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[pH-induced inactivation of glycerol dehydrogenase from Bacillus megaterium]. [ph诱导的巨芽孢杆菌甘油脱氢酶失活]。
Pub Date : 1984-06-01 DOI: 10.1515/znc-1984-0611
A Ganzhorn, M Scharschmidt, G Pfleiderer

The kinetic of the reversible dissociation of glycerol dehydrogenase from Bacillus megaterium in slightly alkaline medium was measured by biochemical, chemical and physical methods. The dissociation is followed by changes in the secondary structure and can be prevented by addition of NAD or increased potassium chloride concentration. Crosslinking by suberimidate, but not by monofunctional imido esters, shows a high stabilization against alkali, urea or heat inactivation caused by hindrance of dissociation.

采用生化、化学和物理等方法,研究了微碱性培养基中巨型芽孢杆菌甘油脱氢酶的可逆解离动力学。解离后二级结构发生变化,可以通过添加NAD或增加氯化钾浓度来防止。亚酰亚咪酯的交联,而不是单功能亚胺酯的交联,对碱、尿素或由解离障碍引起的热失活具有很高的稳定性。
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引用次数: 0
Packing pattern of DNA in bacteriophage T2. 噬菌体T2中DNA的包装模式。
Pub Date : 1984-06-01 DOI: 10.1515/znc-1984-0630
A N Ghosh, A Sen, N N Das Gupta

Osmotic shock was applied to phage T2 in such a manner that the compact-mass of DNA was released from phage. The shape of this compact-mass of DNA was studied under electron microscope. It appeared that the DNA was packed into an elongated icosahedron similar to the phage head.

渗透冲击作用于T2噬菌体,使致密的DNA从噬菌体中释放出来。在电子显微镜下研究了这种致密的DNA的形状。DNA似乎被包装成一个细长的二十面体,类似于噬菌体头。
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引用次数: 1
Thermal diffusion as a mechanism for biological transport. 热扩散作为一种生物运输机制。
Pub Date : 1984-06-01 DOI: 10.1515/znc-1984-0623
F J Bonner, L O Sundelöf

Accumulated experimental information is used to assess the possible significance of thermal diffusion to mass transport in living matter. Possible thermal gradients across membranes, a single living cell, and an ensemble of such cells (e.g. an organ, tumor, etc.) are estimated. The corresponding model calculations, although not describing the biological process in detail, lead to conclusions about the possibilities for thermal diffusion as follows. Adequate thermal gradients to support substantial thermal diffusion could exist across biological membranes. Thermal diffusive flow would become significant when ordinary Fickian diffusion is sufficiently suppressed, e.g. in more concentrated systems near critical points of solution (i.e. near incipient phase separations). Conditions favorable to thermal diffusion functioning as a mechanism for active transport appear possible. Thermal diffusion appears much more important for transport into and out of an ensemble of cells than into or out of a single cell. Such mass transport by thermal diffusion could assume a sizable magnitude for an ensemble of cells with the dimensions of an organ or a tumor.

积累的实验信息被用来评估热扩散对生物物质中质量输运的可能意义。估计跨膜、单个活细胞和这些细胞的集合(如器官、肿瘤等)可能的热梯度。相应的模型计算虽然没有详细描述生物过程,但得出了热扩散可能性的结论如下。足够的热梯度支持大量的热扩散可能存在于生物膜之间。当普通的菲克式扩散得到充分抑制时,例如在溶液临界点附近(即初相分离附近)更浓的体系中,热扩散流动将变得显著。有利于热扩散作为主动输送机制的条件似乎是可能的。热扩散对于进出细胞群的运输似乎比进出单个细胞更为重要。这种通过热扩散进行的质量传递,对于一个器官或肿瘤大小的细胞群来说,可能具有相当大的规模。
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引用次数: 25
Alteration of acylphosphate formation of cardiac sarcoplasmic reticulum ATPase by calmodulin-dependent phosphorylation. 钙调素依赖性磷酸化对心肌肌浆网atp酶酰基形成的影响。
Pub Date : 1984-03-01 DOI: 10.1515/znc-1984-3-415
C Pifl, B Plank, G Hellmann, W Wyskovsky, J Suko

The calcium-dependent acylphosphate formed by the calcium transport ATPase of cardiac sarcoplasmic reticulum and the calcium-, calmodulin-dependent phosphoester(s) of sarcoplasmic reticulum fractions formed by a calcium-, calmodulin-dependent membrane-bound protein kinase can be distinguished by removal of calcium and/or magnesium by EDTA or hydroxylamine treatment of the acid denaturated membranes. Both procedures decompose the acylphosphate with little effect on the phosphoester(s). Calmodulin-dependent phosphorylation (2.44 nmol/mg SR protein) reduces the apparent K(Ca) of the acylphosphate steady state level of the calcium transport ATPase from 0.56 to 0.34 microM free calcium, without affecting the maximum phosphoenzyme level (0.93 versus 0.89 nmol/mg protein), and has little, if any, effect on the Hill-coefficient (1.32 versus 1.54).

由心肌肌浆网钙转运atp酶形成的钙依赖性酰基磷酸和由钙、钙调素依赖的膜结合蛋白激酶形成的肌浆网部分的钙、钙调素依赖的磷酸酯可以通过EDTA或羟胺处理酸变性膜去除钙和/或镁来区分。这两种方法都能分解酰基磷酸,对磷酸酯几乎没有影响。钙调素依赖性磷酸化(2.44 nmol/mg SR蛋白)降低了钙转运atp酶的酰基磷酸盐稳态水平的表观K(Ca),从0.56 μ m游离钙降至0.34 μ m游离钙,而不影响最大磷酸化酶水平(0.93对0.89 nmol/mg蛋白),并且对hill系数(1.32对1.54)几乎没有影响。
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引用次数: 1
Isolation and characterization of tributyltin resistant mutants of Escherichia coli. 大肠杆菌耐三丁基锡突变体的分离与鉴定。
Pub Date : 1984-03-01 DOI: 10.1515/znc-1984-3-416
A P Singh, K Singh

Two classes of tributyltin (TBT) resistant, spontaneous mutants of Escherichia coli K-12 were isolated, using a cytochrome containing (W 1485) and a cytochrome deficient ( SASX76 ) strain. In contrast to the cytochrome sufficient strain, the cytochrome deficient strain was found to be fifty times more sensitive to TBT. The class I mutants, isolated from strain W 1485, also showed cross-resistance to triphenyltin (TPT). As compared to its wild type parent, the TBT-resistant mutants exhibited mucoid colony type, aberrant cell morphology and reduced uptake of TPT. Based on these results, it was suggested that the resistance of class I mutants to TBT may be associated with above mentioned alterations. The class II TBT-resistant mutants were isolated from the cytochrome deficient strain, SASX76 . In comparison to class I mutants, these class II mutants were found to have TBT-resistant membrane bound adenosine triphosphatase (ATPase) which may account for their resistance to TBT.

利用含有细胞色素(w1485)和缺乏细胞色素(SASX76)的菌株分离出两类耐三丁基锡(TBT)的大肠杆菌K-12自发突变体。与细胞色素充足菌株相比,细胞色素缺乏菌株对TBT的敏感性提高了50倍。从菌株w1485中分离出的I类突变体也表现出对三苯基锡(TPT)的交叉抗性。与野生型亲本相比,TPT抗性突变体表现为黏液集落型,细胞形态异常,TPT摄取减少。基于这些结果,我们认为I类突变体对TBT的抗性可能与上述改变有关。从细胞色素缺陷菌株SASX76中分离到II类tbt抗性突变体。与I类突变体相比,这些II类突变体被发现具有TBT抗性膜结合腺苷三磷酸酶(ATPase),这可能解释了它们对TBT的抗性。
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引用次数: 5
Aurintricarboxylic acid and polynucleotides as novel inhibitors of ribonucleotide reductases. 金羧酸和多核苷酸作为核糖核苷酸还原酶的新型抑制剂。
Pub Date : 1984-03-01 DOI: 10.1515/znc-1984-3-413
H Baumann, R Hofmann, M Lammers, G Schimpff-Weiland, H Follmann

Ribonucleoside diphosphate reductases isolated from Escherichia coli, baker's yeast, Ehrlich ascites tumor cells, and unicellular green alga (Scenedesmus obliquus) are inhibited strongly and uniformly by the polymeric triphenylmethane dye, aurintricarboxylic acid. The molecule appears to interact simultaneously with the enzyme's various nucleotide and catalytic (iron-organic radical) sites. Oligo- and polyribonucleotides are also inhibitory. These reactions serve as models of the probably physiologic regulation of ribonucleotide reduction exerted by natural inhibitors. Partial characterization of an inhibitor fraction found in wheat seed embryo is described.

从大肠杆菌、面包酵母、埃利希腹水肿瘤细胞和单细胞绿藻(斜绿藻)中分离出的核糖核苷二磷酸还原酶被聚合三苯基甲烷染料金羧酸强烈而均匀地抑制。该分子似乎同时与酶的各种核苷酸和催化(铁有机自由基)位点相互作用。寡核苷酸和多核糖核苷酸也具有抑制作用。这些反应可以作为天然抑制剂对核糖核苷酸还原可能的生理调节的模型。描述了小麦种子胚中发现的抑制剂组分的部分特性。
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引用次数: 4
Photoinactivation of Propionibacterium acnes by near-ultraviolet light. 近紫外光对痤疮丙酸杆菌的光灭活作用。
Pub Date : 1984-03-01 DOI: 10.1515/znc-1984-3-417
B Kjeldstad

Photodestruction of Propionibacterium acnes was investigated by broad-band near-ultraviolet light. The inactivation of the bacteria was found to be oxygen dependent, and without O2 practically no photoinactivation occurred. D2O caused an increased inactivation (D10 = 5 kJ/m2 in D2O as compared to D10 = 11 kJ/m2 in normal water). Decreased temperature during illumination increased the ability to form colonies. The results are compared with corresponding results for other types of cells and the destruction mechanism is discussed.

采用宽波段近紫外光对痤疮丙酸杆菌进行了光破坏研究。发现细菌的失活是氧气依赖的,没有氧气几乎没有光失活发生。D2O增加了失活(D2O中的D10 = 5 kJ/m2,而正常水中的D10 = 11 kJ/m2)。光照期间温度的降低增加了形成菌落的能力。结果与其他类型细胞的相应结果进行了比较,并讨论了其破坏机制。
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引用次数: 32
Activation volumes of the calcium dependent para-nitrophenyl phosphate hydrolysis of the sarcoplasmic reticulum calcium transport enzyme. 钙依赖性对硝基苯基磷酸水解肌浆网钙转运酶的活化体积。
Pub Date : 1984-03-01 DOI: 10.1515/znc-1984-3-414
K G König, W Hasselbach

The effect of pressure on the calcium dependent hydrolysis of para-nitrophenyl phosphate by the calcium transport enzyme of the sarcoplasmic reticulum was studied under different conditions: temperature, solutes, substrate and ion concentrations. The calcium transport enzyme exhibits a large positive activation volume which does neither depend on the enzyme's inhibition by high salt concentrations nor its activation by ethylene glycol. The activation volume further proves to be pressure-independent but exhibits a pronounced negative temperature coefficient. The volume changes connected with the entrance of para-nitrophenyl phosphate, calcium or magnesium ions into the substrate ion complex are quite small, indicating that the transfer of water connected with the binding of these ligands is compensated by volume changes of the protein, accompanying the transition of the enzyme from its activated into its ground state.

在温度、溶质、底物和离子浓度等不同条件下,研究了压力对肌浆网钙转运酶钙依赖性水解对硝基苯基磷酸的影响。钙转运酶表现出较大的正激活体积,这既不依赖于高盐浓度对酶的抑制,也不依赖于乙二醇对酶的激活。活化体积进一步证明与压力无关,但表现出明显的负温度系数。对硝基苯基磷酸盐、钙离子或镁离子进入底物离子络合物所引起的体积变化非常小,这表明与这些配体结合有关的水的转移被蛋白质的体积变化所补偿,伴随着酶从活化状态向基态的转变。
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引用次数: 6
Identification of a major pathway of strand break formation in poly U induced by OH radicals in presence of oxygen. 在氧存在下OH自由基诱导的聚U链断裂形成的主要途径的鉴定。
Pub Date : 1984-03-01 DOI: 10.1515/znc-1984-3-423
D Schulte-Frohlinde, E Bothe

A reaction mechanism is presented for strand break formation in poly U induced by OH radicals in N2O/O2- saturated aqueous solution based on experimental results obtained with different methods.

根据不同方法的实验结果,提出了在N2O/O2-饱和水溶液中OH自由基诱导聚U断链的反应机理。
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引用次数: 47
Insulin binding sites induced in the Tetrahymena by rat liver receptor antibody. 大鼠肝受体抗体诱导四膜虫胰岛素结合位点。
Pub Date : 1984-01-01 DOI: 10.1515/znc-1984-1-232
G Csaba, P Kovács, A Inczefi-Gonda

Tetrahymena cells treated with purified rabbit antibodies to rat hepatocellular membrane exhibited a considerable increase in binding capacity on reexposure to the antibody 24 h later. Insulin binding was similarly enhanced by preexposure to the antibody, and vice versa, preexposure to insulin enhanced the later binding of rat liver receptor antibodies. This suggests that (1) the Tetrahymena and the rat possess similar insulin receptors, and (2) the receptor antibody is also able to induce imprinting for itself as well as for insulin. Concanavalin-A, noted for binding overlap with insulin, failed to induce imprinting either for insulin or for antibodies to receptors, whereas the latter did induce imprinting for Concanavalin-A.

用纯化兔抗体处理大鼠肝细胞膜的四膜虫细胞在24小时后再次暴露于抗体时显示出相当大的结合能力。胰岛素结合同样通过预先暴露于抗体增强,反之亦然,预先暴露于胰岛素增强了大鼠肝脏受体抗体的后期结合。这表明:(1)四膜虫和大鼠具有相似的胰岛素受体,(2)受体抗体也能诱导自身和胰岛素的印记。刀豆蛋白a,以与胰岛素结合重叠而闻名,不能诱导胰岛素或受体抗体的印记,而后者却能诱导刀豆蛋白a的印记。
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引用次数: 12
期刊
Zeitschrift fur Naturforschung. Section C, Biosciences
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