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ANTIBIOTIC RESISTANCE PROFILE OF THE NEWLY ISOLATED LACTIC ACID BACTERIA STRAINS FROM TRADITIONAL FERMENTED FOODS 传统发酵食品中新分离乳酸菌的耐药性分析
Pub Date : 2022-07-31 DOI: 10.47068/ctns.2022.v11i21.027
Ramiz Hoxha, D. Nikolova, Y. Evstatieva
The use of antibiotics is a major problem everywhere around the globe and in step with this, there's currently an increased public and scientific interest. Microbial resistance is a vital issue for the organizations like EFSA, WHO, FDA, and FAO because it is developing rapidly and is an increasingly serious health concern within the world. Microbial resistance comes as a result of continuous exposure of microorganisms to antibiotics. Lactic acid bacteria (LAB) constitute a really important group of microorganisms that can inhabit different conditions and environments, as the different parts of the gastrointestinal tract of humans and animals, and are an important element of the microbiota of fermented foods. The FDA and EFSA authorities have given them the status referred to as GRAS (Generally Recognized as Safe) and QPS (Qualified Presumption of Safety). Continuous exposure of LAB to environments with antibiotics may prompt them to become an intrinsic or extrinsic reservoir of genes accountable for antibiotic resistance. Knowledge of antibiotic susceptibility of bacteria with probiotic potential is important. Also, it's essential to define their resistance profile. Ten newly isolated strains from traditional fermented foods were used for determining the antibiotic resistance profile. The resistance to antibiotics varied among the examined strains and a few of the antibiotics resulted in complete resistance. Chromosomal DNA of LAB was analyzed for the antibiotic resistance genes. Only five of eight vancomycin-resistant strains have shown that they contain the resistance gene in chromosomal DNA. None of the genes that determined the resistance to other antibiotics have been detected in chromosomal DNA.
抗生素的使用在全球各地都是一个大问题,与此同时,公众和科学界的兴趣也在增加。微生物耐药性对欧洲食品安全局、世卫组织、FDA和粮农组织等组织来说是一个至关重要的问题,因为它正在迅速发展,并在世界范围内成为一个日益严重的健康问题。微生物耐药性是微生物持续暴露于抗生素的结果。乳酸菌是一种非常重要的微生物群,作为人类和动物胃肠道的不同部位,可以生活在不同的条件和环境中,是发酵食品微生物群的重要组成部分。FDA和EFSA当局已经给予它们GRAS(一般公认安全)和QPS(安全合格推定)的地位。乳酸菌持续暴露于抗生素环境中,可能促使它们成为导致抗生素耐药性的内在或外在基因储存库。了解具有益生菌潜力的细菌的抗生素敏感性是重要的。此外,确定它们的阻力分布也很重要。采用从传统发酵食品中分离到的10株新菌株进行抗生素耐药谱测定。所检菌株对抗生素的耐药性各不相同,少数抗生素导致完全耐药。对乳酸菌染色体DNA进行耐药基因分析。在8种万古霉素耐药菌株中,只有5种显示它们在染色体DNA中含有耐药基因。在染色体DNA中没有检测到决定对其他抗生素耐药的基因。
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引用次数: 1
CROP PERFORMANCES AND GENETIC VARIABILITY ANALYSIS OF SEVEN TOMATO GENOTYPES CONDUCTED UNDER AGRO ECOLOGICAL FARMING MANAGEMENT 农业生态耕作管理下7个番茄基因型作物生产性能及遗传变异分析
Pub Date : 2022-07-31 DOI: 10.47068/ctns.2022.v11i21.043
Abdelmadjid Boulgheb, Zeineb Baaziz, Amina Falfali, L. Reguieg, A. Aissat
Tomato crop occupies a predominant place after that of potatoes in market garden crops in Algeria. The main purposes of this study were to evaluate crop performances, variability of some morphologic and phenologic traits, and correlations with the yield of seven (07) genotypes of tomato. The trials were carried out on Random Complete Blocs Design (RCBD) with three replications at the local unheated greenhouse during the period from November to June 2018. The most important morphological markers used were stem growth rate, inflorescence (Number of flowers, length of peduncle) and fruit characters (length and circumstance), phenologic stages, and average yield. The effect of the genetic material on the observed variability was significant for all the traits considered. Additionally, we noticed positive values of correlation coefficients of yield with stem length between every two clusters and the number of leave under cluster, average fruit weight, and earliness to flowering. Additionally, earliness to flowering shows very high and stable correlations (above 0.8) with yield during the crop cycle. The principal component analysis showed that the first two components generated 62.17 % of variability, while the first four components accumulated more than 93 % (93.51 %) of the total diversity. Moreover, the dendrogram analysis classified the genotypes within 03 groups.
在阿尔及利亚的市场园地作物中,番茄作物仅次于土豆,占主导地位。本研究的主要目的是评价7(07)个番茄基因型的作物生产性能、某些形态和物候性状的变异及其与产量的相关性。试验采用随机完全区设计(RCBD),于2018年11月至6月在当地无加热温室进行了3个重复。最重要的形态标志是茎生长速度、花序(花数、花序长度)和果实性状(长度和环境)、物候期和平均产量。在所考虑的所有性状中,遗传物质对观察到的变异的影响都是显著的。此外,每两串间产量与茎长、串下叶数、平均单果重、早花期的相关系数均为正值。此外,在作物周期中,开花早与产量表现出非常高且稳定的相关关系(大于0.8)。主成分分析表明,前2个组分产生了62.17%的变异,而前4个组分累积了超过93%(93.51%)的变异。通过树突图分析,对03个组的基因型进行了分类。
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引用次数: 0
EFFECTS OF SILVER NANOPARTICLES ON GROWTH PARAMETERS OF RADISH (Raphanus sativus L. var. radicula). GROWN UNDER DEFICIT IRRIGATION 纳米银对萝卜生长参数的影响。亏缺灌溉
Pub Date : 2022-07-31 DOI: 10.47068/ctns.2022.v11i21.004
Özlem Çakmakci, Talip Çakamakci, S. Şensoy
In agricultural production, fertilization has become mandatory for improving the soil and increasing the yield of the product. Especially in the production of horticultural crops, the need for precise and detailed fertilization management complicates the production. Intensive long-term use of conventional fertilizers causes serious environmental problems such as groundwater pollution, deterioration of soil quality and air pollution. This has led to the search for particularly effective and environmentally friendly fertilizers. Recently, nano-fertilizers are considered as a promising fertilization alternative. Nano-fertilizers benefit nutrition management due to their strong potential to increase nutrient utilization efficiency. The present study aimed to determine the effect of silver nanoparticles (AgNPs) on some growth parameters of radish grown under deficit irrigation conditions. For this purpose, in this study, four different irrigation water levels (I0; 100% full irrigation, I1; 20% deficit irrigation, I2; 40% deficit irrigation and I3; 60% deficit irrigation) and four different nanosilver doses (Ag0: 0 ppm, control; Ag1: 20 ppm; Ag2: 40 ppm and Ag3: 80 ppm) were applied. The study was carried out in a total of 48 pots in three replications according to the randomized plots experimental design under greenhouse conditions. The findings showed that root length, root diameter, root fresh weight and root dry weight significantly (p<0.01) decreased in deficit irrigation. While root parameters were significantly increased in Ag nanoparticle applications, the number of leaves were not varied statistically. The highest root height (33.21 mm) was determined in full irrigation application with Ag3 (80 ppm). As a result, it can be stated that radish plant growth in silver nanoparticles can be significantly improved under deficit irrigation conditions compared to control application non-silver nanoparticles.
在农业生产中,施肥已成为改善土壤和提高产品产量的强制性措施。特别是在园艺作物的生产中,需要精确细致的施肥管理,使生产复杂化。常规肥料的长期集约使用造成地下水污染、土壤质量恶化、大气污染等严重的环境问题。这促使人们寻找特别有效和环保的肥料。近年来,纳米肥料被认为是一种很有前途的施肥选择。纳米肥料具有提高养分利用效率的巨大潜力,有利于营养管理。本研究旨在研究纳米银对亏缺灌溉条件下萝卜部分生长参数的影响。为此,本研究采用四种不同的灌溉水位(0;100%全灌,I1;20%亏缺灌溉,I2;40%亏缺灌溉和I3;60%亏缺灌溉)和四种不同的纳米银剂量(Ag0: 0 ppm,对照;Ag1: 20 ppm;Ag2: 40 ppm和Ag3: 80 ppm)。试验采用温室条件下随机小区试验设计,共设3个重复48个盆栽。结果表明:亏缺灌溉显著降低了根长、根径、根鲜重和根干重(p<0.01);银纳米颗粒处理显著提高了植株的根系参数,但对叶片数量无显著影响。在Ag3 (80 ppm)全灌条件下,根高最高,为33.21 mm。结果表明,在亏缺灌溉条件下,与对照施用非纳米银颗粒相比,纳米银颗粒能显著促进萝卜植株生长。
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引用次数: 0
ASPECTS REGARDING THE IDENTIFICATION OF THE MICROFLORA PRESENT ON FOREST SEEDS INTENDED FOR SOWING IN SPRING 2022 关于鉴定2022年春季播种的森林种子上存在的微生物区系的问题
Pub Date : 2022-07-31 DOI: 10.47068/ctns.2022.v11i21.051
I. Taut, M. Moldovan, V. Șimonca, F. Rebrean, M. Varga, Riti Augustin
Forest crops (nurseries and plantations) impose difficulties on foresters, the main cause being disturbing or harmful factors that compete and interact in different directions, but whose result is the weakening of seedlings, culminating in their death. Given that the number of seedlings in nurseries and plantations is high compared to the unit area, the risk of disease and pest attacks is very high. For this reason, it is necessary to monitor the annual forest crops, both in terms of biological material and soil, in order to control or eliminate possible sources of infestation. In order to achieve the proposed objectives, ash, maple and lime seeds were harvested from Mihai Viteazu Nursery, spruce seeds from Voivodeni Nursery, Forest district Someșu Rece, Forest district Beliș and larch from Forest district Beliș. Specific phytopathological analyzes of the seed samples were performed on the seeds in order to accurately determine the possible pathogens. In the analyzes, the pathogens Fusarium oxysporum, Alternaria alternata and Botrytis cinerea were identified on both deciduous and coniferous seeds.
森林作物(苗圃和种植园)给林农带来了困难,主要原因是令人不安或有害的因素在不同方向上相互竞争和相互作用,但其结果是秧苗变弱,最终导致死亡。由于苗圃和人工林的幼苗数量与单位面积相比很高,因此发生病虫害的风险非常高。为此,有必要从生物材料和土壤两方面对一年生森林作物进行监测,以控制或消除可能的虫害来源。为了实现提议的目标,从Mihai Viteazu苗圃收获了白蜡树、枫树和酸橙种子,从Voivodeni苗圃收获了云杉种子,从Someșu Rece林区收获了云杉种子,从beliuz林区收获了落叶松种子。为了准确地确定可能的病原体,对种子样品进行了特定的植物病理学分析。在分析中,在落叶和针叶种子上都鉴定出了尖孢镰刀菌(Fusarium oxysporum)、互花镰刀菌(Alternaria alternata)和灰霉病菌(Botrytis cinerea)。
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引用次数: 0
CLIMATE ANALYSIS AND WEATHER FORECASTING WITH DATA MINING: THE CASE OF ADANA PROVINCE IN TURKEY 基于数据挖掘的气候分析和天气预报:土耳其阿达纳省的案例
Pub Date : 2022-07-31 DOI: 10.47068/ctns.2022.v11i21.033
Mümine Kaya Keleş, Elif Kavak
In recent years, with the effect of the climate analysis and weather forecasting are accepted as one of the most important natural topics. Adana, located in the Mediterranean region, has a Mediterranean climate and is one of Turkey's roughest cities. Because of its Mediterranean climate, different weather conditions are observed in every season of the year in Adana. In this study, it is aimed to make a monthly average weather forecast for the next 12 months in Adana. The dataset used in this study was collected from two different weather websites since January 2019 and includes Adana's daily weather values including maximum temperature, minimum temperature, humidity, and wind speed. Weather forecasting was performed on this dataset using linear regression method with Weka data mining tool. While predicting the weather using the linear regression method, it is provided that the relationship between the variables was calculated, and an equation between them was established to make predictions. According to the results obtained, it has been observed that good results cannot be obtained when day-based forecasting is requested, but almost the same results are obtained when the monthly average are requested. In addition, the results of the estimations were approximate, and as a result of the study, the Correlation Coefficient was found to be 0.9832.Additionally, it was concluded that while forecasting the weather, linear regression, which is a data mining technique, yields positive results when the forecast is made with the monthly average.
近年来,随着气候的影响,气候分析和天气预报被公认为最重要的自然课题之一。阿达纳位于地中海地区,属于地中海气候,是土耳其最恶劣的城市之一。由于其地中海气候,在阿达纳一年中的每个季节都观察到不同的天气条件。在这项研究中,它的目的是做一个月平均天气预报在阿达纳未来12个月。本研究中使用的数据集是自2019年1月以来从两个不同的天气网站收集的,包括Adana的每日天气值,包括最高温度、最低温度、湿度和风速。利用Weka数据挖掘工具,采用线性回归方法对该数据集进行天气预报。在使用线性回归方法预测天气时,计算了变量之间的关系,建立了变量之间的方程进行预测。根据所获得的结果,已经观察到,当要求以日为基础的预测时,不能得到很好的结果,但当要求以月平均值时,可以得到几乎相同的结果。此外,估计的结果是近似的,研究结果发现相关系数为0.9832。此外,我们还得出结论,在预测天气时,当使用月平均值进行预测时,线性回归(一种数据挖掘技术)会产生积极的结果。
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引用次数: 0
RESEARCH ON OBTAINING LIQUID MYCELIA FROM PLEUROTUS SPP. STRAINS AND TESTING THEIR FRUITING POTENTIAL 侧耳菌液体菌丝的提取及结实性研究
Pub Date : 2022-07-31 DOI: 10.47068/ctns.2022.v11i21.046
Ionut Cristian Rusu, M. Nicolcioiu
The paper focuses on the obtaining biotechnology and in vitro propagation of edible and/or medicinal macromycetes mycelia from the genus Pleurotus in submerged cultures in order to produce the biological “seeding” material - spawn. The quality and biological purity of the spawn is critical to the success of mushroom production and productivity. Starting from stock cultures from the collection of the RDIVFG Vidra, the mother cultures were obtained, the biological material being represented by two strains of Pleurotus ostreatus and one strain of Pleurotus citrinopileatus, each of them being cultivated in PDB + K2HPO4 (1,5g/l), pH 6.5 medium at different stirring speeds (0/100/150 rpm). The submerged growing biomass was used to inoculate wheat caryopsis, that evolved into spawn after complete colonization. The lignocellulosic substrate was obtained from wheat straws with the addition of nutritional supplements (wheat and maize bran and sunflower middlings) and distributed in the polypropylene bags. Their yielding capacity was verified in the fruiting chamber of the laboratory micro-mushroom farm test facility.
本文主要研究了从侧耳菌属(Pleurotus)中获得可食用和(或)药用大型菌丝体的生物技术和体外培养方法,以生产生物“播种”材料——菌种。菌种的质量和生物纯度对蘑菇生产的成功和产量至关重要。从RDIVFG Vidra收集的原始培养物开始,获得母培养物,生物材料为2株平菇(Pleurotus ostreatus)和1株柠檬平菇(Pleurotus citrinopileatus),分别在PDB + K2HPO4 (1,5g/l), pH 6.5的培养基中以不同的搅拌速度(0/100/150 rpm)培养。利用淹没生长生物量接种小麦颖果,颖果在完全定植后进化成卵。木质纤维素底物由麦秆中添加营养添加剂(小麦、玉米麸皮和葵花籽)获得,并分布在聚丙烯袋中。在实验室微蘑菇场试验装置的结果室中验证了它们的生产能力。
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引用次数: 0
EFFECT OF PRE-TREATMENTS ASSISTED MICROWAVE DRYING ON KINETICS AND ENERGY ASPECTS OF DRAGON FRUIT 预处理辅助微波干燥对火龙果动力学和能量方面的影响
Pub Date : 2022-07-31 DOI: 10.47068/ctns.2022.v11i21.008
N. Çetin, Seda Günaydın, Kevser Karaman, C. Sağlam
Drying is one of the most effective preservation methods applied to increase the shelf life of agricultural products. In this study, the effect of different pre-treatments on drying dragon fruit with a microwave was investigated. It is also aimed to determine the drying method with the minimum energy consumption. Dragon fruit slices were dried by microwave at 300 W output power in four different pretreatments as ultrasound, gum arabic, sucrose, and without pretreatment. Pretreatment applications were carried out by performing ultrasound for 10 minutes on dragon fruit slices and soaking them in 10% gum arabic and sucrose solutions before drying. Results showed that the shortest and longest drying times were measured in gum arabic and without pretreatment, respectively. Time-dependent estimation data were calculated by six different thin-layer drying models, considering the drying times and moisture rates. Jena&Das model showed the best fitting performance. As a result, it has been determined that pretreatment applications reduce drying time and energy consumption. In addition, gum arabic pre-treatment is advantageous in terms of energy consumption in microwave drying of dragon fruit.
干燥是延长农产品保质期最有效的保鲜方法之一。研究了不同预处理对火龙果微波干燥的影响。确定能量消耗最小的干燥方法。采用输出功率为300 W的微波干燥火龙果片,分别采用超声波、阿拉伯胶、蔗糖和无预处理四种不同的预处理方法。对火龙果切片进行超声处理10分钟,用10%阿拉伯胶和蔗糖溶液浸泡后干燥。结果表明,阿拉伯胶干燥时间最短,阿拉伯胶干燥时间最长。考虑干燥时间和含水率,采用6种不同的薄层干燥模型计算随时间变化的估计数据。Jena&Das模型的拟合效果最好。结果表明,预处理应用减少了干燥时间和能耗。此外,在火龙果微波干燥过程中,阿拉伯胶预处理在能耗方面具有优势。
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引用次数: 0
DECORATIVE HERBS, A GREEN SOLUTION FOR URBAN ARRANGEMENTS 装饰性草本植物,城市布置的绿色解决方案
Pub Date : 2022-07-31 DOI: 10.47068/ctns.2022.v11i21.039
M. Oprea, D. Giosanu, M. Vulpe
Grasses or ornamental grasses are increasingly used in contemporany arrangements, both in the composition of lawns and as solitary plants, group, masifs or curbs. The present study was conducted to evaluate the behavior of some decorative grass species/cultivars: Carex morrowii ΄Snowline΄, Carex morowii ΄Ice Dance΄, Festuca glauca, Koeleria glauca, Miscanthus sinensism ΄Variegatus΄, Pennisetum alopecuroides ΄Hameln΄, Pennisetum setaceum ΄Rubrum΄, in different areas of Pitesti. Observations and determinations were made on: biological characteristics, ability to multiply, aesthetic qualities, growing and requirements, winter hardiness, pruning effects, correlated with the variation of everage annual temperatures (°C) and average annual rainfall (mm/month) in 2 year of reporting, 2019 and 2020. Ways have been proposed to use the ornamental grasses studied in the landscape arrangements and the importance of their incorporation in the green spaces, for ecosystem offers: restoring the ecological balance, capitalizing on natural resources and developing biodiversity.
草或观赏草越来越多地用于现代安排,无论是在草坪的组成和作为单独的植物,集团,块状或镶边。本研究对湖北不同地区的几种装饰草品种(Carex morrowii ΄Snowline΄、Carex morowii ΄Ice Dance΄、Festuca glauca、Koeleria glauca、Miscanthus sinensis ΄Variegatus΄、Pennisetum alopecuroides ΄Hameln΄、Pennisetum setaceum ΄Rubrum΄)的行为进行了评价。在2019年和2020年的2个报告年度中,对生物特性、繁殖能力、美学品质、生长和需求、耐寒性、修剪效果以及与年平均气温(°C)和年平均降雨量(mm/月)变化的相关性进行了观察和确定。研究人员提出了在景观布置中使用观赏草的方法,以及将观赏草纳入绿色空间的重要性,以提供生态系统:恢复生态平衡,利用自然资源和发展生物多样性。
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引用次数: 0
A SURVEY OF PRESERVED SPECIMENS OF THE GENUS FESTUCA L. (POACEAE) IN ROMANIA 标题罗马尼亚羊蹄草属保存标本的调查
Pub Date : 2022-07-31 DOI: 10.47068/ctns.2022.v11i21.058
D. Vasile, L. Dincă, R. Enescu
The genus Festuca L. is a genus of flowering plants belonging to the grass family Poaceae, being one of the largest in Gramineae with ±450 species in temperate and alpine regions of both hemispheres with a cosmopolitan distribution occurring on every continent except Antarctica. They are evergreen or herbaceous perennial tufted grasses with a height range of 10–200 cm. The specimens of Festuca are preserved in the Al Beldie Herbarium from INCDS Marin Drăcea Bucharest. This Herbarium hosted 309 vouchers with 52 Festuca species, most of them belonging to F. gigantea (28), F. versicolor (27) and F. arundinacea (22). The oldest specimens were collected in 1773 and 1788. Most of the herbarium specimens were generally in very good (198) and good (76) condition except for a smaller number of specimens which were damaged (35). The aim of this article is to describe some Festuca species which are very good preserved.
羊茅属(Festuca L.)是禾本科开花植物属,是禾本科最大的植物属之一,在南北半球温带和高寒地区共有约450种,分布在除南极洲以外的各大洲。它们是常绿或草本多年生丛状草,高度范围为10-200厘米。Festuca的标本保存在布加勒斯特Marin drcea INCDS的Al Beldie植物标本室。本标本室共收录羊茅属植物52种309种,主要属F. gigantea(28种)、F. versicolor(27种)和F. arundinacea(22种)。最古老的标本是在1773年和1788年收集的。除少部分标本受损外,绝大多数标本的保存状况为良好(198件)和良好(76件)。本文的目的是描述一些保存完好的羊茅属植物。
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引用次数: 1
RESEARCH ON THE QUALITY OF TOMATOES GROWN WITH VERMICOMPOST FERTILIZATION, SOLARIUM CULTIVATION 蚯蚓堆肥日光栽培番茄品质的研究
Pub Date : 2022-07-31 DOI: 10.47068/ctns.2022.v11i21.030
V. Ilie, M. Mihalache
The nutrition regime for tomatoes is very complex and must consider the specific consumption that differs depending on the variety, vegetation phase, cropping system and others. The super-intensive character of the protected and forced vegetable crops determines a special specificity regarding the requirements towards the soil and its state of supply with nutrients. Vermicompost, also known under the name of earthworm humus, is an organic fertilizer that is produced utilizing the assistance of earthworms. Vermicompost can completely replace any other chemical or even organic fertilizer, as it contains, up to 100 times more nutrients and microorganisms that are beneficial for plants. The researches have been carried out since 2020 in Matca, Galati County and aimed at producing vermicompost and testing it on different crops in protected areas. Tomatoes - Yigido F1 is a hybrid of semi-early tomatoes, with undetermined growth, intended for cultivation in protected areas. There were made determinations on tomatoes culture regarding the influence of vermicompost on the development of plants and quality of tomatoes.
番茄的营养体系非常复杂,必须考虑到根据品种、植被阶段、种植制度和其他因素而不同的具体消费量。受保护和强制种植蔬菜作物的超集约化特征决定了其对土壤及其养分供应状况的特殊要求。蚯蚓堆肥,也被称为蚯蚓腐殖质,是一种利用蚯蚓的帮助生产的有机肥料。蚯蚓堆肥可以完全取代任何其他化学肥料甚至有机肥料,因为它含有高达100倍的对植物有益的营养物质和微生物。该研究自2020年以来在加拉蒂县的Matca进行,旨在生产蚯蚓堆肥,并在保护区的不同作物上进行测试。番茄——Yigido F1是一种半早熟番茄的杂交品种,生长情况不确定,拟在保护区种植。对番茄栽培中蚯蚓堆肥对番茄植株发育和品质的影响进行了测定。
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引用次数: 0
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Current Trends in Natural Sciences
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