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Screening of Venturia inaequalis virulence and resistance of Malus genotypes to apple scab using in vitro methodology 应用离体方法筛选苹果不同基因型的不平衡Venturia毒力和对苹果赤霉病的抗性
Q4 Social Sciences Pub Date : 2022-12-01 DOI: 10.2478/plua-2022-0018
O. Sokolova, I. Moročko‐Bičevska
Abstract The apple scab induced by Venturia inaequalis is an economically significant disease of apples worldwide and is predominantly controlled by multiple fungicide applications. Therefore, resistant apple cultivation is important for long-standing disease control. The knowledge about cultivar resistance is mainly founded on their evaluation in orchard conditions and testing in a greenhouse that is laborious and requires a large space. This study evaluated apple cultivar resistance and the virulence of V. inaequalis strains by inoculating detached leaves and immature fruits. Nine V. inaequalis strains originating from different apple-growing regions and host genotypes were tested on eight apple genotypes. Microscopic and macroscopic symptom development and host tissue reactions were monitored during the experiments. The tested V. inaequalis strains and cultivars showed different levels of virulence and resistance, respectively. Cultivar ‘Lobo’ was scored as partially susceptible, with almost all strains tested. The incompatible interaction with or without host tissue reactions was observed only on the cultivar ‘Priscilla’ with all the strains and field populations tested. The results of this study using detached leaves and immature fruits were concordant with the data obtained in the cultivar evaluation trials in the orchards.
摘要苹果赤霉病(Venturia inaequalis)是世界范围内苹果的一种重要经济病害,主要采用多种杀菌剂防治。因此,抗病苹果的培育对病害的长期防治具有重要意义。对品种抗性的认识主要建立在果园条件下的评价和温室试验上,温室试验费时费力,占地面积大。本研究通过接种离体叶片和未成熟果实,对苹果品种的抗性和毒力进行了评价。对来自不同苹果产区和不同寄主基因型的9株不平等弧菌进行了8个苹果基因型的检测。在实验过程中监测微观和宏观症状发展和宿主组织反应。不同菌株和品种的毒力和抗性水平不同。品种“Lobo”被评为部分易感,几乎所有菌株都被测试过。在所有菌株和田间群体中,仅在‘普丽西拉’品种上观察到与宿主组织不相容的相互作用。用离体叶片和未成熟果实进行的研究结果与果园品种评价试验的结果一致。
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引用次数: 0
Lake Kisezers: analysis of factors influencing morphometry and hydrological regime Kisezers湖:影响地形测量和水文状况的因素分析
Q4 Social Sciences Pub Date : 2022-12-01 DOI: 10.2478/plua-2022-0017
J. Dumpis, A. Lagzdins, I. Šics
Abstract This study describes dynamics in hydrology, morphometry, and water mass movement in Lake Kisezers. The analysis of Lake Kisezers’ morphometry dynamics according to the rapidly changing environment has been carried out. The determination of dynamics in morphometry comes before understanding potential exploitation and environmental sustainability of water body. Lake Kisezers is situated in a densely populated area. Without understanding and deciphering all possible environmental factors influencing Lake Kisezers, the water body can degrade and become of poor environmental quality. Such changes can lead to irreversible changes in the lake ecosystem and degradation of the surrounding landscape. This study aims to identify and explain the elements that influence Lake Kisezers’ hydrological regime, catchment area, and bathymetry. Lake Kisezers is hardly influenced body of water with rapid hydrological and morphometric dynamics. The research is possible due to extensive data availability and the importance of the water body location. Lake Kisezers affects the city of Riga and its surrounding area with a danger of flooding and decreasing land exploration potential. This study results in better understanding in the factors influencing hydrology and morphometry in Lake Kisezers and possible environmental sustainability endangerment in the future. The main impact factor is the connectivity to the River Daugava and its unique hydrological regime. For Lake Kisezers, environmental balance is necessary to continue research and develop a hydrological change model. This study was conducted with fieldwork data and open-source cartographic, geospatial, water level, and mass movement data. The research seeks to understand what is necessary to maintain environmental sustainability in Lake Kisezers.
摘要本研究描述了Kisezers湖水文、形态计量和水团运动的动力学。根据快速变化的环境,对Kisezers湖的形态计量动力学进行了分析。在了解水体的潜在开发和环境可持续性之前,必须确定形态计量学中的动力学。Kisezers湖位于人口稠密的地区。如果不了解和解读影响Kisezers湖的所有可能的环境因素,水体可能会退化并变得环境质量差。这种变化可能导致湖泊生态系统发生不可逆转的变化,并导致周围景观退化。本研究旨在确定和解释影响Kisezers湖水文状况、集水区和水深测量的因素。Kisezers湖几乎不受水体的影响,具有快速的水文和形态动力学。由于广泛的数据可用性和水体位置的重要性,这项研究是可能的。Kisezers湖影响里加市及其周边地区,有洪水泛滥的危险,土地勘探潜力下降。这项研究有助于更好地了解影响Kisezers湖水文和地貌测量的因素,以及未来可能的环境可持续性危害。主要影响因素是与道加瓦河的连通性及其独特的水文状况。对于Kisezers湖来说,环境平衡对于继续研究和开发水文变化模型是必要的。这项研究是利用实地调查数据和开源地图、地理空间、水位和群众运动数据进行的。这项研究旨在了解维持基泽泽斯湖环境可持续性的必要条件。
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引用次数: 0
Multifunctionality of urban agriculture and its characteristics in Latvia 拉脱维亚都市农业的多功能性及其特征
Q4 Social Sciences Pub Date : 2022-12-01 DOI: 10.2478/plua-2022-0016
Madara Dobele, Aina Dobele, A. Zvirbule
Abstract In the 21st century, urban agriculture renews and diversifies approaches to the practice within the context of urban regeneration, climate change risks, sustainable development and resource consumption balance problems. Influenced by the long historical evolution and intensification of various risks, food production in urban areas involves the widest multifunctionality to date. Therefore, the aim of this research is to identify the main functions of urban agriculture and to evaluate their significance in Latvia. The following tasks were set to achieve the aim: 1) to identify and classify functions of urban agriculture, 2) to analyse the performance and significance of functions in Latvia. Several methods were employed to perform tasks and achieve the aim: the monographic and descriptive methods for making a theoretical discussion; the analysis, synthesis and deduction methods for obtaining information, systematisation and classification of functions; a structured expert interview for rating and ranking functions and identifying interactions between them. The research identified 14 functions of urban agriculture, which were classified into 5 groups: political, economic, social, environmental and technological. The most significant functions of urban agriculture in Latvia are social (promotion of social cohesion and public health, education and maintaining traditions and values) and technological innovations. There are interactions between all functions, and support for urban sustainability, education, provision of ecosystem services and technological innovations have the strongest impact on other functions. Interactions of functions proved the multifunctionality of urban agriculture, which could be an important support tool to contributing to sustainable urban development.
摘要在21世纪,城市农业在城市更新、气候变化风险、可持续发展和资源消耗平衡问题的背景下,更新并多样化了实践方法。受长期历史演变和各种风险加剧的影响,城市地区的粮食生产具有迄今为止最广泛的多功能性。因此,本研究的目的是确定城市农业的主要功能,并评估其在拉脱维亚的意义。为实现这一目标,制定了以下任务:1)确定和分类城市农业的功能,2)分析拉脱维亚功能的表现和意义。采用了几种方法来执行任务并实现目标:进行理论讨论的专题和描述性方法;获取信息的分析、综合和推导方法,功能的系统化和分类;一种结构化的专家访谈,用于对功能进行评级和排名,并确定它们之间的互动。研究确定了城市农业的14项功能,将其分为5组:政治、经济、社会、环境和技术。拉脱维亚城市农业最重要的功能是社会(促进社会凝聚力和公共卫生、教育以及维护传统和价值观)和技术创新。所有职能之间都有相互作用,支持城市可持续性、教育、提供生态系统服务和技术创新对其他职能的影响最大。功能的相互作用证明了城市农业的多功能性,它可以成为促进城市可持续发展的重要支持工具。
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引用次数: 1
Retrospective Analysis of 101 Canine Lymphoma Cases Diagnosed in Surgical biopsies in Latvia (2011–2020) 拉脱维亚2011-2020年101例犬淋巴瘤手术活检回顾性分析
Q4 Social Sciences Pub Date : 2022-12-01 DOI: 10.2478/plua-2022-0011
I. M. Houtana, Lilija Geine-Romanova
Abstract Lymphoma is a malignant tumor commonly diagnosed in dogs representing 7–24% of all canine tumors. There has been no previous studies focused on characterization of canine lymphoma cases in Latvia. The goals of this retrospective study were to determine prevalence and characteristics of lymphoma cases among biopsy submissions to a private veterinary pathology service that receives approximately 80% of all biopsy submission in Latvia and to compare this data with published canine lymphoma reviews. Data were retrieved to select records from Latvian dogs diagnosed with lymphoma between 2011 and 2020, determining characteristics of dogs and types of lymphoma based on anatomic distribution and cellular morphology. In a ten-year study period, diagnosis of lymphoma constituted 1–4% of surgical biopsy submissions each year without upwards trend. Affected dogs were middle age (median 8 years; range 2–13), with a slight male predominance (58%). The majority of dogs (19%) were mixed breed. Top 3 affected dog breeds were Rottweiler, American Staffordshire terrier and French bulldog with 6–7 cases in each breed. Multicentric form of lymphoma predominated (55%) followed by alimentary and mucocutaneous lymphoma (21% and 14%, respectively). Within multicentric form of lymphoma two thirds were intermediate to large cell lymphomas. Included in this group would be diffuse large B cell lymphomas, the most common subtype of canine lymphomas; however, lack of immunohistochemical testing precluded complete lymphoma classification according to WHO guidelines. Results of this study correlate well with the previously published results and provide important information to Latvian small animal veterinarians and pathologists.
摘要淋巴瘤是一种常见于狗的恶性肿瘤,占所有狗肿瘤的7-24%。在拉脱维亚,以前没有专门研究犬淋巴瘤病例的特征。这项回顾性研究的目的是确定向私人兽医病理服务机构提交的活检中淋巴瘤病例的患病率和特征,该机构接受了拉脱维亚约80%的活检,并将该数据与已发表的犬淋巴瘤综述进行比较。检索数据以选择2011年至2020年间被诊断为淋巴瘤的拉脱维亚犬的记录,根据解剖分布和细胞形态确定犬的特征和淋巴瘤类型。在一项为期十年的研究中,淋巴瘤的诊断占每年手术活检提交的1-4%,没有上升趋势。受影响的狗年龄为中年(中位数为8岁;范围为2-13岁),雄性占微弱优势(58%)。大多数狗(19%)是混血狗。受影响最严重的三个犬种是罗威纳犬、美国斯塔福德郡梗和法国斗牛犬,每个犬种有6-7例。多中心型淋巴瘤占主导地位(55%),其次是消化道和粘膜皮肤淋巴瘤(分别为21%和14%)。在多中心形式的淋巴瘤中,三分之二是中至大细胞淋巴瘤。这一组包括弥漫性大B细胞淋巴瘤,这是犬淋巴瘤最常见的亚型;然而,根据世界卫生组织指南,缺乏免疫组织化学检测排除了淋巴瘤的完整分类。这项研究的结果与之前发表的结果密切相关,并为拉脱维亚小动物兽医和病理学家提供了重要信息。
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引用次数: 1
Challenges for fodder production in Ukraine during the war 战争期间乌克兰饲料生产面临的挑战
Q4 Social Sciences Pub Date : 2022-12-01 DOI: 10.2478/plua-2022-0013
I. Cherevko
Abstract This study aimed to identify the most characteristic trends in the area of fodder production and the use of fodder as factors in the development of animal husbandry in Ukraine under conditions of war as the modern challenge for these industries. Russian military aggression is a powerful challenge for the economy of Ukraine, including its fodder production, as well as other countries of the world, reflecting on the aggravation of global problems of food shortages and food security. The effect of the mentioned challenge in Ukraine is superimposed on the identified negative trends and situation, which were formed before the beginning of 2022. Calculations based on them show that the occupation of large areas of Ukraine by Russian aggressors and hostilities caused a significant decrease in the production of livestock products due to a 13–17% decrease in the number of animals. Accordingly, consumption of fodder at the expense of these regions decreased by 16%. However, the situation is very dynamic as the war continues. Given the limitations of state support for the industry, until the end of the war the main efforts to improve fodder production in Ukraine should objectively be related to the maximum optimization of the structure of fodder rations by increasing the share of less energy-intensive forages. Every challenge creates new opportunities, therefore the post-war development of fodder production and use in Ukraine will become highly innovative.
摘要本研究旨在确定饲料生产领域的最具特征的趋势,以及在战争条件下,饲料的使用是乌克兰畜牧业发展的因素,这是这些行业面临的现代挑战。俄罗斯的军事侵略对乌克兰的经济,包括其饲料生产,以及世界其他国家来说都是一个强大的挑战,反映出全球粮食短缺和粮食安全问题的加剧。上述乌克兰挑战的影响叠加在2022年初之前形成的已确定的负面趋势和局势上。基于它们的计算表明,由于动物数量减少了13-17%,俄罗斯侵略者和敌对行动对乌克兰大片地区的占领导致畜产品产量大幅下降。因此,以牺牲这些地区为代价的饲料消耗量减少了16%。然而,随着战争的继续,局势非常动态。鉴于国家对该行业支持的局限性,在战争结束之前,改善乌克兰饲料生产的主要努力客观上应与通过增加能源密集度较低的饲料的份额来最大限度地优化饲料配给结构有关。每一个挑战都会带来新的机遇,因此乌克兰战后饲料生产和使用的发展将具有高度创新性。
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引用次数: 0
The Role of Cereal and Pulse Constituents and Processing Technology in Flake Texture Development: a Review 谷物和豆类成分及其加工技术在玉米薄片织构发育中的作用综述
Q4 Social Sciences Pub Date : 2022-12-01 DOI: 10.2478/plua-2022-0014
I. Lignicka, R. Galoburda
Abstract To meet the rising consumer demand for higher nutrition foods and to increase flake nutritional value and functionality, it is possible to combine cereals with pulses. For consumers, one of the most important quality parameters along with appearance and taste, is texture. To produce the appropriate quality of flakes, the knowledge about the relationship between chemical composition, processing parameters, and texture is necessary. The aim of this study was to characterise the role of cereal and pulse constituents and processing technologies in flake texture development, which could be used in flake production for achieving the appropriate hardness and crunchiness of new products. The monographic method was used to characterise the constituent and processing technology‘s role in cereal and pulse flake texture development. Information published from 2003 till 2022, in total 77 full text research articles, books and databases were analysed and summarised. Data analysed show if pulse and cereal nutritional information, mineral and vitamin content is known, it is possible to combine different cereals and pulses to provide a specific nutrient composition in the developed products. The main change in high starch products is a specific gelatinization and retrogradation behaviour of starch. Constituents such as lipids and proteins as well as processing technology and parameters, can significantly modify the properties and functionality of starch. Proteins can encapsulate starch granules, inhibit starch digestion and gelatinization, reduce starch structural order, and inhibit starch retrogradation.
为满足日益增长的消费者对高营养食品的需求,提高片状食品的营养价值和功能性,谷物与豆类的组合是可能的。对于消费者来说,除了外观和味道之外,最重要的质量参数之一就是质地。为了生产出合适质量的薄片,了解化学成分、加工参数和质地之间的关系是必要的。本研究的目的是表征谷物和豆类成分和加工技术在片状结构发展中的作用,这可以用于片状生产,以获得合适的硬度和脆度的新产品。采用专论的方法,分析了谷物和脉冲叶片的成分和加工工艺在其结构发育中的作用。从2003年到2022年出版的信息,总共77篇全文研究文章,书籍和数据库进行了分析和总结。经分析的数据表明,如果已知豆类和谷物的营养信息、矿物质和维生素含量,就有可能将不同的谷物和豆类结合起来,在开发的产品中提供特定的营养成分。高淀粉产品的主要变化是淀粉的特定糊化和退化行为。脂质和蛋白质等成分以及加工技术和参数可以显著改变淀粉的性质和功能。蛋白质可以包封淀粉颗粒,抑制淀粉消化和糊化,降低淀粉的结构秩序,抑制淀粉的降解。
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引用次数: 0
Coxiella burnetii in dairy cattle herd: farming practices and infection dynamics 奶牛群中burnetii Coxiella的养殖实践和感染动态
Q4 Social Sciences Pub Date : 2022-12-01 DOI: 10.2478/plua-2022-0012
G. Ringa-Ošleja, Marta Ločmele, Žanete Šteingolde, Linda Valkovska, Ilga Bubula, Maija Sergejeva, Artjoms Mališevs, Guntis Boikmanis, D. Cīrule, K. Kovalenko, V. Antāne, A. Bērziņš, L. Grantina-Ievina
Abstract Q fever is a widespread disease affecting reproduction in dairy cattle. Several risk factors can increase the possibility of the herd becoming infected and the persistence of infection. The aim of this study was to characterise the dynamics of C. burnetii infection in affected herds and to evaluate reproduction problems and risk factors using a questionnaire within infected and infection-free dairy herds. In total, 25 farms that were serologically positive or shedding C. burnetii DNA in milk and 14 C. burnetii infection-free farms in Latvia answered the questionnaire. Ten positive herds were studied by testing individual blood and milk samples from up to 10 animals at two times separated by 7.5 to 13 months. The number of serologically positive and suspicious animals was higher in the second sampling even though several animals were culled. In the positive herds, the percentage of dystocia, stillborn calves and abortions during the last year was significantly (p=0.001; 0.01; 0.005, respectively) higher than in the negative herds. Several significant factors were found for the presence of Q fever infection, such as the herd size, the regional population density of ruminants, artificial ventilation systems, and frequent farm visitors. Deratisation was less practised in the negative farms and the presence of ticks was observed more often in the negative farms. Only two identified risk factors can be mitigated – the ventilation system and frequent farm visitors. The other factors cannot be changed arbitrarily but they have to be taken into account by decision makers.
摘要Q热是一种广泛存在的影响奶牛繁殖的疾病。若干风险因素可增加畜群被感染的可能性和感染的持续性。本研究的目的是在受感染的奶牛群中描述伯纳蒂杆菌感染的动态特征,并在受感染和未受感染的奶牛群中使用问卷调查来评估繁殖问题和风险因素。总共有25个血清学阳性或在牛奶中脱落伯纳蒂菌DNA的农场和拉脱维亚14个无伯纳蒂菌感染的农场回答了调查问卷。对10个阳性畜群进行了研究,分别在7.5至13个月之间两次检测了最多10只动物的血液和牛奶样本。在第二次抽样中,尽管有几只动物被扑杀,但血清学阳性和可疑动物的数量仍较高。在阳性群体中,去年难产、死胎和流产的比例显著增加(p=0.001;0.01;分别为0.005),高于阴性猪群。发现存在Q热感染的几个重要因素,如畜群规模、反刍动物的区域种群密度、人工通风系统和频繁的农场访客。在阴性农场,除虫工作较少,而在阴性农场,蜱虫的存在更为常见。只有两个确定的风险因素可以减轻-通风系统和频繁的农场访客。其他因素不能随意改变,但决策者必须考虑到这些因素。
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引用次数: 0
Bumblebee pollination activity in a commercial tomato greenhouse during the winter season 冬季商业番茄温室中大黄蜂的授粉活动
Q4 Social Sciences Pub Date : 2022-12-01 DOI: 10.2478/plua-2022-0015
N. Ozols, J. Gailis, Inta Jakobija, J. Jaško, V. Zagorska
Abstract Bumblebee activity interaction with solar irradiation, solar irradiation and HPS lighting irradiation, the HPS effect of photoperiod, day temperature and hive density were assessed with tomato fruit weights in a commercial tomato glass greenhouse in 2020–2021. Pollination activity was measured by observing ten randomly chosen cv. ‘Beorange’ tomato plants and by counting bumblebee bruised tomato flowers. MANOVA was conducted between factors and bee activity, followed by Pearson’s correlation. A Mann-Whitney U test was calculated to determine the significance between tomato flower bruising levels and fruit weights, followed by Cliff’s delta (d). Pollination activity decreased mainly in December and January when solar irradiation decreased to below 110 J cm−2 day−1. Bumblebee activity was significantly affected by solar irradiation with HPS lighting (p<0.001; p<0.01). There was a significant correlation between bumblebee activity and solar irradiation (r= 0.75; p<0.05), and solar irradiation with HPS lighting (r= 0.70; p<0.05). There was no correlation between bumblebee activity and fruit weights (r= −0.20; p<0.05). Bruised flowers had significantly greater fruit weight increases (165.7 g) compared to unbruised flowers (123.4 g) (d= 0.12; p<0.05). Bee activity rates between 60% to 80% can be concluded as an effective rate for tomato growers. Bumblebees need at least 110–154 J cm−2 day−1 of solar irradiation to achieve a high pollination activity rate in temperate climate zones during the winter season.
以番茄果重为指标,研究2020-2021年大黄蜂与太阳辐照、太阳辐照和HPS光照的交互作用,以及光周期、日温度和蜂房密度对HPS的影响。通过观察10个随机选择的cv来测定授粉活性。“Beorange”番茄植株,并通过数大黄蜂伤过的番茄花。对各因素与蜜蜂活动进行方差分析,然后进行Pearson相关分析。通过Mann-Whitney U检验确定了番茄花挫伤水平与果实质量之间的显著性关系,其次是Cliff δ (d)。授粉活动主要在12月和1月太阳辐照量低于110 J cm−2 day−1时下降。太阳辐照与HPS照明显著影响大黄蜂的活动(p<0.001;p < 0.01)。大黄蜂活动与太阳辐照有显著相关性(r= 0.75;p<0.05),太阳辐照与HPS照明(r= 0.70;p < 0.05)。大黄蜂活动与果实质量之间没有相关性(r= - 0.20;p < 0.05)。与未碰伤花(123.4 g)相比,碰伤花的果实重量增加了165.7 g (d= 0.12;p < 0.05)。蜜蜂活动率在60%到80%之间可以被认为是番茄种植者的有效率。在温带气候地区,大黄蜂在冬季至少需要110-154 jcm−2 day−1的太阳照射才能达到较高的授粉活动率。
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引用次数: 0
The Quality Function in Determining the Effectiveness of Example Bioeconomics Tasks 确定范例生物经济学任务有效性的质量函数
Q4 Social Sciences Pub Date : 2022-12-01 DOI: 10.2478/plua-2022-0019
A. Levkin, G. Abuselidze, N. Berezhna, D. Levkin, T. Volkova, Yana Kotko
Abstract The aim of the publication is to develop theoretical and methodological foundations and practical recommendations for determining the efficiency of biotechnological processes with the subsequent justification of technical parameters to ensure a high level of viability of bio-objects. In the course of the conducted scientific research, the criteria of quality function were defined and formalised, in accordance with which the conditions of biotechnological processes of laser division of embryo were formulated as a single case in the realisation of the economic mechanism of biotechnology. This makes it possible to ensure a high level of cattle (cattle) productivity, reduce the time for the reproduction of livestock and reduce the costs of movement, trade and transportation of cattle. The quality function of the biotechnological process of laser division of embryo is formulated on the basis of criterion of non-exceeding of the temperature field in cells of blastomeres and the predetermined acceptable value that allowed one to take into account the main parameters and to propose the criterion of biotechnological process optimisation. As a promising task for the development of the bioeconomy as an economic mechanism in implementing biotechnologies, the necessity of determining the quality function by formalising the integral indicator of efficiency has been established, This enables one to ensure a high level of commercialisation of biotechnologies, to increase the productivity of agricultural animals, and to suggest alternative methods for the quick renewal of livestock.
摘要该出版物的目的是为确定生物技术过程的效率以及随后对技术参数的论证提供理论和方法基础以及实用建议,以确保生物物体的高水平生存能力。在进行的科学研究过程中,质量函数的标准被定义和正式化,根据这些标准,胚胎激光分裂的生物技术过程的条件被制定为实现生物技术经济机制的单一案例。这有可能确保高水平的牛(牛)生产力,减少牲畜繁殖的时间,并降低牛的流动、贸易和运输成本。胚胎激光分裂生物技术过程的质量函数是在不超过卵裂球细胞温度场的标准和允许考虑主要参数的预定可接受值的基础上制定的,并提出了生物技术过程优化的标准。作为发展生物经济的一项有希望的任务,生物经济是实施生物技术的一种经济机制,通过正式确定效率的整体指标来确定质量函数的必要性已经确立。这使人们能够确保生物技术的高水平商业化,提高农业动物的生产力,并为牲畜的快速更新提出替代方法。
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引用次数: 2
In Vitro Testing of Lactic Acid Bacteria from Commercial Yoghurts 商品酸奶乳酸菌的体外检测
Q4 Social Sciences Pub Date : 2022-08-01 DOI: 10.2478/plua-2022-0005
Jana Lakstiņa, I. Ciproviča, K. Majore
Abstract Yoghurt has been known as an excellent source for delivering viable lactic acid bacteria (LAB) in concentrations providing benefits to the human host and microbiome. Different gastrointestinal tract (GIT) simulators have been developed and are successfully used to determine the viability of LAB from fermented dairy products in vitro studies. Commercial yoghurts were tested for digestibility using the GIT IT system Labfors 5 (INFORS HT, Switzerland). The length for both the gastric phase and the small intestinal phase was 120 min each. Yoghurt LAB colony-forming units were analysed prior to the GIT simulation test, as well as after the simulation test. The survival rate of LAB was evaluated based on the differences in the viable LAB count at the beginning and at the end of the experiment. Tested yoghurts’ LAB were tolerated in the simulated gastric and small intestinal phases. Samples collected prior to and after the intestinal phase revealed that LAB had adapted and started to grow within 120 min. Our results showed the ability of LAB to recover in the intestinal phase which has been explained by the food matrix, which protects the bacteria from the elimination effect of intestinal secretions. In addition, differences in survival rates of LAB significantly influenced the overall LAB colony-forming units in the gastrointestial tract. The chemical composition of the product has an influence on the survival rate of LAB, and it should be studied more thoroughly. In vitro studies are quite different from the assay in vivo studies; however, this information provides significant data about the viability of LAB from regularly consumed products and helps to modulate the influence of LAB on human microbiota.
摘要众所周知,酸奶是一种很好的来源,可以提供浓度为人体宿主和微生物组有益的活乳酸菌(LAB)。已经开发了不同的胃肠道(GIT)模拟器,并在体外研究中成功地用于确定发酵乳制品中LAB的活力。使用GIT IT系统Labfors 5(INFORS HT,瑞士)测试商业酸奶的消化率。胃期和小肠期的长度各为120分钟。在GIT模拟测试之前以及模拟测试之后对Yoghut LAB菌落形成单元进行分析。基于实验开始和结束时存活LAB计数的差异来评估LAB的存活率。经测试的酸奶LAB在模拟胃和小肠阶段具有耐受性。在肠期前后采集的样本显示,LAB已经适应并在120分钟内开始生长。我们的结果表明,LAB在肠期恢复的能力可以用食物基质来解释,它可以保护细菌免受肠道分泌物的清除作用。此外,LAB存活率的差异显著影响胃肠道中整个LAB集落形成单位。产品的化学成分对LAB的存活率有影响,应对此进行更深入的研究。体外研究与体内试验研究有很大不同;然而,这些信息提供了关于LAB在经常食用的产品中的生存能力的重要数据,并有助于调节LAB对人类微生物群的影响。
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引用次数: 0
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Rural Sustainability Research
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