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Antimicrobial resistance profile of bacterial pathogens isolated from naturally infected fish in some lakes of Ethiopia 从埃塞俄比亚一些湖泊中自然感染的鱼类中分离的细菌病原体的抗微生物药物耐药性谱
Pub Date : 2023-04-30 DOI: 10.4314/sinet.v46i1.4
Guta Dissasa, B. Lemma, H. Mamo
Small scale fisheries play important role in providing food and livelihoods for households in Ethiopia. However, prevalence of bacterial fish infection and their antimicrobial resistance are increasing over the world accounting for the major limitations in fish productionas well asits consumption. A cross sectional study was carried out to identify bacterial pathogens isolated from naturally infectedNile tilapia (Oreochromis niloticus) and 20 were common carp (Cyprinus carpio)fish showing clinical signs of disease particularly hemorrhage and skin ulceration and to determine the antibiotic susceptibility of fish associated bacteria in Ethiopia. A total of 42 fish samples from two lakes (Hawassa and Ziway) were aseptically collected and bacteria were isolated from the kidney, liver and intestine. The isolates were identified by their morphological characteristics, biochemical tests and sequencing of 16S rRNA genes. Nine well known fish pathogens were identified from 49 positive samples at the lakes with prevalence rate of Aeromonasveronii(21.43%), Proteusmirabilis (19.05%), Edwardsiella tarda (16.67%), Shigella flexneri(11.9%), Stenotrophomonas maltophilia (11.9%), Aeromonas hydrophila (11.9 %),Pseudomonas putida (9.5%), Aeromonassobria (7.14%) and Pseudomonas hunanensis (7.14%).Of these pathogens, six (6) mostprevalent andknown potential pathogens (A. hydrophila, A. veronii, E. tarda, P. mirabilis, S. flexneri and S. maltophilia)were evaluated for antibiotic susceptibility by using Kirby Bauer disk diffusion assay. All the isolates tested were resistant to at least three (3) of the eight antibiotics evaluated. High levels of resistance were expressed by the majority (87.5%) of the pathogens against penicillin and vancomycin. The results indicate the presence of potential pathogens andmaximum levels of acquired antibiotic resistance in fish bacteria from the study area. Thus, theuse of antibiotic in fish farming in Ethiopia should be discouraged.It isalso advisable to cook fish properly before consumption, in order to kill bacterialpathogens that may be present.
小规模渔业在为埃塞俄比亚家庭提供粮食和生计方面发挥着重要作用。然而,鱼类细菌感染的流行及其抗菌素耐药性在世界范围内正在增加,这是鱼类生产和消费的主要限制。开展了一项横断面研究,以确定从自然感染的尼罗罗非鱼(Oreochromis niloticus)和20种表现出疾病临床症状(特别是出血和皮肤溃疡)的普通鲤鱼(Cyprinus carpio)中分离出的细菌病原体,并确定埃塞俄比亚鱼类相关细菌的抗生素敏感性。在Hawassa湖和Ziway湖共采集42份鱼标本,对其肾脏、肝脏和肠道进行细菌分离。通过形态特征、生化检测和16S rRNA基因测序对分离菌株进行鉴定。49份阳性样本共检出9种常见病原菌,检出率分别为气单胞菌(21.43%)、奇异变形杆菌(19.05%)、迟发爱德华菌(16.67%)、福氏志贺氏菌(11.9%)、嗜麦芽窄养单胞菌(11.9%)、嗜水气单胞菌(11.9%)、恶臭假单胞菌(9.5%)、联合气单胞菌(7.14%)和湖南假单胞菌(7.14%)。采用Kirby Bauer纸片扩散法对6种最常见和已知的潜在致病菌(嗜水单胞菌、维罗氏单胞菌、迟缓单胞菌、奇异单胞菌、flexneri单胞菌和嗜麦芽单胞菌)进行药敏试验。所有被检测的分离株对所评估的8种抗生素中的至少3种具有耐药性。大多数病原菌(87.5%)对青霉素和万古霉素表现出高水平耐药。结果表明,研究区鱼类细菌中存在潜在病原体和获得性抗生素耐药性的最高水平。因此,埃塞俄比亚不应鼓励在养鱼业中使用抗生素。为了杀死可能存在的细菌病原体,在食用鱼之前适当煮熟也是明智的。
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引用次数: 0
Spatiotemporal changes in small mammal community of Wenchi highlands, central Ethiopia 埃塞俄比亚中部温奇高原小型哺乳动物群落的时空变化
Pub Date : 2023-04-30 DOI: 10.4314/sinet.v46i1.7
Kabeta Legese, A. Bekele
Small mammals show prominent spatial and temporal fluctuations. A study was conducted to assess the spatiotemporal dynamics of small mammals in Wenchi highlands, central Ethiopia. Sherman and snap traps were used to capture small mammals from four forests (Qibate, Lakeshore, Erica and Albesa), Hagenia woodland and Erica scrub in two dry and wet seasons between August 2019 and January 2021. A total of 959 small mammals represented by 12 rodent species (Arvicanthis abyssinicus, Desmomys yaldeni, Mastomys natalensis, M. awashensis, Stenocephaylemys albipes, Lophuromys flavopunctatus, L. brevicaudus, L. chrysopus, Dendromus lovati, Lophiomys imhausi, Graphirius murinus and an unidentified murid rodent), and 3 shrew species (Crocidura bailey, C. fumosa and C. olivieri) were recorded. Small mammals showed spatiotemporal variations. More individuals were trapped during the dry (58.29%) than the wet (41.71%) season. Lakeshore Forest yielded the highest number of small mammals, whereas Erica Forest contributed the least during both seasons. More small mammal individuals were trapped in the second (52.24%) than the first (47.75%) trapping year. Small mammals also showed significant variations between the trapping periods (P<0.05). High number of small mammals was trapped during the morning (84.98%) than evening traps. Seasonal differences in sexes and age groups were non-significant (P>0.05). The density of small mammals varied between seasons and habitats. Small mammals had higher density during the dry (83.33 ha-1) than the wet (59.35 ha-1) season. In conclusion, Wenchi highlands supported a diverse small mammal community that displayed spatiotemporal changes. As a result, priority should be given to conserve the area.
小型哺乳动物表现出明显的时空波动。对埃塞俄比亚中部温奇高原小型哺乳动物的时空动态进行了研究。在2019年8月至2021年1月的两个干湿季节,使用谢尔曼陷阱和捕捉器从四个森林(Qibate, lakesshore, Erica和Albesa), Hagenia林地和Erica灌丛中捕获小型哺乳动物。共记录到959种小型兽类,包括12种啮齿类(Arvicanthis abyssinicus, Desmomys yaldeni, Mastomys natalensis, M. awashensis, Stenocephaylemys albipes, Lophuromys flavopuncatus, L. brevicaudus, L. chrysopus, Dendromus lovati, Lophiomys imhausi, Graphirius murinus和一种未知的鼠)和3种鼩鼱(Crocidura bailey, C. fumosa和C. olivieri)。小型哺乳动物表现出时空差异。干季捕获个体数为58.29%,湿季捕获个体数为41.71%;湖滨森林的小型哺乳动物数量最多,而埃里卡森林在两个季节的贡献最少。第2年捕获的小兽个体数(52.24%)高于第1年(47.75%)。小型兽类在不同捕获期间也存在显著差异(P0.05)。小型哺乳动物的密度因季节和栖息地而异。干季小兽密度(83.33 ha-1)高于湿季(59.35 ha-1);综上所述,温池高原支持的小型哺乳动物群落多样性呈现出时空变化特征。因此,应该优先保护这一地区。
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引用次数: 2
Factors predicting knowledge sharing behavior of academic staff 学术人员知识共享行为的预测因素
Pub Date : 2023-04-30 DOI: 10.4314/sinet.v46i1.3
Gebremedhin Gebreyohans, David T. Croasdell, M. Meshesha
The main objective of this paper is to identify the factors that influence academic staff's knowledge-sharing intentions and behaviors in Ethiopian higher education. A structural equation model was used to validate the research framework using survey data from 256 respondents. The collected data has been analyzed using Smart PLS software. The result indicates that the factors that affect knowledge-sharing intention include social interaction, reciprocity, management support, reward systems, and reputation. Systems integrated into teaching-learning practice, availability of information technology infrastructure, perceived ease of use, and perceived usefulness are among the technological factors affecting the intention to use web technology for knowledge sharing toward knowledge-sharing behavior. It has also been concluded that management support had the greatest influence, but the reward system did not affect the knowledge-sharing intention. On the other hand, the availability of IT infrastructure has the largest effect on the intention to use web technology, while perceived ease of use has the least. The contributions of this study are twofold. The framework may serve as a roadmap for future researchers and managers considering their strategy to enhance knowledge sharing in HEI. The findings will benefit academic staff, university administrations, and higher education institutions. This study gives managers a solid foundation to promote and support academic staff's usage of web technology for knowledge sharing. The study will also help academic staff enhance their current knowledge-sharing practices.
本文的主要目的是确定影响埃塞俄比亚高等教育学术人员知识共享意愿和行为的因素。利用256名受访者的调查数据,采用结构方程模型来验证研究框架。使用Smart PLS软件对采集的数据进行分析。结果表明,影响知识共享意愿的因素包括社会互动、互惠、管理层支持、奖励制度和声誉。与教学实践相结合的系统、信息技术基础设施的可用性、感知易用性和感知有用性是影响使用网络技术进行知识共享意向的技术因素。研究还发现,管理层支持对知识共享意愿的影响最大,而奖励制度对知识共享意愿的影响较小。另一方面,IT基础设施的可用性对使用web技术的意向影响最大,而感知到的易用性影响最小。这项研究的贡献是双重的。该框架可以作为未来研究人员和管理人员考虑其加强高等教育知识共享战略的路线图。研究结果将有利于学术人员、大学管理部门和高等教育机构。本研究为管理者促进和支持学术人员使用网络技术进行知识共享提供了坚实的基础。这项研究还将帮助学术人员加强他们目前的知识共享实践。
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引用次数: 0
The Study of Interplay of Singlet Superconductivity and Ferromagnetism in Superconducting HoMo6Se8 超导体hom6se8中单线态超导性与铁磁性相互作用的研究
Pub Date : 2023-04-30 DOI: 10.4314/sinet.v46i1.5
Teshome Kebede Hambisa, Chernet Amente, Pooran Singh
This article reports the effect of magnetic ordering and superconducting order parameter on superconducting and magnetic transition temperatures of interacting singlet superconductivity and ferromagnetism in HoMo6Se8 material. The basic superconducting parameters were calculated starting with the BCS type model Hamiltonian and using the double time temperature dependent Green function formalisms. Results showed that the superconducting critical temperature decreases with enhancement of superconducting order parameter and vice versa. This is perhaps due to the weakening of the binding energy as the temperature approaches its critical value. On the other hand, the superconducting critical temperature demonstrates attenuation with increasing magnetic order parameters . It was also observed that the enhancement of magnetic transition temperature with the increase of the magnetic order parameter demonstrates variation with the alteration of the superconducting order parameter.  Moreover, the calculations revealed that there is a temperature region where both superconductivity and magnetic order coexist. Furthermore, the present theoretical analysis is broadly in agreement with existing experimental findings.
本文报道了磁有序和超导有序参数对hom6se8材料中单线态超导和铁磁性相互作用的超导和磁转变温度的影响。从BCS型模型哈密顿量出发,采用双时间温度相关格林函数形式计算了基本超导参数。结果表明,超导临界温度随着超导序参量的增大而降低,反之亦然。这可能是由于当温度接近其临界值时,结合能减弱。另一方面,超导临界温度随磁序参数的增加而衰减。磁转变温度随磁有序参数的增加而增强,随超导有序参数的改变而变化。此外,计算表明存在超导性和磁序共存的温度区域。此外,本文的理论分析与已有的实验结果基本一致。
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引用次数: 0
Soil Erosion Estimation Using Revised Universal Soil Loss Equation Integrated with Geographic Information System by Different Resolution Digital Elevation Model Data in Weyto Sub-Basin, Southern Ethiopia 埃塞俄比亚南部Weyto子流域不同分辨率数字高程模型数据与地理信息系统集成的修正通用水土流失方程土壤侵蚀估算
Pub Date : 2023-04-30 DOI: 10.4314/sinet.v46i1.1
Shimeles Damene, P. Satyal
Soil erosion is a global environmental challenge for developing countries including Ethiopia that require regular monitoring to take corrective measures. In this context, this study was focused on estimating soil erosion using the Revised Universal Soil Loss Equation (rusle) integrated with Geographical Information System (gis) technique for which it applied 30 m and 200 m resolution Digital Elevation Model (dem) data to generate slope gradient and length. Rainfall erosivity, soil erodibility, land cover/use and management factors data were obtained from existing studies and field-based assessments where the data were used to estimate the soil erosion using rusle model in ArcMap under two different dem resolution scenario. The model estimated an average of 1.38 and 1.86 million tons of annual soil loss by water using 200 and 30 meters resolution dem data, respectively, while keeping other factors constant. The erosion estimated using higher (30 m) resolution dem data was more realistic than low (200 m) resolution data , as the higher resolution dem data allowed less generalization. In high resolution dem data, the slopes generated were also more in line with ground reality. Based on the case study of Weyto sub-basin in Southern Ethiopia, we thus conclude that the gis technique and remote sensing data can be used in rusle based erosion risk prediction for large areas even at basin, sub-basin and macro watershed level. We suggest that the accuracy of the prediction can be improved by using high resolution (large scale) input data disaggregated by micro- and sub-watersheds.
土壤侵蚀是包括埃塞俄比亚在内的发展中国家面临的全球性环境挑战,需要定期监测以采取纠正措施。在此背景下,本研究的重点是利用经修订的通用土壤流失方程(rusle)和地理信息系统(gis)技术来估算土壤侵蚀,其中应用了30米和200米分辨率的数字高程模型(dem)数据来生成坡度和长度。降雨侵蚀力、土壤可蚀性、土地覆盖/利用和管理因子数据来源于已有研究和实地评估,并利用ArcMap中的rusle模型估算了两种不同dem分辨率情景下的土壤侵蚀。在保持其他因素不变的情况下,该模型分别使用200米和30米分辨率的dem数据估算年均水土流失量分别为138万吨和186万吨。使用高分辨率(30米)dem数据估算的侵蚀比低分辨率(200米)数据更真实,因为高分辨率dem数据的泛化程度较低。在高分辨率dem数据中,生成的坡度也更符合地面实际。以埃塞俄比亚南部Weyto子流域为例,分析了gis技术和遥感数据在流域、子流域和宏观流域层面上的应用前景。我们建议使用高分辨率(大尺度)的输入数据来分解微流域和次流域,可以提高预测的准确性。
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引用次数: 0
Mapping the soil thickness in the Main Ethiopian Rift using passive seismic data and HVSR, case for Northern water divided between the Ziway-Shala lakes basin and the Awash basin 利用被动地震数据和HVSR绘制埃塞俄比亚主要裂谷的土壤厚度图,以Ziway-Shala湖泊盆地和Awash盆地之间的北部水域为例
Pub Date : 2023-04-30 DOI: 10.4314/sinet.v46i1.2
Hailemichael Kebede
A passive seismic survey wasconducted in the northern water dividebetween the Ziway-Shala Lakes Basin, Central Main Ethiopian Rift (CMER), and Awash basin. Previous geophysical studies in the same areahad mainly focused on mapping intermediate to deeper Earth geological structures, and little attention given to shallowest layer.In contrast, the study aimed to map these shallow geologic structures of soil layer undulations at this specific location of CMER. A rapid and non-invasive technique was employed, which involve recording three component measurements of ambient seismic noise data. The collected seismic noise data was processed and analyzed using the average of Horizontal to Vertical Spectral Ratio (HVSR) which helpsto determine resonance frequencies of the survey stations. After frequency estimatesweremadefor each station, corresponding depth estimates were obtained using the method of Nakamura.The point depth to the shallowest layer was determined for each siteand these depth valueswere gridded and mapped to show subsurface topographic undulations or thickness of topsoil cover.The result showed that the shallowest bedrock layer wasthickening northwards, toward the Awash basin.
在Ziway-Shala湖泊盆地、埃塞俄比亚中部裂谷(CMER)和Awash盆地之间的北部水域进行了被动地震调查。以往在同一地区的地球物理研究主要集中在中深层地球地质构造的测绘上,对最浅层的研究很少。相比之下,该研究旨在绘制CMER特定位置的土层波动的浅层地质结构。采用了一种快速、无创的技术,记录环境地震噪声数据的三分量测量。对采集到的地震噪声数据进行处理和分析,利用水平与垂直频谱比(HVSR)平均值确定各测点的共振频率。在对每个台站进行频率估计后,利用Nakamura方法得到相应的深度估计。为每个站点确定到最浅层的点深度,这些深度值被网格化并映射,以显示地下地形波动或表土覆盖厚度。结果表明,最浅的基岩层向北加厚,朝向阿瓦什盆地方向。
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引用次数: 0
Population size and breeding ecology of Yellow-billed Duck (Anas undulata, Dubois 1839) in Chelekleka wetland, Ethiopia 埃塞俄比亚Chelekleka湿地黄嘴鸭(Anas undulata, Dubois 1839)种群规模和繁殖生态
Pub Date : 2023-04-30 DOI: 10.4314/sinet.v46i1.6
Mebrat Teklemariam Gebrekidan, A. Bekele, Bezawork Afework Bogale
Yellow-billed Duck (Anas undulata) is a monomorphic African endemic dabbling duck protected by the Conservation of African-Eurasian Migratory Water birds Agreement. This study was conducted to investigate the population size and breeding ecology of Yellow-billed Duck in Chelekleka wetland, Ethiopia from November 2020 to October 2021. Census was conducted during early morning from 6:30 - 10:00 hrs and late afternoon from 15:30 - 18:00 hrs twice per week when the birds are active. Direct observations as well as nest and egg morphometry measurements were conducted to collect data on breeding ecology of Yellow-billed Ducks. During the study period, eight active nests were identified and followed. Data were analysed using R version 4.2 software. A maximum of 315 individuals of Yellow-billed Ducks were counted, with a significantly high population size during dry season compared to wet season (Z = - 10.85, df = 63, p < 0.001). The female Yellow-billed Duck built a nest made up of herbs, defoliated leaves, dried grasses and thin twigs. The nest is circular shaped with a 26 - 32 cm in diameter and 7 - 11 cm in height. They laid eggs that are light brownish. Clutch size ranged from 5 - 8 eggs. The mean egg length, breadth and weight were 45.38 ± 1.65 mm, 39.17 ± 0.58 mm, and 45.4 ± 1.7 gm, respectively (n = 21). The average incubation period per nest was 19 ± 0.82 days (n = 4) with a high hatching success (98.1 %; n = 53). Supporting a considerable number of Yellow-billed Ducks, Chelekleka wetland is ecologically important for breeding and other related ecological requirements of the species. However, anthropogenic factors such as; burning of nesting bushes, wetland degradation, and agricultural expansions are highly threatened their survival. Therefore, sustainable conservation intervention is needed to minimize the threats on the species and its habitat.
黄嘴鸭(Anas undulata)是非洲特有的单态涉水鸭,受非洲-欧亚迁徙水鸟保护协定保护。本研究于2020年11月至2021年10月在埃塞俄比亚Chelekleka湿地对黄嘴鸭的种群规模和繁殖生态进行了调查。普查是在雀鸟活跃的时段,每星期进行两次,分别在清晨6时30分至10时及傍晚15时30分至18时进行。通过直接观察和巢、蛋形态测量,收集了黄嘴鸭繁殖生态的相关数据。在研究期间,发现并跟踪了8个活跃的巢穴。采用R 4.2软件对数据进行分析。黄嘴鸭种群数最多315只,枯水期黄嘴鸭种群数显著高于丰水期(Z = - 10.85, df = 63, p < 0.001)。母黄嘴鸭用草药、落叶、干草和细树枝筑了一个窝。巢为圆形,直径26 - 32厘米,高7 - 11厘米。它们下的蛋是浅褐色的。每窝5 - 8个蛋。平均卵长45.38±1.65 mm,卵宽39.17±0.58 mm,卵重45.4±1.7 gm (n = 21)。平均每窝孵化期为19±0.82天(n = 4),孵化成功率高达98.1%;N = 53)。Chelekleka湿地是相当数量的黄嘴鸭的栖息地,对该物种的繁殖和其他相关生态需求具有重要的生态意义。然而,人为因素如;巢丛焚烧、湿地退化和农业扩张都严重威胁着它们的生存。因此,需要采取可持续的保护措施,以尽量减少对该物种及其栖息地的威胁。
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引用次数: 0
Decomposition of BH-Lattices bh格的分解
Pub Date : 2023-04-30 DOI: 10.4314/sinet.v46i1.9
K. Venkateswarlu, Mekonnen Mamo Elema, Yibeltal Yitayew Tessema
 In this paper we study further properties of BH-lattices which is a subclass of BH-monoids. We furnish certain examples of BH- monoids which are not BH- lattice. We give a characterization of BH-lattices in terms of bounded BH-lattices and commutative l-groups. Also we prove that every BH-lattice is a direct product of Heyting algebra and commutative l-group under certain condition. Further we obtain the decomposition theorem in terms of Boolean algebra and a commutative l-group.
本文进一步研究了bh -格的性质,bh -格是bh -模群的一个子类。我们给出了一些非BH-晶格的BH- monoids的例子。用有界bh格和可交换l群给出了bh格的一个表征。并证明了在一定条件下,每一个bh格都是Heyting代数与可交换l群的直接乘积。进一步得到了布尔代数和交换l群的分解定理。
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引用次数: 0
Factors affecting cloud-based enterprise resource planning software adoption in Ethiopia 影响埃塞俄比亚采用云计算企业资源规划软件的因素
Pub Date : 2022-12-30 DOI: 10.4314/sinet.v45i3.5
Biniyam Fekadu, Lemma F. Lessa
Enterprise Resource Planning (erp) are integrated software solutions that transform organizations’ internal processes, provide collaboration with partners, external applications and information systems. Extant literature reveals that organizations are showing interest to transit from an on-premise erp to the new cloud-based erp solutions due to their extra benefits. There are few recent initiatives in Ethiopia to adopt cloud-based erp but challenges faced in the course of the adoption are not explored. Using the technology-organization-environment, diffusion of innovation, and the model of innovation resistance frameworks as lenses, this research aims at identifying factors contributing to the adoption of cloud-based erp in the Ethiopian context. A quantitative approach is adopted and survey was conducted using a self-administered online questionnaire using Google’s online form to gather data from employees of Ethiopian Shipping and Logistics Services Enterprise. Out of 295 questionnaires distributed, 152 valid questionnaires were collected and considered for the data analysis. The proposed model was tested using a partial least square with the help of the Smart pls software. The proposed model explained 58.5 % of the variance in cloud-based ERP adoption factors. The empirical analysis indicated that Relative advantage, Trust, IT Skill, and External pressure had a significant influence on the adoption of cloud-based erp in Ethiopia whereas Organizational Culture, Observability, and Trialability had no significant impact on the adoption of cloud erp service. The study provides a comprehensive understanding of the factors which affect the adoption of cloud-based erp technology in Ethiopia.
企业资源规划(erp)是集成的软件解决方案,可以改变组织的内部流程,提供与合作伙伴、外部应用程序和信息系统的协作。现有文献显示,由于其额外的好处,组织对从内部部署erp过渡到新的基于云的erp解决方案表现出兴趣。埃塞俄比亚最近很少有采用基于云的erp的举措,但没有探讨采用过程中面临的挑战。本研究以技术-组织-环境、创新扩散和创新阻力框架模型为视角,旨在确定在埃塞俄比亚背景下采用基于云的erp的因素。采用定量方法,使用谷歌的在线表格进行自我管理的在线问卷调查,以收集埃塞俄比亚航运和物流服务企业员工的数据。在发放的295份问卷中,收集到152份有效问卷进行数据分析。在Smart pls软件的帮助下,使用偏最小二乘法对所提出的模型进行了测试。提出的模型解释了基于云的ERP采用因素中58.5%的差异。实证分析表明,相对优势、信任、IT技能和外部压力对埃塞俄比亚云erp的采用有显著影响,而组织文化、可观察性和可试用性对云erp服务的采用没有显著影响。该研究提供了对影响埃塞俄比亚采用基于云的erp技术的因素的全面理解。
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引用次数: 0
DLLME coupled with HPLC-DAD for enrichment of pesticide residues in environmental sediment and soil samples DLLME联用HPLC-DAD富集环境沉积物和土壤样品中的农药残留
Pub Date : 2022-12-30 DOI: 10.4314/sinet.v45i3.2
Yosef Alemayehu, T. Tolcha, Negussie Megersa
Sample pre-treatment during determination of pesticides in sediment and soil is difficult due to matrix effects. For this reason, a low density dispersive liquid-liquid microextraction (ld-dllme) was developed for the determination of carbaryl, cynazine, atrazine, and propazine. The experimental parameters that could potentially influence performances of the developed analytical technique including the extraction solvent type and volume, disperser solvent type and volume, extraction and centrifugation time, centrifugation speed, salt concentration, and pH were optimized. The optimum experimental values were found to be 50 mL 1-octanol, 0.6 mL acetonitrile, 5 min extraction time, centrifugation at 3500 rpm for 3 min, 10% NaCl and pH 5. At the optimum conditions, the methods offer good linearity (R2 = 0.998–0.999) for the concentration ranges of 30-800 µg/kg; the detection limit (lod) ranging from 9-24 µg/kg; precision <5.3% rsd, and reproducibility 0.5-5.2% rsd. The accuracy of the method, determined in terms of recovery was found to vary from 74.5-109.7%. Therefore, the developed analytical method could be used for the determination of trace level of pesticides residues in sediment and agricultural soil samples.
在沉积物和土壤中农药的测定过程中,由于基质效应,样品预处理是困难的。为此,建立了一种低密度分散液-液微萃取法(ld-dllme),用于测定西威因、氰嗪、阿特拉津和丙嗪。对萃取溶剂类型和体积、分散剂类型和体积、萃取和离心时间、离心速度、盐浓度和pH等可能影响分析技术性能的实验参数进行了优化。最佳实验值为:1-辛醇50 mL,乙腈0.6 mL,提取时间5 min, 3500 rpm离心3 min, NaCl浓度10%,pH值5。在最佳条件下,该方法在30 ~ 800µg/kg范围内线性良好(R2 = 0.998 ~ 0.999);检出限(lod)范围为9-24µg/kg;精密度<5.3% rsd,重现性0.5-5.2% rsd。该方法在回收率方面的准确度为74.5-109.7%。因此,所建立的分析方法可用于沉积物和农业土壤样品中农药残留量的测定。
{"title":"DLLME coupled with HPLC-DAD for enrichment of pesticide residues in environmental sediment and soil samples","authors":"Yosef Alemayehu, T. Tolcha, Negussie Megersa","doi":"10.4314/sinet.v45i3.2","DOIUrl":"https://doi.org/10.4314/sinet.v45i3.2","url":null,"abstract":"Sample pre-treatment during determination of pesticides in sediment and soil is difficult due to matrix effects. For this reason, a low density dispersive liquid-liquid microextraction (ld-dllme) was developed for the determination of carbaryl, cynazine, atrazine, and propazine. The experimental parameters that could potentially influence performances of the developed analytical technique including the extraction solvent type and volume, disperser solvent type and volume, extraction and centrifugation time, centrifugation speed, salt concentration, and pH were optimized. The optimum experimental values were found to be 50 mL 1-octanol, 0.6 mL acetonitrile, 5 min extraction time, centrifugation at 3500 rpm for 3 min, 10% NaCl and pH 5. At the optimum conditions, the methods offer good linearity (R2 = 0.998–0.999) for the concentration ranges of 30-800 µg/kg; the detection limit (lod) ranging from 9-24 µg/kg; precision <5.3% rsd, and reproducibility 0.5-5.2% rsd. The accuracy of the method, determined in terms of recovery was found to vary from 74.5-109.7%. Therefore, the developed analytical method could be used for the determination of trace level of pesticides residues in sediment and agricultural soil samples.","PeriodicalId":275075,"journal":{"name":"SINET: Ethiopian Journal of Science","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129938790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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SINET: Ethiopian Journal of Science
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