{"title":"[The significance of glucose for the growth of Yoshida ascites tumor cells in suspension cultures].","authors":"R Averdunk, E Liss","doi":"10.1515/znb-1971-0619","DOIUrl":"https://doi.org/10.1515/znb-1971-0619","url":null,"abstract":"","PeriodicalId":23706,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete","volume":"26 6","pages":"595-8"},"PeriodicalIF":0.0,"publicationDate":"1971-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znb-1971-0619","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15499526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The hypothesis of cancerostatic activity of deoxycholic acid, recently outlined, is supported by correlation of natural cancer resistance with the level of deoxycholic acid in animals and humans. Analyses of sera indicate lower levels of unconjugated deoxycholic acid in cancer patients, the mean values of other bile acids being normal. Application of this theory to statistics of cancer incidence reveals possibilities of new aspects.
{"title":"Evidence of the participation of deoxycholate in cancer immunity.","authors":"B Vlcek, A Reif, B Seidlová","doi":"10.1515/znb-1971-0509","DOIUrl":"https://doi.org/10.1515/znb-1971-0509","url":null,"abstract":"The hypothesis of cancerostatic activity of deoxycholic acid, recently outlined, is supported by correlation of natural cancer resistance with the level of deoxycholic acid in animals and humans. Analyses of sera indicate lower levels of unconjugated deoxycholic acid in cancer patients, the mean values of other bile acids being normal. Application of this theory to statistics of cancer incidence reveals possibilities of new aspects.","PeriodicalId":23706,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete","volume":"26 5","pages":"419-24"},"PeriodicalIF":0.0,"publicationDate":"1971-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znb-1971-0509","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15499572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The concentration dependence of the dielectric dispersion between 100 kHz and 15 MHz has been investigated for separated α and β chains of human hemoglobin. It is shown that such dielectric measurements can give very detailed information on aggregation phenomena. Specific dielectric increments and dipole moments of monomeric and aggregated chains could be obtained.
{"title":"Dielectric studies of aggregation phenomena with separated alpha and beta chains of human hemoglobin.","authors":"P Schlecht","doi":"10.1515/znb-1971-0514","DOIUrl":"https://doi.org/10.1515/znb-1971-0514","url":null,"abstract":"The concentration dependence of the dielectric dispersion between 100 kHz and 15 MHz has been investigated for separated α and β chains of human hemoglobin. It is shown that such dielectric measurements can give very detailed information on aggregation phenomena. Specific dielectric increments and dipole moments of monomeric and aggregated chains could be obtained.","PeriodicalId":23706,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete","volume":"26 5","pages":"453-7"},"PeriodicalIF":0.0,"publicationDate":"1971-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znb-1971-0514","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15499578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[A method for preparing radioactive labeled phosphatides using soybeans in vivo].","authors":"J Hölzl, H Wagner","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":23706,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete","volume":"26 5","pages":"425-34"},"PeriodicalIF":0.0,"publicationDate":"1971-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15499574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Preparation of derivatives of peptide antibiotics polymyxin B and colistin- substituted on free amino groups by aminoacyl residues is described. The first procedure is based on the condensation of the antibiotic with active ester of a suitably protected amino acid. The second one makes use of Woodward’s reagent by condensation of the antibiotic with a protected amino acid. The following compounds were prepared: glycyl-14C-polymyxin B and glutaminyl-polymyxin B and glycylcolistin, also under conditions suitable for a radioactive synthesis. All the preparations preserve a substantial part of the biological activity comparable with polymyxin B and colistin.
{"title":"A simple method of preparation of labelled peptides possessing antibiotic properties.","authors":"M Havránek, K Veres","doi":"10.1515/znb-1971-0513","DOIUrl":"https://doi.org/10.1515/znb-1971-0513","url":null,"abstract":"Preparation of derivatives of peptide antibiotics polymyxin B and colistin- substituted on free amino groups by aminoacyl residues is described. The first procedure is based on the condensation of the antibiotic with active ester of a suitably protected amino acid. The second one makes use of Woodward’s reagent by condensation of the antibiotic with a protected amino acid. The following compounds were prepared: glycyl-14C-polymyxin B and glutaminyl-polymyxin B and glycylcolistin, also under conditions suitable for a radioactive synthesis. All the preparations preserve a substantial part of the biological activity comparable with polymyxin B and colistin.","PeriodicalId":23706,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete","volume":"26 5","pages":"451-3"},"PeriodicalIF":0.0,"publicationDate":"1971-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znb-1971-0513","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15499577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Ultrastructure of the antennal gland of Polyxenus lagurus (L.) (Diplopoda)].","authors":"el-Hifnawi el-S","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":23706,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete","volume":"26 5","pages":"486-7"},"PeriodicalIF":0.0,"publicationDate":"1971-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15499581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[In vitro studies on the mode of action and differences to heparin. Anticomplementary effect of a semisynthetic pentosan-polysulfo-ester].","authors":"D Walb, M Loos, U Hadding","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":23706,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete","volume":"26 5","pages":"403-8"},"PeriodicalIF":0.0,"publicationDate":"1971-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15499570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A number of independent systems for sugar transport in Neurospora have been postulated. An approach to identification of specific components of the sorbose transport system in ungerminated conidia pretreated with sorbose is described here. The fact that sorbose stimulates sorbose-uptake without causing germination was exploited in differential labeling experiments. Sorbose treated and untreated conidia were incubated with (3H) -leucine and (14C) -leucine respectively, and then mixed extracts fractionated on CM-cellulose at pH 4.75. Changes in the 3H/14C-ratio indicated sorbose-induced or -repressed substances. In selecting labeling conditions, care was taken to avoid errors that might arise from any differences in the internal amino acid pool size. The investigation revealed a buffer-soluble component repressed by sorbose and a Triton-soluble component induced by sorbose. The buffer-soluble, sorbose repressible protein is discussed in connection with the phenomenon of sorbose toxicity in Neurospora. The Triton-soluble, sorbose stimulated component, probably of carotenoid nature, is considered as a possible constituent of the sorbose transport system investigated here.
{"title":"Cellular components specifically labeled during sorbose stimulation of sugar transport in neurospora.","authors":"W Klingmüller","doi":"10.1515/znb-1971-0507","DOIUrl":"https://doi.org/10.1515/znb-1971-0507","url":null,"abstract":"A number of independent systems for sugar transport in Neurospora have been postulated. An approach to identification of specific components of the sorbose transport system in ungerminated conidia pretreated with sorbose is described here. The fact that sorbose stimulates sorbose-uptake without causing germination was exploited in differential labeling experiments. Sorbose treated and untreated conidia were incubated with (3H) -leucine and (14C) -leucine respectively, and then mixed extracts fractionated on CM-cellulose at pH 4.75. Changes in the 3H/14C-ratio indicated sorbose-induced or -repressed substances. In selecting labeling conditions, care was taken to avoid errors that might arise from any differences in the internal amino acid pool size. The investigation revealed a buffer-soluble component repressed by sorbose and a Triton-soluble component induced by sorbose. The buffer-soluble, sorbose repressible protein is discussed in connection with the phenomenon of sorbose toxicity in Neurospora. The Triton-soluble, sorbose stimulated component, probably of carotenoid nature, is considered as a possible constituent of the sorbose transport system investigated here.","PeriodicalId":23706,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete","volume":"26 5","pages":"409-13"},"PeriodicalIF":0.0,"publicationDate":"1971-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znb-1971-0507","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15499571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rod outer segments of frogs (Rana esculenta) were isolated by shaking the retina in R i n g e r solution. Before spreading each drop was examined by light microscopy and checked for purity. The outer segments were then either spread on the surface of a phosphotungstic acid solution (1.8 . . . 2.0%) containing 0 . 0 5 . . . 0.1% bovine serum albumin (pH 7.3) or negatively stained as drop preparations; instead of bovine serum albumin a 0.07% glycerol solution was also used. In contrast to micrographs of ultrathin sections (e.g. F E R N Ä N D E Z M O R Ä N 3 ; N I L S S O N 5 ) and previous negative staining experiments (BLASIE et al.2) which reveal "dense granules", "globular substructures" or "particles ~ 4 0 Ä in diameter" we found fibrillar elements (Fäden) in the outer segments as already postulated before on the basis of freeze-etching experiments (ROSENKRANZ 6 ) . In surface-spread and drop preparations we observed fibrils of different structure and diameter as well as partially preserved membranes. In preparations exposed to surface spreading we found predominantly well-spread, smooth fibrils (da). Their diameter is 49 Ä < da ^ 58 Ä, see Fig. 1 *. This wide range is due to the fact that several observers in our department made a total of 100 measurements from the same electron micrograph. Some micrographs indicate that each da-fibril might in turn be composed of fibrillar substructures.
{"title":"The frog's rod outer segment observed by the negative staining technique.","authors":"M Hauser, J Rosenkranz","doi":"10.1515/znb-1971-0526","DOIUrl":"https://doi.org/10.1515/znb-1971-0526","url":null,"abstract":"Rod outer segments of frogs (Rana esculenta) were isolated by shaking the retina in R i n g e r solution. Before spreading each drop was examined by light microscopy and checked for purity. The outer segments were then either spread on the surface of a phosphotungstic acid solution (1.8 . . . 2.0%) containing 0 . 0 5 . . . 0.1% bovine serum albumin (pH 7.3) or negatively stained as drop preparations; instead of bovine serum albumin a 0.07% glycerol solution was also used. In contrast to micrographs of ultrathin sections (e.g. F E R N Ä N D E Z M O R Ä N 3 ; N I L S S O N 5 ) and previous negative staining experiments (BLASIE et al.2) which reveal \"dense granules\", \"globular substructures\" or \"particles ~ 4 0 Ä in diameter\" we found fibrillar elements (Fäden) in the outer segments as already postulated before on the basis of freeze-etching experiments (ROSENKRANZ 6 ) . In surface-spread and drop preparations we observed fibrils of different structure and diameter as well as partially preserved membranes. In preparations exposed to surface spreading we found predominantly well-spread, smooth fibrils (da). Their diameter is 49 Ä < da ^ 58 Ä, see Fig. 1 *. This wide range is due to the fact that several observers in our department made a total of 100 measurements from the same electron micrograph. Some micrographs indicate that each da-fibril might in turn be composed of fibrillar substructures.","PeriodicalId":23706,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete","volume":"26 5","pages":"487"},"PeriodicalIF":0.0,"publicationDate":"1971-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znb-1971-0526","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15218386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：