{"title":"Prediction of MicroRNAs bind to Toll-like Receptors Pathway in Chicken based on Bioinformatics Method","authors":"Maryam Gholizade, J. Fayazi, S. Rahimnahal","doi":"10.32598/rmm.7.4.33","DOIUrl":"https://doi.org/10.32598/rmm.7.4.33","url":null,"abstract":"","PeriodicalId":30778,"journal":{"name":"Research in Molecular Medicine","volume":"7 1","pages":"33-42"},"PeriodicalIF":0.0,"publicationDate":"2019-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48929881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nasrin Zarkar, Mohammad Ali Nasiri Khalili, S. Khodadadi, M. Zeinoddini, Maryam Ghodrati Siahmazgi, N. Faramarzi
BackgroundDAB389IL-2 is considered a fusion immunotoxin and it is used for the CTCL therapy. DAB389IL-2 includes of two distinct portions; the catalytic domain of diphtheria toxin and IL-2. DAB389IL-2 duo to the presence of a free cysteine residue (Cys 513 in IL-2 part) is prone to unwanted intramolecular and intermolecular disulfide bonds formation and aggregation problems. Aggregation is considered as the most common physical instability. PEGylation is an effective approach to increase the stability and half-life of therapeutic proteins.Materials and methodsIn this study, the PEGylation of recombinant DAB389IL-2 was performed by mPEG-vinylsulfone, through partial denaturation condition at 4 0C for 24 h. To confirm the PEGylation reaction, SDS-PAGE and Dynamic Light Scattering (DLS) was used. The structure of DAB389IL-2 and PEGylated immunotoxin DAB389IL-2 was analyzed using the circular dichroism (CD) and fluorescence methods. Also, K562 cells line were treated with various concentrations of DAB389IL-2 and conjugated form. In the following, the nuclease activity of DAB389IL-2 and PEGylated form was determined.ResultsThe SDS-PAGE result confirmed the site-specific PEGylation of DAB389IL-2. Spectroscopy results exhibited that the PEGylation doesn’t affect the protein native structure. Also, cytotoxicity assay and nuclease activity test confirmed that PEGylated protein induces death in K562 cells line and DNA degradation respectively. ConclusionIt is concluded that the PEGylated immunotoxin DAB389IL-2 has a proper structure and function; thus, PEGylated immunotoxin requires more survey due its unique properties.
{"title":"Site-Specific PEGylation of Recombinant Immunotoxin DAB389IL-2: Structural and Functional Assessment","authors":"Nasrin Zarkar, Mohammad Ali Nasiri Khalili, S. Khodadadi, M. Zeinoddini, Maryam Ghodrati Siahmazgi, N. Faramarzi","doi":"10.32598/RMM.7.4.7","DOIUrl":"https://doi.org/10.32598/RMM.7.4.7","url":null,"abstract":"BackgroundDAB389IL-2 is considered a fusion immunotoxin and it is used for the CTCL therapy. DAB389IL-2 includes of two distinct portions; the catalytic domain of diphtheria toxin and IL-2. DAB389IL-2 duo to the presence of a free cysteine residue (Cys 513 in IL-2 part) is prone to unwanted intramolecular and intermolecular disulfide bonds formation and aggregation problems. Aggregation is considered as the most common physical instability. PEGylation is an effective approach to increase the stability and half-life of therapeutic proteins.Materials and methodsIn this study, the PEGylation of recombinant DAB389IL-2 was performed by mPEG-vinylsulfone, through partial denaturation condition at 4 0C for 24 h. To confirm the PEGylation reaction, SDS-PAGE and Dynamic Light Scattering (DLS) was used. The structure of DAB389IL-2 and PEGylated immunotoxin DAB389IL-2 was analyzed using the circular dichroism (CD) and fluorescence methods. Also, K562 cells line were treated with various concentrations of DAB389IL-2 and conjugated form. In the following, the nuclease activity of DAB389IL-2 and PEGylated form was determined.ResultsThe SDS-PAGE result confirmed the site-specific PEGylation of DAB389IL-2. Spectroscopy results exhibited that the PEGylation doesn’t affect the protein native structure. Also, cytotoxicity assay and nuclease activity test confirmed that PEGylated protein induces death in K562 cells line and DNA degradation respectively. ConclusionIt is concluded that the PEGylated immunotoxin DAB389IL-2 has a proper structure and function; thus, PEGylated immunotoxin requires more survey due its unique properties.","PeriodicalId":30778,"journal":{"name":"Research in Molecular Medicine","volume":"7 1","pages":"7-16"},"PeriodicalIF":0.0,"publicationDate":"2019-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46460565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. E. A. Komi, M. Sadeghi-shabestari, Dariush Shanebandi, Z. Babaloo, A. Razavi, S. Sadigh-Eteghad, Tohid Kazemi
{"title":"Investigation of Chitinase3like-1 (Chiti3L1) Gene Polymorphism (rs4950928) with Susceptibility to Allergic Asthma in Iranian Northwestern Azeri Population","authors":"D. E. A. Komi, M. Sadeghi-shabestari, Dariush Shanebandi, Z. Babaloo, A. Razavi, S. Sadigh-Eteghad, Tohid Kazemi","doi":"10.32598/RMM.7.4.17","DOIUrl":"https://doi.org/10.32598/RMM.7.4.17","url":null,"abstract":"","PeriodicalId":30778,"journal":{"name":"Research in Molecular Medicine","volume":"7 1","pages":"17-24"},"PeriodicalIF":0.0,"publicationDate":"2019-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46827232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Acute Beetroot Juice Intake: Hematological, Antioxidant and Lipid Parameters in Female Athletes","authors":"M. Lotfi, M. Azizi, W. Tahmasebi, P. Bashiri","doi":"10.18502/rmm.v7i1.5259","DOIUrl":"https://doi.org/10.18502/rmm.v7i1.5259","url":null,"abstract":"<jats:p>.</jats:p>","PeriodicalId":30778,"journal":{"name":"Research in Molecular Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41656649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Amouzeshi, S. Z. Moossavi, Seyed Yoosef Javad Moosavi, M. Zardast, M. Malekaneh, S. Esmaili, Abdolghader Taneh, Nasim Lotfi, M. Moossavi, R. Hoshyar
.
{"title":"Aqueous Cannabis Extract Prevents Ethylene Glycol-induced Renal Calcium Crystallization","authors":"A. Amouzeshi, S. Z. Moossavi, Seyed Yoosef Javad Moosavi, M. Zardast, M. Malekaneh, S. Esmaili, Abdolghader Taneh, Nasim Lotfi, M. Moossavi, R. Hoshyar","doi":"10.18502/rmm.v7i1.5255","DOIUrl":"https://doi.org/10.18502/rmm.v7i1.5255","url":null,"abstract":"<jats:p>.</jats:p>","PeriodicalId":30778,"journal":{"name":"Research in Molecular Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44967088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Abdi, Mitra Hosseinpour, Kazem Mashayekhi, M. Mousavi, Seyedeh Elham Badiee Kheirabadi, M. Sankian
.
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{"title":"Optimization of Cloning Conditions for High-level Production of Recombinant Mouse Interleukin-2 in Escherichia coli","authors":"A. Abdi, Mitra Hosseinpour, Kazem Mashayekhi, M. Mousavi, Seyedeh Elham Badiee Kheirabadi, M. Sankian","doi":"10.18502/RMM.V7I1.5256","DOIUrl":"https://doi.org/10.18502/RMM.V7I1.5256","url":null,"abstract":"<jats:p>.</jats:p>","PeriodicalId":30778,"journal":{"name":"Research in Molecular Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43554506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Moghimi, H. Sadeghi Tafti, Faezeh Namazi, Mansoor Salehi
.
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{"title":"Investigation of the Impact of Foretinib on AURKA and AURKB Expression in T98 Glioblastoma Cell Line","authors":"M. Moghimi, H. Sadeghi Tafti, Faezeh Namazi, Mansoor Salehi","doi":"10.18502/rmm.v7i1.5254","DOIUrl":"https://doi.org/10.18502/rmm.v7i1.5254","url":null,"abstract":"<jats:p>.</jats:p>","PeriodicalId":30778,"journal":{"name":"Research in Molecular Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45251071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Evaluation of RHOXF1 Gene Expression in Different Cell Lines","authors":"Shadi Madipour, Shahin Amiri, M. Modarressi","doi":"10.32598/RMM.7.3.9","DOIUrl":"https://doi.org/10.32598/RMM.7.3.9","url":null,"abstract":"","PeriodicalId":30778,"journal":{"name":"Research in Molecular Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42825769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohsen Sheykhhasan, Hamed Manoochehri, Pouria Samadi, R. Fotouhi-Ardakani, N. Kalhor
The rotator cuff injury introduces as significant damage of trauma and others factors, and currently recognized as a prevalent orthopedic problem. Thus, many clinical procedures have been demonstrated to reduce the complication of rotator cuff injury with varying degrees of success. Platelet-Rich Plasma (PRP) is being enchantingly agreed as one of the most effectiveness strategies for the symptomatic treatment of rotator cuff injury. Many confusing parameters can influence the result of PRP including size of rotator cuff injury, patient age, PRP producing strategy, and number and timing of PRP injections. However, there is some evidences which support beneficial effect PRP in term relieving pain and restoring function, along with minimal adverse effect, in compared with corticosteroids, and other nonsurgical methods. Besides platelet-rich plasma, Mesenchymal Stem Cells (MSCs) are another biological alternative that can be used to treat rotator cuff injuries. Due to lack of large clinical trials applicability, these biological cells have challenges fairly similar to PRP. In conclusion, there is a need for further research to understand the potential application of both of these alternatives as a safe and effective therapeutic option for rotator cuff injuries. A B S T R A C T
肩袖损伤作为创伤等因素的重要损伤引入，是目前公认的骨科普遍存在的问题。因此，许多临床手术已被证明可以减少肩袖损伤的并发症，并取得了不同程度的成功。富血小板血浆（PRP）被公认为是治疗肩袖损伤最有效的治疗策略之一。许多令人困惑的参数会影响PRP的结果，包括肩袖损伤的大小、患者年龄、PRP产生策略以及PRP注射的次数和时间。然而，与皮质类固醇和其他非手术方法相比，有一些证据支持PRP在长期缓解疼痛和恢复功能方面的有益效果，以及最小的不良反应。除了富含血小板的血浆外，间充质干细胞（MSC）是另一种可用于治疗肩袖损伤的生物替代品。由于缺乏大型临床试验的适用性，这些生物细胞面临着与PRP相当相似的挑战。总之，有必要进行进一步的研究，以了解这两种替代方案作为肩袖损伤安全有效的治疗选择的潜在应用。A B S T R A C T
{"title":"Effect of Platelet-rich Plasma and Mesenchymal Stem Cells as Two Biological Alternatives in Rotator Cuff Injury Treatment: A Mini-review","authors":"Mohsen Sheykhhasan, Hamed Manoochehri, Pouria Samadi, R. Fotouhi-Ardakani, N. Kalhor","doi":"10.32598/RMM.7.3.1","DOIUrl":"https://doi.org/10.32598/RMM.7.3.1","url":null,"abstract":"The rotator cuff injury introduces as significant damage of trauma and others factors, and currently recognized as a prevalent orthopedic problem. Thus, many clinical procedures have been demonstrated to reduce the complication of rotator cuff injury with varying degrees of success. Platelet-Rich Plasma (PRP) is being enchantingly agreed as one of the most effectiveness strategies for the symptomatic treatment of rotator cuff injury. Many confusing parameters can influence the result of PRP including size of rotator cuff injury, patient age, PRP producing strategy, and number and timing of PRP injections. However, there is some evidences which support beneficial effect PRP in term relieving pain and restoring function, along with minimal adverse effect, in compared with corticosteroids, and other nonsurgical methods. Besides platelet-rich plasma, Mesenchymal Stem Cells (MSCs) are another biological alternative that can be used to treat rotator cuff injuries. Due to lack of large clinical trials applicability, these biological cells have challenges fairly similar to PRP. In conclusion, there is a need for further research to understand the potential application of both of these alternatives as a safe and effective therapeutic option for rotator cuff injuries. A B S T R A C T","PeriodicalId":30778,"journal":{"name":"Research in Molecular Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49259352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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