Pub Date : 1900-01-01DOI: 10.31073/vet_biotech35-01
M. Bogach, B. Stegniy, D. Bogach
{"title":"Morphological changes in the blood of sheep vaccinated against infective agalactia of sheep and goats","authors":"M. Bogach, B. Stegniy, D. Bogach","doi":"10.31073/vet_biotech35-01","DOIUrl":"https://doi.org/10.31073/vet_biotech35-01","url":null,"abstract":"","PeriodicalId":274594,"journal":{"name":"Bulletin \"Veterinary biotechnology\"","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133838686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.31073/vet_biotech41-03
A. M. Zhovnir, E.P. Mintciuk, O. Tarasov
{"title":"Application of multiplex PCR for the detection of toxin-producing Cl.\u0000 perfringens in biological samples","authors":"A. M. Zhovnir, E.P. Mintciuk, O. Tarasov","doi":"10.31073/vet_biotech41-03","DOIUrl":"https://doi.org/10.31073/vet_biotech41-03","url":null,"abstract":"","PeriodicalId":274594,"journal":{"name":"Bulletin \"Veterinary biotechnology\"","volume":"13 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120897519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.31073/vet_biotech39-09
I. Polupan, O. Rudoi, O. Lozhkina, V. G. Pavlunko, M. Kupnevska, N. I. Teplykh, A. L. Kravchenko, Y. Gibaliuk
Materials and methods of research. Pathological material (brain) was tested in regional laboratories of veterinary medicine by FAT. The presence of the biomarker tetracyclin was determined under a fluorescent microscope. Rabies antibody activity in blood serums were tested by ELISA. in 2018-2020 in Ukraine demonstrated the effectiveness of this measure, but to achieve the desired result, which will interrupt of the epizootic chain by forming of the high rabies population immunity (at least 50%), it is necessary campaigns of oral vaccination of wild carnivores against rabies twice a year - in spring and autumn.
{"title":"Assessment of the effectiveness of oral immunization of wild carnivorous\u0000 animals against rabies (2018-2020)","authors":"I. Polupan, O. Rudoi, O. Lozhkina, V. G. Pavlunko, M. Kupnevska, N. I. Teplykh, A. L. Kravchenko, Y. Gibaliuk","doi":"10.31073/vet_biotech39-09","DOIUrl":"https://doi.org/10.31073/vet_biotech39-09","url":null,"abstract":"Materials and methods of research. Pathological material (brain) was tested in regional laboratories of veterinary medicine by FAT. The presence of the biomarker tetracyclin was determined under a fluorescent microscope. Rabies antibody activity in blood serums were tested by ELISA. in 2018-2020 in Ukraine demonstrated the effectiveness of this measure, but to achieve the desired result, which will interrupt of the epizootic chain by forming of the high rabies population immunity (at least 50%), it is necessary campaigns of oral vaccination of wild carnivores against rabies twice a year - in spring and autumn.","PeriodicalId":274594,"journal":{"name":"Bulletin \"Veterinary biotechnology\"","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123769525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.31073/vet_biotech35-13
A. Pritychenko, P. Krasochko, A. N. Pritychenko, M. Ponaskov, N. M. Shagako, E. Veleva, N. Hudz
{"title":"Amino acid composition of the filtrate of the culture fluid of lactic acid bacteria","authors":"A. Pritychenko, P. Krasochko, A. N. Pritychenko, M. Ponaskov, N. M. Shagako, E. Veleva, N. Hudz","doi":"10.31073/vet_biotech35-13","DOIUrl":"https://doi.org/10.31073/vet_biotech35-13","url":null,"abstract":"","PeriodicalId":274594,"journal":{"name":"Bulletin \"Veterinary biotechnology\"","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129800459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.31073/vet_biotech35-15
M. Rudá, O. M. Vasjanovych, V. P. Sapeyko, Yu.A. Yanhol, Z. A. Levchenko, O. Kaminska
{"title":"Fusarioses among pigs","authors":"M. Rudá, O. M. Vasjanovych, V. P. Sapeyko, Yu.A. Yanhol, Z. A. Levchenko, O. Kaminska","doi":"10.31073/vet_biotech35-15","DOIUrl":"https://doi.org/10.31073/vet_biotech35-15","url":null,"abstract":"","PeriodicalId":274594,"journal":{"name":"Bulletin \"Veterinary biotechnology\"","volume":"183 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114397915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.31073/VET_BIOTECH34-10
P. Kryvoshyia, O. Rud
{"title":"Immunobiological indicators in horses with latent rhinopneumonia","authors":"P. Kryvoshyia, O. Rud","doi":"10.31073/VET_BIOTECH34-10","DOIUrl":"https://doi.org/10.31073/VET_BIOTECH34-10","url":null,"abstract":"","PeriodicalId":274594,"journal":{"name":"Bulletin \"Veterinary biotechnology\"","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126328553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.31073/vet_biotech39-04
O. Zakharova, O. Tarasov, Y. Yangol
{"title":"Use of polimerase chain reaction for detection of toxinproducing fungi of\u0000 Fusarium genus","authors":"O. Zakharova, O. Tarasov, Y. Yangol","doi":"10.31073/vet_biotech39-04","DOIUrl":"https://doi.org/10.31073/vet_biotech39-04","url":null,"abstract":"","PeriodicalId":274594,"journal":{"name":"Bulletin \"Veterinary biotechnology\"","volume":"189 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123083397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.31073/vet_biotech35-12
О. Nechyporenko, A. V. Berezovskyy, R. Petrov, А. І. Fotyn
{"title":"Research on species composition of microflora in poultry farms of different type","authors":"О. Nechyporenko, A. V. Berezovskyy, R. Petrov, А. І. Fotyn","doi":"10.31073/vet_biotech35-12","DOIUrl":"https://doi.org/10.31073/vet_biotech35-12","url":null,"abstract":"","PeriodicalId":274594,"journal":{"name":"Bulletin \"Veterinary biotechnology\"","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123487261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.31073/VET_BIOTECH38-14
E. A. Tsiganovich, S. Dybkova, E. A. Sirik, A. P. Golodyuk, V. A. Prokopenko, A. M. Zhovnir
SeNPs lead to increased production of antibodies and anti-inflammatory cytokines, respiratory activity of lymphocytes and peritoneal macrophages and survival of animals. The goal of the work was to determine the biosafety of promising SeNPs for their using in the biotechnology of veterinary immunological means. Materials and methods. SeNP synthesis performed by reducing selenic acid with ascorbic acid in the presence of polyvinylpyrrolidone. Three sols with SeNP with different selenium concentrations were made: № 1 – 0.05; № 2 – 0.5; № 3 – 0.25 mmol/l. The test object in evaluation toxic effects was the calf kidney cell culture (MDBK). SeNP cytotoxicity was assessed in vitro by cell viability with trypan blue dye. Genotoxicity estimation in vitro has been analyzed by the method of DNA comets in alkaline conditions. Results and discussions. Morphological characteristics of SeNPs determined by electron microscopy. It was found that in the sample № 1 spherical particles have a size of 20-80 nm, № 2 – 70-90 nm and № 3 – 80-90 nm. The results of cytotoxicity studies on test cells showed that at a dilution of 1:50 the survival rate of cells in samples №2 and №3 were 99%. Obtained results of genotoxicity studies showed that SeNP samples in dilution 1:50 were not genotoxic and may have a protective effect. Conclusions and prospects for further research. Studies performed to evaluate biosafety of SeNPs indicate the absence of cytotoxic and genotoxic effects on test cells. Therefore, the synthesized SeNPs are safe and biocompatible and can be used for manufacturing of experimental veterinary immunological means.
{"title":"Estimation of cytotoxicity and genotoxicity of selenium nanoparticles\u0000 stabilized with polyvinylpyrrolidone","authors":"E. A. Tsiganovich, S. Dybkova, E. A. Sirik, A. P. Golodyuk, V. A. Prokopenko, A. M. Zhovnir","doi":"10.31073/VET_BIOTECH38-14","DOIUrl":"https://doi.org/10.31073/VET_BIOTECH38-14","url":null,"abstract":"SeNPs lead to increased production of antibodies and anti-inflammatory cytokines, respiratory activity of lymphocytes and peritoneal macrophages and survival of animals. The goal of the work was to determine the biosafety of promising SeNPs for their using in the biotechnology of veterinary immunological means. Materials and methods. SeNP synthesis performed by reducing selenic acid with ascorbic acid in the presence of polyvinylpyrrolidone. Three sols with SeNP with different selenium concentrations were made: № 1 – 0.05; № 2 – 0.5; № 3 – 0.25 mmol/l. The test object in evaluation toxic effects was the calf kidney cell culture (MDBK). SeNP cytotoxicity was assessed in vitro by cell viability with trypan blue dye. Genotoxicity estimation in vitro has been analyzed by the method of DNA comets in alkaline conditions. Results and discussions. Morphological characteristics of SeNPs determined by electron microscopy. It was found that in the sample № 1 spherical particles have a size of 20-80 nm, № 2 – 70-90 nm and № 3 – 80-90 nm. The results of cytotoxicity studies on test cells showed that at a dilution of 1:50 the survival rate of cells in samples №2 and №3 were 99%. Obtained results of genotoxicity studies showed that SeNP samples in dilution 1:50 were not genotoxic and may have a protective effect. Conclusions and prospects for further research. Studies performed to evaluate biosafety of SeNPs indicate the absence of cytotoxic and genotoxic effects on test cells. Therefore, the synthesized SeNPs are safe and biocompatible and can be used for manufacturing of experimental veterinary immunological means.","PeriodicalId":274594,"journal":{"name":"Bulletin \"Veterinary biotechnology\"","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127176222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.31073/VET_BIOTECH34-20
Valeriu Еnciu
160 The future research will be focused of the effect of the chelate trace elements on the quality of chicken broilers meat under production conditions.
今后的研究重点是在生产条件下，螯合微量元素对肉鸡肉品质的影响。
{"title":"Incidence, diagnosis and treatment of some acropodium lesions in dairy cows in the conditions of farms of the Republic of Moldova","authors":"Valeriu Еnciu","doi":"10.31073/VET_BIOTECH34-20","DOIUrl":"https://doi.org/10.31073/VET_BIOTECH34-20","url":null,"abstract":"160 The future research will be focused of the effect of the chelate trace elements on the quality of chicken broilers meat under production conditions.","PeriodicalId":274594,"journal":{"name":"Bulletin \"Veterinary biotechnology\"","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127649004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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