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Presença do ossículo meniscal na articulação femorotibial de gatos-domésticos (Felis catus, Linnaeus, 1758) 家猫股骨颈关节半月板骨化的存在(猫,林奈,1758)
Pub Date : 2020-05-25 DOI: 10.5007/2175-7925.2020.e71297
Wiliam da Silva Prieto, A. Carvalho, L. C. S. Silva
A articulação femorotibial é uma articulação complexa do tipo sinovial, que, além da relação com a patela, em algumas espécies de animais pode apresentar uma estrutura radiopaca visualizada ao exame radiográfico de formato oval entre os ossos do fêmur e tíbia, em projeção mediolateral, denominado ossículo meniscal. Embora comumente relatado em diferentes espécies, inclusive no homem, pouco se sabe sobre a sua função e frequência, sendo bem constatada sua existência em espécimes de grandes felídeos. O presente estudo teve como objetivo realizar a análise radiográfica da articulação femorotibiopatelar de 40 gatos-domésticos (Felis catus, Linnaeus, 1758) em projeções mediolateral e craniocaudal no intuito de avaliar a presença ou ausência do ossículo meniscal. Nas radiografias realizadas, o ossículo esteve presente em 15% (n = 6), observando-se sua maior frequência em machos e unilateralmente, sendo o joelho esquerdo o de maior incidência da estrutura. Por ser um achado comum e relatado em diversas espécies selvagens de felídeos, a presença do ossículo meniscal deve ser levada em consideração e inserida como diagnóstico diferencial quando da realização de radiografias de gatos-domésticos e da visualização de opacidade intra-articular em projeções radiográficas mediolaterais da articulação femorotibiopatelar.
股骨-胫骨关节是一种复杂的滑膜关节,除了与髌骨的关系外,在一些动物物种中,股骨和胫骨之间的椭圆形放射学检查中可能会显示出不透射线的结构,称为半月板小骨。尽管在包括人类在内的不同物种中都有报道,但人们对其功能和频率知之甚少,而且在大型猫科动物标本中也观察到了它的存在。本研究旨在对40只家猫(Felis catus,Linnaeus,1758。在进行的X线片中,骨化出现率为15%(n=6),在男性和单侧观察到其最高频率,左膝是该结构发生率最高的一个。因为这是一种常见的发现,并且在几种野生猫科动物中都有报道,所以在对家猫进行放射学检查和在股骨颈关节的内侧放射学投影中观察关节内混浊时,应该考虑半月板骨化的存在,并将其作为鉴别诊断。
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引用次数: 0
Avaliação de frutos de goiabeira-serrana cultivados no meio oeste de Santa Catarina, Brasil 巴西圣卡塔琳娜中西部种植番石榴果实的评价
Pub Date : 2020-05-25 DOI: 10.5007/2175-7925.2020.e70318
Fernando David Sánchez-Mora, L. Saifert, V. Petry, L. J. Borsuk, Juan Manuel Otalora Villamil, R. Nodari
The aim of the present study was to determine the behavior of physicochemical characteristics of feijoa fruits of seven genotypes grown in two locations of Santa Catarina. The evaluation was made using the following: (i) 2011/12, 2014/15 and 2015/16 harvests of genotypes 85, 101, 223, 458 and 509, cultivated in Fraiburgo (Papua community); and (ii) 2014/15 and 2015/16 harvests of genotypes 28.12 and 527, in Agua Doce (Vista Alegre community). Diameter (cm), length (cm) and weight (g) of the fruit, peel (g) and pulp (g) weight, peel thickness (mm), and total soluble solids (Brix grade) were evaluated. An analysis of variance, comparisons between means using Tukey’s test (p < 0.05), and Pearson correlation coefficients (p < 0.05) were computed for the variables of feijoa fruits. In Agua Doce, genotype 527 had the largest diameter and weight of the fruit (88.7 g). In Fraiburgo, genotype 85 had the highest fruit weight (94.9 g) and genotype 509 had the highest pulp yield (37.5%). Not all genotypes with higher fruit weight exhibited higher pulp yield, confirming that the skin thickness and fruit size variables are directly related to yield. The variation in total soluble solids values was due to the environment and genotype effects.
本研究的目的是测定生长在圣卡塔琳娜州两个地区的七个基因型的菲荷阿果实的理化特性。评估使用以下内容:(i)2011/12、2014/15和2015/16年在Fraiburgo(巴布亚社区)种植的基因型85、101、223、458和509的收成;以及(ii)在Agua Doce(Vista Alegre群落)的基因型28.12和527的2014/15和2015/16收获。对果实的直径(cm)、长度(cm)和重量(g)、果皮(g)和果肉(g)重量、果皮厚度(mm)和总可溶性固形物(Brix等级)进行评估。采用Tukey检验(p<0.05)和Pearson相关系数(p<0.05)计算了肥荷果变量的方差分析、平均值之间的比较。在Agua-Doce中,基因型527的果实直径和重量最大(88.7g)。在Fraiburgo中,基因型85的果实重量最高(94.9g),基因型509的果肉产量最高(37.5%)。并非所有果实重量较高的基因型都表现出较高的果肉产量,这证实了果皮厚度和果实大小变量与产量直接相关。可溶性固形物总量的变化是由于环境和基因型的影响。
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引用次数: 0
In vitro phytotoxicity of Mikania laevigata Schultz Bip. ex Baker extracts on Lactuca sativa L. and Bidens pilosa L. 紫薇的体外植物毒性研究。ex Baker提取物对Lactuca sativa L.和鬼针草的影响。
Pub Date : 2020-05-25 DOI: 10.5007/2175-7925.2020.e68109
Lívia Maria de Lima Santos, A. H. Gonçalves
Plant extracts have secondary metabolites that can act as bioherbicides, with the advantage of being more environmentally friendly. Mikania laevigata belongs to the family Asteraceae and has coumarin 1,2-benzopyrone as a chemical marker, which is capable of inhibiting or stimulating the germination and growth of adjacent plants. Thus, the objective of this work was to evaluate the phytotoxicity of aqueous and ethanolic extracts of M. laevigata on Lactuca sativa (lettuce) and Bidens pilosa (beggarticks). Five concentrations (0.1, 1.0, 1.5, 2.0 and 3.0 mg.mL-1) of aqueous and ethanolic extracts were tested in separate experiments, with distilled water as the control. Germination percentage, germination speed index, initial growth and the content of hydrogen peroxide (H2O2) and malonaldehyde (MDA) were evaluated. In the presence of the ethanolic extract, germination of the species (lettuce and beggarticks) was reduced by 85% and 90%, respectively. Radicle length, for the lowest concentration of the extracts, was not affected. However, at a concentration of 2.0 mg.mL-1 the reductions were 85% for lettuce and 65% for beggarticks. The increase of H2O2 content depended on the dose, where the greater the concentration of the extracts, the greater the production of peroxide, followed by an increase in MDA for lettuce and beggarticks. Seedlings grown in a concentration of 3.0 mg.mL-1 of the ethanolic extract were subjected to necrosis, making subsequent analyses impossible. The M. laeviagata extracts at concentrations of 2.0 and 3.0 mg.mL-1 demonstrated phytotoxicity, with increased oxidative stress in lettuce and beggarticks.
植物提取物具有次生代谢物,可作为生物除草剂,具有更环保的优点。薇甘菊(Mikania laevigata)属于菊科植物,含有香豆素1,2-苯并吡酮作为化学标记物,能够抑制或刺激邻近植物的萌发和生长。因此,本研究的目的是评价水提液和醇提液对莴苣(Lactuca sativa)和乞丐花(Bidens pilosa)的植物毒性。以蒸馏水为对照,分别以0.1、1.0、1.5、2.0、3.0 mg.mL-1 5种浓度的水提液和乙醇提液进行实验。测定发芽率、萌发速度指数、初始生长量、过氧化氢(H2O2)和丙二醛(MDA)含量。在乙醇提取物的作用下,莴苣和乞丐菜的萌发率分别降低了85%和90%。对于最低浓度的提取物,胚根长度不受影响。然而,当浓度为2.0 mg时。mL-1对生菜和乞丐的影响分别为85%和65%。H2O2含量的增加与剂量有关,其中提取物浓度越大,过氧化氢的产生越多,其次是莴苣和乞丐的MDA含量增加。幼苗在3.0毫克的浓度下生长。mL-1的乙醇提取物受到坏死,使后续分析无法进行。提取浓度分别为2.0、3.0 mg。mL-1表现出植物毒性,在莴苣和乞丐中增加氧化应激。
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引用次数: 1
Determinação da metodologia para o teste de germinação de sementes de Eucalyptus urophylla S. T. Blake (Myrtaceae) 尾叶桉种子萌发试验方法的测定(桃金娘科)
Pub Date : 2020-05-25 DOI: 10.5007/2175-7925.2020.e70268
Tatiane Sanches Jeromini, Thaís Pimenta Ferreira da Cruz, Thaís Soares Pereira, G. Silva, Cibele Chalita Martins
Eucalyptus urophylla S. T. Blake e uma das principais especies de eucaliptos cultivadas. Entretanto, nao existem recomendacoes para o teste padrao de germinacao de E. urophylla . Assim, o objetivo foi determinar a melhor metodologia para a conducao do teste de germinacao de sementes de E. urophylla . O experimento foi dividido em duas etapas. Na primeira, foram testados dois substratos (sobre papel mata-borrao e areia) e seis temperaturas (constantes de 15, 20, 25, 30, 35oC e alternada de 20-30oC, entre noite e dia, respectivamente) na conducao do teste de germinacao. Na segunda etapa, foi selecionado o substrato mais indicado no experimento anterior e testado em dois lotes, nas seis temperaturas citadas anteriormente. Foram avaliadas a germinacao, primeira contagem, indice de velocidade e tempo medio de germinacao. O experimento foi realizado em delineamento inteiramente casualizado com quatro repeticoes e os tratamentos foram dispostos em esquema fatorial de 2 x 6 para ambas as etapas. Na comparacao da germinacao entre os lotes, observou-se que o lote 1 apresentou menor desempenho do que o lote 2 nas condicoes de temperaturas extremas. O teste de germinacao de sementes de E. urophylla deve ser realizado a 30 ou 35°C com semeadura sobre papel ou areia, e com contagem inicial e final aos quatro e 10 dias apos a semeadura, respectivamente. Com base nesta pesquisa, mais testes podem ser realizados, para outros lotes e sementes de E. urophylla de outras procedencias, para garantir a confirmacao dos resultados.
尾叶桉是我国主要栽培桉树品种之一。然而,目前还没有关于尾叶E.urophylla标准发芽试验的建议。因此,目的是确定进行尾叶E.urophylla种子发芽试验的最佳方法。实验分为两个阶段。在第一个试验中,在进行发芽试验时,测试了两种基质(在抹纸和沙子上)和六种温度(分别为15、20、25、30、35℃的恒定温度和20-30℃的交替温度)。在第二阶段中,选择前一实验中显示最多的基质,并在上述六个温度下分两批进行测试。对发芽率、初计数、速度指数和平均发芽时间进行了评价。实验以完全随机的设计进行,重复四次,两个阶段的治疗均按2×6的析因方案进行。在批次之间的发芽比较中,观察到在极端温度条件下,批次1表现出比批次2低的性能。尾叶E.urophylla种子的发芽试验应在30或35°C下进行,在纸上或沙子上播种,并分别在播种后4天和10天进行初始和最终计数。在这项研究的基础上,可以对其他批次的尾叶E.urophylla种子进行进一步的测试,以确保结果的确认。
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引用次数: 0
Pescadores e aves marinhas: etnobiologia de uma comunidade pesqueira no sul do Brasil 渔民和海鸟:巴西南部渔业社区的民族生物学
Pub Date : 2020-05-25 DOI: 10.5007/2175-7925.2020.e71987
Mariana Scain Mazzochi, C. J. Carlos
Comunidades dependentes de recursos pesqueiros combinam informações acerca do comportamento da ictiofauna com características oceanográficas e interações ecológicas. Esse conhecimento é definido como tradicional: um complexo entre conhecimento histórico, prático e crenças, os quais evoluem por processos adaptativos e são transmitidos através das gerações. No litoral norte do Rio Grande do Sul, uma comunidade estabelecida nas margens do Rio Tramandaí realiza pesca de emalhe. A interação com animais marinhos ocorre em todas as artes de pesca na região, e a captura incidental de aves marinhas é registrada no sul do país. Predadoras de topo, as aves interagem com barcos pesqueiros em busca de alimento. O objetivo do presente estudo é explorar o conhecimento dos pescadores sobre as aves marinhas. Doze pescadores de Imbé foram entrevistados. Nas narrativas escritas, utilizou-se a análise de discurso. Os pescadores identificam as espécies de aves e as relacionam ao ambiente, reconhecem seus ciclos de vida, e utilizam seus comportamentos para localizar o recurso pesqueiro e obter dados oceanográficos e meteorológicos para aprimorar a navegação. Dessa forma, verificou-se que o conhecimento sobre as aves é uma variável para a orientação no oceano e para a realização de uma pesca efetiva.
依赖渔业资源的社区将有关鱼类种群行为的信息与海洋学特征和生态相互作用结合起来。这种知识被定义为传统知识:历史知识、实践知识和信仰之间的复杂关系,通过适应过程演变并代代相传。在南大北海岸,一个建立在tramandai河岸边的社区进行刺网捕鱼。在该地区的所有渔具中都有与海洋动物的相互作用,在该国南部也有偶然捕获海鸟的记录。作为顶级掠食者,鸟类与渔船互动寻找食物。本研究的目的是探讨渔民对海鸟的知识。12名imbe渔民接受了采访。在书面叙述中,我们使用了话语分析。渔民识别鸟类种类并将它们与环境联系起来,识别它们的生命周期,并利用它们的行为来定位鱼类资源,获取海洋和气象数据,以改善导航。因此,我们发现鸟类知识是海洋定位和有效捕鱼的一个变量。
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引用次数: 3
Population biology and relative growth of the crab Minuca mordax (Smith, 1870) (Crustacea, Decapoda, Ocypodidae) in the Igaraçu River, Parnaíba, state of Piauí, Brazil 巴西Piauí州Parnaíba igara<s:1>河中Minuca mordax (Smith, 1870)(甲壳纲,十足目,鳉科)的种群生物学和相对生长
Pub Date : 2020-05-25 DOI: 10.5007/2175-7925.2020.e67620
Sidely Gil Alves Vieira dos Santos, Luiz Gonzaga Alves dos Santos Filho, L. C. Fernandes-Góes, J. M. Góes
This study aimed to investigate the population biology of Minuca mordax in the Igaracu River, Parnaiba, analyzing frequency distribution in size classes, reproductive period, relative growth, morphological sexual maturity, sex ratio and heterochely. The crabs were manually collected, randomly, by two people for 1 hour, in the representative months of the dry season (September to November) and rainy (April to June). In laboratory the crabs were identified, separated according to sex and measured. A total of 610 samples were captured, with 453 males and 157 females. The average size of carapace width (CW) was significantly different (p < 0.05) between males (17.21 ± 3.68 mm) and females (15.06 ± 3.18 mm). The distribution of the crabs in size classes was unimodal for both sexes. The morphological sexual maturity obtained for males was 12.42 mm CW and females was 10.05 mm CW. The sex ratio for the population was 1:0.35 (male: female), with significant difference from the expected ratio of 1:1. The heterochely was observed only in males of the species (224 right-handed and 229 sinister). Subsequently, other on-site observations were carried out monthly, from February/2011 to January/2012 to observe ovigerous females and determine the reproductive period. Females with eggs were observed only from March to June, corresponding to the rainy season in the region. The results found in the present study are similar to those observed in other populations of violinist crabs and suggest that the population is stable in the environment. The present study provides subsidies for the conservation of the species in the Igaracu River.
本研究旨在研究Parnaiba Igaracu河水貂的种群生物学,分析其在大小级、繁殖期、相对生长、形态性成熟度、性别比和异源性等方面的频率分布。螃蟹是由两个人在旱季(9月至11月)和雨季(4月至6月)的代表性月份随机手动采集的,采集时间为1小时。在实验室里,对螃蟹进行了鉴定、性别分离和测量。共采集610个样本,其中男性453个,女性157个。雄性(17.21±3.68mm)和雌性(15.06±3.18mm)的平均甲宽(CW)有显著差异(p<0.05)。螃蟹在大小类中的分布对两性来说都是单一的。雄性获得的形态性成熟度为12.42毫米CW,雌性为10.05毫米CW。人群的性别比为1:0.35(男:女),与预期的1:1的比例有显著差异。这种异形现象只在该物种的雄性(224只右手,229只阴险)中观察到。随后,从2011年2月至2012年1月,每月进行其他现场观测,以观察产卵雌性并确定繁殖期。只在3月至6月观察到有卵的雌性,这与该地区的雨季相对应。本研究中发现的结果与在其他小提琴螃蟹种群中观察到的结果相似,表明该种群在环境中是稳定的。本研究为Igaracu河物种的保护提供了补贴。
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引用次数: 2
Germinação e desenvolvimento de mudas do tomateiro Super Marmande submetidos à aplicação de giberelina em diferentes níveis de sombreamento 赤霉素在不同遮荫条件下对番茄幼苗萌发发育的影响
Pub Date : 2020-05-25 DOI: 10.5007/2175-7925.2020.e70102
Ramonn Diego Barros de Almeida, C. M. Hüther, A. Langaro, N. Correa, Júlia Conde Oliveira, Daniela Marques Correa, J. Azevedo
Due to the tomato growing socioeconomic importance, issues about the germination and the vegetative growth of the species are the aim of studies focusing on its improvement, seeking mostly to increase productivity. Therefore, this work aimed to evaluate the influence of the gibberellic acid and different levels of shading in the germination and initial development of tomato seedlings from the Super Marmande variety. The experimental outline adopted was in randomized blocks, arranged in factorial scheme (2x3), managing the factor A with and without the application of the hormone, Gibberellin (GA), at a concentration of 200 mg.L-1, and the factor B in three different levels of luminous intensity (full sun (0%), 70 and 90% shading). The germination and the conduction of the experiment were carried out in polyethylene trays with commercial substrate Terral®, composed of 20 plants with 10 repetitions each and the evaluations were carried out 26 days after sowing (DAS). Analysis were led on germination rate, germination speed index, germination percentage, fresh mass, fresh mass, total dry biomass, root volume, stem diameter, root length, the height of aerial part and photosynthetic pigment. Results pointed out that only the levels of shading affected the germination. The application of gibberellic acid for the Super Marmande tomato did not reveal a significant effect. The development of seedlings in the shading of 70% and 90% was better when compared to the full sun treatment.
由于番茄生长的社会经济重要性,有关该物种的发芽和营养生长的问题是研究的重点,主要是为了提高生产力。因此,本研究旨在评价赤霉素酸和不同遮光水平对超级马曼德品种番茄幼苗萌发和初期发育的影响。试验大纲采用随机分组,按因子方案(2x3)排列,在使用或不使用激素赤霉素(Gibberellin, GA)时对因子A进行管理,浓度为200 mg。L-1,以及因子B在三个不同的发光强度水平(全太阳(0%),70%和90%遮光)。在聚乙烯培养皿中进行发芽和传导试验,培养皿采用商用底物Terral®,共20株,每株重复10次,播种后26天进行评价。对发芽率、发芽速度指数、发芽率、鲜质量、鲜质量、总干生物量、根体积、茎粗、根长、地上部高度和光合色素进行分析。结果表明,只有遮荫程度对发芽有影响。赤霉素酸在超级马曼德番茄上的施用效果不显著。70%和90%遮荫条件下的幼苗发育优于全光照条件。
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引用次数: 1
Padrão reprodutivo, dieta e parasitologia de Artibeus lituratus (Olfers, 1818) (Mammalia, Chiroptera) em parques urbanos do município do Rio de Janeiro (Rio de Janeiro, Brasil) 里约热内卢城市公园里的lituratus (Olfers, 1818)(哺乳动物,手翅目)的繁殖模式、饮食和寄生虫学(里约热内卢de Janeiro,巴西)
Pub Date : 2020-05-25 DOI: 10.5007/2175-7925.2020.e70047
Patrícia Gonçalves Guedes, S. Silva, K. D. C. Santos, Alexandre Maurício Carneiro, L.M.D.S. Lopes, Janine Fernandes de Carvalho
This work reports data about the reproductive dynamics, dietary pattern and associations with parasites of Artibeus lituratus specimens captured from 2009 to 2018 in three urban natural parks in the municipality of Rio de Janeiro. The animals were captured with mist nets, measured, weighed and inspected for ectoparasites; whenever possible, feces were collected and blood smears prepared for dietary analysis and presence of hemoparasites, respectively. Based on an evaluation of 307 captured specimens, the biometric data were within the expected for the species. Diet was evaluated from 68 fecal samples, which contained insect fragments, and fruit pulp and seeds of Urticaceae, Combretaceae, Myrtaceae, Piperaceae, and Solanaceae. The parasites found in association with the bats were the Diptera Paratrichobius longicrus and the mite Periglischrus iheringi, in addition to the hemoparasites Plasmodium sp., Hepatozoon sp., and Litomosoides sp. Data about the reproduction of A. lituratus in these urban parks show that this species maintains a reproductive pattern typical of Stenodermatinae, which consists of bimodal polyestry with peaks in March and April and in July, August and September.
这项工作报告了2009年至2018年在巴西里约热内卢市三个城市自然公园捕获的东坡Artibeus lituratus标本的生殖动态、饮食模式及其与寄生虫关系的数据。用雾网捕获动物,测量、称重并检查体外寄生虫;尽可能收集粪便,准备血涂片,分别用于饮食分析和血液寄生虫的存在。根据对捕获的307个标本的评估,该物种的生物特征数据在预期范围内。对68份含昆虫碎片、荨麻科、菊科、桃金桃科、胡椒科和茄科的果肉和种子的粪便样本进行了饲粮评价。在城市公园内发现的寄生虫主要有长颈副翅双翅目寄生虫和长毛螨,以及血寄生虫疟原虫、肝虫疟原虫和毛螨。城市公园内的毛蠓的繁殖数据表明,毛蠓保持窄皮目昆虫的典型繁殖模式,为双峰型,繁殖高峰在3、4月和7、8、9月。
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引用次数: 3
Incidence of the retromolar foramen in dry mandibles and its significance for dental surgical procedures 干燥下颌骨磨牙后孔的发生率及其在牙科手术中的意义
Pub Date : 2020-03-13 DOI: 10.5007/2175-7925.2020.e66995
Gislainy Thais de Lima Lemos, Anderson Alves da Silva Bezerra, S. Alves, Sidiane Barros da Silva, Taciana Rocha dos Santos, C. Magalhães
The retromolar foramen and retromolar canal as well are anatomical variations of the mandible that occur in the region of the retromolar trigone. This foramen and canal contain neurovascular structures that provide additional innervation to the lower molars and buccal area. This study aimed to determine the incidence and occurrence between the sexes of the retromolar foramen in mandibles of the Contemporary Bone Collection of the Federal University of Pernambuco. Sixty-one dry mandibles were analyzed, 37 from males and 24 from females. Of the 61 mandibles, 18 had retromolar foramina (29.5%), and 12 had a unilateral foramen (19.67%). Male mandibles had a higher incidence compared to female bones, with 12 (32.43%) mandibles with retromandibular foramina. The population analyzed showed a high incidence of the retromolar foramen, especially on the right side. The identification of this anatomical variation helps in the planning of oral surgeries and during anesthetic procedures.
磨牙后孔和磨牙后管也是发生在磨牙后三角区的下颌骨解剖变异。该孔和管包含神经血管结构,为下臼齿和颊部区域提供额外的神经支配。本研究旨在确定伯南布哥联邦大学当代骨收藏馆下颌骨磨牙后孔的发生率和性别差异。对61个干燥下颌骨进行了分析,其中37个来自雄性,24个来自雌性。在61个下颌骨中,18个有磨牙后孔(29.5%),12个有单侧孔(19.67%)。男性下颌骨的发病率高于女性,其中12个(32.43%)下颌骨有下颌后孔。分析的人群显示磨牙后孔的发生率很高,尤其是在右侧。这种解剖变异的识别有助于口腔手术和麻醉过程的规划。
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引用次数: 0
Caracterização molecular de Prosopis juliflora (Sw.) DC. por meio de marcadores moleculares e índices de similaridade genética 朱氏Prosopis juliflora (Sw.)的分子特征特区。通过分子标记和遗传相似性指数,对其进行分类
Pub Date : 2020-03-13 DOI: 10.5007/2175-7925.2020.e70282
Messulan Rodrigues Meira, L. H. Santos, Quirlian Queite Araújo Anjos, Brenda Emilly Ferreira Santos, Leonardo de Sousa Guimarães, Carlos Bernard Moreno Cerqueira Silva
Prosopis juliflora (Sx.) DC., commonly known as algarroba, is an adapted tree species in Brazil with animal food value and environmental value in reforestation. However, little is known about its molecular genetic diversity. Therefore, the aim of our study was to use different similarity measures based on the genetic characterization of algarroba obtained through ISSR and RGA markers in naturalized individuals in southwestern Bahia. Young leaves of 36 algarroba individuals were collected in the municipality of Itapetinga, Bahia. DNA was extracted by the 10% SDS protocol and quantified by the absorbance ratio A260/A230 and A260/A280 nm. Ten pairs of RGA and 23 primers of ISSR were used for PCR, and the amplified products were electrophoresed on 2% agarose gels for 2 h. The genotyping results (binary matrix) were used to calculate the Jaccard, Simple Matching and Sorensen Dice similarity coefficients. Finally the UPGMA clustering, Tocher optimization and PCA were performed. We observed for ISSR and RGA 205 and 68 marks, respectively, with expected heterozygosity of 0.28 and 0.21; polymorphic information content was 91.71 and 0.17% and genetic distance 8.0 and 8.53, respectively. These findings contribute to our understanding of the wide distribution and adaptation of this species in reforested areas.
Prosopis juliflora(Sx.)DC。,通常被称为algaroba,是巴西的一种适应性树种,具有动物食物价值和重新造林的环境价值。然而,人们对其分子遗传多样性知之甚少。因此,我们研究的目的是基于通过ISSR和RGA标记在巴伊亚西南部归化个体中获得的algaroba的遗传特征,使用不同的相似性度量。在巴伊亚州伊塔佩廷加市采集了36株Algaroba个体的幼叶。通过10%SDS方案提取DNA,并通过吸光度比A260/A230和A260/A280nm进行定量。使用10对RGA和23对ISSR引物进行PCR,扩增产物在2%琼脂糖凝胶上电泳2小时。使用基因分型结果(二元矩阵)计算Jaccard、Simple Matching和Sorensen-Dice相似系数。最后进行UPGMA聚类、Tocher优化和主成分分析。我们观察到ISSR和RGA分别为205和68个标记,预期杂合度分别为0.28和0.21;多态性信息含量分别为91.71%和0.17%,遗传距离分别为8.0和8.53。这些发现有助于我们理解该物种在重新造林地区的广泛分布和适应。
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Biotemas
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