The homogeneity of purified NAD-glycohydrolase (EC 3.2.2.5) from Group C streptococci was demonstrated by analytical ultracentrifugation, disc electrophoresis and immunochemical analysis. Together with earlier characterization procedures the data show that the enzyme is homogenous in molecular seize, molecular form and electrophoretic charge. In addition, the enzyme has been shown to possess strong hemolytic activity which could be activated by thiol compounds. It was concluded from the analytical data that NAD-glycohydrolase- and Streptolysin-O-activities are catalytic functions of one protein molecule. Therefore the lysis of red blood cells by Streptolysin-O may be regarded as an enzymatic process catalyzed by an NAD-glycohydrolase.
{"title":"Identity of streptolysin-O and NAD-glycohydrolase (EC 3.2.2.5).","authors":"F J Fehrenbach","doi":"10.1515/znb-1971-1224","DOIUrl":"https://doi.org/10.1515/znb-1971-1224","url":null,"abstract":"The homogeneity of purified NAD-glycohydrolase (EC 3.2.2.5) from Group C streptococci was demonstrated by analytical ultracentrifugation, disc electrophoresis and immunochemical analysis. Together with earlier characterization procedures the data show that the enzyme is homogenous in molecular seize, molecular form and electrophoretic charge. In addition, the enzyme has been shown to possess strong hemolytic activity which could be activated by thiol compounds. It was concluded from the analytical data that NAD-glycohydrolase- and Streptolysin-O-activities are catalytic functions of one protein molecule. Therefore the lysis of red blood cells by Streptolysin-O may be regarded as an enzymatic process catalyzed by an NAD-glycohydrolase.","PeriodicalId":23706,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete","volume":"26 12","pages":"1336-8"},"PeriodicalIF":0.0,"publicationDate":"1971-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znb-1971-1224","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15503187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Miriquidic acid, a new depside from Lecidea lilienstroemii and Lecidea leucophaea].","authors":"S Huneck, K Schreiber, G Snatzke, H W Fehlhaber","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":23706,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete","volume":"26 12","pages":"1357-64"},"PeriodicalIF":0.0,"publicationDate":"1971-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15503190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The longitudinal proton relaxation time T1 of aqueous solutions of bovine ferrihemoglobin and carboxilated hemoglobin was measured in the Larmor frequency range 3·103 Hz to 1,2·108 Hz. The dispersion curves are explained by diamagnetic and electron paramagnetic relaxation mechanisms. An efficient direct dipolar coupling between the paramagnetic heme-groups and the water protons in ferrihemoglobin solutions can be excluded. The flat dispersion behaviour of the diamagnetic contribution suggests a broad distribution of molecular motions i. e. a certain flexibility of the hemoglobin molecule.
{"title":"Nuclear magnetic relaxation spectroscopy in solutions of bovine hemoglobin.","authors":"R Kimmich","doi":"10.1515/znb-1971-1117","DOIUrl":"https://doi.org/10.1515/znb-1971-1117","url":null,"abstract":"The longitudinal proton relaxation time T1 of aqueous solutions of bovine ferrihemoglobin and carboxilated hemoglobin was measured in the Larmor frequency range 3·103 Hz to 1,2·108 Hz. The dispersion curves are explained by diamagnetic and electron paramagnetic relaxation mechanisms. An efficient direct dipolar coupling between the paramagnetic heme-groups and the water protons in ferrihemoglobin solutions can be excluded. The flat dispersion behaviour of the diamagnetic contribution suggests a broad distribution of molecular motions i. e. a certain flexibility of the hemoglobin molecule.","PeriodicalId":23706,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete","volume":"266 11","pages":"1168-70"},"PeriodicalIF":0.0,"publicationDate":"1971-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znb-1971-1117","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15505381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Relationship between dose and effect].","authors":"A Sippel","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":23706,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete","volume":"266 11","pages":"1202-3"},"PeriodicalIF":0.0,"publicationDate":"1971-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15505386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Incorporation of intraduodenal by administered 14 C- 32 P-polyen-phosphatidyl choline into the liver and its secretion in the bile].","authors":"J Hölzl, H Wagner","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":23706,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete","volume":"266 11","pages":"1151-8"},"PeriodicalIF":0.0,"publicationDate":"1971-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15505379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"On the nature of the anti-dextran activity of the Helix pomatia \"anti A\" agglutinin.","authors":"I Ishiyama, G Uhlenbruck","doi":"10.1515/znb-1971-1128","DOIUrl":"https://doi.org/10.1515/znb-1971-1128","url":null,"abstract":"","PeriodicalId":23706,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete","volume":"266 11","pages":"1198-9"},"PeriodicalIF":0.0,"publicationDate":"1971-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znb-1971-1128","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15505383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[No effect of partial hepatectomy on the survival time of rats with Walker carcinosarcoma].","authors":"M Volm, K Wayss","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":23706,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete","volume":"266 11","pages":"1199-200"},"PeriodicalIF":0.0,"publicationDate":"1971-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15505384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Authors have studied the capacity to photoreact with native DNA and r-RNA using six coumarins and four 4′,5′-dihydro-furo-coumarins. Six of these substances have shown a definite photoreactivity both with DNA and r-RNA, even if in a very much lower extent in respect to that of skin-photosensitizing furocoumarins. The low capacity to photoreact with nucleic acids of these substances is discussed, also in connection with the absence of skin-photosensitizing activity.
{"title":"Photoreactivity (365 nm) of some coumarins and 4',5'-dihydro-furocoumarins with nucleic acids.","authors":"S Marciani, F Dall'Acqua, L Guelfi, D Vedaldi","doi":"10.1515/znb-1971-1111","DOIUrl":"https://doi.org/10.1515/znb-1971-1111","url":null,"abstract":"The Authors have studied the capacity to photoreact with native DNA and r-RNA using six coumarins and four 4′,5′-dihydro-furo-coumarins. Six of these substances have shown a definite photoreactivity both with DNA and r-RNA, even if in a very much lower extent in respect to that of skin-photosensitizing furocoumarins. The low capacity to photoreact with nucleic acids of these substances is discussed, also in connection with the absence of skin-photosensitizing activity.","PeriodicalId":23706,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete","volume":"266 11","pages":"1129-36"},"PeriodicalIF":0.0,"publicationDate":"1971-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znb-1971-1111","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15505378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Electrons produced by Chl-aII in a short flash are finally transfered to Chl-ai after having passed several intermediates. The reaction between Chl-aI and its primary electron donators PD (probably Cyt-f and PC) has been studied by means of high time resolution flash photometry in isolated spinach chloroplasts with the following results: 1. When PD are in the oxidized state, the reduction of Chl-aI⊕ takes place with the rate limiting reaction time of the electron transport chain (≈ 20 ms). 2. When PD are in the reduced state, the reduction of Chl-aI⊕ takes place with the reaction times between the PD and Chl-ai. We found two half life times: one of about 200 μs and one of about 10 μs. The reduced state of PD is realized when weak monitoring light or hvII-preillumination is used. The two reaction times of ≈ 200 μs and of ≈ 10 µs are interpreted to represent the electron transfers between Cyt-f and Chl-aI and between PC and Chl-aI resp. Equilibrium constants of the electron transfer from PD to Chl-aI and the arrangement of the intermediates in the electron transport chain are discussed.
{"title":"On the reaction between chlorophyll-a, and its primary electron donors in photosynthesis.","authors":"W Haehnel, G Döring, H T Witt","doi":"10.1515/znb-1971-1118","DOIUrl":"https://doi.org/10.1515/znb-1971-1118","url":null,"abstract":"Electrons produced by Chl-aII in a short flash are finally transfered to Chl-ai after having passed several intermediates. The reaction between Chl-aI and its primary electron donators PD (probably Cyt-f and PC) has been studied by means of high time resolution flash photometry in isolated spinach chloroplasts with the following results: 1. When PD are in the oxidized state, the reduction of Chl-aI⊕ takes place with the rate limiting reaction time of the electron transport chain (≈ 20 ms). 2. When PD are in the reduced state, the reduction of Chl-aI⊕ takes place with the reaction times between the PD and Chl-ai. We found two half life times: one of about 200 μs and one of about 10 μs. The reduced state of PD is realized when weak monitoring light or hvII-preillumination is used. The two reaction times of ≈ 200 μs and of ≈ 10 µs are interpreted to represent the electron transfers between Cyt-f and Chl-aI and between PC and Chl-aI resp. Equilibrium constants of the electron transfer from PD to Chl-aI and the arrangement of the intermediates in the electron transport chain are discussed.","PeriodicalId":23706,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete","volume":"266 11","pages":"1171-4"},"PeriodicalIF":0.0,"publicationDate":"1971-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znb-1971-1118","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15505382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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