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Global Trends of Decarbonisation as a Determining Factor for the Development of External Economic Activity of Metallurgical Enterprises 全球脱碳趋势是冶金企业外部经济活动发展的决定因素
Q4 Social Sciences Pub Date : 2022-08-01 DOI: 10.2478/plua-2022-0008
N. Levchenko, G. Shyshkanova, G. Abuselidze, Yu. M. Zelenin, V. Prykhodko, M. Kovalskyi
Abstract The article states that in the conditions of climate crisis aggravation the decarbonisation of the economy acquires the status of a global initiative aimed at achieving zero CO2 emissions by 2050. It is emphasised that this initiative is closely accompanied by legislation, business modernisation programmes and the creation of equal conditions for both domestic producers and exporters of carbon-intensive products (in particular, through the introduction of the carbon border adjustment mechanism - CBAM) and new rules of the world market. The consequences of CBAM implementation into Ukrainian metallurgical enterprises and the development of their foreign economic activity are determined in the example of Metinvest Group (Ukraine) enterprises. The ambivalence of CBAM is proven. It is substantiated that CBAM implementation will not accelerate, but, on the contrary, will slow down the process of decarbonisation, which will eventually lead to the loss of domestic metallurgical enterprises’ competitive position in the European market of metal products. Regarding the need to take transitional measures to decarbonise the metallurgical business (first of all, to ensure proper investment), the decision should be based on the expected decoupling effect - positive dynamics of economic growth while reducing the negative impact on the environment. The estimating method of the decoupling effect has been modified according to the integrated approach. A trend analysis of the integrated decalin effect indicator and its components was performed in Metinvest Group companies (Ukraine). Based on the results of econometric analysis of the impact of investment on greenhouse gas emissions, the author’s model for determining the volume of investment in the process of decarbonisation of metallurgical enterprises for the period up to 2050 is proposed. Comparative analysis is performed regarding investment in the process of decarbonisation under the scenarios by GMK Center, IEA, McKinsey and Roland Berger, as well as the author’s model.
文章指出,在气候危机加剧的条件下,经济脱碳获得了旨在到2050年实现零二氧化碳排放的全球倡议的地位。会议强调,这一倡议与立法、商业现代化计划、为国内碳密集型产品生产商和出口商创造平等条件(特别是通过引入碳边界调整机制- CBAM)以及国际市场新规则密切相关。乌克兰冶金企业实施CBAM的后果及其对外经济活动的发展以Metinvest集团(乌克兰)企业为例确定。证明了CBAM的矛盾性。事实证明,CBAM的实施不仅不会加速,反而会减缓脱碳的进程,最终导致国内冶金企业在欧洲金属制品市场的竞争地位丧失。关于需要采取过渡措施使冶金业务脱碳(首先,确保适当的投资),决策应基于预期的脱钩效应-经济增长的积极动力同时减少对环境的负面影响。根据综合方法对解耦效果的估计方法进行了改进。对Metinvest集团(乌克兰)公司的综合十烷林效应指标及其组成部分进行了趋势分析。在对投资对温室气体排放影响的计量分析结果的基础上,提出了确定到2050年冶金企业脱碳过程投资规模的模型。对比分析了GMK中心、IEA、麦肯锡和罗兰贝格在不同情景下对脱碳过程的投资,以及作者的模型。
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引用次数: 0
Impact of Sowing Dates on the Variability of Different Traits of Fenugreek 播期对胡芦巴不同性状变异性的影响
Q4 Social Sciences Pub Date : 2022-08-01 DOI: 10.2478/plua-2022-0006
І. Bobos, I. Fedosiy, O. Zavadska, O. Komar, O. Tonkha, M. Furdyha, A. Rucins
Abstract The article presents the results of studies of two samples of fenugreek: blue (Trigonella coerulea (Desr.) Ser.) and hay (Trigonella foenum graecum L.) on the set of economic, biological and biochemical parameters. The researched samples of fenugreek have a wide range of variability of indicators depending on the terms of sowing and weather conditions. More rapid development was shown by fenugreek species on the summer sowing dates (III decade of June) with a vegetation period of 36–37 days. To obtain a high yield of green mass of fenugreek plants, the early spring (II–III decade of April) and late spring (I decade of May) sowing terms were more suitable. The obtained green mass yield was: 5.7–6.9 t∙ha–1 – blue fenugreek, and 7.3–9.3 t∙ha–1 – hay fenugreek, with the content of the dry matter being 12.4–28.4%, the sum of sugars 2.5–5.0% and vitamin C – 38.0–51.8 mg∙(100 g)−1. To obtain spices called “mushroom grass”, it is promising to use hay fenugreek with early spring (II–III decade of April) and late spring (I decade of May) terms of sowing, as a more developed vegetative mass is formed with the dry weight yield of plants 1.3–1.4 t∙ha–1. An increase in the amount of precipitation by 1 mm resulted in fluctuations of green mass yield from 15.0 to 77.3 kg∙ha–1 and dry mass yield from 0.693 to 25.9 kg∙ha–1.
摘要本文介绍了胡芦巴的两个样品:蓝色(Trigonella coerulea(Desr.)Ser.)和干草(Trigonella foenum graecum L.)对经济、生物和生化参数的影响。胡芦巴的研究样品具有广泛的指标变异性,这取决于播种条件和天气条件。胡芦巴物种在夏季播种期(6月的第三个十年)表现出更快的发育,植被期为36-37天。为了获得高产量的胡芦巴绿色植株,早春(4月的第二至第三个十年)和晚春(5月的第十年)的播种期更为合适。获得的绿色质量产量为:5.7–6.9 t∙ha–1–蓝色胡芦巴和7.3–9.3 t∙ha-1–干草胡芦巴,干物质含量为12.4–28.4%,糖的总和为2.5–5.0%,维生素C为38.0–51.8 mg∙(100 g)−1。为了获得被称为“蘑菇草”的香料,有希望在早春(4月的第二至第三个十年)和晚春(5月的第一个十年。降水量增加1毫米,导致绿色物质产量从15.0公斤/公顷波动到77.3公斤/公顷,干物质产量从0.693公斤/公顷变化到25.9公斤/公顷。
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引用次数: 0
Research Support Model for Improving the Effectiveness of Medical Study Data Collection 提高医学研究数据收集有效性的研究支持模型
Q4 Social Sciences Pub Date : 2022-08-01 DOI: 10.2478/plua-2022-0009
S. Bāliņa, Edgars Salna, I. Kojalo, Elīza Avotiņa
Abstract The paper describes the research support model for improving the effectiveness of the medical research data collection process and data quality. Every research project involves a data collection phase, during which different organisation, legal and technology factors are involved, including various procedures (questionnaire design, annotation, database design, data entry, data validation, discrepancy management, medical coding and data mining). The key task of clinical data management is to obtain high-quality data, which can be achieved by minimising data input errors and timely identifying missing data. This process is often time-consuming and takes up a significant part of the research project budget in both veterinary and human medicine. The aim of this study is to elaborate the research support model for the creation of a data collection automation software tool, which will allow one to ensure better data quality, shorten the time for data collection and minimise human work volume and respective human resource expenses, making research projects more effective in terms of their timing and budget. Research work included analysis of the current situation, its shortcomings, typical research project budget distribution and existing automated electronic data collection tools (EDC). Research was carried out in partnership with the Institute of Clinical and Preventive Medicine of the University of Latvia.
摘要本文描述了研究支持模型,以提高医学研究数据收集过程的有效性和数据质量。每个研究项目都涉及一个数据收集阶段,在此阶段涉及不同的组织、法律和技术因素,包括各种程序(问卷设计、注释、数据库设计、数据输入、数据验证、差异管理、医疗编码和数据挖掘)。临床数据管理的关键任务是获得高质量的数据,这可以通过最大限度地减少数据输入错误和及时识别缺失数据来实现。这一过程通常很耗时,并且占用了兽医和人类医学研究项目预算的很大一部分。本研究的目的是为创建数据收集自动化软件工具制定研究支持模型,这将使人们能够确保更好的数据质量,缩短数据收集时间,最大限度地减少人力工作量和相应的人力资源支出,使研究项目在时间和预算方面更加有效。研究工作包括分析现状、不足、典型的研究项目预算分配和现有的自动化电子数据收集工具(EDC)。这项研究是与拉脱维亚大学临床和预防医学研究所合作进行的。
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引用次数: 0
Present and Future Availability of Livestock Manure for Biogas Production in Latvia 拉脱维亚用于沼气生产的畜禽粪便的现在和未来的可用性
Q4 Social Sciences Pub Date : 2022-08-01 DOI: 10.2478/plua-2022-0001
A. Lagzdins
Abstract In order to address existing and foreseen climate change and environmental challenges, the European Commission has adopted a set of proposals as part of the European Green Deal to reduce emissions of greenhouse gases and become the first climate-neutral continent in the world. In the energy sector it is planned to implement specific actions to increase the contribution from renewable sources of energy. Livestock manure as a renewable resource can be processed to produce biogas through anaerobic digestion processes. This study aimed to estimate the amount of livestock manure potentially available for biogas production in Latvia based on statistical information on the number of animals representing current and past trends, and forecasts for the future provided by the results of the Latvian Agricultural Sector Analysis Model (LASAM). The results of this study showed that the share of livestock manure processed to produce biogas has increased considerably during the time period from 2010 to 2019 in all groups of livestock considered for this application in Latvia. However, there are still opportunities to increase the share of manure utilised for anaerobic digestion, especially in the livestock groups of dairy cows and non-dairy cows, as only 16% and 13% of the available amount of manure was processed in 2019, respectively. The LASAM results indicate that the number of animals will experience negligible changes or will decrease; therefore, more attention will need to be paid to increase the efficiency of processing the existing amount of manure rather than relying on an additional amount of manure available for biogas production.
摘要为了应对现有和预见到的气候变化和环境挑战,欧盟委员会通过了一系列提案,作为欧洲绿色协议的一部分,以减少温室气体排放,成为世界上第一个气候中立的大陆。在能源部门,计划采取具体行动,增加可再生能源的贡献。牲畜粪便作为一种可再生资源,可以通过厌氧消化过程生产沼气。本研究旨在根据代表当前和过去趋势的动物数量统计信息,以及拉脱维亚农业部门分析模型(LASAM)结果提供的未来预测,估计拉脱维亚可能用于沼气生产的牲畜粪便量。这项研究的结果表明,在2010年至2019年期间,拉脱维亚所有考虑采用这种方法的牲畜中,用于生产沼气的牲畜粪便的比例都大幅增加。然而,仍有机会增加用于厌氧消化的粪肥份额,特别是在奶牛和非奶牛的牲畜群体中,因为2019年分别只有16%和13%的可用粪肥被处理。LASAM结果表明,动物的数量将经历可忽略的变化或将减少;因此,需要更多地关注提高处理现有量的粪肥的效率,而不是依赖于可用于沼气生产的额外量的粪土。
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引用次数: 0
Research of a Dry Extruded Mixture of Protein Rich Plant Composition 一种富含蛋白质植物成分的干挤压混合物的研究
Q4 Social Sciences Pub Date : 2022-08-01 DOI: 10.2478/plua-2022-0003
V. Šterna, Enno Ence, E. Štrausa
Abstract Since plant protein is generally accepted as sustainable and environmentally friendly, production of new plant protein-based products is expanding and a raw material is diversified to ensure a high-value and complete meal. The objective of the study was to compare chemical composition and amino acid provision of pea-buckwheat, pea-barley and pea-oat extruded mixtures with the aim to look for new products to diversify diet using the best plant protein resources. Within the research, amino acid scores of hull-less barley, buckwheat, hull-less oat and grey pea as an indicator of raw material protein quality were evaluated. The highest scores of essential amino acids were found in oat and pea protein. Amino acid scoring patterns of raw material – oat ‘Stendes Emilija’ and grey pea ‘Bruno’ fully meet the requirements for essential amino acid supply. The protein of buckwheat is the richest in lysine (4.3 g 100g−1 protein) among studied cereals, although the difference in the essential amino acids was not significant. The results of the research showed that pea-barley and pea-buckwheat composite mixtures meet human needs better if pea protein is used in the proportion 65:35 with barley or buckwheat flour than in the proportion 35:65. The high protein content – 43.33% and 43.96%, the sum and composition of essential amino acids, and high level of total dietary fibre – 11.9% and 13.7% are the main reasons to consider the mixtures (including as a base for smoothies) as a complete meal.
由于植物蛋白被普遍认为是可持续的和环保的,新的植物蛋白产品的生产正在扩大,原料的多样化,以确保高价值和完整的膳食。本研究的目的是比较豌豆-荞麦、豌豆-大麦和豌豆-燕麦挤压混合物的化学成分和氨基酸供应,旨在寻找利用最佳植物蛋白资源实现日粮多样化的新产品。本研究以无壳大麦、荞麦、无壳燕麦和灰豌豆的氨基酸得分作为原料蛋白质品质的指标进行评价。燕麦和豌豆蛋白质中必需氨基酸的含量最高。原料燕麦“Stendes Emilija”和灰豌豆“Bruno”的氨基酸评分模式完全满足必需氨基酸供应要求。在所研究的谷物中,荞麦蛋白质中赖氨酸含量最高(4.3 g 100g - 1),但必需氨基酸含量差异不显著。研究结果表明,豌豆蛋白与大麦或荞麦粉以65:35的比例使用比以35:65的比例使用更能满足人类的需求。高蛋白含量(43.33%和43.96%),必需氨基酸的总和和组成,以及高水平的总膳食纤维(11.9%和13.7%)是考虑将混合物(包括作为冰沙的基础)作为完整膳食的主要原因。
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引用次数: 0
The Missing Link of UTM UTM缺失的环节
Q4 Social Sciences Pub Date : 2022-08-01 DOI: 10.2478/plua-2022-0010
R. Rumba, A. Ņikitenko
Abstract As the use cases for rural unmanned aerial vehicle (UAV) missions are evolving, the next step is to make on-site UAV missions controlled over long distances. The control is called Beyond visual line of sight (BVLOS) and there is a great need for use cases to become autonomous or self-sustaining over long distances. Designing airspace to include autonomous and BVLOS UAV involves proposals for traffic that do not exist to date, different actors, and a multitude of risks; system design must answer a wide range of questions before its release. Luckily, most of the technical questions are already solved, most of the safety regulations are there in place in general aviation, and the industry is waiting for a new type of transportation. In this paper, the authors propose action for policymakers on how to approach the challenge of developing UAV Traffic Management (UTM) systems that can be applied to any geographic location and identify missing links in decision-making to enable development.
摘要随着农村无人机任务的用例不断发展,下一步是对现场无人机任务进行远程控制。这种控制被称为超越视觉视线(BVLOS),非常需要用例在长距离内实现自主或自我维持。将空域设计为包括自主无人机和超视距无人机,涉及到迄今为止不存在的交通提案、不同的参与者和多种风险;在发布之前,系统设计必须回答一系列问题。幸运的是,大多数技术问题已经解决,通用航空的大多数安全法规已经到位,该行业正在等待一种新型的运输方式。在本文中,作者为决策者提出了行动建议,说明如何应对开发可应用于任何地理位置的无人机交通管理(UTM)系统的挑战,并确定决策中缺失的环节,以实现发展。
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引用次数: 0
Total Soluble Sugars in Root and Stem of Wichita and Western Carya Illinoinensis Varieties in Northern Mexico 墨西哥北部威奇托和西部山核桃品种根和茎中的总可溶性糖
Q4 Social Sciences Pub Date : 2022-08-01 DOI: 10.2478/plua-2022-0002
Ernesto Concilco-Alberto, L. M. Valenzuela-Núñez, Anselmo González-Torres, C. G. la Peña, Edwin Amir Briceño-Contreras, J. Rivera, J. L. Reyes-Carrillo, Mario García-Carrillo
Abstract The aim of the current study was to determine the monthly total soluble sugar concentration in the stem and root of the Wichita and Western walnut (Carya illinoinensis) varieties during a productive cycle in northern Mexico. Vegetable samples were obtained using a systematic sampling method, and processed by means of a spectrophotometric assay to determine the total soluble sugars (TSS) concentration in productive walnut trees. Data analysis was performed by means of a 2-way ANOVA. The results of the TSS concentration had differences throughout the months of an annual productive cycle, and were also different between the evaluated walnut varieties. The highest total soluble sugar concentration in stem and root were in the lowest temperature months, and it decreases in both tissues when the environmental temperature rises. The Wichita variety had a higher total soluble sugar concentration than the Western in every month during the productive cycle. Further research is required to assess the effect of other type of factors including environmental or agronomic over the dynamics of the total soluble sugars in productive walnut trees.
摘要:本研究旨在测定墨西哥北部一个生产周期内威奇托和美洲核桃(Carya illinensis)品种茎和根的月总可溶性糖浓度。采用系统取样法获得蔬菜样品,并采用分光光度法测定生产核桃树的总可溶性糖(TSS)浓度。数据分析采用双因素方差分析。TSS浓度的测定结果在一年生产周期的各个月份之间存在差异,而且在被评价的核桃品种之间也存在差异。茎和根的总可溶性糖浓度在温度最低的月份最高,随着环境温度的升高,茎和根的总可溶性糖浓度降低。威奇托品种在生产周期中每个月的总可溶性糖浓度都高于西部品种。需要进一步的研究来评估其他类型的因素,包括环境或农艺对生产核桃树总可溶性糖动态的影响。
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引用次数: 1
Impacts of COVID-19 on the Food Supply Chain for Arable Crops in Latvia 新冠肺炎对拉脱维亚农作物粮食供应链的影响
Q4 Social Sciences Pub Date : 2022-08-01 DOI: 10.2478/plua-2022-0007
I. Upīte, Dina Bite, I. Pilvere, Aleksejs Nipers
Abstract The COVID-19 pandemic has caused unprecedented stress to food supply chains, pointing to weaknesses in the labour, processing, transport and logistics spheres, as well as significant changes in demand. Food supply chains have shown considerable resilience in the face of such stress. Monitoring of the food sector during the COVID-19 pandemic indicates that the effects of COVID-19 on food supply, demand and access are interlinked processes and have a complex impact on food systems. In addition, high-value food supply chains in developed countries are even more complex, so countries need to respond quickly to restore them and also develop mechanisms that balance business and public interests and protect farmers and food business workers during future potential pandemic crises. The research aims to identify the main demand and supply side factors that affect the resilience of local and global food supply chains during the COVID-19 crisis, as well as assess the resilience of the supply chain for arable crops during the COVID-19 crisis in Latvia, identifying the main risk factors. The research found that arable crop farmers were relatively less affected by the COVID-19 crisis than other agricultural employees. The impact of the crisis was larger on the crop processing industry than on the other industries, and the main risk factors related to changes in demand and the industry’s dependence on imported raw materials and the successful operation of export markets. Based on an in-depth risk analysis, the research developed recommendations for policy makers and actors in the agri-food chain that would improve the resilience of local (domestic) participants of the arable crop food supply chain in Latvia.
2019冠状病毒病大流行给粮食供应链带来了前所未有的压力,暴露出劳动力、加工、运输和物流领域的弱点,以及需求的重大变化。面对这种压力,粮食供应链显示出相当大的弹性。2019冠状病毒病大流行期间对粮食部门的监测表明,2019冠状病毒病对粮食供应、需求和获取的影响是相互关联的过程,对粮食系统产生复杂影响。此外,发达国家的高价值食品供应链更为复杂,因此各国需要迅速作出反应,恢复供应链,并建立平衡企业和公共利益的机制,在未来潜在的大流行危机中保护农民和食品企业工人。该研究旨在确定在2019冠状病毒病危机期间影响当地和全球粮食供应链复原力的主要需求侧和供应侧因素,并评估拉脱维亚在2019冠状病毒病危机期间可耕地作物供应链的复原力,确定主要风险因素。研究发现,与其他农业从业人员相比,种植作物的农民受新冠肺炎危机的影响相对较小。危机对作物加工业的影响大于对其他行业的影响,主要风险因素与需求变化、该行业对进口原材料的依赖以及出口市场的成功运作有关。在深入风险分析的基础上,该研究为农业食品链中的政策制定者和行动者提出了建议,这些建议将提高拉脱维亚可耕地作物食品供应链的当地(国内)参与者的抵御能力。
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引用次数: 1
Sensory and Textural Properties of Frozen Muffins Prepared with Cottage Cheese and Tofu 干酪豆腐冷冻松饼的感官和质地特性
Q4 Social Sciences Pub Date : 2022-08-01 DOI: 10.2478/plua-2022-0004
D. Klava, E. Straumite, Liene Jansone, Andris Sedmalis
Abstract Muffin is a flour confectionery product with an elastic texture. Nowadays, vegetable products are replacing traditional dairy products more often in the food sector. Tofu, which is a quintessential soy protein product, is one of the alternatives for making vegan products. The aim of this research was to determine the influence of defrosting processes to changes of sensory and textural properties of a muffins with cottage cheese and its vegan substitutes. Five types of muffins were used in the study – muffin without cottage cheese (control), muffin with cottage cheese, muffin with lactose-free cottage cheese, tofu muffin and tofu chickpea muffin, which were thawed after freezing in two ways (at room temperature and in a microwave oven). For the determination of muffin quality, the Quantitative descriptive method (QDA) was used, and for overall liking, the 7-point hedonic scale and acceptance – Just-about-Right (JAR) method were used. To evaluate physical-chemical and textural analysis, standard methods were used. The method of defrosting muffins – room (ambient) temperature or microwave – has no significant effect (p>0.05) on the quality and JAR rating of the muffins with cottage cheese and tofu. The obtained results showed that the addition of plant-based protein sources significantly reduces the volume of muffins; the texture of the muffins becomes softer, but more sticky. In the process of defrosting using a microwave, the structure of the muffins is drier, softer, whitish, and less sticky, compared to a muffin thawed at 22 °C.
摘要松饼是一种具有弹性质地的面粉糖果产品。如今,在食品行业,蔬菜产品正在取代传统的乳制品。豆腐是一种典型的大豆蛋白产品,是制作纯素食产品的替代品之一。这项研究的目的是确定解冻过程对含有农家奶酪及其纯素食替代品的松饼感官和质地特性变化的影响。研究中使用了五种类型的松饼——不含农家干酪的松饼(对照)、含农家奶酪的松饼、不含乳糖的农家干酪松饼、豆腐松饼和豆腐鹰嘴豆松饼,它们在冷冻后以两种方式解冻(在室温和微波炉中)。对于松饼质量的测定,使用了定量描述法(QDA),对于整体喜好,使用了7点享乐量表和接受度-大致正确(JAR)法。为了评估物理化学和结构分析,使用了标准方法。解冻松饼的方法——室温(环境)或微波炉——对含有干酪和豆腐的松饼的质量和JAR评级没有显著影响(p>0.05)。所获得的结果表明,添加植物蛋白源显著减少了松饼的体积;松饼的质地变得更软,但更粘。在使用微波炉解冻的过程中,与在22°C下解冻的松饼相比,松饼的结构更干燥、更柔软、带白色、粘性更小。
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引用次数: 0
ATX/LPA axis regulates FAK activation, cell proliferation, apoptosis, and motility in human pancreatic cancer cells. ATX/LPA 轴调节人胰腺癌细胞的 FAK 激活、细胞增殖、凋亡和运动。
IF 2.1 Q4 Social Sciences Pub Date : 2022-04-01 Epub Date: 2022-04-14 DOI: 10.1007/s11626-022-00660-3
Yan Liao, Lei Liu, Jiayao Yang, Zhaohong Shi

Previous studies implicated ATX/LPA axis as a potential driver of tumorigenesis and progression in pancreatic cancer. This study aimed to determine the existence of the autocrine pathway of ATX/LPA action in pancreatic cancer cells, and to elucidate its influence on focal adhesion kinase (FAK) activation, cellular proliferation, apoptosis, and migration. Firstly, we identified the lysophosphatidic acid (LPA) concentrations in cultured cell supernatant by ELISA and observed the effect of the autotaxin (ATX)-specific inhibitor S32826 on LPA concentrations. We found the existence of a certain concentration of LPA in cellular supernatant, which was significantly decreased by S32826 in a dose- and time-dependent manner. A maximum response was observed at 50 μM for 72 h. Secondly, the effect of S32826 on the protein expression and intracellular sublocalization of total FAK and phosphorylated FAK (pY397 FAK) was determined by Western blot and immunofluorescence staining. It was found that the expression of total FAK and pY397 FAK and their distribution along the cell membrane where adhesion structures are located were significantly decreased by S32826. Finally, we observed the influence of S32826 on cell proliferation, apoptosis, and migration by CCK-8 assay, flow cytometric analysis, and transwell migration assay. Results showed that cell viability and migration were significantly declined, and the proportions of apoptotic cells were significantly increased by S32826. This study verified the existence of autocrine regulation of LPA secretion via producing ATX by pancreatic cancer cells in vitro and the important role of LPA/ATX axis on FAK activation, cell proliferation, apoptosis, and motility.

以前的研究表明,ATX/LPA 轴是胰腺癌肿瘤发生和发展的潜在驱动因素。本研究旨在确定 ATX/LPA 作用于胰腺癌细胞的自分泌途径,并阐明其对局灶粘附激酶(FAK)活化、细胞增殖、凋亡和迁移的影响。首先,我们用酶联免疫吸附法测定了培养细胞上清液中溶血磷脂酸(LPA)的浓度,并观察了自体交联素(ATX)特异性抑制剂S32826对LPA浓度的影响。我们发现细胞上清液中存在一定浓度的 LPA,S32826 能以剂量和时间依赖的方式显著降低 LPA 浓度。其次,通过 Western 印迹和免疫荧光染色测定了 S32826 对总 FAK 和磷酸化 FAK(pY397 FAK)蛋白表达和胞内亚定位的影响。结果发现,S32826 能显著降低总 FAK 和 pY397 FAK 的表达,并减少它们沿粘附结构所在细胞膜的分布。最后,我们通过 CCK-8 试验、流式细胞仪分析和跨孔迁移试验观察了 S32826 对细胞增殖、凋亡和迁移的影响。结果表明,S32826 会显著降低细胞活力和迁移率,并显著增加凋亡细胞的比例。本研究验证了胰腺癌细胞在体外通过产生ATX对LPA分泌进行自分泌调控,以及LPA/ATX轴对FAK活化、细胞增殖、凋亡和运动的重要作用。
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引用次数: 2
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Rural Sustainability Research
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