. Based on this association it is suggested that Pseudomonas aeruginosa could be investigated as an indicator organism for the presence of Acanthamoeba and opportunistic pathogens.
作为Eskom回扣计划的一部分,节能热水系统,如太阳能热水器(低压)、热泵和节能淋浴喷头,作为节约和节约能源的措施向公众推出。人们一直担心这些系统可能达不到所需的高温,特别是在冬季,因此,棘阿米巴及其相关细菌,如嗜肺军团菌、铜绿假单胞菌和非结核分枝杆菌可能在这些系统中繁殖,对消费者造成潜在的健康风险。本研究考察了棘阿米巴及其伴生细菌在不同温度范围内的关系。从太阳能热水器、热泵、间歇泉和淋浴喷头中收集了156个水(69个)和生物膜样本(87个),并使用阿米巴原虫富集和分子技术检测了这些生物。水样45份(65.2%)、生物膜56份(64.4%)可培养出变形虫。该研究证实,在模拟冬季(20°C至30°C)和较高夏季(40°C至55°C)温度的热水系统以及控制系统中均存在嗜肺军团菌、铜绿假单胞菌和非结核分枝杆菌。棘阿米巴的存在与假单胞菌的存在呈显著正相关。基于这种关联,建议铜绿假单胞菌可以作为棘阿米巴和条件致病菌存在的指示生物进行研究。
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引用次数: 0
Use of remote sensing to determine rainwater harvesting sites for piped micro-irrigation schemes in Chimanimani District, Zimbabwe 利用遥感确定津巴布韦奇马尼马尼地区管道微型灌溉计划的雨水收集地点
IF 1.5 4区 环境科学与生态学 Q4 WATER RESOURCES Pub Date : 2023-01-31 DOI: 10.17159/wsa/2023.v49.i1.3943
Mike M Mushayi, Samuel Kusangaya, Never Mujere
The eastern highlands of Zimbabwe, particularly Chimanimani District, are endowed with natural water bodies such as springs, pools, wetlands, puddles and river systems, which are potential sources of water for irrigated farming. Despite this, water challenges continue to exist due to rainfall seasonality and lack of suitable water harvesting sites. This calls for solutions to harness water in long-lasting sources to support the piped micro-irrigation schemes. These schemes are pillars in agricultural interventions such as horticulture, livestock farming, fish farming and beekeeping. This study therefore, determined potential rainwater harvesting (RWH) sites in Chimanimani District using geospatial techniques. Water pixels from Landsat 8 images were extracted using the normalised difference moisture index (NDMI) and normalized difference vegetation index (NDVI). Potential RWH sites were classified into land-based zones, wetlands and natural water bodies. Findings show that land-based zones cover 27.53%, wetlands cover 24.65% and water bodies cover 6.11% of the district. The study also indicates that integrating geographic information systems with remote-sensing tools is a useful approach in identifying RWH sites. Thus, this study provided a spatially explicit approach and presents a suitability map for RWH in Chimanimani District.
津巴布韦东部高地,特别是奇马尼马尼地区,拥有天然水体,如泉水、水池、湿地、水坑和河流系统,这些都是灌溉农业的潜在水源。尽管如此,由于降雨季节性和缺乏合适的集水地点,水资源挑战仍然存在。这需要解决方案来利用长期水源的水来支持管道微灌计划。这些计划是园艺、畜牧业、养鱼和养蜂等农业干预措施的支柱。因此,本研究利用地理空间技术确定了Chimanimani地区潜在的雨水收集(RWH)地点。利用归一化差水分指数(NDMI)和归一化差植被指数(NDVI)提取Landsat 8影像中的水像元。潜在的RWH地点被划分为陆基区、湿地和自然水体。结果表明,陆基带占全区面积的27.53%,湿地占24.65%,水体占6.11%。该研究还表明,将地理信息系统与遥感工具结合起来是确定RWH场址的一种有用方法。因此,本研究提供了一种空间明确的方法,并提出了Chimanimani地区RWH的适宜性图。
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引用次数: 0
Cyclopia subternata growth, yield, proline and relative water content in response to water deficit stress 水亏缺胁迫对沙棘生长、产量、脯氨酸和相对含水量的响应
IF 1.5 4区 环境科学与生态学 Q4 WATER RESOURCES Pub Date : 2023-01-31 DOI: 10.17159/wsa/2023.v49.i1.3988
MS Mahlare, MN Lewu, FB Lewu, C Bester
Cyclopia, generally known as honeybush, and belonging to the Fabaceae family, originates from the Cape Floristic Region of the Eastern Cape and Western Cape provinces of South Africa. Currently, 6 honeybush species are commercially cultivated but, to date, there have been limited trials attempting to study their agronomic water demand. A pot trial was conducted where Cyclopia subternata plants were cultivated on different soil types (Stellenbosch granite, Stellenbosch shale and Stellenbosch clovelly) and subjected to three different water-deficit stress levels (well-watered, semi-stressed and stressed). Remarkably, irrigation treatments and soil types did not significantly affect the growth of the plants. However, the well-watered treatment consistently had higher yields compared to the other two treatments. The water-stressed (semi-stressed and stressed) treatments had lower relative water contents (RWC) with higher concentrations of proline, which signify water stress, compared to the control treatment. Higher proline and lower RWC contents found in this study are indications of water stress.
独角蜂,俗称蜜丛,属于豆科,原产于南非东开普省和西开普省的开普区。目前,有6种蜜丛被商业化栽培,但迄今为止,研究其农业用水需求的试验有限。采用盆栽试验的方法,在不同土壤类型(花岗岩、页岩和四叶草)上栽培Cyclopia subternata植株,并对其进行3种不同的水分亏缺胁迫水平(丰水、半干旱和干旱)。灌溉处理和土壤类型对植株生长无显著影响。然而,与其他两种处理相比,水分充足的处理始终具有更高的产量。与对照处理相比,水分胁迫(半胁迫和胁迫)处理的相对含水量(RWC)较低,脯氨酸浓度较高,表明水分胁迫。较高的脯氨酸和较低的RWC含量是水分胁迫的标志。
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引用次数: 0
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