Pub Date : 2015-02-01DOI: 10.3923/AJBKR.2015.88.95
D. Krishna, T. Shasthree
An efficient procedure has been developed for induction of adventitious rooting and proliferation from different explants of Citrullus colocynthis. Leaf, stem, cotyledon and hypocotyledon explants cultured on different concentration of auxins and cytokinins, were supplemented on MS medium to test their ability to induce rhizogenesis. Murashige and Skoog (MS) medium fortified with 2,4-dichlorophenoxyacetic (2,4-D), indoleacetic acid (IAA), Indole-Butyric Acid (IBA) and Naphthalene Acetic Acid (NAA). Each at the concentrations of 0, 0.5, 1.0, 1.5, 2.0 and 2.5 mg LG were evaluated for their effects on adventitious root induction in the present study. High concentration of IAA (2.0 mg LG) and low concentration of IBA (1.5 mg LG) is best for induction of rhizogenesis. High concentration of IBA favored formation of malformed and thick roots. The NAA in combination with IBA is most potential in inducing high percentage of rooting (50%) and IAA in combination with IBA induced maximum rooting 62% with highest number of roots per shoot (3.80±0.2) and root length (3.8±0.1 cm) were obtained from leaf, cotyledon and stem than hypocotyls. Results revealed that, the best rooting ability was observed from in stem explants cultured on MS medium supplemented with 2.0 mg LG IAA and 1.5 mg LG IBA, multiple roots fibrous were produced from cotyledon explants on MS fortified with 2.0 mg LG 2,4-d and multiple roots cluster produced from leaf explants 1.5 mg LG IBA and 2.0 mg LG IAA. A distinct feature of this study is the adventitious rooting and proliferations from different explants of Citrullus colocynthis which has not been reported previously.
建立了一套有效的诱导西瓜不同外植体不定根和增殖的方法。在MS培养基上添加不同浓度生长素和细胞分裂素培养的叶、茎、子叶和下子叶外植体，检测其诱导生根的能力。添加2,4-二氯苯氧乙酸(2,4- d)、吲哚乙酸(IAA)、吲哚丁酸(IBA)和萘乙酸(NAA)的Murashige和Skoog (MS)培养基。本研究评价了0、0.5、1.0、1.5、2.0和2.5 mg LG对不定根诱导的影响。高浓度IAA (2.0 mg LG)和低浓度IBA (1.5 mg LG)对诱导生根效果最好。高浓度的IBA有利于形成畸形粗壮的根。NAA与IBA组合生根率最高(50%)，IAA与IBA组合生根率最高(62%)，叶片、子叶和茎的单根数(3.80±0.2)和根长(3.8±0.1 cm)均高于下胚轴。结果表明，茎外植体在添加2.0 mg LG IAA和1.5 mg LG IAA的MS培养基上生根能力最好，子叶外植体在添加2.0 mg LG 2,4-d的MS培养基上可形成多根纤维，叶片外植体在添加1.5 mg LG IAA和2.0 mg LG IAA的MS培养基上可形成多根簇。本研究的一个显著特点是西瓜不同外植体的不定根和增殖，这在以前的研究中没有报道过。
{"title":"Adventitious Rooting and Proliferation from Different Explants of Citrullus colocynthis (L.) Schrad an Endangered Medicinally Important Cucurbit","authors":"D. Krishna, T. Shasthree","doi":"10.3923/AJBKR.2015.88.95","DOIUrl":"https://doi.org/10.3923/AJBKR.2015.88.95","url":null,"abstract":"An efficient procedure has been developed for induction of adventitious rooting and proliferation from different explants of Citrullus colocynthis. Leaf, stem, cotyledon and hypocotyledon explants cultured on different concentration of auxins and cytokinins, were supplemented on MS medium to test their ability to induce rhizogenesis. Murashige and Skoog (MS) medium fortified with 2,4-dichlorophenoxyacetic (2,4-D), indoleacetic acid (IAA), Indole-Butyric Acid (IBA) and Naphthalene Acetic Acid (NAA). Each at the concentrations of 0, 0.5, 1.0, 1.5, 2.0 and 2.5 mg LG were evaluated for their effects on adventitious root induction in the present study. High concentration of IAA (2.0 mg LG) and low concentration of IBA (1.5 mg LG) is best for induction of rhizogenesis. High concentration of IBA favored formation of malformed and thick roots. The NAA in combination with IBA is most potential in inducing high percentage of rooting (50%) and IAA in combination with IBA induced maximum rooting 62% with highest number of roots per shoot (3.80±0.2) and root length (3.8±0.1 cm) were obtained from leaf, cotyledon and stem than hypocotyls. Results revealed that, the best rooting ability was observed from in stem explants cultured on MS medium supplemented with 2.0 mg LG IAA and 1.5 mg LG IBA, multiple roots fibrous were produced from cotyledon explants on MS fortified with 2.0 mg LG 2,4-d and multiple roots cluster produced from leaf explants 1.5 mg LG IBA and 2.0 mg LG IAA. A distinct feature of this study is the adventitious rooting and proliferations from different explants of Citrullus colocynthis which has not been reported previously.","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129916117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-02-01DOI: 10.3923/AJBKR.2015.60.70
K. Shivananda, S. Jagannatha, S. Lokesh, R. Vijayakuma, K. Mani
Studies have been undertaken to investigate, the effect of impeller number and its position upon the growth yield of Bordetella pertussis strain 509 during large scale batch cultivation. It was shown that, the maximum growth yield opacity was found to be 70 IOU mLG at 48 h cultivation in single impeller fixed at the bottom position of fermentor vessel. Whereas the optimal and moderate culture opacity 60 and 50 IOU mLG was observed during the two impellers mounted at two different positions on the shaft. Similarly the least growth yield was obtained during three impeller located at top, middle and bottom region of the shaft and the final opacity was found to be 30 IOU mLG at the end of 48 h cultivation with an increasing of culture pH 8.02. However, in two impeller combination experimental study also showed moderate growth yield at 48 h could give to homogenized mixing and good aeration. Thus, the two different location of the impeller combination influences two vortex flows, enhances optimal growth rate, viz; the lower impeller act as gas disperser and creates radial velocity with higher rates of air dispersion, whereas the upper impeller pumps the broth towards down thus the combined agitation of the broth leads to good mixing and even transfer of Dissolved Oxygen (DO2) to the growing cells and found moderate bacterial growth. In the combination of three impellers mounted at three different positions experimental study shown that, lesser growth yield at the end of 48 h cultivation, due to higher forth accumulation.
{"title":"Effect of Impeller Number and Position on Growth Yield and Virulence of Bordetella pertussis Strain 509 During Large Scale Batch Fermentor Cultivation","authors":"K. Shivananda, S. Jagannatha, S. Lokesh, R. Vijayakuma, K. Mani","doi":"10.3923/AJBKR.2015.60.70","DOIUrl":"https://doi.org/10.3923/AJBKR.2015.60.70","url":null,"abstract":"Studies have been undertaken to investigate, the effect of impeller number and its position upon the growth yield of Bordetella pertussis strain 509 during large scale batch cultivation. It was shown that, the maximum growth yield opacity was found to be 70 IOU mLG at 48 h cultivation in single impeller fixed at the bottom position of fermentor vessel. Whereas the optimal and moderate culture opacity 60 and 50 IOU mLG was observed during the two impellers mounted at two different positions on the shaft. Similarly the least growth yield was obtained during three impeller located at top, middle and bottom region of the shaft and the final opacity was found to be 30 IOU mLG at the end of 48 h cultivation with an increasing of culture pH 8.02. However, in two impeller combination experimental study also showed moderate growth yield at 48 h could give to homogenized mixing and good aeration. Thus, the two different location of the impeller combination influences two vortex flows, enhances optimal growth rate, viz; the lower impeller act as gas disperser and creates radial velocity with higher rates of air dispersion, whereas the upper impeller pumps the broth towards down thus the combined agitation of the broth leads to good mixing and even transfer of Dissolved Oxygen (DO2) to the growing cells and found moderate bacterial growth. In the combination of three impellers mounted at three different positions experimental study shown that, lesser growth yield at the end of 48 h cultivation, due to higher forth accumulation.","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123316978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-02-01DOI: 10.3923/AJBKR.2015.71.79
R. K. Negi, B. Joshi
Genetic based study provide emphasis on the population study of geographically isolated population, gene flow among them and evolutionary history of the species. In the present study, we aimed to detect the genetic variations in species of genus Schistura from Uttarakhand. Very little is known for the pattern of distribution and genetic diversity of the species of genus Schistura from the Garhwal and Kumaun region. Out of six primer only four primers were used to generate the fragments patterns from the samples collected. Polymorphism within and between the populations were assayed and total 60 bands were amplified ranging from 1-150 kb. Maximum number of bands was observed in Schistura montanus from the Srinagar, Garhwal and Uttarkashi regions. Total 60 bands were amplified in the all four primer with high percentage of polymorphic loci 50% Schistura montanus from Garhwal region and 28.8% in the S. montanus from the Kumaun region were observed. The level of heterozygosity were found 0.006-0.18 in the all three species. The percentage of polymorphism was 50% within the populations and high heterozygosity the sample of S. montanus when compared with other species. Observed pattern of RAPD markers reveals that the samples from the Garhwal region exhibit high diversity and used four primers are sufficient to distinguish the different population in the both region except the samples of the S. gangiticus of Garhwal region.
{"title":"Genetic Diversity between Populations of the Genus Schistura McClelland from the Garhwal and Kumaun Region Using RAPD Marker","authors":"R. K. Negi, B. Joshi","doi":"10.3923/AJBKR.2015.71.79","DOIUrl":"https://doi.org/10.3923/AJBKR.2015.71.79","url":null,"abstract":"Genetic based study provide emphasis on the population study of geographically isolated population, gene flow among them and evolutionary history of the species. In the present study, we aimed to detect the genetic variations in species of genus Schistura from Uttarakhand. Very little is known for the pattern of distribution and genetic diversity of the species of genus Schistura from the Garhwal and Kumaun region. Out of six primer only four primers were used to generate the fragments patterns from the samples collected. Polymorphism within and between the populations were assayed and total 60 bands were amplified ranging from 1-150 kb. Maximum number of bands was observed in Schistura montanus from the Srinagar, Garhwal and Uttarkashi regions. Total 60 bands were amplified in the all four primer with high percentage of polymorphic loci 50% Schistura montanus from Garhwal region and 28.8% in the S. montanus from the Kumaun region were observed. The level of heterozygosity were found 0.006-0.18 in the all three species. The percentage of polymorphism was 50% within the populations and high heterozygosity the sample of S. montanus when compared with other species. Observed pattern of RAPD markers reveals that the samples from the Garhwal region exhibit high diversity and used four primers are sufficient to distinguish the different population in the both region except the samples of the S. gangiticus of Garhwal region.","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"47 12","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132935190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-02-01DOI: 10.3923/AJBKR.2014.25.37
R. Hammond, J. Buah, P. A. Asare, S. Acheampong
{"title":"Optimizing Sterilization Condition for the Initiation of Sweet Potato (Ipomoea batatas) Culture in vitro","authors":"R. Hammond, J. Buah, P. A. Asare, S. Acheampong","doi":"10.3923/AJBKR.2014.25.37","DOIUrl":"https://doi.org/10.3923/AJBKR.2014.25.37","url":null,"abstract":"","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124660941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-02-01DOI: 10.3923/AJBKR.2013.51.60
C. Nwuche, H. Aoyagi, J. Ogbonna
{"title":"Citric acid production from cellulase-digested palm oil mill effluent.","authors":"C. Nwuche, H. Aoyagi, J. Ogbonna","doi":"10.3923/AJBKR.2013.51.60","DOIUrl":"https://doi.org/10.3923/AJBKR.2013.51.60","url":null,"abstract":"","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125253556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-02-01DOI: 10.3923/AJBKR.2013.33.50
R. Sebastian, Kim Jaeyoung, Kim Taehun, Lee Kyungtai
{"title":"Metagenomics: a promising approach to assess enzymes biocatalyst for biofuel production.","authors":"R. Sebastian, Kim Jaeyoung, Kim Taehun, Lee Kyungtai","doi":"10.3923/AJBKR.2013.33.50","DOIUrl":"https://doi.org/10.3923/AJBKR.2013.33.50","url":null,"abstract":"","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122604868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-03-01DOI: 10.3923/AJBKR.2012.129.136
K. R. Mahbub, A. Mohammad, M. Ahmed, S. Begum
{"title":"Decolorization of Synthetic Dyes Using Bacteria Isolated from Textile Industry Effluent","authors":"K. R. Mahbub, A. Mohammad, M. Ahmed, S. Begum","doi":"10.3923/AJBKR.2012.129.136","DOIUrl":"https://doi.org/10.3923/AJBKR.2012.129.136","url":null,"abstract":"","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2012-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130463837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparative Study of Microbial Fuel Cell for Electricity Generation by Enriched Exoelectron Generating Bacteria from Environmental Samples","authors":"Pratima Gupta, Piyush Parkhey, Komal Joshi, Anjali Mahilkar, Jasjeet Kaur Bhatia, L. Meena","doi":"10.3923/AJBKR.2012.137.142","DOIUrl":"https://doi.org/10.3923/AJBKR.2012.137.142","url":null,"abstract":"","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"144 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2012-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124458875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-03-01DOI: 10.3923/AJBKR.2012.120.128
A. Arumugam, K. Gopinath
{"title":"In vitro Micropropagation using Corm Bud Explants: An Endangered Medicinal Plant of Gloriosa superba L.","authors":"A. Arumugam, K. Gopinath","doi":"10.3923/AJBKR.2012.120.128","DOIUrl":"https://doi.org/10.3923/AJBKR.2012.120.128","url":null,"abstract":"","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2012-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116024932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-03-01DOI: 10.3923/AJBKR.2012.143.146
Prakasha, K. Rave, Kabballi Balakrishn, G. Lokesh
Plantlets regeneration through callus has been achieved in medicinal plant Aerva lanata of Amaranthaceae. Callus obtained by culturing young leaf disks on Murashige and Skoog's (MS) medium with 1.0 mg L-1 2, 4.D (dichlorophenoxyacetic acid) was subcultured on MS medium contg. 2.0 mg L-1 6, Benzyl Amino Purine (BAP), produced 30-40 multiple shoots. Rooting was achieved on half or full strength MS medium with 0.5-0.1 mg L-1 Naphthalene Acetic Acid (NAA) in 14 days. Regenerated plantlets were acclimatized and transferred to soil were showed normal morphol. characteristics. Survival of transplants was 90-95% under green house condition. [on SciFinder(R)]
苋菜科药用植物紫花苜蓿已经实现了愈伤组织再生。幼叶盘在含1.0 mg l - 1,2,4的Murashige和Skoog培养基上培养愈伤组织。二氯苯氧乙酸在MS培养基上传代培养。2.0 mg l - 16，苄基氨基嘌呤(BAP)，产生30-40个多枝。在含0.5 ~ 0.1 mg L-1萘乙酸(NAA)的半强或全强MS培养基上生根，培养时间为14 d。对再生植株进行驯化和移栽，使其形态恢复正常。特征。温室条件下移植物成活率为90-95%。(在SciFinder (R))
{"title":"Regeneration of Plantlets from Leaf Derived Callus of Aerva lanata, Juss: A Medicinal Plant","authors":"Prakasha, K. Rave, Kabballi Balakrishn, G. Lokesh","doi":"10.3923/AJBKR.2012.143.146","DOIUrl":"https://doi.org/10.3923/AJBKR.2012.143.146","url":null,"abstract":"Plantlets regeneration through callus has been achieved in medicinal plant Aerva lanata of Amaranthaceae. Callus obtained by culturing young leaf disks on Murashige and Skoog's (MS) medium with 1.0 mg L-1 2, 4.D (dichlorophenoxyacetic acid) was subcultured on MS medium contg. 2.0 mg L-1 6, Benzyl Amino Purine (BAP), produced 30-40 multiple shoots. Rooting was achieved on half or full strength MS medium with 0.5-0.1 mg L-1 Naphthalene Acetic Acid (NAA) in 14 days. Regenerated plantlets were acclimatized and transferred to soil were showed normal morphol. characteristics. Survival of transplants was 90-95% under green house condition. [on SciFinder(R)]","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2012-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132213022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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