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Genetic stability in interspecific hybridizations of wheat populations determined by meiotic index and pollen viability 小麦群体种间杂交的遗传稳定性由减数分裂指数和花粉活力决定
Pub Date : 2021-06-01 DOI: 10.5007/2175-7925.2021.E79666
Patricia Frizon, S. Brammer, C. C. Deuner, A. Chechi, M. I. Lima, P. Scheeren
The objective of this study was to evaluate genetic stability by determining the meiotic index (MI) and pollen viability in F1RC2 segregating populations, obtained by the crosses between wheat commercial cultivars and synthetic wheat, aimed at introgression/genetic resistance. For MI, three ears were collected per genotype, before anthesis, and they were fixed in Carnoy’s solution. The cytological slides were prepared by crushing the anthers and staining with 1% acetocarmine, and the normal tetrads and the ones with micronuclei were observed under a light microscope. For pollen viability, the ears were collected at the stage of mature pollen grain. The methodological procedure was similar to that of the tetrads, evaluating the pollen grains as viable, with little starch, with two pores, different sizes and non-viable. The pollen grain size was measured by the Axion Vision software. All the segregating populations with MI above 90% and high pollen viability (above 85%) reflected a stable meiotic behavior. About the variation in size, the CIGM90.909/BRS 179 cross showed pollen grains with 58.43 μm and CIGM93.298/BRS Guamirim, 47.15 μm. According to the results, wheat segregating populations were considered meiotically stable and suitable for use in wheat breeding programs and for incorporation of new important genes.
本研究通过测定小麦商品品种与合成小麦杂交获得的F1RC2分离群体的减数分裂指数(MI)和花粉活力,评价其遗传稳定性。对于MI,每个基因型在开花前收集3个穗,并将它们固定在卡诺伊溶液中。粉碎花药,1%乙酰胭脂红染色,制作细胞学切片,光镜下观察正常四分体和微核四分体。为了测定花粉活力,在花粉粒成熟阶段采集穗。方法程序与四分体相似,评价花粉粒为有活力、淀粉含量少、有两个孔、大小不同、无活力。用Axion Vision软件测量花粉粒度。MI > 90%、花粉活力> 85%的分离群体均表现出稳定的减数分裂行为。在大小变异方面,CIGM90.909/BRS 179杂交的花粉粒大小为58.43 μm, CIGM93.298/BRS瓜米林的花粉粒大小为47.15 μm。结果表明,小麦分离群体减数稳定,适合用于小麦育种计划和引入新的重要基因。
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引用次数: 1
Ictiofauna do Parque Nacional da Serra da Bodoquena, Mato Grosso do Sul, Brasil: composição e subsídios à conservação 巴西南马托格罗索Serra da Bodoquena国家公园的鱼类:组成和保护补贴
Pub Date : 2021-06-01 DOI: 10.5007/2175-7925.2021.E76255
M. E. A. Vilela, Francisco de Paula Severo Neto, Fernando R. Carvalho, Otávio Froehlich
The Serra da Bodoquena includes the headwaters of important rivers of the upper Paraguay River basin and constitutes one of the more extensive karst formations in Brazil. The region is an important tourist destination and Serra da Bodoquena National Park (SBNP) was established in 2000. In this work, we provide a list of the ichthyofauna and describe the main water systems in SBNP. Surveys were carried out in August, September and December 2005 using trawl nets, gill nets and electrofishing. For the list of species, we included records made in subsequent studies, resulting in 60 species belonging to seven orders and 21 families, which are all autochthonous to the upper Paraguay River, except the tilapia Coptodon rendalli, the only non-native species. The species richness was higher in the Salobra River basin (49 species, 39 exclusive) compared to the Perdido River basin (15 species, six exclusive). We documented seven endemic species in the Serra da Bodoquena, of which two are threatened with extinction (Vulnerable category): Trichomycterus dali and Ancistrus formoso. As mitigation and preventive measures for environmental impacts, we suggest regularizing land use and increasing environmental education programs as ways of contributing to public policies that are involved in the conservation of aquatic systems and their biotic communities.
博多奎纳山脉包括巴拉圭河上游流域重要河流的源头,是巴西境内最广泛的喀斯特地貌之一。该地区是一个重要的旅游目的地,Serra da Bodoquena国家公园(SBNP)成立于2000年。在这项工作中,我们提供了一份鱼类区系的清单,并描述了SBNP的主要水系统。调查于2005年8月、9月和12月使用拖网、刺网和电钓进行。在物种列表中,我们纳入了随后的研究记录,得到了60种,隶属于7目21科,除Coptodon rendalli罗非鱼(Coptodon rendalli)是唯一的非本地物种外,所有物种都是巴拉圭河上游的土生物种。物种丰富度在萨洛布拉河流域(49种,39种专有)高于Perdido河流域(15种,6种专有)。本文记录了Bodoquena山的7种特有物种,其中2种濒临灭绝(易危类别):大理Trichomycterus dali和Ancistrus formoso。作为环境影响的缓解和预防措施,我们建议规范土地使用和增加环境教育计划,作为参与保护水生系统及其生物群落的公共政策的方式。
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引用次数: 1
Floristic composition of Melastomataceae between reforested and natural areas in the Recife Atlantic Forest, Pernambuco, Brazil 巴西伯南布哥累西腓大西洋森林中重新造林和自然区域之间的野牡丹科植物区系组成
Pub Date : 2021-03-04 DOI: 10.5007/2175-7925.2021.E77751
J. Maciel, Yuri de Souza Vieira Couceiro
Local extinction of native Atlantic Forest flora species occurs mainly due to habitat fragmentation. Reforestation and natural regeneration can mitigate these factors. The present study attempts to understand whether floristic composition and dispersal processes are being reestablished in an area of lowland Atlantic Forest, eight years after its reforestation. The study area was an old pasture set between two urban fragments in the city of Recife that was reforested in 2011. Melastomataceae species were collected in this area and the morphology of their fruits was compared with the species of the family recorded in the adjacent fragments. Miconia prasina, M. albicans, M. affinis, Clidemia hirta, and C. capitellata have been found in the reforested area. Although there are species with larger fruits in the adjacent fragments, there was no statistically significant difference between the means of the two areas. The fruit morphology indicates that the species found in the area are dispersed by birds and small mammals. It is possible to conclude that the reforested area already presents the expected ecological processes for a corridor between two fragments. Thus, the two adjacent areas serve as vectors for the colonization of native Atlantic Forest species in the reforested area.
本地大西洋森林植物物种的局部灭绝主要是由于栖息地的破碎化。重新造林和自然再生可以缓解这些因素。本研究试图了解大西洋低地森林重新造林八年后,该地区的植物区系组成和扩散过程是否正在重建。研究区域是位于累西腓市两个城市碎片之间的一片旧牧场,于2011年重新造林。在该地区采集了野牡丹科的物种,并将其果实的形态与相邻碎片中记录的科的物种进行了比较。在重新造林的地区发现了白念珠菌、白色念珠菌、亲和分枝杆菌、毛唇菌和头状毛唇菌。尽管在相邻的碎片中有果实较大的物种,但两个区域的平均值之间没有统计学上的显著差异。果实形态表明,该地区发现的物种以鸟类和小型哺乳动物为主。可以得出结论,重新造林的区域已经呈现出两个片段之间走廊的预期生态过程。因此,这两个相邻区域是大西洋原生森林物种在重新造林区域定居的载体。
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引用次数: 0
Otimização das condições de cultivo do fungo filamentoso Fusarium sp. EA 1.3.7 para a produção de xilanases 丝状真菌Fusarium sp.EA1.3.7产木聚糖酶培养条件的优化
Pub Date : 2021-03-04 DOI: 10.5007/2175-7925.2021.E77440
Eloísa Alves Nogueira, Tarcisio Michael Ferreira Soares De Oliveira, Ingrid Cristina Santos Amorim, Tássio Britto De Oliveira, D. L. Nelson, Vivian Machado Benassi
Xylanases are enzymes widely used in industrial processes, such as paper bleaching, the bakery industry, juice and beer clarification, malting, and the production of biofuels, due to the benefits and advantages of the biological catalyst. In this context, we aimed to bioprospecting filamentous fungi, select a potential xylanase-producing fungus, optimize the enzymatic production, and perform the biochemical characterization of the enzyme. Eleven filamentous fungi with distinct morphology were isolated from three samples of organic materials collected in the soil of the city of Diamantina, Minas Gerais, Brazil, and Fusarium sp. EA 1.3.7 was selected as a potential xylanase-producer. The best growth conditions for the production of xylanase were standardized in a submerged CP medium, for six days, at 30oC, using peptone as a nitrogen source, and wheat bran as a carbon source; the xylanases produced showed greater activities at 52oC and pH 5.3.
木聚糖酶是一种广泛应用于工业过程中的酶,如纸张漂白、烘焙工业、果汁和啤酒澄清、麦芽制备以及生物燃料生产,这是由于生物催化剂的优点和优势。在此背景下,我们旨在对丝状真菌进行生物勘探,选择一种潜在的产木聚糖酶的真菌,优化酶的生产,并对该酶进行生化表征。从巴西米纳斯吉拉斯州迪亚曼蒂纳市和镰刀菌属的土壤中收集的三个有机材料样品中分离出11种具有不同形态的丝状真菌。EA 1.3.7被选为潜在的木聚糖酶生产商。以蛋白胨为氮源,麦麸为碳源,在30℃的浸入式CP培养基中,对木聚糖酶的最佳生长条件进行了标准化,培养时间为6天;所产生的木聚糖酶在52℃和pH 5.3时表现出更高的活性。
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引用次数: 2
Evaluation of original Roundup® effects on Biomphalaria glabrata (Say, 1818) Roundup®对光滑生物阴茎的原始影响评估(Say,1818)
Pub Date : 2021-03-04 DOI: 10.5007/2175-7925.2021.E77320
Marta Júlia Faro, V. S. Moura, R. Augusto, M. Vasconcellos
The objective of this work is to evaluate the action of original Roundup® on the snail Biomphalaria glabrata infected and not infected by Schistosoma mansoni by observing its mortality and reproductive biology. Snails were submitted for 30 days to aqueous solutions of original Roundup at concentrations of 0.1, 0.09, 0.08, 0.07, 0.06, 0.05, 0.04, 0.03, 0.02, 0.018, 0.016, 0.014, 0.012 and 0.01 (%) to determine LC50. To analyze the reproductive activity of B. glabrata, against the pesticide, four B. glabrata groups were formed: infected and exposed to the pesticide; infected and not exposed (control); not infected and exposed; and not infected and not exposed(control). LC50 (0.012% aqueous solution of original Roundup) caused mortality of at most up to 95% for 24, 48 and 72 h during the experimental period (30 days), and lost effect totally after 27 days. The reproductive activity of the non-infected and exposed snails was 30.3 ± 20.5 eggs in 2.7 ± 1.2 egg masses, and there was a reduction of 51% in the average number of hatched snails (11.5 ± 7.5) in comparison to the control group (23.9 ± 18.0) for four weeks (p = 0.003). The results indicate that the lethal concentration (LC50) of the 0.012% aqueous solution of original Roundup has a significant action on Biomphalaria glabrata for up to 27 days. Roundup significantly diminished the fertility of infected and exposed snails in comparison with the control group.
本研究旨在通过观察曼氏血吸虫感染和未感染的光秃螺的死亡率和生殖生物学特性,评价草甘膦除草剂农达对光秃螺的作用。将钉螺置于浓度为0.1、0.09、0.08、0.07、0.06、0.05、0.04、0.03、0.02、0.018、0.016、0.014、0.012和0.01(%)的农达原液水溶液中30 d,测定LC50。为了分析光斑小蠊对农药的生殖活性,将其分为4个群体:感染和暴露于农药;已感染但未暴露(对照);未受感染和暴露;没有感染也没有暴露(对照)在试验期内(30 d), LC50(0.012%原农达水溶液)在24、48和72 h的致死率最高可达95%,27 d后致死率完全消失。未感染和暴露的钉螺在4周内的平均孵化数(11.5±7.5)只比对照组(23.9±18.0)只减少了51% (p = 0.003),在2.7±1.2个卵团中有30.3±20.5个卵。结果表明,0.012%农达原液对光斑生物phalaria glabrata的致死浓度(LC50)可达27 d。与对照组相比,农达显著降低了受感染和暴露的蜗牛的生育能力。
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引用次数: 0
Análise comparativa de protocolos para extração de DNA genômico em Prosopis juliflora (Sw.) DC 细叶Prosopis juliflora(Sw.)DC基因组DNA提取方案的比较分析
Pub Date : 2021-03-04 DOI: 10.5007/2175-7925.2021.E76552
L. H. Santos, Cibelle Santos Dias, Jardyelle Carvalho Lima, Messulan Rodrigues Meira, C. B. M. Cerqueira-Silva
The objective of the present research was to compare the efficiency among seven protocols adapted for the extraction of genomic DNA from the species Prosopis juliflora (Sw.) DC. The protocols used were based on their buffers: SDS 10%, CTAB 5%, Sorbitol, CTAB 3% and Sorbitol, SDS 0.7% and NaCl, CTAB 2%, CTAB 2% and NaCl 1.4M. In all tests, the use of β-mercaptoethanol, liquid nitrogen, proteinase K and RNAses was eliminated. The samples consisted of leaf primordia in each specimen in triplicate. The quantity and quality of the extracted DNA were evaluated by electrophoresis in 1% agarose gel and by reading the absorbances in a spectrophotometer. For amplification reactions, the ISSR primer was used. Among the tested extraction buffers, SDS 10%, CTAB 5% and CTAB 3%, and Sorbitol were the most efficient for P. juliflora. Of these three protocols, CTAB 3% and Sorbitol showed a better level of purity despite the lower amount of DNA (123 ng/µL) compared to 10% SDS and 5% CTAB buffers (312 and 321 ng/µL, respectively, and high content of contaminants). We conclude that it is possible to use extraction protocols without additives that are harmful to human health and at a lower cost in the extraction process.
本研究的目的是比较适用于从Prosopis juliflora(Sw.)DC中提取基因组DNA的七种方案的效率。使用的方案基于它们的缓冲液:SDS 10%,CTAB 5%,山梨醇,CTAB 3%和山梨醇,SDS 0.7%和NaCl,CTAB 2%,CTAB 2%和NaCl 1.4M。在所有测试中,消除了β-巯基乙醇、液氮、蛋白酶K和RNA酶的使用。样品由每个样品中的叶原基组成,一式三份。提取的DNA的数量和质量通过在1%琼脂糖凝胶中的电泳和通过在分光光度计中读取吸光度来评估。对于扩增反应,使用ISSR引物。在所测试的提取缓冲液中,SDS 10%、CTAB 5%和CTAB 3%以及山梨醇对菊芋的提取效率最高。在这三种方案中,尽管与10%SDS和5%CTAB缓冲液(分别为312和321 ng/ml,污染物含量高)相比,DNA含量较低(123 ng/ml),但3%的CTAB和山梨醇显示出更好的纯度。我们得出的结论是,在提取过程中使用不含对人体健康有害且成本较低的添加剂的提取方案是可能的。
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引用次数: 0
Diversidade das pescarias realizadas com rede de emalhar em um estuário tropical do nordeste do Brasil 巴西东北部热带河口刺网渔业的多样性
Pub Date : 2021-03-04 DOI: 10.5007/2175-7925.2021.E77545
Severino Adriano de Oliveira Lima, H. A. Andrade, Marcondes Agostinho Gonzaga Junior, Donovan Filipe Henrique Pinto
The aim of the present research was to study the diversity of the ichthyofauna captured with the most commonly used gillnet in the state of Pernambuco. In total, 14 collections were made in the Santa Cruz Canal (CSC) between July 2013 and June 2014. The samples were collected using a 30 mm mesh gillnet. The captured fish were removed and separated in margin and center positions, as well as in the net’s vertical position (upper, middle, and lower). Concerning frequency of occurrence, the species were classified as common, accessory, or occasional. Alpha and beta diversity analyses were performed. In total, 19 species were captured, with Opisthonema oglinum and Anchovia clupeoides being the only constants. The estimated alpha diversities did not show significant differences in the temporal and spatial strata. In the beta diversity, two groups were distinguished, with the largest generally occurring in large abundances of O. oglinum. The evaluated net was efficient for the target specie O. oglinum, while the catches of other species corresponded to less than 13% of the icitiofauna already found in the CSC. Therefore, management measures that aim to restrict the use of fishing gear for the protection of diversity considering the seasonality and spatiality in the channel is not recommended.
本研究的目的是研究在伯南布哥州最常用的刺网捕获的鱼系动物的多样性。2013年7月至2014年6月期间,在圣克鲁斯运河(CSC)共进行了14次收集。样品采集采用30毫米刺网。捕获的鱼被移走,并在边缘和中心位置分开,以及在网的垂直位置(上、中、下)。根据发生的频率,这些物种被分为常见、辅助和偶尔。进行α和β多样性分析。共捕获鱼种19种,不变的有油鱼(Opisthonema oglinum)和凤尾鱼(Anchovia clupeides)。估算的α多样性在不同时空层间没有显著差异。在β多样性中,可以区分出两个群体,其中最大的群体通常出现在O. oglinum的大量丰度中。所评估的网对目标物种O. oglinum是有效的,而其他物种的渔获量对应于CSC中已发现的城市动物的不到13%。因此,考虑到航道的季节性和空间性,不建议采取旨在限制渔具使用以保护多样性的管理措施。
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引用次数: 0
Vascular epiphytic component of “Brejo de altitude” in northeastern Brazil: floristic composition and phytosociological structure 巴西东北部“布雷霍高原”的维管附生成分:区系组成和植物社会学结构
Pub Date : 2021-03-04 DOI: 10.5007/2175-7925.2021.E76492
R. G. Dias-Terceiro, V. S. Gomes, M. Menezes, L. O. Demarchi, J. Fabricante, M. B. Albuquerque
The Brazilian Northeastern Brejos is an important area of biodiversity in the Atlantic Forest. However, little is known about the species composition of epiphytes in this type of vegetation. The present study aims to characterize the floristic and phytosociological structure of vascular epiphytic components in an area of the Brazilian Northeastern Brejo. All epiphytes occurring in the area were collected and compared with other studies through NMDS analysis. For the epiphyte phytosociological structure, sixty 10 × 10 m sampling plots were installed. The floristic survey of epiphytes recorded 23 species, distributed in 19 genera and ten families. In plots, 65 host individuals were sampled, and the occurrence of 197 vascular epiphytes, distributed in 12 species, 12 genera and five families was recorded. The floristic composition differs from other areas of the Atlantic Forest, including the northeast, making the Brejo a very peculiar area and with the utmost need of a plan for the conservation of these species.
巴西东北部布雷霍斯是大西洋森林生物多样性的重要地区。然而,对这类植被中附生植物的种类组成知之甚少。本研究旨在描述巴西东北部布雷霍地区维管附生成分的区系和植物社会学结构。收集了该地区所有附生植物,并通过NMDS分析与其他研究进行了比较。对于附生植物的植物社会学结构,设置了60个10 × 10 m样地。附生植物区系调查记录到23种,隶属于10科19属。样地共采集寄主个体65个,记录维管附生植物197种,分属5科12属12种。这里的植物区系组成不同于大西洋森林的其他地区,包括东北部,这使得布雷霍成为一个非常特殊的地区,迫切需要一个保护这些物种的计划。
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引用次数: 1
A survey of Calliphoridae and Mesembrinellidae (Diptera) in semideciduous seasonal forest, Paraná, Brazil 巴西巴拉那半熟季节性森林中丽蝇科和夜蛾科的调查
Pub Date : 2021-03-04 DOI: 10.5007/2175-7925.2021.E77073
Alex Sandro Barros de Souza, Marcos Magalhães de Souza, Patrícia Pereira Gomes, Jean Victor Nery da Silva, Giuliano Kaulfuss Bertinoti, José Adolfo Mota de Almeida
Calliphoridae and Mesembrinellidae include Diptera species that are good indicators of changes in forest environments. The present work aimed to inventory the Calliphoridae and Mesembrinellidae in Ilha Grande National Park and correlate the abundance of these organisms with environmental variables. Adult dipterans were collected during two expeditions in 2019 using two sampling methods, a Malaise trap and baited traps, distributed on two islands in the park. During the study, 1,007 individuals of 12 species of Calliphoridae and four species of Mesembrinellidae were collected. Family Calliphoridae was more abundant (97.12%). The abundance of native species showed a strong negative correlation with the abundance of the genus Chrysomya. Rodrigues Island (Island I) had higher diversity levels and no species of Chrysomya were recorded in this area, which may indicate a greater degree of environmental preservation. The results indicate that human action in natural environments can negatively impact species diversity, as found in Ilha Grande National Park that, historically, was partially occupied and is still in the process of regeneration.
丽蝇科和膜蝇科包括能很好地反映森林环境变化的直翅目昆虫。目前的工作旨在清点伊尔哈格兰德国家公园的丽蝇科和Mesenbrinellidae,并将这些生物的丰度与环境变量联系起来。在2019年的两次探险中,使用了两种采样方法,即Malaise诱捕器和诱饵诱捕器,收集了成年Dipters,分布在公园的两个岛屿上。在研究过程中,共采集了12种丽蝇科和4种中膜蝇科的1007只个体。丽蝇科的数量较多(97.12%),本地种的数量与金蝇属的数量呈极强的负相关。罗德里格斯岛(I岛)的多样性水平较高,该地区没有记录到金龟子属物种,这可能表明环境保护程度更高。研究结果表明,人类在自然环境中的行为会对物种多样性产生负面影响,正如在历史上被部分占用并仍处于再生过程中的伊尔哈格兰德国家公园所发现的那样。
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引用次数: 2
Avaliação da inflamabilidade de espécies nativas do cerrado 塞拉多本地物种可燃性评价
Pub Date : 2020-12-09 DOI: 10.5007/2175-7925.2020.e74323
Marcileia Dias de Oliveira, Josué Luiz Marinho Junior, Ângela Helena Silva Mendes Stival, Bárbara Gomes Ferreira, Francisca de Cássia Silva da Silva, Marcos Vinicius Giongo Alves
Na ocorrencia de incendios florestais, a propagacao se deve principalmente as caracteristicas das folhas. De certa forma, as folhas funcionam como potencializador ou retardador da propagacao de incendios, ja que as que possuem baixa inflamabilidade podem servir como barreiras de protecao contra incendios. O presente trabalho teve por finalidade, analisar a inflamabilidade de 6 especies do cerrado. Para a realizacao do experimento, foi utilizado o epirradiador com uma faixa de temperatura controlada entre 630 a 650 oC. Das especies avaliadas foram coletadas 50 amostras de 1g (± 0,1 g) de cada especie. Foram realizadas 50 repeticoes de queima, na qual se pode analisar o Tempo para Ignicao (TI), Frequencia de ignicao (FI), Duracao de Combustao (DC), Indice de Combustao (IC) e Valor de Inflamabilidade (VI). Os valores de inflamabilidade das especies foram: Protium Heptaphyllum igual a 1 (fracamente inflamavel); Caryocar brasiliense Cambess., Byrsonima verbascifolia (L.) DC., Acacia polyphylla e Terminalia argentea Mart. iguais a 3 (inflamaveis); e a especie Lafoensia pacari igual a 4 (altamente inflamavel). Percebeu-se que o teor de umidade das especies nao teve correlacao com os valores de inflamabilidade e que outros fatores, como presenca de oleos, resinas e gomas vegetais podem influenciar no processo de combustao.
在森林火灾发生时,传播主要是由于叶子的特性。在某种程度上,叶子可以增强或减缓火灾的传播,因为那些可燃性低的叶子可以作为防火屏障。本研究旨在分析塞拉多6种植物的易燃性。为了进行实验,使用了温度控制在630 - 650℃之间的热散热器。从评估的物种中采集50个样本,每个物种1g(±0.1 g)。50 repeticoes进行燃烧,在分析Ignicao(你的时间),点火频率(FI),长度的Combustao (DC),可燃性Combustao指数(ci)和价值(VI),耐燃性物种的价值观:氕Heptaphyllum等于1(弱inflamavel);carycar brasiliense Cambess。毛蕊花Byrsonima verbascifolia (L.)特区。= =地理= =根据美国人口普查,这个县的总面积是,其中土地和(3.064平方公里)水。等于3(易燃);e种Lafoensia pacari等于4(高度易燃)。我们注意到,物种的水分含量与可燃性值没有相关性,其他因素,如油、树脂和植物胶的存在可能影响燃烧过程。
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引用次数: 0
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Biotemas
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