Pub Date : 2025-11-19DOI: 10.1016/j.biomaterials.2025.123863
Zheng Xie , Zehao Chen , Weifeng Wu , Miaomiao Fei , Wenting Li , Silu Cao , Nan Wang , Xiaoyue Xu , Xiaoxiao Sun , Qiang Liu , Xiao-fei Gao , Cheng Li
Perioperative neurocognitive disorder (PND) is a common complication in older surgical patients, leading to increased neurodegenerative and death risk, augment socioeconomic burdens. Despite its prevalence, the reasons of why this complication occurs highly in older, underlying pathogenesis mechanisms, and effective treatments remain unclear. Senescence associated secretory phenotype (SASP), resulting from cellular senescence, drives inflammaging and cognitive decline. However, the association between cellular senescent and poor cognitive outcome is seldomly defined in PND. Herein, we showed that anesthesia and surgery in aged mice further increase hippocampal neuron senescent burden, manifest as increased senescence-like markers (CDKN2A/p16, CDKN1A/p21, SASP, SA-β-Gal), along with lipofuscin and lipid droplet accumulation and synaptic dysfunction. We identified elevated PF4, a platelet-derived factor, as a defensive response in older PND mice. Intraperitoneal PF4 administration mitigated neuronal senescence burden and improved cognitive dysfunction. Considering the older, frail patients and shorter perioperative period, we developed microfluidic hydrogel microspheres and cationic thermosensitive hydrogel complexes for nasal PF4 delivery enabling satisfy minimally invasive, less frequent dosing and sustained treatment. These findings reveal a critical role for cellular senescence in PND and propose PF4-based therapies as a promising translational strategy.
{"title":"Target anesthesia and surgery induced senescent cell via composite microsphere hydrogels as nose-to-brain amplifiers of platelet factor 4 delivery for neurocognitive dysfunction repair","authors":"Zheng Xie , Zehao Chen , Weifeng Wu , Miaomiao Fei , Wenting Li , Silu Cao , Nan Wang , Xiaoyue Xu , Xiaoxiao Sun , Qiang Liu , Xiao-fei Gao , Cheng Li","doi":"10.1016/j.biomaterials.2025.123863","DOIUrl":"10.1016/j.biomaterials.2025.123863","url":null,"abstract":"<div><div>Perioperative neurocognitive disorder (PND) is a common complication in older surgical patients, leading to increased neurodegenerative and death risk, augment socioeconomic burdens. Despite its prevalence, the reasons of why this complication occurs highly in older, underlying pathogenesis mechanisms, and effective treatments remain unclear. Senescence associated secretory phenotype (SASP), resulting from cellular senescence, drives inflammaging and cognitive decline. However, the association between cellular senescent and poor cognitive outcome is seldomly defined in PND. Herein, we showed that anesthesia and surgery in aged mice further increase hippocampal neuron senescent burden, manifest as increased senescence-like markers (CDKN2A/p16, CDKN1A/p21, SASP, SA-β-Gal), along with lipofuscin and lipid droplet accumulation and synaptic dysfunction. We identified elevated PF4, a platelet-derived factor, as a defensive response in older PND mice. Intraperitoneal PF4 administration mitigated neuronal senescence burden and improved cognitive dysfunction. Considering the older, frail patients and shorter perioperative period, we developed microfluidic hydrogel microspheres and cationic thermosensitive hydrogel complexes for nasal PF4 delivery enabling satisfy minimally invasive, less frequent dosing and sustained treatment. These findings reveal a critical role for cellular senescence in PND and propose PF4-based therapies as a promising translational strategy.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"328 ","pages":"Article 123863"},"PeriodicalIF":12.9,"publicationDate":"2025-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145601603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-17DOI: 10.1016/j.biomaterials.2025.123859
Hui Zhi , Weimin Yin , Shiyu Chen , Xiaoyou Zhang , Zichen Yang , Fulong Man , Rongjie Li , Yanni Cai , Yang Li , Caoyi You , Yan Li , Yongyong Li , Haiqing Dong
{"title":"Corrigendum to “Lactate metabolism regulating nanosystem synergizes cuproptosis and ferroptosis to enhance cancer immunotherapy” [Biomaterials 325 (2026) 123538]","authors":"Hui Zhi , Weimin Yin , Shiyu Chen , Xiaoyou Zhang , Zichen Yang , Fulong Man , Rongjie Li , Yanni Cai , Yang Li , Caoyi You , Yan Li , Yongyong Li , Haiqing Dong","doi":"10.1016/j.biomaterials.2025.123859","DOIUrl":"10.1016/j.biomaterials.2025.123859","url":null,"abstract":"","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"327 ","pages":"Article 123859"},"PeriodicalIF":12.9,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145547491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-15DOI: 10.1016/j.biomaterials.2025.123856
Peiran Chen , Chao Qi , Ke Zhang , Shupeng Wang , Xiaotong Lu , Kun Qian , Mingyue Zhang , Shiyao Guo , Yuhong Zhuo , Chenguo Yao , Kaiyong Cai
Piezocatalytic therapy, which utilizes ultrasonic activation of piezoelectric materials to generate reactive oxygen species (ROS), holds significant potential. However, its efficacy is constrained by the limited ROS generation capacity of piezoelectric materials. In this study, a gradient ion replacement strategy was employed to construct CuxBa1-xTiO3-shell structured BaTiO3 (Cu-BTO) piezoelectric materials with flexoelectric properties. This process induces the BTO surface to transition from a crystalline state to an amorphous state and subsequently recrystallize. The phase transformation introduces flexoelectric properties in Cu-BTO surface, while the disparity in ionic radii between Cu2+ and Ba2+ enhances lattice asymmetry. Consequently, Cu-BTO exhibits significantly enhanced piezoelectric and piezocatalytic properties, with the d33 value reaching 129.91 pm/V, representing an increase of 345.93 %. Under ultrasonic stimulation, Cu-BTO can not only directly generate •OH and H2O2 through piezocatalysis, but also achieve self-amplified Fenton-like catalysis and GSH depletion by promoting charge transfer via the built-in electric field. The strong oxidative stress induces severe immunogenic cell death (ICD) of tumor cells, and triggers a series of antitumor immune responses such as dendritic cell (DC) maturation and T cell activation. Ultimately, an 83.7 % tumor inhibition rate is achieved, and lung metastasis of the tumor is effectively prevented. This work not only demonstrates a method to describes a method for inducing the flexoelectric effect in piezoelectric nanomaterials but also provides novel insights into the design and optimization of piezoelectric nanomaterials.
{"title":"Flexoelectricity-boosted core-shell nanoparticles with self-amplified Fenton-like activity for tumor piezocatalytic immunotherapy","authors":"Peiran Chen , Chao Qi , Ke Zhang , Shupeng Wang , Xiaotong Lu , Kun Qian , Mingyue Zhang , Shiyao Guo , Yuhong Zhuo , Chenguo Yao , Kaiyong Cai","doi":"10.1016/j.biomaterials.2025.123856","DOIUrl":"10.1016/j.biomaterials.2025.123856","url":null,"abstract":"<div><div>Piezocatalytic therapy, which utilizes ultrasonic activation of piezoelectric materials to generate reactive oxygen species (ROS), holds significant potential. However, its efficacy is constrained by the limited ROS generation capacity of piezoelectric materials. In this study, a gradient ion replacement strategy was employed to construct Cu<sub>x</sub>Ba<sub>1-x</sub>TiO<sub>3</sub>-shell structured BaTiO<sub>3</sub> (Cu-BTO) piezoelectric materials with flexoelectric properties. This process induces the BTO surface to transition from a crystalline state to an amorphous state and subsequently recrystallize. The phase transformation introduces flexoelectric properties in Cu-BTO surface, while the disparity in ionic radii between Cu<sup>2+</sup> and Ba<sup>2+</sup> enhances lattice asymmetry. Consequently, Cu-BTO exhibits significantly enhanced piezoelectric and piezocatalytic properties, with the <em>d</em><sub><em>33</em></sub> value reaching 129.91 pm/V, representing an increase of 345.93 %. Under ultrasonic stimulation, Cu-BTO can not only directly generate <sup>•</sup>OH and H<sub>2</sub>O<sub>2</sub> through piezocatalysis, but also achieve self-amplified Fenton-like catalysis and GSH depletion by promoting charge transfer via the built-in electric field. The strong oxidative stress induces severe immunogenic cell death (ICD) of tumor cells, and triggers a series of antitumor immune responses such as dendritic cell (DC) maturation and T cell activation. Ultimately, an 83.7 % tumor inhibition rate is achieved, and lung metastasis of the tumor is effectively prevented. This work not only demonstrates a method to describes a method for inducing the flexoelectric effect in piezoelectric nanomaterials but also provides novel insights into the design and optimization of piezoelectric nanomaterials.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"328 ","pages":"Article 123856"},"PeriodicalIF":12.9,"publicationDate":"2025-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145555908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-15DOI: 10.1016/j.biomaterials.2025.123860
Chenlu Yao , Qingle Ma , Heng Wang , Bingbing Wu , Huaxing Dai , Jialu Xu , Jinyu Bai , Fang Xu , Admire Dube , Chao Wang
{"title":"Corrigendum to “Targeting myeloid cells with platelet-derived extracellular vesicles to overcome resistance of immune checkpoint blockade therapy” [Biomaterials 321 (2025) 123336]","authors":"Chenlu Yao , Qingle Ma , Heng Wang , Bingbing Wu , Huaxing Dai , Jialu Xu , Jinyu Bai , Fang Xu , Admire Dube , Chao Wang","doi":"10.1016/j.biomaterials.2025.123860","DOIUrl":"10.1016/j.biomaterials.2025.123860","url":null,"abstract":"","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"327 ","pages":"Article 123860"},"PeriodicalIF":12.9,"publicationDate":"2025-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145533822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-14DOI: 10.1016/j.biomaterials.2025.123852
Anthony J. Bullock , David A. Gregory , Raouf Seyam , Falah Al-Mohanna , Omar Alsulaiman , Victoria L. Workman , Cornelia Rodenburg , Waleed Altaweel , Christopher R. Chapple , Sheila MacNeil
Although polypropylene (PP) materials have been implanted for decades for urethral support in the pelvic floor, appropriate large animal models and advanced materials analysis techniques have not previously been used to investigate the clinical problems they can cause - inflammation, pain and erosion through tissues.
An ovine model duplicating the surgical procedure for suburethral sling surgery was developed. Here we present the results after 3 months implantation using immunohistochemistry and advanced materials characterisation of two materials PP and Polyurethane (PU).
Both materials were well integrated into the tissue. The M1/M2 ratio in PP-implanted tissue was statistically significantly elevated (4.29) compared to PU (0.63) and control tissue (0.34). The higher ratio indicates a more inflammatory response to PP than PU.
Surface roughness (assessed using atomic force microscopy) increased in both materials, Rq from 5.73 to 10.2 nm in PP and from 1.03 to 2.96 nm in PU; whilst Ra went from 4.75 to 7.85 nm in PP and from 0.81 to 2.36 nm in PU. Notably, surface stiffness increased by 0.05 GPa in PP and decreased by 0.2 GPa in PU. PP underwent both surface and bulk material degradation, PU did not.
Detailed testing of implantable materials in an appropriate animal model should be conducted before materials are introduced into clinical practice. It is salutary that this has never been reported before. The use of material characterisation techniques allowed us to identify problems in the performance of PP, notably surface degradation, changes in bulk properties and stiffening, which can activate macrophages. In contrast, PU appears a more suitable alternative material for use in treating patients with SUI.
{"title":"A critical comparison of polypropylene and polyurethane sling materials after implantation in a suburethral sheep model","authors":"Anthony J. Bullock , David A. Gregory , Raouf Seyam , Falah Al-Mohanna , Omar Alsulaiman , Victoria L. Workman , Cornelia Rodenburg , Waleed Altaweel , Christopher R. Chapple , Sheila MacNeil","doi":"10.1016/j.biomaterials.2025.123852","DOIUrl":"10.1016/j.biomaterials.2025.123852","url":null,"abstract":"<div><div>Although polypropylene (PP) materials have been implanted for decades for urethral support in the pelvic floor, appropriate large animal models and advanced materials analysis techniques have not previously been used to investigate the clinical problems they can cause - inflammation, pain and erosion through tissues.</div><div>An ovine model duplicating the surgical procedure for suburethral sling surgery was developed. Here we present the results after 3 months implantation using immunohistochemistry and advanced materials characterisation of two materials PP and Polyurethane (PU).</div><div>Both materials were well integrated into the tissue. The M1/M2 ratio in PP-implanted tissue was statistically significantly elevated (4.29) compared to PU (0.63) and control tissue (0.34). The higher ratio indicates a more inflammatory response to PP than PU.</div><div>Surface roughness (assessed using atomic force microscopy) increased in both materials, Rq from 5.73 to 10.2 nm in PP and from 1.03 to 2.96 nm in PU; whilst Ra went from 4.75 to 7.85 nm in PP and from 0.81 to 2.36 nm in PU. Notably, surface stiffness increased by 0.05 GPa in PP and decreased by 0.2 GPa in PU. PP underwent both surface and bulk material degradation, PU did not.</div><div>Detailed testing of implantable materials in an appropriate animal model should be conducted before materials are introduced into clinical practice. It is salutary that this has never been reported before. The use of material characterisation techniques allowed us to identify problems in the performance of PP, notably surface degradation, changes in bulk properties and stiffening, which can activate macrophages. In contrast, PU appears a more suitable alternative material for use in treating patients with SUI.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"328 ","pages":"Article 123852"},"PeriodicalIF":12.9,"publicationDate":"2025-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145538157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-13DOI: 10.1016/j.biomaterials.2025.123857
Yongzhao Su , Zhehao Wang , Qiuhui Hu , Yuji Sun , Qiuyang Dong , Ying Piao , Zheng Hua , Chengyuan Dong , Hongjie Hu , Youqing Shen , Zhuxian Zhou
Nanoparticle probes are advantageous over small molecular agents in medical imaging due to their prolonged circulation time, high payload capacity, and enhanced signal intensity. However, nanoparticle imaging probes are hindered by limited tissue penetration, often failing to provide sufficient contrast enhancement for imaging deep tissues. Herein, we developed a series of transcytosable iron oxide nanoparticles (TIONs) that penetrate deep tissue via cell transcytosis, enabling the T2-weighted magnetic resonance imaging (MRI) of the internal tissues. To fabricate TIONs, we prepared iron oxide nanoparticles (IONs) modified with in situ growth of polylysine dendrimers of third generation, terminated with different β-carboxylic amides. The capacity of these IONs to induce cell internalization, exocytosis, and transcytosis was evaluated using a fluorescence-based high-throughput screening assay. Among them, G3-FiA and G3-DiA TIONs exhibited efficient transcytosis capability towards cancer cells. We further demonstrated the feasibility and efficacy of these TIONs for deep MRI of 4T1 subcutaneous tumors, GL261 subcutaneous and orthotopic glioma tumors. Additionally, we identified TIONs as effective agents for kidney-targeted deep tissue imaging, highlighting the applicability of the strategy for non-tumor tissue imaging. This study offers critical insights for designing nanoparticle-based delivery systems with enhanced tissue penetration, thereby advancing their potential for deep-tissue imaging applications.
{"title":"Screening of transcytosable iron oxide nanoparticles (TIONs) for deep tissue-penetrating imaging","authors":"Yongzhao Su , Zhehao Wang , Qiuhui Hu , Yuji Sun , Qiuyang Dong , Ying Piao , Zheng Hua , Chengyuan Dong , Hongjie Hu , Youqing Shen , Zhuxian Zhou","doi":"10.1016/j.biomaterials.2025.123857","DOIUrl":"10.1016/j.biomaterials.2025.123857","url":null,"abstract":"<div><div>Nanoparticle probes are advantageous over small molecular agents in medical imaging due to their prolonged circulation time, high payload capacity, and enhanced signal intensity. However, nanoparticle imaging probes are hindered by limited tissue penetration, often failing to provide sufficient contrast enhancement for imaging deep tissues. Herein, we developed a series of transcytosable iron oxide nanoparticles (TIONs) that penetrate deep tissue via cell transcytosis, enabling the T<sub>2</sub>-weighted magnetic resonance imaging (MRI) of the internal tissues. To fabricate TIONs, we prepared iron oxide nanoparticles (IONs) modified with <em>in situ</em> growth of polylysine dendrimers of third generation, terminated with different β-carboxylic amides. The capacity of these IONs to induce cell internalization, exocytosis, and transcytosis was evaluated using a fluorescence-based high-throughput screening assay. Among them, G3-FiA and G3-DiA TIONs exhibited efficient transcytosis capability towards cancer cells. We further demonstrated the feasibility and efficacy of these TIONs for deep MRI of 4T1 subcutaneous tumors, GL261 subcutaneous and orthotopic glioma tumors. Additionally, we identified TIONs as effective agents for kidney-targeted deep tissue imaging, highlighting the applicability of the strategy for non-tumor tissue imaging. This study offers critical insights for designing nanoparticle-based delivery systems with enhanced tissue penetration, thereby advancing their potential for deep-tissue imaging applications.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"328 ","pages":"Article 123857"},"PeriodicalIF":12.9,"publicationDate":"2025-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145562070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The clinical utility of PEGylated nanomedicines is constrained by anti-polyethylene glycol (PEG) immunity, which drives accelerated blood clearance and infusion reactions. We address this by rationally tuning polymer architecture: a short-chain, high-density PEG brush (PEG500) grafted onto rigid mesoporous silica nanoparticles (MSNs). This design limits immune recognition through three synergistic features: (i) shortened PEG chains reduce epitope accessibility, (ii) high grafting density (4.43 chains/nm2) provides a strong steric barrier, and (iii) the rigid silica surface minimizes PEG backfolding/burial. At equivalent PEG concentrations, ELISAs revealed near-background binding of anti-PEG IgG (6.3) and IgM (AGP3) to MSN-PEG500, in sharp contrast to the strong recognition of PEG2000-based Lipodox. Antibody binding scaled with chain length (PEG2000 > PEG1000 > PEG500), consistent with increased epitope exposure on longer chains, while the compact PEG500 brush on MSNs largely abrogated detection. In vivo, radiolabeled MSN-PEG500 showed prolonged circulation and up to 25 %ID/g tumor uptake at 24 h. In robustly anti-PEG-immunized mice, doxorubicin-loaded MSN-PEG500 preserved antitumor efficacy with 100 % survival, whereas Lipodox (PEG2000) induced fatal hypersensitivity. Mechanistic studies implicated complement activation in PEG2000-associated immunotoxicity; C3 blockade with compstatin attenuated hypothermia (median ΔT reduced from ∼10 °C to ∼2 °C) in sensitized hosts. These findings indicate that nanoscale control of PEG conformation governs immune recognition and safety, offering a clinically tractable blueprint for engineering immune-evasive nanotherapeutics.
{"title":"Short-chain dense brush PEGylation on rigid nanocarriers overcomes anti-PEG antibody recognition for immune-stealth drug delivery","authors":"Ting-Yu Chen , Chi-Yuan Chang , Li Xu , Ting-Chun Wen , Yu-Han Lin , Cheng-Liang Peng , Yi-Qi Yeh , Chueh-Hsuan Chen , I-Lin Tsai , Kuo-Hsiang Chuang , Si-Han Wu","doi":"10.1016/j.biomaterials.2025.123854","DOIUrl":"10.1016/j.biomaterials.2025.123854","url":null,"abstract":"<div><div>The clinical utility of PEGylated nanomedicines is constrained by anti-polyethylene glycol (PEG) immunity, which drives accelerated blood clearance and infusion reactions. We address this by rationally tuning polymer architecture: a short-chain, high-density PEG brush (PEG500) grafted onto rigid mesoporous silica nanoparticles (MSNs). This design limits immune recognition through three synergistic features: (i) shortened PEG chains reduce epitope accessibility, (ii) high grafting density (4.43 chains/nm<sup>2</sup>) provides a strong steric barrier, and (iii) the rigid silica surface minimizes PEG backfolding/burial. At equivalent PEG concentrations, ELISAs revealed near-background binding of anti-PEG IgG (6.3) and IgM (AGP3) to MSN-PEG500, in sharp contrast to the strong recognition of PEG2000-based Lipodox. Antibody binding scaled with chain length (PEG2000 > PEG1000 > PEG500), consistent with increased epitope exposure on longer chains, while the compact PEG500 brush on MSNs largely abrogated detection. In vivo, radiolabeled MSN-PEG500 showed prolonged circulation and up to 25 %ID/g tumor uptake at 24 h. In robustly anti-PEG-immunized mice, doxorubicin-loaded MSN-PEG500 preserved antitumor efficacy with 100 % survival, whereas Lipodox (PEG2000) induced fatal hypersensitivity. Mechanistic studies implicated complement activation in PEG2000-associated immunotoxicity; C3 blockade with compstatin attenuated hypothermia (median ΔT reduced from ∼10 °C to ∼2 °C) in sensitized hosts. These findings indicate that nanoscale control of PEG conformation governs immune recognition and safety, offering a clinically tractable blueprint for engineering immune-evasive nanotherapeutics.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"328 ","pages":"Article 123854"},"PeriodicalIF":12.9,"publicationDate":"2025-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145562048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-12DOI: 10.1016/j.biomaterials.2025.123849
Qianqian Zhao , Yijing Zhao , Gaocheng Gai , Zhen Zhang , Bingbing Xie , Ankai Zheng , Manting Xie , Zitong Shen , Zhecun Wang , Yuansen Qin , Andy Peng Xiang , Ruiming Liu , Qiuling Xiang
Artificial blood vessels are commonly used to treat vascular damage and diseases. While large-diameter artificial blood vessels have been widely used in clinical practice, small-diameter artificial blood vessels (<6 mm) face significant challenges, including acute thrombosis in the short-term and intimal hyperplasia in the long-term. In this study, mesenchymal stem cells overexpressing Gremlin1 (Gremlin1-MSCs) were implanted as seed cells on the inner layer of polyurethane (PU) small-diameter artificial blood vessels fabricated by electrospinning technology. Additionally, the adhesion of Gremlin1-MSCs was enhanced by coating the lumen surface of the vessels with polydopamine (PDA). This approach aimed to promote endothelialization, thus reducing the risks of acute thrombosis and intimal hyperplasia. The in vitro results demonstrated that Gremlin1-MSCs retained their MSC characteristics and possessed the ability to inhibit monocyte proliferation and macrophage polarization. Furthermore, the PDA-coated PU small-diameter artificial blood vessels exhibited excellent biocompatibility and hemocompatibility. After implantation in rabbit carotid arteries, Gremlin1-MSCs significantly improved the long-term patency of small-diameter artificial blood vessels and reduced intimal hyperplasia of natural blood vessels at the suture site compared to the control MSCs (Ctrl-MSCs) or no MSCs treatment. This study provides a promising approach to improve the patency of small-diameter artificial blood vessels and highlights the potential of Gremlin1-MSCs as effective seed cells for this application.
人工血管通常用于治疗血管损伤和疾病。大直径人工血管已广泛应用于临床，而小直径人工血管(
{"title":"Gremlin1-MSCs seeded on small-diameter artificial blood vessels facilitate attenuation of post-transplantation intimal hyperplasia","authors":"Qianqian Zhao , Yijing Zhao , Gaocheng Gai , Zhen Zhang , Bingbing Xie , Ankai Zheng , Manting Xie , Zitong Shen , Zhecun Wang , Yuansen Qin , Andy Peng Xiang , Ruiming Liu , Qiuling Xiang","doi":"10.1016/j.biomaterials.2025.123849","DOIUrl":"10.1016/j.biomaterials.2025.123849","url":null,"abstract":"<div><div>Artificial blood vessels are commonly used to treat vascular damage and diseases. While large-diameter artificial blood vessels have been widely used in clinical practice, small-diameter artificial blood vessels (<6 mm) face significant challenges, including acute thrombosis in the short-term and intimal hyperplasia in the long-term. In this study, mesenchymal stem cells overexpressing Gremlin1 (Gremlin1-MSCs) were implanted as seed cells on the inner layer of polyurethane (PU) small-diameter artificial blood vessels fabricated by electrospinning technology. Additionally, the adhesion of Gremlin1-MSCs was enhanced by coating the lumen surface of the vessels with polydopamine (PDA). This approach aimed to promote endothelialization, thus reducing the risks of acute thrombosis and intimal hyperplasia. The in vitro results demonstrated that Gremlin1-MSCs retained their MSC characteristics and possessed the ability to inhibit monocyte proliferation and macrophage polarization. Furthermore, the PDA-coated PU small-diameter artificial blood vessels exhibited excellent biocompatibility and hemocompatibility. After implantation in rabbit carotid arteries, Gremlin1-MSCs significantly improved the long-term patency of small-diameter artificial blood vessels and reduced intimal hyperplasia of natural blood vessels at the suture site compared to the control MSCs (Ctrl-MSCs) or no MSCs treatment. This study provides a promising approach to improve the patency of small-diameter artificial blood vessels and highlights the potential of Gremlin1-MSCs as effective seed cells for this application.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"328 ","pages":"Article 123849"},"PeriodicalIF":12.9,"publicationDate":"2025-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145555957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-12DOI: 10.1016/j.biomaterials.2025.123855
Yubin Zhou , Xiangrong Pan , Tingting Hu , Weijie Tang , Zhenyu Xie , Mengyang Li , Yumeng Su , Zhan Zhou , Wenjie Feng , Hai Li , Meiting Zhao , Ruizheng Liang , Chaoliang Tan , Huizhi Chen
Sonodynamic therapy (SDT) has garnered significant attention due to its deep tissue penetration and biocompatibility. Nevertheless, the therapeutic outcomes of conventional SDT are substantially constrained by tumor hypoxia and the transient lifespan of reactive oxygen species (ROS). Herein, we report the nitric oxide (NO)-functionalized two-dimensional (2D) metal-organic framework (MOF) nanosheets as an efficient nanoplatform for ultrasound (US)-induced synergistic gas/sonodynamic cancer therapy. Zinc ions are initially chelated at the porphyrin center within the MOF framework (In-TCPP), followed by successive coordination and adsorption of NO gas to yield the NO-functionalized MOF (In-TCPP@Zn–NO). In comparison to pristine In-TCPP nanosheets, the US treatment can not only induce the efficient ROS generation but also trigger the efficient and rapid release of NO gas from the In-TCPP@Zn–NO nanosheets. Importantly, while maintaining comparable SDT efficacy to pristine In-TCPP nanosheets, the In-TCPP@Zn–NO demonstrates US-responsive NO release behavior with enhanced kinetics and distinct temporal regulation characteristics. Therefore, both in vitro and in vivo results demonstrate that US triggers the synergistic enhancement of SDT effectiveness by ROS generation and NO release from In-TCPP@Zn–NO nanosheets, leading to the eradication of cancer cells and tumors.
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