{"title":"Biochemical and Molecular Characterization of Cell Wall Degrading Enzyme, Pectin Methylesterase Versus Banana Ripening: An Overview","authors":"C. Verma, A. Mani, Sanjay Mishra","doi":"10.3923/AJBKR.2017.1.23","DOIUrl":"https://doi.org/10.3923/AJBKR.2017.1.23","url":null,"abstract":"","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"69 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115061204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-12-15DOI: 10.3923/AJBKR.2017.24.34
P. Chandra, D. S. Arora
{"title":"Antioxidant Potential of Penicillium expansum and Purification of its Functional Compound","authors":"P. Chandra, D. S. Arora","doi":"10.3923/AJBKR.2017.24.34","DOIUrl":"https://doi.org/10.3923/AJBKR.2017.24.34","url":null,"abstract":"","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128624401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-12-15DOI: 10.3923/AJBKR.2017.35.42
J. Anerao, V. Jha, N. Desai
{"title":"Optimization of DNA Extraction Methods from Garcinia species for ISSR-PCR, RAPD-PCR and DNA Barcoding","authors":"J. Anerao, V. Jha, N. Desai","doi":"10.3923/AJBKR.2017.35.42","DOIUrl":"https://doi.org/10.3923/AJBKR.2017.35.42","url":null,"abstract":"","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114577961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Production of Antioxidant Bioactive Phenolic Compounds by Solid-state Fermentation on Agro-residues Using Various Fungi Isolated from Soil","authors":"P. Chandra, D. S. Arora","doi":"10.3923/AJBKR.2016.8.15","DOIUrl":"https://doi.org/10.3923/AJBKR.2016.8.15","url":null,"abstract":"","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117133184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-03-01DOI: 10.3923/AJBKR.2015.129.136
K. Devi, P. B. Sruthy, J. Anjana, J. Rathinamal
Plants produce a diverse range of bioactive compounds making them a rich source of different types of medicines. Ornamental plants are cultivated for adornment and to enhance the appearance of houses and also for commercial purposes. However, only very few of these ornamental plant species have found to be used in medicine and only little literature exit on their chemical and biological actions. In the present study, the evaluation of antimicrobial activities and identification of bioactive compounds using TLC and GC-MS of the A. columnaris bark extract were performed. In GC-MS bioactive compounds with medicinal value were identified, such as Benzoic acid, 1H-N-Hydroxynaphth (2,3) imidazole-6,7-dicarboximide, 2-Propenoic acid, 3-(4-methoxyphenyl), 1H-N-Hydroxynaphth (2,3-d) imidazole-6,7-dicarboximi. To prove the nontoxic nature of the plant, its crude bark extract was subjected to toxicity study using human embryonic kidney cell line. It reveal that the plant is minimal toxic to the human kidney cell line so usage of appropriate level will found to be safe and also carrying out some structural modification will help in the extraction of new drugs for pharmaceutical purpose.
植物产生各种各样的生物活性化合物，使它们成为不同类型药物的丰富来源。观赏植物的种植是为了装饰和提高房屋的外观，也有商业目的。然而，这些观赏植物中很少有药用价值，关于其化学和生物作用的文献也很少。本研究采用薄层色谱和气相色谱-质谱分析方法对竹节皮提取物进行了抗菌活性评价和活性成分鉴定。在GC-MS中鉴定出具有药用价值的生物活性化合物，如苯甲酸、1h - n -羟基(2,3)咪唑-6,7-二羰基苯胺、2-丙烯酸、3-(4-甲氧基苯基)、1h - n -羟基(2,3-d)咪唑-6,7-二羰基苯胺。为了证明该植物的无毒性质，对其粗树皮提取物进行了人体胚胎肾细胞系的毒性研究。结果表明，该植物对人体肾细胞系的毒性很小，适量使用是安全的，并对其结构进行修饰，有助于提取新的药用药物。
{"title":"Identification of Bioactive Compounds and Toxicity Study of Araucaria columnaris Bark Extract on Human Embryonic Kidney Cell Line","authors":"K. Devi, P. B. Sruthy, J. Anjana, J. Rathinamal","doi":"10.3923/AJBKR.2015.129.136","DOIUrl":"https://doi.org/10.3923/AJBKR.2015.129.136","url":null,"abstract":"Plants produce a diverse range of bioactive compounds making them a rich source of different types of medicines. Ornamental plants are cultivated for adornment and to enhance the appearance of houses and also for commercial purposes. However, only very few of these ornamental plant species have found to be used in medicine and only little literature exit on their chemical and biological actions. In the present study, the evaluation of antimicrobial activities and identification of bioactive compounds using TLC and GC-MS of the A. columnaris bark extract were performed. In GC-MS bioactive compounds with medicinal value were identified, such as Benzoic acid, 1H-N-Hydroxynaphth (2,3) imidazole-6,7-dicarboximide, 2-Propenoic acid, 3-(4-methoxyphenyl), 1H-N-Hydroxynaphth (2,3-d) imidazole-6,7-dicarboximi. To prove the nontoxic nature of the plant, its crude bark extract was subjected to toxicity study using human embryonic kidney cell line. It reveal that the plant is minimal toxic to the human kidney cell line so usage of appropriate level will found to be safe and also carrying out some structural modification will help in the extraction of new drugs for pharmaceutical purpose.","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"114 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131932193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-03-01DOI: 10.3923/AJBKR.2015.96.107
M. Al-Jailawi, H. Nas, Ghazi Munaim Azi
{"title":"Characterization of Biosurfactant Produced by a Novel Thermophillic Strain (Geobacillus thermoleovorans JQ 912239)","authors":"M. Al-Jailawi, H. Nas, Ghazi Munaim Azi","doi":"10.3923/AJBKR.2015.96.107","DOIUrl":"https://doi.org/10.3923/AJBKR.2015.96.107","url":null,"abstract":"","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"160 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132653762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-03-01DOI: 10.3923/AJBKR.2015.108.118
E. Gomaa
The γ-Amino Butyric Acid (GABA) is a major inhibitory neurotransmitter in central nervous system and its application in drugs and functional foods has attracted great attention. Most of the fermented food products are known and proven for its high content GABA producer, which makes the food product as potential functional foods. Lactic acid bacteria possess special physiological activities and are generally regarded as safe. This study was aimed at evaluating GABA production ability of some lactic acid bacteria strains isolated from Egyptian dairy products and further to increase the production by combining the best GABA producers. The impacts of pH, temperature, incubation time, initial monosodium glutamate (MSG) concentration and pyridoxal-5’-phosphate (PLP) addition on growth and γ-aminobutyric acid production were investigated. By optimizing these factors at pH5, cultivation temperature of 35°C, cultivation time of 72 h in medium supplemented with 750 M of MSG and 200 μM of PLP, Lactobacillus brevis NM101-1 and Lactobacillus plantarum DSM749 accumulated 168.58 and 140.69 mM of GABA, respectively. Co-culturing of both strains was not only able to enhance GABA production but also producing it in a short time (48 h), reached 224.69 mM. It could be concluded that the use of LAB strains in combination with a high GABA producer strain is a successful approach for reaching a physiologically active concentration of GABA.
{"title":"Enhancement of γ-Amminobutyric Acid Production by Co-Culturing of Two Lactobacilli Strains","authors":"E. Gomaa","doi":"10.3923/AJBKR.2015.108.118","DOIUrl":"https://doi.org/10.3923/AJBKR.2015.108.118","url":null,"abstract":"The γ-Amino Butyric Acid (GABA) is a major inhibitory neurotransmitter in central nervous system and its application in drugs and functional foods has attracted great attention. Most of the fermented food products are known and proven for its high content GABA producer, which makes the food product as potential functional foods. Lactic acid bacteria possess special physiological activities and are generally regarded as safe. This study was aimed at evaluating GABA production ability of some lactic acid bacteria strains isolated from Egyptian dairy products and further to increase the production by combining the best GABA producers. The impacts of pH, temperature, incubation time, initial monosodium glutamate (MSG) concentration and pyridoxal-5’-phosphate (PLP) addition on growth and γ-aminobutyric acid production were investigated. By optimizing these factors at pH5, cultivation temperature of 35°C, cultivation time of 72 h in medium supplemented with 750 M of MSG and 200 μM of PLP, Lactobacillus brevis NM101-1 and Lactobacillus plantarum DSM749 accumulated 168.58 and 140.69 mM of GABA, respectively. Co-culturing of both strains was not only able to enhance GABA production but also producing it in a short time (48 h), reached 224.69 mM. It could be concluded that the use of LAB strains in combination with a high GABA producer strain is a successful approach for reaching a physiologically active concentration of GABA.","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122992585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-02-01DOI: 10.3923/AJBKR.2015.46.59
S. L. Wee, P. Alderson, W. Yap
{"title":"Establishment of Plantlet Regeneration System from Nodal, Internodal and Leaf Explants of Sauropus androgynus (Sweet Shoot)","authors":"S. L. Wee, P. Alderson, W. Yap","doi":"10.3923/AJBKR.2015.46.59","DOIUrl":"https://doi.org/10.3923/AJBKR.2015.46.59","url":null,"abstract":"","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122436607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-02-01DOI: 10.3923/AJBKR.2015.80.87
S. Johari, S. Majumder
Banana bunchy top virus (BBVT) is the most threatening disease of banana all over the world. Control of BBTV involves the use of disease free planting material and prevention of infection. Its accurate detection is one of the functional approach to control it by eradication and exclusion. PCR is the most efficient mode of indexing but high-quality of DNA is one of the most essential factor for a successful amplification by PCR. In this study, we have developed a highly efficient low cost and rapid DNA extraction method for banana tissue which could yield good quantity of DNA free from protein and polysaccharide contamination. The DNA extracted by this method could be successfully amplified by PCR. In addition, the protocol could be completed within ~2 h.
{"title":"An Efficient DNA Extraction Protocol for Successful PCR Detection of Banana bunchy top virus from Banana Leaves","authors":"S. Johari, S. Majumder","doi":"10.3923/AJBKR.2015.80.87","DOIUrl":"https://doi.org/10.3923/AJBKR.2015.80.87","url":null,"abstract":"Banana bunchy top virus (BBVT) is the most threatening disease of banana all over the world. Control of BBTV involves the use of disease free planting material and prevention of infection. Its accurate detection is one of the functional approach to control it by eradication and exclusion. PCR is the most efficient mode of indexing but high-quality of DNA is one of the most essential factor for a successful amplification by PCR. In this study, we have developed a highly efficient low cost and rapid DNA extraction method for banana tissue which could yield good quantity of DNA free from protein and polysaccharide contamination. The DNA extracted by this method could be successfully amplified by PCR. In addition, the protocol could be completed within ~2 h.","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124674225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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