Pub Date : 2012-11-01DOI: 10.3917/arsi.forma.2012.01.0180
M. Formarier
{"title":"Équipe, travail en équipe","authors":"M. Formarier","doi":"10.3917/arsi.forma.2012.01.0180","DOIUrl":"https://doi.org/10.3917/arsi.forma.2012.01.0180","url":null,"abstract":"","PeriodicalId":237893,"journal":{"name":"Les concepts en sciences infirmières","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2012-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130604741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-11-01DOI: 10.3917/arsi.forma.2012.01.0318
B. Guerrin
{"title":"Projet de vie","authors":"B. Guerrin","doi":"10.3917/arsi.forma.2012.01.0318","DOIUrl":"https://doi.org/10.3917/arsi.forma.2012.01.0318","url":null,"abstract":"","PeriodicalId":237893,"journal":{"name":"Les concepts en sciences infirmières","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2012-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123077368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-11-01DOI: 10.3917/arsi.forma.2012.01.0197
Maud-Christine Chouinard
{"title":"Gestion de cas","authors":"Maud-Christine Chouinard","doi":"10.3917/arsi.forma.2012.01.0197","DOIUrl":"https://doi.org/10.3917/arsi.forma.2012.01.0197","url":null,"abstract":"","PeriodicalId":237893,"journal":{"name":"Les concepts en sciences infirmières","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2012-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121346856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-11-01DOI: 10.3917/arsi.forma.2012.01.0213
D. Friard
Agarrwal, R., Padmakumari, A. P., Bentur, J. S., & Nair, S. (2016). Metabolic and transcriptomic changes induced in host during hypersensitive response mediated resistance in rice against the Asian rice gall midge. Rice, 9(1), 1‐15. https://doi.org/10.1186/s12284-016-0077-6 Background An incompatible interaction between rice (Oryza sativa) and the Asian rice gall midge (AGM, Orseolia oryzae Wood-Mason), that is usually manifested through a hypersensitive response (HR), represents an intricate relationship between the resistant host and its avirulent pest. We investigated changes in the transcriptome and metabolome of the host (indica rice variety: RP2068-18-3-5, RP), showing HR when attacked by an avirulent gall midge biotype (GMB1), to deduce molecular and biochemical bases of such a complex interaction. Till now, such an integrated analysis of host transcriptome and metabolome has not been reported for any rice-insect interaction. Results Transcript and metabolic profiling data revealed more than 7000 differentially expressed genes and 80 differentially accumulated metabolites, respectively, in the resistant host. Microarray data revealed deregulation of carbon (C) and nitrogen (N) metabolism causing a C/N shift; up-regulation of tetrapyrrole synthesis and down-regulation of chlorophyll synthesis and photosynthesis. Integrated results revealed that genes involved in lipid peroxidation (LPO) were up-regulated and a marker metabolite for LPO (azelaic acid) accumulated during HR. This coincided with a greater accumulation of GABA (neurotransmitter and an insect antifeedant) at the feeding site. Validation of microarray results by semi-quantitative RT-PCR revealed temporal variation in gene expression profiles. Conclusions The study revealed extensive reprogramming of the transcriptome and metabolome of RP upon GMB1 infestation leading to an HR that was induced by the generation and release of reactive oxygen species i.e. singlet oxygen and resulted in LPO-mediated cell death. RP thus used HR as a means to limit nutrient supply to the feeding maggots and simultaneously accumulated GABA, strategies that could have led to maggot mortality. The integrated results of transcript and metabolic profiling, for the first time, provided insights into an HR+ type of resistance in rice against gall midge.
Agarrwal, R.， Padmakumari, A. P.， Bentur, J. S.和Nair, S.(2016)。水稻抗瘿蚊过程中宿主代谢和转录组学的变化。水稻，9(1)，1‐15。https://doi.org/10.1186/s12284-016-0077-6背景水稻(Oryza sativa)与亚洲稻瘿蚊(Orseolia oryzae Wood-Mason)之间的不相容相互作用通常表现为超敏反应(hypersensitive response, HR)，这代表了抗性寄主与其无毒害虫之间的复杂关系。我们研究了宿主(籼稻品种:RP2068-18-3-5, RP)的转录组和代谢组的变化，显示了受到无害瘿蚊生物型(GMB1)攻击时的HR，以推断这种复杂相互作用的分子和生化基础。到目前为止，这样的寄主转录组和代谢组的综合分析还没有任何水稻-昆虫互作的报道。结果转录和代谢分析数据显示，抗性寄主中分别存在7000多个差异表达基因和80多个差异积累代谢物。微阵列数据显示碳(C)和氮(N)代谢失调导致碳/氮转移;上调四吡咯合成，下调叶绿素合成和光合作用。综合结果显示，与脂质过氧化(LPO)相关的基因在HR期间被上调，并且LPO(壬二酸)的标记代谢物在HR期间积累。这与进食部位GABA(神经递质和昆虫抗食性物质)的大量积累相吻合。通过半定量RT-PCR验证微阵列结果揭示了基因表达谱的时间变化。该研究揭示了GMB1侵染后RP转录组和代谢组的广泛重编程，导致由活性氧(即单重态氧)的产生和释放诱导的HR，并导致lpo介导的细胞死亡。因此，RP利用HR作为一种手段来限制进食的蛆虫的营养供应，同时积累GABA，这些策略可能导致蛆虫死亡。转录和代谢分析的综合结果首次为水稻对瘿蚊的HR+抗性提供了新的见解。
{"title":"Mécanismes de défense","authors":"D. Friard","doi":"10.3917/arsi.forma.2012.01.0213","DOIUrl":"https://doi.org/10.3917/arsi.forma.2012.01.0213","url":null,"abstract":"Agarrwal, R., Padmakumari, A. P., Bentur, J. S., & Nair, S. (2016). Metabolic and transcriptomic changes induced in host during hypersensitive response mediated resistance in rice against the Asian rice gall midge. Rice, 9(1), 1‐15. https://doi.org/10.1186/s12284-016-0077-6 Background An incompatible interaction between rice (Oryza sativa) and the Asian rice gall midge (AGM, Orseolia oryzae Wood-Mason), that is usually manifested through a hypersensitive response (HR), represents an intricate relationship between the resistant host and its avirulent pest. We investigated changes in the transcriptome and metabolome of the host (indica rice variety: RP2068-18-3-5, RP), showing HR when attacked by an avirulent gall midge biotype (GMB1), to deduce molecular and biochemical bases of such a complex interaction. Till now, such an integrated analysis of host transcriptome and metabolome has not been reported for any rice-insect interaction. Results Transcript and metabolic profiling data revealed more than 7000 differentially expressed genes and 80 differentially accumulated metabolites, respectively, in the resistant host. Microarray data revealed deregulation of carbon (C) and nitrogen (N) metabolism causing a C/N shift; up-regulation of tetrapyrrole synthesis and down-regulation of chlorophyll synthesis and photosynthesis. Integrated results revealed that genes involved in lipid peroxidation (LPO) were up-regulated and a marker metabolite for LPO (azelaic acid) accumulated during HR. This coincided with a greater accumulation of GABA (neurotransmitter and an insect antifeedant) at the feeding site. Validation of microarray results by semi-quantitative RT-PCR revealed temporal variation in gene expression profiles. Conclusions The study revealed extensive reprogramming of the transcriptome and metabolome of RP upon GMB1 infestation leading to an HR that was induced by the generation and release of reactive oxygen species i.e. singlet oxygen and resulted in LPO-mediated cell death. RP thus used HR as a means to limit nutrient supply to the feeding maggots and simultaneously accumulated GABA, strategies that could have led to maggot mortality. The integrated results of transcript and metabolic profiling, for the first time, provided insights into an HR+ type of resistance in rice against gall midge.","PeriodicalId":237893,"journal":{"name":"Les concepts en sciences infirmières","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2012-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134399429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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