Pub Date : 2011-04-01DOI: 10.3923/AJBKR.2011.317.328
R. Paliwal, Veena Sharma, Pracheta
{"title":"A Review on Horse Radish Tree (Moringa oleifera): A Multipurpose Tree with High Economic and Commercial Importance","authors":"R. Paliwal, Veena Sharma, Pracheta","doi":"10.3923/AJBKR.2011.317.328","DOIUrl":"https://doi.org/10.3923/AJBKR.2011.317.328","url":null,"abstract":"","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"108 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122541673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-04-01DOI: 10.3923/AJBKR.2011.357.367
N. N. Htwe, M. Maziah, H. Ling, F. Zaman, A. Zain
Rice, a salt-sensitive species has a substantial genetic variation for salt tolerance within the cultivated gene pool. This study describes the establishment of cell suspension culture system and analysis of proline content of the selected rice genotypes under in vitro salt stress. The cell suspension cultures, initiated from the friable seed-derived callus, were established in MS medium supplemented with different concentrations of 2,4-D (0-25 μM) and subjected to different concentrations of NaCl (0-250 mM). The average Settled Cell Volume (SCV) of different clumps sizes was recorded. The results showed that MR219-4 exhibited the highest cell growth rate and regeneration capacity. The 750 μm sieved clumps have higher cell mass than the 500 and 250 μm sieved cell size. The shoot-regeneration capacity was maximum in 1/2 MS medium supplemented with 10 μM BAP. However, low regeneration capacity was obtained in NaCl treatments. MR219 (control) and MR219-9 appeared to be more NaCl tolerant than MR219-4. In addition, all genotypes significantly synthesized proline under NaCl conditions as demonstrated by the proline accumulation and MR219-4 revealed the lowest content among genotypes studied. These genotypes can be good model systems for studying the physiological mechanisms associated with in vitro selection for salt stress. This finding also suggests that proline may play a crucial role in protecting rice cells under salinity stress.
{"title":"Regeneration capacity of cell suspension culture in Malaysian rice genotypes under salinity stress","authors":"N. N. Htwe, M. Maziah, H. Ling, F. Zaman, A. Zain","doi":"10.3923/AJBKR.2011.357.367","DOIUrl":"https://doi.org/10.3923/AJBKR.2011.357.367","url":null,"abstract":"Rice, a salt-sensitive species has a substantial genetic variation for salt tolerance within the cultivated gene pool. This study describes the establishment of cell suspension culture system and analysis of proline content of the selected rice genotypes under in vitro salt stress. The cell suspension cultures, initiated from the friable seed-derived callus, were established in MS medium supplemented with different concentrations of 2,4-D (0-25 μM) and subjected to different concentrations of NaCl (0-250 mM). The average Settled Cell Volume (SCV) of different clumps sizes was recorded. The results showed that MR219-4 exhibited the highest cell growth rate and regeneration capacity. The 750 μm sieved clumps have higher cell mass than the 500 and 250 μm sieved cell size. The shoot-regeneration capacity was maximum in 1/2 MS medium supplemented with 10 μM BAP. However, low regeneration capacity was obtained in NaCl treatments. MR219 (control) and MR219-9 appeared to be more NaCl tolerant than MR219-4. In addition, all genotypes significantly synthesized proline under NaCl conditions as demonstrated by the proline accumulation and MR219-4 revealed the lowest content among genotypes studied. These genotypes can be good model systems for studying the physiological mechanisms associated with in vitro selection for salt stress. This finding also suggests that proline may play a crucial role in protecting rice cells under salinity stress.","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131965640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-03-01DOI: 10.3923/AJBKR.2011.226.236
Saleh A. Al-Sohaiba
{"title":"Heavy Metal Tolerant Filamentous Fungi from Municipal Sewage for Bioleaching","authors":"Saleh A. Al-Sohaiba","doi":"10.3923/AJBKR.2011.226.236","DOIUrl":"https://doi.org/10.3923/AJBKR.2011.226.236","url":null,"abstract":"","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117253264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-03-01DOI: 10.3923/AJBKR.2011.246.253
K. Satyavani, T. Ramanathan, S. Gurudeeban
{"title":"Effect of Plant Growth Regulators on Callus Induction and Plantlet Regeneration of Bitter Apple (Citrullus colocynthis) from Stem Explant","authors":"K. Satyavani, T. Ramanathan, S. Gurudeeban","doi":"10.3923/AJBKR.2011.246.253","DOIUrl":"https://doi.org/10.3923/AJBKR.2011.246.253","url":null,"abstract":"","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"108 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115087386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-03-01DOI: 10.3923/AJBKR.2011.237.245
R. Kan, S. Vi
{"title":"Molecular Characterization of Antagonistic Streptomyces Isolated from a Mangrove Swamp","authors":"R. Kan, S. Vi","doi":"10.3923/AJBKR.2011.237.245","DOIUrl":"https://doi.org/10.3923/AJBKR.2011.237.245","url":null,"abstract":"","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"69 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129952115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-03-01DOI: 10.3923/AJBKR.2011.262.268
A. Al-Soqeer
{"title":"Genotypic diversity among wild populations of buffelgrass (Cenchrus ciliaris L. Link) in Al-Qassim region.","authors":"A. Al-Soqeer","doi":"10.3923/AJBKR.2011.262.268","DOIUrl":"https://doi.org/10.3923/AJBKR.2011.262.268","url":null,"abstract":"","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114765362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-03-01DOI: 10.3923/AJBKR.2011.214.225
R. Letsididi, T. Sun, W. Mu, N. H. Kessy, O. Djakpo, B. Jiang
{"title":"Production of a Thermoactive Β-cyclodextrin Glycosyltransferase with a High Starch Hydrolytic Activity from an Alkalitolerant Bacillus Licheniformis Sk 13.002 Strain","authors":"R. Letsididi, T. Sun, W. Mu, N. H. Kessy, O. Djakpo, B. Jiang","doi":"10.3923/AJBKR.2011.214.225","DOIUrl":"https://doi.org/10.3923/AJBKR.2011.214.225","url":null,"abstract":"","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"209 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116170917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-03-01DOI: 10.3923/AJBKR.2011.302.309
B. Y. Kamaruzzam, B. John, K. Zaleha, K. Jalal
An effort to assess the utility of 650 bp Cytochrome C oxidase subunit I (DNA barcode) gene in delineating the members horseshoe crabs (Family: xiphosura) with closely related sister taxa was made. A total of 33 sequences were extracted from National Center for Biotechnological Information (NCBI) which include horseshoe crabs (Class: Merostomata), beetles (Class: Insecta), common crabs (Class: Melacostraca) and scorpion (Class: Arachnida) sequences. Constructed phylogram through comprehensive dry lab methodology using advanced software predictive tools showed that beetles are closely related with horseshoe crabs than common crabs (Class: Melacostraca). It was interesting to note that terrestrial Scorpion (Class: Arachnida) were distantly related to horseshoe crabs (Class: Merostomata). Phylogram and observed Genetic Distance (GD) data were also revealed that Limulus polyphemus was distantly related to all the other horseshoe crab species. Tachypleus gigas was closely related with Carcinoscorpius rotundicauda than with Tachypleus tridentatus. The observed mean Genetic Distance (GD) value was higher in 3rd codon position in all the selected group of organisms. Among the horseshoe crabs high GC content was observed in L. polyphemus (38.32%) and lowest was observed in T. tridentatus (32.35%). We conclude that COI sequencing (barcoding) could be used in identifying and delineating evolutionary relatedness with closely related species
{"title":"Molecular phylogeny of horseshoe crab","authors":"B. Y. Kamaruzzam, B. John, K. Zaleha, K. Jalal","doi":"10.3923/AJBKR.2011.302.309","DOIUrl":"https://doi.org/10.3923/AJBKR.2011.302.309","url":null,"abstract":"An effort to assess the utility of 650 bp Cytochrome C oxidase subunit I (DNA barcode) gene in delineating the members horseshoe crabs (Family: xiphosura) with closely related sister taxa was made. A total of 33 sequences were extracted from National Center for Biotechnological Information (NCBI) which include horseshoe crabs (Class: Merostomata), beetles (Class: Insecta), common crabs (Class: Melacostraca) and scorpion (Class: Arachnida) sequences. Constructed phylogram through comprehensive dry lab methodology using advanced software predictive tools showed that beetles are closely related with horseshoe crabs than common crabs (Class: Melacostraca). It was interesting to note that terrestrial Scorpion (Class: Arachnida) were distantly related to horseshoe crabs (Class: Merostomata). Phylogram and observed Genetic Distance (GD) data were also revealed that Limulus polyphemus was distantly related to all the other horseshoe crab species. Tachypleus gigas was closely related with Carcinoscorpius rotundicauda than with Tachypleus tridentatus. The observed mean Genetic Distance (GD) value was higher in 3rd codon position in all the selected group of organisms. Among the horseshoe crabs high GC content was observed in L. polyphemus (38.32%) and lowest was observed in T. tridentatus (32.35%). We conclude that COI sequencing (barcoding) could be used in identifying and delineating evolutionary relatedness with closely related species","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"96 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133289119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-03-01DOI: 10.3923/AJBKR.2011.280.292
Priyanka Siwach, K. Grover, A. Gill
{"title":"The Influence of Plant Growth Regulators, Explant Nature and Sucrose Concentration on in vitro Callus Growth of Thevetia peruviana Schum","authors":"Priyanka Siwach, K. Grover, A. Gill","doi":"10.3923/AJBKR.2011.280.292","DOIUrl":"https://doi.org/10.3923/AJBKR.2011.280.292","url":null,"abstract":"","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"135 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116408773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-03-01DOI: 10.3923/AJBKR.2011.254.261
M. Somda, A. Savadogo, C. Ouattara, A. Ouattara, A. Traoré
{"title":"Improvement of bioethanol production using amylasic properties from Bacillus licheniformis and yeasts strains fermentation for biomass valorization.","authors":"M. Somda, A. Savadogo, C. Ouattara, A. Ouattara, A. Traoré","doi":"10.3923/AJBKR.2011.254.261","DOIUrl":"https://doi.org/10.3923/AJBKR.2011.254.261","url":null,"abstract":"","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127767006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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