Cells & Development最新文献

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ZebRack: A budget-friendly mobile system for short-term zebrafish housing. ZebRack：一个预算友好的移动系统，用于短期的斑马鱼住房。

IF 2 4区生物学 Q3 DEVELOPMENTAL BIOLOGY

Cells & Development

Pub Date : 2026-03-05 DOI: 10.1016/j.cdev.2026.204074

Pauline Sallaberry-Pincheira, Aarón Villanueva, Leonardo E Valdivia

The zebrafish (Danio rerio) has become a popular model organism in developmental biology, but the high costs of commercial housing systems often limit accessibility for resource-constrained research and educational purposes. Here, we introduce ZebRack, an open-source, budget-friendly mobile zebrafish housing system designed to support autonomous maintenance and breeding during short term periods. Built for under US$900, this modular 15-tank setup features 2.8-l tanks integrated into a compact, mobile framework with recirculating water filtration and adjustable flow rates. The system prioritizes cost-efficiency through off-the-shelf hardware and components while ensuring water quality via a three-stage filtration process (mechanical, biological, and chemical). Temperature is precisely controlled using a submersible water heater and can be complemented with a portable air conditioning unit to maintain optimal conditions. ZebRack demonstrated robust performance in a proof-of-principle trial during the 2025 International Developmental Biology Course in Quintay, Chile. Operated within an indoor culture tent, the system enabled daily embryo production via an integrated electric photoperiod system to synchronize breeding cycles. The modular design of ZebRack allows for quick setup and configuration, accommodating diverse experimental or teaching needs, while its portability and small footprint make it ideal for laboratories with limited space or temporary setups. By combining affordability with open-access design specifications, validated thermal stability, and field-tested breeding efficiency, this system adds flexibility to current zebrafish husbandry infrastructure. Detailed assembly guides and part lists are freely available to promote global adoption, fostering equitable access to zebrafish-based research and education.

斑马鱼（Danio rerio）已成为发育生物学中流行的模式生物，但商业住房系统的高成本往往限制了资源有限的研究和教育目的的可及性。在这里，我们介绍了ZebRack，一个开源的、预算友好的移动斑马鱼住房系统，旨在支持短期内的自主维护和繁殖。这款模块化的15缸装置售价不到900美元，其2.8升的水箱集成在一个紧凑的移动框架中，具有循环水过滤和可调节的流量。该系统通过现成的硬件和组件优先考虑成本效益，同时通过三级过滤过程（机械、生物和化学）确保水质。使用潜水式热水器精确控制温度，并可与便携式空调装置相辅相成，以保持最佳状态。在智利昆泰举行的2025年国际发育生物学课程上，ZebRack在一项原理验证试验中表现出了强劲的性能。该系统在室内培养帐篷内运行，通过集成的光电周期系统实现每日胚胎生产，以同步育种周期。ZebRack的模块化设计允许快速设置和配置，适应不同的实验或教学需求，而其便携性和占地面积小，使其成为空间有限或临时设置的实验室的理想选择。通过将可负担性与开放式设计规范、经过验证的热稳定性和现场测试的繁殖效率相结合，该系统为当前的斑马鱼养殖基础设施增加了灵活性。详细的装配指南和零件清单免费提供，以促进全球采用，促进公平获得基于斑马鱼的研究和教育。

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引用次数: 0

Inflammation and oxidative stress impair preimplantation embryonic morphogenesis in allergic asthma model. 炎症和氧化应激对过敏性哮喘模型着床前胚胎形态发生的影响。

IF 3.9 4区生物学 Q3 DEVELOPMENTAL BIOLOGY

Cells & Development

Pub Date : 2023-06-13 DOI: 10.2139/ssrn.4367239

Che Ismail Wafriy, Y. S. Kamsani, M. Nor-Ashikin

The incidence of allergic asthma has been increasing worldwide in recent decades. Also, an increasing number of women are suffering from poor pregnancy outcome. However, the causal relationship between allergic asthma and embryonic growth in terms of cell morphogenesis has not been well elucidated. Here, we investigated the impact of allergic asthma on the morphogenesis of preimplantation embryos. Twenty-four female BALB/c were randomly divided into control (PBS), 50-μg (OVA1), 100-μg (OVA2) and 150-μg (OVA3). On Days-0 and -14, mice were induced intraperitoneally (i.p) with ovalbumin (OVA). On Days-21 until -23, mice were challenged with OVA via intranasal instillation (i.n). Control animals were sensitized and challenged with PBS. At the end of treatment (Day-25), 2-cell embryos were retrieved and cultured in vitro until the blastocysts hatched. Results showed reduced number of preimplantation embryos at all developing stages in all treated groups (p ≤ 0.0001). Uneven blastomere size, partial compaction- and cavitation-activity, low formation of trophectoderm (TE), as well as cell fragmentation were noted in all the treated groups. Maternal serum interleukin (IL)-4, immunoglobulin (Ig)-E and 8-hydroxydeoxyguanosine (8-OHdG) were notably high (p ≤ 0.0001, p ≤ 0.01) in contrast with low total antioxidant capacity (TAOC) (p ≤ 0.0001). Our findings indicated that OVA-induced allergic asthma had compromised cell morphogenesis through reduced blastomere cleavage division, partial compaction and cavitation-activity, impairment of TE production, and cell fragmentation leading to embryonic cell death via OS mechanism.

近几十年来，过敏性哮喘的发病率在全球范围内呈上升趋势。此外，越来越多的妇女遭受怀孕结果不佳的痛苦。然而，过敏性哮喘与胚胎发育之间在细胞形态发生方面的因果关系尚未得到很好的阐明。本研究探讨过敏性哮喘对着床前胚胎形态发生的影响。24只雌性BALB/c随机分为对照组(PBS)、50 μg (OVA1)、100 μg (OVA2)和150 μg (OVA3)。在第0天和第14天，小鼠腹腔注射卵清蛋白(OVA)。在第21天至第23天，小鼠通过鼻内滴注(i.n)注射OVA。对照动物用PBS致敏和刺激。在处理结束时(第25天)，取出2细胞胚胎并在体外培养，直到囊胚孵化。结果显示，所有处理组在所有发育阶段的着床前胚胎数量均减少(p ≤ 0.0001)。在所有处理组中，卵裂球大小不均匀，部分压实和空化活性，滋养外胚层(TE)形成低以及细胞碎裂都被注意到。血清白细胞介素(IL)-4、免疫球蛋白(Ig)-E和8-羟基脱氧鸟苷(8-OHdG)含量显著高(p ≤ 0.0001,p ≤ 0.01)，而总抗氧化能力(TAOC)较低(p ≤ 0.0001)。我们的研究结果表明，ova诱导的过敏性哮喘通过降低卵裂球切割分裂、部分压实和空化活性、TE产生障碍以及通过OS机制导致胚胎细胞死亡的细胞分裂来破坏细胞形态发生。

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引用次数: 1

Disrupted neurogenesis, gliogenesis, and ependymogenesis in the Ccdc85c knockout rat for hydrocephalus model. 脑积水模型中Ccdc85c基因敲除大鼠神经发生、胶质瘤发生和室管膜形成被破坏。

IF 3.9 4区生物学 Q3 DEVELOPMENTAL BIOLOGY

Cells & Development

Pub Date : 2023-06-01 DOI: 10.2139/ssrn.4367238

Mehedi Hasan, Shizuka Konishi, Miyuu Tanaka, T. Izawa, J. Yamate, M. Kuwamura

Coil-coiled domain containing 85c (Ccdc85c) is a causative gene for congenital hydrocephalus and subcortical heterotopia with frequent brain hemorrhage. We established Ccdc85c knockout (KO) rats and investigated the roles of CCDC85C and intermediate filament protein expression, including nestin, vimentin, GFAP, and cytokeratin AE1/AE3 during the lateral ventricle development in KO rats to evaluate the role of this gene. We found altered and ectopic expression of nestin and vimentin positive cells in the wall of the dorso-lateral ventricle in the KO rats during development from the age of postnatal day (P) 6, whereas both protein expression became faint in the wild-type rats. In the KO rats, there was a loss of cytokeratin expression on the surface of the dorso-lateral ventricle with ectopic expression and maldevelopment of ependymal cells. Our data also revealed disturbed GFAP expression at postnatal ages. These findings indicate that lack of CCDC85C disrupts the proper expression of intermediate filament proteins (nestin, vimentin, GFAP, and cytokeratin), and CCDC85C is necessary for normal neurogenesis, gliogenesis, and ependymogenesis.

含有85c的螺旋结构域(Ccdc85c)是先天性脑积水和皮质下异位并频繁脑出血的致病基因。我们建立了Ccdc85c敲除(KO)大鼠，研究了Ccdc85c和中间丝蛋白表达，包括巢蛋白、波形蛋白、GFAP和细胞角蛋白AE1/AE3在KO大鼠侧脑室发育中的作用，以评估该基因的作用。我们发现，从出生后6天开始，KO大鼠的背侧脑室壁中巢蛋白和波形蛋白阳性细胞的表达发生了改变和异位，而野生型大鼠的这两种蛋白的表达都变得微弱。在KO大鼠中，背侧脑室表面细胞角蛋白表达缺失，室管膜细胞异位表达和发育不良。我们的数据还显示出生后GFAP表达紊乱。这些发现表明，缺乏CCDC85C会破坏中间丝蛋白(巢蛋白、波形蛋白、GFAP和细胞角蛋白)的正常表达，而CCDC85C对于正常的神经发生、胶质形成和室管膜形成是必需的。

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引用次数: 1

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