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Antibacterial Activity of Ethanolic Extract Siwak Stem (Salvadora persica) Against Staphylococcus aureus ATCC 25923 西瓦克茎乙醇提取物对金黄色葡萄球菌ATCC 25923的抑菌活性研究
Pub Date : 2023-02-01 DOI: 10.33830/manilkara.v1i2.4095.2023
Nisa’ Nur Sholikhah, Andang Arif Wibawa, R. M. Rukmana
Infection is caused by bacteria, one of which is Staphylococcus aureus. Infectious treatment can use Siwak as natural plants (Salvadora persica), namely Siwak plants. Salvadora persica contains Saponins, Alkaloids, Tanins, and Fenols. This study is to know the antibacterial activity of Salvadora persica stems against Staphylococcus aureus ATCC 25923 growth. Extraction method using maceration with ethanol as a solvent. The group of extract compounds was identified using various chemical reagents. Salvadora persica stem extract was made in various concentrations of 6,25%, 12,5%, 25%, and 50% by using dimethyl sulfoxide 2% (DMSO) as thinner. Chlorhexidine 0,2% was a positive control, and DMSO 2% was a negative control. Antibacterial activity test was obtained by diffusion method. Analysis data used way ANOVA test. The results ethanolic extract of the siwak stem contains a class of compounds: saponins, alkaloids, tannins, and phenols. The result showed that Salvadora persica stem extracts antibacterial activity against Staphylococcus aureus ATCC 25923. Antibacterial activity test against Staphylococcus aureus from laboratory culture showed an average of largest inhibition zone diameter on 50% concentration with 20,5 mm diameter and smallest inhibition zone at 6,25% concentration with 14 mm diameter.
感染是由细菌引起的,其中一种是金黄色葡萄球菌。感染治疗可以利用西瓦克作为天然植物(萨尔瓦多),即西瓦克植物。番石榴含有皂苷、生物碱、单宁和茴香醇。本研究旨在了解番石榴茎对金黄色葡萄球菌ATCC 25923生长的抑菌活性。以乙醇为溶剂浸渍的提取方法。用不同的化学试剂对提取物进行了鉴定。以2%二甲亚砜(DMSO)为稀释剂,分别以6、25%、12、5%、25%、50%的浓度制备木耳茎提取物。氯己定0.2%为阳性对照,DMSO 2%为阴性对照。采用扩散法测定其抑菌活性。数据分析采用方差分析方法。结果木犀草醇提物中含有皂苷、生物碱、单宁和酚类化合物。结果表明,木香茎提取物对金黄色葡萄球菌ATCC 25923具有抑菌活性。对实验室培养金黄色葡萄球菌的抑菌活性试验表明,50%浓度时平均抑菌带直径最大,为20.5 mm; 6.25%浓度时平均抑菌带直径最小,为14 mm。
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引用次数: 0
Modification of Root Staining Preparation Method for Detection And Visualization Colony Formation of Arbuscular Mycorhriza Fungi (AMF) 根染色制备方法对丛枝菌根真菌菌落形成检测及可视化的改进
Pub Date : 2022-08-31 DOI: 10.33830/manilkara.v1i1.3166.2022
Susan Irmayanti, Inggit Winarni
The root staining method was used to detect the presence of Arbuscular Mycorrhizal Fungi (AMF) and to calculate the colonization of the AMF in the roots. Optimal root staining methods not only help in the study of plant-associated mycorrhizae, but also save time and money. Root staining aims to clarify and sharpen the picture of AMF associated with plants on the roots, making it easier to observe with a light microscope. This study aims to obtain an effective (fast, safe, and economical) and flexible method of preparation of root staining so that the visualization of AMF on roots becomes clear and contrasting. The method used in this research is descriptive qualitative which includes the cleanliness of the roots from cell contents, root texture, and color contrast, which consists of three treatments, namely P1 (control) using 10% KOH with 90°C heating and followed by the modified Philips & Hayman staining procedure (1970), P2 using 5% KOH by heating at 90°C followed by the modified Philips & Hayman (1970) procedure using 1% HCl, and P3 using 5% KOH heating at 90°C then stained followed the staining method of Philips & Hayman (1970) which was modified by the use of commercial vinegar as a substitute for HCl. All treatments used Trypan Blue dye. The results showed that the P2 and P3 treatments showed results that were not different from P1, the state of the roots is clean enough thereby detection and visualization of AMF could still be observed properly. The P3 treatment method with heating for 9 minutes can be an alternative method that is effective (fast, safe, economical) and flexible. It faster than common method because it takes 9 minutes for cleaning root cells, relatively safe with the use of commercial vinegar solution to replace HCl solution, and economical because it can reduce the need for KOH material up to 50%.
采用根染色法检测丛枝菌根真菌(AMF)的存在,计算AMF在根中的定殖量。最佳的根染色方法不仅有助于植物相关菌根的研究,而且节省了时间和金钱。根染色旨在澄清和锐化根上与植物相关的AMF图像,使其更容易用光学显微镜观察。本研究旨在获得一种有效(快速、安全、经济)、灵活的根染色制备方法,使AMF在根上的可视化清晰、对比鲜明。本研究中使用的方法是描述性定性的,包括根从细胞内容物、根质地和颜色对比的清洁度,包括三种处理,即P1(对照)使用10%的KOH, 90°C加热,然后使用改良的Philips & Hayman染色程序(1970),P2使用5%的KOH, 90°C加热,然后使用改良的Philips & Hayman(1970)程序,P3用5% KOH在90°C下加热,然后按照Philips & Hayman(1970)的染色方法进行染色,该方法通过使用商用醋代替HCl进行了改进。所有处理均使用台盼蓝染料。结果表明,P2和P3处理的结果与P1处理没有差异,根系状态足够干净,仍然可以很好地观察到AMF的检测和可视化。加热9分钟的P3治疗方法是一种有效(快速、安全、经济)、灵活的替代方法。它比普通方法快,因为清洗根细胞只需要9分钟,与使用商业醋溶液代替HCl溶液相比相对安全,而且经济,因为它可以减少对KOH材料的需求高达50%。
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引用次数: 0
Diversity of Macrozoobentos as Bioindicator of Lentic Waters in The Jombor Swamp Area Klaten Central Java 中爪哇岛Jombor沼泽地区大型动物类生物指示物的多样性
Pub Date : 2022-08-31 DOI: 10.33830/manilkara.v1i1.3394.2022
Sandhie Budi Himawan, Yuni Tri Hewindati
Organisms that live permanently on the bottom of lentic or still waters, such as macrozoobenthos, are very susceptible to changes in water quality that can have an impact on their composition and abundance. Because of their tolerance to environmental changes, these organisms are often used as an indicator in physical and chemical changes in the aquatic environment. Rawa Jombor or Jombor swamp is a lentic freshwater in Krakitan Village, Bayat District, Klaten, Central Java. In addition to tourism, the surrounding community uses Rawa Jombor in their daily activities for floating market and fish cages. The existence of these various activities can produce waste and garbage which then settles to the bottom of the waters and has an impact on reducing water quality. This study aims to examine the diversity and distribution of macrozoobenthos. Water quality was measured by taking samples at three locations, namely station I (littoral zone), station II (limnetic zone), and station III (benthic zone). Sampling at each station used a Van Veen grab sediment sampler. The Macrozoobenthos Diversity Index was determined using the Shannon & Wiener formula. The diversity index values for stations I, II, and III are 1.579; 1.566; and 1.785, respectively, with moderate levels of community diversity. 14 types of macrozoobenthos were identified, of which Tubifex tubifex was the most abundant species in station 1 and station 2. Meanwhile at station 3 is dominated by Anentome Helena. Based on the analysis of organism diversity and the results of water quality inspection in our study, it shows that the water quality in the Jombor swamp is classified as moderately polluted.
永久生活在静水或静水底部的生物,如大型底栖动物,非常容易受到水质变化的影响,这可能对它们的组成和丰度产生影响。由于它们对环境变化的耐受性,这些生物经常被用作水生环境物理和化学变化的指示物。Rawa Jombor或Jombor沼泽是中爪哇Klaten Bayat区Krakitan村的一个真正的淡水。除了旅游之外,周边社区在日常活动中还利用拉瓦琼堡作为水上市场和鱼笼。这些不同活动的存在会产生废物和垃圾,这些废物和垃圾会沉降到水底,对降低水质产生影响。本研究旨在探讨大型底栖动物的多样性和分布。在1号站(滨海带)、2号站(滨海带)和3号站(底栖带)3个地点取样,测量水质。每个站点使用Van Veen抓取沉淀物采样器进行采样。大型底栖动物多样性指数采用Shannon & Wiener公式确定。1、2、3站的多样性指数为1.579;1.566;群落多样性为1.785,群落多样性水平中等。共鉴定出大型底栖动物14种,其中1站和2站数量最多的是Tubifex Tubifex。与此同时,3号站由海伦娜主宰。根据本研究的生物多样性分析和水质检测结果表明,Jombor沼泽的水质属于中度污染。
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引用次数: 0
Aktivitas Antibakteri Ekstrak Etanolik Daun Jeruk (Citrus nobilis, Citrus sinensis, dan Citrus maxima) Terhadap Bakteri Staphylococcus aureus dan Pseudomonas aeruginosa 柑橘叶蓝素提取物(Citrus nobilis, Citrus sinensis和Citrus maxima)抗菌活性可用于葡萄球菌aurecus和尿绿假单胞菌
Pub Date : 2022-08-31 DOI: 10.33830/manilkara.v1i1.3168.2022
Ifandari, Einstivina Nuryandani
Bakteri Staphylococcus aureus dan Pseudomonas aeruginosa dapat menjadi patogen pada manusia. Bakteri ini diketahui bersifat resisten terhadap antibiotik tertentu. Salah satu bahan alam yang berpotensi memiliki aktivitas antibakteri sebagai alternatif penggunaan antibiotik adalah daun jeruk. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak daun jeruk dari tiga jenis jeruk (Citrus nobilis, Citrus sinensis, and Citrus maxima) terhadap pertumbuhan bakteri S. aureus dan P. aeruginosa. Penelitian ini menggunakan metode difusi cakram kertas (paper disk) dengan persentase 12,5%, 25%, dan 50% ekstrak yang dilarutkan dalam karboksimetil selulosa (CMC) 2%. Kontrol negatif menggunakan CMC 2%, sedangkan kontrol positif menggunakan antibiotik siprofloksasin. Hasil penelitian menunjukkan bahwa aktifitas antibakteri ketiga macam ekstrak berbeda signifikan dengan kontrol negatif, namun aktifitas antara ketiga macam ekstrak daun jeruk tidak berbeda secara signifikan. Ekstrak daun C. nobilis dengan konsentrasi 50% memiliki aktivitas antibakteri S. aureus relatif lebih tinggi dibandingkan kedua ekstrak daun jeruk yang lain, sedangkan pada bakteri P. aeruginosa aktivitas antibakteri ekstrak daun C. maxima relatif lebih tinggi daripada ekstrak daun jeruk yang lain. Hasil ini menunjukkan bahwa ekstrak daun jeruk dari C. nobilis, C. sinensis, dan C. maxima memiliki daya hambat terhadap pertumbuhan S. aureus dan P. aeruginosa.Bakteri Staphylococcus aureus dan Pseudomonas aeruginosa dapat menjadi patogen pada manusia. Bakteri ini diketahui bersifat resisten terhadap antibiotik tertentu. Salah satu bahan alam yang berpotensi memiliki aktivitas antibakteri sebagai alternatif penggunaan antibiotik adalah daun jeruk. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak daun jeruk dari tiga jenis jeruk (Citrus nobilis, Citrus sinensis, and Citrus maxima) terhadap pertumbuhan bakteri S. aureus dan P. aeruginosa. Penelitian ini menggunakan metode difusi cakram kertas (paper disk) dengan persentase 12,5%, 25%, dan 50% ekstrak yang dilarutkan dalam karboksimetil selulosa (CMC) 2%. Kontrol negatif menggunakan CMC 2%, sedangkan kontrol positif menggunakan antibiotik siprofloksasin. Hasil penelitian menunjukkan bahwa aktifitas antibakteri ketiga macam ekstrak berbeda signifikan dengan kontrol negatif, namun aktifitas antara ketiga macam ekstrak daun jeruk tidak berbeda secara signifikan. Ekstrak daun C. nobilis dengan konsentrasi 50% memiliki aktivitas antibakteri S. aureus relatif lebih tinggi dibandingkan kedua ekstrak daun jeruk yang lain, sedangkan pada bakteri P. aeruginosa aktivitas antibakteri ekstrak daun C. maxima relatif lebih tinggi daripada ekstrak daun jeruk yang lain. Hasil ini menunjukkan bahwa ekstrak daun jeruk dari C. nobilis, C. sinensis, dan C. maxima memiliki daya hambat terhadap pertumbuhan S. aureus dan P. aeruginosa.
肺气肿葡萄球菌和假单胞菌可以成为人类的病原体。众所周知,这种细菌对某些抗生素有抗药性。另一种可能的天然抗菌活性是柑橘叶,而不是抗生素。这项研究的目的是确定三种橙子提取物(Citrus nobilis, Citrus sinensis和Citrus maxima)对S. aureus和P. aeruginosa细菌的抑制能力。该研究采用的是纸质圆盘的扩散方法,其含量为12.5%、25%和50%,苯酚甲基纤维素(CMC)中溶解的提取物为2%。负控制使用CMC 2%,正控制使用西佛洛辛抗生素。研究表明,三种不同的抗菌活性与消极控制不同,但三种不同的柑橘叶提取物之间的活动明显不同。含50%浓度的叶甘提取物具有抗菌活性,而丙烯酸p.uginosa抗菌活性比其他橙子提取物相对较高。这一结果表明,来自C. nobilis, C. sinensis和C. maxima的柑橘叶提取物具有抑制S. aureus和P. aerginosa发育的能力。肺气肿葡萄球菌和假单胞菌可以成为人类的病原体。众所周知,这种细菌对某些抗生素有抗药性。另一种可能的天然抗菌活性是柑橘叶,而不是抗生素。这项研究的目的是确定三种橙子提取物(Citrus nobilis, Citrus sinensis和Citrus maxima)对S. aureus和P. aeruginosa细菌的抑制能力。该研究采用的是纸质圆盘的扩散方法,其含量为12.5%、25%和50%,苯酚甲基纤维素(CMC)中溶解的提取物为2%。负控制使用CMC 2%,正控制使用西佛洛辛抗生素。研究表明,三种不同的抗菌活性与消极控制不同,但三种不同的柑橘叶提取物之间的活动明显不同。含50%浓度的叶甘提取物具有抗菌活性,而丙烯酸p.uginosa抗菌活性比其他橙子提取物相对较高。这一结果表明,来自C. nobilis, C. sinensis和C. maxima的柑橘叶提取物具有抑制S. aureus和P. aerginosa发育的能力。
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引用次数: 1
Coral Reef Ecosystem Potential in Gili Sulat and Gili Lawang Water Tourism Park East Lombok 东龙目岛吉利苏拉特和吉利拉旺水上旅游公园的珊瑚礁生态系统潜力
Pub Date : 2022-08-31 DOI: 10.33830/manilkara.v1i1.3401.2022
Lalu Ainul Akhyar, B. Prasetyo
Coral reefs are one of the ecosystems that play an important role in coastal areas but are vulnerable to change. Ecologically, coral reefs act as habitats for various kinds of reef fish, in addition to functioning as beach protectors from the crashing waves of currents as well as a source of germplasm. The diversity of coral genera and other biota is one of the important indicators in supporting the sustainability of water tourism activities, especially snorkeling and diving. Areas that have relatively high diversity of coral reefs have more resilience in their role in protecting coastal areas so that their existence needs to be maintained in order to avoid extinction. This study aims to identify the names of coral genera and measure the percentage of coral cover in the Gili Sulat and Gili Lawang Water Tourism Parks (WTP). The method used in this research is the Point Intercept Transect (PIT) method and the Time Swim method. The results showed that 10 genera had been identified, namely the genus Acropora, Euphyllia, Fungia, Galaxea, Lobophyllia, Montastrea, Montipora, Pachyseris, Plerogyra, and Porites. The largest coral genera were Porites and Acropora which were spread on transects 1 and 7. The average coverage of the two corals was 32.34% in Gili Sulat and 39.64% in Gili Lawang.
珊瑚礁是沿海地区重要的生态系统之一,但极易受到变化的影响。从生态学上讲,珊瑚礁是各种珊瑚鱼的栖息地,除了作为海滩的保护层,免受海浪的冲击,也是种质资源的来源。珊瑚属和其他生物群的多样性是支持水上旅游活动(特别是浮潜和潜水)可持续性的重要指标之一。珊瑚礁多样性相对较高的地区在保护沿海地区方面具有更强的复原力,因此需要维持它们的存在,以避免灭绝。本研究旨在确定Gili Sulat和Gili Lawang水上旅游公园(WTP)的珊瑚属名称和测量珊瑚覆盖百分比。本研究使用的方法是点截样条法(PIT)和时间游法。结果表明,共鉴定出10个属,分别为Acropora属、Euphyllia属、Fungia属、Galaxea属、Lobophyllia属、monastrea属、Montipora属、Pachyseris属、Plerogyra属和Porites属。分布在样条1和样条7上的珊瑚属以Porites和Acropora最多。Gili Sulat和Gili Lawang两种珊瑚的平均覆盖率分别为32.34%和39.64%。
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引用次数: 1
Maize Morphological Variability in Ciseeng District, Bogor Regency and Setu District, South Tangerang City 南坦格朗市茂物县Ciseeng区和setuu区的玉米形态变异
Pub Date : 2022-08-31 DOI: 10.33830/manilkara.v1i1.3546.2022
Diki, Susi Sulistiana, Arif Cahyani Ilyas
Penelitian ini mengukur dan membandingkan variabilitas morfologi dari dua populasi jagung varietas Golden di Kabupaten Bogor dan Kota Tangerang Selatan dengan mengukur parameter morfologi tanaman jagung yaitu: jumlah tongkol panen, tinggi tanaman, tinggi letak tongkol, panjang tongkol, diameter tongkol, kadar air saat panen dan bobot kering 100 biji. Pupuk yang digunakan berbeda, untuk tanaman jagung di lokasi kota Tangerang Selatan menggunakan pupuk organik yaitu pupuk kandang kambing, sedangkan lokasi kabupaten Bogor menggunakan pupuk anorganik yaitu pupuk urea, TSP, KCl (NPK). Untuk menganalisis data variabilitas menggunakan Anova. Berdasarkan hasil pengamatan, bahwa secara umum terdapat perbedaan dalam morfologi jagung diantara kedua lokasi tersebut. Morfologi jagung di lokasi kota Tangerang Selatan dengan kabupaten Bogor berbeda terutama dalam hal, variabel tinggi tongkol, diameter tongkol, bobot 100 biji, bobot tongkol, dan kadar air. Perbedaan hasil tersebut diduga karena penggunaan pupuk yang berbeda.
这项研究通过测量玉米的两种金质玉米品种的形态变化来测量和比较,通过测量玉米的形态参数,即收获玉米穗的数量、植物的高度、穗子的位置、穗子的长度、穗子的直径、收获时的含水率和100个种子的干重量。在Tangerang市南部的玉米中,使用不同的肥料,如有机肥料,如羊圈,而茂物区则使用无机肥料如尿素,TSP, KCl (NPK)。使用Anova分析数据变异性。观察结果显示,玉米的形态在这两个地点之间普遍存在差异。在Tangerang城和Bogor区南部的玉米形态学主要在不同的方面,高变量穗子、圆穗子直径、100颗种子的重量、穗子的重量和水温。这些结果的不同被认为是使用了不同的肥料。
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引用次数: 0
期刊
MANILKARA: Journal of Bioscience
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