首页 > 最新文献

Veterinary Research Communications最新文献

英文 中文
Limosilactobacillus reuteri B1/1 modulated the intestinal immune response in preventing Salmonella Enteritidis PT4 infection in a chicken ileal explant model. 在鸡回肠外植体模型中,Limosilactobacillus reuteri B1/1 可调节肠道免疫反应,预防肠炎沙门氏菌 PT4 感染。
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-11-23 DOI: 10.1007/s11259-024-10609-4
Viera Karaffová, Zuzana Kiššová, Csilla Tóthová, Patrik Tráj, Máté Mackei, Gábor Mátis

In this study, we observed the effect of the newly isolated probiotic strain Limosilactobacillus reuteri B1/1 on the relative gene expression of selected cytokines (interleukin-15, transforming growth factor-β4), tight junction proteins (E-cadherin, occludin), biomarker active intestinal stem cells - LGR5 (leucine-rich repeat containing G protein-coupled receptor), markers of mucosal intestinal immunity (mucin-2, immunoglobulin A), as well as the creation of a new biomarker of inflammation in the intestine - calprotectin on an ex vivo model of chicken ileal explant in the prevention of Salmonella Enteritidis PT4 infection. The ability of L. reuteri B1/1 to effectively modulate the mucosal immune response under pretreatment conditions in S. Enteritidis PT4 infection in a chicken ileal explant model was confirmed. In addition, our obtained results point to the fact that the new chicken ileum explant model could be a suitable model to investigate or test the influence of natural substances such as probiotic bacteria in the interaction with the intestine as well as pathogenic microorganisms. In addition, the results of our study may contribute to a deeper understanding of the action of newly isolated probiotic bacteria at the intestinal level using ex vivo models such as chicken ileum explant, which are able to mimic in vivo conditions sufficiently.

在这项研究中,我们观察了新分离的益生菌株Limosilactobacillus reuteri B1/1对某些细胞因子(白细胞介素-15、转化生长因子-β4)、紧密连接蛋白(E-cadherin、occludin)、活跃的肠干细胞生物标志物--LGR5(富含亮氨酸重复的G蛋白偶联受体)的相对基因表达的影响、肠粘膜免疫标志物(粘蛋白-2、免疫球蛋白 A),以及在预防肠炎沙门氏菌 PT4 感染的鸡回肠外植体体外模型上创建了一种新的肠道炎症生物标志物--钙保护蛋白。在鸡回肠外植体模型中,证实了在肠炎沙门氏菌 PT4 感染的预处理条件下,L. reuteri B1/1 能够有效调节粘膜免疫反应。此外,我们的研究结果表明,新型鸡回肠外植体模型可作为研究或测试益生菌等天然物质与肠道及病原微生物相互作用影响的合适模型。此外,我们的研究结果可能有助于利用鸡回肠外植体等体内外模型,更深入地了解新分离的益生菌在肠道水平的作用,因为这种模型能够充分模拟体内条件。
{"title":"Limosilactobacillus reuteri B1/1 modulated the intestinal immune response in preventing Salmonella Enteritidis PT4 infection in a chicken ileal explant model.","authors":"Viera Karaffová, Zuzana Kiššová, Csilla Tóthová, Patrik Tráj, Máté Mackei, Gábor Mátis","doi":"10.1007/s11259-024-10609-4","DOIUrl":"https://doi.org/10.1007/s11259-024-10609-4","url":null,"abstract":"<p><p>In this study, we observed the effect of the newly isolated probiotic strain Limosilactobacillus reuteri B1/1 on the relative gene expression of selected cytokines (interleukin-15, transforming growth factor-β4), tight junction proteins (E-cadherin, occludin), biomarker active intestinal stem cells - LGR5 (leucine-rich repeat containing G protein-coupled receptor), markers of mucosal intestinal immunity (mucin-2, immunoglobulin A), as well as the creation of a new biomarker of inflammation in the intestine - calprotectin on an ex vivo model of chicken ileal explant in the prevention of Salmonella Enteritidis PT4 infection. The ability of L. reuteri B1/1 to effectively modulate the mucosal immune response under pretreatment conditions in S. Enteritidis PT4 infection in a chicken ileal explant model was confirmed. In addition, our obtained results point to the fact that the new chicken ileum explant model could be a suitable model to investigate or test the influence of natural substances such as probiotic bacteria in the interaction with the intestine as well as pathogenic microorganisms. In addition, the results of our study may contribute to a deeper understanding of the action of newly isolated probiotic bacteria at the intestinal level using ex vivo models such as chicken ileum explant, which are able to mimic in vivo conditions sufficiently.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"32"},"PeriodicalIF":1.8,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Treatment of mycotic rhinitis caused by aspergillus fumigatus in a quarter horse mare using topical clotrimazole and oral potassium iodide. 使用外用克霉唑和口服碘化钾治疗一匹四分马母马由曲霉菌引起的霉菌性鼻炎。
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-11-22 DOI: 10.1007/s11259-024-10570-2
Juliana de Moura Alonso, Marcos Jun Watanabe, Sandra de Moraes Gimenes Bosco, Emanuel Vitor Pereira Apolonio, Artur Bibiano de Vasconcelos, Ana Carolina do Prado, Ana Liz Garcia Alves, Celso Antonio Rodrigues, Carlos Alberto Hussni

Fungal rhinitis in horses is rare, although severe, due to the progressive nasal obstruction. Aspergillus is one of the fungi most often involved in these cases. A 7-year-old Quarter Horse mare weighing 325 kg was admitted with a history of recurrent nasal discharge and bleeding, foul smell, reluctance to exercise, and abortion. Laboratory and imaging examinations revealed eosinophilia, scarring and erosion areas in the nasal mucosa, purulent and bloody secretions, and remarkable yellowish plaques. Histopathological examination revealed fungal rhinitis and bacterial culture revealed the presence of β-hemolytic Streptococcus and Staphylococcus sp. Fungal culture and molecular diagnosis confirmed Aspergillus fumigatus as the etiological agent. A tracheostomy was necessary due to the patient's dyspnea. For the treatment, topical application of clotrimazole ointment was carried out in the rostral portion of the nasal cavity once a day and in the caudal portion of the nasal cavity, endoscopic guided every 48 h. In the fifth endoscopy-guided application, a significant improvement was noticed; however, the animal exhibited a hypersensitivity reaction, and the treatment was discontinued. Thus, potassium iodide therapy was initiated (67 mg/kg, PO, SID, 14 days). The patient demonstrated favorable clinical improvement, achieved resolution of the fungal infection, and was discharged after 25 days of hospitalization. Topical clotrimazole demonstrated a probable efficacy as a standalone treatment, although its use had to be prematurely discontinued due to the emergence of a hypersensitivity reaction. The combination of clotrimazole and potassium iodide effectively treated fungal rhinitis in a shorter time than previously reported. This report provides useful guidance for other cases of fungal rhinitis, although further studies are needed.

马的真菌性鼻炎虽然严重,但却很少见,因为它会造成进行性鼻塞。曲霉菌是此类病例中最常见的真菌之一。一匹 7 岁、体重 325 千克的季马母马因反复流鼻涕和出血、恶臭、不愿运动和流产而入院。实验室和影像学检查发现该马鼻黏膜有嗜酸性粒细胞增多、瘢痕和糜烂、脓性和血性分泌物以及明显的淡黄色斑块。组织病理学检查显示为真菌性鼻炎,细菌培养显示存在β-溶血性链球菌和葡萄球菌。由于患者呼吸困难,必须进行气管切开术。治疗时,每天一次在鼻腔喙部局部涂抹克霉唑软膏,每 48 小时一次在鼻腔尾部局部涂抹克霉唑软膏,并在内窥镜引导下进行。因此,开始了碘化钾治疗(67 毫克/千克,PO,SID,14 天)。患者的临床症状明显好转,真菌感染得到缓解,住院 25 天后出院。外用克霉唑作为一种单独的治疗方法,虽然显示出了可能的疗效,但由于出现了超敏反应,不得不提前停止使用。与之前的报道相比,克霉唑和碘化钾联合使用能在更短的时间内有效治疗真菌性鼻炎。本报告为其他真菌性鼻炎病例提供了有用的指导,尽管还需要进一步研究。
{"title":"Treatment of mycotic rhinitis caused by aspergillus fumigatus in a quarter horse mare using topical clotrimazole and oral potassium iodide.","authors":"Juliana de Moura Alonso, Marcos Jun Watanabe, Sandra de Moraes Gimenes Bosco, Emanuel Vitor Pereira Apolonio, Artur Bibiano de Vasconcelos, Ana Carolina do Prado, Ana Liz Garcia Alves, Celso Antonio Rodrigues, Carlos Alberto Hussni","doi":"10.1007/s11259-024-10570-2","DOIUrl":"10.1007/s11259-024-10570-2","url":null,"abstract":"<p><p>Fungal rhinitis in horses is rare, although severe, due to the progressive nasal obstruction. Aspergillus is one of the fungi most often involved in these cases. A 7-year-old Quarter Horse mare weighing 325 kg was admitted with a history of recurrent nasal discharge and bleeding, foul smell, reluctance to exercise, and abortion. Laboratory and imaging examinations revealed eosinophilia, scarring and erosion areas in the nasal mucosa, purulent and bloody secretions, and remarkable yellowish plaques. Histopathological examination revealed fungal rhinitis and bacterial culture revealed the presence of β-hemolytic Streptococcus and Staphylococcus sp. Fungal culture and molecular diagnosis confirmed Aspergillus fumigatus as the etiological agent. A tracheostomy was necessary due to the patient's dyspnea. For the treatment, topical application of clotrimazole ointment was carried out in the rostral portion of the nasal cavity once a day and in the caudal portion of the nasal cavity, endoscopic guided every 48 h. In the fifth endoscopy-guided application, a significant improvement was noticed; however, the animal exhibited a hypersensitivity reaction, and the treatment was discontinued. Thus, potassium iodide therapy was initiated (67 mg/kg, PO, SID, 14 days). The patient demonstrated favorable clinical improvement, achieved resolution of the fungal infection, and was discharged after 25 days of hospitalization. Topical clotrimazole demonstrated a probable efficacy as a standalone treatment, although its use had to be prematurely discontinued due to the emergence of a hypersensitivity reaction. The combination of clotrimazole and potassium iodide effectively treated fungal rhinitis in a shorter time than previously reported. This report provides useful guidance for other cases of fungal rhinitis, although further studies are needed.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"28"},"PeriodicalIF":1.8,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
CT findings and anatomical aspects of a Labrador retriver humerus characterized by osteophytosis of the sulcus intertubercularis and obliteration of the supratrochlear foramen. 拉布拉多猎犬肱骨的 CT 结果和解剖学特征:肱骨间沟骨质增生,肱骨髁上孔闭塞。
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-11-22 DOI: 10.1007/s11259-024-10583-x
Sedef Selviler-Sizer, Semih Kurt, Burcu Onuk, Murat Kabak

Degenerative changes in the skeletal system of dogs may occur as a consequence of aging, trauma, infection, or inflammation. The object of this study consisted of an 8-year-old male Labrador dog that was brought to a private veterinary clinic after a road traffic accident and died despite all interventions. For a different study planned in our department, CT images were taken of the Labrador retriever's humerus and osteophytes to encounter incidentally were investigated for found in these images. After the CT scan, the dissected humerus bones were boiled to remove soft tissues. Subsequently, the bones were underwent with a 10% hydrogen peroxide solution for bleaching. Osteophytes in the proximal part of the humerus extended from the tuberculum majus and tuberculum minus towards the cranial direction, forming a complete ring-like structure at the level of the sulcus intertubercularis in the left humerus. Although not fully ring-shaped, similar osteophytes were found in the right humerus. In addition, it was observed that the foramen supratrochleare seen in the left humerus in the CT images was covered with a thin membrane during dissection. The presence of this membrane suggested that it might be due to the joint not being able to reach sufficient extension due to osteophytes forming in the bone. Additionally, it was concluded that CT may not recognize thin, non-mineralized septations, such as the membrane obliterating the supratrochlear foramen, as observed during necropsy.

狗骨骼系统的退行性变化可能是衰老、创伤、感染或炎症的结果。本研究的对象是一只 8 岁的雄性拉布拉多猎犬,它在一次道路交通事故后被送到一家私人兽医诊所,在接受了所有干预措施后死亡。在本部门计划进行的另一项研究中,我们为拉布拉多猎犬的肱骨拍摄了 CT 图像,并对这些图像中偶然发现的骨质增生进行了调查。CT 扫描后,将解剖的肱骨煮沸以去除软组织。随后,用 10% 过氧化氢溶液对骨骼进行漂白。肱骨近端骨质增生从大结节和小结节向头颅方向延伸,在左侧肱骨的结节间沟处形成一个完整的环状结构。右肱骨中也发现了类似的骨质增生,但并非完全环形。此外,还观察到在 CT 图像中看到的左肱骨肘上孔在解剖时被一层薄膜覆盖。这层薄膜的存在表明,这可能是由于骨质增生导致关节无法充分伸展所致。此外,研究还得出结论,CT 可能无法识别薄的非矿化隔膜,如尸检时观察到的阻塞椎上孔的薄膜。
{"title":"CT findings and anatomical aspects of a Labrador retriver humerus characterized by osteophytosis of the sulcus intertubercularis and obliteration of the supratrochlear foramen.","authors":"Sedef Selviler-Sizer, Semih Kurt, Burcu Onuk, Murat Kabak","doi":"10.1007/s11259-024-10583-x","DOIUrl":"10.1007/s11259-024-10583-x","url":null,"abstract":"<p><p>Degenerative changes in the skeletal system of dogs may occur as a consequence of aging, trauma, infection, or inflammation. The object of this study consisted of an 8-year-old male Labrador dog that was brought to a private veterinary clinic after a road traffic accident and died despite all interventions. For a different study planned in our department, CT images were taken of the Labrador retriever's humerus and osteophytes to encounter incidentally were investigated for found in these images. After the CT scan, the dissected humerus bones were boiled to remove soft tissues. Subsequently, the bones were underwent with a 10% hydrogen peroxide solution for bleaching. Osteophytes in the proximal part of the humerus extended from the tuberculum majus and tuberculum minus towards the cranial direction, forming a complete ring-like structure at the level of the sulcus intertubercularis in the left humerus. Although not fully ring-shaped, similar osteophytes were found in the right humerus. In addition, it was observed that the foramen supratrochleare seen in the left humerus in the CT images was covered with a thin membrane during dissection. The presence of this membrane suggested that it might be due to the joint not being able to reach sufficient extension due to osteophytes forming in the bone. Additionally, it was concluded that CT may not recognize thin, non-mineralized septations, such as the membrane obliterating the supratrochlear foramen, as observed during necropsy.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"27"},"PeriodicalIF":1.8,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Etiology and antimicrobial resistance of subclinical mastitis pathogens Staphylococcus aureus, Streptococcus spp. and Enterococcus spp. in sheep milk. 绵羊奶中亚临床乳腺炎病原体金黄色葡萄球菌、链球菌属和肠球菌属的病因和抗菌药耐药性。
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-11-22 DOI: 10.1007/s11259-024-10579-7
Ionica Iancu, Violeta Igna, Sebastian Alexandru Popa, Kálmán Imre, Corina Pascu, Luminița Costinar, Janos Degi, Alexandru Gligor, Vlad Iorgoni, Corina Badea, Ileana Nichita, Viorel Herman

The present study investigates the prevalence and etiology of subclinical mastitis in Țurcana sheep flocks located in south-western Romania. Milchtest and California Mastitis Test (CMT), were used for the detection of subclinical mastitis. A number of 360 milk samples across three lactation phases were analyzed. Subclinical mastitis was identified in 146 (40.6%) of sheep milk samples. Only milk samples (n = 146) collected from sheep diagnosed with subclinical mastitis were considered for microbiological analyses. Thus, Staphylococcus aureus (129/146; 88.4%) was identified as the predominant pathogen followed by Streptococcus spp. (14/146; 9.6%) Enterococcus spp. (7/146; 4.8%), Pseudomonas aeruginosa (6/146; 4.1%) and Klebsiella pneumoniae (5/146; 3.4%). Antimicrobial susceptibility of isolated strains of Staphylococcus aureus, Streptococcus spp. and Enterococcus spp. was also tested. The results showed varying resistance patterns depending on the farm and microorganism. In heard A the highest resistance of Staphylococcus aureus was identified to polymyxin B (79.7%) followed by novobiocin (73.4%) and tetracycline (45.3%); Streptococcus spp. to streptomycin (100%), novobiocin (100%), tetracycline (100%) and Enterococcus spp. to tylosin (100%), streptomycin (75.0%) and tetracycline (75.0%). In herd B, Staphylococcus aureus showed high resistance rates to polymyxin B (86.3%), novobiocin (74.5%) and cloxacillin (39.2%); Streptococcus spp. strains to streptomycin (100%), novobiocin (100%), tetracycline (100%) and Enterococcus spp. to gentamicin (100%), streptomycin (66.7%) and erythromycin (66.7%). The obtained preliminary results indicate a potential risk that future treatments of sheep mastitis may not be effective, and demonstrated that raw unpasteurized milk can harbor antimicrobial resistance pathogens posing a threat to public health. However, further investigations involving molecular research on resistance genes are required to draw comprehensive conclusions.

本研究调查了罗马尼亚西南部Țurcana 羊群亚临床乳腺炎的发病率和病因。亚临床乳腺炎的检测采用了 Milchtest 和加利福尼亚乳腺炎测试(CMT)。对三个哺乳期的 360 份牛奶样本进行了分析。在 146 份(40.6%)羊奶样本中发现了亚临床乳腺炎。只有从确诊患有亚临床乳腺炎的绵羊身上采集的牛奶样本(n = 146)才被考虑用于微生物分析。因此,金黄色葡萄球菌(129/146;88.4%)被确定为主要病原体,其次是链球菌属(14/146;9.6%)、肠球菌属(7/146;4.8%)、铜绿假单胞菌(6/146;4.1%)和肺炎克雷伯菌(5/146;3.4%)。此外,还检测了金黄色葡萄球菌、链球菌属和肠球菌属分离菌株的抗菌药敏感性。结果显示,不同农场和微生物的耐药性模式各不相同。在 A 群中,金黄色葡萄球菌对多粘菌素 B 的耐药性最高(79.7%),其次是新生物素(73.4%)和四环素(45.3%);链球菌对链霉素(100%)、新生物素(100%)和四环素(100%)产生耐药性;肠球菌对泰乐菌素(100%)、链霉素(75.0%)和四环素(75.0%)产生耐药性。在 B 组中,金黄色葡萄球菌对多粘菌素 B(86.3%)、新生物素(74.5%)和氯霉素(39.2%)的耐药率较高;链球菌对链霉素(100%)、新生物素(100%)和四环素(100%)的耐药率较高;肠球菌对庆大霉素(100%)、链霉素(66.7%)和红霉素(66.7%)的耐药率较高。获得的初步结果表明,未来治疗羊乳腺炎的方法可能存在无效的潜在风险,并表明未经巴氏杀菌的生牛奶中可能含有抗菌素耐药性病原体,对公共卫生构成威胁。不过,要得出全面的结论,还需要进一步开展抗药性基因的分子研究。
{"title":"Etiology and antimicrobial resistance of subclinical mastitis pathogens Staphylococcus aureus, Streptococcus spp. and Enterococcus spp. in sheep milk.","authors":"Ionica Iancu, Violeta Igna, Sebastian Alexandru Popa, Kálmán Imre, Corina Pascu, Luminița Costinar, Janos Degi, Alexandru Gligor, Vlad Iorgoni, Corina Badea, Ileana Nichita, Viorel Herman","doi":"10.1007/s11259-024-10579-7","DOIUrl":"10.1007/s11259-024-10579-7","url":null,"abstract":"<p><p>The present study investigates the prevalence and etiology of subclinical mastitis in Țurcana sheep flocks located in south-western Romania. Milchtest and California Mastitis Test (CMT), were used for the detection of subclinical mastitis. A number of 360 milk samples across three lactation phases were analyzed. Subclinical mastitis was identified in 146 (40.6%) of sheep milk samples. Only milk samples (n = 146) collected from sheep diagnosed with subclinical mastitis were considered for microbiological analyses. Thus, Staphylococcus aureus (129/146; 88.4%) was identified as the predominant pathogen followed by Streptococcus spp. (14/146; 9.6%) Enterococcus spp. (7/146; 4.8%), Pseudomonas aeruginosa (6/146; 4.1%) and Klebsiella pneumoniae (5/146; 3.4%). Antimicrobial susceptibility of isolated strains of Staphylococcus aureus, Streptococcus spp. and Enterococcus spp. was also tested. The results showed varying resistance patterns depending on the farm and microorganism. In heard A the highest resistance of Staphylococcus aureus was identified to polymyxin B (79.7%) followed by novobiocin (73.4%) and tetracycline (45.3%); Streptococcus spp. to streptomycin (100%), novobiocin (100%), tetracycline (100%) and Enterococcus spp. to tylosin (100%), streptomycin (75.0%) and tetracycline (75.0%). In herd B, Staphylococcus aureus showed high resistance rates to polymyxin B (86.3%), novobiocin (74.5%) and cloxacillin (39.2%); Streptococcus spp. strains to streptomycin (100%), novobiocin (100%), tetracycline (100%) and Enterococcus spp. to gentamicin (100%), streptomycin (66.7%) and erythromycin (66.7%). The obtained preliminary results indicate a potential risk that future treatments of sheep mastitis may not be effective, and demonstrated that raw unpasteurized milk can harbor antimicrobial resistance pathogens posing a threat to public health. However, further investigations involving molecular research on resistance genes are required to draw comprehensive conclusions.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"30"},"PeriodicalIF":1.8,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The interrelationship between macrominerals and heat stress in ruminants: current perspectives and future directions - a review. 反刍动物体内大分子矿物质与热应激之间的相互关系:当前视角与未来方向--综述。
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-11-22 DOI: 10.1007/s11259-024-10597-5
Emad M Samara, Mohammed A Al-Badwi, Khalid A Abdoun, Mutassim M Abdelrahman, Aly B Okab, Majdi A Bahadi, Ahmed A Al-Haidary

Macromineral imbalances in ruminants, particularly in tropical and subtropical regions, pose a significant challenge to production sustainability and profitability. Heat stress exacerbates these imbalances, negatively impacting physiological functions and productivity. This review examines the effects of heat stress on macromineral levels in ruminants and the need for supplementation under such conditions. Heat stress lowers key macrominerals (Na+, K+, Cl-, Ca + 2, Mg + 2, inorganic P) and disrupts acid-base balance due to thermoregulatory responses and reduced feed intake. Supplementing macrominerals to the diet to achieve higher dietary cation-anion difference helps mitigate heat-related morbidity and maintains ruminant health and productivity. A more practical approach, such as sustained-release macromineral boluses in the rumen, is proposed to provide more consistent benefits. Further researches are warranted to optimize supplementation strategies and fully understand macromineral nutrition for heat-stressed ruminants.

反刍动物体内的大矿物质失衡,尤其是在热带和亚热带地区,对生产的可持续性和盈利能力构成了重大挑战。热应激会加剧这些失衡,对生理功能和生产率产生负面影响。本综述探讨了热应激对反刍动物体内大矿物质水平的影响,以及在这种情况下补充大矿物质的必要性。热应激会降低主要大矿物质(Na+、K+、Cl-、Ca + 2、Mg + 2、无机磷)的含量,并因体温调节反应和采食量减少而破坏酸碱平衡。在日粮中补充大矿物质以达到较高的日粮阳离子-阴离子差,有助于减轻与热有关的发病率,并保持反刍动物的健康和生产力。建议采用更实用的方法,如在瘤胃中持续释放大矿物质栓剂,以提供更稳定的益处。为了优化补充策略并充分了解热应激反刍动物的大矿物质营养,有必要开展进一步的研究。
{"title":"The interrelationship between macrominerals and heat stress in ruminants: current perspectives and future directions - a review.","authors":"Emad M Samara, Mohammed A Al-Badwi, Khalid A Abdoun, Mutassim M Abdelrahman, Aly B Okab, Majdi A Bahadi, Ahmed A Al-Haidary","doi":"10.1007/s11259-024-10597-5","DOIUrl":"10.1007/s11259-024-10597-5","url":null,"abstract":"<p><p>Macromineral imbalances in ruminants, particularly in tropical and subtropical regions, pose a significant challenge to production sustainability and profitability. Heat stress exacerbates these imbalances, negatively impacting physiological functions and productivity. This review examines the effects of heat stress on macromineral levels in ruminants and the need for supplementation under such conditions. Heat stress lowers key macrominerals (Na+, K+, Cl-, Ca + 2, Mg + 2, inorganic P) and disrupts acid-base balance due to thermoregulatory responses and reduced feed intake. Supplementing macrominerals to the diet to achieve higher dietary cation-anion difference helps mitigate heat-related morbidity and maintains ruminant health and productivity. A more practical approach, such as sustained-release macromineral boluses in the rumen, is proposed to provide more consistent benefits. Further researches are warranted to optimize supplementation strategies and fully understand macromineral nutrition for heat-stressed ruminants.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"29"},"PeriodicalIF":1.8,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Efficacy of Lactobacillus acidophilus and yeast cell wall-derived supplements on immunity responses, growth performance, and disease resistance in Cyprinus carpio juveniles. 嗜酸乳杆菌和酵母细胞壁补充剂对鲤鱼幼鱼免疫反应、生长性能和抗病能力的影响
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-11-21 DOI: 10.1007/s11259-024-10567-x
Hamed Ghafarifarsani, Saman Ahani, Maryam Aftabgard, Sara Ahani, Morteza Yousefi

This study investigated the effects of probiotic Lactobacillus acidophilus (PTCC 1643) (LA) and a commercial yeast cell wall prebiotic (Immunogen®) (IM) on immunoantioxidant, growth, and digestive status, and resistance to Aeromonas hydrophila in Cyprinus carpio juveniles. The fish were fed in five treatments including control (T1), LA1.5 (T2): 1.5 (× 10^8 CFU/g of LA)/kg diet, LA3 (T3): 3 (× 10^8 CFU/g of LA)/kg diet, IM1 (T4): 1 g of IM/kg diet, and IM2 (T5): 2 g of IM/ kg diet. After 60 days, the function of growth and intestinal protease and amylase showed a significant increase in IM treatments. Serum levels of total protein and superoxide dismutase in LA3 treatment, as well as albumin, catalase, and glutathione peroxidase in LA3 and IM2 treatments were significantly increased. Serum level of malondialdehyde in LA and IM treatments, as well as alkaline phosphatase (ALP) in LA treatments, alanine aminotransferase in LA and IM2 treatments, and lactate dehydrogenase in LA3 treatment was significantly decreased. Serum levels of all the immune parameters in LA3 treatment, as well as alternative complement pathway hemolytic and nitroblue tetrazolium in IM2 treatment were significantly improved. Mucosal level of protease in LA3 and IM2 treatments, total immunoglobulin, and lysozyme in LA and IM2 treatments, as well as ALP and peroxidase in LA3 treatment was significantly increased. Cumulative mortality rate in LA and IM treatments was significantly decreased compared to control after the 14-day challenge with A. hydrophila. It is recommended to include IM2 in the diet of C. carpio juveniles with regard to its beneficial effects on growth and immunity status.

本研究调查了益生菌嗜酸乳杆菌(PTCC 1643)(LA)和商业酵母细胞壁益生菌(Immunogen®)(IM)对鲤鱼幼鱼免疫抗氧化、生长和消化状态以及对嗜水气单胞菌抵抗力的影响。对鲤鱼幼鱼进行了五种处理,包括对照组(T1)、LA1.5 组(T2)、LA1.5 组(T3)和 LA1.5 组(T4):1.5 (× 10^8 CFU/g LA)/千克日粮、LA3 (T3):3(× 10^8 CFU/g LA)/kg 日粮,IM1(T4):1 克 IM/千克日粮,以及 IM2(T5):2克IM/千克日粮。60 天后,肠蛋白酶和淀粉酶的生长和肠道功能在 IM 处理中显著增加。LA3处理中的血清总蛋白和超氧化物歧化酶水平,以及LA3和IM2处理中的白蛋白、过氧化氢酶和谷胱甘肽过氧化物酶水平均显著增加。LA和IM处理中的血清丙二醛水平、LA处理中的碱性磷酸酶(ALP)、LA和IM2处理中的丙氨酸氨基转移酶以及LA3处理中的乳酸脱氢酶均明显下降。LA3治疗中所有免疫指标的血清水平,以及IM2治疗中替代补体途径溶血和硝基蓝四氮唑的血清水平均有明显改善。LA3和IM2治疗中的粘膜蛋白酶水平、LA和IM2治疗中的总免疫球蛋白和溶菌酶水平,以及LA3治疗中的ALP和过氧化物酶水平均明显提高。与对照组相比,LA 和 IM 处理的累积死亡率在 14 天的嗜水蝇挑战后明显降低。建议在鲤鱼幼鱼的日粮中添加 IM2,因为它对生长和免疫状态有益。
{"title":"Efficacy of Lactobacillus acidophilus and yeast cell wall-derived supplements on immunity responses, growth performance, and disease resistance in Cyprinus carpio juveniles.","authors":"Hamed Ghafarifarsani, Saman Ahani, Maryam Aftabgard, Sara Ahani, Morteza Yousefi","doi":"10.1007/s11259-024-10567-x","DOIUrl":"https://doi.org/10.1007/s11259-024-10567-x","url":null,"abstract":"<p><p>This study investigated the effects of probiotic Lactobacillus acidophilus (PTCC 1643) (LA) and a commercial yeast cell wall prebiotic (Immunogen<sup>®</sup>) (IM) on immunoantioxidant, growth, and digestive status, and resistance to Aeromonas hydrophila in Cyprinus carpio juveniles. The fish were fed in five treatments including control (T1), LA1.5 (T2): 1.5 (× 10^8 CFU/g of LA)/kg diet, LA3 (T3): 3 (× 10^8 CFU/g of LA)/kg diet, IM1 (T4): 1 g of IM/kg diet, and IM2 (T5): 2 g of IM/ kg diet. After 60 days, the function of growth and intestinal protease and amylase showed a significant increase in IM treatments. Serum levels of total protein and superoxide dismutase in LA3 treatment, as well as albumin, catalase, and glutathione peroxidase in LA3 and IM2 treatments were significantly increased. Serum level of malondialdehyde in LA and IM treatments, as well as alkaline phosphatase (ALP) in LA treatments, alanine aminotransferase in LA and IM2 treatments, and lactate dehydrogenase in LA3 treatment was significantly decreased. Serum levels of all the immune parameters in LA3 treatment, as well as alternative complement pathway hemolytic and nitroblue tetrazolium in IM2 treatment were significantly improved. Mucosal level of protease in LA3 and IM2 treatments, total immunoglobulin, and lysozyme in LA and IM2 treatments, as well as ALP and peroxidase in LA3 treatment was significantly increased. Cumulative mortality rate in LA and IM treatments was significantly decreased compared to control after the 14-day challenge with A. hydrophila. It is recommended to include IM2 in the diet of C. carpio juveniles with regard to its beneficial effects on growth and immunity status.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"23"},"PeriodicalIF":1.8,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microbiota modulation by the inclusion of Tenebrio molitor larvae as alternative to fermented soy protein concentrate in growing pigs diet. 在生长猪日粮中添加褐飞虱幼虫替代发酵大豆浓缩蛋白调节微生物群。
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-11-21 DOI: 10.1007/s11259-024-10588-6
Irene Ferri, Matteo Dell'Anno, Alessandro Quiese, Bianca Castiglioni, Paola Cremonesi, Filippo Biscarini, Benedetta Canala, Matteo Santoru, Alessia Colombini, Giancarlo Ruffo, Antonella Baldi, Luciana Rossi

Tenebrio molitor meal represents a promising protein source for animal nutrition due to its low environmental impact and high nutritional value. To date, there is limited data in the literature regarding the effects of Tenebrio molitor meal on the modulation of gut microbiota in growing animals, with most results focusing on poultry rather than pigs. The aim of this study was to evaluate the effects of replacing fermented soy protein concentrate with Tenebrio molitor meal on gut microbiota and feed digestibility in growing pigs. A total of 14 growing pigs (80 ± 2 days old) were randomly allotted to two groups: the control group (CON) was fed a commercial diet containing 4% fermented soy protein concentrate (48% crude protein), and the treatment group (TM) was fed a basal diet containing 5% of T. molitor larvae meal formulated to be isonitrogenous and isoenergetic. The study lasted 28 days. Animals were weekly weighted and feed refuse was routinely measured. Fecal, blood samples, and rectal swabs were collected for analysis. No differences were observed in growth and diet digestibility for the protein and lipid components throughout the trial. No differences in the serum concentrations of albumin, globulin, urea, and interleukin-6 were registered in both groups, suggesting an unaltered health status. The TM group showed a significant difference in the beta diversity index considering the total duration of the trial (treatment effect evaluated with PERMANOVA, R2 0.0771, p value = 0.0099) showing an increased abundance of Elusimicrobium spp. and a decrease in Asteroplasma spp. in TM compared to the CON group (p < 0.05). Obtained findings indicate that 5% T. molitor meal can be included as a partial replacement for soy in growing pig formula without impairing pig growth and gut microbiota composition.

褐飞虱粉对环境影响小,营养价值高,是一种很有前景的动物营养蛋白质来源。迄今为止,有关褐飞虱粉对生长期动物肠道微生物群调节作用的文献数据还很有限,而且大多数结果都集中在家禽而不是猪身上。本研究的目的是评估用褐飞虱粉替代发酵大豆浓缩蛋白对生长猪肠道微生物群和饲料消化率的影响。研究人员将 14 头生长猪(80 ± 2 日龄)随机分为两组:对照组(CON)饲喂含有 4% 发酵大豆浓缩蛋白(粗蛋白含量为 48%)的商品日粮,治疗组(TM)饲喂含有 5% 的褐飞虱幼虫粉的基础日粮,该基础日粮为等氮等能配方。研究持续 28 天。每周给动物称重,并对饲料垃圾进行常规测量。收集粪便、血液样本和直肠拭子进行分析。在整个试验过程中,蛋白质和脂质成分的生长和日粮消化率均未出现差异。两组的白蛋白、球蛋白、尿素和白细胞介素-6 的血清浓度均无差异,表明健康状况没有改变。与 CON 组相比,TM 组在整个试验期间的 beta 多样性指数上有显著差异(用 PERMANOVA 评估治疗效果,R2 0.0771,p 值 = 0.0099),表明 TM 组的 Elusimicrobium 菌属数量增加,而 Asteroplasma 菌属数量减少(p 值 = 0.0099)。
{"title":"Microbiota modulation by the inclusion of Tenebrio molitor larvae as alternative to fermented soy protein concentrate in growing pigs diet.","authors":"Irene Ferri, Matteo Dell'Anno, Alessandro Quiese, Bianca Castiglioni, Paola Cremonesi, Filippo Biscarini, Benedetta Canala, Matteo Santoru, Alessia Colombini, Giancarlo Ruffo, Antonella Baldi, Luciana Rossi","doi":"10.1007/s11259-024-10588-6","DOIUrl":"https://doi.org/10.1007/s11259-024-10588-6","url":null,"abstract":"<p><p>Tenebrio molitor meal represents a promising protein source for animal nutrition due to its low environmental impact and high nutritional value. To date, there is limited data in the literature regarding the effects of Tenebrio molitor meal on the modulation of gut microbiota in growing animals, with most results focusing on poultry rather than pigs. The aim of this study was to evaluate the effects of replacing fermented soy protein concentrate with Tenebrio molitor meal on gut microbiota and feed digestibility in growing pigs. A total of 14 growing pigs (80 ± 2 days old) were randomly allotted to two groups: the control group (CON) was fed a commercial diet containing 4% fermented soy protein concentrate (48% crude protein), and the treatment group (TM) was fed a basal diet containing 5% of T. molitor larvae meal formulated to be isonitrogenous and isoenergetic. The study lasted 28 days. Animals were weekly weighted and feed refuse was routinely measured. Fecal, blood samples, and rectal swabs were collected for analysis. No differences were observed in growth and diet digestibility for the protein and lipid components throughout the trial. No differences in the serum concentrations of albumin, globulin, urea, and interleukin-6 were registered in both groups, suggesting an unaltered health status. The TM group showed a significant difference in the beta diversity index considering the total duration of the trial (treatment effect evaluated with PERMANOVA, R<sup>2</sup> 0.0771, p value = 0.0099) showing an increased abundance of Elusimicrobium spp. and a decrease in Asteroplasma spp. in TM compared to the CON group (p < 0.05). Obtained findings indicate that 5% T. molitor meal can be included as a partial replacement for soy in growing pig formula without impairing pig growth and gut microbiota composition.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"26"},"PeriodicalIF":1.8,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Novel approach for biomaterial assessment: utilizing the Ex Ovo quail cam assay for biocompatibility pre-screening. 生物材料评估的新方法:利用体外鹌鹑凸轮检测法进行生物相容性预筛选。
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-11-21 DOI: 10.1007/s11259-024-10574-y
Zuzana Tirpakova, Zuzana Demcisakova, Lenka Luptakova, Julia Hurnikova, Matus Coma, Lukas Urban, Peter Gal, Lubomir Medvecky, Eva Petrovova

In recent years, the chorioallantoic membrane (CAM) has emerged as a crucial component of biocompatibility testing for biomaterials designed for regenerative strategies and tissue engineering applications. This study explores angiogenic potential of an innovative acellular and porous biopolymer scaffold, based on polyhydroxybutyrate and chitosan (PHB/CHIT), using the ex ovo quail CAM assay as an alternative to the conventional chick CAM test. On embryonic day 6 (ED6), we placed the tested biomaterials on the CAM alone or soaked them with various substances, including vascular endothelial growth factor (VEGF-A), saline, or the endogenous angiogenesis inhibitor Angiostatin. After 72 h (ED9), we analyzed blood vessels formation, a sign of ongoing angiogenesis, in the vicinity of the scaffold and within its pores. We employed marker for cell proliferation (PHH3), embryonic endothelium (WGA, SNA), myofibroblasts (α-SMA), and endothelial cells (QH1) for morphological and histochemical analysis. Our findings demonstrated the robust angiogenic potential of the untreated scaffold without additional influence from the angiogenic factor VEGF-A. Furthermore, gene expression analysis revealed an upregulation of pro-angiogenic growth factors, including VEGF-A, ANG-2, and VE-Cadherin after 5 days of implantation, indicative of a pro-angiogenic microenvironment. These results underscore the inherent angiogenic potential of the PHB/CHIT composite. Additionally, monitoring of CAM microvilli growing to the scaffold provides a methodology for investigating the biocompatibility of materials using the ex ovo quail CAM assay as a suitable alternative model compared to the chicken CAM platform. This approach offers a rapid screening method for biomaterials in the field of tissue repair/regeneration and engineering.

近年来,绒毛膜(CAM)已成为再生策略和组织工程应用生物材料生物相容性测试的重要组成部分。本研究采用体外鹌鹑CAM试验替代传统的小鸡CAM试验,探讨了基于聚羟基丁酸酯和壳聚糖(PHB/CHIT)的创新性无细胞多孔生物聚合物支架的血管生成潜力。在胚胎第 6 天(ED6),我们将受测生物材料单独放在 CAM 上,或用各种物质浸泡,包括血管内皮生长因子(VEGF-A)、生理盐水或内源性血管生成抑制剂 Angiostatin。72 小时后(ED9),我们分析了支架附近和支架孔内的血管形成情况,这是血管持续生成的标志。我们采用细胞增殖标记物(PHH3)、胚胎内皮(WGA、SNA)、肌成纤维细胞(α-SMA)和内皮细胞(QH1)进行形态学和组织化学分析。我们的研究结果表明,未经处理的支架具有强大的血管生成潜能,而不会受到血管生成因子 VEGF-A 的额外影响。此外,基因表达分析表明,植入 5 天后,促血管生成生长因子(包括 VEGF-A、ANG-2 和 VE-Cadherin)上调,表明存在一个促血管生成的微环境。这些结果凸显了 PHB/CHIT 复合材料固有的血管生成潜力。此外,监测支架上生长的 CAM 微绒毛为研究材料的生物相容性提供了一种方法,与鸡 CAM 平台相比,使用体外鹌鹑 CAM 试验是一种合适的替代模型。这种方法为组织修复/再生和工程领域的生物材料提供了一种快速筛选方法。
{"title":"Novel approach for biomaterial assessment: utilizing the Ex Ovo quail cam assay for biocompatibility pre-screening.","authors":"Zuzana Tirpakova, Zuzana Demcisakova, Lenka Luptakova, Julia Hurnikova, Matus Coma, Lukas Urban, Peter Gal, Lubomir Medvecky, Eva Petrovova","doi":"10.1007/s11259-024-10574-y","DOIUrl":"10.1007/s11259-024-10574-y","url":null,"abstract":"<p><p>In recent years, the chorioallantoic membrane (CAM) has emerged as a crucial component of biocompatibility testing for biomaterials designed for regenerative strategies and tissue engineering applications. This study explores angiogenic potential of an innovative acellular and porous biopolymer scaffold, based on polyhydroxybutyrate and chitosan (PHB/CHIT), using the ex ovo quail CAM assay as an alternative to the conventional chick CAM test. On embryonic day 6 (ED6), we placed the tested biomaterials on the CAM alone or soaked them with various substances, including vascular endothelial growth factor (VEGF-A), saline, or the endogenous angiogenesis inhibitor Angiostatin. After 72 h (ED9), we analyzed blood vessels formation, a sign of ongoing angiogenesis, in the vicinity of the scaffold and within its pores. We employed marker for cell proliferation (PHH3), embryonic endothelium (WGA, SNA), myofibroblasts (α-SMA), and endothelial cells (QH1) for morphological and histochemical analysis. Our findings demonstrated the robust angiogenic potential of the untreated scaffold without additional influence from the angiogenic factor VEGF-A. Furthermore, gene expression analysis revealed an upregulation of pro-angiogenic growth factors, including VEGF-A, ANG-2, and VE-Cadherin after 5 days of implantation, indicative of a pro-angiogenic microenvironment. These results underscore the inherent angiogenic potential of the PHB/CHIT composite. Additionally, monitoring of CAM microvilli growing to the scaffold provides a methodology for investigating the biocompatibility of materials using the ex ovo quail CAM assay as a suitable alternative model compared to the chicken CAM platform. This approach offers a rapid screening method for biomaterials in the field of tissue repair/regeneration and engineering.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"24"},"PeriodicalIF":1.8,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11582168/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Demographic, morphological and coat factors in dogs after exercise at a fast course ability test (FCAT) trial. 狗在快速行进能力测试(FCAT)试验中运动后的人口、形态和皮毛因素。
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-11-21 DOI: 10.1007/s11259-024-10578-8
Ana Gabriela Jiménez, William Andrew Russel, Kailey Diane Paul, Alta McQuillen, Ahmet Ali Ay

Domestic dogs are a widely diverse species of endothermic mammals that show a positive correlation between body mass and whole-animal metabolic rate, but a negative correlation between body mass and lifespan, making them an interesting system for determining thermoregulatory patterns in relation to body mass, body morphology, and age within a single mammalian species. Though previous work has found differences in thermoregulation across seasons and with training in dogs of different sizes, we now seek to determine (1) whether sampling event-related temperature differences remained when dogs exercised intensely and acutely outdoors and (2) whether thermal differences were also expressed in short-term burst exercise in athletic dogs compared to long-term exercise in non-athletic dogs, as previously found. Here, we measured tympanic membrane temperature (Tear) as a correlate of core or internal body temperature (Tb). We also measured changes in body temperature across different body surfaces using thermal imaging (Teye, Tnose, and Tmouth) in dogs after exercise during Fast Course Agility Trial (FCAT) competitions between spring and summer months in Central New York State, USA (N = 20, July and August N = 26). We correlated these data to each dog's body mass (average(± standard error) = 29.97(± 0.24) lbs.), age (5.99(± 0.78) years), and various aspects of body part measurements and coat characteristics, such as length, type, and color. First, in our overall dataset, being sampled in May was the most significant predictor of temperature slope (p < 0.001), and we identified far more significant predictor variables in the May event dataset than in other datasets. Second, as we gave special attention to our study population, we found that running an outdoor, burst exercise course (FCAT trial) shows a different pattern of thermoregulation compared with previous work. Thus, our data may offer preliminary insights that thermoregulation in dogs varies with sampling event and exercise type, though additional research is needed to understand the complexity of these observed patterns. Our data also provides evidence that responses can be plastic depending on the dog's individual phenotype and that athleticism may affect thermoregulation in dogs, similar to humans.

家犬是一种种类繁多的内热哺乳动物,其体重与整只动物的新陈代谢率之间呈正相关,但体重与寿命之间呈负相关,这使家犬成为一种有趣的系统,可用于确定单一哺乳动物物种内与体重、身体形态和年龄相关的体温调节模式。尽管之前的研究发现不同体型的狗在不同季节和训练中的体温调节存在差异,但我们现在试图确定:(1)当狗在户外进行高强度的剧烈运动时,取样事件相关的体温差异是否仍然存在;(2)与之前发现的非运动型狗的长期运动相比,运动型狗的短期爆发性运动是否也表现出体温差异。在这里,我们测量了鼓膜温度(Tear)与核心或内部体温(Tb)的相关性。我们还利用热成像技术(Teye、Tnose 和 Tmouth)测量了狗在美国纽约州中部春季和夏季快速敏捷比赛(FCAT)期间运动后不同体表的体温变化(N = 20,7 月和 8 月 N = 26)。我们将这些数据与每只狗的体重(平均值(± 标准误差)= 29.97(± 0.24)磅)、年龄(5.99(± 0.78)岁)、身体部位测量值和皮毛特征(如长度、类型和颜色)等各方面相关联。首先,在我们的整个数据集中,5 月份采样是预测温度斜率最显著的因素(p
{"title":"Demographic, morphological and coat factors in dogs after exercise at a fast course ability test (FCAT) trial.","authors":"Ana Gabriela Jiménez, William Andrew Russel, Kailey Diane Paul, Alta McQuillen, Ahmet Ali Ay","doi":"10.1007/s11259-024-10578-8","DOIUrl":"https://doi.org/10.1007/s11259-024-10578-8","url":null,"abstract":"<p><p>Domestic dogs are a widely diverse species of endothermic mammals that show a positive correlation between body mass and whole-animal metabolic rate, but a negative correlation between body mass and lifespan, making them an interesting system for determining thermoregulatory patterns in relation to body mass, body morphology, and age within a single mammalian species. Though previous work has found differences in thermoregulation across seasons and with training in dogs of different sizes, we now seek to determine (1) whether sampling event-related temperature differences remained when dogs exercised intensely and acutely outdoors and (2) whether thermal differences were also expressed in short-term burst exercise in athletic dogs compared to long-term exercise in non-athletic dogs, as previously found. Here, we measured tympanic membrane temperature (T<sub>ear</sub>) as a correlate of core or internal body temperature (T<sub>b</sub>). We also measured changes in body temperature across different body surfaces using thermal imaging (T<sub>eye</sub>, T<sub>nose</sub>, and T<sub>mouth</sub>) in dogs after exercise during Fast Course Agility Trial (FCAT) competitions between spring and summer months in Central New York State, USA (N = 20, July and August N = 26). We correlated these data to each dog's body mass (average(± standard error) = 29.97(± 0.24) lbs.), age (5.99(± 0.78) years), and various aspects of body part measurements and coat characteristics, such as length, type, and color. First, in our overall dataset, being sampled in May was the most significant predictor of temperature slope (p < 0.001), and we identified far more significant predictor variables in the May event dataset than in other datasets. Second, as we gave special attention to our study population, we found that running an outdoor, burst exercise course (FCAT trial) shows a different pattern of thermoregulation compared with previous work. Thus, our data may offer preliminary insights that thermoregulation in dogs varies with sampling event and exercise type, though additional research is needed to understand the complexity of these observed patterns. Our data also provides evidence that responses can be plastic depending on the dog's individual phenotype and that athleticism may affect thermoregulation in dogs, similar to humans.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"25"},"PeriodicalIF":1.8,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metabolic and stress resistance effects in Macrobrachium rosenbergii juveniles supplemented with Curcuma longa extract. 补充莪术提取物的鲭鱼幼体的代谢和抗应激效应
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-11-20 DOI: 10.1007/s11259-024-10580-0
Ana Paula Pelinson da Fonseca, Milena Cia Retcheski, Luciano Tormen, Silvia Romão, Luisa Helena Cazarolli

This work studied the effects of C. longa extract as a dietary supplement for Macrobrachium rosenbergii. Shrimp juvenile (n = 960, initial average weight 0.06 ± 0.042 g and initial stocking density 0.4 animals/L) received diets with different concentrations of turmeric extract (0.05, 0.2, 1%) for 60 days, and zootechnical (weight gain, specific growth rate, initial and final condition factor) and metabolic (activities of digestive enzymes, amino acid metabolism enzymes, and energy metabolism enzymes) parameters were evaluated. A transport simulation (TS) test was conducted and, in challenged animals, survival and antioxidant status were studied. Phenolic compound content and in vitro antioxidant potential of the turmeric extract were evaluated. Turmeric extract (0.05%) significantly increased weight gain and the activity of digestive enzymes. An increase in citrate synthase activity for all concentrations of turmeric was also observed. The glutamate dehydrogenase activity in the 0.05% group was also increased by turmeric extract. The in vitro antioxidant activity of the turmeric extract was like butylated hydroxytoluene (BHT), a common antioxidant, and the total phenolic content was 0.98 ± 0.02 mmolAG/g. In animals from the TS test, turmeric extract promoted a dose-dependent increase in animal survival. Furthermore, turmeric supplementation increased the activity of the enzymes catalase, glutathione reductase, glutathione peroxidase, glutathione S-transferase, and it reduced glutathione (GSH) content. A reduction in the levels of lipid peroxidation (TBARS) and protein carbonyl was also observed in the hepatopancreas of all experimental groups. The addition of low concentrations of turmeric extract to the diet of M. rosenbergii juvenile presents great potential for application in commercial shrimp farming, since the extract (at concentrations as low as 0.05 to 1%) increased weight gain and improved the physiological and metabolic conditions of the animals, improving the use of nutrients and consequently the animals' responses to stressful conditions, such as transport.

这项工作研究了姜黄提取物作为一种膳食补充剂对大brachium rosenbergii的影响。幼虾(n = 960,初始平均体重为 0.06 ± 0.042 g,初始放养密度为 0.4 头/L)连续 60 天摄入含有不同浓度姜黄提取物(0.05%、0.2%、1%)的饲料,并对其动物技术(增重、特定生长率、初始和最终条件因子)和代谢(消化酶、氨基酸代谢酶和能量代谢酶的活性)参数进行了评估。进行了运输模拟(TS)试验,并研究了受挑战动物的存活率和抗氧化状态。评估了姜黄提取物的酚类化合物含量和体外抗氧化潜力。姜黄提取物(0.05%)显著提高了体重增加和消化酶的活性。还观察到所有浓度的姜黄都能提高柠檬酸合成酶的活性。姜黄提取物还提高了 0.05% 组的谷氨酸脱氢酶活性。姜黄提取物的体外抗氧化活性与常见的抗氧化剂丁基羟基甲苯(BHT)相似,总酚含量为 0.98 ± 0.02 mmolAG/g。在 TS 试验的动物中,姜黄提取物可促进动物存活率的增加,其增加与剂量有关。此外,姜黄还能提高过氧化氢酶、谷胱甘肽还原酶、谷胱甘肽过氧化物酶、谷胱甘肽 S-转移酶的活性,并降低谷胱甘肽(GSH)的含量。在所有实验组的肝胰腺中还观察到脂质过氧化物(TBARS)和蛋白质羰基水平的降低。在罗氏沼虾幼虾的饲料中添加低浓度姜黄提取物具有很大的商业化养虾潜力,因为姜黄提取物(浓度低至 0.05 至 1%)可提高增重,改善动物的生理和代谢状况,提高营养物质的利用率,从而改善动物对运输等应激条件的反应。
{"title":"Metabolic and stress resistance effects in Macrobrachium rosenbergii juveniles supplemented with Curcuma longa extract.","authors":"Ana Paula Pelinson da Fonseca, Milena Cia Retcheski, Luciano Tormen, Silvia Romão, Luisa Helena Cazarolli","doi":"10.1007/s11259-024-10580-0","DOIUrl":"10.1007/s11259-024-10580-0","url":null,"abstract":"<p><p>This work studied the effects of C. longa extract as a dietary supplement for Macrobrachium rosenbergii. Shrimp juvenile (n = 960, initial average weight 0.06 ± 0.042 g and initial stocking density 0.4 animals/L) received diets with different concentrations of turmeric extract (0.05, 0.2, 1%) for 60 days, and zootechnical (weight gain, specific growth rate, initial and final condition factor) and metabolic (activities of digestive enzymes, amino acid metabolism enzymes, and energy metabolism enzymes) parameters were evaluated. A transport simulation (TS) test was conducted and, in challenged animals, survival and antioxidant status were studied. Phenolic compound content and in vitro antioxidant potential of the turmeric extract were evaluated. Turmeric extract (0.05%) significantly increased weight gain and the activity of digestive enzymes. An increase in citrate synthase activity for all concentrations of turmeric was also observed. The glutamate dehydrogenase activity in the 0.05% group was also increased by turmeric extract. The in vitro antioxidant activity of the turmeric extract was like butylated hydroxytoluene (BHT), a common antioxidant, and the total phenolic content was 0.98 ± 0.02 mmolAG/g. In animals from the TS test, turmeric extract promoted a dose-dependent increase in animal survival. Furthermore, turmeric supplementation increased the activity of the enzymes catalase, glutathione reductase, glutathione peroxidase, glutathione S-transferase, and it reduced glutathione (GSH) content. A reduction in the levels of lipid peroxidation (TBARS) and protein carbonyl was also observed in the hepatopancreas of all experimental groups. The addition of low concentrations of turmeric extract to the diet of M. rosenbergii juvenile presents great potential for application in commercial shrimp farming, since the extract (at concentrations as low as 0.05 to 1%) increased weight gain and improved the physiological and metabolic conditions of the animals, improving the use of nutrients and consequently the animals' responses to stressful conditions, such as transport.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"20"},"PeriodicalIF":1.8,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Veterinary Research Communications
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1