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Rosmarinus officinalis Might be Exploited as a Natural Antifouling Agent: A Potentially Promising Strategy for Curbing Membrane Biofouling 迷迭香可能是一种天然防污剂:抑制膜生物污染的一种潜在的有前途的策略
Pub Date : 2022-03-10 DOI: 10.56946/jspae.v1i1.15
A. Salisu, S. Umar, A. H. Kawo, Cosa Sekelwa
Membrane biofouling is the coverage of membrane surfaces due to undesirable development of biofilms causing a decrease and subsequent loss of productivity in water treatment settings. Continuous use of synthetic chemicals against biofouling is inept as it leads to the emergence of multi-antibiotic resistance. Application of natural products such as plants can be apt in curbing biofouling while checking the resistance challenge. This study aimed to evaluate the potential of Rosmarinus officinalis in the control of membrane biofouling. Bacteria from biofouling environments were subjected to a biofilm confirmation test and identified at cultural, morphological, biochemical and molecular levels. Leaves of R.officinalis were extracted in solvents of varying polarity and activities. These extracts were evaluated against bacterial biofilm formation via minimum biofilm inhibitory concentration (MBIC), minimum biofilm eradication concentration (MBEC) and mesocosm bioassays. Biofilm formation was confirmed in 68% of the isolates identified as Pseudomonas aeruginosa, Klebsiella pneumoniae and Staphylococcus aureus. The methanol and ethyl acetate extracts of R.officinalis indicated the least MICs (0.313mg/L and 1.25mg/L) against Pseudomonas aeruginosa and Staphylococcus aureus, respectively. Both extracts recorded the highest MBIC (50.00%) against Pseudomonas aeruginosa. The peak MBEC (57.88%) was obtained from the methanol extract against Staphylococcus aureus and this same extract inhibited 56.23% density of bacterial biofilms on glass slides.The methanol and ethyl acetate crude extracts of R. officinalis appreciably reduced bacterial biofilms; hence, this plant can be exploited as a natural antifouling agent, with reduced toxicity and low risk of resistance.
膜生物污染是指在水处理环境中,由于生物膜的不良发育导致生产力下降和随后的损失而导致膜表面的覆盖。持续使用合成化学品来对抗生物污垢是不恰当的,因为它会导致多种抗生素耐药性的出现。在检查抗性挑战的同时,应用植物等天然产物可以有效地抑制生物污染。本研究旨在评价迷迭香在防治膜生物污染中的应用潜力。对来自生物污染环境的细菌进行了生物膜确认试验,并在培养、形态、生化和分子水平上进行了鉴定。在不同极性和活性的溶剂中提取山茱萸叶。通过最小生物膜抑制浓度(MBIC)、最小生物膜根除浓度(MBEC)和中观生物测定来评价这些提取物对细菌生物膜形成的影响。经鉴定为铜绿假单胞菌、肺炎克雷伯菌和金黄色葡萄球菌的68%的分离株确认形成生物膜。甲醇和乙酸乙酯提取物对铜绿假单胞菌和金黄色葡萄球菌的mic最低,分别为0.313mg/L和1.25mg/L。两种提取物对铜绿假单胞菌的MBIC均最高(50.00%)。甲醇提取物对金黄色葡萄球菌的MBEC峰值为57.88%,对载玻片上细菌生物膜的抑制密度为56.23%。马蹄草甲醇粗提物和乙酸乙酯粗提物对细菌生物膜有明显的还原作用;因此,这种植物可以作为一种天然的防污剂,具有低毒性和低抗性风险。
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引用次数: 0
Impact of Nitrogen, Zinc and Humic Acid Application on Wheat Growth, Morphological Traits, Yield and Yield Components 施氮、锌和腐植酸对小麦生长、形态性状、产量及产量构成的影响
Pub Date : 2022-02-05 DOI: 10.56946/jspae.v1i1.11
A. Iqbal, H. Raza, M. Zaman, Rayyan Khan, Muhammad Adnan, Abdullah Khan, Syeda Wajeeha Gillani, Shad Khan Khalil
To investigate the response of wheat to different levels of nitrogen (N), zinc (Zn) and humic acid (HA), an experiment was conducted at Agronomy Research Farm, the University of Agriculture, Peshawar, during 2014-15. The experiment was laid out in a randomized complete block design having three replications. Three levels of N (80, 120 and 160 kg ha-1), Zn (6, 12 and18 kg ha-1) and HA (5, 10 and 15 kg ha-1) were used. Results showed that N application at the rate of 160 kg ha-1 manifested maximum days to physiological maturity (164 days), productive tillers m-2 (248), spikes m-2 (258), leaf area tiller-1 (113.6 cm2), spike length (10.4 cm), grains spike-1 (52), 1000-grain weight (47.5 g), biological yield (9260 kg ha-1), grain yield (3723 kg ha-1) and harvest index (40%). Zn treated plots at the rate of 12 kg ha-1 showed maximum days to physiological maturity (162 days), productive tillers m-2 (241), spikes m-2 (252), grains spike-1 (51), 1000-grain weight (45.2 g), biological yield (8843 kg ha-1), grain yield (3375 kg ha-1) and harvest index (39 %). Similarly, HA treated plots at the rate of 12 kg ha-1 revealed maximum days to physiological maturity (162 days), productive tillers m-2 (238), spikes m-2(249), spike length (9.7 cm), 1000-grain weight (45.00 g), biological yield (8649 kg ha-1), grain yield (3342 kg ha-1) and harvest index (39%). The combined application of N, Zn, and HA had significantly affected wheat yield and yield components. It was concluded that N at the rate of 160 kg ha-1, Zn 12 kg ha-1 and HA 10 kg ha-1 significantly increased yield and yield components of wheat.
为了研究小麦对不同氮、锌和腐植酸水平的响应,2014- 2015年在白沙瓦农业大学农学研究农场进行了试验。实验采用随机完全区组设计,有3个重复。施用氮(80、120和160 kg HA -1)、锌(6、12和18 kg HA -1)和HA(5、10和15 kg HA -1) 3个水平。结果表明,施氮量为160 kg hm -1时,植株生理成熟期最长(164 d),分蘖数(248),穗数(258),叶面积(113.6 cm2),穗长(10.4 cm),穗数(52),千粒重(47.5 g),生物产量(9260 kg hm -1),籽粒产量(3723 kg hm -1),收获指数(40%)。12 kg hm -1 Zn处理地块生理成熟期最长(162天),分蘖数最多(241),穗数最多(252),穗数最多(51),千粒重最多(45.2 g),生物产量最多(8843 kg hm -1),籽粒产量最多(3375 kg hm -1),收获指数最高(39%)。同样,施用12 kg HA -1的地块生理成熟最长天数(162天),分蘖数(238),穗数(249),穗长(9.7 cm),千粒重(45.00 g),生物产量(8649 kg HA -1),籽粒产量(3342 kg HA -1)和收获指数(39%)。氮、锌和腐殖酸配施对小麦产量和产量组成有显著影响。综上所述,施氮量为160 kg HA -1、施锌量为12 kg HA -1、施磷肥量为10 kg HA -1均能显著提高小麦产量和产量组成成分。
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引用次数: 2
Impact of Phosphorous and Zinc Levels on the Productivity of Green Gram (Vigna radiate L.) 磷、锌水平对绿克草(Vigna辐射L.)产量的影响
Pub Date : 2022-01-23 DOI: 10.21203/rs.3.rs-1288485/v1
A. Khan
Mung bean is one of the important Kharif pulses in Pakistan and is grown mainly for its edible seeds; therefore, fertilizers management is an important factor for improving mungbean growth and yield. A field experiment was conducted during the summer of 2013 at Palato Farm of the University of Agriculture Peshawar, Amir Muhammad Khan Campus Mardan, to determine the effect of phosphorus (P) and Zinc (Zn) on the yield and yield component of mungbean. The experiment consisted of four levels of P (0, 25, 50, and 75 kg ha−1) and four levels of Zn (0, 5, 10, and 15 kg ha−1). Data associated with the number of leaves and plant height illustrated that the higher number of leaves plant−1 (8.8) by an average was observed when P was applied at the rate of 75 kg ha−1 followed by 0 kg phosphorous (P) ha−1 (8.7) and Zn (Zn) application at the rate of 10 kg ha−1produced a maximum number of leaves plant−1 (9) followed by 15 kg ha−1(8.8) where 0 kg ZN ha−1 resulted in (7.7). Similarly, Zn significantly affected plant height, while P and interaction between P and Zn levels were non-significant. The higher plant height (95.1 cm) was observed when P was applied at the rate of 75 kg ha−1, followed by 50 kg P ha−1 (93.6 cm). Higher plant height (95.8cm) was recorded when ZN was applied at the 5 kg ha−1 followed by 10 kg ha−1(95.1cm). Higher numbers of nodules (13.1) were observed with the application of 50 kg P ha−1 followed by 75 kg P ha−1 (12.3), while the lowest (10.6) nodules were observed in the control plot. P application at the rate of 25 kg ha−1 produced a higher grain yield than 75 and 50 kg ha-1 and Zn application at the rate of 5 kg ha-1 produced a higher grain yield than 10 and 15 kg ha−1. Therefore, a lower rate of P 25 kg ha−1 and Zn 5 kg ha−1 is recommended for a higher yield of mungbean in the agro-ecological condition of Mardan.
绿豆是巴基斯坦重要的Kharif豆类之一,主要因其可食用的种子而种植;因此,肥料管理是提高绿豆生长和产量的重要因素。2013年夏季,在白沙瓦农业大学阿米尔·穆罕默德·汗校区马丹的Palato农场进行了田间试验,以确定磷(P)和锌(Zn)对绿豆产量和产量成分的影响。试验包括4个水平的P(0、25、50和75 kg ha−1)和4个水平的Zn(0、5、10和15 kg ha−1)。与叶片数和株高相关的数据表明,当P以75 kg ha - 1的速率施用时,植株叶片数平均较高(8.8),然后是0 kg磷(P) ha - 1(8.7), Zn (Zn)以10 kg ha - 1的速率施用时,植株叶片数最多(9),然后是15 kg ha - 1(8.8),其中0 kg Zn ha - 1导致(7.7)。同样,Zn对株高有显著影响,而P和P与Zn的交互作用不显著。施磷量为75 kg ha - 1时,株高最高(95.1 cm),施磷量为50 kg ha - 1 (93.6 cm)。5 kg ha - 1施锌,10 kg ha - 1施锌(95.1cm),株高较高(95.8cm)。施用50 kg磷- 1后再施用75 kg磷- 1(12.3),观察到较多的根瘤(13.1),而在对照区观察到最少的根瘤(10.6)。施磷量为25 kg ha-1的籽粒产量高于75和50 kg ha-1,施锌量为5 kg ha-1的籽粒产量高于10和15 kg ha-1。因此,在马尔丹农业生态条件下,建议施用25 kg ha - 1磷肥和5 kg ha - 1锌以获得较高的绿豆产量。
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引用次数: 0
Unprecedented response of wheat to irrigation levels and various rates of Nano-black carbon 小麦对灌溉水平和不同纳米黑碳含量的空前反应
Pub Date : 2021-12-23 DOI: 10.56946/jspae.v1i1.3
Imran
In Khyber-Pakhtunkhwa, Pakistan, wheat yield is subjected to availability of water and proper rate of Nano-black carbon in soil. Delay in rain and unsuitable soil health cause severe yield reduction. Therefore this experiment was conducted to compare Irrigation levels in relation to different rate of Nano-black carbon to find out high yielding fact that could enhance wheat productivity and food security. Three different-irrigation-levels (250-mm, 275-mm and 300-mm), were compared in early growth establishment in three repeats with five different rates of Nano-black carbon (5Mg ha-1, 10Mg ha-1, 15Mg ha-1, 20Mg ha-1 and 25Mg ha-1). All the other agronomic practices were kept similar for each replicate. Data was recoded on different growth parameters such as days to emergence, emergence m-2, plant height, spike length, number of spikes m-2, thousand grain weight and grain yield. The study confirmed that almost all Irrigation levels were prominent but significant reduction in different parameters was observed with variation in Nano-black carbon application that could ultimately effect soil health and productivity. From this experiment we concluded that proper rate of Nano-black carbon can significantly enhance the development of roots system which may ultimately increase the shoot growth and final yield. The present study revealed that wheat Irrigation levels (250 mm) can properly save water and increase wheat productivity in combination with Nano-black carbon addition. Different Irrigation levels retorted differently to different Nano-black carbon showing that potential did exist in Nano-black carbon for water storage and improvement of soil health under drought stress condition.
在巴基斯坦的开伯尔-普赫图赫瓦省,小麦产量取决于土壤中水分的供应和纳米黑碳的适当含量。延迟降雨和不适宜的土壤健康造成严重的减产。因此,本试验旨在通过比较不同纳米黑碳用量与灌溉水平的关系,找出提高小麦产量和粮食安全的高产因素。采用5Mg ha-1、10Mg ha-1、15Mg ha-1、20Mg ha-1和25Mg ha-1 5种不同浓度(250 mm、275 mm和300 mm)的纳米黑碳,在3个重复的生长初期进行了比较。所有其他农艺做法在每个重复中保持相似。对出苗期、出苗期m-2、株高、穗长、穗数m-2、千粒重、籽粒产量等不同生长参数进行数据编码。该研究证实,几乎所有的灌溉水平都是显著的,但观察到不同参数的显著减少,纳米黑碳施用的变化可能最终影响土壤健康和生产力。本试验结果表明,适当的纳米黑碳用量可以显著促进根系发育,最终提高植株的生长和产量。研究表明,小麦灌溉水平(250 mm)与纳米黑碳配合施用可有效节约水分,提高小麦产量。不同灌溉水平对不同纳米黑碳的还原效果不同,表明纳米黑碳在干旱胁迫条件下具有储水和改善土壤健康的潜力。
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引用次数: 9
Impact of late sowing on morphological and yield traits in 40s bread wheat 晚播对40代面包小麦形态和产量性状的影响
Pub Date : 2021-12-01 DOI: 10.56946/jspae.v1i1.2
Muhammad Adnan, Abdullah Khan, F. Mohammad, Fawad Ali, Quaid Hussain
The unpredictability and large fluctuation of the climatic conditions in rainfed regions influences spring wheat yield and grain quality. These variations offer the opportunity for the production of better quality wheat. The effect of late sowing on wheat morphology and grain yield was studied in different 40s bread wheat at the research farm of PBG, The University of Agriculture Peshawar, Pakistan during 2013-14. Forty wheat genotypes were tested under normal and late sowing in 5 × 8 alpha lattice design with three replicates. Combined analysis of variance exhibited significant genotype by environment interactions for days to heading, flag leaf area, days to maturity, plant height, spikes m-2, grains spike-1,1000-grain weight, biomass yield, grain yield and harvest index.  Days to emergence, headings, maturity ranged from 9 to 12, 111 to 121 and 155 to 164 days under normal while under late planting it ranged from 25 to 29, 95 to107 and 137 to 143 days. Mean data under normal planting ranged between 77 to 125cm; 25 to 41cm2; 99 to 199; 10 to 13 cm 32 to 49; 52 to 88g; 8533 to 13667 kg, 1869 to 4681 kg; 21 to 35% whereas under late planting its range was 63 to 91 cm, 18 to 37 cm2, 57 to 137, 8 to 12 cm, 22 to 52, 36 to 75g, 2400 to 7933 kg, 540 to 2739 kg and 20 to 42% for plant height, flag leaf area, spikes m-2, spike length, grains spike-1, 1000-grain weight, biomass, grain yield and harvest index, respectively. Wheat genotypes planted at late condition took maximum days to emergence, while less number of days were reacquired for wheat genotypes planted at normal sowing date to get mature. Late planting negatively affected all yield contributing traits like; spikes m-2 (29%), grains spike-1 (18%) 1000-grain weight (29 %), biomass (55%) and grain yield (50 %). On the basis of the current exploration, it is obtained that genotype SRN 19111 was identified superior for 1000-grain weight, biomass yield and grain yield under normal planting, while genotype PR-107 exhibited higher grain yield under late planting. Therefore, these genotypes are recommended for further extensive testing.
旱旱区气候条件的不可预测性和波动较大,影响着春小麦产量和籽粒品质。这些变化为生产质量更好的小麦提供了机会。2013- 2014年,在巴基斯坦白沙瓦农业大学PBG研究农场,研究了晚播对不同40代面包小麦形态和产量的影响。采用5 × 8 α晶格设计,3个重复,对40个小麦基因型进行了正常和晚播试验。综合方差分析显示,抽穗期、旗叶面积、成熟期、株高、穗m-2、穗1、千粒重、生物量产量、籽粒产量和收获指数的环境互作均具有显著的基因型。出苗期、抽穗期、成熟期分别为9 ~ 12天、111 ~ 121天和155 ~ 164天,晚播期分别为25 ~ 29天、95 ~ 107天和137 ~ 143天。正常种植下的平均数据在77至125厘米之间;25 ~ 41cm2;99 ~ 199;10至13厘米32至49;52至88克;8533 ~ 13667公斤,1869 ~ 4681公斤;株高、旗叶面积、穗m-2、穗长、穗1、穗重、千粒重、生物量、籽粒产量和收获指数分别为63 ~ 91 cm、18 ~ 37 cm2、57 ~ 137、8 ~ 12 cm、22 ~ 52、36 ~ 75g、2400 ~ 7933 kg、540 ~ 2739 kg和20 ~ 42%。晚播小麦基因型的出苗期最长,而正常播期小麦基因型的成熟期较短。晚播对产量贡献性状均有负向影响,如;穗m-2(29%)、穗1(18%)、千粒重(29%)、生物量(55%)和籽粒产量(50%)。结果表明,SRN 19111基因型在正常种植条件下具有较好的千粒重、生物量产量和籽粒产量,PR-107基因型在晚播条件下具有较高的籽粒产量。因此,建议对这些基因型进行进一步的广泛检测。
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引用次数: 2
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Journal of Soil, Plant and Environment
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