Wiadomosci parazytologiczne最新文献

Myiasis is a rare, worldwide, human disease with seasonal variation, caused by developing larvae of a variety of fly species. It can be dangerous when infestations penetrate into the brain. In the available literature, we have found only a few papers concerning ear myiasis caused by Lucilia sericata. Here, we report 2 cases of aural myiasis. Early intervention (surgical removal, occlusion) in these cases should prevent complications. Larvae, for further examination, should be killed by immersion in very hot water, then preserved in an ethanol.

This paper reviews the results of an extensive monitoring study, spanning 20-years of observation from three medical centres in Olsztyn on the dynamics and species diversity of fungi most frequently colonizing the respiratory and digestive systems of humans. The experimental materials were swabs and specimens from the gastrointestinal tract, swabs from the oral cavity and pharynx, as well as sputum and bronchial fluid from the respiratory system. The biological material was subjected to routine mycological diagnostics, taxonomic determination and identification. In total, 41 species of yeast and yeast-like fungi were isolated, including 34 from the respiratory and 25 from the digestive system. In the last decade, a significant increase has been noticed in the counts of fungi, especially the gastrointestinal tract, reported from people. As many as 18 species were isolated from both systems--they were predominated by fungi of the genus Candida and their perfect forms. Worthy of notice are also frequent isolations of yeast (Saccharomyces spp.) and detection of an endemic species, Paracoccidioides brasiliensis, in the respiratory system, and of the sexual stages of Rhodosporidium diobovatum and Rhodosporidium kratochvilovae from the gastrointestinal tract.

Candida albicans is a major human fungal pathogen especially as an etiologic agent of opportunistic oral and genital infections. Moreover, C. albicans can be involved in the deep infections and recent evidence suggests that the majority of diseases produced by this pathogen are associated with biofilm growth. The aims of this study were to evaluate biofilm production ability of C. albicans strains isolated from different sources, and to evaluate the effect of serum for enhancement the growth of biofilm. The strains used in this study were obtained from three sources; 12 from feces of patients with gastrointestinal disturbances, 13 from the oral cavity of patients with oral candidiasis, and 16 from the vagina of patients with Candida vulvovaginitis (CVV). Polystyrene 96-well plates were used to grow biofilms and crystal violet (CV) staining method was used to evaluate the growth. There were no differences in biofilm growth expressed as CV absorbance between C. albicans strains from different origins neither in Yeast Nitrogen Base broth (YNB) or in bovine serum (BS) (ANOVA, P = 0.1648, P = 0.5106, respectively). In the BS, the biofilm production was greater than in YNB medium for all samples (ANOVA, P = 0.0003).

The genus Scopulariopsis is a common soil saprotroph and has been isolated from air, organic waste and also from plant, animal and human tissues. Scopulariopsis has mainly been associated in humans with superficial mycoses, but it has also been described as the cause of subcutaneous and invasive infections. The most common aetiological agent of infections in humans is Scopulariopsis brevicaulis. This species has been reported to be resistant in vitro to broad-spectrum antifungal agents available today. The aim of the study was to establish in vitro antifungal susceptibility of 35 S. brevicaulis strains against amphotericin B (AMB), flucytosine (FC), caspofungin (CAS), terbinafine (TER), ciclopirox (CIC), voriconazole (VOR), clotrimazole (CTR), miconazole (MCZ), econazole (ECO), ketoconazole (KET), itraconazole (ITR), and fluconazole (FLU). Antifungal susceptibility tests were evaluated by an agar diffusion method (Neo-Sensitabs, Rosco, Denmark). AMB, FC, CAS, ITR and FLU showed no antifungal activity against S. brevicaulis. TER, CIC, CTR, KET, VOR, ECO, and MCZ revealed inhibitory activity for S. brevicaulis, but it varied for each of the drugs. The best antifungal effect was observed for TER and CIC. All isolates had large inhibition zones for TER and CIC. CTR was also inhibitory for all tested S. brevicaulis isolates, but the diameters of inhibition zones were smaller than for TER and CIC. Nearly 89% isolates showed inhibition zones for KET and the mean diameter of the inhibition zone was comparable to CTR. The least antifungal activity exhibited VQR, ECO and MCZ. Because of the multiresistance of S. brevicaulis, infections due to this species may not respond to particular antifungal treatment and other therapeutic approaches should be considered, e.g., combined therapy and/or surgery.

Primary infection with Pneumocystis jirovecii in small children may cause inflammation of the respiratory tract which requires hospitalization. Lack of characteristic clinical symptoms makes it impossible to recognize P. jirovecii infections without performing laboratory analyses. Nasopharyngeal swabs from 70 children with respiratory tract infections were screened for fragments of the P. jirovecii genome. Pneumocystis DNA was found in swabs taken from two (2.9%) of the tested children: a newborn who was infected in the hospital and a six month old baby admitted to hospital two days after pneumonia was diagnosed. The obtained results confirm that primary P. jirovecii infections may occur in the form of acute respiratory tract inflammations suggesting a viral infection. In differential diagnosis of Pneumocystis infections in children molecular methods are useful as their high sensitivity makes it possible to analyze samples obtained in a non-invasive way.

Inter-individual variation in immune response to widely used prophylactic vaccines against infectious diseases is strongly influenced by sex, MHC (Major Histocompatibility Complex), age and current hormones status of vaccinated individuals. Numerous findings showed that microorganisms residing at different sites of human or animal body (natural microbiota), especially in the gastrointestinal tract, appear to contribute to nearly every element of the host's physiology. Recently, the microbiota is also supposed to be an underappreciated yet, but very important factor responsible for diverse vaccine efficacy observed in humans from developing vs. developed countries. In the article, selected aspects of the microbiota-host relation are presented: importance of the gut microbiota in the development of both the intestinal mucosal and systemic immune responses, bacteria of a predominant role for the immunity (e.g., SFB, Segmented Filamentous Bacteria), and several clinical observations on the varied immunogenicity of the same vaccines in different human populations. In the light of our current knowledge, manipulation of the microbiota by probiotics and/or prebiotics is becoming a realistic therapeutic and prophylactic strategy for many infectious, inflammatory and even neoplastic diseases within the gut but it may be also used for improving vaccine efficacy.

Babesiosis in humans is caused by infection with various species of Babesia (Apicomplexa, Piroplasmida), mainly transmitted by an arthropod vector--Ixodes spp. ticks. This review will focus on blood transfusion as another mode of Babesia transmission, especially in endemic areas, as well as the impact of human babesiosis on transfusion medicine.

Studies of fungi and fungus- like organisms in the northeastern Poland have mainly concentrated on running waters in the vicinity of Białystok, including the Horodnianka River. The main objective was to investigate biodiversity of fungi and fungus-like organisms which take part in decomposition of organic matter commonly found in inland waters. To obtain a complete picture of species composition of fungi and fungus-like organisms in running waters we decided to explore representative sites of the Horodnianka River such as Olmonty, Hryniewicze and Horodniany with close localization of landfill. Fungal species were isolated using baiting technique. Baits of onion skin (Alium cepa), hemp-seeds (Cannabis sativa), impregnated cellophane and snake skin (Natrix natrix) were applied to isolate fungi from water of the Horodnianka River. The fungal community consists of 26 species, 10 species of fungi belonging to class Chytridiomycetes (3), anamorphic fungi (6), and Zygomycetes (1). 16 species belong to fungus-like organisms from class Oomycetes. Most of the recognized species have already been found in other running waters. From all the examined habitats the fungi belonging to 26 species of 18 genera Achlya, Alternaria, Aphanomyces, Aspergillus, Catenophlyctis, Dictyuchus, Fusarium, Karlingia, Lagenidium, Leptomitus, Olpidiopsis, Penicillium, Phlyctochytrium, Pythium, Saprolegnia, Scoliognia, Thraustotheca and Zoophagus were obtained. Certain fungal species like Aphanomyces laevis, Fusarium aqueductum, F. moniliforme, F. oxysporum, Leptomitus lacteus, Saprolegnia feax and S. parasitica were found at all the study sites. Among fungi potentially pathogenic and allergogenic for humans the genera Alternaria, Aspergillus, Fusarium, Lagenidium and Penicillium have already been described. However, the species Lagenidium giganteum and Achlya androgyna are new in the fungal biota of Poland. The greatest number of fungal species occurred in Olmonty (24), the smallest in Horodniany (13). Presence of fungi such as Leptomitus lacteus, Fusarium aqueductum in the water of the Horodnianka River offers the possibility of using them as indicators of water quality.

Domestic dogs in Poland are attacked by five species of ticks. The present study shows results of research on species of ticks, their intensity and prevalence of infection in the domestic dogs in the district of Rymanów (Subcarpathian province, Poland). Local research was conducted from 25 August 2005 to 27 October 2006 on 82 randomly selected dogs in the veterinarian centre in Rymanów. Collected ticks were put into tubes with 70% ethyl alcohol. Collected and full up ticks were examined straight after being taken out of the preserving liquid. Stereoscopic microscope for species identification was used. The study revealed that domestic dogs in the Rymanów district are hosts for two species of ticks from the Ixodidae family: Ixodes (Ixodes) ricinus (Linnaeus, 1758) and Ixodes (Pholeoixodes) hexagonus Leach, 1815. Only one dog was infected with both tick species simultaneously. The dogs were attacked mainly by females. Ticks usually attached to such places as the neck, ear conches or groin. In general, the prevalence of ticks infestation on domestic dogs was 48.78%; I. ricinus prevalence was 47.56%, whereas I. hexagonus--2.43%. On the basis of obtained results it can be claimed that I. ricinus is more prevalent in the Rymanów district than I. hexagonus, therefore the domestic dogs are more frequently attacked by this species of ticks.

Information on species of parasitic helminths native to Poland is provided by the Pasozytnicze helminty Polski. Gatunki. Zywiciele. Białe Plamy. Until the middle of 2007, there were 1205 species of recorded helminths including 126 species of Monogenea, 338 of Trematoda (Digenea), 279 of Cestoda, 427 of Nematoda and 35 of Acanthocephala. They represented 32.9% of the species recorded in Europe (i.e., registered in the Fauna Europaea database). During the following three years up to the middle of 2010, 64 new species of Polish helminths were detected: 7 Monogenea, 15 Trematoda (Digenea), 21 Cestoda, 20 Nematoda and one Acanthocephala. Most hosts of the new helminth species were reported from birds (40 species), but also from fish (13 species) and mammals (10 species). Only one new species of helminth was detected in amphibians.