Cytokine最新文献_第8页

Extracellular granzyme B and K are associated with endometriosis severity in infertile women 细胞外颗粒酶B和K与不孕妇女子宫内膜异位症的严重程度有关

IF 3.7 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Cytokine

Pub Date : 2025-12-01 Epub Date: 2025-10-04 DOI: 10.1016/j.cyto.2025.157045

Fadhil Ahsan , Budi Santoso , Nanda Yuli Rahmawati , Fidyah Nanda Alditia , Alfin Firasy Mufid , M.Y. Ardianta Widyanugraha , Ashon Sa'adi , Sri Ratna Dwiningsih , Arif Tunjungseto

Background

Endometriosis is a chronic inflammatory condition often associated with infertility. The role of granzymes as cytotoxic proteases and immune regulators in endometriosis remains poorly understood. This study evaluated serum and peritoneal fluid levels of granzymes A, B, and K in women with endometriosis and their links to disease severity.

Methodology

An observational study of 87 infertile women undergoing diagnostic laparoscopy, including 44 with endometriosis and 43 benign gynecologic controls. Granzyme levels were measured using ELISA and correlated with clinical parameters.

Results

Serum GrB and GrK were significantly higher in women with endometriosis, particularly in advanced stages. Peritoneal fluid revealed reduced GrA and increased GrB in the endometriosis group. Serum GrB and GrK levels correlated positively with rASRM adhesion and endometriosis severity scores, while GrB levels correlated inversely with the Endometriosis Infertility Index (EFI) score, indicating their role in predicting infertility. Combining serum GrB and GrK effectively differentiated endometriosis from control, highlighting their diagnostic potential.

Conclusion

Elevated serum GrB and GrK levels suggest their involvement in endometriosis pathogenesis and infertility. These findings highlight granzymes as promising biomarkers for disease severity, diagnosis, and targeted therapy in endometriosis management.

背景子宫内膜异位症是一种慢性炎症性疾病，通常与不孕症有关。颗粒酶作为细胞毒性蛋白酶和免疫调节剂在子宫内膜异位症中的作用仍然知之甚少。本研究评估了子宫内膜异位症患者血清和腹膜液颗粒酶A、B和K的水平及其与疾病严重程度的关系。方法对87例经腹腔镜诊断的不孕症患者进行观察性研究，其中44例为子宫内膜异位症，43例为良性妇科对照。采用酶联免疫吸附试验测定颗粒酶水平，并与临床参数相关。结果血清GrB和GrK在子宫内膜异位症患者中明显升高，尤其是晚期。腹膜液显示子宫内膜异位症组GrA降低，GrB升高。血清GrB和GrK水平与rASRM粘连和子宫内膜异位症严重程度评分呈正相关，而GrB水平与子宫内膜异位症不孕指数（EFI）评分呈负相关，提示其在预测不孕中的作用。结合血清GrB和GrK可有效区分子宫内膜异位症与对照组，突出其诊断潜力。结论血清GrB和GrK水平升高可能与子宫内膜异位症的发病和不孕有关。这些发现强调颗粒酶是子宫内膜异位症治疗中疾病严重程度、诊断和靶向治疗的有希望的生物标志物。

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引用次数: 0

First-trimester prediction of gestational diabetes mellitus using resistin, chemerin, progranulin, omentin, and IL-10 利用抵抗素、趋化素、颗粒前蛋白、大网膜蛋白和IL-10预测妊娠早期糖尿病。

IF 3.7 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Cytokine

Pub Date : 2025-12-01 Epub Date: 2025-10-16 DOI: 10.1016/j.cyto.2025.157057

Huriye Ezveci , Sukran Dogru , Fikriye Karanfil Yaman , Pelin Bahçeci , Hatice Bedia Yildiz , Meltem Uyaner Kan , İbrahim Kilinç , Kazım Gezginç

Objective

The study aims to evaluate the prediction of gestational diabetes mellitus (GDM) by proinflammatory resistin, chemerin, and progranulin, as well as anti-inflammatory adipokines omentin and IL-10, in maternal serum during the first trimester.

Material method

This prospective case-control study was performed in the obstetric unit of the university hospital from January 2023 to July 2024. Maternal serum samples from the study cases were collected during the first-trimester screening test to analyze resistin, chemerin, progranulin, omentin, and IL-10 levels. The cases were subsequently categorized into groups diagnosed with GDM and those not diagnosed between 24 and 28 weeks. Clinical and laboratory assessments were conducted.

Results

The study included a total of 160 pregnant women. 38 of these patients had GDM, and 122 had control cases. In the serum samples taken in the first trimester, resistin, chemerin, and IL-10 levels did not create a statistically significant difference in the GDM and control groups (p > 0.05). Statistical differences were observed between the groups in progranulin and omentin levels (p = 0.042, p = 0.004, respectively). For predicting GDM, the optimum cut-off value was 83.5 for progranulin (sensitivity: 57.9 %, specificity: 63.9 %, PPV: 33.3 %, NPV: 83 %) and 2.5 for omentin (sensitivity: 73.7 %, specificity: 58.2 %, PPV: 35.4 %, NPV: 87.7 %).

Conclusion

First-trimester progranulin and omentin levels may be useful in excluding GDM, but they lack sufficient power to diagnose it.

目的：探讨孕早期孕妇血清中促炎抵抗素、趋化素、前颗粒蛋白及抗炎脂肪因子大网膜蛋白、IL-10对妊娠期糖尿病（GDM）的预测作用。材料方法：本前瞻性病例对照研究于2023年1月至2024年7月在该大学附属医院产科进行。在妊娠早期筛查试验中收集孕妇血清样本，分析抵抗素、趋化素、颗粒前蛋白、大网膜蛋白和IL-10水平。这些病例随后被分为诊断为GDM的组和未诊断为24至28周的组。进行了临床和实验室评估。结果：该研究共包括160名孕妇。其中38例为GDM， 122例为对照。妊娠早期血清中抵抗素、趋化素、IL-10水平在GDM组与对照组间差异无统计学意义（p < 0.05）。各组间颗粒前蛋白、大网膜含量差异有统计学意义（p = 0.042, p = 0.004）。对于预测GDM，前颗粒蛋白的最佳临界值为83.5（敏感性：57.9%，特异性：63.9%,PPV: 33.3%, NPV: 83%），网膜蛋白的最佳临界值为2.5（敏感性：73.7%，特异性：58.2%,PPV: 35.4%, NPV: 87.7%）。结论：妊娠早期蛋白前和网膜蛋白水平可能有助于排除GDM，但它们缺乏足够的诊断能力。

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引用次数: 0

Inflammation in gastric epithelium associated with infection of Helicobacter pylori is induced by inhibited PPARγ pathway: the key role of TLRs/NF-κB axis 抑制PPARγ通路：TLRs/NF-κB轴的关键作用诱导幽门螺杆菌感染相关的胃上皮炎症。

IF 3.7 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Cytokine

Pub Date : 2025-12-01 Epub Date: 2025-10-22 DOI: 10.1016/j.cyto.2025.157061

Jianna Mao, Yuhou Shen, Hongyang Qi

Background

Helicobacter pylori infection is a major risk factor for the development of peptic ulcer disease and associated cancers, posing a significant public health concern. Peroxisome proliferator-activated receptor gamma (PPARγ) has been implicated in mucosal healing and may modulate H. pylori–induced gastric injury. In this study, we investigated the role of PPARγ in the progression of H. pylori–induced gastric ulceration.

Method

The potential association between PPARγ level and H. pylori infection rate was firstly analyzed with clinical samples. Then the effects of PPARγ activation on H. pylori–induced inflammation in human gastric epithelial cells (HGECs) were evaluated using CCK-8 assays for cell viability and immunofluorescence for inflammatory markers. The expression of PPARγ downstream effectors, including TLRs/NF-κB axis was also detected.

Results

Clinical analysis revealed that H. pylori infection significantly increased the risk of gastric ulcer development. PPARγ levels were inversely correlated with H. pylori infection rate and with the concentrations of IL-6, IL-1β, and TNF-α. In HGECs, lipopolysaccharide (LPS) treatment induced abnormal cell viability and heightened inflammatory responses, both of which were attenuated by the PPARγ agonist pioglitazone. Pioglitazone selectively promoted apoptosis in LPS-treated cells. At the molecular level, LPS increased expression of TLR2, TLR4, and TLR5, leading to NF-κB pathway activation; PPARγ activation suppressed both TLR expression and NF-κB signaling, coinciding with reduced cytokine release.

Conclusions

The current study demonstrates that PPARγ activation exerts anti–H. pylori effects during gastric ulcer progression by inhibiting the TLR/NF-κB signaling pathway.

背景：幽门螺杆菌感染是消化性溃疡疾病及相关癌症发展的主要危险因素，引起了重大的公共卫生关注。过氧化物酶体增殖物激活受体γ (PPARγ)与粘膜愈合有关，并可能调节幽门螺杆菌诱导的胃损伤。在这项研究中，我们研究了PPARγ在幽门螺杆菌引起的胃溃疡进展中的作用。方法：首先通过临床标本分析PPARγ水平与幽门螺杆菌感染率的潜在关系。然后利用CCK-8细胞活力和炎症标志物免疫荧光法评估PPARγ活化对幽门螺杆菌诱导的人胃上皮细胞（HGECs）炎症的影响。PPARγ下游效应因子，包括TLRs/NF-κB轴的表达也被检测。结果：临床分析显示幽门螺旋杆菌感染显著增加胃溃疡发生的风险。PPARγ水平与幽门螺杆菌感染率、IL-6、IL-1β、TNF-α浓度呈负相关。在hgec中，脂多糖（LPS）处理诱导细胞活力异常和炎症反应加剧，而PPARγ激动剂吡格列酮可减轻这两种反应。吡格列酮选择性促进lps处理的细胞凋亡。在分子水平上，LPS增加TLR2、TLR4、TLR5的表达，导致NF-κB通路活化；PPARγ激活抑制TLR表达和NF-κB信号，与细胞因子释放减少一致。结论：目前的研究表明，PPARγ激活具有抗h作用。通过抑制TLR/NF-κB信号通路抑制幽门螺杆菌在胃溃疡进展中的作用。

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引用次数: 0

Sepsis-induced cardiac dysfunction: Gender bias role of allograft inflammatory factor-1 败血症引起的心功能障碍：异体移植物炎症因子-1的性别偏见作用

IF 3.7 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Cytokine

Pub Date : 2025-12-01 Epub Date: 2025-10-09 DOI: 10.1016/j.cyto.2025.157040

B.C. Wang , S. Chaki , H. Taufiq , K. Sikder , S.K. Shukla , K. Rafiq

Sepsis-induced cardiac dysfunction is a major contributor to the high morbidity and mortality rates seen in septic patients, with those suffering from concurrent cardiac dysfunction experiencing mortality rates up to 90 %. This study elucidates the role of allograft inflammatory factor-1 (AIF-1) in sepsis-induced cardiac dysfunction, using a cecal ligation and puncture (CLP) model in both wild-type (WT) and AIF-1 knockout (^-/-) C57BL/6 mice. We specifically examined AIF-1's expression across genders and its impact on cardiac function and inflammation.

Results indicate a significant gender-specific upregulation of AIF-1 in female murine hearts during septic shock, suggesting a protective role of this molecule in mitigating cardiac dysfunction. In contrast, AIF-1 knockout female mice exhibited exacerbated cardiac dysfunction compared to their wild-type counterparts, as evidenced by decreased ejection fraction and fractional shortening, increased expression of pro-inflammatory cytokines (TNF-α, IL-6), and heightened cardiac inflammation. Moreover, AIF-1 deletion reduced macrophage infiltration in the heart, underscoring its role in modulating immune responses during septic challenges.

These findings highlight the protective effects of AIF-1 in female mice and suggest its potential as a therapeutic target for sepsis-induced cardiac dysfunction. Understanding AIF-1's mechanisms may facilitate the development of gender-specific treatments to improve outcomes in sepsis, particularly by leveraging its role in enhancing cardiac resilience and modulating inflammation.

脓毒症引起的心功能障碍是导致脓毒症患者高发病率和高死亡率的主要原因，并发心功能障碍的患者死亡率高达90%。本研究通过野生型（WT）和AIF-1敲除（-/-）C57BL/6小鼠的盲肠结扎穿刺（CLP）模型，阐明了同种异体移植物炎症因子-1 （AIF-1）在败血症诱导的心功能障碍中的作用。我们专门研究了AIF-1在性别中的表达及其对心功能和炎症的影响。结果表明，在感染性休克期间，雌性小鼠心脏中AIF-1有显著的性别特异性上调，表明该分子在减轻心功能障碍方面具有保护作用。相比之下，与野生型小鼠相比，AIF-1敲除的雌性小鼠表现出更严重的心功能障碍，这可以通过射血分数和分数缩短降低、促炎细胞因子（TNF-α、IL-6）表达增加和心脏炎症加剧来证明。此外，AIF-1缺失减少了心脏中巨噬细胞的浸润，强调了其在脓毒性挑战中调节免疫反应的作用。这些发现强调了AIF-1对雌性小鼠的保护作用，并提示其可能作为脓毒症引起的心功能障碍的治疗靶点。了解AIF-1的机制可以促进性别特异性治疗的发展，以改善败血症的预后，特别是通过利用其在增强心脏恢复力和调节炎症中的作用。

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引用次数: 0

Differential cytokine and chemokine signatures in bronchoalveolar lavage and plasma predict clinical outcomes of COVID-19 patients hospitalized in intensive care unit 支气管肺泡灌洗液和血浆中细胞因子和趋化因子的差异特征预测重症监护病房住院患者的临床结局。

IF 3.7 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Cytokine

Pub Date : 2025-12-01 Epub Date: 2025-10-01 DOI: 10.1016/j.cyto.2025.157042

Antonio Piralla , Sabrina Gioria , Giuditta Guerrini , Greta Petazzoni , Guglielmo Ferrari , Alessandro Ferrari , Federica Giardina , Federica Bergami , Anita Orlando , Silvia Mongodi , Federico Capra Marzani , Mara De Amici , Giorgia Testa , Luigi Calzolai , Fausto Baldanti

This study explores the immune response dynamics in critically ill SARS-CoV-2 patients compared to non-SARS-CoV-2 controls both hospitalized in intensive care unit (ICU), focusing on localized and systemic inflammatory profiles to identify patterns linked to clinical outcomes. A cohort of ICU patients (37 SARS-CoV-2 positive, 17 controls) underwent bronchoalveolar lavage (BAL) and plasma analysis to assess inflammatory markers using a multiplex assay. SARS-CoV-2 patients showed distinct clinical and immunological features, including prolonged ICU stays and elevated markers of systemic inflammation. Cluster analysis revealed subgroup-specific immune signatures, with COVID-19 survivors demonstrating coordinated cytokine/chemokine interactions suggestive of adaptive immune regulation, while non-survivors exhibited dysregulated profiles indicative of maladaptive inflammation. The study underscores the spatial segregation of immune responses, with lung-specific inflammation in BAL contrasting with systemic profiles, highlighting the importance of tissue-targeted monitoring. These findings suggest that immune profiling could inform stratification for tailored immunomodulatory therapies, advancing precision approaches in critical care for severe SARS-CoV-2 infections.

本研究探讨重症监护病房（ICU）重症SARS-CoV-2患者与非SARS-CoV-2对照组的免疫反应动态，重点关注局部和全身炎症特征，以确定与临床结果相关的模式。一组ICU患者（37例SARS-CoV-2阳性，17例对照组）接受了支气管肺泡灌洗（BAL）和血浆分析，使用多重测定法评估炎症标志物。SARS-CoV-2患者表现出明显的临床和免疫学特征，包括ICU住院时间延长和全身炎症指标升高。聚类分析揭示了亚群特异性免疫特征，COVID-19幸存者表现出协调的细胞因子/趋化因子相互作用，表明适应性免疫调节，而非幸存者表现出失调的特征，表明适应性炎症不良。该研究强调了免疫反应的空间分离，BAL中的肺部特异性炎症与全身情况形成对比，强调了组织靶向监测的重要性。这些发现表明，免疫分析可以为量身定制的免疫调节疗法分层提供信息，从而推进重症SARS-CoV-2感染的精准治疗方法。

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引用次数: 0

The significance of serum IL-18 levels in Hodgkin lymphoma patients 霍奇金淋巴瘤患者血清IL-18水平的意义

IF 3.7 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Cytokine

Pub Date : 2025-12-01 Epub Date: 2025-10-03 DOI: 10.1016/j.cyto.2025.157044

Gülden Sincan , Suat Sincan , Çağrı Kızıltunç , Esra Eğilmez

Background

Inflammation plays a crucial role in the pathogenesis of Hodgkin lymphoma. IL-18 is a proinflammatory cytokine. Therefore, we investigated the serum IL-18 levels and their prognostic significance in Hodgkin lymphoma patients.

Method

Serum IL-18 levels were compared between 30 newly diagnosed Hodgkin lymphoma patients and 30 healthy controls. The association between IL-18 levels, clinical findings, prognostic markers, and treatment response was also evaluated in Hodgkin lymphoma patients.

Results

The HL group's IL-18 level (29.28 ± 13.48 pg/mL) was significantly higher than that of the control group (16.69 ± 9.33 pg/mL) (p < 0.001). Significant associations were observed between IL-18 levels and extranodal involvement, B symptom presence, performance status, Ann-Arbor stage, bulky mass presence, bone marrow involvement, and treatment response in Hodgkin lymphoma patients (p = 0.013, p = 0.006, p = 0.009, p = 0.003, p = 0.001, p = 0.02, p = 0.03, respectively). IL-18 levels also positively correlated with LDH and beta-2 microglobulin levels (r = 0.37, p = 0.04, r = 0.5, p = 0.005, respectively).

Conclusion

Serum IL-18 levels are higher in Hodgkin lymphoma patients compared to healthy individuals. IL-18 levels may serve as a prognostic marker and a predictor of treatment response in Hodgkin lymphoma patients.

背景：炎症在霍奇金淋巴瘤的发病机制中起着至关重要的作用。IL-18是一种促炎细胞因子。因此，我们研究了霍奇金淋巴瘤患者血清IL-18水平及其预后意义。方法比较30例新诊断霍奇金淋巴瘤患者与30例健康对照者血清IL-18水平。在霍奇金淋巴瘤患者中，还评估了IL-18水平、临床表现、预后指标和治疗反应之间的关系。结果HL组IL-18水平（29.28±13.48 pg/mL）显著高于对照组（16.69±9.33 pg/mL）（p < 0.001）。在霍奇金淋巴瘤患者中，IL-18水平与结外受损伤、B症状存在、运动状态、an - arbor分期、大块肿块存在、骨髓受损伤和治疗反应之间存在显著相关性（p = 0.013, p = 0.006, p = 0.009, p = 0.003, p = 0.001, p = 0.02, p = 0.03）。IL-18水平与LDH和β -2微球蛋白水平呈正相关（r = 0.37, p = 0.04, r = 0.5, p = 0.005）。结论霍奇金淋巴瘤患者血清IL-18水平高于健康人群。IL-18水平可作为霍奇金淋巴瘤患者治疗反应的预后标志物和预测因子。

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引用次数: 0

The relationship of plasma netrin-1 level with disease activity and other parameters in patients with axial spondyloarthritis 轴型脊柱炎患者血浆netrin-1水平与疾病活动度等参数的关系

IF 3.7 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Cytokine

Pub Date : 2025-12-01 Epub Date: 2025-10-14 DOI: 10.1016/j.cyto.2025.157055

Fatih Serdar Baykal , Serdar Can Güven , Ahmet Kor , Funda Eren , Salim Neşelioğlu , Bünyamin Polat , Serdar Esmer , Berkan Armağan , Kevser Orhan , İsmail Doğan , Yüksel Maraş , Salih Başer , Özcan Erel , Şükran Erten

Aim of this study was to evaluate plasma netrin-1 levels in axial spondyloarthritis and to investigate relations between disease activity and other parameters. Axial spondyloarthritis is a term defining a form of the spondyloarthritis in which axial skeleton is predominantly affected. Netrin-1 is a laminin-like matrix protein belonging to the axonal guide protein family, controlling neuronal migration in the embryonic period and inhibiting leukocyte aggregation to provide neuronal protection in the tissue. Study was conducted cross-sectional. Patients with axial spondyloarthritis between ages of 18–65 who applied to our outpatient clinic were enrolled upon consent. A control group was formed by healthy volunteers. Demographics, clinic, laboratory, imaging data and disease activity scores were recorded. Spondyloarthritis patients were subgrouped as ankylosing spondylitis and non-radiographic axial spondyloarthritis. Serum netrin-1 was measured by a commercial kit in patient and control groups. A total of 60 spondyloarthritis patients and 56 healthy controls were enrolled. No significant differences in serum netrin-1 levels were observed among patient groups and controls. Netrin-1 levels had a significant positive correlation with disease activity parameters and found to be higher in patients with higher disease activity. Receiver operating curve analyses revealed fair discriminative power for active disease. Our results suggest a utility for netrin-1 as a novel biomarker for detecting disease activity in spondyloarthritis, for the first time to the best of our knowledge.

本研究的目的是评估轴型脊柱炎患者血浆netrin-1水平，并探讨疾病活动性与其他参数的关系。轴向脊柱炎是一个术语，定义了一种形式的脊柱炎，其中轴向骨骼主要受到影响。Netrin-1是一种层粘连蛋白样基质蛋白，属于轴突引导蛋白家族，在胚胎期控制神经元迁移，抑制白细胞聚集，在组织中提供神经元保护。研究是横断面进行的。年龄在18-65岁之间的轴性脊柱炎患者经同意进入我们的门诊。对照组由健康志愿者组成。记录人口统计学、临床、实验室、影像学数据和疾病活动评分。脊柱炎患者分为强直性脊柱炎和非放射性轴性脊柱炎。患者和对照组的血清netrin-1用商用试剂盒测定。共有60名脊椎关节炎患者和56名健康对照者被纳入研究。患者组与对照组血清netrin-1水平无显著差异。Netrin-1水平与疾病活动性参数呈显著正相关，疾病活动性越高，Netrin-1水平越高。受试者工作曲线分析显示，活动性疾病的鉴别能力尚可。我们的研究结果表明，据我们所知，netrin-1是一种新的生物标志物，可用于检测脊椎关节炎的疾病活动性。

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引用次数: 0

IL-6 and TNF variants as potential determinants of perianal disease in Crohn's patients: a pilot study IL-6和TNF变异是克罗恩病患者肛周疾病的潜在决定因素：一项初步研究

IF 3.7 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Cytokine

Pub Date : 2025-12-01 Epub Date: 2025-11-01 DOI: 10.1016/j.cyto.2025.157064

Jessica Cusato , Gian Paolo Caviglia , Alfredo Santovito , Gabriele Ascani , Alessandra Manca , Marta Vernero , Angelo Armandi , Eleonora Dileo , Miriam Antonucci , Maria Alessandra Pavan , Antonio D'Avolio , Davide Giuseppe Ribaldone

Perianal Crohn's disease (pCD) is a debilitating complication of Crohn's disease (CD), affecting approximately 20 % of patients. Genetic polymorphisms in cytokine-related and intracellular signaling pathway genes may influence pCD development, yet data on their role remain limited.

To evaluate the association between specific single nucleotide polymorphisms (SNPs) in cytokine (TNF, IL-6, IL-10, TGF-β) and MAPK pathway-related genes (RAS, RAF-1, ERK) and the pCD development.

This retrospective study included 204 CD patients from a single center, of whom 34 had documented pCD. Clinical, phenotypic, and treatment data were collected. Genotyping was performed by real-time PCR and ARMS-PCR.

IL-6 -597 GG genotype was significantly associated with a reduced risk of pCD (p = 0.049). TNF rs1800629–308 GA genotype showed a trend toward protection (p = 0.07). IL-10 -1082 AA genotype showed a non-significant trend toward increased risk (p = 0.07). No significant associations were found for TGF-β or MAPK-related polymorphisms.

Our findings suggest that polymorphisms in cytokine genes, particularly IL-6 and TNF, may influence the risk of developing perianal complications in CD. These preliminary results warrant validation in larger cohorts to assess their potential as predictive biomarkers for personalized management of pCD.

肛周克罗恩病（pCD）是克罗恩病（CD）的一种使人衰弱的并发症，约影响20%的患者。细胞因子相关基因和细胞内信号通路基因的遗传多态性可能影响pCD的发展，但其作用的数据仍然有限。评估细胞因子（TNF、IL-6、IL-10、TGF-β）和MAPK通路相关基因（RAS、RAF-1、ERK）特异性单核苷酸多态性（snp）与pCD发生的关系。这项回顾性研究包括来自单一中心的204例CD患者，其中34例记录为pCD。收集临床、表型和治疗数据。采用实时荧光定量PCR和ARMS-PCR进行基因分型。IL-6 -597 GG基因型与pCD风险降低显著相关（p = 0.049）。TNF rs1800629-308 GA基因型有保护作用（p = 0.07）。IL-10 -1082 AA基因型的风险增加趋势无统计学意义（p = 0.07）。TGF-β或mapk相关多态性未发现显著相关性。我们的研究结果表明，细胞因子基因的多态性，特别是IL-6和TNF，可能会影响CD患者发生肛周并发症的风险。这些初步结果需要在更大的队列中进行验证，以评估它们作为pCD个性化管理的预测性生物标志物的潜力。

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引用次数: 0

Clinical value and pro-inflammatory mechanism of miR-503-5p as a novel diagnostic biomarker for Sepsis miR-503-5p作为脓毒症新诊断生物标志物的临床价值及促炎机制

IF 3.7 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Cytokine

Pub Date : 2025-12-01 Epub Date: 2025-11-01 DOI: 10.1016/j.cyto.2025.157060

Yanshan Liu , Tuo Xu , Yeting Zhang , Mali Fang

Aims

Explore the expression of miR-503-5p in sepsis and its influence on the inflammatory response.

Methods

A total of 120 patients with sepsis were retrospectively selected as the research subjects, and 80 healthy individuals were simultaneously selected as the control group. Reverse transcription quantitative polymerase chain reaction was used to detect the expression of miR-503-5p and related genes. Pearson correlation analysis was used to determine the relationships between variables, and multivariate logistic regression analysis was applied to analyze the risk factors associated with sepsis. Receiver operating characteristic curve analysis was performed to assess the diagnostic efficacy of serum miR-503-5p levels for sepsis. Sepsis-model cells were created in vitro for the purpose of exploring the possible action mechanism of miR-503-5p in the context of sepsis.

Results

The expression of miR-503-5p in the serum of patients with sepsis is significantly upregulated, and it is positively correlated with the severity of the patients' condition and inflammatory indexes. miR-503-5p has a good diagnostic potential in sepsis. Furthermore, miR-503-5p exhibited a notable increase in expression within lipopolysaccharide (LPS)-stimulated THP-1 cells, which facilitated the inflammatory process. Mechanistically, miR-503-5p exacerbates the inflammatory response in sepsis via targeted suppression of peroxisome proliferator-activated receptor alpha (PPARA).

Conclusions

During sepsis, miR-503-5p shows elevated expression, and it facilitates the inflammatory response through the targeted suppression of PPARA.

目的探讨miR-503-5p在脓毒症中的表达及其对炎症反应的影响。方法回顾性选取120例脓毒症患者作为研究对象，同时选取80例健康人作为对照组。采用逆转录定量聚合酶链反应检测miR-503-5p及相关基因的表达。采用Pearson相关分析确定变量之间的关系，采用多因素logistic回归分析分析脓毒症相关危险因素。采用受试者工作特征曲线分析，评价血清miR-503-5p水平对脓毒症的诊断效果。体外构建脓毒症模型细胞，探讨miR-503-5p在脓毒症中的可能作用机制。结果脓毒症患者血清中miR-503-5p表达明显上调，且与患者病情严重程度及炎症指标呈正相关。miR-503-5p在脓毒症中具有良好的诊断潜力。此外，miR-503-5p在脂多糖（LPS）刺激的THP-1细胞中表达显著增加，从而促进了炎症过程。在机制上，miR-503-5p通过靶向抑制过氧化物酶体增殖物激活受体α (PPARA)加剧了脓毒症的炎症反应。结论脓毒症时miR-503-5p表达升高，通过靶向抑制PPARA促进炎症反应。

{"title":"Clinical value and pro-inflammatory mechanism of miR-503-5p as a novel diagnostic biomarker for Sepsis","authors":"Yanshan Liu , Tuo Xu , Yeting Zhang , Mali Fang","doi":"10.1016/j.cyto.2025.157060","DOIUrl":"10.1016/j.cyto.2025.157060","url":null,"abstract":"<div><h3>Aims</h3><div>Explore the expression of miR-503-5p in sepsis and its influence on the inflammatory response.</div></div><div><h3>Methods</h3><div>A total of 120 patients with sepsis were retrospectively selected as the research subjects, and 80 healthy individuals were simultaneously selected as the control group. Reverse transcription quantitative polymerase chain reaction was used to detect the expression of miR-503-5p and related genes. Pearson correlation analysis was used to determine the relationships between variables, and multivariate logistic regression analysis was applied to analyze the risk factors associated with sepsis. Receiver operating characteristic curve analysis was performed to assess the diagnostic efficacy of serum miR-503-5p levels for sepsis. Sepsis-model cells were created in vitro for the purpose of exploring the possible action mechanism of miR-503-5p in the context of sepsis.</div></div><div><h3>Results</h3><div>The expression of miR-503-5p in the serum of patients with sepsis is significantly upregulated, and it is positively correlated with the severity of the patients' condition and inflammatory indexes. miR-503-5p has a good diagnostic potential in sepsis. Furthermore, miR-503-5p exhibited a notable increase in expression within lipopolysaccharide (LPS)-stimulated THP-1 cells, which facilitated the inflammatory process. Mechanistically, miR-503-5p exacerbates the inflammatory response in sepsis via targeted suppression of peroxisome proliferator-activated receptor alpha (PPARA).</div></div><div><h3>Conclusions</h3><div>During sepsis, miR-503-5p shows elevated expression, and it facilitates the inflammatory response through the targeted suppression of PPARA.</div></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"196 ","pages":"Article 157060"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145413024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term storage stability of plasma TNF-α and IL-6 concentrations of psychiatric emergency patients: The Signature Biobank 精神急症患者血浆TNF-α和IL-6浓度的长期储存稳定性：特征生物库。

IF 3.7 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Cytokine

Pub Date : 2025-12-01 Epub Date: 2025-10-07 DOI: 10.1016/j.cyto.2025.157033

Enzo Cipriani , Charles-Édouard Giguère , Cécile Le Page , Helen Findlay , Janick Boissoneault , Stéphane Potvin , Robert-Paul Juster

Cytokines are increasingly incorporated in psychiatric research. While biobanking provides advantages for different study designs, long-term storage could degrade cytokine quality and introduce bias. To date, reports on cytokine stability are based on small sample sizes (n < 20) and do not address long-term (4+ years) storage effects. This article reports two studies evaluating IL-6 and TNF-α measurements in human plasma samples biobanked for long periods. In the first study, with samples stored between 5 and 139 months (11.6 years), IL-6 concentrations were not significantly correlated with storage length in all available baseline data of the Signature Biobank (n[IL-6] = 1206, n[TNF-α] = 1223). TNF-α correlated negatively with storage length (r = −0.217; p < 0.001) indicating a decrease of concentrations with longer storage. In the second study, IL-6 and TNF-α concentrations were measured twice in the same plasma samples of 50 psychiatric participants from the Signature Biobank stored between analyses from 32 to 45 months. We assessed if storage effects differed between analytes. In IL-6, we observe a relatively good stability in samples stored up to 6 years. For TNF-α, we observed only a moderate stability of measurements (rTNF-α = 0.59; rIL-6 = 0.71) with linear decrease over time. As a potential solution, a corrective equation extracted from Study 1 was applied to TNF-α in the Study 2 sample; however, this did not improve correlation coefficients but might be useful in other settings. Integrating samples' age in statistical analyses and/or more systematic quality controls could mitigate degradation process.

细胞因子越来越多地被纳入精神病学研究。虽然生物银行为不同的研究设计提供了优势，但长期储存可能会降低细胞因子的质量并引入偏差。迄今为止，关于细胞因子稳定性的报告都是基于小样本量(n

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引用次数: 0