Łukasz Adaszek, Marta Górna, Michał Krzysiak, Michał Adaszek, Marcin Garbal, Stanisław Winiarczyk
The study was aimed at determining the cause of the diseases in three horses exhibiting symptoms of fever, ataxia, mucus membrane paleness, haematuria and thrombocytopenia. The PCR technique revealed the presence in the blood of 18S RNA Babesia/Theileria spp. genetic material. DNA amplification using primers RLB F2 and RLB R2 produced 430 bp size products. The sequences of these PCR products demonstrated a 95.6-97.5% similarity with the sequence of the fragment of 18S RNA Babesia equi, gene number DQ287951 in the GenBank. The treatment utilizing the subcutaneous application of the imidocarb resulted in gradual recovery of the diseased animals.
{"title":"Identification of the piroplasms isolated from horses with clinical piroplasmosis in Poland.","authors":"Łukasz Adaszek, Marta Górna, Michał Krzysiak, Michał Adaszek, Marcin Garbal, Stanisław Winiarczyk","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The study was aimed at determining the cause of the diseases in three horses exhibiting symptoms of fever, ataxia, mucus membrane paleness, haematuria and thrombocytopenia. The PCR technique revealed the presence in the blood of 18S RNA Babesia/Theileria spp. genetic material. DNA amplification using primers RLB F2 and RLB R2 produced 430 bp size products. The sequences of these PCR products demonstrated a 95.6-97.5% similarity with the sequence of the fragment of 18S RNA Babesia equi, gene number DQ287951 in the GenBank. The treatment utilizing the subcutaneous application of the imidocarb resulted in gradual recovery of the diseased animals.</p>","PeriodicalId":23835,"journal":{"name":"Wiadomosci parazytologiczne","volume":"57 1","pages":"21-6"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29908213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Małgorzata R Nowak, Katarzyna Królaczyk, Katarzyna M Kavetska, Bogumiła Pilarczyk
The tapeworm species Cloacotaenia megalops (Nitzsch in Creplin, 1829) is characterized by a very specific morphology. A particularly distinct feature is a large almost square scolex in which four fleshy suction cups are embedded and a rudimentary rostellum. During standard studies of wild duck cestode fauna in northwestern Poland, some morphological differences were observed among specimens, mainly in the shape of scolices and hermaphroditic proglottids of C. megalops isolated from different bird species. This paper attempts to demonstrate the impact of the host species on morphology of the parasite. The study material consisted of 39 individuals of C. megalops (19 from Aythya fuligula, 10 from Anas platyrhynchos, 4 from Bucephala clangula, 4 from Aythya marila and 2 from A. ferina). The isolated parasites were fixed and stored in 70% ethanol and solid preparations were made. Selected external and internal structures of the parasite were measured and photographed using a Zeiss microscope, a microscope camera Opta Tech 2.1 and Opta View 6.0.2.2. It was stated that the sizes of the most important elements of the parasite morphology are generally consistent with those presented in the available literature, nevertheless some differences were observed in both morphology and sizes of some anatomical structures, especially in the form of scolices and hermaphroditic proglottids between specimens isolated from hosts representing three different eco-tribes (Aythyini, Anatini and Mergini).
{"title":"Morphological features of Cloacotaenia megalops (Nitzsch in Creplin, 1829) (Cestoda, Hymenolepididae) from different hosts.","authors":"Małgorzata R Nowak, Katarzyna Królaczyk, Katarzyna M Kavetska, Bogumiła Pilarczyk","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The tapeworm species Cloacotaenia megalops (Nitzsch in Creplin, 1829) is characterized by a very specific morphology. A particularly distinct feature is a large almost square scolex in which four fleshy suction cups are embedded and a rudimentary rostellum. During standard studies of wild duck cestode fauna in northwestern Poland, some morphological differences were observed among specimens, mainly in the shape of scolices and hermaphroditic proglottids of C. megalops isolated from different bird species. This paper attempts to demonstrate the impact of the host species on morphology of the parasite. The study material consisted of 39 individuals of C. megalops (19 from Aythya fuligula, 10 from Anas platyrhynchos, 4 from Bucephala clangula, 4 from Aythya marila and 2 from A. ferina). The isolated parasites were fixed and stored in 70% ethanol and solid preparations were made. Selected external and internal structures of the parasite were measured and photographed using a Zeiss microscope, a microscope camera Opta Tech 2.1 and Opta View 6.0.2.2. It was stated that the sizes of the most important elements of the parasite morphology are generally consistent with those presented in the available literature, nevertheless some differences were observed in both morphology and sizes of some anatomical structures, especially in the form of scolices and hermaphroditic proglottids between specimens isolated from hosts representing three different eco-tribes (Aythyini, Anatini and Mergini).</p>","PeriodicalId":23835,"journal":{"name":"Wiadomosci parazytologiczne","volume":"57 1","pages":"31-6"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29908215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In July-August of 2005-2008, 47 digenean trematodes were examined from 7 specimens of the common sandpiper, Actitis hypoleucos (L., 1758). The digeneans were collected during a study on the food composition of the common sandpiper. The research was conducted near Lisewo Malborskie (near Tczew, Zuławy Wiślane, Gdańsk sea-coast), at a resting site on the birds' migration route. The foods ingested were extracted from live birds by draining their alimentary tracts with a nasogastric tube. Two digenean species were identified: Plagiorchis nanus Rudolphi, 1802 (Plagiorchiidae) and Leucochloridium perturbatum Pojmańska, 1969 (Leucochloridiidae). Actitis hypoleucos is the host for these new records in Poland. The P. nanus individuals isolated from A. hypoleucos differed in terms of the visible uterus and the extent of the vitellaria in the posterior part of the body.
{"title":"Actitis hypoleucos (L., 1758)--new Polish host of the trematodes: Plagiorchis nanus (Rudolphi, 1802) and Leucochloridium perturbatum Pojmańska, 1969.","authors":"Izabella Rzad, Ewa Dzika, Robert Krupa","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In July-August of 2005-2008, 47 digenean trematodes were examined from 7 specimens of the common sandpiper, Actitis hypoleucos (L., 1758). The digeneans were collected during a study on the food composition of the common sandpiper. The research was conducted near Lisewo Malborskie (near Tczew, Zuławy Wiślane, Gdańsk sea-coast), at a resting site on the birds' migration route. The foods ingested were extracted from live birds by draining their alimentary tracts with a nasogastric tube. Two digenean species were identified: Plagiorchis nanus Rudolphi, 1802 (Plagiorchiidae) and Leucochloridium perturbatum Pojmańska, 1969 (Leucochloridiidae). Actitis hypoleucos is the host for these new records in Poland. The P. nanus individuals isolated from A. hypoleucos differed in terms of the visible uterus and the extent of the vitellaria in the posterior part of the body.</p>","PeriodicalId":23835,"journal":{"name":"Wiadomosci parazytologiczne","volume":"57 1","pages":"37-41"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29908216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Protocol of the Polish Parasitology Society (PT)Congress on January 21, 2011].","authors":"Anna Rocka","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":23835,"journal":{"name":"Wiadomosci parazytologiczne","volume":"57 1","pages":"63-6"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29911590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Małgorzata Raś-Noryńska, Maria Michalczyk, Rajmund Sokół
Coccidiosis caused by Eimeria spp. is a common parasitic disease in homing pigeons. The study objective was to evaluate the incidence of coccidia infections in pigeon lofts during racing season. The intensity of coccidiosis was determined by floatation analyses of faeces samples collected from three pigeon groups performed in three replications (before the racing season, in mid-season and after the end of racing season). The presence of coccidia oocysts was determined in all faeces samples in each replication. At the end of the racing season, the average oocyst counts in faeces samples collected from pigeons that were flown for the first time increased by around 10% in relation to oocysts counts determined before the race. In flown pigeons (aged 2-4 years) a 2.5-9.9% drop was noted in oocysts counts subject to flock, whereas an increase of 15.7-17.3% was reported in parent flocks (unflown pigeons). The results of the experiment indicate that coccidia infections are a common problem in homing pigeons during racing season, which affects racing results and contributes to bird loss.
{"title":"Coccidia infections in homing pigeons of various age during the racing season.","authors":"Małgorzata Raś-Noryńska, Maria Michalczyk, Rajmund Sokół","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Coccidiosis caused by Eimeria spp. is a common parasitic disease in homing pigeons. The study objective was to evaluate the incidence of coccidia infections in pigeon lofts during racing season. The intensity of coccidiosis was determined by floatation analyses of faeces samples collected from three pigeon groups performed in three replications (before the racing season, in mid-season and after the end of racing season). The presence of coccidia oocysts was determined in all faeces samples in each replication. At the end of the racing season, the average oocyst counts in faeces samples collected from pigeons that were flown for the first time increased by around 10% in relation to oocysts counts determined before the race. In flown pigeons (aged 2-4 years) a 2.5-9.9% drop was noted in oocysts counts subject to flock, whereas an increase of 15.7-17.3% was reported in parent flocks (unflown pigeons). The results of the experiment indicate that coccidia infections are a common problem in homing pigeons during racing season, which affects racing results and contributes to bird loss.</p>","PeriodicalId":23835,"journal":{"name":"Wiadomosci parazytologiczne","volume":"57 3","pages":"165-8"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30322290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Between 2005 and 2008 cysticercosis caused by Cysticercus bovis was detected in 1485 (0.44%) animals out of 335 436 heads of cattle slaughtered in the Lublin province. This particular cysticercosis was observed each year. In the period 2005-2008 cysticercosis caused by Cysticercus cellulosae was found only in a few pigs (93 cases--0.0059% out of 1 577 370 examined pigs).
{"title":"Prevalence of cysticercosis in cattle and pigs in the Lublin province in the years 2005-2008.","authors":"Jolanta Kozłowska-Łój","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Between 2005 and 2008 cysticercosis caused by Cysticercus bovis was detected in 1485 (0.44%) animals out of 335 436 heads of cattle slaughtered in the Lublin province. This particular cysticercosis was observed each year. In the period 2005-2008 cysticercosis caused by Cysticercus cellulosae was found only in a few pigs (93 cases--0.0059% out of 1 577 370 examined pigs).</p>","PeriodicalId":23835,"journal":{"name":"Wiadomosci parazytologiczne","volume":"57 3","pages":"193-4"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30322295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dariusz Kaczmarczyk, Alina Morawiec-Sztandera, Izabela Niedźwiecka, Piotr Kurnatowski
Cases of fungal infections are being encountered more often in clinical practice. The factors associated with a high risk of mycoses include, among others, corticosteroidotherapy, the administration antibiotics with wide spectrum of antibacterial properties, neutropenia, neoplasms. Fungi may play a role in cancer formation, may act as a complication in the course of treatment, and may mimic a neoplastic process by giving a similar clinical picture. In the case of fungal throat infection, patients complain of increased body temperature, a general feeling of weakness, malaise, headache, spontaneous pain intensifying during swallowing, a feeling of an obstacle in the throat or a cough. A physical examination may reveal congestion of the mucosa followed by a unilateral crater ulceration often covered with fat, as well as a thick coating, which is accompanied by foetor ex ore. The submandibular and neck lymph nodes are often greatly enlarged and painful. These symptoms may resemble those associated with the neoplastic process and changes in the course of systemic diseases (agranulocytosis). A correct diagnosis in these cases is necessary for adequate therapy. Chronic lymphocytic leukemia (CLL) is the most common type of leukemia among adults in Europe and North America. It is estimated that in Poland, CLL affects approximately 1,400 people per year. In this paper, a case of 62-years old patient with CLL with fungal infection of oral cavity and throat is presented.
{"title":"Difficulties associated with the diagnosis of mycosis of the oral cavity and throat in chronic lymphocytic leukemia (CLL).","authors":"Dariusz Kaczmarczyk, Alina Morawiec-Sztandera, Izabela Niedźwiecka, Piotr Kurnatowski","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cases of fungal infections are being encountered more often in clinical practice. The factors associated with a high risk of mycoses include, among others, corticosteroidotherapy, the administration antibiotics with wide spectrum of antibacterial properties, neutropenia, neoplasms. Fungi may play a role in cancer formation, may act as a complication in the course of treatment, and may mimic a neoplastic process by giving a similar clinical picture. In the case of fungal throat infection, patients complain of increased body temperature, a general feeling of weakness, malaise, headache, spontaneous pain intensifying during swallowing, a feeling of an obstacle in the throat or a cough. A physical examination may reveal congestion of the mucosa followed by a unilateral crater ulceration often covered with fat, as well as a thick coating, which is accompanied by foetor ex ore. The submandibular and neck lymph nodes are often greatly enlarged and painful. These symptoms may resemble those associated with the neoplastic process and changes in the course of systemic diseases (agranulocytosis). A correct diagnosis in these cases is necessary for adequate therapy. Chronic lymphocytic leukemia (CLL) is the most common type of leukemia among adults in Europe and North America. It is estimated that in Poland, CLL affects approximately 1,400 people per year. In this paper, a case of 62-years old patient with CLL with fungal infection of oral cavity and throat is presented.</p>","PeriodicalId":23835,"journal":{"name":"Wiadomosci parazytologiczne","volume":"57 3","pages":"155-8"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30322343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aleksander Bielecki, Joanna M Cichocka, Iwona Jeleń, Piotr Swiatek, Zaneta Adamiak-Brud
In this study 47 leech species from Poland are listed. They belong to two orders, two suborders, five families and 17 genera. The checklist also includes the information about hosts, distribution in Poland and references concerning the leech species discussed in this study.
{"title":"A checklist of leech species from Poland.","authors":"Aleksander Bielecki, Joanna M Cichocka, Iwona Jeleń, Piotr Swiatek, Zaneta Adamiak-Brud","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In this study 47 leech species from Poland are listed. They belong to two orders, two suborders, five families and 17 genera. The checklist also includes the information about hosts, distribution in Poland and references concerning the leech species discussed in this study.</p>","PeriodicalId":23835,"journal":{"name":"Wiadomosci parazytologiczne","volume":"57 1","pages":"11-20"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29909315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Prevalence of Fasciola hepatica L. infection among cattle slaughtered in the Lublin province in the years 2005-2008 was evaluated. The study draws its data from annual reports of the Veterinary Inspectorate in Lublin. The results of the study are as follows: the prevalence of Fasciola hepatica infection in the cattle of the Lublin province between 2005 and 2008 amounts to 21.24%.
{"title":"Prevalence of Fasciola hepatica L. infection in cattle in the Lublin province (Poland) in the years 2005-2008.","authors":"Jolanta Kozłowska-Łój","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Prevalence of Fasciola hepatica L. infection among cattle slaughtered in the Lublin province in the years 2005-2008 was evaluated. The study draws its data from annual reports of the Veterinary Inspectorate in Lublin. The results of the study are as follows: the prevalence of Fasciola hepatica infection in the cattle of the Lublin province between 2005 and 2008 amounts to 21.24%.</p>","PeriodicalId":23835,"journal":{"name":"Wiadomosci parazytologiczne","volume":"57 2","pages":"127-8"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29944398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Infections related to modern surgical procedures present a difficult problem for contemporary medicine. Infections acquired during surgery represent a risk factor related to therapeutical interventions. Eradication of microorganisms from hospital operating theatre environment may contribute to reduction of infections as the laminar flow air-conditioning considerably reduces the number of microorganisms in the hospital environment. The objective of the study was to evaluate the occurrence of fungi in air-conditioned operating theatre rooms. The study was carried out in one of the hospitals in Krak6w during December 2009. Indoor air samples and imprints from the walls were collected from five operating theatre rooms. A total of fifty indoor air samples were collected with a MAS-100 device, and twenty five imprints from the walls were collected using a Count Tact method. Fungal growth was observed in 48 air samples; the average numbers of fungi were within the range of 5-100 c.f.u. in one cubic metre of the air. Fungi were detected only in four samples of the wall imprints; the number of fungi was 0.01 c.f.u. per one square centimetre of the surface. The mould genus Aspergillus was most frequently isolated, and the species A. fumigatus and A. versicolor were the dominating ones. To ensure microbiological cleanness of hospital operating theatre, the air-conditioning system should be properly maintained. Domination of the Aspergillus fungi in indoor air as well as increase in the number of moulds in the samples taken in evenings (p < 0.05) may suggest that the room decontamination procedures were neglected.
{"title":"Air-conditioning vs. presence of pathogenic fungi in hospital operating theatre environment.","authors":"Agnieszka Gniadek, Anna B Macura","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Infections related to modern surgical procedures present a difficult problem for contemporary medicine. Infections acquired during surgery represent a risk factor related to therapeutical interventions. Eradication of microorganisms from hospital operating theatre environment may contribute to reduction of infections as the laminar flow air-conditioning considerably reduces the number of microorganisms in the hospital environment. The objective of the study was to evaluate the occurrence of fungi in air-conditioned operating theatre rooms. The study was carried out in one of the hospitals in Krak6w during December 2009. Indoor air samples and imprints from the walls were collected from five operating theatre rooms. A total of fifty indoor air samples were collected with a MAS-100 device, and twenty five imprints from the walls were collected using a Count Tact method. Fungal growth was observed in 48 air samples; the average numbers of fungi were within the range of 5-100 c.f.u. in one cubic metre of the air. Fungi were detected only in four samples of the wall imprints; the number of fungi was 0.01 c.f.u. per one square centimetre of the surface. The mould genus Aspergillus was most frequently isolated, and the species A. fumigatus and A. versicolor were the dominating ones. To ensure microbiological cleanness of hospital operating theatre, the air-conditioning system should be properly maintained. Domination of the Aspergillus fungi in indoor air as well as increase in the number of moulds in the samples taken in evenings (p < 0.05) may suggest that the room decontamination procedures were neglected.</p>","PeriodicalId":23835,"journal":{"name":"Wiadomosci parazytologiczne","volume":"57 2","pages":"103-6"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29943976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}