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A modified withdrawal time estimation and risk assessment of enrofloxacin in grass carp (Ctenopharyngodon idella) after ad libitum medicated feed based on statistical approaches in natural cultured environments. 基于自然养殖环境中的统计方法,对草鱼(Ctenopharyngodon idella)自由采食药物饲料后恩诺沙星的停药时间估计和风险评估进行修正。
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-10-01 Epub Date: 2024-07-25 DOI: 10.1007/s11259-024-10475-0
Ning Xu, Huan Zhang, Jing Dong, Yibin Yang, Yongtao Liu, Shun Zhou, Xia Zhu, Xiaohui Ai

Enrofloxacin (EF) is a broad-spectrum and highly efficient antibiotic commonly used for treating diseases in aquatic animals. However, its abuse in aquaculture applications often leads to excess residue in tissues of grass carp (Ctenopharyngodon idella). Hence, this study aimed to estimate the withdrawal time (WT) of EF and its metabolite of ciprofloxacin (CF) administered medicated feed in natural culture environments and conduct a risk assessment. Plasma and tissue samples were gathered at appropriate time points and detected by high-performance liquid chromatography. The data homogeneity was evaluated by Bartlett's test and Cochran's test. The linearity of the regressed line was evaluated by visual inspection and F test. Outliers were estimated on a normal probability scale by plotting the standardized residual versus their cumulative frequency distribution. Finally, the WT was calculated to be 51 days in muscle + skin based on the maximum residue limit of 100 µg/kg. After 51 days, the concentration of EF and CF fell below 10 µg/kg. The estimated daily intake was calculated to be 0.009 µg/kg/d. Hazard quotient was computed to be 0.002, which was far below one. These results suggested that calculated WT of EF could ensure the safety of products from grass carp for humans.

恩诺沙星(EF)是一种广谱高效抗生素,常用于治疗水生动物疾病。然而,其在水产养殖中的滥用往往会导致草鱼(Ctenopharyngodon idella)组织中的残留超标。因此,本研究旨在估算环丙沙星(CF)药物饲料中 EF 及其代谢物在自然养殖环境中的停药时间(WT),并进行风险评估。在适当的时间点采集血浆和组织样本,并采用高效液相色谱法进行检测。通过巴特利特检验和科克兰检验评估了数据的同质性。回归线的线性度通过目测和 F 检验进行评估。通过绘制标准化残差与其累积频率分布图,按照正态概率标度估算异常值。最后,根据 100 微克/千克的最大残留限量,计算出肌肉和皮肤中的 WT 为 51 天。51 天后,EF 和 CF 的浓度降至 10 微克/千克以下。经计算,估计日摄入量为 0.009 微克/千克/天。计算得出的危害商为 0.002,远远低于 1。这些结果表明,计算出的 EF WT 可确保草鱼产品对人类的安全性。
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引用次数: 0
Hexaflumuron insecticide exposure induces behavior alterations, hemato-biochemical disorders, antioxidant-immune dysfunction, and histopathological alterations in Nile tilapia (Oreochromis niloticus). 接触六氟隆杀虫剂会诱发尼罗罗非鱼(Oreochromis niloticus)的行为改变、血液生化紊乱、抗氧化免疫功能障碍和组织病理学改变。
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-10-01 Epub Date: 2024-07-31 DOI: 10.1007/s11259-024-10467-0
Rowida E Ibrahim, Moustafa M S Fouda, Abdelwahab A Abdelwarith, Elsayed M Younis, Eman Wagih, Basma A Elshafey, Simon J Davies, Afaf N Abdel Rahman

Hexaflumuron (HEX) insecticide is widely used in agriculture practices to fight crop insects. The toxicological effect of HEX on Nile tilapia (Oreochromis niloticus) was investigated in this study. Two hundred and forty fish (35.50 ± 1.45 g) were divided into six groups in four replicates (40 fish/group; 10 fish/replicate) and were exposed to six distinct HEX concentrations (0, 2, 4, 6, 8, and 10 mg L-1) for 96-h. The 96-h lethal concentration 50 (96-h LC50) of HEX was calculated to be 7.19 mg L-1. The fish exhibited reduced surface and middle swimming, aggressiveness, and tail-spreading behaviors with increasing bottom swimming and resting patterns after HEX exposure. HEX exposure resulted in body bleeding and fin rot. The erythrogram (red blood cell count, hemoglobin, and packed cell volume %) was significantly reduced with increased mean corpuscular volume by HEX exposure. HEX exposure decreased the white blood cells (WBCs) and differential WBC counts. Acute HEX exposure raised 8-hydroxy-2-deoxyguanosine level while lowering brain acetylcholine esterase activity. HEX exposure caused hepato-renal dysfunction and increased stress-related parameters (glucose and cortisol). Exposure to HEX reduced the immune responses (lysozyme, nitric oxide, immunoglobulin M, and complement 3). A substantial decrease in the antioxidant variables (reduced glutathione content and catalase) with increasing the malondialdehyde was noted by HEX exposure. Moreover, histopathological changes resulted from HEX exposure in the gills, liver, kidney, and spleen. These results indicate that HEX exposure induced behavioral changes, hepato-renal dysfunction, and immune-antioxidant disruption, indicating a possible physiological disruption in O. niloticus.

六氟磺隆(HEX)杀虫剂被广泛应用于农业生产中防治农作物害虫。本研究调查了六氟磺隆对尼罗罗非鱼(Oreochromis niloticus)的毒理学影响。将 240 尾鱼(35.50 ± 1.45 克)分成 6 组,每组 40 尾,每组 10 尾,共 4 个重复,暴露于 6 种不同浓度的六六六(0、2、4、6、8 和 10 毫克/升-1)中 96 小时。经计算,96 小时的 HEX 致死浓度 50(96-h LC50)为 7.19 mg L-1。暴露于 HEX 后,鱼类的表层和中层游动、攻击性和展尾行为减少,底层游动和静止行为增加。暴露于 HEX 会导致鱼体出血和鳍腐烂。接触六六六后,红细胞图(红细胞计数、血红蛋白和充盈细胞体积百分比)显著减少,平均血球容积增加。接触六六六会降低白细胞(WBCs)和白细胞差值计数。急性接触六六六会提高 8-羟基-2-脱氧鸟苷水平,同时降低脑乙酰胆碱酯酶活性。暴露于六羟环己烷会导致肝肾功能障碍,并增加应激相关参数(葡萄糖和皮质醇)。暴露于 HEX 会降低免疫反应(溶菌酶、一氧化氮、免疫球蛋白 M 和补体 3)。暴露于 HEX 后,抗氧化变量(还原型谷胱甘肽含量和过氧化氢酶)大幅减少,丙二醛含量增加。此外,接触过氧化氢还导致鱼鳃、肝脏、肾脏和脾脏发生组织病理学变化。这些结果表明,接触六六六会诱发行为变化、肝肾功能障碍和免疫抗氧化紊乱,表明尼罗河鱼可能存在生理紊乱。
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引用次数: 0
Duddingtonia flagrans and its crude proteolytic extract: concomitant action in sheep coprocultures. Duddingtonia flagrans 及其粗蛋白水解提取物:在绵羊共育中的协同作用。
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-10-01 Epub Date: 2024-08-09 DOI: 10.1007/s11259-024-10494-x
Debora Castro de Souza, Elias Honorato Gomes, Lisseth Bibiana Figueroa Puentes, Dásia Silveira Soares, Daniele Vieira da Silva, Laísa Bastos Albuquerque, Pedro Henrique Dutra Dos Santos, Tiago Moreira Facury, Fabio Ribeiro Braga, Filippe Elias de Freitas Soares

The presence of infective larvae (L3) of gastrointestinal nematode (GIN) parasites in pastures directly contributes to the constant recurrence of infections in ruminant herds. This study aimed to evaluate the nematophagous fungus Duddingtonia flagrans (AC001) (proteolytic crude extract and/or conidia) in the in vitro control of GIN L3 in coprocultures. To produce the proteolytic crude extract, a suspension (107 conidia/mL) of D. flagrans was inoculated into a liquid medium. After 6 days, the medium was filtered, centrifuged, and its proteolytic activity was measured. For the experimental assay, fecal samples were collected directly from the rectal ampulla of naturally infected sheep, and egg counts per gram of feces (EPG) were performed. Coprocultures were prepared using 10 g of fecal material with the groups defined as follows: control group G1 (1.0 mL of denatured proteolytic crude extract); treated group G2 (1.0 mL of active proteolytic crude extract); treated group G3 (1.0 mL of active proteolytic crude extract + 1.0 mL of AC001 conidia). The coprocultures were maintained at room temperature (25ºC), for 7 days, and then the L3 larvae were recovered. The results demonstrated that AC001 successfully produced protease (56.34 U/mL). The treatments with active proteolytic crude extract (G2) and active proteolytic crude extract + AC001 conidia (G3) were significantly different (p < 0.01) from the control group with denatured proteolytic crude extract (G1). AC001 and its proteolytic crude extract acted concomitantly on helminths directly in the fecal environment, suggesting potential future applications in the field.

牧场中胃肠道线虫(GIN)寄生虫感染性幼虫(L3)的存在直接导致了反刍动物群感染的不断复发。本研究旨在评估食线虫真菌 Duddingtonia flagrans (AC001)(蛋白水解粗提取物和/或分生孢子)在体外共培养中对 GIN L3 的控制作用。为了生产蛋白水解粗提取物,将 D. flagrans 的悬浮液(107 个分生孢子/毫升)接种到液体培养基中。6 天后,过滤培养基并离心,测量其蛋白水解活性。在实验测定中,直接从自然感染绵羊的直肠安瓿中收集粪便样本,并进行每克粪便中的虫卵计数(EPG)。使用 10 克粪便材料制备共培养物,各组定义如下:对照组 G1(1.0 毫升变性蛋白水解粗提取物);处理组 G2(1.0 毫升活性蛋白水解粗提取物);处理组 G3(1.0 毫升活性蛋白水解粗提取物 + 1.0 毫升 AC001 分生孢子)。共培养物在室温(25ºC)下保持 7 天,然后回收 L3 幼虫。结果表明,AC001 成功地产生了蛋白酶(56.34 U/mL)。活性蛋白水解粗提物处理(G2)和活性蛋白水解粗提物 + AC001 分生孢子处理(G3)有显著差异(p
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引用次数: 0
Autochthonous probiotic bacteria improve intestinal pathology and histomorphology, expression of immune and growth-related genes and resistance against Vibrio alginolyticus in Asian seabass (Lates calcarifer). 自生益生菌可改善亚洲鲈鱼(Lates calcarifer)的肠道病理学和组织形态学、免疫和生长相关基因的表达以及对藻类溶解弧菌的抵抗力。
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-10-01 Epub Date: 2024-08-16 DOI: 10.1007/s11259-024-10502-0
Seyyad Mojtaba Emam, Babak Mohammadian, Takavar Mohammadian, Mohammad Reza Tabande

The study isolated two strains of intestinal autochthonous bacteria Lactiplantibacillus plantarum1 (MH155966.1) (L1) and Lactiplantibacillus plantarum2 (MH105076.1) (L2) from the Choobdeh Abadan region. The aim of this study was to investigate the effects of different strains of probiotic bacteria on the growth performance, digestive enzyme activity, histopathologic and histomorphometric characterization of the intestine, expression of immune and growth related genes, and evaluate Lates calcarifer resistance against Vibrio alginolyticus. To achieve this, for each treatment 60 L. calcarifer juveniles (75 ± 12 g) were randomly distributed in three fiberglass tanks (300 L) and fed for 45 days. The treatments were established as Diet 1 (control diet); L1 (diet with Lb. plantarum isolated 1); L2 (diet with Lb. plantarum isolated 2) with a bacterial concentration of 1 × 109 CFU/g. Nine fish from each treatment were sampled and examined, after euthanasia. The fish were placed 2 cm from the beginning of the intestine for microscopic sampling of villi height, villi width and thickness of the epithelium, with 3 treatments: The result showed differences in the mean values of total weight were found at the end of the experiment. After 45 days of culture, the fish fed with L1 had higher (P < 0.05) growth performance than the other treatment groups. But at the end of the trial, in L2, the digestive enzyme activities were higher (P < 0.05) than the other treatment groups. The fishes fed diets supplemented with the L2 group, like the digestive enzyme activities test, presented an increase in the thickness of the epithelium of the intestine, and villus height, and villus width were greatest in L2. Fish feeding with L1 and L2 probiotics induced higher transcription levels of interleukin-10 (IL-10), granulocyte-macrophage colony-forming cells (GMCFC), epidermal growth factor (EGF), and Transforming Growth Factor Beta (TGF-β) genes in the gut, which may correlate with better immune and hematological parameters in these groups. The results of the challenge test revealed that the percentage of survival was significantly higher in L1 (76.2%) and L2 (80.95%) treatments than in the control (P < 0.05). These results indicate that host-derived probiotics (Lb. plantarum) have significant potential as important probiotics to enhance nutrient utilization, Digestive enzymes, and metabolism by increasing the gut surface area of Lates calcarifer juveniles at 45 days of culture.

该研究从乔布德阿巴丹地区分离出两株肠道自生菌植物乳杆菌1(MH155966.1)(L1)和植物乳杆菌2(MH105076.1)(L2)。本研究的目的是调查不同益生菌株对鲈鱼生长性能、消化酶活性、肠道组织病理学和组织形态计量学特征、免疫和生长相关基因表达的影响,并评估鲈鱼对藻类溶解弧菌的抗性。为此,在每种处理中,将 60 尾金目鲈幼鱼(75 ± 12 克)随机分配到三个玻璃纤维水槽(300 升)中,喂养 45 天。处理方法分别为:1 号饮食(对照饮食);L1 号饮食(含分离出的植物鳞状芽孢杆菌 1 号饮食);L2 号饮食(含分离出的植物鳞状芽孢杆菌 2 号饮食),细菌浓度为 1 × 109 CFU/g。安乐死后,对每种处理的九条鱼进行取样和检查。将鱼放置在距离肠道起点 2 厘米处,用显微镜对 3 种处理的绒毛高度、绒毛宽度和上皮厚度进行取样:结果表明,实验结束时总重量的平均值存在差异。养殖 45 天后,投喂 L1 的鱼的体重(P
{"title":"Autochthonous probiotic bacteria improve intestinal pathology and histomorphology, expression of immune and growth-related genes and resistance against Vibrio alginolyticus in Asian seabass (Lates calcarifer).","authors":"Seyyad Mojtaba Emam, Babak Mohammadian, Takavar Mohammadian, Mohammad Reza Tabande","doi":"10.1007/s11259-024-10502-0","DOIUrl":"10.1007/s11259-024-10502-0","url":null,"abstract":"<p><p>The study isolated two strains of intestinal autochthonous bacteria Lactiplantibacillus plantarum1 (MH155966.1) (L1) and Lactiplantibacillus plantarum2 (MH105076.1) (L2) from the Choobdeh Abadan region. The aim of this study was to investigate the effects of different strains of probiotic bacteria on the growth performance, digestive enzyme activity, histopathologic and histomorphometric characterization of the intestine, expression of immune and growth related genes, and evaluate Lates calcarifer resistance against Vibrio alginolyticus. To achieve this, for each treatment 60 L. calcarifer juveniles (75 ± 12 g) were randomly distributed in three fiberglass tanks (300 L) and fed for 45 days. The treatments were established as Diet 1 (control diet); L1 (diet with Lb. plantarum isolated 1); L2 (diet with Lb. plantarum isolated 2) with a bacterial concentration of 1 × 10<sup>9</sup> CFU/g. Nine fish from each treatment were sampled and examined, after euthanasia. The fish were placed 2 cm from the beginning of the intestine for microscopic sampling of villi height, villi width and thickness of the epithelium, with 3 treatments: The result showed differences in the mean values of total weight were found at the end of the experiment. After 45 days of culture, the fish fed with L1 had higher (P < 0.05) growth performance than the other treatment groups. But at the end of the trial, in L2, the digestive enzyme activities were higher (P < 0.05) than the other treatment groups. The fishes fed diets supplemented with the L2 group, like the digestive enzyme activities test, presented an increase in the thickness of the epithelium of the intestine, and villus height, and villus width were greatest in L2. Fish feeding with L1 and L2 probiotics induced higher transcription levels of interleukin-10 (IL-10), granulocyte-macrophage colony-forming cells (GMCFC), epidermal growth factor (EGF), and Transforming Growth Factor Beta (TGF-β) genes in the gut, which may correlate with better immune and hematological parameters in these groups. The results of the challenge test revealed that the percentage of survival was significantly higher in L1 (76.2%) and L2 (80.95%) treatments than in the control (P < 0.05). These results indicate that host-derived probiotics (Lb. plantarum) have significant potential as important probiotics to enhance nutrient utilization, Digestive enzymes, and metabolism by increasing the gut surface area of Lates calcarifer juveniles at 45 days of culture.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141989015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The first evidence of Asian-like CPV-2b in Slovakia in a vaccinated dog with an acute fatal course of parvovirus infection: a case report. 斯洛伐克首次发现亚洲样 CPV-2b 的证据:病例报告,一只接种过疫苗的狗出现了急性致命性细小病毒感染病程。
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-10-01 Epub Date: 2024-08-09 DOI: 10.1007/s11259-024-10492-z
Andrea Pelegrinová, Patrícia Petroušková, Ľuboš Korytár, Anna Ondrejková, Monika Drážovská, Boris Vojtek, Jana Mojžišová, Marián Prokeš, Maroš Kostičák, Ľubica Zákutná, Michal Dolník, René Mandelík

This study provides a comprehensive description of the clinical course of a fatal parvovirus infection in a vaccinated dachshund puppy, along with the first identification of a new CPV-2 variant in Slovakia, elucidated through molecular amino acid analysis of the VP2 gene. The dog exhibited clinical signs such as apathy, vomiting, and bloody diarrhea. After confirming CPV-2 infection with a commercial snap test, intensive therapy was initiated. The dog succumbed within 48 h of admission. A rectal swab sample was collected, CPV-2 was examined using the PCR method, and sequenced. The virus detected in the patient was related to strains of CPV-2c of Asian origin and unrelated to European CPV-2b strains. The sequence had genetic signatures typical of Asian strains (VP2: 5Gly, 267Tyr, 324Ile, 370Arg, and 440Thr). Phylogenetic analysis classified this strain as similar to Asian strains of CPV-2c. It is believed to be derived from an Asian strain similar to CPV-2c that acquired the 426Asp mutation. With this finding, we present the first evidence of an Asian-like CPV-2b strain in the territory of Slovakia.

本研究全面描述了一只接种过疫苗的腊肠犬幼犬感染致命性副病毒的临床过程,并通过对 VP2 基因进行分子氨基酸分析,首次在斯洛伐克发现了一种新的 CPV-2 变体。该犬表现出冷漠、呕吐和血性腹泻等临床症状。在通过商用快速检测确认 CPV-2 感染后,开始了强化治疗。该犬在入院 48 小时内死亡。采集了直肠拭子样本,使用 PCR 方法检测了 CPV-2,并进行了测序。在患者体内检测到的病毒与源自亚洲的 CPV-2c 株系有关,与欧洲的 CPV-2b 株系无关。病毒序列具有典型的亚洲毒株基因特征(VP2:5Gly、267Tyr、324Ile、370Arg 和 440Thr)。系统发育分析将该菌株归类为与亚洲 CPV-2c 株系相似的菌株。据信,它来自于获得 426Asp 突变的与 CPV-2c 相似的亚洲毒株。我们的这一发现首次证明了斯洛伐克境内存在类似亚洲的 CPV-2b 株系。
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引用次数: 0
Early Detection and Management of Lamanema chavezi infection in a llama (Lama glama) in Switzerland. 瑞士骆驼(Lama glama)感染拉曼莫氏菌(Lamanema chavezi)的早期检测和管理。
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-10-01 Epub Date: 2024-07-16 DOI: 10.1007/s11259-024-10465-2
Diana S Gliga, Anne Kramer, Gastón Moré, Caroline F Frey, Walter Basso

Lamanema chavezi is an entero-hepatic strongylid parasite specific to South American camelids. It has been reported only on few occasions outside South America. Due to its hepatic migration, it can cause extensive liver damage, leading to granulomatous and fibrotic hepatitis and manifesting with lethargy, anorexia, and even death. We are reporting the second case of L. chavezi infection in Europe and the first in Switzerland. The patient was a three-year old neutered male llama (Lama glama). Clinical examination revealed bloody mucous discharge from the anus. Fecal sedimentation/flotation revealed strongylid eggs consistent with L. chavezi, which were molecularly confirmed by a PCR targeting the ITS2 plus 5.8S and 28S rDNA flanking regions and amplicon sequencing. Eighteen weeks after administration of a single dose of eprinomectin (0.2 mg/kg i.m.), no further L. chavezi eggs were detected in the feces. The source of infection could not be traced back. The entire herd consisted of llamas bred in Switzerland. L. chavezi has been rarely reported outside South America, but its potential for pathogenicity and establishment should not be underestimated. Fecal sedimentation/flotation techniques should be routinely performed to ensure early detection of the parasite.

Lamanema chavezi 是南美洲驼科动物特有的一种肠肝强直性寄生虫。在南美洲以外的地区,只有少数几次报道过这种寄生虫。由于其肝脏迁移性,它可造成广泛的肝损伤,导致肉芽肿性和纤维性肝炎,表现为嗜睡、厌食甚至死亡。我们报告的是欧洲第二例、瑞士第一例 L. chavezi 感染病例。患者是一只三岁大的阉割雄性骆驼(Lama glama)。临床检查发现肛门有血性粘液分泌物。粪便沉淀/浮选发现了与L. chavezi一致的强直虫卵,通过针对ITS2加上5.8S和28S rDNA侧翼区的PCR和扩增子测序进行了分子确证。施用单剂量的伊普瑞林(0.2 mg/kg i.m.)18周后,粪便中没有再检测到L. chavezi虫卵。感染源无法追溯。整个骆驼群由在瑞士饲养的骆驼组成。L. chavezi 在南美洲以外的地区鲜有报道,但其潜在的致病性和确立性不容低估。应定期进行粪便沉淀/浮选技术,以确保及早发现寄生虫。
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引用次数: 0
Life satisfaction, psychological distress, compassion satisfaction and resilience: when the pleasure of helping others protects veterinary staff from emotional suffering. 生活满意度、心理困扰、同情满意度和复原力:当助人为乐保护兽医员工免受情感痛苦时。
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-10-01 Epub Date: 2024-08-20 DOI: 10.1007/s11259-024-10510-0
Maria Manuela Peixoto, Olga Cunha

Introduction: Individuals working in veterinary field suffer significantly from mental health problems, and research has extensively focused on psychological and work-related predictors of psychological distress. This study intended to approach psychological distress through a positive lens by investigating the predictive role of life satisfaction on psychological distress in veterinary staff, and the mediating effect of compassion satisfaction, resilience and perceived social support.

Methodology: A total of 868 veterinary staff (i.e. veterinarians, veterinary nurses, veterinary assistants and veterinary administrative staff) completed a web-survey assessing life satisfaction, psychological distress, compassion satisfaction, resilience, and social support.

Results: Life satisfaction negatively predicts psychological distress, and compassion satisfaction and resilience showed a mediation effect on the relationship between life satisfaction and psychological distress, with compassion satisfaction explaining 59% and resilience 6.4% of the effect of life satisfaction on psychological distress.

Conclusion: Current data support the role of life satisfaction as a protective dimension on psychological distress within a broader sample population of veterinary staff in Portugal, highlighting the role of compassion satisfaction and resilience in contributing in minimising distress among veterinary staff.

简介在兽医领域工作的人深受心理健康问题的困扰,研究广泛关注心理和工作相关的心理困扰预测因素。本研究旨在从积极的角度探讨生活满意度对兽医工作人员心理困扰的预测作用,以及同情满意度、复原力和感知社会支持的中介效应:共有868名兽医工作人员(即兽医、兽医护士、兽医助理和兽医行政人员)完成了一项网络调查,对生活满意度、心理困扰、同情满意度、复原力和社会支持进行了评估:生活满意度对心理困扰具有负向预测作用,同情满意度和复原力对生活满意度和心理困扰之间的关系具有中介效应,同情满意度和复原力分别解释了生活满意度对心理困扰影响的59%和6.4%:目前的数据支持生活满意度在更广泛的葡萄牙兽医工作人员样本人群中作为心理困扰的保护维度的作用,强调了同情满意度和恢复力在减少兽医工作人员心理困扰方面的作用。
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引用次数: 0
Pharmabiotic/phytobiotic formulas approach and their intravaginal effect on different parameters. 药用生物/植物生物配方及其对不同参数的阴道内影响。
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-10-01 Epub Date: 2024-07-09 DOI: 10.1007/s11259-024-10450-9
María Hortencia Miranda, María Elena Fátima Nader-Macías

Postpartum reproductive infections in cows generate significant economic losses. The use of lactic acid bacteria in animal health is an alternative tool to avoid antibiotic therapy in the prevention/treatment of bovine reproductive infections. In previous studies, 6 lactic bacteria from bovine mammary glands and vagina with beneficial, safe and technological characteristics were selected, and included in probiotic/phytobiotic formulas (combined with Malva and Lapacho extracts). In this work, probiotic and phytobiotic formulations were designed and their long-term viability determined. They were administered intravaginally to 30 females pregnant bovine pre and postpartum. The modification of the native microbiota and permanence/colonization of cultivable bacteria was evaluated, and also the safety of the designed products through the application of nutritional, clinical, hematological and biochemical parameters. The microorganisms maintained their viability up to 9 months at refrigeration temperature. The number of cultivable bacteria showed different pattern: total aerobic mesophylls increased slightly in all experimental groups, while Enterobacteriaceae increased after delivery, except in beneficial acid lactic bacteria + vegetable extract cows. Control and vegetable extract females showed the highest numbers of Enterobacteriaceae at the end of the trial (30 days postpartum). The number of lactic acid bacteria increased significantly in all the groups between 15 days pre and postpartum. The different parameters evaluated demonstrate the safety and harmlessness of the designed formulas, without producing local and systemic adverse effects in the cows.

奶牛产后生殖感染会造成重大经济损失。在动物保健中使用乳酸菌是预防/治疗牛生殖感染时避免使用抗生素治疗的一种替代工具。在之前的研究中,从牛乳腺和阴道中筛选出了 6 种有益、安全且具有技术特点的乳酸菌,并将其纳入益生菌/植物抗生素配方(与麦芽和腊肠提取物结合使用)。在这项工作中,设计了益生菌和植物生物配方,并测定了它们的长期存活率。在产前和产后,对 30 头雌性怀孕牛进行阴道内给药。通过营养、临床、血液学和生化参数的应用,对本地微生物群的改变和可培养细菌的持久性/定殖进行了评估,并对所设计产品的安全性进行了评估。微生物在冷藏温度下的存活时间长达 9 个月。可培养细菌的数量呈现出不同的模式:除有益酸性乳酸菌+蔬菜提取物奶牛外,所有实验组的需氧中球菌总数都略有增加,而肠杆菌科在分娩后有所增加。在试验结束时(产后 30 天),对照组和蔬菜提取物组母牛的肠杆菌数量最多。在产前 15 天和产后 15 天之间,所有组别的乳酸菌数量都有显著增加。所评估的不同参数表明,所设计的配方安全无害,不会对奶牛的局部和全身产生不良影响。
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引用次数: 0
Ulcerative and pyogranulomatous pododermatitis due to Pseudomonas luteola infection in a domestic ferret (Mustela putorius furo): a case report with literature review of this emerging zoonotic disease in ferrets. 家养雪貂(Mustela putorius furo)感染假单胞菌(Pseudomonas luteola)引起的溃疡性和脓毒血症性荚膜皮炎:一份病例报告,并附有关于这种新出现的雪貂人畜共患疾病的文献综述。
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-10-01 Epub Date: 2024-07-23 DOI: 10.1007/s11259-024-10464-3
Jacobo Giner, María Eugenia Lebrero, Diego López-Sahuquillo, Sergio Villanueva-Saz, Carles Juan-Sallés, Teresa Navarro, Antonio Fernández, Diana Marteles, Álex Gómez

Pseudomonas luteola (P.luteola), formerly called Chryseomonas luteola, is a strict aerobic gram-negative bacillus, 0.8 to 1.0 µm wide and 1.5 to 2.5 µm long, considered an opportunistic pathogen found ubiquitously in humid environments, both in soil and water. It sporadically causes disease in animals and immunosuppressed humans or those subjected to invasive procedures such us peritoneal dialysis or catheterization. In ferrets, this infection was first described in Spain in 2012 and since then, cases have appeared occasionally in Finland, Austria, Australia, France, the United States and also in Spain. This pathogen is considered an emerging zoonotic disease in ferrets, causing respiratory disease, panniculitis, and abscesses due to pyogranulomatous or suppurative inflammation predominantly of the pleura, lung, mediastinum, panniculus or salivary glands, frequently with lethal consequences. The clinical case of a ferret, infected by Pseudomona luteola, presenting with ulcerative suppurative pododermatitis and ipsilateral popliteal purulent lymphadenitis, is described. Together with a complete resolution of the clinical case by means of a non-invasive medical management likely due to the rapid detection, identification, and treatment of the infection.

卢特氏假单胞菌(P.luteola)原名为卢特氏奇异单胞菌(Chryseomonas luteola),是一种严格需氧的革兰氏阴性杆菌,宽 0.8 至 1.0 微米,长 1.5 至 2.5 微米,被认为是一种机会性病原体,普遍存在于潮湿的土壤和水中。它偶尔会在动物、免疫抑制的人或接受腹膜透析或导管插入等侵入性手术的人中致病。2012 年,西班牙首次描述了雪貂感染该病原体的情况,此后,芬兰、奥地利、澳大利亚、法国、美国和西班牙也偶尔出现病例。这种病原体被认为是雪貂间一种新出现的人畜共患病,主要在胸膜、肺部、纵隔、丹田或唾液腺等部位引起化脓性或化脓性炎症,导致呼吸道疾病、丹田炎和脓肿,经常造成致命后果。本病例描述了一只雪貂受假丝酵母菌感染后出现溃疡性化脓性足皮炎和同侧腘窝化脓性淋巴结炎的临床病例。该病例通过非侵入性医疗手段得到了彻底解决,这很可能归功于对感染的快速检测、识别和治疗。
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引用次数: 0
Genotype classification and pathogenicity of infectious bursal disease virus circulating in vaccinated broiler chicken farms. 疫苗肉鸡养殖场中流行的传染性法氏囊病病毒的基因型分类和致病性。
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-10-01 Epub Date: 2024-07-30 DOI: 10.1007/s11259-024-10468-z
Samah M Mosad, Mona M Elsayed, Enas M Hammad, Basma M Hendam, Hanaa S Ali, Abdelfattah H Eladl, Mohamed A Saif

This study investigated the genotype classification and pathogenicity of infectious bursal disease virus (IBDV) circulating in vaccinated broiler chicken farms in Egypt. A total of 150 samples were collected from 30 vaccinated commercial broiler chicken farms and pooled into 30 working samples. IBDV was tested using reverse transcriptase polymerase chain reaction (RT-PCR) amplification of the hypervariable region of the viral protein 2 (hvVP2) and the VP1 gene 5' extremity. Both RT-PCR fragments were sequenced from six samples, and then the obtained nucleotide sequences were analyzed. The IBDV genotypes were identified using nucleotide sequences. Five sequences of the six strains examined were classified as genotype A3B2 for the highly virulent segments A and B (vv-A/vv-B IBDV). Interestingly, this study identified and classified a novel segment-reassortant strain as the A1B2 genotype. Specifically, it involved the segment reassortment of classical virulent segment A (cv-A) with vv-B producing cv-A/vv-B reassortant IBDV. Subsequently, we compared the pathogenicity of reassortant (cv-A/vv-B) IBDV and vvIBDV strains identified in this study. Both strains developed typical IBD clinical signs, postmortem lesions, histopathology, immunohistochemistry, and lesion scores, which were more severe in vvIBDV than reassortant IBDV. In conclusion, this is the first report of the genotype classification based on both genome segments (hvVP2 and VP1) with pathogenicity of IBDV circulating in vaccinated broiler chicken farms and this pathogenicity is more severe in vvIBDV strain than a novel reassortant IBDV strain.

本研究调查了埃及疫苗肉鸡养殖场中流行的传染性法氏囊病病毒(IBDV)的基因型分类和致病性。研究人员从 30 个接种过疫苗的商业肉鸡养殖场共收集了 150 份样本,并将其集中为 30 份工作样本。采用逆转录酶聚合酶链反应(RT-PCR)扩增病毒蛋白 2 的超变异区(hvVP2)和 VP1 基因 5' 末端来检测 IBDV。对六个样本中的两个 RT-PCR 片段进行了测序,然后对获得的核苷酸序列进行了分析。通过核苷酸序列确定了 IBDV 基因型。所检测的六株菌株中有五株的序列被归类为基因型 A3B2,即高致病性片段 A 和 B(vv-A/vv-B IBDV)。有趣的是,这项研究发现了一种新的不重要片段毒株,并将其归类为 A1B2 基因型。具体地说,它涉及经典毒性片段 A(cv-A)与 vv-B 的片段重配,产生了 cv-A/vv-B 重配 IBDV。随后,我们比较了重变种(cv-A/vv-B)IBDV 和本研究中发现的 vvIBDV 株系的致病性。两种毒株都会出现典型的 IBD 临床症状、死后病变、组织病理学、免疫组化和病变评分,其中 vvIBDV 比重交 IBDV 更加严重。总之,这是首次报道基于两个基因组片段(hvVP2 和 VP1)对疫苗肉鸡养殖场中流行的 IBDV 的致病性进行基因型分类,而且 vvIBDV 株的致病性比新型重交 IBDV 株更严重。
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引用次数: 0
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Veterinary Research Communications
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